BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2149 (hsdM) DE:TYPE I RESTRICTION MODIFICATION ENZYME,
SUBUNIT M.
(623 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75221 type I restriction modification enzyme, chain m. PAB... 1247 0.0
sp|Q58617|YC20_METJA HYPOTHETICAL PROTEIN MJ1220 >gi|2129236|pir... 552 e-156
pir||C69226 type I restriction modification enzyme, subunit M - ... 480 e-134
gi|11500027 type I restriction-modification enzyme, M subunit [A... 472 e-132
sp|Q57596|Y132_METJA HYPOTHETICAL PROTEIN MJ0132 >gi|2129237|pir... 305 1e-81
gb|AAG18733.1| (AE004978) type I restriction modification enzyme... 211 2e-53
gi|10956197 type IC modification subunit [Streptococcus thermoph... 207 3e-52
gi|10954529 type I restriction enyzme ECOR124/3 I M protein [Met... 203 4e-51
pir||T09460 type I site-specific deoxyribonuclease (EC 3.1.21.3)... 203 5e-51
gb|AAF69138.1|AF228680_2 (AF228680) HsdM [Lactococcus lactis] >g... 203 5e-51
pir||C64695 type I restriction enzyme M protein - Helicobacter p... 197 3e-49
pir||T30323 type I site-specific deoxyribonuclease (EC 3.1.21.3)... 197 4e-49
pir||C71810 type I restriction enzyme (modification chain) - Hel... 192 7e-48
pir||S49395 HsdM1 protein - Mycoplasma pulmonis >gi|496158|gb|AA... 192 7e-48
gb|AAC25972.1| (AF003541) N6 adenine methylation (M) subunit hom... 187 4e-46
pir||E71886 type I restriction enzyme (modification chain) - Hel... 182 8e-45
pir||B64626 type I restriction enzyme M protein - Helicobacter p... 182 1e-44
pir||S73820 type I restriction enzyme hsdM - Mycoplasma pneumoni... 181 2e-44
pir||B81250 probable site-specific DNA-methyltransferase (adeni... 179 7e-44
gb|AAC38346.1| (AF013165) HsdM [Lactococcus lactis] 177 3e-43
pir||D81952 type I site-specific deoxyribonuclease (EC 3.1.21.3)... 176 6e-43
gb|AAC44666.1| (U46781) alxA [Pasteurella haemolytica] 175 1e-42
pir||F81152 type I restriction enzyme EcoR124II M protein NMB082... 173 7e-42
pir||JC5217 site-specific DNA-methyltransferase (adenine-specifi... 172 9e-42
pir||I41051 hsdM protein - Escherichia coli >gi|450688|emb|CAA53... 166 5e-40
sp|P10484|T1M1_ECOLI TYPE I RESTRICTION ENZYME ECOR124II M PROTE... 164 3e-39
pir||D82522 type I restriction-modification system DNA methylase... 161 2e-38
pir||E82933 type I restriction enzyme M protein, truncated homol... 161 3e-38
pir||G64114 site-specific DNA-methyltransferase (adenine-specifi... 159 7e-38
pir||C70880 probable hsdM protein - Mycobacterium tuberculosis (... 152 1e-35
pir||A82523 type I restriction-modification system DNA methylase... 152 1e-35
pir||T30327 hsdM protein - Klebsiella pneumoniae >gi|2408224|gb|... 134 3e-30
emb|CAA68057.1| (X99719) Sty SBLI [Salmonella enterica] 130 5e-29
pir||A82161 probable DNA methylase HsdM VC1769 [imported] - Vibr... 128 2e-28
emb|CAA09337.1| (AJ010745) hsdM-like gene [Klebsiella pneumoniae] 116 5e-25
pir||F83304 probable restriction-modification system protein PA2... 112 1e-23
gb|AAD47620.1| (AF153413) truncated HsdM [Lactococcus lactis] 105 1e-21
gb|AAG22013.1|AF288037_2 (AF288037) putative HsdM [Streptococcus... 101 3e-20
emb|CAA10699.1| (AJ132566) HsdM protein [Escherichia coli] 99 1e-19
pir||T28670 hypothetical protein - Salmonella choleraesuis >gi|1... 98 2e-19
pir||A47200 EcoA system protein M - Escherichia coli >gi|146396|... 94 3e-18
emb|CAA71895.1| (Y11005) StySKI methylase [Salmonella enterica] 94 4e-18
sp|Q47282|T1ME_ECOLI TYPE I RESTRICTION ENZYME ECOEI M PROTEIN (... 93 8e-18
sp|P07989|T1M_SALPO TYPE I RESTRICTION ENZYME STYSPI M PROTEIN >... 84 4e-15
pir||H71935 type I restriction enzyme (modification chain) - Hel... 83 8e-15
emb|CAA64185.1| (X94423) hypothetical protein [Staphylococcus au... 81 3e-14
sp|P08957|T1MK_ECOLI TYPE I RESTRICTION ENZYME ECOKI M PROTEIN (... 79 1e-13
gb|AAA23985.1| (L02508) restriction-modification enzyme type I M... 79 2e-13
sp|P40813|T1M_SALTY TYPE I RESTRICTION ENZYME STYSJI M PROTEIN >... 78 2e-13
pir||G64577 type I restriction enzyme M protein - Helicobacter p... 73 7e-12
>pir||E75221 type I restriction modification enzyme, chain m. PAB2149 -
Pyrococcus abyssi (strain Orsay)
>gi|5457729|emb|CAB49220.1| (AJ248283) TYPE I
RESTRICTION MODIFICATION ENZYME, SUBUNIT M. [Pyrococcus
abyssi]
Length = 623
Score = 1247 bits (3191), Expect = 0.0
Identities = 623/623 (100%), Positives = 623/623 (100%)
Query: 1 MVTPMPRSKTKTKNGVKNGVDEWKILEELQPWVVKRYLLIKEAFKDKEFTQEDVEKLFEE 60
MVTPMPRSKTKTKNGVKNGVDEWKILEELQPWVVKRYLLIKEAFKDKEFTQEDVEKLFEE
Sbjct: 1 MVTPMPRSKTKTKNGVKNGVDEWKILEELQPWVVKRYLLIKEAFKDKEFTQEDVEKLFEE 60
Query: 61 KRKELMEKGIKEPEFTTKNVGEILSILRKAGLIIARKDVYDFRRTYYRLKFPTETKISRD 120
KRKELMEKGIKEPEFTTKNVGEILSILRKAGLIIARKDVYDFRRTYYRLKFPTETKISRD
Sbjct: 61 KRKELMEKGIKEPEFTTKNVGEILSILRKAGLIIARKDVYDFRRTYYRLKFPTETKISRD 120
Query: 121 RLISLLKAAADQIRGGLDYKALLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKRY 180
RLISLLKAAADQIRGGLDYKALLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKRY
Sbjct: 121 RLISLLKAAADQIRGGLDYKALLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKRY 180
Query: 181 YNLFDEDTGKLYTWHEVVKSRETIKEMANALIKISEMNEELADLKKLVEVLGLIGFIKED 240
YNLFDEDTGKLYTWHEVVKSRETIKEMANALIKISEMNEELADLKKLVEVLGLIGFIKED
Sbjct: 181 YNLFDEDTGKLYTWHEVVKSRETIKEMANALIKISEMNEELADLKKLVEVLGLIGFIKED 240
Query: 241 NLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFAPQKAKEGEVYTPREVIRLLVELL 300
NLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFAPQKAKEGEVYTPREVIRLLVELL
Sbjct: 241 NLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFAPQKAKEGEVYTPREVIRLLVELL 300
Query: 301 DIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNL 360
DIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNL
Sbjct: 301 DIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNL 360
Query: 361 ILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSDRRI 420
ILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSDRRI
Sbjct: 361 ILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSDRRI 420
Query: 421 KHIYKYGYTSKQSADWAWVQLMLYYARRKVGIVLDTGALFRGGAEKAIRQGIVEDDLIEA 480
KHIYKYGYTSKQSADWAWVQLMLYYARRKVGIVLDTGALFRGGAEKAIRQGIVEDDLIEA
Sbjct: 421 KHIYKYGYTSKQSADWAWVQLMLYYARRKVGIVLDTGALFRGGAEKAIRQGIVEDDLIEA 480
Query: 481 IILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIR 540
IILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIR
Sbjct: 481 IILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIR 540
Query: 541 KIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEK 600
KIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEK
Sbjct: 541 KIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEK 600
Query: 601 RERELVEKAKAYIEGILGAMDNE 623
RERELVEKAKAYIEGILGAMDNE
Sbjct: 601 RERELVEKAKAYIEGILGAMDNE 623
>sp|Q58617|YC20_METJA HYPOTHETICAL PROTEIN MJ1220 >gi|2129236|pir||C64452 restriction
modification enzyme subunit M1 homolog - Methanococcus
jannaschii >gi|1592326|gb|AAB99225.1| (U67563) type I
restriction-modification enzyme 1, M subunit
[Methanococcus jannaschii]
Length = 578
Score = 552 bits (1407), Expect = e-156
Identities = 301/605 (49%), Positives = 400/605 (65%), Gaps = 44/605 (7%)
Query: 26 LEELQPWVVKRYLLIKEAFKDKEFTQEDVEKLFEEKRKELMEKGIKEPEFTTKNVGEILS 85
L ++P +K Y ++ + F FT + EK+ ++ + NV E+LS
Sbjct: 3 LRNVEPRFLKAYNILMDKFGLFPFTYDMAEKVLKDNYE---------------NVNEVLS 47
Query: 86 ILRKAGLIIARKDVYDFRRTYYRLKFPTETKI---SRDRLISLLKAAADQIRGGLDYKAL 142
L AGL+ D R+ Y++K T KI S+D+LI LLK AD IR +DYK L
Sbjct: 48 KLADAGLLEKTAKKEDKRKKIYKIKPLTTEKIEKVSKDKLIGLLKQGADLIRTQVDYKVL 107
Query: 143 LVFLFYKAISDRWMKMAQDLMKEGKT--KTQAYILTNKRYYNLFDEDTGKLYTWHEVVKS 200
L+FLF+KAISD+++ ++L KE + + Y+L N+ L+D + KLY WHEV +
Sbjct: 108 LLFLFFKAISDKYLLKVEELKKEFEDLDEEDIYVLANEEILELYDVEGKKLYVWHEVANN 167
Query: 201 RETIKEMANALIKISEMNEE-LADLKKLVEVLGLIGFIKEDNLHKLEEIVKIFNRVDFAE 259
E + NAL KI EMN+E L+ L +L++ GL + +N H ++ ++ +F+R DF+E
Sbjct: 168 PE---DFINALNKIVEMNKEKLSGLDELIKRTGLPTLFENENRHIVQHLINLFSRADFSE 224
Query: 260 FDSDILGDAYEWILSYFAPQKAKEGEVYTPREVIRLLVELLDIEDGSDILDPASGSGGML 319
DILGDAYEW L+YFAP KAKEGEVYTP EV +L+ L++ +D ILDPA GSG ML
Sbjct: 225 ASYDILGDAYEWTLNYFAPTKAKEGEVYTPIEVSKLIAHLVEPKDDEVILDPACGSGSML 284
Query: 320 IEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGI--QEFKIFEGADSL 377
IE YR+ P I+L GQE N+VTA L+KLN ILHGI ++ K+F G DSL
Sbjct: 285 IEQYRFA---------GSNPNIVLVGQERNDVTAVLAKLNFILHGINLKDAKVFIG-DSL 334
Query: 378 VNPQWEE---ELKRNGIEDGKVDYVIANPPWNQDGYDETRLS-DRRIKHIYKYGYTSKQS 433
+NP++E E+K G K D V+ANPPWNQDGYDE L + + K IY YG+ +K S
Sbjct: 335 LNPKFESFIXEVKGTG----KADKVVANPPWNQDGYDENTLKVNEKYKDIYMYGFPNKNS 390
Query: 434 ADWAWVQLMLYYARRKVGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAA 493
ADWAWVQL+ YY +K GIVLD+GALFRGG EK IR+ V+DDLIEA++LLPEKLFYN
Sbjct: 391 ADWAWVQLINYYTEKKAGIVLDSGALFRGGKEKTIRKRFVDDDLIEAVVLLPEKLFYNCP 450
Query: 494 APGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIE 553
APGII++LN NKPEERKGKILFINAS E+ KHPEV+KLN+L+DE+I KI AY+EFK+++
Sbjct: 451 APGIILILNKNKPEERKGKILFINASNEYIKHPEVKKLNKLSDENIEKIAKAYKEFKDVD 510
Query: 554 GFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELVEKAKAYI 613
GF +VV +EEIRKNDYNLNVSLY+ P +E E +DL + ++E ++ E E K Y+
Sbjct: 511 GFCKVVDIEEIRKNDYNLNVSLYISPIEEDEDVDLGEVYEELNKLHNEYLEKFEVVKGYL 570
Query: 614 EGILG 618
E I G
Sbjct: 571 EEING 575
>pir||C69226 type I restriction modification enzyme, subunit M -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2622040|gb|AAB85440.1| (AE000868) type I restriction
modification enzyme, subunit M [Methanobacterium
thermoautotrophicum]
Length = 616
Score = 480 bits (1222), Expect = e-134
Identities = 273/607 (44%), Positives = 382/607 (61%), Gaps = 47/607 (7%)
Query: 28 ELQPWVVKRYLLIKEAFKDKEFTQEDVEKLFEEKRKELMEKGIKEPEFTTKNVGEILSIL 87
+L W+ ++Y L+ + F+D+EF + E +E KE K++ +S +
Sbjct: 28 KLPKWLEEKYDLLWDKFEDREF-----------RSTEALEILGKEHLIDEKSLSAAISEM 76
Query: 88 RKAGLIIARKDVYDFRRTYYRLKFPTET----------KISRDRLISLLKAAADQIRGGL 137
RK G I RKD D RR Y L+ +E K+ R + +LK AAD IR +
Sbjct: 77 RKKGWIETRKDPDDERRRIYMLRPKSEIIQDYLTLKDDKLGRADIERILKGAADLIRTRV 136
Query: 138 DYKALLVFLFYKAISDRWM----KMAQDLMKE-GKTKTQAYILTNKRYYNLFDEDTGKLY 192
DYK +LV LF K +SD+WM K +D +KE G ++ +A + Y+ D D + Y
Sbjct: 137 DYKFILVLLFMKQMSDKWMMEYQKAYEDAIKEYGLSEEEARLEARNSAYH--DLDIKEEY 194
Query: 193 TWHEVVKSRETIK-EMANALIKISEMNEELADLKKLVEVLGLIGFIK-EDNLHKLEEIVK 250
W + K E + + A AL ++E+N D+ V+ + F + ++N L ++V+
Sbjct: 195 LWDNIRKDVENLPIKFAGALKNLAELNPAFKDV---VDAFDFVEFTQSQENREILRQLVE 251
Query: 251 IFNRVDFAEFDSDILGDAYEWILSYFAPQKAKEGEVYTPREVIRLLVELLDIEDGSDILD 310
+F+ D DILGDAYEWIL YFAP KAKEGEVYTPREVIRLLVE+LD + G + D
Sbjct: 252 LFSEKKLTNVDPDILGDAYEWILRYFAPTKAKEGEVYTPREVIRLLVEILDPKPGESVYD 311
Query: 311 PASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKI 370
PAS S GMLI +++YVKE + E + LYGQE+N T AL +N+ +H I++ I
Sbjct: 312 PASASNGMLIISHKYVKET-----YGEAERLFLYGQEVNRKTMALGSMNMYIHDIKDHHI 366
Query: 371 FEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSDRRI-KHIYKYGYT 429
G D+L+ P+++E +GI + D VIANPPWNQDGY E L + ++YG+
Sbjct: 367 AHG-DTLLYPKFKES---DGIM--RFDVVIANPPWNQDGYGEDTLKKGDYWRERFRYGFV 420
Query: 430 SKQSADWAWVQLMLYYARR--KVGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEK 487
+KQSADWAW+Q M+ A+ ++G+V+D G LFRGG EK+IR ++EDDLIEA+ILLPEK
Sbjct: 421 NKQSADWAWIQHMIASAKDDGRIGVVIDNGCLFRGGREKSIRSAVLEDDLIEAVILLPEK 480
Query: 488 LFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYR 547
LFYN APG I++LN +K EER+GK+LFINA E+ KHPEVRKLN L+D +I +I++AYR
Sbjct: 481 LFYNTGAPGAIIILNKDKDEERRGKVLFINAGEEYEKHPEVRKLNILSDGNIERILEAYR 540
Query: 548 EFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELVE 607
EF+ +GFSRVV L+EIR+NDYNLNV LY FPE+E E ID+A E+ EIE+ E+ E
Sbjct: 541 EFQGDDGFSRVVDLDEIRENDYNLNVPLYAFPEEELEDIDVAGEWMRLSEIEEELAEVDE 600
Query: 608 KAKAYIE 614
+ + Y++
Sbjct: 601 RIRGYLD 607
>gi|11500027 type I restriction-modification enzyme, M subunit [Archaeoglobus
fulgidus]
Length = 508
Score = 472 bits (1202), Expect = e-132
Identities = 250/507 (49%), Positives = 342/507 (67%), Gaps = 21/507 (4%)
Query: 114 ETKISRDRLISLLKAAADQIRGGLDYKALLVFLFYKAISDRWMKMAQD----LMKEGKTK 169
E + ++D LI K AAD IR +DYK +LV LF K +SD W + ++ LM++G +
Sbjct: 8 EIETTKDELIRACKQAADLIRTRVDYKYILVLLFLKKLSDEWKREYREALKTLMEKGVDE 67
