BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2150 (hsdS) DE:type I restriction-modification enzyme, S
subunit
(427 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75221 type I restriction-modification enzyme, s chain PAB2... 848 0.0
pir||B64316 restriction modification system S chain homolog - Me... 194 2e-48
gi|11499300 type I restriction-modification enzyme, S subunit [A... 177 2e-43
sp|Q58926|YF31_METJA HYPOTHETICAL PROTEIN MJ1531 >gi|2129334|pir... 174 2e-42
sp|Q58615|YC18_METJA HYPOTHETICAL PROTEIN MJ1218 >gi|2129338|pir... 162 5e-39
pir||B69226 type I restriction modification system, subunit S - ... 144 2e-33
pir||C82522 type I restriction-modification system specificity d... 141 2e-32
sp|Q57594|Y130_METJA HYPOTHETICAL PROTEIN MJ0130 >gi|2826248|gb|... 132 5e-30
gb|AAG18734.1| (AE004978) type I restriction modification enzyme... 132 5e-30
sp|P06187|T1S_SALTY TYPE I RESTRICTION ENZYME STYSJI SPECIFICITY... 116 6e-25
gi|10956235 HsdS [Lactococcus lactis subsp. lactis] >gi|9294803|... 108 1e-22
sp|P06991|T1SD_ECOLI TYPE I RESTRICTION ENZYME ECODI SPECIFICITY... 103 3e-21
pir||E83304 hypothetical protein PA2734 [imported] - Pseudomonas... 100 4e-20
pir||NDECAS type I site-specific deoxyribonuclease (EC 3.1.21.3)... 92 1e-17
sp|P19704|T1SA_ECOLI TYPE I RESTRICTION ENZYME ECOAI SPECIFICITY... 92 1e-17
pir||H81307 restriction modification enzyme Cj1051c [imported] -... 88 2e-16
gb|AAG22014.1|AF288037_3 (AF288037) putative HsdS [Streptococcus... 85 2e-15
gi|10954528 M. jannaschii predicted coding region MJECL41 [Metha... 82 2e-14
sp|P19705|T1SE_ECOLI TYPE I RESTRICTION ENZYME ECOEI SPECIFICITY... 82 2e-14
pir||H82160 conserved hypothetical protein VC1768 [imported] - V... 81 3e-14
gb|AAF13313.1|AF109691_6 (AF109691) type I S-subunit [Lactobacil... 78 1e-13
gb|AAC64909.1| (AF027167) putative type I S-subunit protein [Str... 75 2e-12
pir||JC5218 type I site-specific deoxyribonuclease (EC 3.1.21.3)... 73 6e-12
gb|AAF69139.1|AF228680_3 (AF228680) HsdS [Lactococcus lactis] >g... 70 5e-11
>pir||D75221 type I restriction-modification enzyme, s chain PAB2150 -
Pyrococcus abyssi (strain Orsay)
>gi|5457728|emb|CAB49219.1| (AJ248283) type I
restriction-modification enzyme, S subunit [Pyrococcus
abyssi]
Length = 427
Score = 848 bits (2166), Expect = 0.0
Identities = 427/427 (100%), Positives = 427/427 (100%)
Query: 1 MSEASFTFYRETRFKEVELNGRKARIPEEWEVVELGEVARIRKKKSVRDIAEVAVIPMEK 60
MSEASFTFYRETRFKEVELNGRKARIPEEWEVVELGEVARIRKKKSVRDIAEVAVIPMEK
Sbjct: 1 MSEASFTFYRETRFKEVELNGRKARIPEEWEVVELGEVARIRKKKSVRDIAEVAVIPMEK 60
Query: 61 VPQDNELFAEFEIKAIEDVKSSTYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATT 120
VPQDNELFAEFEIKAIEDVKSSTYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATT
Sbjct: 61 VPQDNELFAEFEIKAIEDVKSSTYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATT 120
Query: 121 EVYPIVPSENLDVFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQ 180
EVYPIVPSENLDVFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQ
Sbjct: 121 EVYPIVPSENLDVFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQ 180
Query: 181 KKIAEILRSIDEAIQAVDESIARLERLKKGTMERLLTRGINHTRFKTVELNGRKVEIPEE 240
KKIAEILRSIDEAIQAVDESIARLERLKKGTMERLLTRGINHTRFKTVELNGRKVEIPEE
Sbjct: 181 KKIAEILRSIDEAIQAVDESIARLERLKKGTMERLLTRGINHTRFKTVELNGRKVEIPEE 240
Query: 241 WDVVELGEVAERRNESVNPANMGNIPFVGLEHIEPGNIRLSQWGNSSEVKSSKSKFYPGD 300
WDVVELGEVAERRNESVNPANMGNIPFVGLEHIEPGNIRLSQWGNSSEVKSSKSKFYPGD
Sbjct: 241 WDVVELGEVAERRNESVNPANMGNIPFVGLEHIEPGNIRLSQWGNSSEVKSSKSKFYPGD 300
Query: 301 ILYGKLRPYLDKAVIADFEGICSTDIIVIKAKEDKTIPEYLIWVIHSKEFIEYAKKTMKG 360
ILYGKLRPYLDKAVIADFEGICSTDIIVIKAKEDKTIPEYLIWVIHSKEFIEYAKKTMKG
Sbjct: 301 ILYGKLRPYLDKAVIADFEGICSTDIIVIKAKEDKTIPEYLIWVIHSKEFIEYAKKTMKG 360
Query: 361 VNHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERMKKAVMEKLL 420
VNHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERMKKAVMEKLL
Sbjct: 361 VNHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERMKKAVMEKLL 420
Query: 421 TGEVRVR 427
TGEVRVR
Sbjct: 421 TGEVRVR 427
>pir||B64316 restriction modification system S chain homolog - Methanococcus
jannaschii
Length = 425
Score = 194 bits (487), Expect = 2e-48
Identities = 152/446 (34%), Positives = 237/446 (53%), Gaps = 49/446 (10%)
Query: 8 FYRETRFKEVELNGRKARIPEEWEVVELGEVARIRK-----KKSVRDIAEVAVIP---ME 59
FY+E FK+ E+ IPE+WE+VEL +V + K K SV + + IP +E
Sbjct: 2 FYKEENFKKTEIG----EIPEDWEIVELKDVCKKIKAGGTPKTSVEEYYKNGTIPFVKIE 57
Query: 60 KVPQDNELFAEFEIKAIEDVKSSTYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALAT 119
+ N+ +IK E+ +++ ++ K + F G + N +AT
Sbjct: 58 DITNSNKYLTNTKIKITEEGLNNSNA----WIVPKNSVLF--AMYGSIGETAINKIEVAT 111
Query: 120 TE-VYPIVPSEN-LDVFFLFYIL-KDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPP 176
