BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB2207 (PAB2207) DE:POTASSIUM CHANNEL, PUTATIVE (205 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||E75211 probable potassium channel PAB2207 - Pyrococcus abys... 397 e-110 pir||E71247 hypothetical protein PH0236 - Pyrococcus horikoshii ... 372 e-102 pir||G69053 conserved hypothetical protein MTH1402 - Methanobact... 173 2e-42 gi|11499779 potassium channel, putative [Archaeoglobus fulgidus]... 161 6e-39 gb|AAG20247.1| (AE005100) potassium channel homolog; PchB [Halob... 141 6e-33 pir||D75211 probable potassium channel PAB2401 - Pyrococcus abys... 141 7e-33 pir||D71247 hypothetical protein PH0235 - Pyrococcus horikoshii ... 139 2e-32 pir||A72503 hypothetical protein APE1999 - Aeropyrum pernix (str... 75 8e-13 >pir||E75211 probable potassium channel PAB2207 - Pyrococcus abyssi (strain Orsay) >gi|5457649|emb|CAB49140.1| (AJ248283) POTASSIUM CHANNEL, PUTATIVE [Pyrococcus abyssi] Length = 205 Score = 397 bits (1009), Expect = e-110 Identities = 205/205 (100%), Positives = 205/205 (100%) Query: 1 MEEVEEFRYEPKSVKEIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQ 60 MEEVEEFRYEPKSVKEIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQ Sbjct: 1 MEEVEEFRYEPKSVKEIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQ 60 Query: 61 LMIHGVLAARNVKEAEQVIAIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGE 120 LMIHGVLAARNVKEAEQVIAIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGE Sbjct: 61 LMIHGVLAARNVKEAEQVIAIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGE 120 Query: 121 EIIGRIVVSPDSVIVGRTLGELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGR 180 EIIGRIVVSPDSVIVGRTLGELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGR Sbjct: 121 EIIGRIVVSPDSVIVGRTLGELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGR 180 Query: 181 GTRTSIEHLKEIARGAIRVVGNERA 205 GTRTSIEHLKEIARGAIRVVGNERA Sbjct: 181 GTRTSIEHLKEIARGAIRVVGNERA 205 >pir||E71247 hypothetical protein PH0236 - Pyrococcus horikoshii >gi|3256625|dbj|BAA29308.1| (AP000001) 205aa long hypothetical protein [Pyrococcus horikoshii] Length = 205 Score = 372 bits (944), Expect = e-102 Identities = 185/205 (90%), Positives = 201/205 (97%) Query: 1 MEEVEEFRYEPKSVKEIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQ 60 MEEVEEF+YEPKSVKEIFIEMKDTVELMVDLAYAS+LFGDKEIAEEVLELEERID+LNYQ Sbjct: 1 MEEVEEFKYEPKSVKEIFIEMKDTVELMVDLAYASLLFGDKEIAEEVLELEERIDLLNYQ 60 Query: 61 LMIHGVLAARNVKEAEQVIAIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGE 120 LM+H VLAARNVKEAEQVI I+Q+ANAIEDISNAAGDLAKMVLEG+ELHPVIKETI+EGE Sbjct: 61 LMMHSVLAARNVKEAEQVITILQIANAIEDISNAAGDLAKMVLEGVELHPVIKETILEGE 120 Query: 121 EIIGRIVVSPDSVIVGRTLGELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGR 180 EIIG+I V