Query: 170 TQAYILTNKRYYNLFDEDTGKLYTWHEVVKS-RETIKEMANALIKISEMNEELADLKKLV 228
+A IL R ++ FD + YTW E+ K+ E ++ AL ++E N EL + +V
Sbjct: 68 EEAKILAKDRSFHKFDYP--EKYTWEELRKNVNELPVRLSEALKLLAEKNPEL---QGVV 122
Query: 229 EVLGLIGFIK-EDNLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFAPQKAKEGEVY 287
+ L + F + DN L ++ ++F+ ++ ILGDAYEW++ YFAPQKAKEGEV+
Sbjct: 123 DRLDFLEFTRARDNFDILVQLFELFSGLNLGRASDSILGDAYEWLIGYFAPQKAKEGEVF 182
Query: 288 TPREVIRLLVELLDIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQE 347
TP EV+ L+V ++D + + DPA+G MLI AY YVKEK G +E + LYGQE
Sbjct: 183 TPSEVVELIVRIVDPKPMDSVYDPAAGYARMLIRAYDYVKEKY---GEEEVRKLFLYGQE 239
Query: 348 LNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQ 407
+N T A++K+N I+HGI++ + G D+L NP+++E G K D VIANPPWNQ
Sbjct: 240 VNPTTYAIAKMNAIVHGIKDINLVVG-DTLKNPRFKE-----GETFRKFDIVIANPPWNQ 293
Query: 408 DGYDETRLSDRRI-KHIYKYGYTSKQSADWAWVQLMLYYARRKVGIVLDTGALFRGGAEK 466
DGY E L ++YG+T KQSADWAW+Q ML A++KVG+V+D G LFRGG E
Sbjct: 294 DGYGEEELKKAEFYDERFRYGFTPKQSADWAWIQHMLASAKKKVGVVIDNGCLFRGGKEG 353
Query: 467 AIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHP 526
AIR+ +VEDDLIE +ILLPEKLFYN APG I++ N KPE RKGKILFINAS E+ KHP
Sbjct: 354 AIRKAVVEDDLIECVILLPEKLFYNTGAPGAIIIFNKQKPESRKGKILFINASNEYEKHP 413
Query: 527 EVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQI 586
EVRKLN+L ++HI KIV AYREFK+ +GF RVV +EE+RKNDYNLNV+LYVFP++E E+I
Sbjct: 414 EVRKLNRLGEKHIEKIVSAYREFKDGDGFCRVVDVEEVRKNDYNLNVTLYVFPQEEVEEI 473
Query: 587 DLAKEFKEFKEIEKRERELVEKAKAYI 613
D+AKE++E + IE+ E+ + + Y+
Sbjct: 474 DVAKEWEELRGIEREIDEVERRIEEYL 500
>sp|Q57596|Y132_METJA HYPOTHETICAL PROTEIN MJ0132 >gi|2129237|pir||D64316 restriction
modification enzyme subunit M1 homolog - Methanococcus
jannaschii >gi|1592267|gb|AAB98113.1| (U67470) type I
restriction-modification enzyme 2, M subunit
[Methanococcus jannaschii]
Length = 220
Score = 305 bits (772), Expect = 1e-81
Identities = 153/217 (70%), Positives = 181/217 (82%), Gaps = 5/217 (2%)
Query: 408 DGYDETRLSD----RRIKHIY-KYGYTSKQSADWAWVQLMLYYARRKVGIVLDTGALFRG 462
DGY+E L + RRI + + + GY KQSADWAWVQLMLY+AR+KVGIVLD+GALFRG
Sbjct: 2 DGYNEDVLKENPDVRRIYNTFVRGGYPPKQSADWAWVQLMLYFARKKVGIVLDSGALFRG 61
Query: 463 GAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREF 522
G EK IR+ IVE DLIEAIILLPEKLFYN APGI+M+LN NKPEERKGKILFINAS EF
Sbjct: 62 GKEKKIRKEIVEKDLIEAIILLPEKLFYNVTAPGIVMILNKNKPEERKGKILFINASLEF 121
Query: 523 RKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDE 582
KHPEVR+LN+L +E+I KIVD Y +++IEGFSRVV LEEIRKNDYNLNVSLYVFP +E
Sbjct: 122 EKHPEVRRLNRLGEENIDKIVDVYENWEDIEGFSRVVDLEEIRKNDYNLNVSLYVFPVEE 181
Query: 583 REQIDLAKEFKEFKEIEKRERELVEKAKAYIEGILGA 619
+E IDL KE +EFKEIEK+E+E++++ Y+EGIL A
Sbjct: 182 KEDIDLRKELEEFKEIEKKEKEVLDEVIGYVEGILRA 218
>gb|AAG18733.1| (AE004978) type I restriction modification enzyme, M subunit; RmeM
[Halobacterium sp. NRC-1]
Length = 499
Score = 211 bits (531), Expect = 2e-53
Identities = 159/518 (30%), Positives = 273/518 (52%), Gaps = 42/518 (8%)
Query: 117 ISRDRLISLLKAAADQIRGGLD---YKA-LLVFLFYKAISDRWMKMAQDLMKEGKTKTQA 172
++ D L S L AD IR +D YK +L +FYKAISD + + +++++E
Sbjct: 3 LTLDELDSHLFKCADIIRDAVDPTDYKEYILPLVFYKAISDEYEQEREEIVEEYGED--- 59
Query: 173 YILTNKRYYNLFDEDTGKLYTWHEVVKSRETIKE-MANALIKISEMNEELADL--KKLVE 229
N Y++ G + W+++ + E + E + NA + ++ N +L+ + +E
Sbjct: 60 -FADNANLYDVPIVPEG--HRWNDLRQESENVDEAINNAFTEFTQANPDLSGIFNANFME 116
Query: 230 VLGLIGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFAPQKAKEG-EVYT 288
GL +D L KL E + ++ +D D+LG+AY ++ +FA ++ K G + +T
Sbjct: 117 AGGLT----DDRLIKLVEHLSTYD-LDRDSVPPDMLGEAYMDLVRHFAEEEGKSGGQFFT 171
Query: 289 PREVIRLLVELLD-IEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQE 347
P +++L V L+D DG DP GSGGML EA +Y +E +G D + GQE
Sbjct: 172 PPHIVQLCVRLVDDFADGMTFHDPTVGSGGMLTEAAKYYREA---QGGDPSK-LTFTGQE 227
Query: 348 LNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQ 407
+N AA++++NL LH + E DSL +P + + G + + D V+AN P++
Sbjct: 228 INPDIAAIARMNLSLHTLNGE--IERGDSLASPGFTD-----GDDLERFDRVLANFPFSA 280
Query: 408 DGYDETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARR---------KVGIVLDTGA 458
D + D+ + + D+A++ + + K IV+ G
Sbjct: 281 DWAKDDLQDDQYGRFDWHTKLPRADRGDYAFIMHIAEQLKEPDCGDESGGKAAIVIPHGV 340
Query: 459 LFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINA 518
LFR E+ RQ ++E+DL+EAI+ LPE LF N + P I+VLN +KP +R+G++ FI+A
Sbjct: 341 LFRKH-EQRYRQPMLENDLVEAIVGLPENLFQNNSIPSAILVLNTDKPADREGEVQFIHA 399
Query: 519 SREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVF 578
+ E + E+ N+L D+ + IV+ + ++ E SR V +EEI +ND+NLN++LYV
Sbjct: 400 ADE-AFYEELSNQNELTDDGLDHIVENFDDWTTEERVSRTVGIEEIEENDFNLNIALYVD 458
Query: 579 PEDEREQIDLAKEFKEFKEIEKRERELVEKAKAYIEGI 616
+ E ID+ +E E +E++ E+ + ++E +
Sbjct: 459 TTEPEEDIDVNEELAELRELQAEREEIESRMTEHMEAL 496
>gi|10956197 type IC modification subunit [Streptococcus thermophilus]
>gi|11279435|pir||T44803 type I site-specific
deoxyribonuclease (EC 3.1.21.3) chain hsdM [imported] -
Streptococcus thermophilus plasmid pER35
>gi|6137148|gb|AAF04357.1| (AF177167) type IC
modification subunit [Streptococcus thermophilus]
Length = 531
Score = 207 bits (521), Expect = 3e-52
Identities = 157/531 (29%), Positives = 268/531 (49%), Gaps = 60/531 (11%)
Query: 126 LKAAADQIRGGLD---YKA-LLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILT-NKRY 180
L A+AD +RG +D YK LL +FYK +SD+ ++ + + GKT T T +
Sbjct: 9 LWASADILRGKMDASEYKNYLLGLIFYKYLSDKQLREVYE-QENGKTDTFPERSTLYAGF 67
Query: 181 YNLFDEDTGKLYT----------------WHEVVKSRE---TIKEMANALIKISEMNEEL 221
++ED L +H +K+ + ++ ++ EE
Sbjct: 68 MEWYEEDKDDLIENIQPRQGYFIQPDRLFYHYRIKADNYEFNLTDLQAGFNELERQGEEF 127
Query: 222 ADLKKLVEVLGL-IGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFAPQK 280
+ L +++ +G + + E+++ + +D E + D++GDAYE+++ FA
Sbjct: 128 SGLFSDIDLNSTKLGSNAQQRNVTITEVLRALDEIDLFEHNGDVIGDAYEYLIGMFAAGA 187
Query: 281 AKE-GEVYTPREVIRLLVELLDIEDGS----DILDPASGSGGMLIEAYRYVKEKLKKEGF 335
K+ GE YTP+ V R++ E+ I S I DPA GSG +++ RY+ +
Sbjct: 188 GKKAGEFYTPQAVSRIMSEITSIGQESRVPFHIYDPAMGSGSLMLNIRRYLIHPNQ---- 243
Query: 336 DEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGK 395
+ +GQELN T L+++NLILHG+ + ++ ++ W E E +
Sbjct: 244 -----VHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTLDADWPSE------EPYQ 292
Query: 396 VDYVIANPPWNQDGYDETR-LSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARRK--VGI 452
D V+ NPP++ + LSD R + K SK AD+A++ Y+ + +GI
Sbjct: 293 FDSVVMNPPYSAKWSAADKFLSDPRFERFGKLAPKSK--ADFAFLLHGFYHLKESGTMGI 350
Query: 453 VLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGK 512
VL G LFRGGAE IRQ ++E I+A+I LP +F+ + P +++L N+
Sbjct: 351 VLPHGVLFRGGAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPTTVIILKKNRSRR---D 407
Query: 513 ILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLN 572
+LFI+AS++F K + N L DEHI KIV Y++ ++IE ++ V + +EI++ND+NLN
Sbjct: 408 VLFIDASQDFEKQ---KNQNVLLDEHIDKIVSTYKKREDIERYAHVASFDEIQENDFNLN 464
Query: 573 VSLYVFPEDEREQIDLAKEFKEFKEIEKRERELVEKAKAYIEGILGAMDNE 623
+ YV +E E +DL + +I + ELV++ + + I ++E
Sbjct: 465 IPRYVDTFEEEEPVDLVEVNTNLLKINE---ELVQQEQTLLSLINDFSESE 512
>gi|10954529 type I restriction enyzme ECOR124/3 I M protein [Methanococcus
jannaschii] >gi|2496240|sp|Q60297|YZ42_METJA
HYPOTHETICAL PROTEIN MJECL42 >gi|2129333|pir||A64515
type I restriction enyzme ECOR124/3 I M homolog -
Methanococcus jannaschii plasmid pURB800
>gi|1522675|gb|AAC37111.1| (L77118) type I restriction
enyzme ECOR124/3 I M protein [Methanococcus jannaschii]
Length = 558
Score = 203 bits (512), Expect = 4e-51
Identities = 162/524 (30%), Positives = 268/524 (50%), Gaps = 56/524 (10%)
Query: 130 ADQIRGGLD---YK-ALLVFLFYKAISDRWMKMAQDLMKE-GKTKTQAYILTNK-RYYNL 183
AD++R ++ YK +L +F +A++ R+ + +++ +E ++ Y + R L
Sbjct: 56 ADKLRKKMEVHQYKYVVLGLIFLRALTCRFYERRKEIEEELSNPNSELYTEDPELRKMIL 115
Query: 184 FDED----TGKLYT-----WHEVVKS--RETIKEMANALIKISEMNEELADLKKLVEVLG 232
DED G LY W V++ I E+ + I+I E E+ D +L +V+
Sbjct: 116 EDEDFYLSEGVLYLPKETRWDYFVENVMSPNIGEIIDTAIEILE--EKYPD--RLKDVIP 171
Query: 233 LIGFIKEDNLHKLEEIVKIFNRVDFAEFD--SDILGDAYEWILSYFAPQKAK-EGEVYTP 289
I + H ++ F+ + F + D+ G YE+ L F + K G+ YTP
Sbjct: 172 KIYAQSPLDNHDYSYLINKFSEISFGKEHRVKDVFGRIYEYFLGKFTEVEGKLGGKFYTP 231
Query: 290 REVIRLLVELLDIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELN 349
R + +L+V++LD++ GS I DPA GSGG + A EKL++EG D + +YGQ+ +
Sbjct: 232 RSLTKLIVDVLDVKGGS-IFDPACGSGGFFVSAL----EKLEREGIDINE-LSIYGQDSD 285
Query: 350 EVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDG 409
+ L+K+NLI+ G + D ++ + ++ D DYV+ANPP+N
Sbjct: 286 PMAYRLTKMNLIIRGAE-------GDIRIDDSYHDDK----FMDMTFDYVVANPPFNDSE 334
Query: 410 YDETRLS--DRRIK-HIYKYGYTSKQSADWAWVQLMLYYA--RRKVGIVLDTGALFRGGA 464
+D R+ D R++ K +A++ W+ +Y+ K G V+ GAL G
Sbjct: 335 WDANRIKPDDPRLRIGNKKVPVPPNGNANYMWILHFIYHTAPNGKAGFVMANGALSAGNV 394
Query: 465 EKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRK 524
E IR+ I+E+DL+ I+ P KLFYN + P + + KP+ KGK+LFINA +++
Sbjct: 395 EGEIRKAIIENDLVYGIVACPPKLFYNVSLPVSLWFIRKEKPDYMKGKVLFINAKNLYKQ 454
Query: 525 HPEVRKLNQLADEHIRKIVDAYREFKEIE--------GFSRVVTLEEIRKNDYNLNVSLY 576
R+ N L +EHI+KIVD +R F+ E GF++V T++EI KN Y L Y
Sbjct: 455 IS--RRQNILTEEHIKKIVDKFRMFESGEDEDKINELGFAKVATIDEIAKNGYVLTPGRY 512
Query: 577 VFPEDEREQIDLAKEFKEFKEIEKRERELVEKAKAYIEGILGAM 620
V + E + I + KE+ E K+ + EK + ++ IL A+
Sbjct: 513 VGVKIEDDGIPFEVKMKEYSEELKKLLDEEEKLRNKVKEILDAL 556
>pir||T09460 type I site-specific deoxyribonuclease (EC 3.1.21.3) LldI chain
hsdM - Lactococcus lactis subsp. lactis plasmid pND861
>gi|2689699|gb|AAB91416.1| (AF034786) modification
subunit [Lactococcus lactis bv. diacetylactis]
Length = 531
Score = 203 bits (511), Expect = 5e-51
Identities = 150/496 (30%), Positives = 251/496 (50%), Gaps = 57/496 (11%)
Query: 126 LKAAADQIRGGLD---YKA-LLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKR-Y 180
L A+AD +RG +D YK LL +FYK +SD ++ + + GKT T T +
Sbjct: 9 LWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLREVYE-QENGKTDTFPERSTQYAGF 67
Query: 181 YNLFDEDTGKLYT----------------WHEVVKSRE---TIKEMANALIKISEMNEEL 221
++ED L +H +K+ + ++ ++ EE
Sbjct: 68 MEWYEEDKDDLIENIQPKQGYFIQPDQLFYHYRIKADNYEFNLTDLQAGFNELERQGEEF 127
Query: 222 ADLKKLVEVLGL-IGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFAPQK 280
+ L +++ +G + + E+++ + +D E + D++GDAYE+++ FA
Sbjct: 128 SGLFADIDLNSTKLGSNAQQRNVTITEVLRALDEIDLFEHNGDVIGDAYEYLIGMFAEGA 187
Query: 281 AKE-GEVYTPREVIRLLVELLDIEDGS----DILDPASGSGGMLIEAYRYVKEKLKKEGF 335
K+ GE YTP+ V R++ E+ I S I DPA GSG +++ RY+ +
Sbjct: 188 GKKAGEFYTPQAVSRIMSEITSIGQESRAPFHIYDPAMGSGSLMLNIRRYLLNPNQ---- 243
Query: 336 DEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGK 395
+ +GQELN T L+++NLILHG+ + ++ ++ W E E +
Sbjct: 244 -----VHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTLDADWPSE------EPYQ 292
Query: 396 VDYVIANPPWNQDGYDETR-LSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARRK--VGI 452
D V+ NPP++ + LSD R + K SK AD+A++ Y+ + +GI
Sbjct: 293 FDSVVMNPPYSAKWSAADKFLSDPRFERFGKLAPKSK--ADFAFLLHGFYHLKESGTMGI 350
Query: 453 VLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGK 512
VL G LFRG AE IRQ ++E I+A+I LP +F+ + P +++L N+
Sbjct: 351 VLPHGVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPTTVIILKKNRSRR---D 407
Query: 513 ILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLN 572
+LFI+AS++F K + N L DEHI KIV Y++ ++IE ++ V + +EI++ND+NLN
Sbjct: 408 VLFIDASQDFEKQ---KNQNVLLDEHIDKIVSTYKKREDIERYAHVASFDEIQENDFNLN 464
Query: 573 VSLYVFPEDEREQIDL 588
+ YV +E E +DL
Sbjct: 465 IPRYVDTFEEEEPVDL 480
>gb|AAF69138.1|AF228680_2 (AF228680) HsdM [Lactococcus lactis]
>gi|9789463|gb|AAF98315.1|AF243383_16 (AF243383) HsdM
[Lactococcus lactis subsp. lactis]
Length = 537
Score = 203 bits (511), Expect = 5e-51
Identities = 155/526 (29%), Positives = 263/526 (49%), Gaps = 61/526 (11%)
Query: 126 LKAAADQIRGGLD---YKA-LLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKR-Y 180
L A+AD +RG +D YK LL +FYK +SD ++ + + GKT T T +
Sbjct: 15 LWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLREVYE-QENGKTDTFPERSTQYAGF 73
Query: 181 YNLFDEDTGKLYT----------------WHEVVKSRE---TIKEMANALIKISEMNEEL 221
++ED L +H +K+ + ++ ++ EE
Sbjct: 74 MEWYEEDKDDLIENIQPKQGYFIQPDHLFYHYRIKADNYEFNLTDLQAGFNELERQGEEF 133
Query: 222 ADLKKLVEVLGL-IGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFAPQK 280