+ + I+P +N L+ FL+YIL K+K + L ++ T ++ K+ +IPLPP
Sbjct: 112 NQAILGIIPKDNILESEFLYYILAKNKNYYSKLGMQTTQKNLNAQIVKS----FKIPLPP 167
Query: 177 LEEQKKIAEILRSIDEAIQAVDESIARLERLKKGTMERLLTRGINHTRFKTVELNGRKVE 236
LEEQK+IA+IL IDE I+ +++SI +LER+KKG M +LLT+GI H+RFK E+ E
Sbjct: 168 LEEQKQIAKILTKIDEGIEIIEKSINKLERIKKGLMHKLLTKGIGHSRFKKSEIG----E 223
Query: 237 IPEEWDVVELGEVAERRNESVNPANMGNIPFVG-LEHIEPGNI-RLSQ--WGNSSEVKSS 292
IPE+W+V E+ ++ E + + G + I P ++ RL++ + SSE K +
Sbjct: 224 IPEDWEVFEIKDIFEVKTGTTPSTKKSEYWENGEINWITPLDLSRLNEKIYIGSSERKVT 283
Query: 293 K--------SKFYPGDILYGKLRPYLDKAVI---ADFEGICSTDIIVIKAKEDKTIPEYL 341
K + G I+ P AV+ + F C +V K + Y
Sbjct: 284 KIALEKCNLNLIPKGSIIISTRAPVGYVAVLTVESTFNQGCKG--LVPKNNDSVNTEFYA 341
Query: 342 IWVIHSKEFIEYAKKTMKGVNHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAK 401
++ K +E G S ++ F+IPLPPLEEQK+IA+IL ++D++IE K
Sbjct: 342 YYLKFKKNLLE---NLSGGSTFKELSKSMLENFKIPLPPLEEQKQIAKILSSVDKSIELK 398
Query: 402 RAKKEKLERMKKAVMEKLLTGEVRVR 427
+ KKEKL+RMKK +ME LLTG+VRV+
Sbjct: 399 KQKKEKLQRMKKKIMELLLTGKVRVK 424
>gi|11499300 type I restriction-modification enzyme, S subunit [Archaeoglobus
fulgidus] >gi|7448990|pir||E69463 type I
restriction-modification enzyme, S subunit homolog -
Archaeoglobus fulgidus >gi|2648839|gb|AAB89535.1|
(AE000985) type I restriction-modification enzyme, S
subunit [Archaeoglobus fulgidus]
Length = 341
Score = 177 bits (444), Expect = 2e-43
Identities = 121/355 (34%), Positives = 197/355 (55%), Gaps = 36/355 (10%)
Query: 82 STYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATTEVYPIVPSENLDVFFLFYILK 141
ST G + + +F G +G++P + SE F+ +Y+L
Sbjct: 9 STRAPVGYVAIVDEDTTFNQGCKGLIPKS-----------------SEINTEFYCYYLLL 51
Query: 142 DKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKIAEILRSIDEAIQAVDESI 201
KR + +++G + + + K L +L IPL PL EQ+KIAEIL ++D+AI+ VDE+I
Sbjct: 52 IKRKLE----QLSGGSTFKELPKKSLEELLIPLLPLPEQQKIAEILSTVDKAIEKVDEAI 107
Query: 202 ARLERLKKGTMERLLTRGINHTRFKTVELNGRKVEIPEEWDVVELGEVAE--RRNESVNP 259
A+ ERLKKG M+ LLT+GI H FK E+ IP+EW+VV LG+V E + SV
Sbjct: 108 AKTERLKKGLMQELLTKGIGHKEFKDTEIG----RIPKEWEVVRLGDVLELCQYGLSVPL 163
Query: 260 ANMGNIPFVGLEHIEPGNI--RLSQWGNSSEVKSSKSKFYPGDILYGKLR--PYLDKAVI 315
+ G P + ++ I G + ++++ + E K GD+L+ + + + I
Sbjct: 164 KDKGKYPVIRMDEIVNGYVVTDIAKYADLDEETFKNFKLEKGDVLFNRTNSLELVGRTGI 223
Query: 316 ADFEG--ICSTDIIVIKAKEDKTIPEYL-IWVIHSKEFIEYAKKTMKGVNHPRTSWKSIK 372
+G + ++ +I ++ K + P +L ++I S+ ++ + V+ + ++K
Sbjct: 224 FLLDGYYVFASYLIRLRPKHEILHPHFLTFYLIFSQSRLK--QLATVAVHQANINATNLK 281
Query: 373 QFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERMKKAVMEKLLTGEVRVR 427
+F+IPLPPL EQ+KIAEIL T+D+ +E +R +KEKLER+KK +M LLTG RVR
Sbjct: 282 KFKIPLPPLPEQQKIAEILSTVDKKLELERKRKEKLERIKKGLMNDLLTGRRRVR 336
Score = 82.7 bits (201), Expect = 7e-15
Identities = 68/225 (30%), Positives = 111/225 (49%), Gaps = 23/225 (10%)
Query: 14 FKEVELNGRKARIPEEWEVVELGEVARIRK---KKSVRDIAEVAVIPMEKVPQDNELFAE 70
FK+ E+ RIP+EWEVV LG+V + + ++D + VI M+++ +
Sbjct: 131 FKDTEIG----RIPKEWEVVRLGDVLELCQYGLSVPLKDKGKYPVIRMDEIVNG---YVV 183
Query: 71 FEIKAIEDVKSSTY----CEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATTEVYPIV 126
+I D+ T+ E GD+L + G+ GI + +G+ + + + +
Sbjct: 184 TDIAKYADLDEETFKNFKLEKGDVLFNRTNSLELVGRTGIF---LLDGYYVFASYLIRLR 240
Query: 127 PSENL--DVFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKIA 184
P + F FY++ + K L T + + T+L K +IPLPPL EQ+KIA
Sbjct: 241 PKHEILHPHFLTFYLIFSQSRLKQL---ATVAVHQANINATNLKKFKIPLPPLPEQQKIA 297
Query: 185 EILRSIDEAIQAVDESIARLERLKKGTMERLLTRGINHTRFKTVE 229
EIL ++D+ ++ + +LER+KKG M LLT G R K E
Sbjct: 298 EILSTVDKKLELERKRKEKLERIKKGLMNDLLT-GRRRVRVKANE 341
>sp|Q58926|YF31_METJA HYPOTHETICAL PROTEIN MJ1531 >gi|2129334|pir||B64491 type I
restriction enzyme CfrI specificity subunit homolog -
Methanococcus jannaschii >gi|1592162|gb|AAB99552.1|
(U67594) type I restriction-modification enzyme, S
subunit, putative [Methanococcus jannaschii]
Length = 425
Score = 174 bits (436), Expect = 2e-42
Identities = 133/443 (30%), Positives = 217/443 (48%), Gaps = 48/443 (10%)
Query: 8 FYRETRFKEVELNGRKARIPEEWEVVELGEVAR-IRKKKSVRDIAEVAVIPMEKVPQDNE 66
FY+E FK+ E+ IPE+WEV EL ++ IR + + + P+ ++ ++
Sbjct: 7 FYKEENFKKTEIG----EIPEDWEVRELKDILEVIRNGLTAKQNKDKIGYPITRIETISD 62
Query: 67 LFAEF-EIKAIEDVKSSTYCE----AGDLLLAKITPSFENGKQGIVPFNVPNGFALATTE 121
+ ++ +ED+K + GD+L + I GK I + F L
Sbjct: 63 SKIDITKLGYVEDIKQEDIAKYRLIIGDILFSHINSEEHIGKVAI--YEGKPEFLLHGMN 120
Query: 122 VYPIVPSEN-LDVFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQ 180
+ + P++N ++ ++L Y+L+ + + I + + + +T L L+IPLPPLEEQ
Sbjct: 121 LLLLRPNKNKIEPYYLLYLLRHFKQKNIFKYIAKRAVNQSSINQTQLKHLKIPLPPLEEQ 180
Query: 181 KKIAEILRSIDEAIQAVDESIARLERLKKGTMERLLTRGI-NHTRFKTVELNGRKVEIPE 239
