BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2221 (speE) DE:spermidine synthase (speE)
(279 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75209 spermidine synthase (spee) PAB2221 - Pyrococcus abys... 570 e-162
sp|O57950|SPEE_PYRHO PROBABLE SPERMIDINE SYNTHASE (PUTRESCINE AM... 465 e-130
gi|11499915 spermidine synthase (speE) [Archaeoglobus fulgidus] ... 189 3e-47
sp|Q57761|SPEE_METJA PROBABLE SPERMIDINE SYNTHASE (PUTRESCINE AM... 184 9e-46
sp|Q9WZC2|SPEE_THEMA SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 183 2e-45
dbj|BAB07530.1| (AP001520) spermidine synthase [Bacillus halodur... 180 1e-44
sp|P70998|SPEE_BACSU SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 180 2e-44
sp|O66473|SPEE_AQUAE SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 179 4e-44
sp|Q9YE02|SPEE_AERPE PROBABLE SPERMIDINE SYNTHASE (PUTRESCINE AM... 176 2e-43
sp|Q09741|SPEE_SCHPO SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 176 2e-43
gb|AAF54417.1| (AE003683) CG8327 gene product [alt 1] [Drosophil... 176 2e-43
gb|AAC98040.1| (AC005990) Strong similarity to gb|AB006693 sperm... 172 4e-42
emb|CAB60361.1| (AL110485) contains similarity to Pfam domain: P... 167 1e-40
gi|6678131 spermidine synthase [Mus musculus] >gi|2498944|sp|Q64... 167 2e-40
sp|Q9X6R0|SPEE_PSEAE SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 165 6e-40
gb|AAD32692.1|AF143948_1 (AF143948) putative spermidine synthase... 165 6e-40
pir||T01492 spermidine synthase homolog F17O7.16 - Arabidopsis t... 165 6e-40
sp|O48661|SPEE_ARATH SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 165 6e-40
emb|CAB71155.1| (AJ271622) spermidine synthase [Plasmodium falci... 164 8e-40
sp|P09158|SPEE_ECOLI SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 164 1e-39
sp|O48658|SPE1_HYONI SPERMIDINE SYNTHASE 1 (PUTRESCINE AMINOPROP... 164 1e-39
emb|CAA07020.1| (AJ006414) spermidine synthase [Lycopersicon esc... 163 2e-39
gi|4507209 spermidine synthase; Spermidine synthase-1 [Homo sapi... 163 2e-39
sp|P19623|SPEE_HUMAN SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 162 3e-39
sp|O48660|SPEE_NICSY SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 162 4e-39
gb|AAD02232.1| (AF043109) spermidine synthase 2 [Pisum sativum] 162 5e-39
sp|Q9XY92|SPEE_DICDI SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 161 7e-39
sp|Q96556|SPE1_DATST SPERMIDINE SYNTHASE 1 (PUTRESCINE AMINOPROP... 161 1e-38
sp|O48659|SPE2_HYONI SPERMIDINE SYNTHASE 2 (PUTRESCINE AMINOPROP... 161 1e-38
sp|Q96557|SPE2_DATST SPERMIDINE SYNTHASE 2 (PUTRESCINE AMINOPROP... 160 2e-38
pir||B82842 spermidine synthase XF0143 [imported] - Xylella fast... 159 5e-38
dbj|BAA74542.1| (AB004322) putrescine N-methyltransferase [Nicot... 159 5e-38
gb|AAF14878.1|AF126809_1 (AF126809) putrescine N-methyltransfera... 159 5e-38
dbj|BAA74544.1| (AB004324) putrescine N-methyltransferase [Nicot... 158 6e-38
sp|O82147|SPEE_COFAR SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 158 8e-38
gb|AAF14880.1|AF126811_1 (AF126811) putrescine N-methyltransfera... 158 8e-38
gb|AAK41051.1| Spermidine synthase [Sulfolobus solfataricus] 157 1e-37
sp|Q9Y8H7|SPEE_NEUCR SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 157 1e-37
emb|CAB57546.1| (Y18930) putrescine aminopropyl transferase [Sul... 157 1e-37
gb|AAF14879.1|AF126810_1 (AF126810) putrescine N-methyltransfera... 157 1e-37
sp|Q42963|PNMT_TOBAC PUTRESCINE N-METHYLTRANSFERASE (PMT) (A411)... 157 1e-37
dbj|BAA82262.1| (AB018571) putrescine N-methyltransferase 2 [Atr... 156 2e-37
emb|CAC11499.1| (AL445064) spermidine synthase 2 related protein... 156 2e-37
gb|AAF14881.1|AF126812_1 (AF126812) putrescine N-methyltransfera... 156 3e-37
gb|AAB35050.1| spermidine synthase, putrescine aminopropyltransf... 155 4e-37
gb|AAD02231.1| (AF043108) spermidine synthase 1 [Pisum sativum] 155 5e-37
gb|AAF01311.1|AF184093_1 (AF184093) spermine synthase [Arabidops... 155 7e-37
gi|6325326 putrescine aminopropyltransferase (spermidine synthas... 154 1e-36
sp|P57305|SPEE_BUCAI SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYL... 154 1e-36
dbj|BAA82263.1| (AB018572) putrescine N-methyltransferase [Hyosc... 153 2e-36
dbj|BAB08415.1| (AB025622) spermidine synthase [Arabidopsis thal... 153 2e-36
dbj|BAA82261.1| (AB018570) putrescine N-methyltransferase 1 [Atr... 151 8e-36
gi|6323175 Spermine Synthase; Spe4p [Saccharomyces cerevisiae] >... 151 1e-35
emb|CAB61629.1| (AJ251298) spermidine synthase 1 [Oryza sativa] 150 2e-35
dbj|BAA74543.1| (AB004323) putrescine N-methyltransferase [Nicot... 143 2e-33
pir||D83048 hypothetical protein PA4774 [imported] - Pseudomonas... 139 4e-32
gb|AAG24612.1| (AF298195) spermidine synthase [Leishmania donovani] 128 7e-29
emb|CAB42358.1| (AL035213) spermidine synthase 1 [Leishmania major] 125 4e-28
pir||H70886 probable spermidine synthase - Mycobacterium tubercu... 100 2e-20
gb|AAC14108.1| (AF055854) spermidine synthase [Synechococcus PCC... 95 8e-19
pir||B70417 conserved hypothetical protein aq_1350 - Aquifex aeo... 91 2e-17
emb|CAB86120.1| (AL163003) putative spermidine synthase [Strepto... 85 1e-15
gb|AAF49935.1| (AE003542) CG4300 gene product [alt 2] [Drosophil... 83 5e-15
gb|AAF49934.1| (AE003542) CG4300 gene product [alt 1] [Drosophil... 83 5e-15
pir||T36545 probable spermidine synthase - Streptomyces coelicol... 77 2e-13
gi|4759152 spermine synthase; SpS [Homo sapiens] >gi|1082797|pir... 75 1e-12
sp|P52788|SPSY_HUMAN SPERMINE SYNTHASE (SPERMIDINE AMINOPROPYLTR... 75 1e-12
gb|AAD08634.1| (U53331) spermidine aminopropyltransferase [Homo ... 75 1e-12
gi|6678033 spermine synthase [Mus musculus] >gi|8134713|sp|P9735... 73 5e-12
emb|CAA08902.1| (AJ009864) spermine synthase [Danio rerio] >gi|4... 73 5e-12
emb|CAA08903.1| (AJ009865) spermine synthase [Fugu rubripes] 69 6e-11
>pir||B75209 spermidine synthase (spee) PAB2221 - Pyrococcus abyssi (strain
Orsay) >gi|5457630|emb|CAB49121.1| (AJ248283) spermidine
synthase (speE) [Pyrococcus abyssi]
Length = 279
Score = 570 bits (1453), Expect = e-162
Identities = 279/279 (100%), Positives = 279/279 (100%)
Query: 1 MEFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYH 60
MEFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYH
Sbjct: 1 MEFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYH 60
Query: 61 ESLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGID 120
ESLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGID
Sbjct: 61 ESLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGID 120
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFIKENSGFDVVIVDSTDPVGPAKMLFSREFYERAYE 180
GGLLDKMMKGECKRAKLIIGDGVEFIKENSGFDVVIVDSTDPVGPAKMLFSREFYERAYE
Sbjct: 121 GGLLDKMMKGECKRAKLIIGDGVEFIKENSGFDVVIVDSTDPVGPAKMLFSREFYERAYE 180
Query: 181 ALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASPWAFLVGIKGDL 240
ALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASPWAFLVGIKGDL
Sbjct: 181 ALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASPWAFLVGIKGDL 240
Query: 241 DFRKIDLERAKKLNLKYYDPENHETIFQMPRYVREMLKP 279
DFRKIDLERAKKLNLKYYDPENHETIFQMPRYVREMLKP
Sbjct: 241 DFRKIDLERAKKLNLKYYDPENHETIFQMPRYVREMLKP 279
>sp|O57950|SPEE_PYRHO PROBABLE SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE)
(SPDSY) >gi|7434037|pir||A71244 probable spermidine
synthase - Pyrococcus horikoshii
>gi|3256597|dbj|BAA29280.1| (AP000001) 280aa long
hypothetical spermidine synthase [Pyrococcus horikoshii]
Length = 280
Score = 465 bits (1183), Expect = e-130
Identities = 215/277 (77%), Positives = 249/277 (89%)
Query: 1 MEFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYH 60
MEFIEWYPRGYGVAFKVK KI E S++QKIEVYETEGFG+LLA+DGTVQLVTEGE+SYH
Sbjct: 4 MEFIEWYPRGYGVAFKVKRKILEEQSEYQKIEVYETEGFGKLLAIDGTVQLVTEGEKSYH 63
Query: 61 ESLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGID 120
E LVHP MLAHPNPRRVL+IGGGDGG IREVL+HEEVEE IMVEID++VIE++ K+IGID
Sbjct: 64 EPLVHPAMLAHPNPRRVLIIGGGDGGAIREVLKHEEVEEVIMVEIDKKVIEISAKYIGID 123
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFIKENSGFDVVIVDSTDPVGPAKMLFSREFYERAYE 180
GG+L+KM+ + ++ KLIIGDGV+FI+ENSGFDV+IVDSTDPVGPA+MLFS EFY+ AY
Sbjct: 124 GGILEKMLSDKHEKGKLIIGDGVKFIEENSGFDVIIVDSTDPVGPAEMLFSEEFYKNAYR 183
Query: 181 ALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASPWAFLVGIKGDL 240
ALNDPGIYVTQAGSVYLFTDE +TAY M +VFDKVY YSFPVIGYASPWAFLVG+KG +
Sbjct: 184 ALNDPGIYVTQAGSVYLFTDEFLTAYRKMRKVFDKVYYYSFPVIGYASPWAFLVGVKGSI 243
Query: 241 DFRKIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
DF K+D E+ KKL L+YYDP+ HET+FQMPRY+ +ML
Sbjct: 244 DFMKVDAEKGKKLGLEYYDPDKHETLFQMPRYIVQML 280
>gi|11499915 spermidine synthase (speE) [Archaeoglobus fulgidus]
>gi|6094333|sp|O27950|SPEE_ARCFU PROBABLE SPERMIDINE
SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|7434031|pir||F69541 spermidine synthase (speE)
homolog - Archaeoglobus fulgidus
>gi|2648179|gb|AAB88918.1| (AE000943) spermidine
synthase (speE) [Archaeoglobus fulgidus]
Length = 269
Score = 189 bits (476), Expect = 3e-47
Identities = 114/277 (41%), Positives = 170/277 (61%), Gaps = 21/277 (7%)
Query: 3 FIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHES 62
F E+Y G G+A KVK+K++E QK+E+YETE G++L +DG +QL E YHE
Sbjct: 4 FFEYYD-GCGLAIKVKKKLYEAEG-VQKVEIYETESMGKMLVIDGKIQLTELDEPFYHEM 61
Query: 63 LVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGG 122
LVH ML+H NPR+V VIGGGDGG +REVL+H VE A++V+ID VI+++ KF+ ID G
Sbjct: 62 LVHVPMLSHENPRKVAVIGGGDGGALREVLKH-NVERAVLVDIDRNVIDLSRKFLKIDHG 120
Query: 123 LLDKMMKGECKRAKLIIGDGVEFIKENSGFDVVIVDSTDPVGPAKMLFSREFYERAYEAL 182
E +R ++ I DG EF+++ FDV+IVDSTDPVG + LF REF+E A +
Sbjct: 121 AF------EDERVEIAIMDGKEFLRDCEIFDVIIVDSTDPVGVSDTLFDREFFELARQKC 174
Query: 183 NDPGIYVTQAGSVYLFTDELVTAYENMSRVFDK--VYAYSFPVIGYASPWAFLVGIKGDL 240
+ + Q+ S + + T N S F++ VY P A W+F++G G+
Sbjct: 175 D---VISLQSQSPLIQKEYFRTLLVN-SAPFERRDVYLSCVPTYPLAL-WSFIIG--GEY 227
Query: 241 DFRKIDLERAKKLNLK--YYDPENHETIFQMPRYVRE 275
DF ++ ER +++ K +Y+P+ H F +P ++++
Sbjct: 228 DFSNLE-ERFERIKGKTVHYNPDVHRAAFALPEWLKK 263
>sp|Q57761|SPEE_METJA PROBABLE SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE)
(SPDSY) >gi|2129288|pir||B64339 spermidine synthase (EC
2.5.1.16) - Methanococcus jannaschii
>gi|1591033|gb|AAB98300.1| (U67486) spermidine synthase
(speE) [Methanococcus jannaschii]
Length = 293
Score = 184 bits (463), Expect = 9e-46
Identities = 110/288 (38%), Positives = 168/288 (58%), Gaps = 23/288 (7%)
Query: 3 FIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHES 62
F E++ ++ +VK+ ++ S FQ+IE+ +T FG+ L LD T Q E YHE
Sbjct: 13 FTEYHNNNVALSVRVKDILYREKSGFQEIEIIDTYDFGKALILDNTFQTTERDEFIYHEL 72
Query: 63 LVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGG 122
+ H + HPNPR VLVIGGGDGGT+REV++H+ VE VE+DE+VIE K++
Sbjct: 73 ISHIPLFTHPNPRNVLVIGGGDGGTVREVVKHKSVETVDFVELDEKVIEACKKYMPKLSC 132
Query: 123 LLDKMMKGECKRAKLIIGDGVEFIKE-NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEA 181
+D ++ LII DG++++ E +DV+IVD DPVGPAK LF +EFY+ ++
Sbjct: 133 EIDN------EKVNLIITDGIKYVAETEKKYDVIIVDCPDPVGPAKGLFEKEFYKNVFKC 186
Query: 182 LNDPGIYVTQAGSVYLFTDELVTAYENMSRV-----FDKVYAYSFPVIGYASP-WAFLVG 235
LND GI V Q+ S L+ +L+ +N+ R F + Y++P+ Y S W+F +
Sbjct: 187 LNDDGIMVQQSESP-LYNLDLI---QNICRYLKDAGFKIIMPYTYPMPTYPSGFWSFTLA 242
Query: 236 IKG----DLDFRKIDLERAKKLNLKYYDPENHETIF-QMPRYVREMLK 278
K ++D +I E K + KYYD E H+ IF P+++++ +K
Sbjct: 243 SKKYNPLEVDEARIK-EALKDMETKYYDEEVHKGIFLAAPKFLKDAVK 289
>sp|Q9WZC2|SPEE_THEMA SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|7434038|pir||C72348 spermidine synthase - Thermotoga