+ L +++ +G + + E+++ + +D E + D++GDAYE+++ FA
Sbjct: 134 SGLFADIDLNSTKLGSNAQQRNVTITEVLRALDEIDLFEHNGDVIGDAYEYLIGMFAAGA 193
Query: 281 AKE-GEVYTPREVIRLLVELLDIEDGS----DILDPASGSGGMLIEAYRYVKEKLKKEGF 335
K+ GE YTP+ V R++ E+ I S I DPA GSG +++ RY+ +
Sbjct: 194 GKKAGEFYTPQAVSRIMSEITSIGQESRAPFHIYDPAMGSGSLMLNIRRYLINPNQ---- 249
Query: 336 DEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGK 395
+ +GQELN T L+++NLILHG+ + ++ ++ W E E +
Sbjct: 250 -----VHYHGQELNTTTFNLARMNLILHGVDKERMNLNNGDTLDADWPSE------EPYQ 298
Query: 396 VDYVIANPPWNQDGYDETR-LSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARRK--VGI 452
D V+ NPP++ + LSD R + K SK AD+A++ Y+ + +GI
Sbjct: 299 FDSVVMNPPYSAKWSAADKFLSDPRFERFGKLAPKSK--ADFAFLLHGFYHLKESGTMGI 356
Query: 453 VLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGK 512
VL G LFRG AE IRQ ++E I+A+I LP +F+ + P +++L N+
Sbjct: 357 VLPHGVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPTTVIILKKNRSRR---D 413
Query: 513 ILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLN 572
+LFI+AS++F K + N L DEHI KIV Y++ ++IE ++ V + +EI++ND+NLN
Sbjct: 414 VLFIDASQDFEKQ---KNQNVLLDEHIDKIVSTYKKREDIERYAHVASFDEIQENDFNLN 470
Query: 573 VSLYVFPEDEREQIDL----AKEFKEFKEIEKRERELVEKAKAYIE 614
+ YV +E E +DL K +E+ ++E+ L+ + E
Sbjct: 471 IPRYVDTFEEEEPVDLVAVNTNLLKTNEELVQQEQVLLSLINDFSE 516
>pir||C64695 type I restriction enzyme M protein - Helicobacter pylori (strain
26695) >gi|2314576|gb|AAD08446.1| (AE000640) type I
restriction enzyme M protein (hsdM) [Helicobacter pylori
26695]
Length = 817
Score = 197 bits (496), Expect = 3e-49
Identities = 160/508 (31%), Positives = 251/508 (48%), Gaps = 76/508 (14%)
Query: 117 ISRDRLISLLKAAADQIRGGLD---YKA-LLVFLFYKAISDRWMKMAQDLMKEGKTKTQA 172
I + L S L A AD +RGG+D YK +L LF K ISD
Sbjct: 3 IKKSELYSSLWAGADSLRGGMDASEYKNYVLNLLFLKYISD------------------- 43
Query: 173 YILTNKRYYNLFDEDTGKLYTWHEVVKSRETIKEMANALIKISEMNEELADLKKLVEVLG 232
K N F E ++E + + E KE+ + L KI ++AD +L V+
Sbjct: 44 -----KARNNNFSEIEVPQGCFYEDILALEGDKEIGDKLNKIIA---KIADQNELKGVID 95
Query: 233 LIGFIKEDNLHK-------LEEIVKIFNRVDFAE---FDSDILGDAYEWILSYFAPQKAK 282
+ F L + L +VKIF + D D+LGDAYE+++ +FA + K
Sbjct: 96 SVDFNDNTKLGEGKAMMDTLSNLVKIFADLSLGAHGALDDDLLGDAYEYLMRHFASESGK 155
Query: 283 -EGEVYTPREVIRLLVELLDIEDGS----DILDPASGSGGMLIEAYRYVKEKLKKEGFDE 337
+G+ YTP EV L LL I+ + I DPA GSG +L++A EK
Sbjct: 156 SKGQFYTPSEVSLLSSLLLGIDANTRQDKSIYDPACGSGSLLLKASSLAGEK-------- 207
Query: 338 EPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADS-LVNPQWEEELKRNGIEDGKV 396
+ +YGQE + T AL ++N+ILH I +G S L NP + E NG+
Sbjct: 208 --GLTIYGQEKDISTTALCRMNMILHNSATADIAKGGSSTLSNPLFTTE---NGMLK-TF 261
Query: 397 DYVIANPPWNQDGYDETRLSDRRIKHI-------YKYGYTSKQSADWAWVQLMLYYARR- 448
DYV+ANPP++ + + D + K + ++ G +++ D+A++ ++ +
Sbjct: 262 DYVVANPPFSLKNWTDGLSIDPKSKQVINDSFNRFEDGTPPEKNGDFAFLLHIIKSLKNT 321
Query: 449 -KVGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPE 507
K ++L G LFRG AE AIR+ ++ I+ +I L LFY + P ++VL+
Sbjct: 322 GKGAVILPHGVLFRGNAEGAIRKNLLTKGYIKGVIGLAPNLFYGTSIPACVIVLDKENAR 381
Query: 508 ERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKN 567
RKG + I+AS++F+K N+L ++ ++K++D + +KEI +S++V+LEEI N
Sbjct: 382 ARKG-VFMIDASKDFKKDGNK---NRLREQDVQKMIDTFNAYKEIPYYSKMVSLEEISAN 437
Query: 568 DYNLNVSLYVF--PEDEREQIDLAKEFK 593
DYNLN+ Y+ PE E++ L K
Sbjct: 438 DYNLNIPRYIAAKPESEKDLFALINSHK 465
>pir||T30323 type I site-specific deoxyribonuclease (EC 3.1.21.3) LldI chain M
[validated] - Lactococcus lactis plasmid pIL2614
>gi|2865243|gb|AAC15897.1| (U90222) type IC modification
subunit [Lactococcus lactis]
Length = 531
Score = 197 bits (495), Expect = 4e-49
Identities = 156/533 (29%), Positives = 265/533 (49%), Gaps = 70/533 (13%)
Query: 126 LKAAADQIRGGLD---YKA-LLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKR-Y 180
L A+AD +RG +D YK LL +FYK +SD ++ + + GKT T T +
Sbjct: 9 LWASADILRGKMDASEYKNYLLGLIFYKYLSDAQLREVYE-QENGKTDTFPERSTQYAGF 67
Query: 181 YNLFDEDTGKL------------------YTWHEVVKSRE-TIKEMANALIKISEMNEEL 221
++ED L Y++ + E + ++ ++ EE
Sbjct: 68 MEWYEEDKDDLIENIQPKQGYFIQPDQLFYSYRIKADNYEFNLTDLQAGFNELERQGEEF 127
Query: 222 ADLKKLVEV----LGLIGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFA 277
+ L +++ LG ++ + E+++ + +D E + D++GDAYE+++ FA
Sbjct: 128 SGLFADIDLNSTKLGSNALLRNVTI---TEVLRALDEIDLFEHNGDVIGDAYEYLIGEFA 184
Query: 278 PQKAKE-GEVYTPREVIRLLVELLDIEDGS----DILDPASGSGGMLIEAYRYVKEKLKK 332
K+ GE YTP+ V +++ E+ I + I DPA GSG +++ RY+
Sbjct: 185 SSAGKKAGEFYTPQAVSKIMSEITSIGQETRAPFHIYDPAMGSGSLMLNIRRYL------ 238
Query: 333 EGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIE 392
+ + +GQELN T L+++NLILHGI + ++ ++ W E E
Sbjct: 239 ---NNPDQVHYHGQELNTTTFNLARMNLILHGIDKERMNLNNGDTLDADWPSE------E 289
Query: 393 DGKVDYVIANPPWNQD-GYDETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARRK-- 449
+ D V NPP++ + LSD R + K SK AD+A++ Y+ +
Sbjct: 290 PYQFDSVCMNPPYSAKWSAADQFLSDPRFERFGKLAPKSK--ADFAFLLHGFYHLKESGT 347
Query: 450 VGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEER 509
+GIVL G LFRG AE IRQ ++E I+A+I LP +F+ + P +++L N+
Sbjct: 348 MGIVLPHGVLFRGAAEGTIRQALLEMGAIDAVIGLPANIFFGTSIPTTVIILKRNRSRR- 406
Query: 510 KGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDY 569
+LFI+AS++F K + N L DEHI KIV +++ ++IE ++ V + +EI++ND+
Sbjct: 407 --DVLFIDASQDFEKR---KNQNVLLDEHIDKIVSIHKKREDIERYAHVASFDEIQENDF 461
Query: 570 NLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELVEKAKAYIEGILGAMDN 622
NLN+ YV +E E +DL +I + ELV++ + +L +DN
Sbjct: 462 NLNIPRYVDTFEEEEPVDLVAVNTNLLKINE---ELVQQEQV----LLSMIDN 507
>pir||C71810 type I restriction enzyme (modification chain) - Helicobacter
pylori (strain J99) >gi|4156043|gb|AAD07001.1|
(AE001564) TYPE I RESTRICTION ENZYME (MODIFICATION
SUBUNIT) [Helicobacter pylori J99]
Length = 815
Score = 192 bits (484), Expect = 7e-48
Identities = 154/495 (31%), Positives = 245/495 (49%), Gaps = 68/495 (13%)
Query: 117 ISRDRLISLLKAAADQIRGGLD---YKA-LLVFLFYKAISDRWMKMAQDLMKEGKTKTQA 172
I + L S L A AD +RGG+D YK +L LF K ISD
Sbjct: 3 IKKSELYSSLWAGADSLRGGMDASEYKNYVLNLLFLKYISD------------------- 43
Query: 173 YILTNKRYYNLFDEDTGKLYTWHEVVKSRETIKEMANALIKISEMNEELADLKKLVEVLG 232
K N+ E ++E + + E KE+ + L KI E DLK +++ +
Sbjct: 44 -----KAKNNMDSEIEVPQGCFYEDILALEGDKEIGDKLNKIIAKIAERNDLKGVIDSVD 98
Query: 233 LIGFIK----EDNLHKLEEIVKIFNRVDFAE---FDSDILGDAYEWILSYFAPQKAK-EG 284
K + + L +VKIF + D D+LGDAYE+++ +FA + K +G
Sbjct: 99 FNDNTKLGEGKAMIDTLSNLVKIFADLSLGAHGALDDDLLGDAYEYLMRHFASESGKSKG 158
Query: 285 EVYTPREVIRLLVELLDIEDGS----DILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPA 340
+ YTP EV LL LL I++ + I DP GSG +L++A +K
Sbjct: 159 QFYTPSEVSLLLSLLLGIDENTRQDKSIYDPTCGSGSLLLKASSLAGKK----------G 208
Query: 341 IMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADS-LVNPQWEEELKRNGIEDGKVDYV 399
+ +YGQE + T AL K+N+ILH I +G S L NP + E NG+ DYV
Sbjct: 209 LTIYGQEKDISTTALCKMNMILHNSATADIAKGGSSTLSNPLFTTE---NGMLK-TFDYV 264
Query: 400 IANPPWNQDGYDETRLSDRRIKHI-------YKYGYTSKQSADWAWVQLMLYYARR--KV 450
+ANPP++ + + D + K + ++ G +++ D+A++ ++ K
Sbjct: 265 VANPPFSLKNWTDGLSIDPKSKQVINDSFNRFEDGTPPEKNGDFAFLLHIIKSLNPTGKG 324
Query: 451 GIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERK 510
++L G LFRG AE IR+ ++ I+ +I L LFY + P ++VL+ RK
Sbjct: 325 AVILPHGVLFRGNAEAQIRKNLLMKGYIKGVIGLAPNLFYGTSIPACVIVLDKENAHARK 384
Query: 511 GKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYN 570
G + I+AS++F+K N+L ++ ++K++D + KEI +S++V+LEEI NDYN
Sbjct: 385 G-VFVIDASKDFKKDGNK---NRLREQDVQKMIDTFNALKEIPYYSKMVSLEEISLNDYN 440
Query: 571 LNVSLYVFPEDEREQ 585
LN+ Y+ + E E+
Sbjct: 441 LNIPRYIAAKQELEK 455
>pir||S49395 HsdM1 protein - Mycoplasma pulmonis >gi|496158|gb|AAA65633.1|
(L25415) restriction-modification enzyme subunit M1
[Mycoplasma pulmonis]
Length = 520
Score = 192 bits (484), Expect = 7e-48
Identities = 158/521 (30%), Positives = 258/521 (49%), Gaps = 59/521 (11%)
Query: 122 LISLLKAAADQIRGGLDYKA----LLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTN 177
LI+++K DQ+R ++ ++ FLF+K +S++ K ++ + + N
Sbjct: 7 LIAVVKKICDQLRSKMEVTEYRDYIMGFLFFKYLSEQSEKNFEEFKERVDYIKYSEFDEN 66
Query: 178 KRYYNLF-------DED--TGKLYTWHEVV----KSRETIKEMANALIKISEMNEELADL 224
+ D+D Y++ VV + + I + + KI +N EL D
Sbjct: 67 HEQFKKIKEIIIQNDDDFFLAYKYSFQNVVDMMNQGKNVIPTIEESFNKIESINSELNDE 126
Query: 225 KK-----LVEVLGL----IGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSY 275
KK L + +G I E+ ++ I+K N ++ + + D G+ YE++LS
Sbjct: 127 KKEFFKDLFTNIDFSNKNLGNIDEEKEKTIQLIIKEINTLNLSMDEVDHFGNTYEYLLSE 186
Query: 276 FAPQKAKE-GEVYTPREVIRLLVELLDIEDG--SDILDPASGSGGMLIEAYRYVKEKLKK 332
FA K+ GE YTP +V LLV+++ + DPA GSG +LI+ V + K
Sbjct: 187 FASDTGKKAGEFYTPSKVSELLVKIVSHGKNKINKAYDPACGSGSLLIKLANKVGKYNK- 245
Query: 333 EGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKI-FEGADSLVNPQWEEELKRNGI 391
+YGQE+ T L+++N IL G+ K+ D+L+NP EE
Sbjct: 246 ----------IYGQEVKTATYNLARMNFILRGVPFSKLDLRSGDTLINPLHIEE------ 289
Query: 392 EDGKVDYVIANPPWNQDGYDETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARRKVG 451
+G D ++ANPP++Q LS R + Y K AD+A++Q ML++ + G
Sbjct: 290 -EGSFDCIVANPPFSQKWNPTQELSKDRRYNSYP-SLAPKSYADFAFLQHMLFHVNKDNG 347
Query: 452 I---VLDTGALFRGG--AEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKP 506
I V G L R AE+ IR+ I++ + I+ II LP LFYN + I+V NKP
Sbjct: 348 IIASVFSLGILSRKSPKAEEDIRKYIIDKNYIDTIIFLPPNLFYNTSIESCIIVARKNKP 407
Query: 507 EERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRK 566
K +I INA++EF+ +K N L+DE+I +I A++E +E E FS+ ++ E+I K
Sbjct: 408 TNDK-RIFMINATKEFQN---AKKQNTLSDENINRIFSAWKEKREEENFSKYISYEDIVK 463
Query: 567 NDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELVE 607
N+Y+L++ Y + E D+ +F E EI K EL++
Sbjct: 464 NEYSLSMRFYDLDNFDEESEDIDIDFVE-SEIVKINEELLK 503
>gb|AAC25972.1| (AF003541) N6 adenine methylation (M) subunit homolog [Mycoplasma
pulmonis]
Length = 520
Score = 187 bits (469), Expect = 4e-46
Identities = 156/521 (29%), Positives = 256/521 (48%), Gaps = 59/521 (11%)
Query: 122 LISLLKAAADQIRGGLDYKA----LLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTN 177
LI+++K DQ+R ++ ++ FLF+K +S++ K ++ + + N
Sbjct: 7 LIAVVKKICDQLRSKMEVTEYRDYVMGFLFFKYLSEQSEKNFEEFKERVDYIKYSEFDEN 66
Query: 178 KRYYNLF-------DED--TGKLYTWHEVV----KSRETIKEMANALIKISEMNEELADL 224
+ D+D Y++ VV + + I + + KI +N EL D
Sbjct: 67 HEQFKKIKEIIIQNDDDFFLAYKYSFQNVVDMMNQGKNVIPTIEESFNKIESINSELNDE 126
Query: 225 KK-----LVEVLGL----IGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSY 275
KK L + +G I E+ ++ I++ N ++ + + D G+ YE++LS
Sbjct: 127 KKEFFKDLFTNIDFSNKNLGNIDEEKEKTIQLIIEEINTLNLSMDEVDHFGNTYEYLLSE 186
Query: 276 FAPQKAKE-GEVYTPREVIRLLVELLDIEDG--SDILDPASGSGGMLIEAYRYVKEKLKK 332
FA K+ GE YTP +V LLV+++ + DPA GSG +LI+ V + K
Sbjct: 187 FASDAGKKAGEFYTPSKVAELLVKIVSHGKNKINKAYDPACGSGSLLIKLANKVGKYNK- 245
Query: 333 EGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKI-FEGADSLVNPQWEEELKRNGI 391
+YGQE+ T L+++N IL G+ K+ D+L+NP EE
Sbjct: 246 ----------IYGQEVKTATYNLARMNFILRGVPFSKLDLRSGDTLINPLHIEE------ 289
Query: 392 EDGKVDYVIANPPWNQDGYDETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARRKVG 451
+ D ++ANPP++Q LS R + Y K AD+A++Q ML++ + G
Sbjct: 290 -EDSFDCIVANPPFSQKWNPTQELSKDRRYNPYP-SLAPKSYADFAFLQHMLFHVNKDNG 347
Query: 452 I---VLDTGALFRGG--AEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKP 506
I V G L R AE+ IR+ I++ + I+ II LP LFYN I+V NKP
Sbjct: 348 IIASVFSLGILSRISPKAEEDIRKYIIDKNYIDTIIFLPPNLFYNTGIESCIIVARKNKP 407
Query: 507 EERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRK 566
K +I INA++EF+ +K N L+DE+I +I A++E +E E FS+ ++ E+I K
Sbjct: 408 TNDK-RIFMINATKEFQN---AKKQNTLSDENINRIFSAWKEKREEENFSKYISYEDIVK 463
Query: 567 NDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELVE 607
N+Y+L++ Y + E D+ +F E EI K EL++
Sbjct: 464 NEYSLSMRFYDLDNFDEESEDIDIDFVE-SEIVKINEELLK 503
>pir||E71886 type I restriction enzyme (modification chain) - Helicobacter
pylori (strain J99) >gi|4155366|gb|AAD06378.1|
(AE001509) TYPE I RESTRICTION ENZYME (MODIFICATION
SUBUNIT) [Helicobacter pylori J99]
Length = 528
Score = 182 bits (458), Expect = 8e-45
Identities = 161/554 (29%), Positives = 274/554 (49%), Gaps = 88/554 (15%)
Query: 110 KFPTETKISRDRLISLLKAAADQIRG---GLDYKA-LLVFLFYKAISDRWMKMAQDLMKE 165
K ++R+ L + + A+++RG G D+K +L LFY+ IS+ M