K+IA+IL D I +++ I L + KKG M++L T+G+ H FK E+ EIPE
Sbjct: 181 KQIAKILSDFDNLIGTINKQIEVLNKAKKGMMKKLFTKGVFEHKSFKKSEIG----EIPE 236
Query: 240 EWDVVELGEVAERRNESVNPANMGNIPFVGLEHIEPGNIRLSQWGNSSEVKSSKSKFYPG 299
+W+VVELG NE MG P + E + Q K+ YP
Sbjct: 237 DWEVVELG------NEKYFKIIMGQSPPSSSYNKEGEGVPFLQG------KAEFGNIYPN 284
Query: 300 DILYGK--------------LRPYLDKAVIADFEGICSTDIIVIKAKEDKTIPEYLIWVI 345
+LY +R + IA F+ + IK+ ++K ++ + +
Sbjct: 285 PVLYTNKPLKVVDDEDILISVRAPVGDVNIAPFKLCIGRGLAGIKSNKEKVDNFFVFYYL 344
Query: 346 -HSKEFIEYAKKTMKGVNHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAK 404
+ K IEY G + K ++ +IPLPPLEEQK IA+ L+ ID+ IE KR +
Sbjct: 345 SYIKPKIEYLGG---GAVFKAITKKDLESIKIPLPPLEEQKAIAKRLKAIDDLIEIKRKE 401
Query: 405 KEKLERMKKAVMEKLLTGEVRVR 427
KE++E+ KK +M LLTG++RV+
Sbjct: 402 KEQIEKAKKKIMNLLLTGKIRVK 424
>sp|Q58615|YC18_METJA HYPOTHETICAL PROTEIN MJ1218 >gi|2129338|pir||A64452 type I
restriction-modification enzyme, S subunit homolog -
Methanococcus jannaschii >gi|1591847|gb|AAB99219.1|
(U67562) type I restriction-modification enzyme 1, S
subunit [Methanococcus jannaschii]
Length = 425
Score = 162 bits (407), Expect = 5e-39
Identities = 134/438 (30%), Positives = 209/438 (47%), Gaps = 41/438 (9%)
Query: 8 FYRETRFKEVELNGRKARIPEEWEVVELGEVARIRKKKSVRDIAEVAVIPMEKVPQDNEL 67
FY+E FKE+ R+PE+WEVV +G+ + K K + + + L
Sbjct: 7 FYKEENFKEMH----GLRVPEDWEVVRIGDFIKYIKGKKPAVMVDEELEGYYPYLSTEYL 62
Query: 68 FAEFEIKAIEDVKSSTYCEAGDLLL---AKITPSFENGKQGIVPFNVPNGFALATTEVYP 124
K ++ D+LL GK+GI L++T V
Sbjct: 63 RDGIASKFVKITNKEIIVNENDILLLWDGSNAGEIFLGKKGI----------LSSTMVKL 112
Query: 125 IVPSENLDVFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKIA 184
++ +D +LFY LK K L+ + GT G V K ++IPLPPLEEQK+IA
Sbjct: 113 EQKNKIMDDLYLFYSLKLKE--SFLKSQTKGT-GIPHVDKKIFENIKIPLPPLEEQKQIA 169
Query: 185 EILRSIDEAIQAVDESIARLERLKKGTMERLLTRGI-NHTRFKTVELNGRKVEIPEEWDV 243
+IL D I +++ I L + KKG M++L T+G+ H FK E+ EIPE+W+V
Sbjct: 170 KILSDFDNLIGTINKQIEVLNKAKKGMMKKLFTKGVFEHKSFKKSEIG----EIPEDWEV 225
Query: 244 VELGEVAERRNESVNPANMGNIPFVGLEHIEPGNIRLSQ--WGNSSEVKSSKSKFYP--- 298
V+L EV + +S G++ ++ N+ + W S + YP
Sbjct: 226 VKLKEVVDI--QSGKYFKYSEFCENGVKCLKIDNVGFGKIFWETVSFLPEDYLNKYPQLV 283
Query: 299 ---GDILYGKLRPYLD-KAVIADFEGICSTDIIVIKA-----KEDKTIPEYLIWVIHSKE 349
GDI+ RP + K I + I I+ + K +K ++L +++ S+
Sbjct: 284 LKSGDIVLALNRPIIGGKIKIGILKDIDEPAILYQRVGRFIFKSEKIDKQFLFYLLMSEY 343
Query: 350 FIEYAKKTMKGVNHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLE 409
F + K + G + P + +IPLP LEEQK +AE L++ID IE KR +KE++E
Sbjct: 344 FKKELSKLLIGTDQPYIRTPVLLNIKIPLPHLEEQKAMAERLKSIDNLIEIKRKEKEQIE 403
Query: 410 RMKKAVMEKLLTGEVRVR 427
+ KK +M LLTG++RV+
Sbjct: 404 KAKKKIMNLLLTGKIRVK 421
>pir||B69226 type I restriction modification system, subunit S -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2622039|gb|AAB85439.1| (AE000868) type I restriction
modification system, subunit S [Methanobacterium
thermoautotrophicum]
Length = 407
Score = 144 bits (359), Expect = 2e-33
Identities = 101/316 (31%), Positives = 164/316 (50%), Gaps = 40/316 (12%)
Query: 125 IVPSENLDV-FFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKI 183
+VP ++D +F +Y+L +K K L+ G+T ++ +KT L K+ +P+PPLEEQK+I
Sbjct: 116 LVPKSHVDTRYFAYYLLINK---KRLQDLSGGSTFKELNKKT-LEKIYLPVPPLEEQKRI 171
Query: 184 AEILRSIDEAIQAVDESIARLERLKKGTMERLLTRGINHTRFKTVELNGRKVEIPEEWDV 243
+EIL+ +D AI+ V++ I E+LK+G M+RLL GINHT FK + IP +WDV
Sbjct: 172 SEILQDVDGAIEKVNKEIGVTEKLKRGLMQRLLMEGINHTEFKDSHVG----RIPVDWDV 227
Query: 244 VELGEVAERRNESVNPAN-------MGNIPFVGLEHIEPGNIRLSQWGNSSEVKSSKSKF 296
V L +V E + P + G+ P+ G I +
Sbjct: 228 VNLEDVVEIHDNKRIPLSEKERIKMKGDYPYCGANGI---------------IDYINDYI 272
Query: 297 YPGDILY-----GKLRPYLDKAVIADFEGICSTDIIVIKAKEDKTIPEYLIWVIHSKEFI 351
+ G+ + G + A I + + + VI+A K +L+ ++ +
Sbjct: 273 FNGEFVLLAEDGGDYSSFGSSAYIMNGKFWVNNHAHVIEALPSKITNRFLLHILIYLDLT 332
Query: 352 EYAKKTMKGVNHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERM 411
Y + G + + +++ +IPLPPLEEQK+I+EIL+ +D +E +K KLE +
Sbjct: 333 HY----VVGSTRKKLNQGIMRKIKIPLPPLEEQKRISEILQDVDRRLELLTERKVKLENI 388
Query: 412 KKAVMEKLLTGEVRVR 427
K+ +M LLTG+ RVR
Sbjct: 389 KRGLMNDLLTGKRRVR 404
>pir||C82522 type I restriction-modification system specificity determinant
XF2741 [imported] - Xylella fastidiosa (strain 9a5c)
>gi|9107979|gb|AAF85526.1|AE004080_8 (AE004080) type I
restriction-modification system specificity determinant
[Xylella fastidiosa]
Length = 412
Score = 141 bits (351), Expect = 2e-32
Identities = 95/327 (29%), Positives = 164/327 (50%), Gaps = 29/327 (8%)
Query: 123 YPIVPSENLDVFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKK 182
Y +VP +LD+ +L+Y +K + ++ + +T R V L++ +PP EQ
Sbjct: 81 YYLVPKTDLDMRWLYYAVKHYKLGEVDDGSPIPSTTRAAVYM-----LELDVPPKHEQHA 135
