BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2163 (rfA-like) DE:replication factor A related protein
(375 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75220 replication factor A related protein PAB2163 - Pyroc... 743 0.0
pir||H71202 hypothetical protein PH1893 - Pyrococcus horikoshii ... 657 0.0
pir||F64444 hypothetical protein MJ1159 - Methanococcus jannasch... 114 2e-24
pir||C69051 replication factor A related protein - Methanobacter... 87 2e-16
gb|AAG18754.1| (AE004980) replication A related protein; Rpa [Ha... 73 5e-12
>pir||B75220 replication factor A related protein PAB2163 - Pyrococcus abyssi
(strain Orsay) >gi|5457718|emb|CAB49209.1| (AJ248283)
replication factor A related protein [Pyrococcus abyssi]
Length = 375
Score = 743 bits (1898), Expect = 0.0
Identities = 375/375 (100%), Positives = 375/375 (100%)
Query: 1 MVNVKNRNILRYSKVVDMTVLTKDRIIEIIERKTGMSREEIEEEIRKIMEEDPYLSEQGA 60
MVNVKNRNILRYSKVVDMTVLTKDRIIEIIERKTGMSREEIEEEIRKIMEEDPYLSEQGA
Sbjct: 1 MVNVKNRNILRYSKVVDMTVLTKDRIIEIIERKTGMSREEIEEEIRKIMEEDPYLSEQGA 60
Query: 61 AALLAERLGIDLIEKEEVSLMRISELYPGMDPREVNVVGRVLKKYPPREYTRKDGSVGRV 120
AALLAERLGIDLIEKEEVSLMRISELYPGMDPREVNVVGRVLKKYPPREYTRKDGSVGRV
Sbjct: 61 AALLAERLGIDLIEKEEVSLMRISELYPGMDPREVNVVGRVLKKYPPREYTRKDGSVGRV 120
Query: 121 ASLIIYDDSGRARVVLWDAKVSEYYNKIEVGDVIKVLDAQVKESLSGLPELHINFRARII 180
ASLIIYDDSGRARVVLWDAKVSEYYNKIEVGDVIKVLDAQVKESLSGLPELHINFRARII
Sbjct: 121 ASLIIYDDSGRARVVLWDAKVSEYYNKIEVGDVIKVLDAQVKESLSGLPELHINFRARII 180
Query: 181 LNPDDPRVEMIPPLEEVRVATYTRKKIKDIEAGDRFVEVRGTIAKVYRVLTYDACPECKK 240
LNPDDPRVEMIPPLEEVRVATYTRKKIKDIEAGDRFVEVRGTIAKVYRVLTYDACPECKK
Sbjct: 181 LNPDDPRVEMIPPLEEVRVATYTRKKIKDIEAGDRFVEVRGTIAKVYRVLTYDACPECKK 240
Query: 241 KVDYDEGLGVWICPEHGEVQPIKMTILDFGLDDGTGYIRVTLFGDDAEELLGVSPEEIAE 300
KVDYDEGLGVWICPEHGEVQPIKMTILDFGLDDGTGYIRVTLFGDDAEELLGVSPEEIAE
Sbjct: 241 KVDYDEGLGVWICPEHGEVQPIKMTILDFGLDDGTGYIRVTLFGDDAEELLGVSPEEIAE 300
Query: 301 KIKELEESGLTTKEAARKLAEDEFYNIIGREIVVRGNVIEDRFLGLILRASSWEDVDYRR 360
KIKELEESGLTTKEAARKLAEDEFYNIIGREIVVRGNVIEDRFLGLILRASSWEDVDYRR
Sbjct: 301 KIKELEESGLTTKEAARKLAEDEFYNIIGREIVVRGNVIEDRFLGLILRASSWEDVDYRR 360
Query: 361 EIERIKEELEKLGVM 375
EIERIKEELEKLGVM
Sbjct: 361 EIERIKEELEKLGVM 375
>pir||H71202 hypothetical protein PH1893 - Pyrococcus horikoshii
>gi|3258332|dbj|BAA31015.1| (AP000007) 361aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 361