maritima (strain MSB8)
>gi|4981177|gb|AAD35738.1|AE001739_1 (AE001739)
spermidine synthase [Thermotoga maritima]
Length = 296
Score = 183 bits (460), Expect = 2e-45
Identities = 102/281 (36%), Positives = 156/281 (55%), Gaps = 12/281 (4%)
Query: 3 FIEWYP-RGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
+ E+Y G+ K+ I+ G S Q+I+++E G + ALDG + E YHE
Sbjct: 19 YFEYYTGNNVGLFMKMNRVIYSGQSDIQRIDIFENPDLGVVFALDGITMTTEKDEFMYHE 78
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
L H M HPNP++VL+IGGGDGGT+REVL+H+ VE+AI+ E+D VIE A K++
Sbjct: 79 MLAHVPMFLHPNPKKVLIIGGGDGGTLREVLKHDSVEKAILCEVDGLVIEAARKYLKQTS 138
Query: 122 GLLDKMMKGECKRAKLIIGDGVEFIKE-NSGFDVVIVDSTDP-VGPAKMLFSREFYERAY 179
D RA+++I +G E++++ + FDV+I+DSTDP G LF+ EFY+ Y
Sbjct: 139 CGFDD------PRAEIVIANGAEYVRKFKNEFDVIIIDSTDPTAGQGGHLFTEEFYQACY 192
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYAS-PWAFLVGIKG 238
+AL + G++ + + AY +S+VF Y + Y S W++ KG
Sbjct: 193 DALKEDGVFSAETEDPFYDIGWFKLAYRRISKVFPITRVYLGFMTTYPSGMWSYTFASKG 252
Query: 239 DLDFRKIDLERAKKLN--LKYYDPENHETIFQMPRYVREML 277
+ D E+ +K N LKYY+ E H F +P +V++ L
Sbjct: 253 IDPIKDFDPEKVRKFNKELKYYNEEVHVASFALPNFVKKEL 293
>dbj|BAB07530.1| (AP001520) spermidine synthase [Bacillus halodurans]
Length = 275
Score = 180 bits (453), Expect = 1e-44
Identities = 99/271 (36%), Positives = 153/271 (55%), Gaps = 11/271 (4%)
Query: 11 YGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLA 70
+G+ K+K + + FQ+++V ETE FG +L LDG V + E YHE + H +
Sbjct: 13 FGITAKIKRTLHTEKTDFQQLDVIETEEFGNMLVLDGMVMTTEKDEFVYHEMVAHVPLFT 72
Query: 71 HPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMMK 129
HPNP+ VLV+GGGDGG IREVL+H VE+A +VEID +VIE + K++ I L D
Sbjct: 73 HPNPKHVLVVGGGDGGVIREVLKHPSVEKATLVEIDGKVIEYSKKYLPSIATALND---- 128
Query: 130 GECKRAKLIIGDG-VEFIKENSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGIY 188
R + + DG + K S +DV++VDST+PVGPA LF + FY+ +AL + GI+
Sbjct: 129 ---PRVDVQVDDGFLHIAKAESAYDVIMVDSTEPVGPAVKLFEKGFYQGIAKALKEDGIF 185
Query: 189 VTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASP-WAFLVGIKGDLDFRKIDL 247
V Q + + + + Y ++ +F Y+ + Y S W F +G K D ++
Sbjct: 186 VAQTDNPWFHGELIRKVYADVQEIFPITRLYTANIPTYPSGLWTFTIGSK-TYDPLHVEP 244
Query: 248 ERAKKLNLKYYDPENHETIFQMPRYVREMLK 278
R + KY+ + H+ F +PR+V +++K
Sbjct: 245 SRFYDIETKYFTKDLHKAAFALPRFVDDLIK 275
>sp|P70998|SPEE_BACSU SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|7434032|pir||G70057 spermidine synthase homolog ywhF
- Bacillus subtilis >gi|1565241|emb|CAB02516.1| (Z80360)
Unknown, highly similar to several spermidine synthases
[Bacillus subtilis] >gi|2636286|emb|CAB15777.1| (Z99123)
similar to spermidine synthase [Bacillus subtilis]
Length = 276
Score = 180 bits (452), Expect = 2e-44
Identities = 102/280 (36%), Positives = 158/280 (56%), Gaps = 15/280 (5%)
Query: 6 WYP----RGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
WY + +G+ KV + + ++FQ +E+ ETE FG +L LDG V + E YHE
Sbjct: 5 WYTEKQTKNFGITMKVNKTLHTEQTEFQHLEMVETEEFGNMLFLDGMVMTSEKDEFVYHE 64
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+ H + HPNP VLV+GGGDGG IRE+L+H V++A +V+ID +VIE + KF+
Sbjct: 65 MVAHVPLFTHPNPEHVLVVGGGDGGVIREILKHPSVKKATLVDIDGKVIEYSKKFLPSIA 124
Query: 122 GLLDKMMKGECKRAKLIIGDG-VEFIKENSGFDVVIVDSTDPVGPAKMLFSREFYERAYE 180
G LD R + + DG + K + +DV++VDST+PVGPA LF++ FY +
Sbjct: 125 GKLDD------PRVDVQVDDGFMHIAKSENQYDVIMVDSTEPVGPAVNLFTKGFYAGIAK 178
Query: 181 ALNDPGIYVTQAGSVYLFTDELVT-AYENMSRVFDKVYAYSFPVIGYASP-WAFLVGIKG 238
AL + GI+V Q + + FT EL+T ++ +F Y+ + Y S W F +G K
Sbjct: 179 ALKEDGIFVAQTDNPW-FTPELITNVQRDVKEIFPITKLYTANIPTYPSGLWTFTIGSK- 236
Query: 239 DLDFRKIDLERAKKLNLKYYDPENHETIFQMPRYVREMLK 278
D ++ R + KYY + H+ F +P++V +++K
Sbjct: 237 KYDPLAVEDSRFFDIETKYYTKDIHKAAFVLPKFVSDLIK 276
>sp|O66473|SPEE_AQUAE SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|7434033|pir||F70305 spermidine synthase - Aquifex
aeolicus >gi|2982813|gb|AAC06436.1| (AE000672)
spermidine synthase [Aquifex aeolicus]
Length = 280
Score = 179 bits (449), Expect = 4e-44
Identities = 102/256 (39%), Positives = 155/256 (59%), Gaps = 13/256 (5%)
Query: 15 FKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHPNP 74
+ V + +++G S +Q+I+V E+ FGR+L LDG VQL + E YHE L H + AHPNP
Sbjct: 19 YTVSKILYQGKSPYQEIQVIESPEFGRMLILDGVVQLDEKYEFLYHEYLAHVPLHAHPNP 78
Query: 75 RRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMMKGECK 133
R VL+IGGGDGGT+REVL+H+ VE A++V+ID+EVIEV+ K++ + G D
Sbjct: 79 RNVLIIGGGDGGTLREVLKHDIVERAVLVDIDKEVIEVSKKYLPTLSVGFQD-------P 131
Query: 134 RAKLIIGDGVEFIKE-NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGIYVTQA 192
RA ++ DG +++++ + FDV+IVDSTDPVG A +L + +F+ Y+AL + GI+V Q
Sbjct: 132 RAIVVNEDGYKYVQDYENEFDVIIVDSTDPVGFAHVLTTEDFFRHVYKALKEDGIFVAQT 191
Query: 193 GSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASP-WAFLVGIKGDLDFRKIDLERAK 251
S++ + + + + +VF V Y+ + YA W F + K + R
Sbjct: 192 ESIHYHLEMVSNIQQRLKKVFPIVDLYTSVIPIYAGYWWTFSIASK---KYPVRTPAREV 248
Query: 252 KLNLKYYDPENHETIF 267
K+ K YD + HE F
Sbjct: 249 KVQTKIYDADMHEYAF 264
>sp|Q9YE02|SPEE_AERPE PROBABLE SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE)
(SPDSY) >gi|7434035|pir||A72668 probable spermidine
synthase APE0767 - Aeropyrum pernix (strain K1)
>gi|5104430|dbj|BAA79745.1| (AP000060) 306aa long
hypothetical spermidine synthase [Aeropyrum pernix]
Length = 306
Score = 176 bits (443), Expect = 2e-43
Identities = 104/289 (35%), Positives = 160/289 (54%), Gaps = 21/289 (7%)
Query: 3 FIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHES 62
F+EW +++ IF+G S+FQ+I V G G++L LDG Q E YHE+
Sbjct: 8 FLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQSSESDEFMYHEA 67
Query: 63 LVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGG 122
LVHP M+ H +PR+VL++GGG+G T+REVL+H VE+A+MV+IDE V+ VA +
Sbjct: 68 LVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVVNVAREH------ 121
Query: 123 LLDKMMKG--ECKRAKLIIGDGVEFI--KENSGFDVVIVDSTDPV--GPAKMLFSREFYE 176
L + +G + RA+++I D ++ K +GFDVVI D DP+ GPA L+S E+Y
Sbjct: 122 -LREWHRGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYSEEYYR 180
Query: 177 RAYEALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASPWAFLVG- 235
+ +N G++VTQA S+ T+ ++RVF V +Y V + S W F+V
Sbjct: 181 MVKDVMNPGGVFVTQAVSISHLTEYHAIIRNTVARVFKHVESYGVYVPSFDSMWGFVVAS 240
Query: 236 ------IKGDLDFRKIDL-ERAKKLNLKYYDPENHETIFQMPRYVREML 277
I GD F + L + + L++ D + + +P+ RE +
Sbjct: 241 DDKDPRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAI 289
>sp|Q09741|SPEE_SCHPO SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|7493422|pir||T39374 spermidine synthase - fission
yeast (Schizosaccharomyces pombe)
>gi|984695|emb|CAA90820.1| (Z54140) spermidine synthase
[Schizosaccharomyces pombe]
Length = 298
Score = 176 bits (442), Expect = 2e-43
Identities = 104/276 (37%), Positives = 161/276 (57%), Gaps = 16/276 (5%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KVK+ ++ G SK+Q + V+E+E +G +L LDG +Q E SY E + H +
Sbjct: 25 GQAMTLKVKKVLYAGKSKYQDVLVFESETYGHVLVLDGAIQATERDEFSYQEMIAHLALN 84
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+HPNP++VLVIGGGDGG +REV++HE VEEAI+ +IDE+VI+V+ ++ L +M
Sbjct: 85 SHPNPKKVLVIGGGDGGVLREVVKHECVEEAILCDIDEDVIKVSKQY-------LPEMSA 137
Query: 130 G-ECKRAKLIIGDGVEFIKE-NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
G + K+ IGDG +F+++ + FDV+I DS+DP GPA+ LF + +++ +AL G+
Sbjct: 138 GFNHPKVKVHIGDGFKFLQDYQNTFDVIITDSSDPDGPAEALFQKPYFQLLSDALRGGGV 197
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKV-YAYSFPVIGYASPWAFLVGIKG---DLD-- 241
TQA +++ + + VF V YAY+ + F+V K DL
Sbjct: 198 ITTQAECMWIHLGVISNVLTAVKTVFPNVRYAYTTIPTYPSGSIGFVVASKDASIDLSKP 257
Query: 242 FRKIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
RK E KL KYY+ E H F +P + R+++
Sbjct: 258 LRKWSPEEENKL-CKYYNSEIHAASFVLPTFARDVV 292
>gb|AAF54417.1| (AE003683) CG8327 gene product [alt 1] [Drosophila melanogaster]
Length = 287
Score = 176 bits (442), Expect = 2e-43
Identities = 104/272 (38%), Positives = 152/272 (55%), Gaps = 13/272 (4%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KVKE I + S+FQ I++ ETE +GR L LDG +Q E SY E + +
Sbjct: 19 GQSFSLKVKEVIHKEKSRFQDIQIVETETYGRCLILDGIIQCTARDEFSYQEMISFLPLC 78
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
AHPNP++VL++GGGDGG REV++H VEE VEID+ V+E++ ++ L M
Sbjct: 79 AHPNPKKVLIVGGGDGGVAREVVKHPLVEEVHQVEIDDRVVELSKQY-------LPAMAC 131
Query: 130 GEC-KRAKLIIGDGVEFIKENSG-FDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
G ++ KL IGDG +++K++ FDV+I DS+DP+GPA LF +YE AL D GI
Sbjct: 132 GFANEKLKLTIGDGFDYMKKHKNEFDVIITDSSDPIGPAVSLFQESYYELMKHALKDDGI 191
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKV-YAYSFPVIGYASPWAFLVG-IKGDLDFR-- 243
+Q GS +L D + F KV YA + F++G + + DF
Sbjct: 192 VCSQGGSFWLDLDYIKKTMSGCKEHFAKVAYAVTSVPSYPCGHIGFVMGSLNKNQDFATP 251
Query: 244 KIDLERAKKLNLKYYDPENHETIFQMPRYVRE 275
K+ ++L+YY E H F +PR+V++
Sbjct: 252 KLGKSEIDSIDLRYYSSEVHSAAFALPRWVQK 283
>gb|AAC98040.1| (AC005990) Strong similarity to gb|AB006693 spermidine synthase
from Arabidopsis thaliana. ESTs gb|AA389822, gb|T41794,
gb|N38455, gb|AI100106, gb|F14442 and gb|F14256 come
from this gene. >gi|6468489|emb|CAB61614.1| (AJ251296)
spermidine synthase 1 [Arabidopsis thaliana]
>gi|6682289|emb|CAB64644.1| (AJ252215) spermidine
synthase [Arabidopsis thaliana]
Length = 334
Score = 172 bits (432), Expect = 4e-42
Identities = 104/286 (36%), Positives = 160/286 (55%), Gaps = 29/286 (10%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 57 GEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLC 116
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ PNP++VLVIGGGDGG +REV RH +E+ M EID+ V++V+ +F D +
Sbjct: 117 SIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFP------DVAIG 170
Query: 130 GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R L+IGDGV F+K +D VIVDS+DP+GPAK LF + F++ AL G+
Sbjct: 171 YEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPIGPAKELFEKPFFQSVARALRPGGV 230
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWAFLVGIKG- 238
TQA S++L D + N +F V Y VIG+ L +G
Sbjct: 231 VCTQAESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGF-----MLCSTEGP 285
Query: 239 DLDFR----KIDLERAKKLN--LKYYDPENHETIFQMPRYVREMLK 278
D+DF+ ID E + K N LK+Y+ E H F +P + +++++
Sbjct: 286 DVDFKHPLNPID-ESSSKSNGPLKFYNAEIHSAAFCLPSFAKKVIE 330
>emb|CAB60361.1| (AL110485) contains similarity to Pfam domain: PF01564
(Spermine/spermidine synthase), Score=436.7,
E-value=6.5e-128, N=1~cDNA EST yk489d8.3 comes from this
gene~cDNA EST yk198a10.5 comes from this gene~cDNA EST
yk427b4.5 comes from this gene~cDNA EST >
Length = 366
Score = 167 bits (419), Expect = 1e-40
Identities = 111/329 (33%), Positives = 161/329 (48%), Gaps = 67/329 (20%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + +VK+ +F SK+Q + V+E+ +G +L LDG VQ E SY E L H M
Sbjct: 45 GQAFSLQVKKVLFHEKSKYQDVLVFESTTYGNVLVLDGIVQATERDEFSYQEMLAHLPMF 104
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMM 128
AHP+P+RVL+IGGGDGG +REVL+HE VE+ M EIDE VI+VA KF+ G+ G
Sbjct: 105 AHPDPKRVLIIGGGDGGILREVLKHESVEKVTMCEIDEMVIDVAKKFLPGMSCGF----- 159
Query: 129 KGECKRAKLIIGDGVEFIKENSG-FDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
+ L GDG EF+K + FDV+I DS+DPVGPA+ LF + +YE +AL + GI
Sbjct: 160 --SHPKLDLFCGDGFEFLKNHKNEFDVIITDSSDPVGPAESLFGQSYYELLRDALKEDGI 217
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVF-------DKVYAYSFPVIGY-----------ASP 229
+Q +L + T + +F V Y+ ++GY S
Sbjct: 218 LSSQGECPWLDMKLISTVIHSARDLFPVAKYAVGSVPTYTSGLMGYILCAKDKVNTGESV 277