Query: 183 IAEILRSIDEAIQAVDESIARLERLKKGT--------------MERLLTRGIN------- 221
IA+IL ++D+ I+ + LE + + +E RG +
Sbjct: 136 IAKILGTLDDKIELNRRTNETLEAMARALFKAWCVDFEPVRAKLEGRWQRGESLPGLPAH 195
Query: 222 -HTRFKTVELNGRKVEIPEEWDVVELGEVAERRNESVNPANMGN-IPFVGLEHIEPGNIR 279
+ F + EIPE W V LG+VA SV P+ + + ++ LEH+ I
Sbjct: 196 LYDLFPARLIESEWGEIPEGWRVDSLGKVAVHLRRSVQPSEIKDETSYIALEHMPKRCIA 255
Query: 280 LSQWGNSSEVKSSKSKFYPGDILYGKLRPYLDKAVIADFEGICSTDIIVIKAKEDKTIPE 339
L++WG ++ ++S+K +F G+IL+GKLRPY K +A +G+CSTDI+VI A T
Sbjct: 256 LAEWGVANGIESNKYEFKQGEILFGKLRPYFHKVGVAPVDGVCSTDIVVI-APILPTWFG 314
Query: 340 YLIWVIHSKEFIEYAKKTMKGVNHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIE 399
+++ + S F+EY G PRTSW + Q+ + LP + + ++ + E I
Sbjct: 315 FVLVHVSSDAFVEYTNAGSTGTKMPRTSWSEMAQYPVVLPHEDVAVAFNQHIQALSEEII 374
Query: 400 AKRAKKEKLERMKKAVMEKLLTGEVRV 426
K + L +++ ++ KL++GE+RV
Sbjct: 375 IKIHESRSLVQLRDTLLPKLISGELRV 401
>sp|Q57594|Y130_METJA HYPOTHETICAL PROTEIN MJ0130 >gi|2826248|gb|AAB98112.1| (U67470)
type I restriction-modification enzyme 2, S subunit
[Methanococcus jannaschii]
Length = 343
Score = 132 bits (330), Expect = 5e-30
Identities = 91/255 (35%), Positives = 144/255 (55%), Gaps = 29/255 (11%)
Query: 8 FYRETRFKEVELNGRKARIPEEWEVVELGEVARIRK-----KKSVRDIAEVAVIP---ME 59
FY+E FK+ E+ IPE+WE+VEL +V + K K SV + + IP +E
Sbjct: 2 FYKEENFKKTEIG----EIPEDWEIVELKDVCKKIKAGGTPKTSVEEYYKNGTIPFVKIE 57
Query: 60 KVPQDNELFAEFEIKAIEDVKSSTYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALAT 119
+ N+ +IK E+ +++ ++ K + F G + N +AT
Sbjct: 58 DITNSNKYLTNTKIKITEEGLNNSNA----WIVPKNSVLF--AMYGSIGETAINKIEVAT 111
Query: 120 TE-VYPIVPSEN-LDVFFLFYIL-KDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPP 176
+ + I+P +N L+ FL+YIL K+K + L ++ T ++ K+ +IPLPP
Sbjct: 112 NQAILGIIPKDNILESEFLYYILAKNKNYYSKLGMQTTQKNLNAQIVKS----FKIPLPP 167
Query: 177 LEEQKKIAEILRSIDEAIQAVDESIARLERLKKGTMERLLTRGINHTRFKTVELNGRKVE 236
LEEQK+IA+IL IDE I+ +++SI +LER+KKG M +LLT+GI H+RFK E+ E
Sbjct: 168 LEEQKQIAKILTKIDEGIEIIEKSINKLERIKKGLMHKLLTKGIGHSRFKKSEIG----E 223
Query: 237 IPEEWDVVELGEVAE 251
IPE+W+V E+ ++ E
Sbjct: 224 IPEDWEVFEIKDIFE 238
Score = 89.7 bits (219), Expect = 6e-17
Identities = 66/211 (31%), Positives = 103/211 (48%), Gaps = 24/211 (11%)
Query: 225 FKTVELNGRKVEIPEEWDVVELGEVAERRNESVNPANM-------GNIPFVGLEHIEPGN 277
FK E+ EIPE+W++VEL +V ++ P G IPFV +E I N
Sbjct: 8 FKKTEIG----EIPEDWEIVELKDVCKKIKAGGTPKTSVEEYYKNGTIPFVKIEDITNSN 63
Query: 278 -------IRLSQWGNSSEVKSSKSKFYPGDILYGKLRPYLDKAVIADFEGICSTDIIVIK 330
I++++ G + +S + P + + + + + I E + I+ I
Sbjct: 64 KYLTNTKIKITEEG----LNNSNAWIVPKNSVLFAMYGSIGETAINKIEVATNQAILGII 119
Query: 331 AKEDKTIPEYLIWVIHSKEFIEYAKKTMKGVNHPRTSWKSIKQFQIPLPPLEEQKKIAEI 390
K++ E+L +++ + Y K + + +K F+IPLPPLEEQK+IA+I
Sbjct: 120 PKDNILESEFLYYILAKNK--NYYSKLGMQTTQKNLNAQIVKSFKIPLPPLEEQKQIAKI 177
Query: 391 LRTIDEAIEAKRAKKEKLERMKKAVMEKLLT 421
L IDE IE KLER+KK +M KLLT
Sbjct: 178 LTKIDEGIEIIEKSINKLERIKKGLMHKLLT 208
>gb|AAG18734.1| (AE004978) type I restriction modification enzyme, S subunit; RmeS
[Halobacterium sp. NRC-1]
Length = 475
Score = 132 bits (330), Expect = 5e-30
Identities = 116/422 (27%), Positives = 194/422 (45%), Gaps = 28/422 (6%)
Query: 27 PEEWEVVELGEVARI--RKKKSVRDIAEVAVIPMEKVPQDNELFAEF---EIKAIEDVKS 81
P EW LG++ ++ R K+ D V VI E + D F E E K
Sbjct: 43 PGEWTAKRLGDIKQLITRGKQPTYDDDGVPVINQECIYWDGWHFENLRYLEEDVAEGWKE 102
Query: 82 STYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATTEVYPIVPSENLDVFFLFYILK 141
+ E+GD+++ T G+ + P + + V + E L F Y +
Sbjct: 103 KYFPESGDVIVNS-TGQGTLGRAQVYPGDQRRAI---DSHVTLLRTDEQLCPHFHRYFFE 158
Query: 142 DKRFRKIL-EVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKIAEILRSIDEAIQAVDES 200
+ +L + + G+TG+ + KT L L PLPPLEEQ+KIA +L ++D+AIQ +
Sbjct: 159 SHLGQALLYSMCVNGSTGQIELSKTRLDLLPTPLPPLEEQRKIASVLYNVDQAIQKTEAV 218
Query: 201 IARLERLKKGTMERLLTRGI---NHTRFKTVELNG-----RKVEIPEEWDVVELGEVAER 252
I ++ERLK+G ++ L T+G+ N R + G R+ IP EW+V L +
Sbjct: 219 IEKIERLKQGLLDDLFTKGLSESNSLRPSPEDHPGLYKKERRQTIPSEWNVESLQNLCVE 278
Query: 253 RNE----SVNPANMGNIPFVGLEHIEPGNI---RLSQWGNSSEVKSSKSKFYPGDILYGK 305
P +P++ E + G+I LS+ K S+S+ + +++
Sbjct: 279 NITYGIVQPGPHVEDGVPYINTEDMTDGDIPTEGLSRTSPEIAEKYSRSQIHAEELVV-T 337
Query: 306 LRPYLDKA--VIADFEGICSTDIIVIKAKEDKTIPEYLIWVIHSKEFIEYAKKTMKGVNH 363
+R + V + EG T DK +L+W I S F +KG
Sbjct: 338 IRATIGAVDQVPPELEGANLTRGTARVVPGDKVDNTFLLWAIRSNNFQSELDARVKGTTF 397
Query: 364 PRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERMKKAVMEKLLTGE 423
+ + + +P P ++EQ +I + L TI+E +E + + E+L+R+K+ +M+ LL+GE
Sbjct: 398 DEINLDQLGKIPVPHPDIDEQDRIVDELSTIEERMENEESYLEQLKRLKQGLMQDLLSGE 457
Query: 424 VR 425
VR
Sbjct: 458 VR 459
>sp|P06187|T1S_SALTY TYPE I RESTRICTION ENZYME STYSJI SPECIFICITY PROTEIN (S PROTEIN)
