BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2213 (PAB2213) DE:sterol biosynthesis methyltransferase
related
(227 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75210 sterol biosynthesis methyltransferase related PAB221... 462 e-129
pir||C71246 hypothetical protein PH0226 - Pyrococcus horikoshii ... 404 e-112
pir||G75136 menaquinone biosynthesis methlytransferase related P... 86 3e-16
pir||A71001 hypothetical protein PH1305 - Pyrococcus horikoshii ... 85 9e-16
pir||T42375 probable 24-sterol C-methyltransferase (EC 2.1.1.41)... 76 4e-13
gb|AAB31378.1| (S72460) putative S-adenosylmethionine-dependent ... 76 4e-13
sp|O14321|ERG6_SCHPO PROBABLE DELTA(24)-STEROL C-METHYLTRANSFERA... 76 4e-13
emb|CAA37826.1| (X53830) ORF (AA 1 - 258) [Saccharomyces cerevis... 76 4e-13
gi|6323635 S-adenoslymethionine: delta 24-methyltransferase; Erg... 76 4e-13
pir||T33885 hypothetical protein H14E04.1 - Caenorhabditis elega... 73 3e-12
pir||B72316 conserved hypothetical protein - Thermotoga maritima... 72 7e-12
sp|P49016|GRCB_LACLA PROBABLE MENAQUINONE BIOSYNTHESIS METHYLTRA... 70 2e-11
pir||B48653 hypothetical protein 2 (pip 3' region) - Lactococcus... 70 2e-11
gb|AAF24830.1|AC012679_1 (AC012679) putative S-adenosyl-methioni... 70 3e-11
gb|AAG12776.1|AC079676_1 (AC079676) phosphoethanolamine N-methyl... 70 3e-11
sp|P54458|YQEM_BACSU HYPOTHETICAL 28.3 KD PROTEIN IN AROD-COMER ... 69 4e-11
pir||A81324 probable cyclopropane-fatty-acyl-phospholipid syntha... 69 4e-11
pir||T03845 probable 24-sterol C-methyltransferase (EC 2.1.1.41)... 69 6e-11
pir||E69758 hypothetical protein ycgJ - Bacillus subtilis >gi|26... 69 6e-11
gb|AAD28459.1|AF127374_14 (AF127374) MitM [Streptomyces lavendulae] 69 6e-11
>pir||C75210 sterol biosynthesis methyltransferase related PAB2213 - Pyrococcus
abyssi (strain Orsay) >gi|5457639|emb|CAB49130.1|
(AJ248283) sterol biosynthesis methyltransferase related
[Pyrococcus abyssi]
Length = 227
Score = 462 bits (1177), Expect = e-129
Identities = 227/227 (100%), Positives = 227/227 (100%)
Query: 1 MGFAEYYRAFPTYTDINSQEYRDRLENLEPLLMKYMKRRGKVLDLACGVGGFSFLLEDYG 60
MGFAEYYRAFPTYTDINSQEYRDRLENLEPLLMKYMKRRGKVLDLACGVGGFSFLLEDYG
Sbjct: 1 MGFAEYYRAFPTYTDINSQEYRDRLENLEPLLMKYMKRRGKVLDLACGVGGFSFLLEDYG 60
Query: 61 FEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDAKKLPFEDNNFDYVIFIDSLVHFSPLE 120
FEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDAKKLPFEDNNFDYVIFIDSLVHFSPLE
Sbjct: 61 FEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDAKKLPFEDNNFDYVIFIDSLVHFSPLE 120