Score = 657 bits (1676), Expect = 0.0
Identities = 320/361 (88%), Positives = 347/361 (95%)
Query: 15 VVDMTVLTKDRIIEIIERKTGMSREEIEEEIRKIMEEDPYLSEQGAAALLAERLGIDLIE 74
++DM VLTK++IIEIIE KTGMSRE+IEEEIR+IMEEDP+LSEQGAAALLAERLGIDLIE
Sbjct: 1 MIDMVVLTKEKIIEIIEEKTGMSREKIEEEIRRIMEEDPHLSEQGAAALLAERLGIDLIE 60
Query: 75 KEEVSLMRISELYPGMDPREVNVVGRVLKKYPPREYTRKDGSVGRVASLIIYDDSGRARV 134
+EE SLM+IS+LYPGMDPREVN++GRVLKKYP REYT+KDGS+GRVASL+IYDDSGRARV
Sbjct: 61 REETSLMKISDLYPGMDPREVNIIGRVLKKYPVREYTKKDGSIGRVASLVIYDDSGRARV 120
Query: 135 VLWDAKVSEYYNKIEVGDVIKVLDAQVKESLSGLPELHINFRARIILNPDDPRVEMIPPL 194
VLWD KV+EYYNKIEVGDVIKVLDAQV+ESLSGLPELHINFRARIILNP+DPRV+ IPPL
Sbjct: 121 VLWDGKVAEYYNKIEVGDVIKVLDAQVRESLSGLPELHINFRARIILNPEDPRVDSIPPL 180
Query: 195 EEVRVATYTRKKIKDIEAGDRFVEVRGTIAKVYRVLTYDACPECKKKVDYDEGLGVWICP 254
EEVR+ATYTRKKI DIE GDRF+E+RGTIAKVYRVLTYDACPECKKKVDYD G GVWICP
Sbjct: 181 EEVRIATYTRKKIGDIEPGDRFIEIRGTIAKVYRVLTYDACPECKKKVDYDPGTGVWICP 240
Query: 255 EHGEVQPIKMTILDFGLDDGTGYIRVTLFGDDAEELLGVSPEEIAEKIKELEESGLTTKE 314
EHGEV+PIKMTILDFGLDDGTGYIRVTLFGDDAEELLGVS EEIAEKIKELE SGLTTKE
Sbjct: 241 EHGEVEPIKMTILDFGLDDGTGYIRVTLFGDDAEELLGVSSEEIAEKIKELENSGLTTKE 300
Query: 315 AARKLAEDEFYNIIGREIVVRGNVIEDRFLGLILRASSWEDVDYRREIERIKEELEKLGV 374
AARKLAE+EFY IIG+EIVVRGNVIEDRFLGLILRASSWE+VDYR+EIE IKEELEKLGV
Sbjct: 301 AARKLAEEEFYKIIGKEIVVRGNVIEDRFLGLILRASSWEEVDYRKEIENIKEELEKLGV 360
Query: 375 M 375
M
Sbjct: 361 M 361
>pir||F64444 hypothetical protein MJ1159 - Methanococcus jannaschii
>gi|1500014|gb|AAB99162.1| (U67558) replication factor A
related protein [Methanococcus jannaschii]
Length = 645
Score = 114 bits (282), Expect = 2e-24
Identities = 97/297 (32%), Positives = 161/297 (53%), Gaps = 43/297 (14%)
Query: 77 EVSLMRISELYP-GMDPREVNVVGRVLKKYPPREYTRKDGSVGRVASLIIYDDSGRARVV 135
E+ +I ++Y +D ++N++ +V++ Y E +D V +V +L++ D +GR R+
Sbjct: 389 ELKYCKIEDIYNRDVDWNDINLIAQVVEDYGVNEIEFED-KVRKVRNLLLEDGTGRIRLS 447
Query: 136 LWDAKVSEYYNKIEVGDVIKVLDAQVKESLSGLPELHINFRARIILNPDDPRVEMIPPLE 195
LWD ++E +I+ GD++++L A KE + +L I RII+NP E
Sbjct: 448 LWD-DLAEI--EIKEGDIVEILHAYAKERGDYI-DLVIGKYGRIIINP-----------E 492
Query: 196 EVRVATYTRKKIKDIEAGDRFVEVRGTIAKVYR-VLTYDACPECKKKVDYDEGLGVWICP 254
V + T RK I DIE G+ VEVRG + K+ L CP C+K+V +G ++ CP
Sbjct: 493 GVEIKT-NRKFIADIEDGET-VEVRGAVVKILSDTLFLYLCPNCRKRVVEIDG--IYNCP 548
Query: 255 EHGEVQPIKMTILDFGLDDGTGYIRVTLFGDDAEELLGVSPEEIAEKIKELEESGLTTKE 314