P+SVIVG+TLGELDLATNTGVWIIAVRRGKRWIFGP+E+FKIRAGD+LIGR Sbjct: 121 EIIGKIQVYPESVIVGKTLGELDLATNTGVWIIAVRRGKRWIFGPNENFKIRAGDVLIGR 180 Query: 181 GTRTSIEHLKEIARGAIRVVGNERA 205 GTRTSI+HLKEIARGAIRV+GNERA Sbjct: 181 GTRTSIDHLKEIARGAIRVIGNERA 205 >pir||G69053 conserved hypothetical protein MTH1402 - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622513|gb|AAB85879.1| (AE000902) conserved protein [Methanobacterium thermoautotrophicum] Length = 198 Score = 173 bits (433), Expect = 2e-42 Identities = 83/183 (45%), Positives = 131/183 (71%) Query: 12 KSVKEIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQLMIHGVLAARN 71 KSVK+I IEMK+ ELMVDLAY+++LF ++ AEEV++LE +++ LNY++ +LAAR+ Sbjct: 3 KSVKDILIEMKNLSELMVDLAYSALLFNSRDAAEEVIKLENKVNRLNYEIKKESLLAARS 62 Query: 72 VKEAEQVIAIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGEEIIGRIVVSPD 131 V++AE++ A++++ A E I+N+A D+A +VL+GI+ HPV K + E +EII R+ + Sbjct: 63 VEDAEELTALLEVGEAAESIANSAKDIADLVLKGIKPHPVFKMVMEESDEIIVRVTIEEG 122 Query: 132 SVIVGRTLGELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGRGTRTSIEHLKE 191 S + G++LG+L LAT TG+ +IA+RRG+ WI+GPD + + D LI +G E L Sbjct: 123 SELAGKSLGDLLLATRTGMRVIAIRRGESWIYGPDRNTVLAEKDTLIAKGNEAGAELLFA 182 Query: 192 IAR 194 +A+ Sbjct: 183 LAK 185 >gi|11499779 potassium channel, putative [Archaeoglobus fulgidus] >gi|7483920|pir||E69524 potassium channel homolog - Archaeoglobus fulgidus >gi|2648332|gb|AAB89059.1| (AE000954) potassium channel, putative [Archaeoglobus fulgidus] Length = 395 Score = 161 bits (403), Expect = 6e-39 Identities = 82/186 (44%), Positives = 127/186 (68%), Gaps = 1/186 (0%) Query: 11 PKSVKEIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQLMIHGVLAAR 70 P++VK++ +E+KDT ELMVDLAY++IL+ +++IAEEVLELE R+ L Q+ + +LAAR Sbjct: 2 PRTVKDLLVEIKDTTELMVDLAYSAILYNNEDIAEEVLELEGRVLDLLKQIRVVSILAAR 61 Query: 71 NVKEAEQVIAIIQMANAIEDISNAAGDLAKMVLEGIEL-HPVIKETIVEGEEIIGRIVVS 129 V+EAE+V +I+Q+ANA + IS+AA D++ +VL I L ++K + EE + ++ V Sbjct: 62 RVEEAEKVSSILQIANAGQKISSAAADISSLVLRNIRLSDEMVKLMLYHSEETVVKLEVP 121 Query: 130 PDSVIVGRTLGELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGRGTRTSIEHL 189 +S I G+TLGE+ L T TG+ +IA++RG WIF PD + GD+L +G ++ Sbjct: 122 ENSEIAGKTLGEVRLHTRTGMRVIAIKRGFSWIFNPDRDTMVYKGDLLFAKGDPAALPRF 181 Query: 190 KEIARG 195 E+ G Sbjct: 182 YEVVAG 187 Score = 123 bits (305), Expect = 2e-27 Identities = 64/179 (35%), Positives = 118/179 (65%), Gaps = 2/179 (1%) Query: 16 EIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQLMIHGVLAARNVKEA 75 ++ IE+K+ EL VDLAY+S+++G+ EIA EV+ LEE ID +++ + +R+ + Sbjct: 209 DLLIEVKNLSELSVDLAYSSLIYGNDEIALEVVYLEEVIDNTKFEIEKSVLKCSRHFNDE 268 Query: 76 E--QVIAIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGEEIIGRIVVSPDSV 133 + ++AI+++A E I++AA D+A+++L+ +E+ + KE + E +E++ I VS +S Sbjct: 269 