Sbjct: 10 KLNNHASLARNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISEN--------MAN 61
Query: 166 GKTKTQAYILTNKRYYNLFDEDTGKLYTWHEVVKSRETIKEMA-----NAL----IKISE 216
+ + I N Y +L DE+ E+V+ + TI+E +AL +K +
Sbjct: 62 HHNEYERKIDPNFDYASLSDEEA-------EIVR-KSTIEEKGFFIPPSALFCNVLKNAP 113
Query: 217 MNEEL-ADLKKLVEVL--GLIGFIKEDNL-------------------HKLEEIVKIFNR 254
NE+L L+ + + +G E+N+ +++E++ KI
Sbjct: 114 NNEDLNVTLQNIFNEIEKSSLGTPSEENVKGLFADLDVNSNKLGSSHQNRVEKLTKILQA 173
Query: 255 V------DFAEFDSDILGDAYEWILSYFAPQKAKE-GEVYTPREVIRLL--VELLDIEDG 305
+ D+ + D+ GDAYE++++ +A K GE +TP+EV LL + L + E+
Sbjct: 174 IGGMQLGDYQQSGIDVFGDAYEYLMAMYASNAGKSGGEYFTPQEVSELLAKITLHNQENI 233
Query: 306 SDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGI 365
+ + DP GSG +L++ + + +K +G+ +GQE+N T L ++N+ LH I
Sbjct: 234 NKVYDPCCGSGSLLLQFSKVLGDKNVLKGY--------FGQEINLTTYNLCRINMFLHDI 285
Query: 366 --QEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSDRRIKHI 423
+F I G D+L++P+ E +D D +++NPP++ + +
Sbjct: 286 NYSKFHIALG-DTLLDPKHE--------DDEPFDAIVSNPPYSTKWIGDNNPLLMNDERF 336
Query: 424 YKYG-YTSKQSADWAWVQLMLYYARRK--VGIVLDTGALFRGGAEKAIRQGIVEDDLIEA 480
K G K +AD A+ ML Y + IV G L+R GAEK IR+ +V+++ I+
Sbjct: 337 NKAGALAPKNAADLAFTMHMLSYLSNQGAAAIVEFPGVLYRSGAEKKIREYLVKENFIDC 396
Query: 481 IILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIR 540
+I LPE LF+ I+VL NK ++ LFI+AS+EF K K N+L +
Sbjct: 397 VIALPENLFFGTNIATCILVLKKNKKDD---TTLFIDASKEFLKE---GKKNKLKAHNRE 450
Query: 541 KIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEK 600
KI+ Y E K I+ FS + +E+I++NDYNL+V+ YV ED +E ID+ E +I K
Sbjct: 451 KILQTYIERKAIKHFSALANIEKIQENDYNLSVNRYVEQEDTKEIIDIKALNGEISQIVK 510
Query: 601 RERELVEKAKAYIE 614
++ L ++ I+
Sbjct: 511 KQSVLRNSLESIIK 524
>pir||B64626 type I restriction enzyme M protein - Helicobacter pylori (strain
26695) >gi|2313984|gb|AAD07898.1| (AE000596) type I
restriction enzyme M protein (hsdM) [Helicobacter pylori
26695]
Length = 527
Score = 182 bits (456), Expect = 1e-44
Identities = 154/553 (27%), Positives = 274/553 (48%), Gaps = 80/553 (14%)
Query: 114 ETKISRDRLISLLKAAADQIRG---GLDYKA-LLVFLFYKAISDRWMKMAQDLMKEGKTK 169
+ + R+ L + + A+++RG G D+K +L LFY+ IS+ M + KE + +
Sbjct: 9 QASLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISEN---MTHYINKEERKR 65
Query: 170 TQAYILTNKRYYNLFDE--DTGKLYTWHE----VVKSRETIKEMANALIKISEMNEELAD 223
++ Y L DE + G+ + + + S + NA ++N L +
Sbjct: 66 DPSF-----DYAKLSDEKAERGRKHLIEQKGFFIPPSALFCNALKNACHN-EDLNVTLQN 119
Query: 224 LKKLVEVLGLIGFIKEDNL-------------------HKLEEIVKIFNRV------DFA 258
+ +E L G E+N+ +++E++ KI + D+
Sbjct: 120 IFNEIEKSSL-GTPSEENVKGLFADLDVNSNKLGSSHQNRVEKLTKILEAIGGMQLGDYL 178
Query: 259 EFDSDILGDAYEWILSYFAPQKAKE-GEVYTPREVIRLL--VELLDIEDGSDILDPASGS 315
+ D+ GDAYE++++ +A K GE +TP+EV LL + L E + + DP GS
Sbjct: 179 KSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHGQESVNKVYDPCCGS 238
Query: 316 GGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGI--QEFKIFEG 373
G +L++ + + +K +G+ +GQE+N T L ++N+ LH I +F I G
Sbjct: 239 GSLLLQFSKVLGDKNVSKGY--------FGQEINLTTYNLCRINMFLHDINYSKFHIAHG 290
Query: 374 ADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETR---LSDRRIKHIYKYGYTS 430
D+L++P+ E +D D +++NPP++ ++ ++D R
Sbjct: 291 -DTLLDPKHE--------DDEPFDAIVSNPPYSTKWVGDSNPILINDERFSPAGV--LAP 339
Query: 431 KQSADWAWVQLMLYYARRK--VGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKL 488
K +AD A+ ML Y IV G L+RG AE IR+ +V++++I+ +I LP+ L
Sbjct: 340 KNAADLAFTMHMLSYLSNSGTAAIVEFPGVLYRGNAEAKIREYLVKENVIDCVIALPDNL 399
Query: 489 FYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYRE 548
F+ + I+VL NK ++ LFI+AS+EF K K N+L + + KI+ Y E
Sbjct: 400 FFGTSIATCILVLKKNKQDD---TTLFIDASKEFVKE---GKKNKLKERNREKILQTYIE 453
Query: 549 FKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELVEK 608
KEI+ F + +E+I++NDYNL+V+ YV ED +E ID+ E +I +++ L +
Sbjct: 454 RKEIKHFCALANIEKIKENDYNLSVNRYVEQEDTKEAIDIKALNSEIAQIVEKQSALRNR 513
Query: 609 AKAYIEGILGAMD 621
++ I+ + G +
Sbjct: 514 LESIIKELEGGQN 526
>pir||S73820 type I restriction enzyme hsdM - Mycoplasma pneumoniae (strain
ATCC 29342) >gi|1674186|gb|AAB96142.1| (AE000048) type I
restriction enzyme HsdM [Mycoplasma pneumoniae]
Length = 543
Score = 181 bits (455), Expect = 2e-44
Identities = 156/537 (29%), Positives = 257/537 (47%), Gaps = 62/537 (11%)
Query: 130 ADQIRG---GLDYKA-LLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKRYYNLFD 185
AD++RG G D+K+ +L+ LFY+ +S+ K D + LT+ Y D
Sbjct: 20 ADKLRGTIDGWDFKSYVLIGLFYRFLSENLCKYFNDSERRNNPDFSYENLTDD--YEAID 77
Query: 186 --EDTG---------KLYTWHEVVKS------RETIKEMANALIKISEMNEELADLKKLV 228
+D + VVKS E + + E + EL D +
Sbjct: 78 ALKDAAIASKGFFIKPSQLFQNVVKSIRENKNNEDLNTTLRDIFDDIEKSTELGDGRSKE 137
Query: 229 EVLGL----------IGFIKEDNLHKLEEIVKIFNRVDFAEFDS---DILGDAYEWILSY 275
GL +G KL+E++ + ++ EF+ D GDAYE+++S
Sbjct: 138 SFKGLFKDFNVSEVKLGSTLTIRTEKLKELLTSIDTMELDEFEKNSIDAFGDAYEFLISM 197
Query: 276 FAPQKAKE-GEVYTPREVIRLLVELL--DIEDGSDILDPASGSGGMLIEAYRYVKEKLKK 332
+A K GE +TP+++ LL + + D+ D A GSG +L++ +K
Sbjct: 198 YAQNAGKSGGEFFTPQDISELLARIAIGKKDTVDDVYDMACGSGSLLLQV-------IKV 250
Query: 333 EGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQ--EFKIFEGADSLVNPQWEEELKRNG 390
G ++ + YGQE+N T L ++N+ILH I F I AD+L +WE+
Sbjct: 251 LGKEKTSLVSYYGQEINHTTYNLCRMNMILHNIDYANFNII-NADTLTTKEWEKHYVNCS 309
Query: 391 IEDGKVDYVIANPPWNQDGYDETR---LSDRRIKHIYKYGYTSKQSADWAWVQLMLYYAR 447
E+G + V++NPP++ + + +SD R K SK AD A+V LY
Sbjct: 310 NENG-FEVVVSNPPYSISWAGDKKSNLVSDVRFKDAGTLAPNSK--ADLAFVLHALYVLG 366
Query: 448 RK--VGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNK 505
++ IV G L+R G E+ IR+ +V+ + ++A+I LP LF + I+VL N+
Sbjct: 367 QEGTAAIVCFPGILYREGKEQTIRKYLVDQNFVDAVIQLPSNLFSTTSIATSILVLKKNR 426
Query: 506 PEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIR 565
++K I FI+ S EF + +K N+L+ ++I KIVD + KE F++ V ++IR
Sbjct: 427 --DKKDPIFFIDGSNEFVRE---KKNNRLSPKNIEKIVDCFNSKKEEANFAKSVERDKIR 481
Query: 566 KNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELVEKAKAYIEGILGAMDN 622
+++Y+L V YV E E+E++D+ EI ++++L K K I+ I DN
Sbjct: 482 ESNYDLTVGKYVNSEAEKEELDIKVLNHSIDEIVDKQKDLRTKIKDIIQDIKVDFDN 538
>pir||B81250 probable site-specific DNA-methyltransferase (adenine-specific)
(EC 2.1.1.72) Cj1553c [imported] - Campylobacter jejuni
(strain NCTC 11168) >gi|6968973|emb|CAB73544.1|
(AL139079) putative type I restriction enzyme M protein
[Campylobacter jejuni]
Length = 500
Score = 179 bits (450), Expect = 7e-44
Identities = 154/513 (30%), Positives = 253/513 (49%), Gaps = 56/513 (10%)
Query: 121 RLISLLKAAADQIRGGLD---YKALLV-FLFYKAISDRWMKMAQDLMKEGKTKTQAYILT 176
+L L +AD++R +D YK +++ +F + ISD +M+ ++L+KE
Sbjct: 8 KLEDALWKSADKLRKNIDAAEYKHIVLGLIFLRYISDSFMQKYEELLKEQDDGADPEDAD 67
Query: 177 NKRYYNLFDEDTGKLYTW-HEVVKSRETIKEMANALIKISEMNEELADLKKLVEVLGLIG 235
N+F Y + + K+ + K + A+ +I + N+ L + V
Sbjct: 68 EYLADNIFFVPEKSRYNYIRDNAKNPKIGKMLDEAMDEIEKHNDTLKGVLPKVYA----- 122
Query: 236 FIKEDNLHK--LEEIVKIFNRVDFAEFDS-DILGDAYEWILSYFAPQKAKEG-EVYTPRE 291
+DNL L E++ + + F S D+LG +E+ L FA + K+G + YTP+
Sbjct: 123 ---KDNLDSKCLGELIDLIGNIAFDTGKSTDVLGHVFEYFLGEFALAEGKQGGQFYTPKC 179
Query: 292 VIRLLVELLDIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEV 351
V+ LLV +L+ G + DP GSGGM +++ +VK + D+ I +YGQE N+
Sbjct: 180 VVELLVTMLEPYKGR-VFDPCCGSGGMFVQSEEFVKSHQGR--LDD---ISIYGQESNQT 233
Query: 352 TAALSKLNLILHGIQEFKIFEGADSLVNPQWEEE--LKRNGIEDGKVDYVIANPPWNQDG 409
T L+K+NL + I+ ++ W E + +D K D++IANPP+N
Sbjct: 234 TYKLAKMNLAIRKIESSQVI----------WNNEGSFLNDAHKDLKADFIIANPPFNDSD 283
Query: 410 YDETRL-SDRRIKHIYKYGYTSKQSADWAWVQLMLYYARRKVGI---VLDTGALFRGGA- 464
+ L +D R +KYG +A++AW+Q LY+ G+ VL GAL
Sbjct: 284 WSGELLENDGR----WKYGVPPASNANYAWIQHFLYHLSPNGGVAGFVLAKGALTSNTTN 339
Query: 465 EKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINA----SR 520
E AIR+ ++EDDLI+ I+ LP KLF N P + + K + K LFI+A +R
Sbjct: 340 EAAIRKALIEDDLIDCIVNLPAKLFLNTGIPASLWFIRRQKLPKTVKKTLFIDARDLGTR 399
Query: 521 EFRKHPEVRK--LNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVF 578
R++ + K +NQ+A +I K ++++I+GF + V+++EIR+ Y L YV
Sbjct: 400 INRRNKTLNKDDINQIA--NIYKAWKNGTDYEDIKGFCKSVSIDEIRELSYVLTPGRYVG 457
Query: 579 PEDEREQIDLAKEFKE----FKEIEKRERELVE 607
D ++ D F E K K E+EL E
Sbjct: 458 LADSDDEFDFDTRFNELLAKLKSQIKTEQELSE 490
>gb|AAC38346.1| (AF013165) HsdM [Lactococcus lactis]
Length = 515
Score = 177 bits (445), Expect = 3e-43
Identities = 120/369 (32%), Positives = 194/369 (52%), Gaps = 38/369 (10%)
Query: 248 IVKIFNRVDFAEFD----SDILGDAYEWILSYFAPQKAKE-GEVYTPREVIRLLVELLDI 302
++ + + EFD D + D YE++L FA A + G+ YTP+E+ ++ +L
Sbjct: 147 MISMIELLSAPEFDLSTGGDTVSDIYEYLLEKFATVLASDMGQYYTPKEISEVMARILTF 206
Query: 303 ----EDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKL 358
ED I DPA GS +LI ++K ++ AI +GQE + L+++
Sbjct: 207 GKADEDNFSIYDPAVGSASLLITTASHMKHS------NQRGAIKYFGQEKDATPYRLARM 260
Query: 359 NLILHGIQEFKI-FEGADSLVNPQWEEELKRNGIEDGK-----VDYVIANPPWNQDGYDE 412
NL++H I+ I AD+L + W + G+ +GK D V+ANPP++ ++
Sbjct: 261 NLMMHNIEYNDIQIHHADTLES-DWPD-----GVIEGKDTPRMFDAVMANPPYSAHWNNK 314
Query: 413 TRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARR--KVGIVLDTGALFRGGAEKAIRQ 470
R D R + +YG K AD++++ LY+ + +V I+L G LFRG AE IR+
Sbjct: 315 DREDDPRFR---EYGIAPKTKADYSFLLHCLYHTKESGRVAIILPHGVLFRGAAEGRIRK 371
Query: 471 GIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRK 530
+++ IEA+I P+KLF N P +++L N+ ILF++AS+ F K ++
Sbjct: 372 ALIDKHQIEAVIGFPDKLFLNTGIPVCVLILKKNR---ANSDILFVDASQGFEK---MKN 425
Query: 531 LNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAK 590
QL E I KI + K ++ +S + TLEE+ +NDYNLN+ YV +E E IDLA
Sbjct: 426 QKQLRPEDIDKITETVIHRKAVDKYSHLATLEEVIENDYNLNIPRYVDTFEEEESIDLAD 485
Query: 591 EFKEFKEIE 599
+ E++
Sbjct: 486 IQGQIDEVD 494
>pir||D81952 type I site-specific deoxyribonuclease (EC 3.1.21.3) NMA1038
[imported] - Neisseria meningitidis (group A strain
Z2491) >gi|7379740|emb|CAB84307.1| (AL162754) putative
type I restriction-modification system protein
[Neisseria meningitidis Z2491]
Length = 514
Score = 176 bits (442), Expect = 6e-43
Identities = 158/522 (30%), Positives = 246/522 (46%), Gaps = 77/522 (14%)
Query: 130 ADQIRG---GLDYKA-LLVFLFYKAISDRWMKMAQ-----------------DLMKEGKT 168
AD++RG G D+K +L LFY+ IS+ + Q +K+
Sbjct: 19 ADEVRGAVDGWDFKQYVLGTLFYRFISENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAV 78
Query: 169 KTQAY-ILTNKRYYNLFDEDTGKLYTWHEVVKSRETIKEMANALIKISEMNEELADLKKL 227
K + Y I + + N+ E H+ + +KE+ A+ + D+K L
Sbjct: 79 KVKGYFIYPGQLFCNIAAEA-------HQNEELNTKLKEIFTAIESSASGYPSEQDIKGL 131
Query: 228 VE----VLGLIGFIKEDNLHKLEEIVKIFNRVDFAEFDS---DILGDAYEWILSYFAPQK 280
+ +G D +L ++K +DF F+ D+ GDAYE+++S +A
Sbjct: 132 FDDFDTTSSRLGSTVADKNKRLAAVLKGVAELDFGSFEDHHIDLFGDAYEYLISNYAANA 191
Query: 281 AKEG-EVYTPREVIRLLVELL--DIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDE 337
K G E +TP+ V +L+ L E + I DPA GSG +L++A + E + +EGF
Sbjct: 192 GKSGGEFFTPQSVSKLIARLAVHGQEKVNKIYDPACGSGSLLLQAKKQFDEHIIEEGF-- 249
Query: 338 EPAIMLYGQELNEVTAALSKLNLILHGIQEFKI-FEGADSLVNPQWEEELKRNGIEDGK- 395
+GQE+N T L+++N+ LH + K E D+L NP+ ++D K
Sbjct: 250 ------FGQEINHTTYNLARMNMFLHNVNYNKFHIELGDTLTNPK---------LKDSKP 294
Query: 396 VDYVIANPP----WNQDGYDETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYY--ARRK 449
D V++NPP W G D T ++D R SK AD+A++ L Y R +
Sbjct: 295 FDAVVSNPPYSINWIGSG-DPTLINDDRFAPAGVLAPKSK--ADFAFILHALNYLSGRGR 351
Query: 450 VGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEER 509
IV G +RGGAE+ IRQ +VE + +E +I L LFY I+VL+ +K
Sbjct: 352 AAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFYGTGIAVNILVLSKHKDNT- 410
Query: 510 KGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDY 569
I FI+A F+K N L +EHI +IV + + ++ ++ + ++ N Y
Sbjct: 411 --DIQFIDAGGFFKKETNN---NVLTEEHIAEIVKLFADKADVPHIAQNAAQQTVKDNGY 465
Query: 570 NLNVSLYVFPEDEREQIDLAKEFKEFKE----IEKRERELVE 607
NL VS YV PED RE ID+ + E E IE+ RE+ E
Sbjct: 466 NLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDE 507
>gb|AAC44666.1| (U46781) alxA [Pasteurella haemolytica]
Length = 616
Score = 175 bits (440), Expect = 1e-42
Identities = 160/566 (28%), Positives = 272/566 (47%), Gaps = 100/566 (17%)
Query: 120 DRLISLLKAAADQIRGGLD---YKALLV-FLFYKAISDRWMKMAQDLMKEGKTKTQAYIL 175
+ L L AAD++R LD YK +++ F+F K ISD + + K KTQ