G+V+P ++ L+F +DDGTG + + E++L ++ EE+ K
Sbjct: 549 ICGDVEPEEILRLNFVVDDGTGTLLCRAYDRRVEKMLKMNREEL--------------KN 594
Query: 315 AARKLAEDEFYNIIGREIVVRGNV-IEDRFLGLILRASSWEDVDYRREIERIKEELE 370
++ EDE I+G E V+ GNV +E+ L +++R + DVD +EI RI EE+E
Sbjct: 595 LTIEMVEDE---ILGEEFVLYGNVRVENDELIMVVRRVN--DVDVEKEI-RILEEME 645
>pir||C69051 replication factor A related protein - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622495|gb|AAB85862.1| (AE000901) replication factor
A related protein [Methanobacterium thermoautotrophicum]
Length = 622
Score = 87.4 bits (213), Expect = 2e-16
Identities = 64/220 (29%), Positives = 109/220 (49%), Gaps = 31/220 (14%)
Query: 75 KEEVSLMRISELYPGMDP-REVNVVGRVLKKYPPREYTRKDGSVGRVASLIIYDDSGRAR 133
+E++ M+ISE+ D EV+VVGR+ PRE+ R+DG+ G V S+ + D++G+ R
Sbjct: 401 REQMRPMKISEILEMEDEGEEVDVVGRIFSLSDPREFEREDGT-GIVRSMELADETGKIR 459
Query: 134 VVLWDAKVSEYYNKIEVGDVIKVLDAQVKESLSGLPELHINFRARIILNPDDPRVEMIPP 193
+ LWD K + N +GD +++ +A+++ L + EL RI+ NP +E +P
Sbjct: 460 ISLWDEKAEKPMN---IGDAVRIENARIRLGLYSV-ELSAGRTTRIV-NPLPEDMEDLPS 514
Query: 194 LEEVRVATYTRKKIKDIEAGDRFVEVRGTIAKVYRVLTYDACPECKKKVDYDEGLGVWIC 253
EE+ Y KKI D+E DR + + + ++ +
Sbjct: 515 FEELEEMLYQTKKIADLEEDDRNIRIIARVVDLFEPREF--------------------- 553
Query: 254 PEHGEVQPIKMTILDFGLDDGTGYIRVTLFGDDAEELLGV 293
+ G+ P + +F D TG IR +L+ D AE+ L +
Sbjct: 554 -QRGDGTPGLVRTAEFA--DDTGSIRASLWDDAAEKPLSI 590
Score = 76.5 bits (185), Expect = 5e-13
Identities = 61/215 (28%), Positives = 104/215 (48%), Gaps = 31/215 (14%)
Query: 80 LMRISELYPGMDPREVNVVGRVLKKYPPREYTRKDGSVGRVASLIIYDDSGRARVVLWDA 139
L++I +L+ + R V V+G V K P E+ R DG+ G V S+ I DD+G ARV LWD
Sbjct: 289 LVKIGDLH---EMRNVTVMGLVTKVNDPVEFERNDGTTGSVKSIEIADDTGSARVTLWD- 344
Query: 140 KVSEYYNKIEVGDVIKVLDAQVK-ESLSGLPELHINFRARIILNPDDPRVEMIPPLEEVR 198
+ KI GD+I++ A V+ + + ++ NF RI LNP+ ++ LEE R
Sbjct: 345 --EDTRIKINKGDIIRISGANVEFDDFNQSYRINTNFNTRITLNPESDGA-LLKVLEEYR 401
Query: 199 VATYTRKKIKDIEAGDRFVEVRGTIAKVYRVLTYDACPECKKKVDYDEGLGVWICPEHGE 258
K + +E D EV + +++ + ++ + ++G G+
Sbjct: 402 EQMRPMKISEILEMEDEGEEV-DVVGRIFSL-------SDPREFEREDGTGI-------- 445
Query: 259 VQPIKMTILDFGLDDGTGYIRVTLFGDDAEELLGV 293
+ L D TG IR++L+ + AE+ + +
Sbjct: 446 -------VRSMELADETGKIRISLWDEKAEKPMNI 473
Score = 74.5 bits (180), Expect = 2e-12
Identities = 67/210 (31%), Positives = 98/210 (45%), Gaps = 23/210 (10%)
Query: 33 KTGMSREEIEEEIRKIMEE--------DPYLSEQGAAALLAERLGIDLIEKEEVSLMRIS 84