Query: 230 WAFLVGI-------------------------KGDLDF---------------RKIDLER 249
W L I G + + R + E+
Sbjct: 278 WLHLPLIAHLVAFNRKIFPAVTYAQSIVSTYPSGSMGYLICAKNANRDVTTPARTLTAEQ 337
Query: 250 AKKLNLKYYDPENHETIFQMPRYVREMLK 278
K LNL++Y+ E H+ F +P++V+ L+
Sbjct: 338 IKALNLRFYNSEVHKAAFVLPQFVKNALE 366
>gi|6678131 spermidine synthase [Mus musculus] >gi|2498944|sp|Q64674|SPEE_MOUSE
SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE)
(SPDSY) >gi|309502|gb|AAC37666.1| (L19311) spermidine
synthase [Mus musculus] >gi|1061192|emb|CAA91561.1|
(Z67748) spermidine synthase [Mus musculus]
>gi|1097138|prf||2113276A spermidine synthase [Mus
musculus]
Length = 302
Score = 167 bits (418), Expect = 2e-40
Identities = 96/276 (34%), Positives = 150/276 (53%), Gaps = 15/276 (5%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G ++ +V++ + S++Q I V+ ++ +G +L LDG +Q E SY E + + +
Sbjct: 30 GQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTERDEFSYQEMIANLPLC 89
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMM 128
+HPNPR+VL+IGGGDGG +REV++H VE + EIDE+VIEV+ KF+ G+ G
Sbjct: 90 SHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIEVSKKFLPGMAVGF----- 144
Query: 129 KGECKRAKLIIGDGVEFIKEN-SGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
+ L +GDG EF+K+N FDV+I DS+DP+GPA+ LF +Y+ AL + GI
Sbjct: 145 --SSSKLTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGI 202
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKV-YAY-SFPVIGYASPWAFLVGIKGDLDFR-- 243
Q +L D + +F V YAY S P L +FR
Sbjct: 203 LCCQGECQWLHLDLIKEMRHFCKSLFPVVDYAYCSIPTYPSGQIGFMLCSKNPSTNFREP 262
Query: 244 --KIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
++ + +++ LKYY+ + H F +P + R+ L
Sbjct: 263 VQQLTQAQVEQMQLKYYNSDMHRAAFVLPEFTRKAL 298
>sp|Q9X6R0|SPEE_PSEAE SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|11352590|pir||G83433 spermidine synthase PA1687
[imported] - Pseudomonas aeruginosa (strain PAO1)
>gi|9947660|gb|AAG05076.1|AE004596_2 (AE004596)
spermidine synthase [Pseudomonas aeruginosa]
Length = 286
Score = 165 bits (413), Expect = 6e-40
Identities = 101/287 (35%), Positives = 153/287 (53%), Gaps = 20/287 (6%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
++ E +GYG F + + E ++ Q + ++E GR++ALDG +Q E YHE
Sbjct: 3 DYQETLYQGYGQRFSIDNMLHEVRTEHQHLVIFENARMGRVMALDGVIQTTEADEFIYHE 62
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GID 120
L H +LAH RRVL+IGGGDGG +REV +H+ VE MVEID V+++ +F+
Sbjct: 63 MLTHVPILAHGAARRVLIIGGGDGGMLREVAKHKSVERITMVEIDGTVVDMCKEFLPNHS 122
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFI-KENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
G D R L+I DG+ F+ FDV+I DSTDP+GP ++LFS FY+
Sbjct: 123 QGAFDD------PRLNLVIDDGMRFVATTEERFDVIISDSTDPIGPGEVLFSENFYQACR 176
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPV---IGYASPWAFLVGI 236
LN+ GI VTQ G+ ++ +E+ T +F + Y V IG A +A+
Sbjct: 177 RCLNEGGILVTQNGTPFMQLEEVRTTAARTDGLFADWHFYQAAVPTYIGGAMTFAWGSTH 236
Query: 237 KGDLDFRKIDLE------RAKKLNLKYYDPENHETIFQMPRYVREML 277
+G R++ LE R + +YY+ + H F +P+YV + +
Sbjct: 237 EG---LRRLPLETLRQRFRDSGIATRYYNADIHLGAFALPQYVLQAI 280
>gb|AAD32692.1|AF143948_1 (AF143948) putative spermidine synthase [Pseudomonas aeruginosa]
Length = 336
Score = 165 bits (413), Expect = 6e-40
Identities = 101/287 (35%), Positives = 153/287 (53%), Gaps = 20/287 (6%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
++ E +GYG F + + E ++ Q + ++E GR++ALDG +Q E YHE
Sbjct: 53 DYQETLYQGYGQRFSIDNMLHEVRTEHQHLVIFENARMGRVMALDGVIQTTEADEFIYHE 112
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GID 120
L H +LAH RRVL+IGGGDGG +REV +H+ VE MVEID V+++ +F+
Sbjct: 113 MLTHVPILAHGAARRVLIIGGGDGGMLREVAKHKSVERITMVEIDGTVVDMCKEFLPNHS 172
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFI-KENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
G D R L+I DG+ F+ FDV+I DSTDP+GP ++LFS FY+
Sbjct: 173 QGAFDD------PRLNLVIDDGMRFVATTEERFDVIISDSTDPIGPGEVLFSENFYQACR 226
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPV---IGYASPWAFLVGI 236
LN+ GI VTQ G+ ++ +E+ T +F + Y V IG A +A+
Sbjct: 227 RCLNEGGILVTQNGTPFMQLEEVRTTAARTDGLFADWHFYQAAVPTYIGGAMTFAWGSTH 286
Query: 237 KGDLDFRKIDLE------RAKKLNLKYYDPENHETIFQMPRYVREML 277
+G R++ LE R + +YY+ + H F +P+YV + +
Sbjct: 287 EG---LRRLPLETLRQRFRDSGIATRYYNADIHLGAFALPQYVLQAI 330
>pir||T01492 spermidine synthase homolog F17O7.16 - Arabidopsis thaliana
>gi|3176685|gb|AAC18808.1| (AC003671) Strong similarity
to spermidine synthase 1, gb|Y08252 and possibly closer
similarity to spermidine synthase 2 gb|Y08253 from
Datura stramonium. ESTs gb|N38155, gb|T41738,
gb|AA597626, gb|AA712967 and gb|AA712346 come from this
gene. [Arabid> >gi|6468491|emb|CAB61615.1| (AJ251297)
spermidine synthase 2 [Arabidopsis thaliana]
Length = 340
Score = 165 bits (413), Expect = 6e-40
Identities = 102/286 (35%), Positives = 156/286 (53%), Gaps = 29/286 (10%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 61 GEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLC 120
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ NP++VLVIGGGDGG +REV RH VE+ + EID+ V++VA ++ + +
Sbjct: 121 SISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFP------NVAVG 174
Query: 130 GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R LIIGDGV F+K +D VIVDS+DP+GPAK LF + F+E AL G+
Sbjct: 175 YEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPIGPAKELFEKPFFESVNRALRPGGV 234
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWAFLVGIKG- 238
TQA S++L D + N +F V Y VIG+ L +G
Sbjct: 235 VCTQAESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGF-----MLCSSEGP 289
Query: 239 DLDFRK----IDLERA---KKLNLKYYDPENHETIFQMPRYVREML 277
+DF+K ID + + LKYY+ E H F +P + ++++
Sbjct: 290 QVDFKKPVSLIDTDESSIKSHCPLKYYNAEIHSAAFCLPSFAKKVI 335
>sp|O48661|SPEE_ARATH SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|2821961|dbj|BAA24536.1| (AB006693) spermidine
synthase [Arabidopsis thaliana]
Length = 293
Score = 165 bits (413), Expect = 6e-40
Identities = 102/286 (35%), Positives = 156/286 (53%), Gaps = 29/286 (10%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 14 GEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLC 73
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ NP++VLVIGGGDGG +REV RH VE+ + EID+ V++VA ++ + +
Sbjct: 74 SISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFP------NVAVG 127
Query: 130 GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R LIIGDGV F+K +D VIVDS+DP+GPAK LF + F+E AL G+
Sbjct: 128 YEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPIGPAKELFEKPFFESVNRALRPGGV 187
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWAFLVGIKG- 238
TQA S++L D + N +F V Y VIG+ L +G
Sbjct: 188 VCTQAESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGF-----MLCSSEGP 242
Query: 239 DLDFRK----IDLERA---KKLNLKYYDPENHETIFQMPRYVREML 277
+DF+K ID + + LKYY+ E H F +P + ++++
Sbjct: 243 QVDFKKPVSLIDTDESSIKSHCPLKYYNAEIHSAAFCLPSFAKKVI 288
>emb|CAB71155.1| (AJ271622) spermidine synthase [Plasmodium falciparum]
Length = 321
Score = 164 bits (412), Expect = 8e-40
Identities = 99/277 (35%), Positives = 154/277 (54%), Gaps = 13/277 (4%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + ++K+ ++E SK+Q + V+E+ +G++L LDG +QL + E +YHE
Sbjct: 46 EFSIMWP-GQAFSVEIKKILYETKSKYQNVLVFESTTYGKVLVLDGVIQLTEKDEFAYHE 104
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVA-IKFIGID 120
+ H M P+ VLV+GGGDGG IRE+ +++ VE + EIDE VIEV+ I F I
Sbjct: 105 MMTHIPMTVSKEPKNVLVVGGGDGGIIRELCKYKSVENIDICEIDETVIEVSKIYFKNIS 164
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFIKE-NSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
G D KR + I D +F++ + +DV+IVDS+DP+GPA+ LF++ FYE+ Y
Sbjct: 165 CGYED-------KRVNVFIEDASKFLENVTNTYDVIIVDSSDPIGPAETLFNQNFYEKIY 217
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGY-ASPWAFLVGIKG 238
AL G V Q S+++ + ++F KV + + Y L K
Sbjct: 218 NALKPNGYCVAQCESLWIHVGTIKNMIGYAKKLFKKVEYANISIPTYPCGCIGILCCSKT 277
Query: 239 DLDFRKID--LERAKKLNLKYYDPENHETIFQMPRYV 273
D K + LE + +LKYY+ ENH F++P ++
Sbjct: 278 DTGLTKPNKKLESKEFADLKYYNYENHSAAFKLPAFL 314
>sp|P09158|SPEE_ECOLI SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|66609|pir||SYECSD spermidine synthase (EC 2.5.1.16)
- Escherichia coli >gi|147852|gb|AAA24643.1| (J02804)
spermidine synthase [Escherichia coli]
>gi|473788|dbj|BAA05577.1| (D26562) 'spermidine
synthase' [Escherichia coli] >gi|1786312|gb|AAC73232.1|
(AE000121) spermidine synthase = putrescine
aminopropyltransferase [Escherichia coli K12]
Length = 288
Score = 164 bits (411), Expect = 1e-39
Identities = 95/275 (34%), Positives = 140/275 (50%), Gaps = 14/275 (5%)
Query: 11 YGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLA 70
+G F V ++ + Q + ++E FGR++ALDG VQ E YHE + H +LA
Sbjct: 15 FGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLA 74
Query: 71 HPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMMK 129
H + + VL+IGGGDG +REV RH+ VE MVEID V+ +++ + G D
Sbjct: 75 HGHAKHVLIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQYLPNHNAGSYDD--- 131
Query: 130 GECKRAKLIIGDGVEFIKENS-GFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGIY 188
R KL+I DGV F+ + S FDV+I D TDP+GP + LF+ FYE LN GI+
Sbjct: 132 ---PRFKLVIDDGVNFVNQTSQTFDVIISDCTDPIGPGESLFTSAFYEGCKRCLNPGGIF 188
Query: 189 VTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASPWAFLVGIKGDLDFRKIDLE 248
V Q G +L +E + ++ +S F V Y + Y + R + E
Sbjct: 189 VAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDNDALRHLSTE 248
Query: 249 ------RAKKLNLKYYDPENHETIFQMPRYVREML 277
A L +YY+P H F +P+Y+++ L
Sbjct: 249 IIQARFLASGLKCRYYNPAIHTAAFALPQYLQDAL 283
>sp|O48658|SPE1_HYONI SPERMIDINE SYNTHASE 1 (PUTRESCINE AMINOPROPYLTRANSFERASE 1) (SPDSY
1) >gi|2821955|dbj|BAA24533.1| (AB006690) spermidine
synthase 1 [Hyoscyamus niger]
Length = 315
Score = 164 bits (410), Expect = 1e-39
Identities = 101/281 (35%), Positives = 156/281 (54%), Gaps = 18/281 (6%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 37 GEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLC 96
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ PNP++VLVIGGGDGG +REV RH VE+ + EID+ V+EVA +F D +
Sbjct: 97 SIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVVEVAKEFFP------DVAVG 150
Query: 130 GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R L IGDGV F+K +D VIVDS+DP+GPA+ LF + F+E AL G+
Sbjct: 151 YEDPRVNLHIGDGVAFLKNVPAGTYDAVIVDSSDPIGPAQELFEKPFFESIARALRPGGV 210
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI-GYASPWA--FLVGIKGD-LDFR 243
TQA S++L + N ++F Y++ + Y S L +G +DF+
Sbjct: 211 VSTQAESIWLHMHIIEEIVANCRQIFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPAVDFK 270
Query: 244 K----IDLERAKK--LNLKYYDPENHETIFQMPRYVREMLK 278
ID E K LK+Y+ E H+ F +P + + +++
Sbjct: 271 NPINPIDDESGPKTIAPLKFYNSEIHQASFCLPSFAKRVIE 311
>emb|CAA07020.1| (AJ006414) spermidine synthase [Lycopersicon esculentum]
Length = 342
Score = 163 bits (409), Expect = 2e-39
Identities = 102/281 (36%), Positives = 159/281 (56%), Gaps = 18/281 (6%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 64 GEAHSLKVEKILFQGKSDYQNVLVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLC 123
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ PNP++VLVIGGGDGG +REV RH VE+ + EID+ V+EVA +F D +
Sbjct: 124 SIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVVEVAKQFFP------DVAVG 177
Query: 130 GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R L IGDGV F+K +D VIVDS+DP+GPA+ LF + F+E +AL G+
Sbjct: 178 YEDPRVNLHIGDGVAFLKNVPAGTYDAVIVDSSDPIGPAQELFEKPFFESIAKALRPGGV 237