Sbjct: 82 NELDQTLWTAADKLRKNLDAANYKHIVLGFIFLKYISDSFTDF------QAKLKTQLTTP 135
Query: 176 TNKRYYN--LFDED----------------TGKLYTWHEVVKSRETIKEMANALI----- 212
++ Y + LFDE + W + IK ++ +
Sbjct: 136 ESELYLDPALFDEQEFSQILAEELEQRDYYAAENIFWVPEQARWDNIKSLSKLNLGDELP 195
Query: 213 ---KISEMNEELADLKKLVE---------VLGLIGFIKEDNLHKLEEIVKIFNRVDFAE- 259
K ++ + D + +E + + GF D + L ++ +F+R +F +
Sbjct: 196 WGDKFKGVSRLIDDAFEAIERENPKLKGVLQRIAGFGVPDEM--LTGLIDLFSRTNFTQP 253
Query: 260 ---------FDSDILGDAYEWILSYFAPQKAKEG-EVYTPREVIRLLVELLDIEDGSDIL 309
DILG YE+ L FA + K+G + +TP+ ++ L+VE+L+ G I
Sbjct: 254 MHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGGQYFTPKSIVTLIVEMLEPYSGR-IY 312
Query: 310 DPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFK 369
DPA GSGG ++A R+++ AI +YGQE N T L+ +N+ + GI
Sbjct: 313 DPAMGSGGFFVQADRFIQAHAGNRN-----AISVYGQESNSTTRKLAVMNMAIRGIPFDF 367
Query: 370 IFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQ-DGYDETRLSDRRIKHIYKYGY 428
+ D+L+NP D K+D V+ANPP+NQ + ++E+ +D R + YG
Sbjct: 368 GDKPEDTLLNPLHI---------DKKMDVVMANPPFNQKEWWNESLANDPR----WAYGT 414
Query: 429 TSKQSADWAWVQLMLYYA--RRKVGIVLDTGALF-RGGAEKAIRQGIVEDDLIEAIILLP 485
+ +A++AW+Q M+Y+ + K+ ++ G++ + E IR+ IV+ DL+EA+I LP
Sbjct: 415 PPQGNANFAWLQHMIYHLSPKGKMALLPRNGSMSSQTSGEGDIRKNIVQADLVEAMIALP 474
Query: 486 EKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDA 545
+LF N P I ++ NK + RKG++LFINA++ + + R L + I KI D
Sbjct: 475 NQLFTNTQIPACIWII--NKAKARKGEVLFINATQ--IGYLKDRVLRDFTADDIAKISDT 530
Query: 546 YREFKEIEGFSRV------VTLEEIRKNDYNLNVSLYV-FPEDEREQIDLAKEFKE---- 594
Y +++ G+ + TL+EI KND+ L YV ++E + + A++ +E
Sbjct: 531 YHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEENDGVRFAEKMQELTAL 590
Query: 595 ----FKEIEKRERELVEKAKAYIEGI 616
FK+ + E+++ E K GI
Sbjct: 591 LNEQFKQGRELEQQIAENLKGLGYGI 616
>pir||F81152 type I restriction enzyme EcoR124II M protein NMB0829 [imported] -
Neisseria meningitidis (group B strain MD58)
>gi|7226064|gb|AAF41241.1| (AE002436) type I restriction
enzyme EcoR124II M protein [Neisseria meningitidis MC58]
Length = 514
Score = 173 bits (433), Expect = 7e-42
Identities = 158/522 (30%), Positives = 251/522 (47%), Gaps = 77/522 (14%)
Query: 130 ADQIRG---GLDYKA-LLVFLFYKAISDRWMKMAQ-----------------DLMKEGKT 168
AD++RG G D+K +L LFY+ IS+ + Q +K+
Sbjct: 19 ADEVRGAVDGWDFKQYVLGTLFYRFISENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAV 78
Query: 169 KTQAY-ILTNKRYYNLFDEDTGKLYTWHEVVKSRETIKEMANALIKISEMNEELADLKKL 227
K + Y I + + N+ E H+ + +KE+ A+ + D+K L
Sbjct: 79 KVKGYFIYPGQLFCNIAAEA-------HQNEELNTKLKEIFTAIESSASGYPSEQDIKGL 131
Query: 228 VE----VLGLIGFIKEDNLHKLEEIVKIFNRVDFAEFDS---DILGDAYEWILSYFAPQK 280
+ +G D +L ++K +DF F++ D+ GDAYE+++S +A
Sbjct: 132 FDDFDTTSSRLGSTVADKNKRLAAVLKGVAELDFGNFENHHIDLFGDAYEYLISNYAANA 191
Query: 281 AKEG-EVYTPREVIRLLVELL--DIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDE 337
K G E +TP+ V +L+ L E + I DPA GSG +L++A + E + +EGF
Sbjct: 192 GKSGGEFFTPQSVSKLIARLAVHGQEKVNKIYDPACGSGSLLLQAKKQFDEHIIEEGF-- 249
Query: 338 EPAIMLYGQELNEVTAALSKLNLILHGIQ--EFKIFEGADSLVNPQWEEELKRNGIEDGK 395
+GQE+N T L+++N+ LH + +F I E D+L NP+ ++D K
Sbjct: 250 ------FGQEINHTTYNLARMNMFLHNVNYNQFHI-ELGDTLTNPK---------LKDSK 293
Query: 396 -VDYVIANPPW--NQDGYDE-TRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYY--ARRK 449
D +++NPP+ N G D+ T ++D R SK AD+A++ L Y R +
Sbjct: 294 PFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSK--ADFAFILHALNYLSGRGR 351
Query: 450 VGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEER 509
IV G +RGGAE+ IRQ +VE + +E +I L LFY I+VL+ +K
Sbjct: 352 AAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFYGTGIAVNILVLSKHKDNT- 410
Query: 510 KGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDY 569
I FI+AS F+K N L +EHI +IV + + ++ ++ + ++ N Y
Sbjct: 411 --DIQFIDASGFFKKE---TNNNVLIEEHIAEIVKLFADKADVPHIAQNAAQQTVKDNGY 465
Query: 570 NLNVSLYVFPEDEREQIDLAKEFKEFKE----IEKRERELVE 607
NL VS YV ED RE ID+ + E E IE+ RE+ E
Sbjct: 466 NLAVSSYVEAEDTREIIDIKQLNAEIGETVAKIERLRREIDE 507
>pir||JC5217 site-specific DNA-methyltransferase (adenine-specific) (EC
2.1.1.72) HsdM [validated] - Pasteurella haemolytica
Length = 380
Score = 172 bits (432), Expect = 9e-42
Identities = 126/402 (31%), Positives = 213/402 (52%), Gaps = 53/402 (13%)
Query: 245 LEEIVKIFNRVDFAE----------FDSDILGDAYEWILSYFAPQKAKEG-EVYTPREVI 293
L ++ +F+R +F + DILG YE+ L FA + K+G + +TP+ ++
Sbjct: 2 LTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGGQYFTPKSIV 61
Query: 294 RLLVELLDIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTA 353
L+VE+L+ G I DPA GSGG ++A R+++ AI +YGQE N T
Sbjct: 62 TLIVEMLEPYSGR-IYDPAMGSGGFFVQADRFIQAHAGNRN-----AISVYGQESNSTTR 115
Query: 354 ALSKLNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQ-DGYDE 412
L+ +N+ + GI + D+L+NP D K+D V+ANPP+NQ + ++E
Sbjct: 116 KLAVMNMAIRGIPFDFGDKPEDTLLNPLHI---------DKKMDVVMANPPFNQKEWWNE 166
Query: 413 TRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYA--RRKVGIVLDTGALF-RGGAEKAIR 469
+ +D R + YG + +A++AW+Q M+Y+ + K+ ++ G++ + E IR
Sbjct: 167 SLANDPR----WAYGTPPQGNANFAWLQHMIYHLSPKGKMALLPRNGSMSSQTSGEGDIR 222
Query: 470 QGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVR 529
+ IV+ DL+EA+I LP +LF N P I ++ NK + RKG++LFINA++ + + R
Sbjct: 223 KNIVQADLVEAMIALPNQLFTNTQIPACIWII--NKAKARKGEVLFINATQ--IGYLKDR 278
Query: 530 KLNQLADEHIRKIVDAYREFKEIEGFSRV------VTLEEIRKNDYNLNVSLYV-FPEDE 582
L + I KI D Y +++ G+ + TL+EI KND+ L YV ++E
Sbjct: 279 VLRDFTADDIAKISDTYHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEE 338
Query: 583 REQIDLAKEFKE--------FKEIEKRERELVEKAKAYIEGI 616
+ + A++ +E FK+ + E+++ E K GI
Sbjct: 339 NDGVRFAEKMQELTALLNEQFKQGRELEQQIAENLKGLGYGI 380
>pir||I41051 hsdM protein - Escherichia coli >gi|450688|emb|CAA53205.1| (X75452)
hsdM gene of EcoprrI [Escherichia coli]
Length = 520
Score = 166 bits (417), Expect = 5e-40
Identities = 119/372 (31%), Positives = 193/372 (50%), Gaps = 33/372 (8%)
Query: 256 DFAEFDSDILGDAYEWILSYFAPQKAKEG-EVYTPREVIRLLVELL--DIEDGSDILDPA 312
DF E D+ GDAYE+++S +A K G E +TP+ V +L+ +L + I DPA
Sbjct: 168 DFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHVNKIYDPA 227
Query: 313 SGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKI-F 371
+GSG +L++A + + + +EGF +GQE+N T L+++N+ LH I K
Sbjct: 228 AGSGSLLLQAKKQFDDHIIEEGF--------FGQEINHTTYNLARMNMFLHNINYDKFDI 279
Query: 372 EGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGY---DETRLSDRRIKHIYKYGY 428
+ ++L P + +E D +++NPP++ D T ++D R
Sbjct: 280 KLGNTLTEPHFRDEKP--------FDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAP 331
Query: 429 TSKQSADWAWVQLMLYY--ARRKVGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPE 486
SK AD+A+V L Y A+ + IV G +RGGAE+ IRQ +V+++ +E +I L
Sbjct: 332 KSK--ADFAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAP 389
Query: 487 KLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAY 546
LF+ I+VL+ +K + K+ FI+AS F+K N L D HI +I+ +
Sbjct: 390 NLFFGTTIAVNILVLSKHKTDT---KVQFIDASELFKKETNN---NILTDAHIEQIMQVF 443
Query: 547 REFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELV 606
+++ ++ V E + NDYNL+VS YV +D RE ID+A+ E K + +L
Sbjct: 444 ASKEDVAHLAKSVAFETVVANDYNLSVSSYVEAKDTREIIDIAELNAELKTTVSKIDQLR 503
Query: 607 EKAKAYIEGILG 618
+ A + I G
Sbjct: 504 KDIDAIVAEIEG 515
>sp|P10484|T1M1_ECOLI TYPE I RESTRICTION ENZYME ECOR124II M PROTEIN >gi|78917|pir||S02166
type I site-specific deoxyribonuclease (EC 3.1.21.3)
EcoR124/3 chain hsdM - Escherichia coli plasmid R124/3
>gi|4467402|emb|CAA31541.1| (X13145) hsdM protein (AA
1-520) [Escherichia coli]
Length = 520
Score = 164 bits (411), Expect = 3e-39
Identities = 118/372 (31%), Positives = 191/372 (50%), Gaps = 33/372 (8%)
Query: 256 DFAEFDSDILGDAYEWILSYFAPQKAKEG-EVYTPREVIRLLVELL--DIEDGSDILDPA 312
DF E D+ GDAYE+++S +A K G E +TP+ V +L+ +L + I DPA
Sbjct: 168 DFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHVNKIYDPA 227
Query: 313 SGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKI-F 371
+GSG +L++A + + +EGF +GQE+N T L+++N+ LH I K
Sbjct: 228 AGSGSLLLQAKKQFDNHIIEEGF--------FGQEINHTTYNLARMNMFLHNINYDKFDI 279
Query: 372 EGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGY---DETRLSDRRIKHIYKYGY 428
+ ++L P + +E D +++NPP++ D T ++D R
Sbjct: 280 KLGNTLTEPHFRDEKP--------FDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAP 331
Query: 429 TSKQSADWAWVQLMLYY--ARRKVGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPE 486
SK AD+A+V L Y A+ + IV G +RGGAE+ IRQ +V+++ +E +I L
Sbjct: 332 KSK--ADFAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAP 389
Query: 487 KLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAY 546
LF+ I+VL+ +K + + FI+AS F+K N L D HI +I+ +
Sbjct: 390 NLFFGTTIAVNILVLSKHKTDTN---VQFIDASELFKKETNN---NILTDAHIEQIMQVF 443
Query: 547 REFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELV 606
+++ ++ V E + NDYNL+VS YV +D RE ID+A+ E K + +L
Sbjct: 444 ASKEDVAHLAKSVAFETVVANDYNLSVSSYVEAKDNREIIDIAELNAELKTTVSKIDQLR 503
Query: 607 EKAKAYIEGILG 618
+ A + I G
Sbjct: 504 KDIDAIVAEIEG 515
>pir||D82522 type I restriction-modification system DNA methylase XF2742
[imported] - Xylella fastidiosa (strain 9a5c)
>gi|9107980|gb|AAF85527.1|AE004080_9 (AE004080) type I
restriction-modification system DNA methylase [Xylella
fastidiosa]
Length = 519
Score = 161 bits (404), Expect = 2e-38
Identities = 142/480 (29%), Positives = 227/480 (46%), Gaps = 64/480 (13%)
Query: 129 AADQIRGGL---DYKAL-LVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKRYYNLF 184
AAD++RG + DYK + L +F K ISD + L+ E +
Sbjct: 25 AADKLRGNMEPSDYKHVALGLIFLKYISDAFEAKHSALLAEDAQAAE------------- 71
Query: 185 DEDTGKLYTWHEVVKSRETIKEMANALIKI--SEMNEELADLKK----LVEVLGLIGFIK 238
D+D + V K ANA + ++E + D++K L VL
Sbjct: 72 DKDEYLAHNVFWVPKQARWSHLKANAKQSTIGTLIDEAMRDIEKDNPSLKHVLPKDYARP 131
Query: 239 EDNLHKLEEIVKIFNRVDFAEFDS---DILGDAYEWILSYFAPQKAKEG-EVYTPREVIR 294
N L E++ + + + E + DILG YE+ L FA + K G E YTPR V+R
Sbjct: 132 ALNKVMLGELIDLISGIALNEEGARSKDILGRVYEYFLGQFAGAEGKRGGEFYTPRSVVR 191
Query: 295 LLVELLDIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAA 354
+LV++L+ G + DP GSGGM +++ ++V E + G I +YGQE N T
Sbjct: 192 VLVQMLEPYSGR-VYDPCCGSGGMFVQSEKFVLEHGGRIG-----DIAIYGQESNYTTWR 245
Query: 355 LSKLNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETR 414
L+K+NL + GI DS + E + + D K DY++ANPP+N + R
Sbjct: 246 LAKMNLAVRGI---------DSDIRWNNEGSFHNDALRDLKADYILANPPFNISDWGGDR 296
Query: 415 LSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARRK--VGIVLDTGALFRG-GAEKAIRQG 471
L R +K+G +A++AW+Q + ++ G+VL G++ E IR
Sbjct: 297 L---REDVRWKFGVPPAGNANYAWLQHIYHHLAPNGTAGVVLANGSMSSNHSGEGEIRTH 353
Query: 472 IVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKP-----EERKGKILFINASREFRKHP 526
++E D+++ +I +P +LFY+ P + L NK +R+G++L ++A
Sbjct: 354 MIEADIVDCMIAMPGQLFYSTQIPACLWFLARNKNPGKGLRDRRGQVLLMDARALGVLVD 413
Query: 527 EVRKLNQLADEHIRKIVDAYREFK---------EIEGFSRVVTLEEIRKNDYNLNVSLYV 577
R+ +L DEHI++I D Y ++ ++ GF + TLE+IRK Y L YV
Sbjct: 414 RTRR--ELTDEHIQQIADTYHAWRGEQGAAAYADVAGFCKSATLEDIRKQGYVLTPGRYV 471
>pir||E82933 type I restriction enzyme M protein, truncated homolog UU098
[imported] - Ureaplasma urealyticum
>gi|6899053|gb|AAF30504.1|AE002110_2 (AE002110) type I
restriction enzyme M protein (fragment) [Ureaplasma
urealyticum]
Length = 348
Score = 161 bits (402), Expect = 3e-38
Identities = 123/367 (33%), Positives = 202/367 (54%), Gaps = 49/367 (13%)
Query: 264 ILGDAYEWILSYFAPQKAKEG-EVYTPREVIRLLVELLDIEDGSD----------ILDPA 312
+ GDAYE+++S +A K G E +TP+EV LLVEL I+ ++ + DP
Sbjct: 1 MFGDAYEYLMSMYAANAGKSGGEFFTPQEVSELLVELTLIDFNNENKDVRRKIGKVYDPC 60
Query: 313 SGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILH--GIQEFKI 370
GSG +L++ Y + E +K YGQE+N T L+++N+ LH G +F I
Sbjct: 61 CGSGSLLLK-YAKLNEGVK-----------FYGQEINLTTYNLARINMFLHNIGYDKFDI 108
Query: 371 FEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQ--DGYDETRLSDRRIKHIYKYGY 428
G D+L++P K N +D D +++NPP++ +G L++ H+ +
Sbjct: 109 KLG-DTLLDP------KHN--DDKPFDAIVSNPPYSTKWEGKSNPLLANDERFHVTQLA- 158
Query: 429 TSKQSADWAWVQLMLYYARRK--VGIVLDTGALFRGGAEKAIRQGIVED-DLIEAIILLP 485
K AD+A+V +L+ IV+ G L+R AE+ IR+ +V++ ++++++I LP
Sbjct: 159 -PKGKADFAFVLHILHNLSSSGTAAIVMFPGTLYRDHAEQDIRKYLVDNVNVVDSVIQLP 217
Query: 486 EKLFYNAAAPGIIMVLNPNKPE-ERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVD 544