(S.STYSJI) >gi|47739|emb|CAA68580.1| (Y00524) S
polypeptide [Salmonella typhimurium]
Length = 469
Score = 116 bits (287), Expect = 6e-25
Identities = 107/431 (24%), Positives = 189/431 (43%), Gaps = 39/431 (9%)
Query: 25 RIPEEWEVVELGEVARIRKKKSVRDIAEVAVIPMEKVPQDNELFAEFEIKAIEDVKSS-T 83
++PE W + E+ + K + D +V +PM VP FE K +VK T
Sbjct: 5 KLPEGWATSTINEMCNLNPKLKLDDDLDVGFMPMAGVPTTYLGKCNFETKKWSEVKKGFT 64
Query: 84 YCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATTEVYPIVPSENL-DVFFLFYILKD 142
+ D++ AKITP FENGK ++ PNG+ +TE Y + L + +LF ++K
Sbjct: 65 QFQNDDVIFAKITPCFENGKAVVIK-EFPNGYGAGSTEYYVLRSINGLINPHWLFALVKT 123
Query: 143 KRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKIAEILRSIDEAIQAVDESIA 202
K F + M+G+ G +RV K L +P+PPL EQK IAE +D + VD + A
Sbjct: 124 KDFLTNGALNMSGSVGHKRVTKEFLENYGVPVPPLAEQKVIAE---KLDTLLAQVDSTKA 180
Query: 203 RLERLK---KGTMERLLTRGIN---------HTRFKTVELNGRKVEIPEEWDVVELGEVA 250
RLE++ K + ++ +N +FK ELN + IP W + E+G+ A
Sbjct: 181 RLEQIPQILKRFRQSVIVAAVNGQLTKELHKKNKFKLTELN---ISIPSLWKISEIGQFA 237
Query: 251 E-------RRNESVNPANMGNIPFVGLEHIEPGNI-RLSQWGNSSEVKSSKSKF--YPGD 300
+ + ES+ N G P++ ++ G + Q ++ S S++ GD
Sbjct: 238 DVKGGKRLPKGESLIAENTG-FPYIRAGQLKNGTVLPEGQLYLEEYIQKSISRYTVSSGD 296
Query: 301 ILYGKLRPYL-DKAVIADF--EGICSTDIIVIKAKEDKTIPEYLIWVIHSKEFIEYAKKT 357
+ + + D +I D + + I + +L + S +
Sbjct: 297 LYITIVGACIGDAGIIPDVYNNANLTENAAKICNLNENIFNRFLSLWLRSSYLQDIINSE 356
Query: 358 MKGVNHPRTSWKSIKQFQIPLPPLEEQ----KKIAEILRTIDEAIEAKRAKKEKLERMKK 413
+K + + IK + LPPL+EQ +++ ++ D + ++ + +
Sbjct: 357 IKSGAQGKLALARIKSLPLILPPLQEQHEIVRRVEQLFAYADTIEKQVNNALTRVNSLTQ 416
Query: 414 AVMEKLLTGEV 424
+++ K GE+
Sbjct: 417 SILAKAFRGEL 427
>gi|10956235 HsdS [Lactococcus lactis subsp. lactis] >gi|9294803|gb|AAF86681.1|
(AF179848) HsdS [Lactococcus lactis subsp. lactis]
Length = 402
Score = 108 bits (268), Expect = 1e-22
Identities = 81/268 (30%), Positives = 132/268 (49%), Gaps = 16/268 (5%)
Query: 155 GTTGRQRVQKTDLLKLQ-IPLPPLEEQKKIAEILRSIDEAIQAVDESIARLERLKKGTME 213
G G Q ++L++ Q I LP +EQ+KI + +D I + L+ KKG ++
Sbjct: 146 GQPGSQVNLNSELVRNQEIVLPNYKEQQKIGSFFKQLDNTITLHQRKLDLLKEQKKGYLQ 205
Query: 214 RLLTRGINHTRFKTVELNGRKVEIPEEWDVVELGEVAERRNESVNPANMGNIPFVGLEHI 273
++ + K EL R ++W+ +L ++ R ++S +N +P V E I
Sbjct: 206 KMFPKN----GAKVPEL--RFAGFADDWEERKLSDIVSRLSKS---SNNSELPRVEFEDI 256
Query: 274 EPGNIRLSQWGNSSEVKSSKSKFYPGDILYGKLRPYLDKAVIADFEGICSTDIIVIKAKE 333
G RL++ + F +ILYGKLRPYL ++ADF+GI D V K+
Sbjct: 257 VSGEGRLNKDVSHKFDDRKGILFSSQNILYGKLRPYLKNWLLADFKGIALGDFWVFKSIN 316
Query: 334 DKTIPEYLIWVIHSKEFIEYAKKTMKGVNHPRTSWKSIK--QFQIPLPPLEEQKKIAEIL 391
P+++ +I S + + A T G PR+ WK + +FQIP LEEQKKI
Sbjct: 317 SD--PKFVYSLIQSNHYQKVANDT-SGTKMPRSDWKKVSSTEFQIP-SSLEEQKKIGSFF 372
Query: 392 RTIDEAIEAKRAKKEKLERMKKAVMEKL 419
+ +D+ I + K + L+ KK ++K+
Sbjct: 373 KKLDDTIALHQRKLDLLKEQKKGFLQKM 400
>sp|P06991|T1SD_ECOLI TYPE I RESTRICTION ENZYME ECODI SPECIFICITY PROTEIN (S PROTEIN)
(S.ECODI) >gi|41744|emb|CAA23553.1| (V00287) hsdS
[Escherichia coli]
Length = 444
Score = 103 bits (255), Expect = 3e-21
Identities = 95/425 (22%), Positives = 181/425 (42%), Gaps = 52/425 (12%)
Query: 25 RIPEEWEVVELGEVARIRKKKSVRDIAEVAVIPMEKVPQDNELFAEFEIKAIEDVKSSTY 84
++P +W+ VELGE+ ++ K + A+ D + +++ + S +
Sbjct: 5 KLPVDWKTVELGELIKLSTGKLDANAAD----------NDGQYPFFTCAESVSQINSWAF 54
Query: 85 CEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATTEVYPIVPSENLDVFFLFYILKDKR 144
+ LL NG I + T + PI+ + FL+++L+
Sbjct: 55 DTSAVLLAG-------NGSFSIKKYTGKFNAYQRTYVIEPIL----IKTEFLYWLLRGN- 102
Query: 145 FRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKIAEILRSIDEAIQAVDESIARL 204
+KI E T ++K D+ + + LP EQ IAE L ++ +++ + ++
Sbjct: 103 IKKITENGRGSTI--PYIRKGDITDISVALPSPSEQTLIAEKLDTLLAQVESTKARLEQI 160
Query: 205 ERLKKGTMERLLTRGIN------------HTRFKTVELNGRKV-------EIPEEWDVVE 245
++ K + +LT +N + F E N K IP W +
Sbjct: 161 PQILKRFRQAVLTFAMNGELTKEWRSQNNNPAFFPAEKNSLKQFRNKELPSIPNNWSWMR 220
Query: 246 LGEVAERRNESVNPANMGNIPFVGLEHIEPGNIRLSQWGNSSE--VKSSKSKFYPGDILY 303
+VA+ ++ +P + N + HIE + S + E V S+K +FY G I+Y
Sbjct: 221 FDQVADIASKLKSPLDYPNTIHLAPNHIESWTGKASGYQTILEDGVTSAKHEFYTGQIIY 280
Query: 304 GKLRPYLDKAVIADFEGICSTDIIVIKAKEDKTIPEYLIWVIHSKEFIEYAKKTMKGVNH 363
K+RPYL K IA F+G+CS D+ I +K D +L + + F ++A
Sbjct: 281 SKIRPYLCKVTIATFDGMCSADMYPINSKID---THFLFRWMLTNTFTDWASNAESRTVL 337
Query: 364 PRTSWKSIKQFQIPLPPLEEQ----KKIAEILRTIDEAIEAKRAKKEKLERMKKAVMEKL 419
P+ + K + + +P PPL EQ +++ ++ D + ++ + ++++ K
Sbjct: 338 PKINQKDLSEIPVPTPPLPEQHEIVRRVEQLFAYADTIEKQVNNALARVNNLTQSILAKA 397
Query: 420 LTGEV 424
GE+
Sbjct: 398 FRGEL 402
>pir||E83304 hypothetical protein PA2734 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9948811|gb|AAG06122.1|AE004701_5
(AE004701) hypothetical protein [Pseudomonas aeruginosa]