Query: 121 LNQVFKEVKRVLKPTGKFIIYFTDMRELLPRLRESLVVGEKYWINKIIPDQEEKTVVIEF 180
LNQVFKEVKRVLKPTGKFIIYFTDMRELLPRLRESLVVGEKYWINKIIPDQEEKTVVIEF
Sbjct: 121 LNQVFKEVKRVLKPTGKFIIYFTDMRELLPRLRESLVVGEKYWINKIIPDQEEKTVVIEF 180
Query: 181 KSEENSFRVRFNVWGKTGVELLAKLYFNKEAQEKVGNYSYFIVYTPK 227
KSEENSFRVRFNVWGKTGVELLAKLYFNKEAQEKVGNYSYFIVYTPK
Sbjct: 181 KSEENSFRVRFNVWGKTGVELLAKLYFNKEAQEKVGNYSYFIVYTPK 227
>pir||C71246 hypothetical protein PH0226 - Pyrococcus horikoshii
>gi|3256615|dbj|BAA29298.1| (AP000001) 227aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 227
Score = 404 bits (1027), Expect = e-112
Identities = 190/227 (83%), Positives = 211/227 (92%)
Query: 1 MGFAEYYRAFPTYTDINSQEYRDRLENLEPLLMKYMKRRGKVLDLACGVGGFSFLLEDYG 60
MGF EYYR FPTYTDINSQEYR R+E LEPLLMKYMK+RGKVLDLACGVGGFSFLLEDYG
Sbjct: 1 MGFKEYYRVFPTYTDINSQEYRSRIETLEPLLMKYMKKRGKVLDLACGVGGFSFLLEDYG 60
Query: 61 FEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDAKKLPFEDNNFDYVIFIDSLVHFSPLE 120
FEVVG+DISE+MI KA+ YAK + SNVEFI+GDA+KL FED FDYVIFIDS+VHF PLE
Sbjct: 61 FEVVGVDISEDMIRKAREYAKSRESNVEFIVGDARKLSFEDKTFDYVIFIDSIVHFEPLE 120
Query: 121 LNQVFKEVKRVLKPTGKFIIYFTDMRELLPRLRESLVVGEKYWINKIIPDQEEKTVVIEF 180
LNQVFKEV+RVLKP+GKFI+YFTD+RELLPRL+ESLVVG+KYWI+K+IPDQEE+TVVIEF
Sbjct: 121 LNQVFKEVRRVLKPSGKFIMYFTDLRELLPRLKESLVVGQKYWISKVIPDQEERTVVIEF 180
Query: 181 KSEENSFRVRFNVWGKTGVELLAKLYFNKEAQEKVGNYSYFIVYTPK 227
KSE++SFRVRFNVWGKTGVELLAKLYF KEA+EKVGNYSY VY PK
Sbjct: 181 KSEQDSFRVRFNVWGKTGVELLAKLYFTKEAEEKVGNYSYLTVYNPK 227
>pir||G75136 menaquinone biosynthesis methlytransferase related PAB1765 -
Pyrococcus abyssi (strain Orsay)
>gi|5458319|emb|CAB49808.1| (AJ248285) MENAQUINONE
BIOSYNTHESIS METHLYTRANSFERASE related [Pyrococcus
abyssi]
Length = 248
Score = 86.2 bits (210), Expect = 3e-16
Identities = 63/235 (26%), Positives = 110/235 (46%), Gaps = 42/235 (17%)
Query: 5 EYYRAFPTYTDINSQEYRD----RLENLEPLLMKYMKRRGK-VLDLACGVGGFSFLLEDY 59
E Y Y D+ ++ ++ ++ +E L + +R K +LDLACG G + L
Sbjct: 3 ELYTVLAEYYDVIYRKRKELVKKEIDFVEDLFRREAEREVKRILDLACGTGTPTLELAKR 62
Query: 60 GFEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDAKKLPFEDNNFDYVIFIDSLVHFSPL 119
G+EV+GLD+ EEM+ A+ ++++ VEFI G+A ++ FE+ +F S+ +F
Sbjct: 63 GYEVIGLDLHEEMLQVARRKSEKEGIKVEFIQGNALEIDFEEEFDAITMFFSSITYFDDY 122
Query: 120 ELNQVFKEVKRVLKPTGKFIIYF-------------------------TDMRELLPRLRE 154
+ Q+F +K+ LKP G F+ F TD RE+ P ++
Sbjct: 123 SIQQLFNSIKQALKPGGIFVADFPCWFYSGKDYPIIWDEQNGDERLVITDWREIEPAFQK 182