+ LF+ + I+VL NK + ILF++AS+EF K N+L + +I+KIVD
Sbjct: 218 DNLFFGTSISTCIIVLRKNKNNNDNANGILFVDASKEFVKSGIK---NKLTNANIKKIVD 274
Query: 545 AYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKE----IEK 600
R KE+ FS++V+ EE++ +YNL+V+ YV ED E+ID+ + KE IEK
Sbjct: 275 TIRFKKEVTYFSKLVSREEVKNKNYNLSVNTYVEKEDTSEKIDIKLLNMQIKEIVAKIEK 334
Query: 601 RERELVE 607
+E+ E
Sbjct: 335 LRKEIDE 341
>pir||G64114 site-specific DNA-methyltransferase (adenine-specific) (EC
2.1.1.72) hsdM - Haemophilus influenzae (strain Rd KW20)
>gi|1574745|gb|AAC22936.1| (U32808) type I modification
enzyme (hsdM) [Haemophilus influenzae Rd]
Length = 443
Score = 159 bits (399), Expect = 7e-38
Identities = 116/373 (31%), Positives = 191/373 (51%), Gaps = 35/373 (9%)
Query: 263 DILGDAYEWILSYFAPQKAKE-GEVYTPREVIRLLVELLDIEDGSDILDPASGSGGMLIE 321
DILG YE+ LS FA + K G+ +TP+ ++ L+VE+L+ G + DPA GSGG ++
Sbjct: 94 DILGHVYEYFLSRFAQAEGKRSGQYFTPKSIVSLIVEMLEPYSGR-VYDPAMGSGGFFVQ 152
Query: 322 AYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQ 381
R++ + +YGQE N T L+ +N+ + GI ADS PQ
Sbjct: 153 TERFITAHQGNIN-----NVSIYGQEFNPTTWKLAAMNMAIRGIDYDFGKYNADSFTQPQ 207
Query: 382 WEEELKRNGIEDGKVDYVIANPPWN-QDGYDETRLSDRRIKHIYKYGYTSKQSADWAWVQ 440
D K+D+++ANP +N ++ ++E+ D R + YG K +A++AW+Q
Sbjct: 208 HI---------DKKMDFIMANPHFNDKEWWNESLADDPR----WAYGTPPKGNANFAWLQ 254
Query: 441 LMLYYA--RRKVGIVLDTGALF-RGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGI 497
M+Y+ K+ ++L G++ + E IR+ I+ DL+E ++ LP +LF N P
Sbjct: 255 HMIYHLSPNGKIALLLANGSMSSQTNNEGEIRKAIINADLVECMVALPGQLFTNTKIPAC 314
Query: 498 IMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYREFKEIEG--- 554
I LN NK +RKG++LFI+A + + + R L + I KI D ++ +G
Sbjct: 315 IWFLNRNK--KRKGEVLFIDARQ--IGYMKDRVLRDFTADDIAKIADTLHAWQTSDGYED 370
Query: 555 ---FSRVVTLEEIRKNDYNLNVSLYV-FPEDEREQIDLAKEFKEFKEIEKRERELVEKAK 610
F + TLEEI+ ND+ L YV E E + + A++ + + K + + +
Sbjct: 371 QAAFCKSATLEEIKNNDFVLTPGRYVGTAEQEDDGVPFAEKMQNLTALLKEQFAKSAELE 430
Query: 611 AYIEGILGAMDNE 623
A I+ LG + E
Sbjct: 431 AEIKKNLGGLGYE 443
>pir||C70880 probable hsdM protein - Mycobacterium tuberculosis (strain H37RV)
>gi|2624278|emb|CAA15552.1| (AL008967) hsdM
[Mycobacterium tuberculosis]
Length = 540
Score = 152 bits (380), Expect = 1e-35
Identities = 151/555 (27%), Positives = 257/555 (46%), Gaps = 82/555 (14%)
Query: 108 RLKFPTETKISRDRLISLLKAAADQIRGGLD---YK-ALLVFLFYKAISDRWMKMAQ--- 160
R K + + L L AAD++RG L YK +L +F K +SD + + +
Sbjct: 4 RKKQAPQAPSTMKELKDTLWKAADKLRGSLSASQYKDVILGLVFLKYVSDAYDERREAIR 63
Query: 161 -DLMKEGKTKTQAYILTN--KRY--YNLFDEDTGKLYTWHEVVKSRETIKEMANALIK-I 214
+L EG ++Q L + ++Y Y +F W + ++ + + K I
Sbjct: 64 AELAAEGMEESQIEDLIDDPEQYQGYGVFVVPVSA--RWKFLAENTKGKPAVGGEPAKNI 121
Query: 215 SEMNEELAD-LKKLVEVLG--LIGFIKEDNL--HKLEEIVKIFNRVDFAEFDS----DIL 265
++ +E D + K LG L +DN+ +L E++ +FN F+ D++
Sbjct: 122 GQLIDEAMDAVMKANPTLGGTLPRLYNKDNIDQRRLGELIDLFNSARFSRQGEHRARDLM 181
Query: 266 GDAYEWILSYFAPQKAKEG-EVYTPREVIRLLVELLDIEDGSDILDPASGSGGMLIEAYR 324
G+ YE+ L FA + K G E +TP V++++VE+L+ G + DP GSGGM ++ +
Sbjct: 182 GEVYEYFLGNFARAEGKRGGEFFTPPSVVKVIVEVLEPSSGR-VYDPCCGSGGMFVQTEK 240
Query: 325 YVKEKLKKEGFDEEPA-IMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWE 383
++ E D +P + +YGQE E T ++K+NL +HGI + + +W
Sbjct: 241 FIYEH------DGDPKDVSIYGQESIEETWRMAKMNLAIHGID--------NKGLGARWS 286
Query: 384 EELKRNGIEDGKVDYVIANPPWNQDGYDETRLSDRRIKHIYKYGYTSKQSADWAWVQLML 443
+ R+ D ++DYV+AN P+N + R +++G +A++AW+Q +L
Sbjct: 287 DTFARDQHPDVQMDYVMANLPFNIKDWARNEEDPR-----WRFGVPPANNANYAWIQHIL 341
Query: 444 YYARR--KVGIVLDTGALF-RGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMV 500
Y + G+V+ G++ E IR IVE DL+ ++ LP +LF + P +
Sbjct: 342 YKLAPGGRAGVVMANGSMSSNSNGEGDIRAQIVEADLVSCMVALPTQLFRSTGIPVCLWF 401
Query: 501 LNPNKPE------ERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYRE------ 548
+K +R G++LFI+A RE + R L +E I +I D +
Sbjct: 402 FAKDKAAGKQGSIDRCGQVLFIDA-RELGDLVD-RAERALTNEEIVRIGDTFHAWRGSKS 459
Query: 549 -------FKEIEGFSRVVTLEEIRKNDYNLNVSLYV---FPEDEREQID---------LA 589
++++ GF + TL EI+ DY L YV ED+ E ID L
Sbjct: 460 AAVKGIMYEDVPGFCKSATLAEIKATDYALTPGRYVGTPAVEDDGEPIDEKMARLSKALL 519
Query: 590 KEFKEFKEIEKRERE 604
+ F E +E+ RE
Sbjct: 520 EAFDESARLERVVRE 534
>pir||A82523 type I restriction-modification system DNA methylase XF2728
[imported] - Xylella fastidiosa (strain 9a5c)
>gi|9107964|gb|AAF85513.1|AE004079_4 (AE004079) type I
restriction-modification system DNA methylase [Xylella
fastidiosa]
Length = 525
Score = 152 bits (380), Expect = 1e-35
Identities = 113/372 (30%), Positives = 188/372 (50%), Gaps = 35/372 (9%)
Query: 257 FAEFDSDILGDAYEWILSYFAPQKAKEG-EVYTPREVIRLLVELLDI--EDGSDILDPAS 313
F + D+ GDAYE+++ +A K G E YTP+EV +LL ++ + + + + DPA
Sbjct: 177 FTDNTIDLFGDAYEYLMQMYASTAGKSGGEFYTPQEVSQLLTQITVVGKTEVNKVYDPAC 236
Query: 314 GSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGI--QEFKIF 371
GSG +L+ + + + GF YGQE+N T L ++N+ LH + ++F I
Sbjct: 237 GSGSLLLNFVKVLGHDQVRRGF--------YGQEINLTTYNLCRINMFLHNVNYEKFHIA 288
Query: 372 EGADSLVNP-QWEEELKRNGIEDGKVDYVIANPPWN--QDGYDETRLSDRRIKHIYKYGY 428
G D+L +P W++E + +++NPP++ DG D L +
Sbjct: 289 HG-DTLTDPAHWDDE---------PFEAIVSNPPYSIKWDG-DSNALLINDPRFAPPGIL 337
Query: 429 TSKQSADWAWVQLMLYY--ARRKVGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPE 486
K AD A+ +L + IV G L+RGGAE+ IRQ +++++ ++A+I LP
Sbjct: 338 APKSKADLAFTLHILSWLAVNGTAAIVEFPGVLYRGGAEQKIRQYLIDNNYVDAVIQLPA 397
Query: 487 KLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAY 546
LF+ I+VL +K R LF++AS + N+L H +KI+D +
Sbjct: 398 DLFFGTTIATCIIVLKKSK---RDNATLFMDASSLCVRSGTK---NKLTPAHQKKILDGF 451
Query: 547 REFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKEFKEIEKRERELV 606
++IE F+R+V +I N YN+ VS Y+ D RE ID+ ++ I R+ EL
Sbjct: 452 TARQDIEHFARLVDNSDIAANGYNIAVSSYIAHADTRESIDIKALNRDIARIVARQAELR 511
Query: 607 EKAKAYIEGILG 618
++ A + + G
Sbjct: 512 KQIDAIVADLEG 523
>pir||T30327 hsdM protein - Klebsiella pneumoniae >gi|2408224|gb|AAB70709.1|
(U93843) HsdM [Klebsiella pneumoniae]
Length = 539
Score = 134 bits (334), Expect = 3e-30
Identities = 143/555 (25%), Positives = 238/555 (42%), Gaps = 85/555 (15%)
Query: 110 KFPTETKISRDRLISLLKAAADQIRGGLD---YK-ALLVFLFYKAISDRWMKMAQDLMKE 165
K PT+ ++ L A+Q+RG ++ YK +L +F K ISD++ + + E
Sbjct: 3 KAPTKKAKAKKGFEDTLWDTANQLRGSVESSEYKHVVLSLVFLKFISDKFEARRKKMEDE 62
Query: 166 GK---TKTQAYILTNKRYYNLFDEDTGKLYTWHEVVKSRETIKEMANALIKISEMNEELA 222
G+ + + + + +Y L +E H K + + AL I + N L
Sbjct: 63 GQGDFLEMEVFYQQDNIFY-LPEEARWSFIKQH--AKQDDIAVRIDTALSTIEKRNPTLK 119
Query: 223 -------------DLKKLVEVLGLIGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDAY 269
+ KKL ++ I DN+ L + A D++G Y
Sbjct: 120 GALPDNYFSRQNLETKKLASLIDTI-----DNIETLAHETDVE-----ALSKEDLVGRVY 169
Query: 270 EWILSYFAPQKAKEG-EVYTPREVIRLLVELLDIEDGSDILDPASGSGGMLIEAYRYVKE 328
E+ L FA + K G E YTP+ V+ LL E+L+ +G I DP GS GM +++ ++V+
Sbjct: 170 EYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFEGK-IYDPCCGSAGMFVQSVKFVES 228
Query: 329 KLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEELKR 388
K I LYGQEL T L+K+NL + G+ AD+ + Q
Sbjct: 229 HQGKSR-----DIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQHP----- 278
Query: 389 NGIEDGKVDYVIANPPWN-QDGYDETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYAR 447
D K DY++ANPP+N +D ++ L+ Y+ T + W L A
Sbjct: 279 ----DLKADYILANPPFNLKDWRNDAELTKDPRFAGYRTPPTGNANYGWILHMLSKLSAN 334
Query: 448 RKVGIVLDTGALFRG-GAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKP 506
G VL G++ E IR ++E+DLI+ +I LP +LF+ P + + +K
Sbjct: 335 GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFFTTQIPVCLWFMTKSKA 394
Query: 507 EE-------RKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYR------------ 547
+ R+G+ LFI+A R + +L + I I D Y
Sbjct: 395 ADPAKGYRNRQGETLFIDARNLGTMIN--RTIKELTADDIAIIADTYHAWRSTPEELVER 452
Query: 548 ---------EFKEIEGFSRVVTLEEIRKNDYNLNVSLYV-FPEDEREQIDLAKEFKEFKE 597
++++ GF +V T+ E++ NDY L YV E E + + + +E +
Sbjct: 453 VKRGDSKLAQYEDQAGFCKVATIAEMKANDYVLTPGRYVGAAEQEDDGVAFETKMRELSQ 512
Query: 598 ---IEKRERELVEKA 609
+ ++ E ++KA
Sbjct: 513 TLFAQMKQAEELDKA 527
>emb|CAA68057.1| (X99719) Sty SBLI [Salmonella enterica]
Length = 539
Score = 130 bits (323), Expect = 5e-29
Identities = 141/541 (26%), Positives = 228/541 (42%), Gaps = 84/541 (15%)
Query: 110 KFPTETKISRDRLISLLKAAADQIRGGLD---YK-ALLVFLFYKAISDRWMKMAQDLMKE 165
K PT+ ++ L A+Q+RG ++ YK +L +F K ISD++ + + E
Sbjct: 3 KAPTKKAKAKKGFEDTLWDTANQLRGSVESSEYKHVVLSLVFLKFISDKFEARRKQMEDE 62
Query: 166 GKTKTQAYILTNKRYY---NLFDEDTGKLYTW-HEVVKSRETIKEMANALIKISEMNEEL 221
G Q L + +Y N+F +++ + K + + AL I + N L
Sbjct: 63 G----QGDFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAVRIDTALSTIEKRNPSL 118
Query: 222 A-------------DLKKLVEVLGLIGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDA 268
+ KKL ++ I DN+ L + A D++G
Sbjct: 119 KGALPDNYFSRQNLETKKLASLIDTI-----DNIETLAHETDVE-----ALSKEDLVGRV 168
Query: 269 YEWILSYFAPQKAKEG-EVYTPREVIRLLVELLDIEDGSDILDPASGSGGMLIEAYRYVK 327
YE+ L FA + K G E YTP+ V+ LL E+L+ G I DP GS GM +++ ++V+
Sbjct: 169 YEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGK-IYDPCCGSAGMFVQSVKFVE 227
Query: 328 EKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEELK 387
K I LYGQEL T L+K+NL + G+ AD+ + Q
Sbjct: 228 SHQGKSR-----DIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQHP---- 278
Query: 388 RNGIEDGKVDYVIANPPWN-QDGYDETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYA 446
D K DY++ANPP+N +D ++ L+ Y+ T + W L A
Sbjct: 279 -----DLKADYILANPPFNLKDWRNDAELTKDPRFAGYRTPPTGNANYGWILHMLSKLSA 333
Query: 447 RRKVGIVLDTGALFRG-GAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNK 505
G VL G++ E IR ++E+DLI+ +I LP +LF+ P + + +K
Sbjct: 334 NGTAGFVLANGSMSSNTSGEAEIRAQMIENDLIDCMIALPGQLFFTTQIPVCLWFMTKSK 393
Query: 506 PEE-------RKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYR----------- 547
+ R+G+ LFI+A K +L + I I D Y
Sbjct: 394 AADPAKGYRNRQGETLFIDARNLGTMINRTTK--ELTADDIATIADTYHAWRSTPEELVE 451
Query: 548 ----------EFKEIEGFSRVVTLEEIRKNDYNLNVSLYV-FPEDEREQIDLAKEFKEFK 596
++++ GF +V T+ EI+ ND L YV E E + + + +E
Sbjct: 452 RVKRGDSQLAQYEDQAGFCKVATIAEIKANDSVLTPGRYVGAAEQEDDGVAFETKMRELS 511
Query: 597 E 597
+
Sbjct: 512 Q 512
>pir||A82161 probable DNA methylase HsdM VC1769 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9656291|gb|AAF94918.1|
(AE004253) DNA methylase HsdM, putative [Vibrio
cholerae]
Length = 793
Score = 128 bits (319), Expect = 2e-28
Identities = 104/325 (32%), Positives = 161/325 (49%), Gaps = 50/325 (15%)
Query: 265 LGDAYEWILSYFAPQKAKE-GEVYTPREVIRLLVELL--DIEDGSD----ILDPASGSGG 317
+G +E ++ F + +E GE +TPREVI L+ L+ I+D + DPA GSGG
Sbjct: 177 MGYVFEELIRKFNEENNEEAGEHFTPREVIELMTHLVFDPIKDNLPLSITVYDPACGSGG 236
Query: 318 MLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADSL 377
ML E +V+EK D I LYG+E+N+ T A+ K ++++ G I G+
Sbjct: 237 MLTETQNFVEEKYPASNRD----IYLYGKEINDETYAICKSDMMIKGNNPENIRVGST-- 290
Query: 378 VNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETR-------LSDRRIKHIYK--YGY 428
L + + D++++NPP+ + E + + D R K K +G
Sbjct: 291 --------LSTDEFSSNRFDFMLSNPPYGKSWASEQKHIKEGKEVVDGRFKVKLKDYWGV 342
Query: 429 TSKQ-----SADWAWVQLMLYYARRK----------VGIVLDTGALFRGGA---EKAIRQ 470
S+Q S+D + LM + K + V + +LF G A E IR+
Sbjct: 343 ESEQEAIPRSSDGQLLFLMEMVTKMKSPQDSPLGSRIASVHNGSSLFTGDAGSGESNIRR 402
Query: 471 GIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPE--V 528
I+E+D+++AI+ LP LFYN I +LN NKPE R+GK+ I+AS FRK +
Sbjct: 403 FIIENDMLDAIVQLPNNLFYNTGITTYIWLLNNNKPENRQGKVQLIDASLLFRKLRKNLG 462
Query: 529 RKLNQLADEHIRKIVDAYREFKEIE 553
K + + EHI +IV Y E + +E
Sbjct: 463 NKNCEFSPEHIAEIVSTYLENQSVE 487
>emb|CAA09337.1| (AJ010745) hsdM-like gene [Klebsiella pneumoniae]
Length = 396
Score = 116 bits (289), Expect = 5e-25
Identities = 108/398 (27%), Positives = 195/398 (48%), Gaps = 57/398 (14%)
Query: 128 AAADQIRG---GLDYKA-LLVFLFYKAISDRWMKMAQ-----------------DLMKEG 166
A A+++RG G D+K +L LFY+ IS+ + + D +K+
Sbjct: 16 AIANEVRGAVDGWDFKQYVLGALFYRFISENFTSYIEAGDDSVQYAGMADSDIGDEIKDD 75
Query: 167 KTKTQAYILTNKRYY-NLFDEDTGKLYTWHEVVKSRETIKEMANALIKISEMNEELADLK 225
+T+ Y + + + N+ + + ++ I+ A+ +++ AD
Sbjct: 76 AVRTKGYFIAPSQLFCNVANGANTNDHLNADLNSIFVAIESSASGYPSEADIKGLFADFD 135
Query: 226 KLVEVLGLIGFIKEDNLHKLEEIVKIFNRVDFAEFDS---DILGDAYEWILSYFAPQKAK 282