Length = 431
Score = 100 bits (246), Expect = 4e-20
Identities = 70/281 (24%), Positives = 129/281 (44%), Gaps = 17/281 (6%)
Query: 159 RQRVQKTDLLKLQIPLPPLEEQKKIAEILRSIDEAI----QAVDESIARLERLKKGTMER 214
R R+ ++ +PP EQ +I LR+ D I +A + IA L K ++
Sbjct: 121 RNRLYWESFKQMPSLVPPRPEQDQIVTYLRTQDAHIACFIRAKRDLIALLTEQKLRIIDH 180
Query: 215 LLTRGINHTRFKTVELNGRKVE----IPEEWDVVELGEVAERRNESVNPANMGNIPFVGL 270
+TRG++ +V+L +E +P W+V L +A I ++ L
Sbjct: 181 AVTRGLD----ASVKLKPSDIEWLGEVPAHWEVKRLKFLAGNITSQTTTKADDEI-YLAL 235
Query: 271 EHIEPGNIRLSQWGNSSEVKSSKSKFYPGDILYGKLRPYLDKAVIADFEGICSTDIIVIK 330
EH++ G E S+ +F D+L+GKLRPYL K G+C ++ +V++
Sbjct: 236 EHVQSWTGVARPLGGEVEFASTVKRFVADDVLFGKLRPYLAKVTRVVCAGVCVSEFLVLR 295
Query: 331 AKEDKTIPEYLIWVIHSKEFIEYAKKTMKGVNHPRTSWKSIKQFQIPLPPLEEQKKIAEI 390
++++ +P YL ++ K I+ + G PR W I ++P+P +EQ+ I
Sbjct: 296 SRQELILPAYLEQLLRCKRVIDLISSSTAGAKMPRADWNFIGNVRLPIPRKDEQEAILSH 355
Query: 391 L----RTIDEAIEAKRAKKEKLERMKKAVMEKLLTGEVRVR 427
+ + +DE I + + + + ++ +TG+V +R
Sbjct: 356 IGRETKDLDETIARAEDEIKLIREYRDRLIADAVTGQVDLR 396
>pir||NDECAS type I site-specific deoxyribonuclease (EC 3.1.21.3) EcoA chain S -
Escherichia coli
Length = 589
Score = 91.7 bits (224), Expect = 1e-17
Identities = 61/171 (35%), Positives = 90/171 (51%), Gaps = 4/171 (2%)
Query: 16 EVELNGRKARIPEEWEVVELGEVARIRKKKSVRDIA-EVAVIPMEKVPQDNELFAEFEIK 74
E+ + +P+ WE L +A I K V D E++ IPM + + EFEIK
Sbjct: 91 EISEEEKPFELPDGWEWTTLTRIAEINPKIDVSDDEQEISFIPMPLISTKFDGSHEFEIK 150
Query: 75 AIEDVKSS-TYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATTEVYPIVP-SENLD 132
+DVK T+ GD+ +AKITP FEN K I + NG + TTE++ P S+ ++
Sbjct: 151 KWKDVKKGYTHFANGDIAIAKITPCFENSKAAIFS-GLKNGIGVGTTELHVARPFSDIIN 209
Query: 133 VFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKI 183
+L K F K E +MTG+ G++RV + IP PPL+EQ++I
Sbjct: 210 RKYLLLNFKSPNFLKSGESQMTGSAGQKRVPRFFFENNPIPFPPLQEQERI 260
>sp|P19704|T1SA_ECOLI TYPE I RESTRICTION ENZYME ECOAI SPECIFICITY PROTEIN (S PROTEIN)
(S.ECOAI) >gi|146402|gb|AAA23987.1| (J03150) EcoA type I
restriction-modification enzyme S subunit [Escherichia
coli]
Length = 589
Score = 91.7 bits (224), Expect = 1e-17
Identities = 61/171 (35%), Positives = 90/171 (51%), Gaps = 4/171 (2%)
Query: 16 EVELNGRKARIPEEWEVVELGEVARIRKKKSVRDIA-EVAVIPMEKVPQDNELFAEFEIK 74
E+ + +P+ WE L +A I K V D E++ IPM + + EFEIK
Sbjct: 91 EISEEEKPFELPDGWEWTTLTRIAEINPKIDVSDDEQEISFIPMPLISTKFDGSHEFEIK 150
Query: 75 AIEDVKSS-TYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATTEVYPIVP-SENLD 132
+DVK T+ GD+ +AKITP FEN K I + NG + TTE++ P S+ ++
Sbjct: 151 KWKDVKKGYTHFANGDIAIAKITPCFENSKAAIFS-GLKNGIGVGTTELHVARPFSDIIN 209
Query: 133 VFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKI 183
+L K F K E +MTG+ G++RV + IP PPL+EQ++I
Sbjct: 210 RKYLLLNFKSPNFLKSGESQMTGSAGQKRVPRFFFENNPIPFPPLQEQERI 260
>pir||H81307 restriction modification enzyme Cj1051c [imported] - Campylobacter
jejuni (strain NCTC 11168) >gi|6968487|emb|CAB73307.1|
(AL139077) restriction modification enzyme [Campylobacter
jejuni]
Length = 1339
Score = 87.8 bits (214), Expect = 2e-16
Identities = 57/176 (32%), Positives = 89/176 (50%), Gaps = 4/176 (2%)
Query: 44 KKSVRDIAE---VAVIPMEKVPQDNELFAEFEIKAIEDVKSSTYCEAGDLLLAKITPSFE 100
KK + + E V+ I M V + ++ + E K TY D+L+AKITP E
Sbjct: 1163 KKEISKLDENMLVSFIEMASVSDKGYIQSKIDRSLNEVRKGYTYFIENDILIAKITPCME 1222
Query: 101 NGKQGIVPFNVPNGFALATTEVYPIVPSENLDVFFLFYILKDKRFRKILEVRMTGTTGRQ 160
NGK I N+ N +TE + LD FLFY L + R+ + MTG +G +
Sbjct: 1223 NGKCAIAK-NLTNNIGFGSTEFHIFRAKTGLDSSFLFYNLNQQNIREKAALAMTGASGHK 1281
Query: 161 RVQKTDLLKLQIPLPPLEEQKKIAEILRSIDEAIQAVDESIARLERLKKGTMERLL 216
RV + L IPLPPLE Q+KI + + +++ I ++ + LE+ K+ +++ L
Sbjct: 1282 RVPISFYENLTIPLPPLEIQEKIVQNIELVEQQIDLLNLKLEFLEKEKEKILQKYL 1337
>gb|AAG22014.1|AF288037_3 (AF288037) putative HsdS [Streptococcus thermophilus]
Length = 402
Score = 84.7 bits (206), Expect = 2e-15
Identities = 79/311 (25%), Positives = 136/311 (43%), Gaps = 33/311 (10%)
Query: 135 FLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIP-LPPLEEQKKIAEILRSIDEA 193
FL+Y L+ F + G+T + R+ +L +IP +P +++Q +I +IL ++D+
Sbjct: 105 FLYYFLQTNPF-----ITTNGSTIK-RLYNDNLRDTKIPNVPSIQQQNQITDILGTLDKK 158
Query: 194 IQAVDESIARLERLKKGTMERLLTRGINHTRFKTVELNGR---------------KVEIP 238
IQ ++ LE + K + + F + NG+ K EIP
Sbjct: 159 IQINNQINQELEAMAKTLYDYWFVQ------FDFPDQNGKPYKSSGGKMVYNPELKREIP 212
Query: 239 EEWDVVELGEVAERRNESVNPANMGNIPFVGLEHIEPGNIRLSQWGNSSEVKSSKSKFYP 298
E W +L + + E+ NP N F E +KS+K K
Sbjct: 213 EGWGAEKLSSLLKIGKETTNPKKFPNEEFKYYSIPEFDTTGTYSLERGESIKSNKFKVEK 272
Query: 299 GDILYGKLRPYLDKAVI-ADFEGICSTDIIVIKAKEDKTIPEYLIWVIHSKEFIEYAKKT 357
D+L KL P+ ++ + + I ST+ IV K ++ +L V KEFIEY +
Sbjct: 273 NDLLVSKLNPWFNRVIYNLEENAIASTEFIVWKTF-NRFEKNFLYQVATGKEFIEYCTRF 331