Query: 155 SLVVGEKYWINKIIPDQEEKTVVIEFKSEENSFRVRFNVWGKTGVELLAKLYFNK 209
+ K + I PD KT +++ + N++ + LLA+ +F K
Sbjct: 183 ---MRFKRLVQIIKPDGSVKTFMVDDE---------LNIYTPREMRLLAEKHFRK 225
>pir||A71001 hypothetical protein PH1305 - Pyrococcus horikoshii
>gi|3257726|dbj|BAA30409.1| (AP000006) 252aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 252
Score = 84.7 bits (206), Expect = 9e-16
Identities = 72/228 (31%), Positives = 110/228 (47%), Gaps = 29/228 (12%)
Query: 7 YRAFPTYTDINSQEYRDRLENL-------EPLLMKYMKRR-GKVLDLACGVGGFSFLLED 58
Y + + YR R+E + E + + KR +VLDLACG G + L +
Sbjct: 2 YELYTLLAEYYDTIYRRRIERVKAEIDFVEEIFKEDAKREVRRVLDLACGTGIPTLELAE 61
Query: 59 YGFEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDAKKLPFEDNNFDYV-IFIDSLVHFS 117
G+EVVGLD+ EEM+ A+ AKE++ +EF+ GD ++ F+ N FD V +F ++++F
Sbjct: 62 RGYEVVGLDLHEEMLRVARRKAKERNLKIEFLQGDVLEIAFK-NEFDAVTMFFSTIMYFD 120
Query: 118 PLELNQVFKEVKRVLKPTGKFIIYFTDMRELLPRLRESLVV-----GEKYWINKIIPDQE 172
+L ++F +V LKP G FI TD R+ VV GE+ + + E
Sbjct: 121 EEDLRKLFSKVAEALKPGGVFI---TDFPCWFYGGRDGPVVWNEQKGEEKLVIMDWREVE 177
Query: 173 EKTVVIEFK---------SEENSFRV--RFNVWGKTGVELLAKLYFNK 209
+ FK E +F V N++ V LLA+ YF K
Sbjct: 178 PAVQKLRFKRLVQILRPNGEVKAFLVDDELNIYTPREVRLLAEKYFEK 225
>pir||T42375 probable 24-sterol C-methyltransferase (EC 2.1.1.41) - fission
yeast (Schizosaccharomyces pombe) (fragment)
>gi|1749470|dbj|BAA13793.1| (D89131) similar to
Saccharomyces cerevisiae delta (24)-sterol
C-methyltransferase, SWISS-PROT Accession number P25087
[Schizosaccharomyces pombe]
Length = 324
Score = 75.7 bits (183), Expect = 4e-13
Identities = 48/126 (38%), Positives = 74/126 (58%), Gaps = 9/126 (7%)
Query: 36 MKRRGKVLDLACGVGGFSFLLEDY-GFEVVGLDISEEMISKAKMYAKEKSSNVE--FIIG 92
+K +VLD+ CGVGG + + ++ G +VGL+ ++ IS+ YA +++ + + F+ G
Sbjct: 68 IKPGSRVLDVGCGVGGPAREITEFTGCNLVGLNNNDYQISRCNNYAVKRNLDKKQVFVKG 127
Query: 93 DAKKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFIIY----FTDMREL 148
D +PFEDN FDYV I++ VH LE V+ E+ RVLKP G F +Y D
Sbjct: 128 DFMHMPFEDNTFDYVYAIEATVHAPSLE--GVYGEIFRVLKPGGVFGVYEWVMSDDYDSS 185
Query: 149 LPRLRE 154
+P+ RE
Sbjct: 186 IPKHRE 191
>gb|AAB31378.1| (S72460) putative S-adenosylmethionine-dependent
methyltransferase=LIS1/ERG6 [Saccharomyces cerevisiae,
Peptide, 384 aa]
Length = 384
Score = 75.7 bits (183), Expect = 4e-13
Identities = 44/109 (40%), Positives = 67/109 (61%), Gaps = 5/109 (4%)
Query: 36 MKRRGKVLDLACGVGGFSFLLEDY-GFEVVGLDISEEMISKAKMYAKEK--SSNVEFIIG 92
++R VLD+ CGVGG + + + G V+GL+ ++ I+KAK YAK+ S ++F+ G
