BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2236 (pri1) DE:DNA primase
(345 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75207 DNA primase PAB2236 - Pyrococcus abyssi (strain Orsa... 707 0.0
sp|O57934|PRIM_PYRHO PUTATIVE DNA PRIMASE >gi|7518519|pir||A7124... 608 e-173
dbj|BAB03293.1| (AB037376) DNA primase [Pyrococcus furiosus] 582 e-165
pir||T50696 DNA primase small chain homolog [imported] - thermop... 432 e-120
sp|O26685|PRIM_METTH PUTATIVE DNA PRIMASE >gi|7482226|pir||E6917... 233 2e-60
gi|11498348 DNA primase, putative [Archaeoglobus fulgidus] >gi|8... 109 5e-23
gb|AAG19774.1| (AE005062) DNA primase; Pri [Halobacterium sp. NR... 76 4e-13
>pir||C75207 DNA primase PAB2236 - Pyrococcus abyssi (strain Orsay)
>gi|5457615|emb|CAB49106.1| (AJ248283) DNA primase
[Pyrococcus abyssi]
Length = 345
Score = 707 bits (1804), Expect = 0.0
Identities = 345/345 (100%), Positives = 345/345 (100%)
Query: 1 MLLREVTKEERKEFYSNEWNAKQIPDFILQNLDKREFGFDHTGEGPSDRKNSYTDVRDLE 60
MLLREVTKEERKEFYSNEWNAKQIPDFILQNLDKREFGFDHTGEGPSDRKNSYTDVRDLE
Sbjct: 1 MLLREVTKEERKEFYSNEWNAKQIPDFILQNLDKREFGFDHTGEGPSDRKNSYTDVRDLE 60
Query: 61 DYIKATAPYAVYSSVAFYEKPQEMEGWLGAELVFDIDAKDLPLRRCNHEPGKVCPICLND 120
DYIKATAPYAVYSSVAFYEKPQEMEGWLGAELVFDIDAKDLPLRRCNHEPGKVCPICLND
Sbjct: 61 DYIKATAPYAVYSSVAFYEKPQEMEGWLGAELVFDIDAKDLPLRRCNHEPGKVCPICLND 120
Query: 121 AKEIARDTLIVLKEELGFEDVHVVYSGRGYHIRVMDGWALSLDSKSRERILSFISASEIE 180
AKEIARDTLIVLKEELGFEDVHVVYSGRGYHIRVMDGWALSLDSKSRERILSFISASEIE
Sbjct: 121 AKEIARDTLIVLKEELGFEDVHVVYSGRGYHIRVMDGWALSLDSKSRERILSFISASEIE 180
Query: 181 DHSEFRKMLLERRGWFVLNHGYPRVFRLRFGYFILRVKVEHLINFGIRKNIAKRILDNKE 240
DHSEFRKMLLERRGWFVLNHGYPRVFRLRFGYFILRVKVEHLINFGIRKNIAKRILDNKE
Sbjct: 181 DHSEFRKMLLERRGWFVLNHGYPRVFRLRFGYFILRVKVEHLINFGIRKNIAKRILDNKE 240
Query: 241 TIYEEFVRKGILAAFPDGVGIESLAKLFALSTRFSKAYFDGRVTVDLKRILRLPSTLHSK 300
TIYEEFVRKGILAAFPDGVGIESLAKLFALSTRFSKAYFDGRVTVDLKRILRLPSTLHSK
Sbjct: 241 TIYEEFVRKGILAAFPDGVGIESLAKLFALSTRFSKAYFDGRVTVDLKRILRLPSTLHSK 300
Query: 301 VGLIAKYIGNNERDVMRFNPFKHAVPKFRRKEVKEEYKRFLEENF 345
VGLIAKYIGNNERDVMRFNPFKHAVPKFRRKEVKEEYKRFLEENF
Sbjct: 301 VGLIAKYIGNNERDVMRFNPFKHAVPKFRRKEVKEEYKRFLEENF 345
>sp|O57934|PRIM_PYRHO PUTATIVE DNA PRIMASE >gi|7518519|pir||A71242 hypothetical protein
PH0195 - Pyrococcus horikoshii
>gi|3256581|dbj|BAA29264.1| (AP000001) 346aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 346
Score = 608 bits (1552), Expect = e-173
Identities = 287/342 (83%), Positives = 314/342 (90%)
Query: 1 MLLREVTKEERKEFYSNEWNAKQIPDFILQNLDKREFGFDHTGEGPSDRKNSYTDVRDLE 60
MLLREVT+EERK FY+NEW K IPDFI++ L+ REFGFDH+GEGPSDRKN YTD+RDLE
Sbjct: 1 MLLREVTREERKNFYTNEWKVKDIPDFIVKTLELREFGFDHSGEGPSDRKNQYTDIRDLE 60
Query: 61 DYIKATAPYAVYSSVAFYEKPQEMEGWLGAELVFDIDAKDLPLRRCNHEPGKVCPICLND 120
DYI+ATAPYAVYSSVA YEKPQEMEGWLG ELVFDIDAKDLPLRRC HEPG VCPICLND
Sbjct: 61 DYIRATAPYAVYSSVALYEKPQEMEGWLGTELVFDIDAKDLPLRRCEHEPGTVCPICLND 120