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI-GYASPWA--FLVGIKGD-LDFR 243
TQA S++L + N ++F Y++ + Y S L +G +DF+
Sbjct: 238 VSTQAESIWLHMHIIEEIVANCRQIFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPAVDFK 297
Query: 244 K----IDLER-AKKLN-LKYYDPENHETIFQMPRYVREMLK 278
ID E AK + LK+Y+ E H+ F +P + + +++
Sbjct: 298 NPINPIDDESPAKSIEPLKFYNSEIHQASFCLPSFAKRVIE 338
>gi|4507209 spermidine synthase; Spermidine synthase-1 [Homo sapiens]
>gi|531202|gb|AAA36633.1| (M34338) spermidine synthase
[Homo sapiens]
Length = 302
Score = 163 bits (408), Expect = 2e-39
Identities = 92/276 (33%), Positives = 151/276 (54%), Gaps = 15/276 (5%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G ++ +V++ + S++Q I V+ ++ +G +L LDG +Q E SY E + + +
Sbjct: 30 GQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTERDEFSYQEMIANLPLC 89
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMM 128
+HPNPR+VL+IGGGDGG +REV++H VE + EIDE+VI+V+ KF+ G+ G
Sbjct: 90 SHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIQVSKKFLPGMAIGY----- 144
Query: 129 KGECKRAKLIIGDGVEFIKEN-SGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
+ L +GDG EF+K+N FDV+I DS+DP+GPA+ LF +Y+ AL + G+
Sbjct: 145 --SSSKVTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGV 202
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKV-YAY-SFPVIGYASPWAFLVGIKGDLDFRK- 244
Q +L D + + +F V YAY + P L +F++
Sbjct: 203 LCCQGECEWLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEP 262
Query: 245 ---IDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
+ ++ ++ LKYY+ + H F +P + R+ L
Sbjct: 263 VQPLTQQQVAQMQLKYYNSDVHRAAFVLPEFARKAL 298
>sp|P19623|SPEE_HUMAN SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|88626|pir||A32610 spermidine synthase (EC 2.5.1.16)
- human >gi|338394|gb|AAA60574.1| (M64231) spermidine
synthase [Homo sapiens]
Length = 302
Score = 162 bits (407), Expect = 3e-39
Identities = 92/276 (33%), Positives = 151/276 (54%), Gaps = 15/276 (5%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G ++ +V++ + S++Q I V+ ++ +G +L LDG +Q E SY E + + +
Sbjct: 30 GQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTERDEFSYQEMIANLPLC 89
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMM 128
+HPNPR+VL+IGGGDGG +REV++H VE + EIDE+VI+V+ KF+ G+ G
Sbjct: 90 SHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIQVSKKFLPGMAIGY----- 144
Query: 129 KGECKRAKLIIGDGVEFIKEN-SGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
+ L +GDG EF+K+N FDV+I DS+DP+GPA+ LF +Y+ AL + G+
Sbjct: 145 --SSSKLTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGV 202
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKV-YAY-SFPVIGYASPWAFLVGIKGDLDFRK- 244
Q +L D + + +F V YAY + P L +F++
Sbjct: 203 LCCQGECQWLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEP 262
Query: 245 ---IDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
+ ++ ++ LKYY+ + H F +P + R+ L
Sbjct: 263 VQPLTQQQVAQMQLKYYNSDVHRAAFVLPEFARKAL 298
>sp|O48660|SPEE_NICSY SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE)
(AMINOPROPYLTRANSFERASE) >gi|7434034|pir||T15045
spermidine synthase (EC 2.5.1.16) - wood tobacco
>gi|2821959|dbj|BAA24535.1| (AB006692) spermidine
synthase [Nicotiana sylvestris]
Length = 314
Score = 162 bits (406), Expect = 4e-39
Identities = 98/282 (34%), Positives = 154/282 (53%), Gaps = 19/282 (6%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 35 GEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMIAHLPLC 94
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ PNP++VLVIGGGDGG +REV RH VE+ + EID+ V+EV+ +F D +
Sbjct: 95 SIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVVEVSKQFFP------DVAVG 148
Query: 130 GECKRAKLIIGDGVEFIKENSG--FDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R L IGDGV F+K + +D VIVDS+DP+GPA+ LF + F+E AL G+
Sbjct: 149 YEDPRVNLHIGDGVAFLKNVAAGTYDAVIVDSSDPIGPAQELFEKPFFESIARALRPGGV 208
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGY--ASPWAFLVGIK 237
TQA S++L + N ++F V Y +IG+ S V K
Sbjct: 209 VSTQAESIWLHMHIIEEIVANCRQIFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPAVDFK 268
Query: 238 GDLDFRKIDLERAKKLN-LKYYDPENHETIFQMPRYVREMLK 278
++ D K L +K+Y+ E H+ F +P + + +++
Sbjct: 269 NPINPIDDDASHNKTLGPMKFYNSELHKASFCLPSFAKRVIE 310
>gb|AAD02232.1| (AF043109) spermidine synthase 2 [Pisum sativum]
Length = 342
Score = 162 bits (405), Expect = 5e-39
Identities = 99/281 (35%), Positives = 155/281 (54%), Gaps = 20/281 (7%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + K+++ +F+G S++QK+ V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 64 GEAHSLKIEKILFQGKSEYQKVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLC 123
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMM 128
+ PNP++VLVIGGGDGG +REV RH +E+ + EID V+EV+ +F + G D
Sbjct: 124 SIPNPKKVLVIGGGDGGVLREVARHSSIEKIDICEIDNMVVEVSKQFFPEVAVGFND--- 180
Query: 129 KGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPG 186
R L IGDGV F+K +D VIVDS+DP+GPA+ LF + F++ AL G
Sbjct: 181 ----PRVTLRIGDGVAFLKAAPEGTYDAVIVDSSDPIGPAQELFEKPFFQSVARALRPGG 236
Query: 187 IYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI-GYASPWA--FLVGIKGDL-DF 242
+ TQA S++L D + N +F Y++ + Y S L +G L DF
Sbjct: 237 VMCTQAESIWLHMDIIEDIVSNCRHIFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPLVDF 296
Query: 243 R----KIDLERAKK--LNLKYYDPENHETIFQMPRYVREML 277
+ ID + +K LK+Y+ E H F +P + + +
Sbjct: 297 KHPVNPIDQKDCQKSVRPLKFYNSEIHTAAFCLPSFAKRKI 337
>sp|Q9XY92|SPEE_DICDI SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|4903273|gb|AAD32851.1|AF069757_1 (AF069757)
spermidine synthase [Dictyostelium discoideum]
Length = 284
Score = 161 bits (404), Expect = 7e-39
Identities = 97/280 (34%), Positives = 154/280 (54%), Gaps = 12/280 (4%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
E E++P G + +V++ + S++Q I V++++ FG +L LDG +Q E +Y E
Sbjct: 11 EISEFWP-GNSFSLEVEKVLHHEKSEYQDILVFKSKSFGNVLVLDGVIQATERDEFAYQE 69
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+ H + +HP+P+RVLV+GGGDGG +REV++H VE + EID+ VIE + F
Sbjct: 70 MITHIPLFSHPSPKRVLVVGGGDGGVLREVVKHPLVESVTLCEIDKGVIEASRNF----- 124
Query: 122 GLLDKMMKG-ECKRAKLIIGDGVEFIKENSG-FDVVIVDSTDPVGPAKMLFSREFYERAY 179
L M G + + L IGDG+EF+++ G FDV+I DS+DP+GPA+ LF R +YE
Sbjct: 125 --LPNMRVGFDHPKVTLFIGDGMEFMRQRKGEFDVIITDSSDPIGPAQGLFERAYYELLK 182
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKV-YAY-SFPVIGYASPWAFLVGIK 237
AL GI +Q S++L D + ++ V YAY S P S L +
Sbjct: 183 AALAPGGIVCSQCESMWLHLDTIKGLTTFCKELYPNVEYAYTSIPSYPGGSIGFILCSLG 242
Query: 238 GDLDFRKIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
G ++ + ++YY+ E H+ F +P++ + L
Sbjct: 243 GSTKAPIREITPEVQSQMQYYNGEVHKASFVLPQFAAKKL 282
>sp|Q96556|SPE1_DATST SPERMIDINE SYNTHASE 1 (PUTRESCINE AMINOPROPYLTRANSFERASE 1) (SPDSY
1) >gi|1561577|emb|CAA69420.1| (Y08252) spermidine
synthase 1 [Datura stramonium]
Length = 308
Score = 161 bits (402), Expect = 1e-38
Identities = 98/282 (34%), Positives = 152/282 (53%), Gaps = 19/282 (6%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 29 GEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLC 88
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ PNP++VLVIGGGDGG +REV RH VE+ + EID+ VI+V+ +F + +
Sbjct: 89 SIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVIDVSKQFFP------NVAIG 142
Query: 130 GECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R KL +GDGV F+K +D VIVDS+DP+GPA+ LF + F+E AL G+
Sbjct: 143 YEDPRVKLHVGDGVAFLKFVAEGTYDAVIVDSSDPIGPAQELFEKPFFESVARALRPGGV 202
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGY--ASPWAFLVGIK 237
TQA S++L + N ++F V Y VIG+ S V K
Sbjct: 203 VCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFK 262
Query: 238 GDLDFRKIDLERAKKLN-LKYYDPENHETIFQMPRYVREMLK 278
++ D K LK+Y+ E H F +P + + +++
Sbjct: 263 NPINPVDADDSHTKTRGPLKFYNSEIHSASFCLPSFAKRVIE 304
>sp|O48659|SPE2_HYONI SPERMIDINE SYNTHASE 2 (PUTRESCINE AMINOPROPYLTRANSFERASE 2) (SPDSY
2) >gi|2821957|dbj|BAA24534.1| (AB006691) spermidine
synthase 2 [Hyoscyamus niger]
Length = 308
Score = 161 bits (402), Expect = 1e-38
Identities = 98/282 (34%), Positives = 152/282 (53%), Gaps = 19/282 (6%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 29 GEAHSLKVEKILFQGKSDYQNVVVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLC 88
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ PNP++VLVIGGGDGG +REV RH VE+ + EID+ VI+V+ +F + +
Sbjct: 89 SIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVIDVSKQFFP------NVAIG 142
Query: 130 GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R KL +GDGV F+K +D VIVDS+DP+GPA+ LF + F+E AL G+
Sbjct: 143 YEDPRVKLHVGDGVAFLKNVPEGTYDAVIVDSSDPIGPAQELFEKPFFESVARALCPGGV 202
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGY--ASPWAFLVGIK 237
TQA S++L + N ++F V Y VIG+ S V K
Sbjct: 203 VCTQAESIWLHMHIIEDIVSNCRQIFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFK 262
Query: 238 GDLDFRKIDLERAKKLN-LKYYDPENHETIFQMPRYVREMLK 278
++ D K LK+Y+ E H F +P + + +++
Sbjct: 263 NPINPIDADDSHTKTRGPLKFYNSEIHSASFCLPSFAKRVIE 304
>sp|Q96557|SPE2_DATST SPERMIDINE SYNTHASE 2 (PUTRESCINE AMINOPROPYLTRANSFERASE 2) (SPDSY
2) >gi|1561579|emb|CAA69421.1| (Y08253) spermidine
synthase 2 [Datura stramonium]
Length = 317
Score = 160 bits (400), Expect = 2e-38
Identities = 100/281 (35%), Positives = 155/281 (54%), Gaps = 18/281 (6%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 39 GEAHSLKVEKILFQGKSDYQDVMVFQSTTYGKVLVLDGVIQLTERDECAYQEMITHLPLC 98
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ PNP++VLVIGGGDGG +REV RH VE+ + EID+ V+EVA +F D +
Sbjct: 99 SIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVVEVAKQFFP------DVAVG 152
Query: 130 GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R L IGDGV F+K +D VIVDS+DP+GPA+ LF + F+E AL G+
Sbjct: 153 YEDPRVNLHIGDGVAFLKNVPAGTYDAVIVDSSDPIGPAQELFEKPFFETIARALRPGGV 212
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI-GYASPWA--FLVGIKGD-LDFR 243
TQA S++L + N +F Y++ + Y S L +G +DF+
Sbjct: 213 VSTQAESIWLHMHIIEEIVANCREIFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPAVDFK 272
Query: 244 K----IDLERAKKL--NLKYYDPENHETIFQMPRYVREMLK 278
ID E + LK+Y+ E H+ F +P + + +++
Sbjct: 273 NPINPIDDESHGQTIGPLKFYNSEIHQASFCLPSFAKRVIE 313
>pir||B82842 spermidine synthase XF0143 [imported] - Xylella fastidiosa (strain
9a5c) >gi|9104932|gb|AAF82956.1|AE003868_2 (AE003868)
spermidine synthase [Xylella fastidiosa]
Length = 285
Score = 159 bits (397), Expect = 5e-38
Identities = 92/275 (33%), Positives = 144/275 (51%), Gaps = 14/275 (5%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + F++ K+ E S FQKIE+Y + +G+L+ +DG + L + YHE + HP +
Sbjct: 16 GSAIGFRITGKLDEVQSPFQKIEIYNSTDWGKLMVIDGALMLTSRDNFLYHEMISHPALF 75
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
H P+ V++IGGGD GT+REVL+H ++E+ +IDE+V +A K +
Sbjct: 76 THTAPKCVVIIGGGDCGTLREVLKHPDIEQVTQCDIDEQVTRMAEKHFP------ELCTS 129
Query: 130 GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
RA L+ DGV ++ + + DV+IVDSTDPVGPAK LF+R FYE + AL + G+
Sbjct: 130 NNDPRATLLFSDGVAYMTDCPTNSVDVIIVDSTDPVGPAKGLFNRTFYESCFRALKNDGL 189
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKVY-AYSFPVIGYASP-WAFLVGIK---GDLDF 242
V Q+ S + + MS+ K + FP Y + W+ + K + F
Sbjct: 190 LVQQSESPLALLELIKEMRHEMSKAGFKAFKTLPFPQPCYPTGWWSVTLSSKQPNANFAF 249
Query: 243 RKIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
R+ D + K + YY+ H + P ++ L
Sbjct: 250 RQTDAQ-TKPFDTLYYNAHLHHGVLVPPPFIAHAL 283
>dbj|BAA74542.1| (AB004322) putrescine N-methyltransferase [Nicotiana sylvestris]
Length = 353
Score = 159 bits (397), Expect = 5e-38