Sbjct: 117 IQRGDLVLDVGCGVGGPAREIARFTGCNVIGLNNNDYQIAKAKYYAKKYNLSDQMDFVKG 176
Query: 93 DAKKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFIIY 141
D K+ FE+N FD V I++ H LE V+ E+ +VLKP G F +Y
Sbjct: 177 DFMKMDFEENTFDKVYAIEATCHAPKLE--GVYSEIYKVLKPGGTFAVY 223
>sp|O14321|ERG6_SCHPO PROBABLE DELTA(24)-STEROL C-METHYLTRANSFERASE
>gi|7493435|pir||T39579 sterol methyltransferase -
fission yeast (Schizosaccharomyces pombe)
>gi|2467267|emb|CAB16897.1| (Z99759) putative
delta-sterol c-methyltransferase [Schizosaccharomyces
pombe]
Length = 378
Score = 75.7 bits (183), Expect = 4e-13
Identities = 48/126 (38%), Positives = 74/126 (58%), Gaps = 9/126 (7%)
Query: 36 MKRRGKVLDLACGVGGFSFLLEDY-GFEVVGLDISEEMISKAKMYAKEKSSNVE--FIIG 92
+K +VLD+ CGVGG + + ++ G +VGL+ ++ IS+ YA +++ + + F+ G
Sbjct: 122 IKPGSRVLDVGCGVGGPAREITEFTGCNLVGLNNNDYQISRCNNYAVKRNLDKKQVFVKG 181
Query: 93 DAKKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFIIY----FTDMREL 148
D +PFEDN FDYV I++ VH LE V+ E+ RVLKP G F +Y D
Sbjct: 182 DFMHMPFEDNTFDYVYAIEATVHAPSLE--GVYGEIFRVLKPGGVFGVYEWVMSDDYDSS 239
Query: 149 LPRLRE 154
+P+ RE
Sbjct: 240 IPKHRE 245
>emb|CAA37826.1| (X53830) ORF (AA 1 - 258) [Saccharomyces cerevisiae]
Length = 258
Score = 75.7 bits (183), Expect = 4e-13
Identities = 44/109 (40%), Positives = 67/109 (61%), Gaps = 5/109 (4%)
Query: 36 MKRRGKVLDLACGVGGFSFLLEDY-GFEVVGLDISEEMISKAKMYAKEK--SSNVEFIIG 92
++R VLD+ CGVGG + + + G V+GL+ ++ I+KAK YAK+ S ++F+ G
Sbjct: 117 IQRGDLVLDVGCGVGGPAREIARFTGCNVIGLNNNDYQIAKAKYYAKKYNLSDQMDFVKG 176
Query: 93 DAKKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFIIY 141
D K+ FE+N FD V I++ H LE V+ E+ +VLKP G F +Y
Sbjct: 177 DFMKMDFEENTFDKVYAIEATCHAPKLE--GVYSEIYKVLKPGGTFAVY 223
>gi|6323635 S-adenoslymethionine: delta 24-methyltransferase; Erg6p
[Saccharomyces cerevisiae]
>gi|462024|sp|P25087|ERG6_YEAST DELTA(24)-STEROL
C-METHYLTRANSFERASE >gi|630333|pir||S42003 24-sterol
C-methyltransferase (EC 2.1.1.41) - yeast
(Saccharomyces cerevisiae) >gi|396515|emb|CAA52308.1|
(X74249) S-adenosyl-methionine:delta-24-sterol-C-
methyltransferase [Saccharomyces cerevisiae]
>gi|854482|emb|CAA89944.1| (Z49810) Erg6p [Saccharomyces
cerevisiae]
Length = 383
Score = 75.7 bits (183), Expect = 4e-13
Identities = 44/109 (40%), Positives = 67/109 (61%), Gaps = 5/109 (4%)
Query: 36 MKRRGKVLDLACGVGGFSFLLEDY-GFEVVGLDISEEMISKAKMYAKEK--SSNVEFIIG 92
++R VLD+ CGVGG + + + G V+GL+ ++ I+KAK YAK+ S ++F+ G
Sbjct: 117 IQRGDLVLDVGCGVGGPAREIARFTGCNVIGLNNNDYQIAKAKYYAKKYNLSDQMDFVKG 176