K +S EE EE I K EE D ++ +L E+ + L EK E + IS
Sbjct: 13 KDRISPEEFEELIEKKKEELGDIGFMDDLTIASTVVDDILKEKNTM-LSEKPEHRMDTIS 71
Query: 85 ELYPGMDPREVNVVGRVLKKYPPREYTRKDGSVGRVASLIIYDDSGRARVVLWDAKVSEY 144
+L G E V GRV+K PR +T + G G++A++II DD+G R V W + +
Sbjct: 72 KLEEGA---ETPVTGRVMKISSPRTFTTRKGREGKLANVIIADDTGELRAVFWTENI-KL 127
Query: 145 YNKIEVGDVIKVLDAQVKESLSGLPELHINFRARI-ILNPDDPRVEMIPPLEEVRVATYT 203
K GDVI++ D ++ G E H+ R+ + +L+P+D P E R
Sbjct: 128 LKKFREGDVIRIKDVNIRGGFGGRKEAHLMPRSTVEVLDPED-----YPEFPEYREEI-- 180
Query: 204 RKKIKDIEAGDRFVEVRGTIAKVYRVLTYD 233
I D+ D V V I V RV T++
Sbjct: 181 -TPIGDLVEDDE-VNVIARITGVSRVRTFE 208
>gb|AAG18754.1| (AE004980) replication A related protein; Rpa [Halobacterium sp.
NRC-1]
Length = 474
Score = 73.0 bits (176), Expect = 5e-12
Identities = 69/257 (26%), Positives = 118/257 (45%), Gaps = 48/257 (18%)
Query: 65 AERLGIDLIEKEEVSLMRISELYPGMD----PREVNVVGRVLKKYPPREYTRKDGSVGRV 120
A+ + +D E+ +V +I + Y D +V +VG VL R + R DGS GRV
Sbjct: 148 ADEVELDADEQIDV---QIQDEYDAADLSLGVSDVTLVGEVLDTEAVRTFDRDDGSEGRV 204
Query: 121 ASLIIYDDSGRARVVLWDAKVSEYYNKIEVGDVIKVLDAQVKESLSGLPELHINFRARII 180
++L++ D++GR RV LWD + ++ ++ G V++++D V+E G ELH+ +
Sbjct: 205 SNLVVGDETGRVRVTLWDDQ-ADTATDLDAGAVVEIVDGYVRER-EGSLELHVGDTGAVT 262
Query: 181 LNPDDPRVEMIPPLEEVRVATYTRKKIKDIEAGDRFVEVRGTIAKVYRVLTYDACPECKK 240
P D V +P + D D ++ G + V T+D
Sbjct: 263 PVP-DADVSFVPDTTSI-----------DSLEQDDTADIAGVVRSVDPTRTFDR------ 304
Query: 241 KVDYDEGLGVWICPEHGEVQPIKMTILDFGLDDGTGYIRVTLFGDDAEELLGVSP-EEIA 299
D+G G+V+ ++ L D TG IRV L+G+ A+ L + P +E+A
Sbjct: 305 ----DDG-------SEGQVRNVR-------LQDDTGDIRVALWGEKAD--LDIGPGDEVA 344
Query: 300 EKIKELEESGLTTKEAA 316
E+++ EA+
Sbjct: 345 ALDVEIQDGWQDDIEAS 361
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.140 0.390
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 140860725
Number of Sequences: 2977
Number of extensions: 6162054
Number of successful extensions: 22022
Number of sequences better than 1.0e-10: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 22000
Number of HSP's gapped (non-prelim): 16
length of query: 375
length of database: 189,106,746
effective HSP length: 58
effective length of query: 317
effective length of database: 154,394,500
effective search space: 48943056500
effective search space used: 48943056500
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 166 (69.1 bits)