Identities = 101/296 (34%), Positives = 162/296 (54%), Gaps = 31/296 (10%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + KV++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 69 EFSALWP-GEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 127
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+VH + + PNP++VL+IGGG G T+ E+LR+ +E+ +VEID+ V++V+ KF
Sbjct: 128 MIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPTIEKIDIVEIDDVVVDVSRKFFPYLA 187
Query: 122 GLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
+ R L++GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 188 ANFND------PRVTLVLGDGAAFVKAAQAEYYDAIIVDSSDPIGPAKDLFERPFFEAVA 241
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWA 231
+AL G+ TQA S++L + N +VF V Y VIGY
Sbjct: 242 KALRPGGVVCTQAESIWLHMHIIKQIIANCRQVFKGSVNYAWTTVPTYPTGVIGY----- 296
Query: 232 FLVGIKG-DLDFRK----IDLE----RAKKLNLKYYDPENHETIFQMPRYVREMLK 278
L +G ++DF+ ID E ++K LK+Y+ + H+ F +P + R M++
Sbjct: 297 MLCSTEGPEIDFKNPVNPIDKETAQVKSKLAPLKFYNSDIHKAAFILPSFARSMIE 352
>gb|AAF14878.1|AF126809_1 (AF126809) putrescine N-methyltransferase [Nicotiana tabacum]
Length = 353
Score = 159 bits (397), Expect = 5e-38
Identities = 101/296 (34%), Positives = 162/296 (54%), Gaps = 31/296 (10%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + KV++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 69 EFSALWP-GEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 127
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+VH + + PNP++VL+IGGG G T+ E+LR+ +E+ +VEID+ V++V+ KF
Sbjct: 128 MIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPTIEKIDIVEIDDVVVDVSRKFFPYLA 187
Query: 122 GLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
+ R L++GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 188 ANFND------PRVTLVLGDGAAFVKAAQAEYYDAIIVDSSDPIGPAKDLFERPFFEAVA 241
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWA 231
+AL G+ TQA S++L + N +VF V Y VIGY
Sbjct: 242 KALRPGGVVCTQAESIWLHMHIIKQIIANCRQVFKGSVNYAWTTVPTYPTGVIGY----- 296
Query: 232 FLVGIKG-DLDFRK----IDLE----RAKKLNLKYYDPENHETIFQMPRYVREMLK 278
L +G ++DF+ ID E ++K LK+Y+ + H+ F +P + R M++
Sbjct: 297 MLCSTEGPEIDFKNPVNPIDKETAQVKSKLAPLKFYNSDIHKAAFILPSFARSMIE 352
>dbj|BAA74544.1| (AB004324) putrescine N-methyltransferase [Nicotiana sylvestris]
Length = 430
Score = 158 bits (396), Expect = 6e-38
Identities = 101/296 (34%), Positives = 162/296 (54%), Gaps = 31/296 (10%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + KV++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 146 EFSALWP-GEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 204
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+VH + + PNP++VL+IGGG G T+ E+LR+ +E+ +VEID+ V++V+ KF
Sbjct: 205 MIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPTIEKIDIVEIDDVVVDVSRKFFPYLA 264
Query: 122 GLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
+ R L++GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 265 ANFND------PRVTLVLGDGAAFVKAAQAGYYDAIIVDSSDPIGPAKDLFERPFFEAVA 318
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWA 231
+AL G+ TQA S++L + N +VF V Y VIGY
Sbjct: 319 KALRPGGVVCTQAESIWLHMHIIKQIIANCRQVFKGSVNYAWTTVPTYPTGVIGY----- 373
Query: 232 FLVGIKG-DLDFRK----IDLE----RAKKLNLKYYDPENHETIFQMPRYVREMLK 278
L +G ++DF+ ID E ++K LK+Y+ + H+ F +P + R M++
Sbjct: 374 MLCSTEGPEVDFKNPINPIDKETTQVKSKLAPLKFYNSDIHKAAFILPSFARSMIE 429
>sp|O82147|SPEE_COFAR SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|3242659|dbj|BAA29033.1| (AB015599) spermidine
synthase [Coffea arabica]
Length = 316
Score = 158 bits (395), Expect = 8e-38
Identities = 99/286 (34%), Positives = 158/286 (54%), Gaps = 29/286 (10%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 37 GEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMIAHLPLC 96
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ P+P++VLVIGGGDGG +REV RH VE+ + EID+ V++V+ +F D +
Sbjct: 97 SIPSPKKVLVIGGGDGGVLREVARHLSVEQIDICEIDKMVVDVSKQFFP------DVAVG 150
Query: 130 GECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R L IGDGV F+K +D +IVDS+DP+GPA+ LF + F+E +AL G+
Sbjct: 151 FEDPRVVLHIGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKPFFESVAKALRPGGV 210
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWAFLVGIKG- 238
TQA S++L + N ++F V Y VIG+ L +G
Sbjct: 211 VCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTTVPTYPSGVIGF-----MLCSTEGP 265
Query: 239 DLDFR----KIDLE--RAKKLN-LKYYDPENHETIFQMPRYVREML 277
+DF+ ID R+K + LK+Y+ E H F +P + ++++
Sbjct: 266 PVDFKHPINPIDANDGRSKTMKPLKFYNSEIHSAAFCLPSFAKKVI 311
>gb|AAF14880.1|AF126811_1 (AF126811) putrescine N-methyltransferase [Nicotiana tabacum]
Length = 381
Score = 158 bits (395), Expect = 8e-38
Identities = 102/297 (34%), Positives = 163/297 (54%), Gaps = 33/297 (11%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + KV++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 97 EFSALWP-GEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 155
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIG-ID 120
+VH + + PNP++VL+IGGG G T+ E+LR+ +E+ +VEID+ V++V+ KF +
Sbjct: 156 MIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPTIEKIDIVEIDDVVVDVSRKFFPYLA 215
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERA 178
D R L++GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 216 ANFSD-------PRVTLVLGDGAAFVKAAQAGYYDAIIVDSSDPIGPAKDLFERPFFEAV 268
Query: 179 YEALNDPGIYVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPW 230
+AL G+ TQA S++L + N +VF V Y VIGY
Sbjct: 269 AKALRPGGVVCTQAESIWLHMHIIKQIIANCRQVFKGSVNYAWTTVPTYPTGVIGY---- 324
Query: 231 AFLVGIKG-DLDFRK----IDLE----RAKKLNLKYYDPENHETIFQMPRYVREMLK 278
L +G ++DF+ ID E ++K LK+Y+ + H+ F +P + R M++
Sbjct: 325 -MLCSTEGPEVDFKNPVNPIDKETTQVKSKLAPLKFYNSDIHKAAFILPSFARSMIE 380
>gb|AAK41051.1| Spermidine synthase [Sulfolobus solfataricus]
Length = 301
Score = 157 bits (394), Expect = 1e-37
Identities = 96/268 (35%), Positives = 160/268 (58%), Gaps = 17/268 (6%)
Query: 17 VKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHPNP-R 75
+++ + E + +Q + + E FG+ L +DG VQ E YHE LVHP++L+ P +
Sbjct: 21 IEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK 80
Query: 76 RVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKG--ECK 133
VL++GGG+G T+REVL+++ VE+A+MV+IDE+VIE A K+ L + +G E K
Sbjct: 81 SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKY-------LYEWHQGAFEDK 133
Query: 134 RAKLIIGDGVEFIKE-NSGFDVVIVDSTDPV--GPAKMLFSREFYERAYEALNDPGIYVT 190
R L+I DG++FIKE +D +I+D TDP+ + ML+++EFYE+ E LN+ G VT
Sbjct: 134 RTNLVITDGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYEKLREILNERGGIVT 193
Query: 191 QAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASPWAFLV-GIKGD-LDFRKIDLE 248
QA S + VT Y + VF + A + + W F+ G++ D L ++D +
Sbjct: 194 QATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVDSK 253
Query: 249 RAKKLN--LKYYDPENHETIFQMPRYVR 274
++++ L++YD +H+ F +P+ ++
Sbjct: 254 INERIDGQLRFYDGYSHKISFSLPKNIK 281
>sp|Q9Y8H7|SPEE_NEUCR SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|5231273|dbj|BAA81738.1| (AB001598) spermidine
synthase [Neurospora crassa]
Length = 291
Score = 157 bits (394), Expect = 1e-37
Identities = 93/277 (33%), Positives = 155/277 (55%), Gaps = 16/277 (5%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ + S +Q + ++++ G +L LD +Q E SY E + H M
Sbjct: 23 GQAMTLKVEKVLHHEKSLYQDVLIFKSTDHGNVLVLDNVIQCTERDEFSYQEMITHLAMN 82
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+HPNP++VLVIGGGDGG +REV++H+ VEEAI+ +IDE VI ++ +F L M
Sbjct: 83 SHPNPKKVLVIGGGDGGVLREVVKHDCVEEAILCDIDEAVIRLSKQF-------LPHMSA 135
Query: 130 G-ECKRAKLIIGDGVEFIKE-NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
G E + K+ +GDG +F+++ + FDV+I DS+DP GPA+ LF + +++ ++AL + G+
Sbjct: 136 GFEHPKVKVHVGDGFKFLEDFKNTFDVIITDSSDPEGPAESLFQKPYFQLLHDALREGGV 195
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVFDKV-YAYSFPVIGYASPWAFLVGIKG-----DLD 241
TQA + +L + ++ VF YAY+ + F+V K +
Sbjct: 196 ITTQAENQWLHLPLITKLKKDCKEVFPVAEYAYTTIPTYPSGQIGFMVCSKDPNANVKVP 255
Query: 242 FRKIDLERAKKLNLKYYDPENHETIFQMPRYVREMLK 278
R E +KL +YY+ E H+ F +P + ++ L+
Sbjct: 256 LRSWSQEEEEKL-CRYYNSEIHKASFILPTFAKKALE 291
>emb|CAB57546.1| (Y18930) putrescine aminopropyl transferase [Sulfolobus
solfataricus]
Length = 301
Score = 157 bits (394), Expect = 1e-37
Identities = 96/268 (35%), Positives = 160/268 (58%), Gaps = 17/268 (6%)
Query: 17 VKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHPNP-R 75
+++ + E + +Q + + E FG+ L +DG VQ E YHE LVHP++L+ P +
Sbjct: 21 IEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK 80
Query: 76 RVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKG--ECK 133
VL++GGG+G T+REVL+++ VE+A+MV+IDE+VIE A K+ L + +G E K
Sbjct: 81 SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKY-------LYEWHQGAFEDK 133
Query: 134 RAKLIIGDGVEFIKE-NSGFDVVIVDSTDPV--GPAKMLFSREFYERAYEALNDPGIYVT 190
R L+I DG++FIKE +D +I+D TDP+ + ML+++EFYE+ E LN+ G VT
Sbjct: 134 RTNLVITDGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYEKLREILNERGGIVT 193
Query: 191 QAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASPWAFLV-GIKGD-LDFRKIDLE 248
QA S + VT Y + VF + A + + W F+ G++ D L ++D +
Sbjct: 194 QATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVDSK 253
Query: 249 RAKKLN--LKYYDPENHETIFQMPRYVR 274
++++ L++YD +H+ F +P+ ++
Sbjct: 254 INERIDGQLRFYDGYSHKISFSLPKNIK 281
>gb|AAF14879.1|AF126810_1 (AF126810) putrescine N-methyltransferase [Nicotiana tabacum]
Length = 375
Score = 157 bits (394), Expect = 1e-37
Identities = 101/296 (34%), Positives = 162/296 (54%), Gaps = 31/296 (10%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + KV++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 91 EFSALWP-GEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 149
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+VH + + PNP++VL+IGGG G T+ E+LR+ +E+ +VEID+ V++V+ KF
Sbjct: 150 MIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPSIEKIDIVEIDDVVVDVSRKFFPYLA 209
Query: 122 GLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
+ R L++GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 210 ANFND------PRVTLVLGDGAAFVKAAQAGYYDAIIVDSSDPIGPAKDLFERPFFEAVA 263
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWA 231
+AL G+ TQA S++L + N +VF V Y VIGY
Sbjct: 264 KALRPGGVVCTQAESIWLHMHIIKQIIANCRQVFKGSVNYAWTTVPTYPTGVIGY----- 318
Query: 232 FLVGIKG-DLDFRK----IDLE----RAKKLNLKYYDPENHETIFQMPRYVREMLK 278
L +G ++DF+ ID E ++K LK+Y+ + H+ F +P + R M++
Sbjct: 319 MLCSTEGPEVDFKNPVNPIDKETTQVKSKLGPLKFYNSDIHKAAFILPSFARSMIE 374
>sp|Q42963|PNMT_TOBAC PUTRESCINE N-METHYLTRANSFERASE (PMT) (A411) >gi|7434030|pir||T03681
putrescine N-methyltransferase (EC 2.1.1.53) A411
[validated] - common tobacco >gi|507825|dbj|BAA05867.1|
(D28506) Putrescine N-Methyltransferase [Nicotiana
tabacum]
Length = 375
Score = 157 bits (393), Expect = 1e-37
Identities = 100/296 (33%), Positives = 163/296 (54%), Gaps = 31/296 (10%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + KV++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 91 EFSALWP-GEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 149
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+VH + + PNP++VL+IGGG G T+ E+LR+ +E+ +VEID+ V++V+ KF
Sbjct: 150 MIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPSIEKIDIVEIDDVVVDVSRKFFPYLA 209
Query: 122 GLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
+ R L++GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 210 ANFND------PRVTLVLGDGAAFVKAAQAGYYDAIIVDSSDPIGPAKDLFERPFFEAVA 263
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFP--------VIGYASPWA 231
+AL G+ TQA S++L + N +VF Y++ VIGY