Query: 93 DAKKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFIIY 141
D K+ FE+N FD V I++ H LE V+ E+ +VLKP G F +Y
Sbjct: 177 DFMKMDFEENTFDKVYAIEATCHAPKLE--GVYSEIYKVLKPGGTFAVY 223
>pir||T33885 hypothetical protein H14E04.1 - Caenorhabditis elegans
>gi|4226092|gb|AAD12813.1| (AF125448) contains
similarity to methyltransferases [Caenorhabditis
elegans]
Length = 366
Score = 73.0 bits (176), Expect = 3e-12
Identities = 39/101 (38%), Positives = 58/101 (56%), Gaps = 4/101 (3%)
Query: 43 LDLACGVGGFSFLLEDYGFEVVGLDIS--EEMISKAKMYAKEKSSNVEFIIGDAKKLPFE 100
LD+ CG+GG + D+G ++ G+ I+ E I K S + + D +K+PFE
Sbjct: 100 LDIGCGIGGVMLDIADFGAKLTGVTIAPNEAEIGNEKFANMGISDRCKIVAADCQKMPFE 159
Query: 101 DNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFIIY 141
D+ FD I SL + L++V KE++RVLKP GKFI+Y
Sbjct: 160 DSTFDVAYAIYSLKYIP--NLDKVMKEIQRVLKPGGKFIVY 198
>pir||B72316 conserved hypothetical protein - Thermotoga maritima (strain MSB8)
>gi|4981476|gb|AAD36019.1|AE001757_12 (AE001757)
conserved hypothetical protein [Thermotoga maritima]
Length = 254
Score = 71.8 bits (173), Expect = 7e-12
Identities = 57/177 (32%), Positives = 90/177 (50%), Gaps = 11/177 (6%)
Query: 26 ENLEPLLMKYMKRRGKVLDLACGVGGFSFLLEDYGFEVVGLDISEEMISKAKMYAKEKSS 85
+N +L + R KVLD+ACG G F+ + GF+VVG+D+S EM+ A+ AKE+S
Sbjct: 26 KNFAKILENFHIRGKKVLDVACGEGTFAVEIAKQGFKVVGVDLSSEMLKFARKRAKEESI 85
Query: 86 NVEFIIGDAKKLPFEDNNFDYV-IFIDSLVHFSPL-ELNQVFKEVKRVLKPTGKFIIYFT 143
+V F+ D ++L F + FD V + DSL + +L + F++V LK G F+
Sbjct: 86 SVVFLKKDMRELDFHE-EFDIVTCWFDSLNYLLDYKDLKKTFEKVHEALKTGGAFLFDMN 144
Query: 144 DMRELLPRLRESLVVGEK-----YWINKIIPDQEEKTV---VIEFKSEENSFRVRFN 192
+ LL +E V ++ + + I D EE V F+ +E RF+
Sbjct: 145 TVYGLLMANQEGPVYIQQDGKDIFEVQTIEFDLEESVATFYVTVFERKEEELWERFD 201
>sp|P49016|GRCB_LACLA PROBABLE MENAQUINONE BIOSYNTHESIS METHYLTRANSFERASE (GERC2 PROTEIN
HOMOLOG) >gi|410740|gb|AAA03166.1| (L14679) putative
[Lactococcus lactis]
Length = 252
Score = 70.2 bits (169), Expect = 2e-11
Identities = 50/115 (43%), Positives = 68/115 (58%), Gaps = 11/115 (9%)
Query: 34 KYMKRRG-----KVLDLACGVGGFSF-LLEDYG--FEVVGLDISEEMISKAKMYAKEKSS 85
K MKR G +LDL CG G ++F L E G +V+GLD SE M+ AK KE++
Sbjct: 44 KTMKRMGDLTGLSILDLCCGTGDWTFDLSESVGPSGKVIGLDFSENMLEIAKAKLKEEAK 103
Query: 86 -NVEFIIGDAKKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFI 139
N+EF+ G+A LPFE +FD V+ I + +P L V KE+ RVLKP G+ +
Sbjct: 104 KNIEFLQGNAMALPFEKGSFD-VVTIGYGLRNTPDYLT-VLKEIFRVLKPGGRVV 156
>pir||B48653 hypothetical protein 2 (pip 3' region) - Lactococcus lactis subsp.