Query: 358 MKGV--NHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERMKKAV 415
G +H R S + FQIP Q K EI+ +I + + ++L +++ +
Sbjct: 332 ATGTSNSHKRVSPDIMVGFQIPFEKTHIQ-KFGEIIDSIRTQVLQNNEQNQELTQLRDWI 390
Query: 416 MEKLLTGEVRV 426
+ L+ G+V+V
Sbjct: 391 LPMLMNGQVKV 401
>gi|10954528 M. jannaschii predicted coding region MJECL41 [Methanococcus
jannaschii] >gi|2129054|pir||H64514 hypothetical protein
MJECL41 - Methanococcus jannaschii plasmid pURB800
>gi|1522674|gb|AAC37110.1| (L77118) M. jannaschii
predicted coding region MJECL41 [Methanococcus
jannaschii]
Length = 432
Score = 81.5 bits (198), Expect = 2e-14
Identities = 113/458 (24%), Positives = 195/458 (41%), Gaps = 74/458 (16%)
Query: 8 FYRETRFKEVELNGRKARIPEEWEVVELGEVARIRKKKSVRDIAEVA--VIPMEKV---- 61
F ET FKE ++ +IP++W+V K ++DI EVA P K+
Sbjct: 7 FRWETEFKETDIG----KIPKDWDV------------KKIKDIGEVAGGSTPSTKIKEYW 50
Query: 62 --------PQDNELFAEFEI---------KAIEDVKSSTYCEAGDLLLAKITPSFENGKQ 104
P+D + I KA+++ + + G +LL P G
Sbjct: 51 GGDIPWITPKDLANYEYIYISRGERNITEKAVKECSLRIFPK-GTILLTSRAPI---GYV 106
Query: 105 GIV--PFNVPNGFALATTEVYPIVPSENLDVFFLFYILKDKRFRKILEVRMTGTTGRQRV 162
I P GF I+P + + +L+Y+ K K + L+ G+T + +
Sbjct: 107 AIAKNPLTTNQGFR-------NIIPKDGVVSEYLYYLFKTKTMSEYLKDISGGSTFPE-L 158
Query: 163 QKTDLLKLQIPLPPLEEQKKIAEILRSIDEAIQAVDESIARLERLKKGTMERLLTRGINH 222
+ + L +++IP P EEQ+KIA +L D+ I+ + LE++ +E I+
Sbjct: 159 KGSTLKEVEIPYPSPEEQQKIATVLSYFDDLIENKKKQNEILEKI---ALELFKNWFIDF 215
Query: 223 TRFKTVEL---NGRKVEIPEEWDVVELGEVAERRNESVNPANM-----GNIPFVGLEHIE 274
FK E + EIP+ W+V LG++ + + S P IPFV ++H+
Sbjct: 216 EPFKNEEFVYNDELDKEIPKGWEVKRLGDILKVESGSNAPQREIYFENAKIPFVRVKHLV 275
Query: 275 PGN-IRLSQWGNSSEVKSSKSKFY-PGDILYGKLRPYLDKAV--IADFEGICSTDIIVIK 330
G I S + N +K K K Y I++ K L +A I F+ + VI
Sbjct: 276 KGVCIESSDFINELALKDYKMKLYNEKSIIFQKSGESLKEARVNIVPFKFTAVNHLAVID 335
Query: 331 AK--EDKTIPEYLIWVIHSKEFIEYAKKTMKGVNHPRTSWKSIKQFQIPLPPLEEQKKIA 388
+ +K Y + KE + ++KG P I+ I +PP +K
Sbjct: 336 SSMLNEKHYFIYCLLRFLLKEIVY----SVKGTTLPYLKISDIENKYIIIPPQPILQKFH 391
Query: 389 EILRTIDEAIEAKRAKKEKLERMKKAVMEKLLTGEVRV 426
+++ + E I + + L++++ A++ KL+ GE+RV
Sbjct: 392 SLVQPLFEKIINNQKQIMVLKKIRDALLPKLVFGELRV 429
>sp|P19705|T1SE_ECOLI TYPE I RESTRICTION ENZYME ECOEI SPECIFICITY PROTEIN (S PROTEIN)
(S.ECOEI) >gi|67291|pir||NDECES type I site-specific
deoxyribonuclease (EC 3.1.21.3) EcoE chain S -
Escherichia coli >gi|146400|gb|AAA23986.1| (J03162) EcoE
type I restriction modification enzyme S subunit
[Escherichia coli]
Length = 594
Score = 81.5 bits (198), Expect = 2e-14
Identities = 54/161 (33%), Positives = 85/161 (52%), Gaps = 4/161 (2%)
Query: 26 IPEEWEVVELGEVARIRKKKSVRDIA-EVAVIPMEKVPQDNELFAEFEIKAIEDVKSS-T 83
+PE WE + L E+A I K V D E++ +PM + + + EIK +VK T
Sbjct: 101 LPEGWEWITLSEIATINPKIEVTDDEQEISFVPMPCISTRFDGAHDQEIKKWGEVKKGYT 160
Query: 84 YCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATTEVYPIVP-SENLDVFFLFYILKD 142
+ GD+ LAKITP FEN K ++ + G + TTE++ P S L++ ++ +K
Sbjct: 161 HFADGDIALAKITPCFENSK-AVIFKGLKGGVGVGTTELHVARPISSELNLQYILLNIKS 219
Query: 143 KRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKI 183
+ + E MTG+ G++RV ++ IP PP EQ +I
Sbjct: 220 PHYLSMGESMMTGSAGQKRVPRSFFENYPIPFPPNTEQARI 260
>pir||H82160 conserved hypothetical protein VC1768 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9656290|gb|AAF94917.1|
(AE004253) conserved hypothetical protein [Vibrio
cholerae]
Length = 462
Score = 80.8 bits (196), Expect = 3e-14
Identities = 76/325 (23%), Positives = 147/325 (44%), Gaps = 18/325 (5%)
Query: 116 ALATTEVYPIVPSENLDVFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLP 175
A+ + V + P E + FL Y+ + + + TTG Q + + L + +P
Sbjct: 138 AVTSNFVAKMTPKEWVISGFLKYVFSALYNNGVNYLSIKQTTGIQNLDASSYLNEKFCIP 197
Query: 176 PLEEQKKIAEILRS----IDEAIQAVDESIARLERLKKGTMERLLTRGINHT---RFKTV 228
EEQ +IA+ L + I+EAI + I L+ K+ +++ +T+G+N ++ V
Sbjct: 198 QKEEQYEIAKFLDNKTTQINEAIAIKQKQIELLKERKQIIIQQAVTQGLNPDATMKYSGV 257
Query: 229 ELNGRKVEIPEEWDVVELGEVAERRNESVNPANMGNIPFVGLEHIEPGNIRLSQWGNSSE 288
+ G IP W V + + NE + + + P + + + +
Sbjct: 258 DWIGA---IPGHWIVKRAKYLLDEINERSETGLEELLSVSHMTGVTPRSEKNVTMFMAED 314
Query: 289 VKSSKSKFYPGDILYGKLRPYLDKAVIADFEGICSTDIIVIKAKEDKT-IPEYLIWVIHS 347
SK + GD++ + ++ ++D GI S V + + + T +P+YL ++ S
Sbjct: 315 YTGSKL-CHSGDLVINIMWAWMGALGVSDRTGIVSPSYGVFREQREGTFVPKYLEMLLKS 373
Query: 348 KEFIEYAKKTMKGVNHPRTSWKSIKQFQIPL--PPLEEQKKIAEIL----RTIDEAIEAK 401
+++EY K G++ R + F + L PP EEQ +I E + +DEAI +
Sbjct: 374 TKYVEYYNKVSTGLHSSRLRFYGHMLFDMALGFPPYEEQTQIVEYISRECSKVDEAITVQ 433
Query: 402 RAKKEKLERMKKAVMEKLLTGEVRV 426
+ KL+ K ++ +TG+++V
Sbjct: 434 AEQVSKLKEYKTTLINSAVTGKIKV 458
>gb|AAF13313.1|AF109691_6 (AF109691) type I S-subunit [Lactobacillus delbrueckii subsp.