Sbjct: 264 KALRPGGVVCTQAESIWLHMHIIKQIIANCRQVFKGSVNYAWTTAPTYPTGVIGY----- 318
Query: 232 FLVGIKG-DLDFRK----IDLE----RAKKLNLKYYDPENHETIFQMPRYVREMLK 278
L +G ++DF+ ID E ++K LK+Y+ + H+ F +P + R M++
Sbjct: 319 MLCSTEGPEVDFKNPVNPIDKETTQVKSKLGPLKFYNSDIHKAAFILPSFARSMIE 374
>dbj|BAA82262.1| (AB018571) putrescine N-methyltransferase 2 [Atropa belladonna]
Length = 340
Score = 156 bits (391), Expect = 2e-37
Identities = 100/295 (33%), Positives = 159/295 (53%), Gaps = 30/295 (10%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF + +P G + K+++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 54 EFSQLWP-GEAFSLKIEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 112
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+VH + + P+P++VL+IGGG G T+ EV R+ +E +VEID V++V+ KF
Sbjct: 113 MIVHLPLGSIPSPKKVLIIGGGIGFTLFEVFRYPTIENIDIVEIDNVVVDVSRKFFPYLA 172
Query: 122 GLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
D R L++GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 173 AGFDD------PRVTLVLGDGAAFVKAAQAGYYDAIIVDSSDPIGPAKDLFERPFFEAVA 226
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWA 231
+AL G+ TQA S++L + +N +VF V Y VIGY
Sbjct: 227 KALRPGGVVCTQAESIWLHMHIIKQIIDNCRQVFKGSVNYAWTTVPTYPTGVIGY----- 281
Query: 232 FLVGIKG-DLDFRK----IDLERA---KKLNLKYYDPENHETIFQMPRYVREMLK 278
L KG +DFR ID + + K LK+Y+ + H+ F +P + R +++
Sbjct: 282 MLCSTKGPQVDFRNPVNPIDKKTSHIKSKGPLKFYNSDIHKAAFILPSFARNLME 336
>emb|CAC11499.1| (AL445064) spermidine synthase 2 related protein [Thermoplasma
acidophilum]
Length = 273
Score = 156 bits (391), Expect = 2e-37
Identities = 92/279 (32%), Positives = 157/279 (55%), Gaps = 18/279 (6%)
Query: 3 FIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHES 62
F E Y ++F+V +++ + +Q+I++++T FG+LLA+DGTVQL E YHE
Sbjct: 8 FSERYSDNLQLSFRVSDQLLSIKTDYQRIDLFDTYDFGKLLAIDGTVQLTERDEFIYHEL 67
Query: 63 L-VHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GID 120
+ + P L P L+IGGGDGG R ++ ++ + VEID++V+EV+ +F +
Sbjct: 68 ITMVPYHLTPRPPENALIIGGGDGGAARRLI-DLGLKHIVNVEIDDQVVEVSKRFFPSLS 126
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFIKENS-GFDVVIVDSTDPVGPAKMLFSREFYERAY 179
D KL+I DG++++K S FD++I+DSTDP GPA+ LFS++FY
Sbjct: 127 SAFND-------SHVKLLIQDGIKYVKNTSEKFDLIIIDSTDPEGPAEGLFSKDFYNDTR 179
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASP-WAFLVGIKG 238
+ +N+ + V+Q+GS + + AY M VF V Y+ + Y S W+F V
Sbjct: 180 KIMNEGAVIVSQSGSPFYQPKAIKLAYSGMREVFSDVRVYTGFIPTYPSGFWSFTVASPW 239
Query: 239 DLDFRKIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
+ R +++ KY++ + E F++P++V+E++
Sbjct: 240 TMKPRPVNMSG------KYFNADVMEGSFRLPQFVKELI 272
>gb|AAF14881.1|AF126812_1 (AF126812) putrescine N-methyltransferase [Nicotiana tabacum]
Length = 419
Score = 156 bits (390), Expect = 3e-37
Identities = 102/297 (34%), Positives = 163/297 (54%), Gaps = 33/297 (11%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + KV++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 135 EFSALWP-GEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 193
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIK-FIGID 120
+VH + + PNP++VL+IGGG G T+ E+LR+ +E+ +VEID+ V++V+ K F +
Sbjct: 194 MIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPTIEKIDIVEIDDVVVDVSRKSFPYLA 253
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERA 178
D R L++GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 254 ANFND-------PRVTLVLGDGAAFVKAAQAGYYDAIIVDSSDPIGPAKDLFERPFFEAV 306
Query: 179 YEALNDPGIYVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPW 230
+AL G+ TQA S++L + N +VF V Y VIGY
Sbjct: 307 AKALRPGGVVCTQAESIWLHMHIIKQIIANCRQVFKGSVNYAWTTVPTYPTGVIGY---- 362
Query: 231 AFLVGIKG-DLDFRK----IDLE----RAKKLNLKYYDPENHETIFQMPRYVREMLK 278
L +G ++DF+ ID E ++K LK+Y+ + H+ F +P + R M++
Sbjct: 363 -MLCSTEGPEVDFKNPINPIDKETTQVKSKLAPLKFYNSDIHKAAFILPSFARSMIE 418
>gb|AAB35050.1| spermidine synthase, putrescine aminopropyltransferase, PAPT {EC
2.5.1.6} [rats, Peptide, 297 aa]
Length = 297
Score = 155 bits (389), Expect = 4e-37
Identities = 91/260 (35%), Positives = 147/260 (56%), Gaps = 17/260 (6%)
Query: 13 VAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHP 72
++ +V++ + S++Q I V+ ++ +G +L LDG +Q + E SY E + + + +HP
Sbjct: 33 LSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTEKDEFSYQEMIANLPLCSHP 92
Query: 73 NPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMMKGE 131
NPR+VL+IGGGDGG +REV++H VE + EIDE+VIEV+ KF+ G+ G
Sbjct: 93 NPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIEVSKKFLPGMAIGY-------S 145
Query: 132 CKRAKLIIGDGVEFIKEN-SGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGIYVT 190
+ L +GDG EF+K+N FDV+I DS+DP+GPA+ LF +Y+ AL + GI
Sbjct: 146 SSKLTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGILCC 205
Query: 191 QAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIG-YAS--PWAFLVGIKGDLDFR---- 243
Q +L D L+ + + V +Y++ IG Y S L +FR
Sbjct: 206 QGECQWLHLD-LIKEMRHFCKSLFPVVSYAYCTIGSYGSGEMGLMLCSKNPSTNFREPVQ 264
Query: 244 KIDLERAKKLNLKYYDPENH 263
++ + +++ LKYY+ + H
Sbjct: 265 QLRQAQVEQMQLKYYNSDMH 284
>gb|AAD02231.1| (AF043108) spermidine synthase 1 [Pisum sativum]
Length = 334
Score = 155 bits (388), Expect = 5e-37
Identities = 99/282 (35%), Positives = 155/282 (54%), Gaps = 22/282 (7%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +QL E +Y E + H +
Sbjct: 56 GEAHSLKVEKILFQGKSDYQDVMVFQSATYGKVLILDGVIQLTERDECAYQEMITHLPLC 115
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIK-FIGIDGGLLDKMM 128
+ PNP++VLVIGGGDGG +REV RH VE+ + EID+ V++V+ + F I G D
Sbjct: 116 SIPNPKKVLVIGGGDGGVLREVARHSSVEKIDICEIDKMVVDVSKEYFPDIAVGFAD--- 172
Query: 129 KGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPG 186
R L IGDGV F+K +D VIVDS+DP+GPA+ LF + F+E AL G
Sbjct: 173 ----PRVTLNIGDGVAFLKAAPEGTYDAVIVDSSDPIGPAQELFEKPFFESVARALRPGG 228
Query: 187 IYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI-GYASPWA--FLVGIKG-DLDF 242
+ TQA S++L + N +VF Y++ + Y S L +G +DF
Sbjct: 229 VVCTQAESIWLHMHIIEDIVVNCRQVFKGSVNYAWTTVPTYPSGMIGFMLCSTEGPSVDF 288
Query: 243 R-------KIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
+ + D ++A + LK+Y+ E H F +P + + +
Sbjct: 289 KHPVNPIDENDSQQAAR-PLKFYNREIHSAAFCLPSFAKRAI 329
>gb|AAF01311.1|AF184093_1 (AF184093) spermine synthase [Arabidopsis thaliana]
>gi|6013269|gb|AAF01312.1|AF184094_1 (AF184094) spermine
synthase [Arabidopsis thaliana]
Length = 339
Score = 155 bits (387), Expect = 7e-37
Identities = 80/242 (33%), Positives = 138/242 (56%), Gaps = 12/242 (4%)
Query: 14 AFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHPN 73
+F + + +G S++Q I + +T+ FG++L +DG +Q E YHE L+HP +L HPN
Sbjct: 47 SFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPN 106
Query: 74 PRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGECK 133
P+ V ++GGG+G RE+L+H +E+ +M +ID+EV++ +F+ ++ K
Sbjct: 107 PKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCN------K 160
Query: 134 RAKLIIGDG-VEFIKENSGFDVVIVDSTDPV--GPAKMLFSREFYERAYE-ALNDPGIYV 189
+ +L+I D E K FD+++ D DPV GP L+++ FY+ + L+ GI+V
Sbjct: 161 KLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFV 220
Query: 190 TQAGSVYLFTDELV--TAYENMSRVFDKVYAYSFPVIGYASPWAFLVGIKGDLDFRKIDL 247
TQAG +FT + V + Y M +VF V AY+ V +A W +++ + D ++
Sbjct: 221 TQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFADTWGWVMASDHEFDVEVDEM 280
Query: 248 ER 249
+R
Sbjct: 281 DR 282
>gi|6325326 putrescine aminopropyltransferase (spermidine synthase); Spe3p
[Saccharomyces cerevisiae]
>gi|2498945|sp|Q12074|SPEE_YEAST SPERMIDINE SYNTHASE
(PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|1084791|pir||S54090 spermidine synthase (EC
2.5.1.16) SPE3 - yeast (Saccharomyces cerevisiae)
>gi|805049|emb|CAA89186.1| (Z49219) unknown
[Saccharomyces cerevisiae] >gi|1230677|gb|AAB68120.1|
(U51033) Also found in EMBL accession number Z49219
[Saccharomyces cerevisiae] >gi|1314137|emb|CAA94977.1|
(Z71255) unknown [Saccharomyces cerevisiae]
>gi|1326074|gb|AAC17191.1| (U27519) spermidine synthase
[Saccharomyces cerevisiae]
Length = 293
Score = 154 bits (385), Expect = 1e-36
Identities = 92/276 (33%), Positives = 152/276 (54%), Gaps = 16/276 (5%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ + SK+Q + ++++ +G +L LD +Q E +Y E + H +
Sbjct: 25 GQAMTLKVEKVLHHEKSKYQDVLIFKSTTYGNVLVLDNVIQATERDEFAYQEMIAHLALN 84
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+HPNP++VLVIGGGDGG +REV++H+ VEEA + +IDE VI ++ ++ L +
Sbjct: 85 SHPNPKKVLVIGGGDGGVLREVVKHDSVEEAWLCDIDEAVIRLSKEY------LPEMAAS 138
Query: 130 GECKRAKLIIGDGVEFIKE-NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGIY 188
+ K IGDG +F+++ + FDV+I DS+DP GPA+ LF +E+++ AL + G+
Sbjct: 139 YSHPKVKTHIGDGFQFLRDYQNTFDVIITDSSDPEGPAETLFQKEYFQLLNSALTEKGVI 198
Query: 189 VTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGY--ASPWAFLVGIKGDL-----D 241
TQA S+++ + + S VF V YSF I F+V K
Sbjct: 199 TTQAESMWIHLPIIKDLKKACSEVF-PVAEYSFVTIPTYPTGTIGFMVCSKDKTCNVKKP 257
Query: 242 FRKIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
R+I E+ +L +YY+ + HE F +P + + L
Sbjct: 258 LREISDEKEAEL-YRYYNKKIHEASFVLPTWAAKEL 292
>sp|P57305|SPEE_BUCAI SPERMIDINE SYNTHASE (PUTRESCINE AMINOPROPYLTRANSFERASE) (SPDSY)
>gi|10038891|dbj|BAB12926.1| (AP001118) spermidine
synthase [Buchnera sp. APS]
Length = 286
Score = 154 bits (385), Expect = 1e-36
Identities = 88/278 (31%), Positives = 145/278 (51%), Gaps = 15/278 (5%)
Query: 6 WYPRGY---GVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHES 62
W+ + Y G F +++ +++ + ++ +++ FG+++ +D VQ E YHE
Sbjct: 7 WHEKLYCHLGQYFLIEKMLYKKKTPHHQVMIFKNSVFGKIMVIDDIVQTTERDEFIYHEM 66
Query: 63 LVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGG 122
L H ++AH + + VL+IGGGDGG +REV R++ +E MVEID +I++ K+
Sbjct: 67 LTHVPIIAHGSIKSVLIIGGGDGGILREVCRYKMIENITMVEIDVNIIDLCKKYFPNHSN 126
Query: 123 LLDKMMKGECKRAKLIIGDGVEFIKE-NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEA 181
+ R LII DG+ FIK FD++I DSTDP+G K LF EFY
Sbjct: 127 -----QAYQDSRLNLIIDDGLNFIKRTKEKFDLIISDSTDPIGCGKNLFRSEFYFNCKNH 181
Query: 182 LNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYASPWAFLVGIKGDLD 241
L + GI+V Q G +L +E + Y+N+ + F Y V Y +++
Sbjct: 182 LEENGIFVAQNGVFFLQKNETILTYKNLKKYFYDTRFYQANVPTYYGGVMVFAWGTNNIE 241
Query: 242 FRKIDLERAK------KLNLKYYDPENHETIFQMPRYV 273
+RK LE+ + KL+ YY+ + H + F +P+Y+
Sbjct: 242 YRKNSLEKIQIRIKNTKLDFNYYNAKIHISSFYLPQYI 279
>dbj|BAA82263.1| (AB018572) putrescine N-methyltransferase [Hyoscyamus niger]
Length = 338
Score = 153 bits (383), Expect = 2e-36
Identities = 98/290 (33%), Positives = 156/290 (53%), Gaps = 20/290 (6%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + K+++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 55 EFSALWP-GEAFSLKIEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 113
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+VH + + P+P++VL+IGGG G T+ EV R+ +E+ +VEID+ VI+V+ K+
Sbjct: 114 MIVHLPLGSIPSPKKVLIIGGGIGFTLFEVSRYSTIEKIDIVEIDDVVIDVSRKYFPYLA 173
Query: 122 GLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
D R LI+GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 174 AGFDD------PRVTLIVGDGAAFVKAAQPGYYDAIIVDSSDPIGPAKDLFERPFFEAVT 227
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGY--ASP 229
+AL G+ TQA S++L + N +VF V Y VIGY S
Sbjct: 228 KALRPGGVVCTQAESIWLHMHLIKQIIANCRQVFKGSVNYAWTTVPTYPTGVIGYMLCST 287
Query: 230 WAFLVGIKGDLDFRKIDLERAK-KLNLKYYDPENHETIFQMPRYVREMLK 278
V K ++ D K K LK+Y+ + H+ F +P + R++++
Sbjct: 288 EGPEVNFKNPVNSIDKDTTHVKSKGPLKFYNSDIHKAAFILPSFARDLVE 337
>dbj|BAB08415.1| (AB025622) spermidine synthase [Arabidopsis thaliana]
Length = 343