lactis (strain C2)
Length = 252
Score = 70.2 bits (169), Expect = 2e-11
Identities = 50/115 (43%), Positives = 68/115 (58%), Gaps = 11/115 (9%)
Query: 34 KYMKRRG-----KVLDLACGVGGFSF-LLEDYG--FEVVGLDISEEMISKAKMYAKEKSS 85
K MKR G +LDL CG G ++F L E G +V+GLD SE M+ AK KE++
Sbjct: 44 KTMKRMGDLTGLSILDLCCGTGDWTFDLSESVGPSGKVIGLDFSENMLEIAKAKLKEEAK 103
Query: 86 -NVEFIIGDAKKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFI 139
N+EF+ G+A LPFE +FD V+ I + +P L V KE+ RVLKP G+ +
Sbjct: 104 KNIEFLQGNAMALPFEKGSFD-VVTIGYGLRNTPDYLT-VLKEIFRVLKPGGRVV 156
>gb|AAF24830.1|AC012679_1 (AC012679) putative
S-adenosyl-methionine-sterol-C-methyltransferase, 5'
partial; 1-1344 [Arabidopsis thaliana]
Length = 295
Score = 69.9 bits (168), Expect = 3e-11
Identities = 39/106 (36%), Positives = 61/106 (56%), Gaps = 3/106 (2%)
Query: 36 MKRRGKVLDLACGVGGFSFLL-EDYGFEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDA 94
+K KVLD+ CG+GG F + E++ +VVG+D+S MIS A +A +VEF + D
Sbjct: 84 LKPGQKVLDVGCGIGGGDFYMAENFDVDVVGIDLSVNMISFALEHAIGLKCSVEFEVADC 143
Query: 95 KKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFII 140
K + DN FD + D+++H + +F+ + LKP GK +I
Sbjct: 144 TKKEYPDNTFDVIYSRDTILHIQ--DKPALFRRFYKWLKPGGKVLI 187
>gb|AAG12776.1|AC079676_1 (AC079676) phosphoethanolamine N-methyltransferase, putative;
6854-3993 [Arabidopsis thaliana]
Length = 555
Score = 69.9 bits (168), Expect = 3e-11
Identities = 39/106 (36%), Positives = 61/106 (56%), Gaps = 3/106 (2%)
Query: 36 MKRRGKVLDLACGVGGFSFLL-EDYGFEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDA 94
+K KVLD+ CG+GG F + E++ +VVG+D+S MIS A +A +VEF + D
Sbjct: 344 LKPGQKVLDVGCGIGGGDFYMAENFDVDVVGIDLSVNMISFALEHAIGLKCSVEFEVADC 403
Query: 95 KKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFII 140
K + DN FD + D+++H + +F+ + LKP GK +I
Sbjct: 404 TKKEYPDNTFDVIYSRDTILHIQ--DKPALFRRFYKWLKPGGKVLI 447
>sp|P54458|YQEM_BACSU HYPOTHETICAL 28.3 KD PROTEIN IN AROD-COMER INTERGENIC REGION
>gi|7474691|pir||A69952 conserved hypothetical protein
yqeM - Bacillus subtilis >gi|1303794|dbj|BAA12450.1|
(D84432) YqeM [Bacillus subtilis]
>gi|2635007|emb|CAB14503.1| (Z99117) similar to
hypothetical proteins [Bacillus subtilis]
Length = 247
Score = 69.1 bits (166), Expect = 4e-11
Identities = 42/103 (40%), Positives = 61/103 (58%), Gaps = 3/103 (2%)
Query: 36 MKRRGKVLDLACGVGGFSFLLEDYGFEVVGLDISEEMISKAKMYAKEKSSNVEFIIGDAK 95
+ +G++LDLACG G S L + GFEV G+D+SEEM+S A+ S + F+ D +
Sbjct: 30 LPEKGRILDLACGTGEISIRLAEKGFEVTGIDLSEEMLSFAQQKV-SSSQPILFLQQDMR 88
Query: 96 KLPFEDNNFD-YVIFIDSLVHF-SPLELNQVFKEVKRVLKPTG 136
++ D FD VI DSL + + ++ + FK V RVLKP G
Sbjct: 89 EITGFDGQFDAVVICCDSLNYLKTKNDVIETFKSVFRVLKPEG 131
>pir||A81324 probable cyclopropane-fatty-acyl-phospholipid synthase (EC
2.1.1.79) Cj1183c [imported] - Campylobacter jejuni
(strain NCTC 11168) >gi|6968616|emb|CAB73437.1|
(AL139077) putative cyclopropane-fatty-acyl-phospholipid
synthase [Campylobacter jejuni]
Length = 387
Score = 69.1 bits (166), Expect = 4e-11
Identities = 45/133 (33%), Positives = 69/133 (51%), Gaps = 5/133 (3%)
Query: 13 YTDINSQEYRDRLENLEPLLMKYMKRRG-KVLDLACGVGGFSFLL-EDYGFEVVGLDISE 70