LG +KE N+ +L ++K + +FD+ D+ GDAYE+++S +A K
Sbjct: 136 TTSNRLG--STVKEKNI-RLAAVLKGVEGLALGDFDAHQIDLFGDAYEFLISNYAANGGK 192
Query: 283 EG-EVYTPREVIRLLVELLDIEDGS--DILDPASGSGGMLIEAYRYVKEKLKKEGFDEEP 339
G E +TP+ V +L+ +L S I DPA+GSG +L++A ++ + ++GF
Sbjct: 193 SGGEFFTPQHVSKLIAQLAMHGQTSVNKIYDPAAGSGSLLLQAKKHFDNHIIEDGF---- 248
Query: 340 AIMLYGQELNEVTAALSKLNLILHGIQ--EFKIFEGADSLVNPQWEEELKRNGIEDGKVD 397
+GQE+N T L+++N+ LH I +F I G ++L+ P++++E D
Sbjct: 249 ----FGQEINHTTFNLARMNMFLHNINYDKFDIRLG-NTLLAPEFKDEKP--------FD 295
Query: 398 YVIANPPWNQDGY---DETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYY--ARRKVGI 452
+++NPP++ D T ++D R SK AD+A+V L Y A+ + I
Sbjct: 296 AIVSNPPYSVKWVGSDDPTLINDERFAPAGVLAPKSK--ADFAFVLHALNYLSAKGRAPI 353
Query: 453 VLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFY 490
V G +RGGAE+ IR+ +V+ + +E +I L LF+
Sbjct: 354 VCFPGIFYRGGAEQKIRKYLVDSNYVETVISLAPNLFF 391
>pir||F83304 probable restriction-modification system protein PA2735 [imported]
- Pseudomonas aeruginosa (strain PAO1)
>gi|9948812|gb|AAG06123.1|AE004701_6 (AE004701) probable
restriction-modification system protein [Pseudomonas
aeruginosa]
Length = 792
Score = 112 bits (277), Expect = 1e-23
Identities = 109/372 (29%), Positives = 180/372 (48%), Gaps = 70/372 (18%)
Query: 224 LKKLVEVLGLIGFIKED------NLHKLEEIVKIFNRVDFAEFDSDILGDAYEWILSYFA 277
++KLV+ ++G++ ED NL L + R D+ +G +E ++ F
Sbjct: 134 IQKLVDA-HILGYLIEDFLDPEVNLSPLP-VKDADGRTKLPALDNHGMGTVFEELIRRFN 191
Query: 278 PQKAKE-GEVYTPREVIRLLVELL------DIEDGS-DILDPASGSGGMLIEAYRYVKEK 329
+ +E GE +TPR+V++L+ +LL IE + + D + G+GGML A +KE
Sbjct: 192 EENNEEAGEHFTPRDVVQLMAKLLFLPVADRIESSTYSLYDGSCGTGGMLTVAEEALKEL 251
Query: 330 LKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGADS--LVNPQWEEELK 387
++ G D +I L+GQE+++ T A+ K +L+L G EGA++ +V + L
Sbjct: 252 AEQHGKDV--SIHLFGQEISDETYAICKADLLLKG-------EGAEAENIVGGADKSTLS 302
Query: 388 RNGIEDGKVDYVIANPPW------------------------NQDGYDE----TRLSDRR 419
+ + D++I+NPP+ N G E TR SD +
Sbjct: 303 ADQFRSREFDFMISNPPYGKSWKTDLERMGGKKEFSDPRFIVNHGGDAEFKLITRSSDGQ 362
Query: 420 IKHIYKYGYTSKQSADWAWVQLMLYYARRKVGIVLDTGALFRGGA---EKAIRQGIVEDD 476
+ +++ SK D A ++ +V + ALF G A E IR+ ++E+D
Sbjct: 363 L--MFQVNKLSKMKHDTA--------LGSRIALVHNGSALFTGDAGQGESNIRRWVLEND 412
Query: 477 LIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFR--KHPEVRKLNQL 534
+EAII LP +FYN I VL K E RKG++ I+AS+ F + +K +L
Sbjct: 413 WLEAIIALPLNIFYNTGIATYIWVLANKKAEHRKGRVQLIDASQWFAPLRRNLGKKNCEL 472
Query: 535 ADEHIRKIVDAY 546
A+ IR+I+D Y
Sbjct: 473 AEGDIRRILDLY 484
>gb|AAD47620.1| (AF153413) truncated HsdM [Lactococcus lactis]
Length = 206
Score = 105 bits (260), Expect = 1e-21
Identities = 72/214 (33%), Positives = 116/214 (53%), Gaps = 23/214 (10%)
Query: 358 LNLILHGIQEFKI-FEGADSLVNPQWEEELKRNGIEDGK-----VDYVIANPPWNQDGYD 411
+NL++H I+ I AD+L + W + G+ +GK D V+ANPP++ +
Sbjct: 1 MNLMMHNIEYNDIQIHHADTLES-DWPD-----GVIEGKDTPRMFDAVMANPPYSAHWNN 54
Query: 412 ETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARR--KVGIVLDTGALFRGGAEKAIR 469
+ R D R + +YG K AD++++ LY+ + +V I+L G LFRG AE IR
Sbjct: 55 KDREDDPRFR---EYGIAPKTKADYSFLLHCLYHTKESGRVAIILPHGVLFRGAAEGRIR 111
Query: 470 QGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVR 529
+ +++ IEA+I P+KLF N P +++L N+ ILF++AS+ F K ++
Sbjct: 112 KALIDKHQIEAVIGFPDKLFLNTGIPVCVLILKKNR---ANSDILFVDASQGFEK---MK 165
Query: 530 KLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEE 563
L QL E I KI + K ++ +S + TLEE
Sbjct: 166 NLKQLRPEDIDKITETVIHRKAVDKYSHLATLEE 199
>gb|AAG22013.1|AF288037_2 (AF288037) putative HsdM [Streptococcus thermophilus]
Length = 535
Score = 101 bits (249), Expect = 3e-20
Identities = 116/499 (23%), Positives = 224/499 (44%), Gaps = 66/499 (13%)
Query: 138 DYKALLVFLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKRYYNLFDEDTGKLYTWHEV 197
+YK L YK ++D+++ AQ++ + + + + ++ Y+ ED G W +
Sbjct: 31 EYKLLTQSFLYKFLNDKFLYEAQNV--DSENTYEHLVSMSEEDYDWLLEDIGTSTAWMKP 88
Query: 198 VKSRETI----------KEMANALIKISEMNEEL----ADLKKLVEVLG--LIGFIKEDN 241
+ ET+ + N L +I+ N ++ D + + LI I D+
Sbjct: 89 NQFIETLHRKQNESDFYETFENTLNQIAIDNNDIFSVHTDRNTAIRLFDERLITDIIPDS 148
Query: 242 LHKLEEIVKIFNRVDFAEFDSDILGDAYEW---ILSYFAPQKAKEG-----EVYTPREVI 293
+ E I N + +FD I +++ I Y K+G E YTP V
Sbjct: 149 SKRNEVAKSIINLLARVKFDEAIFSQGFDFFSTIFEYMIKDYNKDGGGTYAEYYTPHSVA 208
Query: 294 RLLVELLDIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTA 353
+++ ++L G+D G L+ + + G D+ +Y Q++++ ++
Sbjct: 209 KIIADILV---GNDNPQNVRIYGTHLLVWEPCLMNLASRIGVDKAT---VYSQDISQKSS 262
Query: 354 ALSKLNLILHGIQE--FKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYD 411
L + NLIL+G+Q I EG L N E K+DY+++NPP+ D +
Sbjct: 263 NLLRFNLILNGLQHSIHNIVEGNTILRNRHPE-----------KMDYIVSNPPFKLD-FS 310
Query: 412 ETR--------LSDRRIKHIYKYGYTSKQSADW--AWVQLMLYYARR--KVGIVLDTGAL 459
E R S+R + K + K S +VQ ++Y + + +VL TG +
Sbjct: 311 EWRDQVETLPEASERFFAGVPKTPKSKKNSMAIYELFVQHIIYSLKSDGQAAVVLPTGFI 370
Query: 460 F-RGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINA 518
+ G +K IRQ +V++ ++ ++ +P +F I+ ++ ++ KG ++ I+A
Sbjct: 371 TAQNGIDKKIRQHLVDNQMLAGVVSMPSNIFATTGTNVSILFID----KKNKGDVVLIDA 426
Query: 519 SREFRKHPEVRKLNQ-LADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYV 577
S K E + L+ E +KIV+ + + + +E FS V+ E+I++ +Y+L+ Y
Sbjct: 427 SNLGTKVKESKNQKTVLSPEEEQKIVETFIQKEAVEDFSVTVSYEDIKEKNYSLSAGQYF 486
Query: 578 FPEDEREQIDLAKEFKEFK 596
+ + + +D+ E E K
Sbjct: 487 --DIKIDYVDITAEDFEAK 503
>emb|CAA10699.1| (AJ132566) HsdM protein [Escherichia coli]
Length = 325
Score = 99.5 bits (244), Expect = 1e-19
Identities = 91/337 (27%), Positives = 143/337 (42%), Gaps = 51/337 (15%)
Query: 315 SGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGA 374
S GM +++ ++V+ K I LYGQEL T L+K+NL + G+ A
Sbjct: 1 SAGMFVQSVKFVESHQGKSR-----DIALYGQELTATTYKLAKMNLAIRGLSANLGERPA 55
Query: 375 DSLVNPQWEEELKRNGIEDGKVDYVIANPPWN-QDGYDETRLSDRRIKHIYKYGYTSKQS 433
D+ + Q D K DY++ANPP+N +D +E L+ Y+ T +
Sbjct: 56 DTFFSDQHP---------DLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNAN 106
Query: 434 ADWAWVQLMLYYARRKVGIVLDTGALFRG-GAEKAIRQGIVEDDLIEAIILLPEKLFYNA 492
W L A G VL G++ E IR ++E+DLI+ +I LP +LFY
Sbjct: 107 YGWILHMLSKLSANGTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTT 166
Query: 493 AAPGIIMVLNPNKP-------EERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDA 545
P + + +K +R+G+ LFI+A K +L E I I D
Sbjct: 167 QIPVCLWFMTKSKAADPAKGYRDRQGETLFIDARNLGTMISRTTK--ELTAEDIATIADT 224
Query: 546 Y---------------------REFKEIEGFSRVVTLEEIRKNDYNLNVSLYV-FPEDER 583
Y ++++ GF +V TL++I+ NDY L YV E E
Sbjct: 225 YHAWRSTPEELAARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEE 284
Query: 584 EQIDLAKEFKEFK----EIEKRERELVEKAKAYIEGI 616
+ + + +E E K+ EL + +EG+
Sbjct: 285 DGVAFETKMRELSKTLFEQMKQAEELDRAIRQNLEGL 321
>pir||T28670 hypothetical protein - Salmonella choleraesuis
>gi|1679865|emb|CAA68056.1| (X99719) orf2; similarity to
adenine methylase; putative [Salmonella enterica]
Length = 417
Score = 98.3 bits (241), Expect = 2e-19
Identities = 86/324 (26%), Positives = 154/324 (46%), Gaps = 58/324 (17%)
Query: 287 YTPREVIRLLVELLDIEDGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQ 346
YTP ++ + ++ + G DPA GSG ++ E +K + + + G
Sbjct: 134 YTPHGIVTFMGNIIQAQKGESFFDPACGSG-------EFISEIIKNQ-------VAISGS 179
Query: 347 ELNEVTAALSKLNLILHGIQEFKIFEGADSLVNPQWEEE---LKRNGIEDGKVDYVIANP 403
E + +SK+ ++++ + + S ++P + E LK+N D +++NP
Sbjct: 180 EYDVDRLKISKMKMLVNDL--------SPSNISPSYFTEGHNLKKN------FDIILSNP 225
Query: 404 PWNQDGYDETRLSDRRIKHIYKYGYTSKQSADWAWVQLMLYYARR--KVGIVLDTGALFR 461
P++ ++ H YG +AD+A++Q ++ + + I+L G LFR
Sbjct: 226 PFS------LKIPFDMEMHFCMYGKPPTSNADFAFLQYCIFMLKDNGRAAIILPDGILFR 279
Query: 462 GGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASRE 521
G E IR+ I++++ I AII LP+ +F A I+V K +++ IL IN
Sbjct: 280 EGKEYEIRKKIIKNNHISAIIYLPKGMFKTTAIATNIIVF---KKKQKTNDILMIN---- 332
Query: 522 FRKHPEVRKLNQLADEHIRKIVDAYREFKEIEGFSRVVTLEEIRKNDYNLNVSLYVFPED 581
VRK N L + +++ R EI SR+ +L EI +DYNL+ SLY P+
Sbjct: 333 ------VRKKNNLNVNLLLELI-TKRSTTEI---SRLTSLNEISAHDYNLSASLYFRPQV 382
Query: 582 EREQIDLAKEFKEFKEIEKREREL 605
++ DL + + KE+E++ L
Sbjct: 383 KK--TDLKQLIMKQKELEEKLHSL 404
>pir||A47200 EcoA system protein M - Escherichia coli >gi|146396|gb|AAA23984.1|
(L02505) restriction-modification enzyme type I M
subunit [Escherichia coli]
Length = 489
Score = 94.4 bits (231), Expect = 3e-18
Identities = 70/254 (27%), Positives = 125/254 (48%), Gaps = 31/254 (12%)
Query: 245 LEEIVKIFNRVDFAEF-DSDILGDAYEWILSYFAPQKAKEGEVYTPREVIRLLVELLDIE 303
L +++ N +DF + + GD YE IL GE YTPR V R +V+ +D +
Sbjct: 123 LRQVINKLNEIDFTSASERHLFGDIYEQILKDLQ-SAGNAGEFYTPRAVTRFMVDRVDPK 181
Query: 304 DGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEP-AIMLYGQELNEVTAALSKLNLIL 362
G I+DPA G+GG L A+ +VK K K D + ++G E ++ L+ N++L
Sbjct: 182 LGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLL 241
Query: 363 HGIQEFKIFEGADSLVNP--QWEEELKRNGIEDGKVDYVIANPPW---NQDGYDETRLSD 417
HGI+ ++L P W+E+L D ++ NPP+ +DG ++ ++
Sbjct: 242 HGIEVPVQIRHDNTLNKPLSSWDEQL----------DVIVTNPPFGGTEEDGIEKNFPAE 291
Query: 418 RRIKHIYKYGYTSKQSADWAWVQLMLYYARR--KVGIVLDTGALFRGGAEKAIRQGIVED 475
+ ++++AD ++QL++ + + +VL G LF G + I++ + E+
Sbjct: 292 MQ----------TRETAD-LFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIKKLLTEE 340
Query: 476 DLIEAIILLPEKLF 489
+ I+ LP +F
Sbjct: 341 CNLHTIVRLPNGVF 354
>emb|CAA71895.1| (Y11005) StySKI methylase [Salmonella enterica]
Length = 493
Score = 94.0 bits (230), Expect = 4e-18
Identities = 94/401 (23%), Positives = 177/401 (43%), Gaps = 68/401 (16%)
Query: 245 LEEIVKIFNRVDFAEF-DSDILGDAYEWILSYFAPQKAKEGEVYTPREVIRLLVELLDIE 303
L +++ N +DF + + GD YE IL GE YTPR V R +V+ +D +
Sbjct: 127 LRQVINKLNEIDFTSASERHLFGDIYEQILKDLQ-SAGNAGEFYTPRAVTRFMVDRVDPK 185
Query: 304 DGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEP-AIMLYGQELNEVTAALSKLNLIL 362
G I+DPA G+GG L A+ +VK K D + ++G E ++ L+ N++L
Sbjct: 186 LGESIMDPACGTGGFLACAFDHVKNNYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLL 245
Query: 363 HGIQEFKIFEGADSLVNP--QWEEELKRNGIEDGKVDYVIANPPW---NQDGYDETRLSD 417
HGI+ ++L P W+E+L D ++ NPP+ +DG ++ ++
Sbjct: 246 HGIEVPVQIRHDNTLNKPLSSWDEQL----------DVIVTNPPFGGTEEDGIEKNFPAE 295
Query: 418 RRIKHIYKYGYTSKQSADWAWVQLMLYYARR--KVGIVLDTGALFRGGAEKAIRQGIVED 475
+ ++++AD ++QL++ + + +VL G LF G + I++ + E+
Sbjct: 296 MQ----------TRETAD-LFLQLIIEVLAKNGRAAVVLPDGTLFGEGVKTKIKKLLTEE 344
Query: 476 DLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASRE-----FRKHPEVRK 530
+ I+ LP +F N K +LF + F +HP
Sbjct: 345 CNLHTIVRLPNGVF--------------NPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAG 390
Query: 531 LNQLADEHIRKIVDAYREF----KEIEGF-SRV-------VTLEEIRKNDYNLNVSLYVF 578
+ + K + E E +GF SRV V+++E+ ++NL++
Sbjct: 391 VKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDEVIARNFNLDI----- 445
Query: 579 PEDEREQIDLAKEFKEFKEIEKRERELVEKAKAYIEGILGA 619
++ + ++ + E +++E ++ + + ILGA
Sbjct: 446 -KNPHQVETVSHDPDELLAQYAKQQEAIQTLRHQLRDILGA 485
>sp|Q47282|T1ME_ECOLI TYPE I RESTRICTION ENZYME ECOEI M PROTEIN (M.ECOEI)
>gi|2121114|pir||I41293 EcoE type I restriction
modification enzyme M subunit - Escherichia coli
>gi|304897|gb|AAD15049.1| (L18759) EcoE type I
restriction modification enzyme M subunit [Escherichia
coli]
Length = 490
Score = 93.2 bits (228), Expect = 8e-18
Identities = 70/254 (27%), Positives = 124/254 (48%), Gaps = 31/254 (12%)
Query: 245 LEEIVKIFNRVDFAEF-DSDILGDAYEWILSYFAPQKAKEGEVYTPREVIRLLVELLDIE 303
L +++ N +DF+ + + GD YE IL GE YTPR V R +V +D +
Sbjct: 123 LRQVINKLNEIDFSSSQERHLFGDIYEQILRDLQ-SAGNAGEFYTPRAVTRFMVNRIDPK 181
Query: 304 DGSDILDPASGSGGMLIEAYRYVKEKLKKEGFDEEP-AIMLYGQELNEVTAALSKLNLIL 362
G I+DPA G+GG L A+ +VK+ K D + +YG E ++ L N++L
Sbjct: 182 LGESIMDPACGTGGFLACAFDHVKDNYVKTTEDHKTLQQQIYGVEKKQLPHLLCTTNMLL 241
Query: 363 HGIQEFKIFEGADSLVNP--QWEEELKRNGIEDGKVDYVIANPPW---NQDGYDETRLSD 417
HGI+ ++L P W+E+ VD ++ NPP+ +DG ++ ++
Sbjct: 242 HGIEVPVQIRHDNTLNKPLSSWDEQ----------VDVIVTNPPFGGTEEDGIEKNFPAE 291
Query: 418 RRIKHIYKYGYTSKQSADWAWVQLMLYYA--RRKVGIVLDTGALFRGGAEKAIRQGIVED 475
+ ++++AD ++QL++ + + +VL G LF G + I++ + E+
Sbjct: 292 MQ----------TRETAD-LFLQLIIEVLADKGRAAVVLPDGTLFGEGVKTKIKKLLTEE 340
Query: 476 DLIEAIILLPEKLF 489
+ I+ LP +F