Score = 153 bits (383), Expect = 2e-36
Identities = 97/290 (33%), Positives = 158/290 (54%), Gaps = 29/290 (10%)
Query: 3 FIEWYPR-GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
F E +PR G + KV++ +F+ S FQ++ V+E+ +G++L LDG VQL + E +Y E
Sbjct: 56 FSEPHPRSGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQE 115
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKF----- 116
+ H + + +P+ VLV+GGGDGG +RE+ RH VE + EID+ VI+V+ KF
Sbjct: 116 MIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVIDVSKKFFPELA 175
Query: 117 IGIDGGLLDKMMKGECKRAKLIIGDGVEFIKEN--SGFDVVIVDSTDPVGPAKMLFSREF 174
+G D R +L IGD EF++++ +D +IVDS+DPVGPA L + F
Sbjct: 176 VGFDD-----------PRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 224
Query: 175 YERAYEALNDPGIYVTQAGSVYLFT---DELVT----AYENMSRVFDKVYAYSFPVIGY- 226
+E AL G+ A S++L T +++++ ++++ + V Y VIG+
Sbjct: 225 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTYPSGVIGFV 284
Query: 227 -ASPWAFLVGIKGDLD-FRKIDLERAKKLNLKYYDPENHETIFQMPRYVR 274
S V K ++ K+D K LK+Y+ + H F +P ++R
Sbjct: 285 LCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLR 334
>dbj|BAA82261.1| (AB018570) putrescine N-methyltransferase 1 [Atropa belladonna]
>gi|5381174|dbj|BAA82264.1| (AB018573) putrescine
N-methyltransferase 1 [Atropa belladonna]
Length = 336
Score = 151 bits (378), Expect = 8e-36
Identities = 97/290 (33%), Positives = 155/290 (53%), Gaps = 20/290 (6%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + K+++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 53 EFSALWP-GEAFSLKIEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 111
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDG 121
+VH + + P+P++VL+IGGG G T+ EV R+ ++ +VEID+ V++V+ K+
Sbjct: 112 MIVHLPLGSIPSPKKVLIIGGGIGFTLFEVSRYPTIDTIDIVEIDDVVVDVSRKYFPYLA 171
Query: 122 GLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERAY 179
D R LIIGDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 172 AGFDD------PRVTLIIGDGAAFVKAAQPGYYDAIIVDSSDPIGPAKDLFERPFFEAVA 225
Query: 180 EALNDPGIYVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGY--ASP 229
+AL G+ TQA S++L + N +VF V Y VIGY S
Sbjct: 226 KALRPGGVVCTQAESIWLHMHLIKQIIANCRQVFKGSVNYAWTTVPTYPTGVIGYMLCST 285
Query: 230 WAFLVGIKGDLDFRKIDLERAK-KLNLKYYDPENHETIFQMPRYVREMLK 278
V K ++ D K K LK+Y+ + H+ F +P + R++++
Sbjct: 286 EGPEVNFKNPVNSIDKDTSHVKSKGPLKFYNSDIHKAAFILPSFARDLVE 335
>gi|6323175 Spermine Synthase; Spe4p [Saccharomyces cerevisiae]
>gi|6094342|sp|Q12455|SPSY_YEAST SPERMINE SYNTHASE
(SPERMIDINE AMINOPROPYLTRANSFERASE) (SPMSY)
>gi|2131789|pir||S64995 probable spermidine synthase (EC
2.5.1.16) YLR146c - yeast (Saccharomyces cerevisiae)
>gi|1262306|gb|AAB82380.1| (U53879) Ylr146cp: spermidine
synthase [Saccharomyces cerevisiae]
>gi|1360572|emb|CAA97718.1| (Z73318) ORF YLR146c
[Saccharomyces cerevisiae] >gi|3201943|gb|AAC19368.1|
(AF067970) spermine synthase [Saccharomyces cerevisiae]
Length = 300
Score = 151 bits (377), Expect = 1e-35
Identities = 96/283 (33%), Positives = 150/283 (52%), Gaps = 24/283 (8%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G V ++E S+FQ I ++ + +G +L LDG VQ E +Y E + H M
Sbjct: 25 GQAFTMTVDSILYEARSEFQDILIFRNKVYGTVLVLDGIVQCTEFDEFAYQEMITHIAMF 84
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMM 128
AH NP+RVL+IGGGDGG +REV +H VE+ MVEID VIE++ KF+ + G D
Sbjct: 85 AHSNPKRVLIIGGGDGGVLREVAKHSCVEDITMVEIDSSVIELSRKFLPTLSNGAFDD-- 142
Query: 129 KGECKRAKLIIGDGVEFIKE------NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEAL 182
+R L + DG +F+++ + FDV+I DS+DP GPA+ F ++E +AL
Sbjct: 143 ----ERLDLKLCDGFKFLQDIGASDVHKKFDVIITDSSDPEGPAEAFFQERYFELLKDAL 198
Query: 183 NDPGIYVTQAGSVYLFTDELVTAYENMS-RVFDKV-YAYSFPVIGYASPWAFLV------ 234
N G+ + Q+ + + + +N + +VF Y Y+ V Y S L+
Sbjct: 199 NPNGVVIMQSSENFWLNLKYLHDLKNTAKKVFPNTEYCYTM-VPTYTSGQLGLIVCSNNA 257
Query: 235 GIKGDLDFRKIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
I ++ RKI + K LKYY+P+ H + F +P + +++
Sbjct: 258 NIPLNIPQRKISEQEQGK--LKYYNPQIHSSAFVLPTWADKVI 298
>emb|CAB61629.1| (AJ251298) spermidine synthase 1 [Oryza sativa]
Length = 323
Score = 150 bits (375), Expect = 2e-35
Identities = 94/286 (32%), Positives = 150/286 (51%), Gaps = 29/286 (10%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVML 69
G + KV++ +F+G S +Q + V+++ +G++L LDG +Q+ E +Y E + H +
Sbjct: 44 GEAHSLKVEKVLFQGKSDYQNVMVFQSSTYGKVLVLDGVIQVTERDECAYQEMITHLPLC 103
Query: 70 AHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMK 129
+ +P++VLVIGGGDGG +REV RH VE+ + EID+ V++V+ +F +
Sbjct: 104 SIKDPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVVDVSKQFFP------HLAVG 157
Query: 130 GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERAYEALNDPGI 187
E R L IGDGV F+K +D VIVDS+DP+GPA+ LF + F++ AL G+
Sbjct: 158 FEDPRVSLHIGDGVAFLKNAPEGTYDAVIVDSSDPIGPAQELFEKPFFQSVARALRPGGV 217
Query: 188 YVTQAGSVYLFTDELVTAYENMSRVF--------DKVYAYSFPVIGYASPWAFLVGIKG- 238
TQA S++L + N +VF V Y VIG+ L +G
Sbjct: 218 VCTQAESIWLHMHIIEDIVANCRQVFKGSVNYAWTTVPTYPSGVIGF-----MLCSTEGP 272
Query: 239 DLDFR-------KIDLERAKKLNLKYYDPENHETIFQMPRYVREML 277
+DF+ + K LK+Y+ E H F +P + + ++
Sbjct: 273 TVDFQHPIFNIEDNEFSTKSKGPLKFYNSEIHSASFCLPSFAKRVI 318
>dbj|BAA74543.1| (AB004323) putrescine N-methyltransferase [Nicotiana sylvestris]
Length = 319
Score = 143 bits (358), Expect = 2e-33
Identities = 81/226 (35%), Positives = 131/226 (57%), Gaps = 11/226 (4%)
Query: 2 EFIEWYPRGYGVAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHE 61
EF +P G + KV++ +F+G S +Q + ++E+ +G++L LDG +Q G Y E
Sbjct: 97 EFSALWP-GEAFSLKVEKLLFQGKSDYQDVMLFESATYGKVLTLDGAIQHTENGGFPYTE 155
Query: 62 SLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIG-ID 120
+VH + + PNP++VL+IGGG G T+ E+LR+ +E+ +VEID+ V++V+ KF +
Sbjct: 156 MIVHLPLGSIPNPKKVLIIGGGIGFTLFEMLRYPTIEKIDIVEIDDVVVDVSRKFFPYLA 215
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDSTDPVGPAKMLFSREFYERA 178
D R L++GDG F+K + +D +IVDS+DP+GPAK LF R F+E
Sbjct: 216 ANFSD-------PRVTLVLGDGAAFVKAAQAGYYDAIIVDSSDPIGPAKDLFERPFFEAV 268
Query: 179 YEALNDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI 224
+AL G+ TQA S++L + N +VF Y++ +
Sbjct: 269 AKALRPGGVVCTQAESIWLHMHIIKQIIANCRQVFKGSVNYAWTTV 314
>pir||D83048 hypothetical protein PA4774 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9951039|gb|AAG08160.1|AE004891_2
(AE004891) hypothetical protein [Pseudomonas aeruginosa]
Length = 349
Score = 139 bits (346), Expect = 4e-32
Identities = 90/277 (32%), Positives = 145/277 (51%), Gaps = 22/277 (7%)
Query: 15 FKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHPNP 74
++++ +++G +++Q + + +T + R+L LDG +Q E YHE LV P MLAH P
Sbjct: 46 YRLRRVLYQGRTRWQNVLIADTYNYDRVLMLDGAIQSAESDESLYHELLVQPAMLAHDEP 105
Query: 75 RRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIK--FIGIDGGLLDKMMKGEC 132
R VL+IGGG+G T+REVL H V A+MV++D E++E+ + F G D
Sbjct: 106 RDVLIIGGGEGATLREVLSHASVRRAVMVDLDRELVELCREHLFQWHQGAFDD------- 158
Query: 133 KRAKLIIGDGVEFI-KENSGFDVVIVDSTDPV--GPAKMLFSREFYERAYEALNDPGIYV 189
R +L+ DG ++ ++ S +DVVI+D D + GPA+ L++R+FYE + L G+
Sbjct: 159 PRCELLAEDGRAYLERDPSLYDVVIIDVVDMLDNGPAQALYTRQFYELLHSRLRPGGVVA 218
Query: 190 TQAGSVYLFTDELVTA--YENMSRVFDKVYAYSFPVIGYASPWAFLVGI----KGDLDFR 243
Q G + +D+ A + VF +V++Y V + S W FL+
Sbjct: 219 VQ-GLEFSHSDDKPHAALARTLRSVFSQVHSYRATVPSFLSSWGFLLASDWLDTNHWQAE 277
Query: 244 KIDLERAKKLN---LKYYDPENHETIFQMPRYVREML 277
ID +KL L + D + + F M R R +L
Sbjct: 278 DIDRRIERKLGPLWLDHLDGDYLKACFVMDRETRFLL 314
>gb|AAG24612.1| (AF298195) spermidine synthase [Leishmania donovani]
Length = 300
Score = 128 bits (319), Expect = 7e-29
Identities = 90/286 (31%), Positives = 141/286 (48%), Gaps = 27/286 (9%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEG---FGRLLALDGTVQLVTEGEESYHESLVHP 66
G KV++ +++ ++FQ + V+E++ +G ++ LDG +QL E YHE L +
Sbjct: 21 GQAQGLKVEKVLYDQPTEFQHLTVFESDPSGPWGTVMTLDGAIQLTDYDEFVYHEMLANL 80
Query: 67 VMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAI-----MVEIDEEVIEVAIK-FIGID 120
+ H P RVL+IGGGDGG +REVLRH+ ++ I +V+ID VI+ + K F I
Sbjct: 81 SLTCHHKPERVLIIGGGDGGVVREVLRHKSEKDGIVQSVELVDIDGAVIQQSKKHFPQIA 140
Query: 121 GGLLDKMMKGECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREFYERA 178
G + + +GDG F+K +S +DV+I+D+TDP GPA LF +FY
Sbjct: 141 CGFANPCVTA-------TVGDGAAFVKRAPDSVYDVIIIDTTDPKGPASELFGADFYTNV 193
Query: 179 YEALNDPGIYVTQAGSVYLFTD--ELVTAYENMSRVFDKV-YAYSF-PVIGYASPWAFLV 234
L G+ Q SV+L E++ + F V YA + P S +
Sbjct: 194 LRILRPGGVLCNQGESVWLHRPLIEMMMGFLKKDIGFATVNYAMIYIPTYPCGSIGTLVC 253
Query: 235 GIKGDLDFRKIDLERAKKL----NLKYYDPENHETIFQMPRYVREM 276
D D + + + L LKYY + H+ F +PR+ +
Sbjct: 254 AKSADTDV-TVPMRPVESLGFADQLKYYSSDMHKAAFVLPRFAAHL 298
>emb|CAB42358.1| (AL035213) spermidine synthase 1 [Leishmania major]
Length = 300
Score = 125 bits (312), Expect = 4e-28
Identities = 86/290 (29%), Positives = 139/290 (47%), Gaps = 35/290 (12%)
Query: 10 GYGVAFKVKEKIFEGHSKFQKIEVYETEG---FGRLLALDGTVQLVTEGEESYHESLVHP 66
G KV++ +++ ++FQ + V+E++ +G ++ LDG +QL E YHE L +
Sbjct: 21 GQAQGLKVEKVLYDQPTEFQHLTVFESDPSGPWGTVMTLDGAIQLTDYDEFVYHEMLANL 80
Query: 67 VMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDK 126
+ H P RVLVIGGGDGG +REVLRH+ ++ ++ +++ + IDG ++ +
Sbjct: 81 SLACHHKPERVLVIGGGDGGVVREVLRHKSEKDGVV---------QSVELVDIDGAVMQQ 131
Query: 127 MMK----------GECKRAKLIIGDGVEFIKE--NSGFDVVIVDSTDPVGPAKMLFSREF 174
K C A +GDG F++ +S +DV+I+D+TDP GPA LF +F
Sbjct: 132 SKKHFPQVACGFANPCVTA--TVGDGAAFVRNVPDSVYDVIIIDTTDPKGPASELFGADF 189
Query: 175 YERAYEALNDPGIYVTQAGSVYLFTD--ELVTAYENMSRVFDKV-YAYSF-PVIGYASPW 230
Y L G+ Q SV+L E + + F V YA + P S
Sbjct: 190 YTNVLRILRPGGVVCNQGESVWLHRPLIEKMMGFLKKDIGFATVKYAMIYIPTYPCGSIG 249
Query: 231 AFLVGIKGDLDFRKIDLERAKKL----NLKYYDPENHETIFQMPRYVREM 276
+ D D + + + L LKYY + H+ F +PR+ +
Sbjct: 250 TLVCAKSADTDV-TVPMRPVESLGFADQLKYYSSDMHKAAFVLPRFAAHL 298
>pir||H70886 probable spermidine synthase - Mycobacterium tuberculosis (strain
H37RV) >gi|3261489|emb|CAA15488.1| (AL008687) speE
[Mycobacterium tuberculosis]
Length = 523
Score = 100 bits (246), Expect = 2e-20
Identities = 71/178 (39%), Positives = 95/178 (52%), Gaps = 15/178 (8%)
Query: 25 HSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHPNPRRVLVIGGGD 84
HS +Q+I V G L LDG +Q T E Y ESLV+P + R VLV+GGGD
Sbjct: 257 HSAYQEI-VVTRRGDDLRLYLDGGLQFCTRDEYRYTESLVYPAV--SDGARSVLVLGGGD 313
Query: 85 GGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFI-GIDGGLLDKMMKGECKRAKLIIGDGV 143
G RE+LR +E+ + VE+D VIE+A + ++ G LD R ++I D +
Sbjct: 314 GLAARELLRQPGIEQIVQVELDPAVIELARTTLRDVNAGSLDN------PRVHVVIDDAM 367
Query: 144 EFIK----ENSGFDVVIVDSTDPVGPA-KMLFSREFYERAYEALNDPGIYVTQAGSVY 196
+++ +GFD VIVD DP P L+S EFY A AL G+ V QAGS Y
Sbjct: 368 SWLRGAAVPPAGFDAVIVDLRDPDTPVLGRLYSTEFYALAARALAPGGLMVVQAGSPY 425
>gb|AAC14108.1| (AF055854) spermidine synthase [Synechococcus PCC7002]
Length = 125
Score = 95.2 bits (233), Expect = 8e-19
Identities = 43/91 (47%), Positives = 64/91 (70%)
Query: 26 SKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHPNPRRVLVIGGGDG 85
++FQ++ + E+ +G+ L LDG Q T E YHE LVHP M+AH P++VL++G G+G
Sbjct: 35 TQFQEMAIVESGAYGKALVLDGKWQSCTGDEFLYHEPLVHPAMIAHGAPKKVLILGAGEG 94
Query: 86 GTIREVLRHEEVEEAIMVEIDEEVIEVAIKF 116
T+REVLR + VE+ +MV+ID EV+E +F
Sbjct: 95 ATVREVLRWQSVEKVMMVDIDGEVVEACKEF 125
>pir||B70417 conserved hypothetical protein aq_1350 - Aquifex aeolicus
>gi|2983778|gb|AAC07337.1| (AE000736) hypothetical
protein [Aquifex aeolicus]
Length = 493
Score = 90.9 bits (222), Expect = 2e-17
Identities = 75/248 (30%), Positives = 122/248 (48%), Gaps = 19/248 (7%)
Query: 15 FKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHPNP 74