+ + N+ Y ++ +E L K + G K+LD+ CG G S + + YG +VVG+ ISE
Sbjct: 135 FKEPNNTLYEAQINKIEHTLKKLDLKEGEKLLDIGCGWGWLSIMAAQKYGVKVVGITISE 194
Query: 71 EMISKAKMYAKEKS--SNVEFIIGDAKKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEV 128
E KAK KE +E + + + L FE N FD V+ + H L F +V
Sbjct: 195 EQCKKAKERVKELGLEDKIEIRLQNYQDLEFE-NYFDKVVSVGMFEHVGKENLGLYFMKV 253
Query: 129 KRVLKPTGKFIIY 141
K+VLKP G +++
Sbjct: 254 KQVLKPGGSMLLH 266
>pir||T03845 probable 24-sterol C-methyltransferase (EC 2.1.1.41) - common
tobacco (fragment) >gi|2246452|gb|AAB62807.1| (U71107)
S-adenosyl-methionine-sterol-C-methyltransferase homolog
[Nicotiana tabacum]
Length = 352
Score = 68.7 bits (165), Expect = 6e-11
Identities = 38/109 (34%), Positives = 63/109 (56%), Gaps = 5/109 (4%)
Query: 36 MKRRGKVLDLACGVGG-FSFLLEDYGFEVVGLDISEEMISKAKMYAKEKS--SNVEFIIG 92
+K +VLD CGVGG + G +VG+ I+E +++A+ + K+ S E + G
Sbjct: 116 IKPGARVLDAGCGVGGPMRAIAAHSGANIVGITINEYQVNRARAHNKKAGLDSQCEVVCG 175
Query: 93 DAKKLPFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFIIY 141
+ ++PF+DN+FD V I++ H LE +V+ E+ RVLKP ++ Y
Sbjct: 176 NFLQMPFDDNSFDGVYSIEATCHAPKLE--EVYSEIYRVLKPGSMYVSY 222
>pir||E69758 hypothetical protein ycgJ - Bacillus subtilis
>gi|2632602|emb|CAB12110.1| (Z99105) ycgJ [Bacillus
subtilis]
Length = 228
Score = 68.7 bits (165), Expect = 6e-11
Identities = 37/101 (36%), Positives = 59/101 (57%), Gaps = 3/101 (2%)
Query: 41 KVLDLACGVGGFSFLLEDYGFEVVGLDISEEMISKAKMYAKEKS-SNVEFIIGDAKKLPF 99
+VLD+ G G + Y E +G+D ++EM+ A +A+EK NV F G A+ LPF
Sbjct: 15 RVLDIGAGAGHTALAFSPYVQECIGVDATKEMVEVASSFAQEKGVENVRFQQGTAESLPF 74
Query: 100 EDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFII 140
D++FD + + HFS ++ + +EV RVLK G+F++
Sbjct: 75 PDDSFDIITCRYAAHHFS--DVRKAVREVARVLKQDGRFLL 113
>gb|AAD28459.1|AF127374_14 (AF127374) MitM [Streptomyces lavendulae]
Length = 283
Score = 68.7 bits (165), Expect = 6e-11
Identities = 44/125 (35%), Positives = 65/125 (51%), Gaps = 7/125 (5%)
Query: 41 KVLDLACGVGGFSFLLEDY-GFEVVGLDISEEMISKAKMYAKEK--SSNVEFIIGDAKKL 97
+VLDL CGVG + G V G+ +S E + +A A+E + F DA L
Sbjct: 74 RVLDLGCGVGTPGVRIARLSGAHVTGISVSHEQVVRANALAEEAGLADRARFQRADAMDL 133
Query: 98 PFEDNNFDYVIFIDSLVHFSPLELNQVFKEVKRVLKPTGKFIIYFTDMRELLPRLRESLV 157
PFED +FD VI ++S++H + QV +V RVL+P G+ ++ TD E P E
Sbjct: 134 PFEDESFDAVIALESIIHMP--DRAQVLAQVGRVLRPGGRLVL--TDFFERAPLAPEGRA 189
Query: 158 VGEKY 162
++Y
Sbjct: 190 AVQRY 194
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.141 0.408
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85009009
Number of Sequences: 2977
Number of extensions: 3548050
Number of successful extensions: 10904
Number of sequences better than 1.0e-10: 20
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 10867
Number of HSP's gapped (non-prelim): 20
length of query: 227
length of database: 189,106,746
effective HSP length: 54
effective length of query: 173
effective length of database: 156,788,448
effective search space: 27124401504
effective search space used: 27124401504
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 163 (67.9 bits)