QLMPLMAIVEIAYCSELIADAARDIAQVLLDKMEIPAIFKEAMRETDEVLTLITVSEESP 328 Query: 134 IVGRTLGELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGRGTRTSIEHLKEI 192 + G+TLGE + +NTG+ ++A++R WI P + +I +GD+LI +GTR L ++ Sbjct: 329 LNGKTLGEARVESNTGMHVVAIKRKNEWITKPTANTRIFSGDVLIAKGTREGESELLKL 387 >gb|AAG20247.1| (AE005100) potassium channel homolog; PchB [Halobacterium sp. NRC-1] Length = 418 Score = 141 bits (352), Expect = 6e-33 Identities = 75/191 (39%), Positives = 119/191 (62%), Gaps = 4/191 (2%) Query: 9 YEPKSVKEIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQLMIHGVLA 68 YEP S K++ +E+KDT EL+VDL+++++L G +A+EVL LE+R+D+L + + +LA Sbjct: 7 YEPASAKDLLVELKDTAELLVDLSFSAVLHGSDNVADEVLRLEKRMDVLQLRARMTLLLA 66 Query: 69 ARNVKEAEQVIAIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGEEIIGRIVV 128 AR+ +AE + ++ + A E +S+AAGD+AK+V E + L ++ + + E + R + Sbjct: 67 ARSPADAESLAPVLGVVAATEKMSDAAGDIAKVVREDVGLPDAMQGALPDAAESLVRGRL 126 Query: 129 SPDSVIVGRTLGELDLATNTGVWIIAVRRGK----RWIFGPDESFKIRAGDILIGRGTRT 184 DS RTLG++DL TNTGV +IAVRRG W+ P +R D+L+ RG T Sbjct: 127 VADSRYADRTLGDVDLETNTGVRVIAVRRGDGDAGDWLPNPGPETTLRPADVLLLRGPTT 186 Query: 185 SIEHLKEIARG 195 +E + E G Sbjct: 187 GVEGVYETVTG 197 Score = 121 bits (300), Expect = 7e-27 Identities = 67/194 (34%), Positives = 109/194 (55%), Gaps = 21/194 (10%) Query: 21 MKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQLMIHGVLAARNVKEAEQVIA 80 MK+ EL VDLAY ++LF D +AEEV+ELE +D L + + AA ++ + + Sbjct: 223 MKNMSELAVDLAYGAVLFDDDGLAEEVVELEAEVDALKSRFEAWTLRAATDIADPVSLRG 282 Query: 81 IIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGEEIIGRIVVSPDSVIVGRTLG 140 ++Q+A A E IS+AA ++++ VL G++ H V++E + +E++ R+ V + G TLG Sbjct: 283 LMQIATATEVISDAAVEISEGVLRGLDTHIVVQEAVEASDEVVVRVPVGAGGELPGTTLG 342 Query: 141 ELDLATNTGVWIIAVRRG---------------------KRWIFGPDESFKIRAGDILIG 179 + + T TG+ IIAVRRG W+ P +RAGD+L+ Sbjct: 343 DQRVKTRTGMRIIAVRRGATPDTGDVAAAGSRDGHRSPEDAWVIEPGPETTVRAGDVLLA 402 Query: 180 RGTRTSIEHLKEIA 193 +GTR+ + L+E+A Sbjct: 403 KGTRSGAQKLRELA 416 >pir||D75211 probable potassium channel PAB2401 - Pyrococcus abyssi (strain Orsay) >gi|5457648|emb|CAB49139.1| (AJ248283) POTASSIUM CHANNEL, PUTATIVE [Pyrococcus abyssi] Length = 191 Score = 141 bits (351), Expect = 7e-33 Identities = 71/184 (38%), Positives = 123/184 (66%), Gaps = 2/184 (1%) Query: 10 EPKSVKEIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQLMIHGVLAA 69 E + ++ IEMK+ LM+DLA +S+++ +EIAEEV LEE++D L Y++ + AA Sbjct: 3 ELEEIRNCLIEMKNISSLMIDLALSSVMYNSEEIAEEVYLLEEKMDELTYKVKKLALEAA 62 Query: 70 RNVKEAEQVIAIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGEEIIGRIVVS 129 + + E E ++++I+MAN E IS++A ++A +VL +E HP+I++ + + +E IGR+ V Sbjct: 63 KKIDEPESMLSVIEMANINEQISDSAYEIADIVLRDVEPHPIIRKIMHDVDEEIGRVKVK 122 Query: 130 PDSVIVGRTLGELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGRGTRTSIEHL 189 S+++G++L +L L + GV IIA++RG ++I+ P S I+ GD LI G ++ L Sbjct: 123 KGSILIGKSLRQLKLPSKIGVRIIAIKRGNKYIYDPQSSEVIQEGDTLIAVG--AGVDRL 180 Query: 190 KEIA 193 +E+A Sbjct: 181 RELA 184 >pir||D71247 hypothetical protein PH0235 - Pyrococcus horikoshii >gi|3256624|dbj|BAA29307.1| (AP000001) 190aa long hypothetical protein [Pyrococcus horikoshii] Length = 190 Score = 139 bits (348), Expect = 2e-32 Identities = 76/194 (39%), Positives = 127/194 (65%), Gaps = 9/194 (4%) Query: 1 MEEVEEFRYEPKSVKEIFIEMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQ 60 M+E+EE R IEM++ LM+DLA +S+++ +EIAEEV LEE++D L + Sbjct: 1 MKELEEIR-------SCLIEMRNLSSLMIDLALSSVMYNSEEIAEEVYLLEEKMDELTLK 53 Query: 61 LMIHGVLAARNVKEAEQVIAIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGE 120 + + AA+ + + E ++++I+MAN E IS++A ++A +VL +E HP+I++ + + E Sbjct: 54 VKKLALEAAKKIDDPESMLSVIEMANINEQISDSAYEIADLVLRDVEPHPIIRKIMHDVE 113 Query: 121 EIIGRIVVSPDSVIVGRTLGELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGR 180 E IGR+ V+ S++VG++L +L L + GV IIA++RG R+I+ P + IR GD LI Sbjct: 114 EEIGRVRVNKGSILVGKSLRQLKLPSKIGVRIIAIKRGNRYIYNPPSNEIIREGDTLIAV 173 Query: 181 GTRTSIEHLKEIAR 194 G I+ L+E+A+ Sbjct: 174 G--AGIDKLRELAK 185 >pir||A72503 hypothetical protein APE1999 - Aeropyrum pernix (strain K1) >gi|5105697|dbj|BAA81009.1| (AP000063) 382aa long hypothetical protein [Aeropyrum pernix] Length = 382 Score = 74.5 bits (180), Expect = 8e-13 Identities = 56/174 (32%), Positives = 87/174 (49%), Gaps = 16/174 (9%) Query: 20 EMKDTVELMVDLAYASILFGDKEIAEEVLELEERIDILNYQLMIHGVLAARNVKEAEQVI 79 E + +M+DL+ S+ FGD+ A E L LE+ +D + + + LA R Sbjct: 17 EARRLTSIMIDLSIYSVAFGDRLAALETLMLEDDVDEIIREAVGLLSLAVRGPGHTGLAK 76 Query: 80 AIIQMANAIEDISNAAGDLAKMVLEGIELHPVIKETIVEGEEIIGRIVVSPDSVIVGRTL 139 I+++A A + S+AAGDLA++VL G H + + E++ + + L Sbjct: 77 GIVELAAAFDRASDAAGDLAQLVLRGYPPHKYVTAAAICCGEVVAALKAE-------KPL 129 Query: 140 GELDLATNTGVWIIAVRRGKRWIFGPDESFKIRAGDILIGRGTRTSIEHLKEIA 193 LD+ V ++ +RRG R + P E F IR GD+LI RG S+E L E A Sbjct: 130 DRLDI-----VDVLILRRGGRALLSP-EDFSIREGDVLIVRG---SLEALLEAA 174 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.319 0.139 0.378 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 68174835 Number of Sequences: 2977 Number of extensions: 2617003 Number of successful extensions: 8109 Number of sequences better than 1.0e-10: 8 Number of HSP's better than 0.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 8092 Number of HSP's gapped (non-prelim): 11 length of query: 205 length of database: 189,106,746 effective HSP length: 57 effective length of query: 148 effective length of database: 154,992,987 effective search space: 22938962076 effective search space used: 22938962076 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 163 (67.9 bits)