F +E I + +QKI V G L LDG +Q T E+ YHE+LVH
Sbjct: 219 FYGEEIIHFEQTPYQKI-VLTRFGKHYSLYLDGHLQFSTLDEKRYHETLVHVPASFLKRY 277
Query: 75 RRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGEC-- 132
+ L++GGGDG +RE LR E +V++D ++IE + K + ++ K+ +
Sbjct: 278 EKALILGGGDGLALRE-LRKYPFGEIHLVDLDPKMIEFSKKNL-----VMRKINENSFYD 331
Query: 133 KRAKLIIGDGVEFIKE-NSGFDVVIVDSTDPVGPAK-MLFSREFYERAYEALNDPGIYVT 190
R K+ D F+K+ +D VIVD DP P+ ++S EFY L + GI++T
Sbjct: 332 TRLKVFSEDAFNFVKKTKEKYDFVIVDLIDPRTPSSARVYSLEFYMSLKNKLKEDGIFIT 391
Query: 191 QAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVIGYA---SPWAFLVGIKGDLDFRKIDL 247
QAG + + + ++ + K Y++P++ Y W ++G K L+F +L
Sbjct: 392 QAGDTFYKRE----VFCSILKTIKKAGFYAYPLVVYIPTFGEWGMVIGSKEPLNFENFEL 447
Query: 248 -ERAKKLN 254
E+ + LN
Sbjct: 448 KEKTEFLN 455
>emb|CAB86120.1| (AL163003) putative spermidine synthase [Streptomyces coelicolor
A3(2)]
Length = 531
Score = 84.7 bits (206), Expect = 1e-15
Identities = 59/159 (37%), Positives = 86/159 (53%), Gaps = 13/159 (8%)
Query: 43 LALDGTVQLVTEGEESYHESLVHPVMLA-HPNPRRVLVIGGGDGGTIREVLRHEEVEEAI 101
L L+G +Q + E YHESLVHP + H N VL++GGGDG +REVLRHE V
Sbjct: 281 LFLNGDLQFSSVDEYRYHESLVHPALSGPHSN---VLIMGGGDGLALREVLRHEGVRHVT 337
Query: 102 MVEIDEEVIEVAIKFIGIDGGLLDKMMKG--ECKRAKLIIGDGVEFIKE-NSGFDVVIVD 158
+VE+D + +A F L K+ +G + RAK++ D +++E +D VI+D
Sbjct: 338 LVELDPAMTRLARTF-----EPLRKLNQGSLDDPRAKVVNADAFTWLREARQQYDAVIID 392
Query: 159 STDPVGPA-KMLFSREFYERAYEALNDPGIYVTQAGSVY 196
DP + L+S EFY+ L G + Q+GS +
Sbjct: 393 FPDPDSASLAKLYSVEFYDLLRRVLAPDGQVMVQSGSPF 431
>gb|AAF49935.1| (AE003542) CG4300 gene product [alt 2] [Drosophila melanogaster]
Length = 367
Score = 82.7 bits (201), Expect = 5e-15
Identities = 62/224 (27%), Positives = 114/224 (50%), Gaps = 20/224 (8%)
Query: 13 VAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHP 72
+ + + + +FE S FQKI++ ++ G +L LD +Q + E + Y E+L+ + +
Sbjct: 137 IEYDIDKVVFEARSPFQKIQIMHSKTLGNMLLLD-ELQNIAESDLIYTETLMCRGVENYE 195
Query: 73 NPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGEC 132
+ + ++GGGDG + E+L+ E + +M+EIDE V++ K++ + G + + KG+
Sbjct: 196 G-KEICILGGGDGALLYELLK-ENPKHVVMLEIDELVMQTCNKYLNVICGDVLEKRKGD- 252
Query: 133 KRAKLIIGDGVEFIK----ENSGFDVVIVDSTD-PVGPA----KMLFSREFYERAYEALN 183
+ ++I+GD VE++K E FD V D TD P+ A F R +E +++ L
Sbjct: 253 -QYEIIVGDCVEYLKKFIAEGRKFDYVFGDLTDIPITDAPEGETWDFIRTIFEHSFKVLK 311
Query: 184 DPGIYVTQAG------SVYLFTDELVTAYENMSRVFDKVYAYSF 221
G Y+T + LF ++L + K + SF
Sbjct: 312 PDGKYLTHGNGSTCKVQLRLFEEQLNLLRPKVKFTTTKAFVPSF 355
>gb|AAF49934.1| (AE003542) CG4300 gene product [alt 1] [Drosophila melanogaster]
Length = 366
Score = 82.7 bits (201), Expect = 5e-15
Identities = 62/224 (27%), Positives = 114/224 (50%), Gaps = 20/224 (8%)
Query: 13 VAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHP 72
+ + + + +FE S FQKI++ ++ G +L LD +Q + E + Y E+L+ + +
Sbjct: 136 IEYDIDKVVFEARSPFQKIQIMHSKTLGNMLLLD-ELQNIAESDLIYTETLMCRGVENYE 194
Query: 73 NPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGEC 132
+ + ++GGGDG + E+L+ E + +M+EIDE V++ K++ + G + + KG+
Sbjct: 195 G-KEICILGGGDGALLYELLK-ENPKHVVMLEIDELVMQTCNKYLNVICGDVLEKRKGD- 251
Query: 133 KRAKLIIGDGVEFIK----ENSGFDVVIVDSTD-PVGPA----KMLFSREFYERAYEALN 183
+ ++I+GD VE++K E FD V D TD P+ A F R +E +++ L
Sbjct: 252 -QYEIIVGDCVEYLKKFIAEGRKFDYVFGDLTDIPITDAPEGETWDFIRTIFEHSFKVLK 310
Query: 184 DPGIYVTQAG------SVYLFTDELVTAYENMSRVFDKVYAYSF 221
G Y+T + LF ++L + K + SF
Sbjct: 311 PDGKYLTHGNGSTCKVQLRLFEEQLNLLRPKVKFTTTKAFVPSF 354
>pir||T36545 probable spermidine synthase - Streptomyces coelicolor
>gi|4753879|emb|CAB42042.1| (AL049754) putative
spermidine synthase [Streptomyces coelicolor A3(2)]
Length = 554
Score = 77.3 bits (187), Expect = 2e-13
Identities = 57/156 (36%), Positives = 83/156 (52%), Gaps = 8/156 (5%)
Query: 43 LALDGTVQLVTEGEESYHESLVHPVMLAHPNPRRVLVIGGGDGGTIREVLRHEEVEEAIM 102
L LDG +++ E SYHE+LVHP M P+ R VL++GGGDG REVLRH V +
Sbjct: 296 LFLDGRLRVRGSDERSYHEALVHPAM-GGPHAR-VLILGGGDGLAAREVLRHPGVRRVDV 353
Query: 103 VEIDEEVIEVAIKFIGIDGGLLDKMMKGECKRAKLIIGDGVEFIK--ENSGFDVVIVDST 160
+E D + +A G+ L++ G+ R +++ GD ++ ++ +DVVI D
Sbjct: 354 LEADPGLARLARHDPGL--STLNEHAYGDA-RVRVLSGDAFHRLRATPSATYDVVISDLP 410
Query: 161 DP-VGPAKMLFSREFYERAYEALNDPGIYVTQAGSV 195
DP V + L+S+EFY L G AG V
Sbjct: 411 DPGVTASTKLYSQEFYGLLRRVLAPDGRLAVHAGPV 446
>gi|4759152 spermine synthase; SpS [Homo sapiens] >gi|1082797|pir||S54160
spermine synthase (EC 2.5.1.22) - human
>gi|791051|emb|CAA88921.1| (Z49099) spermine synthase
[Homo sapiens]
Length = 368
Score = 74.5 bits (180), Expect = 1e-12
Identities = 65/233 (27%), Positives = 114/233 (48%), Gaps = 20/233 (8%)
Query: 13 VAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHP 72
V + + E +++ S +Q I++ ++ FG +L L G V L E + +Y +++ +
Sbjct: 134 VEYDIDEVVYDEDSPYQNIKILHSKQFGNILILSGDVNLA-ESDLAYTRAIMGSGKEDYT 192
Query: 73 NPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGEC 132
+ VL++GGGDGG + E+++ + + MVEID+ VI+ K++ G + +KG+C
Sbjct: 193 G-KDVLILGGGDGGILCEIVKLKP-KMVTMVEIDQMVIDGCKKYMRKTCGDVLDNLKGDC 250
Query: 133 KRAKLIIGDGV----EFIKENSGFDVVIVD------STDPVGPAKMLFSREFYERAYEAL 182
+++I D + + KE FD VI D ST P + F R + + + L
Sbjct: 251 --YQVLIEDCIPVLKRYAKEGREFDYVINDLTAVPISTSPEEDSTWEFLRLILDLSMKVL 308
Query: 183 NDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI---GYASPWAF 232
G Y TQ V L T+ L E + R++ V +S ++ Y W F
Sbjct: 309 KQDGKYFTQGNCVNL-TEALSLYEEQLGRLYCPV-EFSKEIVCVPSYLELWVF 359
>sp|P52788|SPSY_HUMAN SPERMINE SYNTHASE (SPERMIDINE AMINOPROPYLTRANSFERASE) (SPMSY)
>gi|2198557|gb|AAB61308.1| (AD001528) spermidine
aminopropyltransferase [Homo sapiens]
Length = 366
Score = 74.5 bits (180), Expect = 1e-12
Identities = 65/233 (27%), Positives = 114/233 (48%), Gaps = 20/233 (8%)
Query: 13 VAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHP 72
V + + E +++ S +Q I++ ++ FG +L L G V L E + +Y +++ +
Sbjct: 132 VEYDIDEVVYDEDSPYQNIKILHSKQFGNILILSGDVNLA-ESDLAYTRAIMGSGKEDYT 190
Query: 73 NPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGEC 132
+ VL++GGGDGG + E+++ + + MVEID+ VI+ K++ G + +KG+C
Sbjct: 191 G-KDVLILGGGDGGILCEIVKLKP-KMVTMVEIDQMVIDGCKKYMRKTCGDVLDNLKGDC 248
Query: 133 KRAKLIIGDGV----EFIKENSGFDVVIVD------STDPVGPAKMLFSREFYERAYEAL 182
+++I D + + KE FD VI D ST P + F R + + + L
Sbjct: 249 --YQVLIEDCIPVLKRYAKEGREFDYVINDLTAVPISTSPEEDSTWEFLRLILDLSMKVL 306
Query: 183 NDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI---GYASPWAF 232
G Y TQ V L T+ L E + R++ V +S ++ Y W F
Sbjct: 307 KQDGKYFTQGNCVNL-TEALSLYEEQLGRLYCPV-EFSKEIVCVPSYLELWVF 357
>gb|AAD08634.1| (U53331) spermidine aminopropyltransferase [Homo sapiens]
Length = 349
Score = 74.5 bits (180), Expect = 1e-12
Identities = 65/233 (27%), Positives = 114/233 (48%), Gaps = 20/233 (8%)
Query: 13 VAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHP 72
V + + E +++ S +Q I++ ++ FG +L L G V L E + +Y +++ +
Sbjct: 115 VEYDIDEVVYDEDSPYQNIKILHSKQFGNILILSGDVNLA-ESDLAYTRAIMGSGKEDYT 173
Query: 73 NPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGEC 132
+ VL++GGGDGG + E+++ + + MVEID+ VI+ K++ G + +KG+C
Sbjct: 174 G-KDVLILGGGDGGILCEIVKLKP-KMVTMVEIDQMVIDGCKKYMRKTCGDVLDNLKGDC 231
Query: 133 KRAKLIIGDGV----EFIKENSGFDVVIVD------STDPVGPAKMLFSREFYERAYEAL 182
+++I D + + KE FD VI D ST P + F R + + + L
Sbjct: 232 --YQVLIEDCIPVLKRYAKEGREFDYVINDLTAVPISTSPEEDSTWEFLRLILDLSMKVL 289
Query: 183 NDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI---GYASPWAF 232
G Y TQ V L T+ L E + R++ V +S ++ Y W F
Sbjct: 290 KQDGKYFTQGNCVNL-TEALSLYEEQLGRLYCPV-EFSKEIVCVPSYLELWVF 340
>gi|6678033 spermine synthase [Mus musculus] >gi|8134713|sp|P97355|SPSY_MOUSE
SPERMINE SYNTHASE (SPERMIDINE AMINOPROPYLTRANSFERASE)
(SPMSY) >gi|1707596|emb|CAA70573.1| (Y09419) spermine
synthase [Mus musculus] >gi|2625114|gb|AAB86631.1|
(AF031486) spermidine aminopropyltransferase [Mus
musculus]
Length = 366
Score = 72.6 bits (175), Expect = 5e-12
Identities = 64/233 (27%), Positives = 114/233 (48%), Gaps = 20/233 (8%)
Query: 13 VAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHP 72
V + + E +++ S +Q I++ ++ FG +L L G V L E + +Y +++ +
Sbjct: 132 VEYDIDEVVYDEDSPYQNIKILHSKQFGNILILSGDVNLA-ESDLAYTRAIMGSGKEDYT 190
Query: 73 NPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGEC 132
+ VL++GGGDGG + E+++ + + MVEID+ VI+ K++ G + ++G+C
Sbjct: 191 G-KDVLILGGGDGGILCEIVKLKP-KMVTMVEIDQMVIDGCKKYMRRTCGDVLDNLRGDC 248
Query: 133 KRAKLIIGDGVE----FIKENSGFDVVIVD------STDPVGPAKMLFSREFYERAYEAL 182
+++I D + + KE FD VI D ST P + F R + + + L
Sbjct: 249 --YQVLIEDCIPVLKMYAKEGREFDYVINDLTAVPISTSPEEDSTWDFLRLILDLSMKVL 306
Query: 183 NDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI---GYASPWAF 232
G Y TQ V L T+ L E + R++ V +S ++ Y W F
Sbjct: 307 KQDGKYFTQGNCVNL-TEALSLYEEQLGRLYCPV-EFSKEIVCVPSYLELWVF 357
>emb|CAA08902.1| (AJ009864) spermine synthase [Danio rerio]
>gi|4049415|emb|CAA08763.1| (AJ009633) unnamed protein
product [Danio rerio]
Length = 360
Score = 72.6 bits (175), Expect = 5e-12
Identities = 68/231 (29%), Positives = 111/231 (47%), Gaps = 20/231 (8%)
Query: 15 FKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHPNP 74
+ + E ++E S +Q I++ + FG +L L+G V L E + Y ++++ H
Sbjct: 131 YDIDEVVYEKDSAYQNIKILHSRQFGNMLILNGDVNL-AESDLPYTQAIMGSGK-EHYAG 188
Query: 75 RRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGECKR 134
+ VL++GGGDGG + E ++ + + MVEIDE VI+ K + G + +KG+C
Sbjct: 189 KEVLILGGGDGGILHEAVKLKP-KMITMVEIDELVIDGCRKHMRKTCGNVLDNLKGDC-- 245
Query: 135 AKLIIGDGV----EFIKENSGFDVVIVD------STDPVGPAKMLFSREFYERAYEALND 184
++++ D V +F ++ FD VI D ST P + F R + + L
Sbjct: 246 YEILVQDCVPVLKKFAEQGRTFDYVINDLTAVPISTAPEEDSTWEFLRLILDLSIRVLRP 305
Query: 185 PGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI---GYASPWAF 232
G Y TQ V L TD L + + R+ KV +S V+ Y W F
Sbjct: 306 GGKYFTQGNCVNL-TDALSEYEKLLGRLSCKV-DFSKEVVCVPSYMELWVF 354
>emb|CAA08903.1| (AJ009865) spermine synthase [Fugu rubripes]
Length = 359
Score = 69.1 bits (166), Expect = 6e-11
Identities = 59/233 (25%), Positives = 116/233 (49%), Gaps = 21/233 (9%)
Query: 13 VAFKVKEKIFEGHSKFQKIEVYETEGFGRLLALDGTVQLVTEGEESYHESLVHPVMLAHP 72
+ + + + ++E S +Q I++ ++ +G +L L+G + L + + +Y +++ ++
Sbjct: 129 IEYDIDQVLYEEDSAYQNIQILHSKQYGNILVLNGDINLA-DSDTAYTHAIMGSGKESYS 187
Query: 73 NPRRVLVIGGGDGGTIREVLRHEEVEEAIMVEIDEEVIEVAIKFIGIDGGLLDKMMKGEC 132
+ VL++GGGDGG + EV++ ++ + M+EID++VI+ + G + + G+C
Sbjct: 188 G-KEVLILGGGDGGILAEVVK-QKPKMITMLEIDQKVIDGCKTHMRKTCGDVLDNLTGDC 245
Query: 133 KRAKLIIGDGV----EFIKENSGFDVVIVD------STDPVGPAKMLFSREFYERAYEAL 182
++++ D V ++++E FD VI D ST+P + F R + + + L
Sbjct: 246 --YQILVEDCVPVLKKYVQEGRMFDYVINDLTAVPISTEP-EDSMWEFLRLILDLSIKVL 302
Query: 183 NDPGIYVTQAGSVYLFTDELVTAYENMSRVFDKVYAYSFPVI---GYASPWAF 232
+ G Y TQ V + TD L T YE +S V+ Y W F
Sbjct: 303 HPSGKYFTQGNGVNM-TDAL-TLYEEQLEKLSCPVGFSKEVVCVPSYLELWVF 353
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.142 0.416
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 107895329
Number of Sequences: 2977
Number of extensions: 4763523
Number of successful extensions: 11567
Number of sequences better than 1.0e-10: 71
Number of HSP's better than 0.0 without gapping: 57
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 11362
Number of HSP's gapped (non-prelim): 73
length of query: 279
length of database: 189,106,746
effective HSP length: 53
effective length of query: 226
effective length of database: 157,386,935
effective search space: 35569447310
effective search space used: 35569447310
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 164 (68.3 bits)