Sbjct: 341 CNLHTIVRLPNGVF 354
>sp|P07989|T1M_SALPO TYPE I RESTRICTION ENZYME STYSPI M PROTEIN
>gi|154132|gb|AAA27143.1| (L02507)
restriction-modification enzyme type I M subunit
[Salmonella enterica]
Length = 529
Score = 84.3 bits (205), Expect = 4e-15
Identities = 96/377 (25%), Positives = 156/377 (40%), Gaps = 43/377 (11%)
Query: 192 YTWHEVVKSR---ETIKEMANALIKISEMNEELADLKKLVEVLGLIGFIKEDNLHKLEEI 248
Y W ++ KSR E ++ N L+ + AD KKLV+ + +L E+
Sbjct: 54 YRWDDL-KSRIGQEQLQFYRNLLVHLG------ADEKKLVQAVFQNVNTTITQPKQLTEL 106
Query: 249 VKIFNRVDFAEFDS----DILGDAYEWILSYFAPQ-KAKEGEVYTPREVIRLLVELLDIE 303
V + +D+ D D GD YE +L A + K+ G+ +TPR +I+ ++ LL +
Sbjct: 107 VSSMDSLDWYNGDHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLLKPQ 166
Query: 304 DGSDILDPASGSGGMLIEAYRYVK------EKLKKEGFDEEPAIMLYGQELNEVTAALSK 357
+ DPA+G+ G LIEA RYVK + L + D + G EL T L+
Sbjct: 167 PREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDAQDFQIKKAFVGLELVPGTRRLAL 226
Query: 358 LNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSD 417
+N +LH I+ GA L N L +G + D V NPP+ +
Sbjct: 227 MNCLLHDIEGNLDHGGAIRLGN-----TLGSDGENLPQADIVATNPPFGS-------AAG 274
Query: 418 RRIKHIYKYGYTSKQSADWAWVQLMLYYARRKVGIVLDTGALFRGGAEKAIRQGIVEDDL 477
I + + ++KQ + L R +V D LF GG IR+ +++
Sbjct: 275 TNITRTFVHPTSNKQLCFMQHIIETLPPGGRAAAVVPD-NVLFEGGKGTDIRRDLMDKCH 333
Query: 478 IEAIILLPEKLFYNAAAPGIIM------VLNPNKPEERKGKILFINASREFRKHPEVRKL 531
+ I+ LP +FY ++ V NPN+ + + + P K
Sbjct: 334 LHTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNM---PSFGKR 390
Query: 532 NQLADEHIRKIVDAYRE 548
++H++ Y E
Sbjct: 391 TPFTEQHLQPFETVYGE 407
>pir||H71935 type I restriction enzyme (modification chain) - Helicobacter
pylori (strain J99) >gi|4154948|gb|AAD05994.1|
(AE001475) TYPE I RESTRICTION ENZYME (MODIFICATION
SUBUNIT) [Helicobacter pylori J99]
Length = 543
Score = 83.1 bits (202), Expect = 8e-15
Identities = 119/546 (21%), Positives = 229/546 (41%), Gaps = 90/546 (16%)
Query: 98 DVYDFRRTYYRLKFPTET-----KISRDRLISLLKAAADQIRGGLDYKALLVFLFYKAIS 152
D ++F ++ KFP +T + + +D+ L Y LL +LF K
Sbjct: 50 DKFEF---FFEQKFPNKTIRDYKDFNEEEKEYFFLTLSDKKLPKLAYDELLNYLFEKHFY 106
Query: 153 DRWMKMAQDLMKEGKTKTQAYILTNKRYYNLFDEDTGKLYTWHEVVKSRETIKEMANALI 212
D + + D + + A + K DE T L+ E++ + N
Sbjct: 107 DNDLHLKLDAIFNRISSNNAELFNTKST----DETTIALF---------ESVSQYINEGS 153
Query: 213 KISEMNEELADLKKLVEVLGLIGFIKEDNLHKLEEIVKIFNRVDFAEFDSDILGDAYEWI 272
K + L D K N + + + + N+ + D +E++
Sbjct: 154 KRANFTRSLLDKLK--------------NFNFKQAFLNLQNQQGY-----DFFAPIFEYL 194
Query: 273 LS-YFAPQKAKEGEVYTPREVIRLLVELLDIEDGSD--ILDPASGSGGMLIEAYRYVKEK 329
L Y E YTP + ++ +LL + I DP++G+G +L+ +
Sbjct: 195 LKDYNNNSGGTYAEYYTPLSIASIIAKLLVNKPTQSVKIYDPSAGTGTLLMALAHQI--- 251
Query: 330 LKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEF--KIFEGADSLVNPQWEEELK 387
G D + LY Q++++ + + KLNLIL+ + EG ++L NP ++ K
Sbjct: 252 ----GTD---SCTLYAQDISQKSLRMLKLNLILNDLTHSLRHAIEG-NTLTNPYHSKDHK 303
Query: 388 RNGIEDGKVDYVIANPPWNQDGYDE-TRLSDRR------IKHIYKYGYTSKQSADWAWVQ 440
GK+D++++NPP+ D +E +S + + +I K SK + Q
Sbjct: 304 ------GKMDFIVSNPPFKLDFSNEHAEISQNKNDFFLGVPNIPKND-KSKMPIYTLFFQ 356
Query: 441 --LMLYYARRKVGIVLDTGALF-RGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAPGI 497
L + + K I++ TG + + G E I + +V++ L+ +I +P ++F N +
Sbjct: 357 HCLNMLSPKGKGAIIVPTGFISAKSGVENKIVRHLVDERLVYGVICMPSQVFANTGT-NV 415
Query: 498 IMVLNPNKPEERKGKILFINASREFRKHPEVR-KLNQLADEHIRKIVDAYREFKEIEGFS 556
++ P ++ ++ I+AS+ ++ E + K +L + I++ ++ F
Sbjct: 416 SIIFFQKTPSAKE--VILIDASKLGEEYTENKNKKTRLRPSDMDLILETFQNKAPKSDFC 473
Query: 557 RVVTLEEIRKNDYNLNVSLYVFPEDEREQIDLAKEFKE------------FKEIEKRERE 604
+V+ +EI + +Y+LN Y ED E I A EF+ F E + ++E
Sbjct: 474 ALVSFDEITEKNYSLNPGQYFTIEDTSETISQA-EFENLMQQYSSELASLFDESQNLQQE 532
Query: 605 LVEKAK 610
++E K
Sbjct: 533 ILETLK 538
>emb|CAA64185.1| (X94423) hypothetical protein [Staphylococcus aureus bacteriophage
phi-42]
Length = 639
Score = 81.1 bits (197), Expect = 3e-14
Identities = 72/276 (26%), Positives = 115/276 (41%), Gaps = 42/276 (15%)
Query: 261 DSDILGDAYEWILSYFAPQKAKEGEVYTPREVIRLLVELLDIEDGSDILDPASGSGGMLI 320
D DILG+ Y + Y G V TPR + L+ EL+DI +LDP GSGG LI
Sbjct: 307 DMDILGNFYGEFVKYGGNDGNSLGIVLTPRHITNLMCELIDINKNDYVLDPCCGSGGFLI 366
Query: 321 EAYRYVKEKLKKEGFDEEPAI-----MLYGQELNEVTAALSKLNLILHGIQEFKIFEGAD 375
A K+ E DEE L+G EL + ++ N+IL G +G
Sbjct: 367 AA----MNKMLHETEDEEKKTHIKQEQLHGIELQQKLFTIATTNMILRG-------DGKS 415
Query: 376 SLVNP---QWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSDRRIKHIYKYGYTSKQ 432
+L E+EL N K+ + NPP++Q + + H+ + + ++
Sbjct: 416 NLKRDDIFHIEKELYAN-----KITKALINPPYSQ-------AKTKNLSHLSEISFINE- 462
Query: 433 SADWAWVQLMLYYARRKVGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNA 492
L L K+ ++ + ++ I+E+ ++ +I L + FY
Sbjct: 463 -------TLSLMKIGAKLAAIVPQSTMIGKTKNDNYKRDILENHSLDTVITLNKDTFYGV 515
Query: 493 AAPGIIMVLNPNKPEERKGKILFINASRE---FRKH 525
I V P++ K ++ FIN S + RKH
Sbjct: 516 GVNPCIAVFTAGIPQDEKKRVNFINFSDDGYIVRKH 551
>sp|P08957|T1MK_ECOLI TYPE I RESTRICTION ENZYME ECOKI M PROTEIN (M.ECOKI)
>gi|66464|pir||XYECHM site-specific
DNA-methyltransferase (adenine-specific) (EC 2.1.1.72)
EcoK - Escherichia coli >gi|41753|emb|CAA29792.1|
(X06545) hsdM gene product (AA 1 - 529) [Escherichia
coli] >gi|537191|gb|AAA97246.1| (U14003) CG Site No.
621; alternate gene names hs, hsm, hsp, rm [Escherichia
coli] >gi|1790808|gb|AAC77305.1| (AE000505) host
modification; DNA methylase M [Escherichia coli K12]
Length = 529
Score = 79.2 bits (192), Expect = 1e-13
Identities = 78/299 (26%), Positives = 124/299 (41%), Gaps = 29/299 (9%)
Query: 263 DILGDAYEWILSYFAPQ-KAKEGEVYTPREVIRLLVELLDIEDGSDILDPASGSGGMLIE 321
D GD YE +L A + K+ G+ +TPR +I+ ++ LL + + DPA+G+ G LIE
Sbjct: 125 DDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLLKPQPREVVQDPAAGTAGFLIE 184
Query: 322 AYRYVK------EKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQEFKIFEGAD 375
A RYVK + L + D + G EL T L+ +N +LH I+ GA
Sbjct: 185 ADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELVPGTRRLALMNCLLHDIEGNLDHGGAI 244
Query: 376 SLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSDRRIKHIYKYGYTSKQSAD 435
L N L +G K V NPP+ + I + + ++KQ
Sbjct: 245 RLGN-----TLGSDGENLPKAHIVATNPPFGS-------AAGTNITRTFVHPTSNKQLCF 292
Query: 436 WAWVQLMLYYARRKVGIVLDTGALFRGGAEKAIRQGIVEDDLIEAIILLPEKLFYNAAAP 495
+ L+ R +V+ LF GG IR+ +++ + I+ LP +FY
Sbjct: 293 MQHIIETLHPGGR-AAVVVPDNVLFEGGKGTDIRRDLMDKCHLHTILRLPTGIFYAQGVK 351
Query: 496 GIIM------VLNPNKPEERKGKILFINASREFRKHPEVRKLNQLADEHIRKIVDAYRE 548
++ V NPN+ + + + P K DEH++ Y E
Sbjct: 352 TNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNM---PSFGKRTPFTDEHLQPFERVYGE 407
>gb|AAA23985.1| (L02508) restriction-modification enzyme type I M subunit
[Escherichia coli]
Length = 529
Score = 78.8 bits (191), Expect = 2e-13
Identities = 94/377 (24%), Positives = 155/377 (40%), Gaps = 43/377 (11%)
Query: 192 YTWHEVVKSR---ETIKEMANALIKISEMNEELADLKKLVEVLGLIGFIKEDNLHKLEEI 248
Y W ++ KSR E ++ N L+ + AD +KLV+ + +L E+
Sbjct: 54 YRWDDL-KSRIGQEQLQFYRNLLVHLG------ADNQKLVQAVFQNVNTTITQPKQLTEL 106
Query: 249 VKIFNRVDFAEF----DSDILGDAYEWILSYFAPQ-KAKEGEVYTPREVIRLLVELLDIE 303
V + +D+ D GD YE +L A + K+ G+ +TPR +I+ ++ LL +
Sbjct: 107 VSNMDSLDWYNGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLLKPQ 166
Query: 304 DGSDILDPASGSGGMLIEAYRYVK------EKLKKEGFDEEPAIMLYGQELNEVTAALSK 357
+ DPA+G+ G LIEA RYVK + L + D + G EL T L+
Sbjct: 167 PREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELVPGTRRLAL 226
Query: 358 LNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSD 417
+N +LH I+ GA L N L +G K V NPP+ +
Sbjct: 227 MNCLLHDIEGNLDHGGAIRLGN-----TLGSDGENLPKAHIVATNPPFGS-------AAG 274
Query: 418 RRIKHIYKYGYTSKQSADWAWVQLMLYYARRKVGIVLDTGALFRGGAEKAIRQGIVEDDL 477
I + + ++KQ + L+ R +V+ LF GG IR+ +++
Sbjct: 275 TNITRTFVHPTSNKQLCFMQHIIETLHPGGR-AAVVVPDNVLFEGGKGTDIRRDLMDKCH 333
Query: 478 IEAIILLPEKLFYNAAAPGIIM------VLNPNKPEERKGKILFINASREFRKHPEVRKL 531
+ I+ LP +FY ++ V NP++ + + + P K
Sbjct: 334 LHTILRLPTGIFYAQGVKTNVLFFTKGTVANPHQDKNCTDDVWVYDLRTNM---PSFGKR 390
Query: 532 NQLADEHIRKIVDAYRE 548
DEH++ Y E
Sbjct: 391 TPFTDEHLQPFERVYGE 407
>sp|P40813|T1M_SALTY TYPE I RESTRICTION ENZYME STYSJI M PROTEIN
>gi|154130|gb|AAA19429.1| (L02506)
restriction-modification enzyme type I M subunit
[Salmonella enterica]
Length = 529
Score = 78.4 bits (190), Expect = 2e-13
Identities = 91/377 (24%), Positives = 158/377 (41%), Gaps = 43/377 (11%)
Query: 192 YTWHEVVKSR---ETIKEMANALIKISEMNEELADLKKLVEVLGLIGFIKEDNLHKLEEI 248
Y W ++ KSR + ++ N L+++ +D KKLV+ + + +L E+
Sbjct: 54 YRWDDL-KSRIGQDQMQFYRNLLVQLG------SDEKKLVQAVFHNVSTTIEQPKQLTEL 106
Query: 249 VKIFNRVDFAEFDS----DILGDAYEWILSYFAPQ-KAKEGEVYTPREVIRLLVELLDIE 303
V + +D+ + D GD YE +L A + K+ G+ +TPR +I+ ++ LL +
Sbjct: 107 VSYMDALDWYNGNHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTIIHLLKPQ 166
Query: 304 DGSDILDPASGSGGMLIEAYRYVK------EKLKKEGFDEEPAIMLYGQELNEVTAALSK 357
+ DPA+G+ G LIEA RYVK + L + D + G EL T L+
Sbjct: 167 PREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELVPGTRRLAL 226
Query: 358 LNLILHGIQEFKIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDETRLSD 417
+N +LH I+ GA L N L +G K V NPP+ +
Sbjct: 227 MNCLLHDIEGNLDHGGAIRLGN-----TLGSDGENLPKAHIVATNPPFGS-------AAG 274
Query: 418 RRIKHIYKYGYTSKQSADWAWVQLMLYYARRKVGIVLDTGALFRGGAEKAIRQGIVEDDL 477
I + + ++KQ + L+ R +V+ LF GG IR+ +++
Sbjct: 275 TNITRTFVHPTSNKQLCFMQHIIETLHPGGR-AAVVVPDNVLFEGGKGTDIRRDLMDKCH 333
Query: 478 IEAIILLPEKLFYNAAAPGIIM------VLNPNKPEERKGKILFINASREFRKHPEVRKL 531
+ I+ LP +FY ++ V NP++ + + + P K
Sbjct: 334 LHTILRLPTGIFYAQGVKTNVLFFTKGTVTNPHQDKNCTDDVWVYDLRTNM---PSFGKR 390
Query: 532 NQLADEHIRKIVDAYRE 548
++H++ Y E
Sbjct: 391 TPFTEQHLQPFETVYGE 407
>pir||G64577 type I restriction enzyme M protein - Helicobacter pylori (strain
26695) >gi|2313567|gb|AAD07525.1| (AE000561) type I
restriction enzyme M protein (hsdM) [Helicobacter pylori
26695]
Length = 487
Score = 73.4 bits (177), Expect = 7e-12
Identities = 101/451 (22%), Positives = 201/451 (44%), Gaps = 54/451 (11%)
Query: 145 FLFYKAISDRWMKMAQDLMKEGKTKTQAYILTNKRYYNLFDEDTGKLYTWHEVVKSRETI 204
FLF + ++ ++ +D KE K + LT+KR L +D Y + + +
Sbjct: 54 FLFEQEFPNQTIQDYKDFNKEEKEEF-FITLTDKRLPKLAYDDLLN-YLFEKHFNDNDLH 111
Query: 205 KEMANALIKISEMNEEL-----ADLKKLVEVLGLIGFIKEDNLHK------LEEIVKIFN 253
++ +IS N EL D + + +I E++ L+++ K
Sbjct: 112 LKLDIIFNRISSNNAELFNTTSTDKTTIALFESISQYINEESKRANFTRVLLDKLKKFNF 171
Query: 254 RVDFAEFDS----DILGDAYEWILS-YFAPQKAKEGEVYTPREVIRLLVELLDIEDGSD- 307
+ F + D +E++L Y K E YTP + ++ +LL E +
Sbjct: 172 KQAFLNLQNQQGYDFFAPIFEYLLKDYNNAGGGKYAEYYTPLSIASIIAKLLINEPTRNV 231
Query: 308 -ILDPASGSGGMLIEAYRYVKEKLKKEGFDEEPAIMLYGQELNEVTAALSKLNLILHGIQ 366
I DP++G+G +L+ + G D + LY Q++++ + + KLNLIL+ +
Sbjct: 232 KIYDPSAGTGTLLMALAHQI-------GTD---SCTLYAQDISQKSLRMLKLNLILNDLT 281
Query: 367 EF--KIFEGADSLVNPQWEEELKRNGIEDGKVDYVIANPPWNQDGYDE-TRLSDRR---- 419
EG ++L NP ++ K GK+DY+++NPP+ D +E +S +
Sbjct: 282 HSLRNAIEG-NTLTNPYHSKDFK------GKMDYIVSNPPFKLDFSNEHAEISQNKNDFF 334
Query: 420 --IKHIYKYGYTSKQSADWAWVQ--LMLYYARRKVGIVLDTGAL-FRGGAEKAIRQGIVE 474
+ +I K SK + Q L + + K I++ TG + + G E I + +V+
Sbjct: 335 LGVPNIPK-NNKSKMPIYTLFFQHCLNMLSNKGKGAIIVPTGFISAKSGVENKIIRHLVD 393
Query: 475 DDLIEAIILLPEKLFYNAAAPGIIMVLNPNKPEERKGKILFINASREFRKHPEVR-KLNQ 533
+ L+ ++ +P ++F N + ++ P + +++ I+AS+ ++ E + K +
Sbjct: 394 ERLVYGVVCMPSQVFANTGT-NVSIIFFQKTPSAK--EVVLIDASKLGEEYTENKNKKTR 450
Query: 534 LADEHIRKIVDAYREFKEIEGFSRVVTLEEI 564
L I I++ ++ + F +V+ +EI
Sbjct: 451 LRPSDIDLILETFQNKTKKSDFCALVSFDEI 481
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.318 0.138 0.391
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 232925451
Number of Sequences: 2977
Number of extensions: 10297335
Number of successful extensions: 38023
Number of sequences better than 1.0e-10: 50
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 45
Number of HSP's that attempted gapping in prelim test: 37791
Number of HSP's gapped (non-prelim): 57
length of query: 623
length of database: 189,106,746
effective HSP length: 59
effective length of query: 564
effective length of database: 153,796,013
effective search space: 86740951332
effective search space used: 86740951332
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 168 (69.9 bits)