lactis]
Length = 389
Score = 78.4 bits (190), Expect = 1e-13
Identities = 81/393 (20%), Positives = 169/393 (42%), Gaps = 43/393 (10%)
Query: 43 KKKSVRDIAEVAVIPMEKVPQDNELFAEFEIKAIE-----DVKSSTYCEAGDLLLAK--- 94
+++ + D+ E ++ NE+FAE+ + A + D+ + + ++ +
Sbjct: 22 EQRKLGDVCEPITDSIDTQKYPNEVFAEYSMPAFDASMKPDIVLGSSMNSVRKIITRPCL 81
Query: 95 ITPSFENGKQGIVPFNVPNGFALATTEVYPIVPSENLDVFFLFYILKDKRFRKILEVRMT 154
+ K+ I PN A+ + E P+ S+ +D+ FL + K + F + LE +
Sbjct: 82 LVNKLNVRKKRIWYVKKPNKNAVCSAEFIPLY-SDTVDLTFLNQVAKSETFTRYLENHSS 140
Query: 155 GTTGRQ-RVQKTDLLKLQIPLPPLEEQKKIAEILRSIDEAIQAVDESIARLERLKKGTME 213
G++ Q R+ L+ ++ +P +EEQK I +I S+D I +E +LE LK ++
Sbjct: 141 GSSNSQKRITPRSLMLSKLHIPTIEEQKLIGKIFESLDHTITLHEEKKRQLECLKSALLQ 200
Query: 214 RLLTRGINHTRFKTVELNGRKVEIPEEWDVVELGEVAERRNESVNPANMGNIPFVGLEHI 273
++ + + + V G + W+ +L +V E++ + LE +
Sbjct: 201 KMFA---DKSGYPVVRFEG----FDKAWEERKLKDVVEKQIKGK----------AQLEKL 243
Query: 274 EPGNIRLSQWGNSSEVKSSKSKFYPG--DILYGKLRPYLDKA----VIADFEGICSTDII 327
PG + ++ ++S + ++ G D+ + D + V FEG + +
Sbjct: 244 APGEV---EYLDTSRLNGGQAILTNGLKDVTLDDILILWDGSKAGTVYHGFEGALGSTLK 300
Query: 328 VIK-AKEDKTIPEYLIWVIHSKEFIEYAKKTMKGVNHPRTSWKSIKQFQIPLPPLEEQKK 386
+ + K + +YL K + + N P + F I +P +EQ+K
Sbjct: 301 AYRTSANSKFVYQYL------KRHQDNIYNNYRTPNIPHVQKDFLNVFTISVPVSDEQEK 354
Query: 387 IAEILRTIDEAIEAKRAKKEKLERMKKAVMEKL 419
I + +D+ I + K + L+ KK ++K+
Sbjct: 355 IGSFFKQLDDTIAFHQRKLDLLKEQKKGFLQKM 387
>gb|AAC64909.1| (AF027167) putative type I S-subunit protein [Streptococcus
thermophilus]
Length = 412
Score = 74.9 bits (181), Expect = 2e-12
Identities = 71/304 (23%), Positives = 135/304 (44%), Gaps = 21/304 (6%)
Query: 125 IVPSEN-LDVFFLFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPPLEEQKKI 183
IVP +N LD +F+F + ++ EV G+T + V + K+ I +P L EQ+KI
Sbjct: 119 IVPDQNKLDSYFIFS--RTNELKRYGEVTGAGSTFVE-VSGKQMSKMSIMVPELSEQQKI 175
Query: 184 AEILRSIDEAIQAVDESIARLERLKKGTMERLLTRGINHTRFKTVELNGRKVEIPEEWDV 243
+ +DE I + L+ KKG ++++ + N + + L G ++W+
Sbjct: 176 GSFFKQLDETITLHQRKLDLLKEQKKGFLQKMFPK--NGAKVPELRLKG----FTDDWEE 229
Query: 244 VELGEVAERRNESVNPANMGNIPFVGLEHIEPGNIRLSQWGNSSE-------VKSSKSKF 296
+LGE+A + ++ P I + + S+ +K+S ++
Sbjct: 230 RKLGELANLVGGGTPRTSNPEYWDGDIDWYAPAEIGEQSYVSKSKKTITELGLKNSSARI 289
Query: 297 YP-GDILYGKLRPYLDKAVIADFEGICSTDIIVIKAKEDKTIPEYLIWVIHSKEFIEYAK 355
P G +L+ + A++A E + + I ++K + Y I+ + E Y +
Sbjct: 290 LPVGTVLFTSRAGIGNTAILAK-EATTNQGYLSIVPDQNK-LDSYFIF-SRTNELKRYGE 346
Query: 356 KTMKGVNHPRTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERMKKAV 415
T G S K + + I +P L EQ+KI + +D+ I + K + L+ KK
Sbjct: 347 VTGAGSTFVEVSGKQMSKMSIMVPELSEQQKIGLFFKHLDDTITFHQRKLDLLKEQKKGF 406
Query: 416 MEKL 419
++K+
Sbjct: 407 LQKM 410
>pir||JC5218 type I site-specific deoxyribonuclease (EC 3.1.21.3) Hsd chain S
[validated] - Pasteurella haemolytica
>gi|1685099|gb|AAC44667.1| (U46781) HSDS [Pasteurella
haemolytica]
Length = 442
Score = 73.0 bits (176), Expect = 6e-12
Identities = 46/173 (26%), Positives = 88/173 (50%), Gaps = 6/173 (3%)
Query: 248 EVAERRNESVNPANMGNIPFVGLEHIEP--GNIRLSQWGNSSEVKSSKSKFYPGDILYGK 305
++ E +E + ++ ++ +++ P G I L++ +S +S ++F DIL+
Sbjct: 10 DIVELISEKIKIKDLKKENYISTDNMLPNFGGITLAENLPNS---ASCNRFAKKDILFSN 66
Query: 306 LRPYLDKAVIADFEGICSTDIIVIKAKE-DKTIPEYLIWVIHSKEFIEYAKKTMKGVNHP 364
+R Y K +A+F G CS D++V+++K D + EYL +I S +FI + + G P
Sbjct: 67 IRTYFKKVWLAEFSGGCSPDVLVMRSKNTDILLNEYLFLLIRSDDFINFTVISANGAKMP 126
Query: 365 RTSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERMKKAVME 417
R ++K F +P +E QKK D+ I+ + LE + +A+ +
Sbjct: 127 RGDKNAMKGFIFNIPSIEYQKKCIANYFAFDQKIQLNTQTNQTLEAIAQAIFK 179
>gb|AAF69139.1|AF228680_3 (AF228680) HsdS [Lactococcus lactis]
>gi|9789464|gb|AAF98316.1|AF243383_17 (AF243383) HsdS
[Lactococcus lactis subsp. lactis]
Length = 421
Score = 69.9 bits (168), Expect = 5e-11
Identities = 78/354 (22%), Positives = 148/354 (41%), Gaps = 23/354 (6%)
Query: 76 IEDVKSSTYCEAGDLLLAKITPSFENGKQGIVPFNVPNGFALATTEVYPIVPSENLDVFF 135
+E KSS E GDL+ A GK V + + + F
Sbjct: 79 LEKYKSSLL-ENGDLIFADAAEDETVGKAVEVNGLTEENLVAGLHTIVARSKDKKAEFFL 137
Query: 136 LFYILKDKRFRKILEVRMTGTTGRQRVQKTDLLKLQIPLPP-LEEQKKIAEILRSIDEAI 194
+YI + R++L R+ + + K +L K + P EEQ+KI + +D+ I
Sbjct: 138 GYYINSNTYHRQLL--RLIQGSKVSSISKGNLQKTLVSFPKDFEEQQKIGSFFKQLDDTI 195
Query: 195 QAVDESIARLERLKKGTMERLLTRGINHTRFKTVELNGRKVEIPEEWDVVELGEVAERRN 254
+ L+ KKG ++++ + K EL R ++W+ +LG+ +
Sbjct: 196 ALHQRKLDLLKEQKKGYLQKMFPKNGE----KVPEL--RFAGFADDWEERKLGQYTKLIT 249
Query: 255 ESVNPAN---MGNIPFVGLEHIEPGNI----RLSQWGNSSEVKSSKSKFYPGDILYGKLR 307
+ P + +G++ FV +E+I G I ++ Q + + +K +S+ DIL+
Sbjct: 250 KGTTPKDKTGIGDVNFVKVENITNGKIYPINKIKQNEHDNYLK--RSRLEEKDILFSIAG 307
Query: 308 PYLDKAVIADFEGICSTD--IIVIKAKEDKTIPEYLIWVIHSKEFIEYAKKTMKGVNHPR 365
A++ +T+ + +I+ + T +LI + EY ++ P
Sbjct: 308 TLGRTAIVNKSILPANTNQALAIIRGYDFDT--NFLITSLAGNVVKEYIRRNPTVGAQPN 365
Query: 366 TSWKSIKQFQIPLPPLEEQKKIAEILRTIDEAIEAKRAKKEKLERMKKAVMEKL 419
S + + + P EEQ+KI + +DE I + K + L+ KK ++K+
Sbjct: 366 LSLEQVGNLLVNTPNAEEQQKIGSFFKQLDETIALHQRKLDLLKEQKKGFLQKM 419
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.136 0.381
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 153163782
Number of Sequences: 2977
Number of extensions: 6441480
Number of successful extensions: 21811
Number of sequences better than 1.0e-10: 24
Number of HSP's better than 0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 21693
Number of HSP's gapped (non-prelim): 53
length of query: 427
length of database: 189,106,746
effective HSP length: 59
effective length of query: 368
effective length of database: 153,796,013
effective search space: 56596932784
effective search space used: 56596932784
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 166 (69.1 bits)