Query: 121 AKEIARDTLIVLKEELGFEDVHVVYSGRGYHIRVMDGWALSLDSKSRERILSFISASEIE 180
AKEI RDT+I+L+EELGF D+H++YSGRGYHIRV+D WAL LDSKSRERILSF+SASEIE
Sbjct: 121 AKEIVRDTVIILREELGFNDIHIIYSGRGYHIRVLDEWALKLDSKSRERILSFVSASEIE 180
Query: 181 DHSEFRKMLLERRGWFVLNHGYPRVFRLRFGYFILRVKVEHLINFGIRKNIAKRILDNKE 240
D EFRK+LL +RGWFVLNHGYPR FRLRFGYFILR+K+ HLIN GIRK+IAK IL +KE
Sbjct: 181 DVEEFRKLLLNKRGWFVLNHGYPRAFRLRFGYFILRIKLPHLINAGIRKSIAKSILKSKE 240
Query: 241 TIYEEFVRKGILAAFPDGVGIESLAKLFALSTRFSKAYFDGRVTVDLKRILRLPSTLHSK 300
IYEEFVRK ILAAFP GVGIESLAKLFALSTRFSK+YFDGRVTVDLKRILRLPSTLHSK
Sbjct: 241 EIYEEFVRKAILAAFPQGVGIESLAKLFALSTRFSKSYFDGRVTVDLKRILRLPSTLHSK 300
Query: 301 VGLIAKYIGNNERDVMRFNPFKHAVPKFRRKEVKEEYKRFLE 342
VGLIAKY+G NERDVMRFNPFKHAVPKFR++EVK EYK+FLE
Sbjct: 301 VGLIAKYVGTNERDVMRFNPFKHAVPKFRKEEVKVEYKKFLE 342
>dbj|BAB03293.1| (AB037376) DNA primase [Pyrococcus furiosus]
Length = 347
Score = 582 bits (1484), Expect = e-165
Identities = 272/342 (79%), Positives = 311/342 (90%)
Query: 1 MLLREVTKEERKEFYSNEWNAKQIPDFILQNLDKREFGFDHTGEGPSDRKNSYTDVRDLE 60
ML+REVTKEER EFYS EW+AK+IP FI+ L+ REFGFDH GEGPSDRKN Y+D+RDLE
Sbjct: 1 MLMREVTKEERSEFYSKEWSAKKIPKFIVDTLESREFGFDHNGEGPSDRKNQYSDIRDLE 60
Query: 61 DYIKATAPYAVYSSVAFYEKPQEMEGWLGAELVFDIDAKDLPLRRCNHEPGKVCPICLND 120
DYI+AT+PYAVYSSVAFYE P+EMEGW GAELVFDIDAKDLPL+RCNHEPG VCPICL D
Sbjct: 61 DYIRATSPYAVYSSVAFYENPREMEGWRGAELVFDIDAKDLPLKRCNHEPGTVCPICLED 120
Query: 121 AKEIARDTLIVLKEELGFEDVHVVYSGRGYHIRVMDGWALSLDSKSRERILSFISASEIE 180
AKE+A+DTLI+L+EELGFE++HVVYSGRGYHIR++D WAL LDSKSRERIL+FISASEIE
Sbjct: 121 AKELAKDTLIILREELGFENIHVVYSGRGYHIRILDEWALQLDSKSRERILAFISASEIE 180
Query: 181 DHSEFRKMLLERRGWFVLNHGYPRVFRLRFGYFILRVKVEHLINFGIRKNIAKRILDNKE 240
+ EFR+ LLE+RGWFVL HGYPRVFRLR GYFILRV V HL++ GIR+NIAK+ILD+KE
Sbjct: 181 NVEEFRRFLLEKRGWFVLKHGYPRVFRLRLGYFILRVNVPHLLSIGIRRNIAKKILDHKE 240
Query: 241 TIYEEFVRKGILAAFPDGVGIESLAKLFALSTRFSKAYFDGRVTVDLKRILRLPSTLHSK 300
IYE FVRK ILA+FP+GVGIES+AKLFALSTRFSKAYFDGRVTVD+KRILRLPSTLHSK
Sbjct: 241 EIYEGFVRKAILASFPEGVGIESMAKLFALSTRFSKAYFDGRVTVDIKRILRLPSTLHSK 300
Query: 301 VGLIAKYIGNNERDVMRFNPFKHAVPKFRRKEVKEEYKRFLE 342
VGLIA Y+G ER+VM+FNPF+HAVPKFR+KEV+E YK + E
Sbjct: 301 VGLIATYVGTKEREVMKFNPFRHAVPKFRKKEVREAYKLWRE 342
>pir||T50696 DNA primase small chain homolog [imported] - thermophilic bacterium
'Bonch-Osmolovskaya' >gi|3342819|gb|AAC27713.1|
(AF076685) primase small subunit homolog [thermophilic
archaeon 'Bonch-Osmolovskaya']
Length = 357
Score = 432 bits (1100), Expect = e-120
Identities = 217/355 (61%), Positives = 269/355 (75%), Gaps = 17/355 (4%)
Query: 2 LLREVTKEERKEFYSNEWNAKQIPDFILQNLDKREFGFDHTGEGPSDRKNSYTDVRDLED 61
L RE TKEER ++Y EW+AK++P+FI++ L+ REFGFDHTGEGPSDRKN + DVRDLED
Sbjct: 4 LFREATKEERAKYYRGEWSAKRLPEFIVETLENREFGFDHTGEGPSDRKNVFLDVRDLED 63
Query: 62 YIKATAPYAVYSSVAFYEKPQEMEGWLGAELVFDIDAKDLPLRRCN--HEPGKVCPICLN 119
Y+KATAPYAVYSSVA YE+P+EMEGWLGAELVFDIDAKDLPLRRC HE GKVCPICL
Sbjct: 64 YMKATAPYAVYSSVALYEEPREMEGWLGAELVFDIDAKDLPLRRCQHIHEHGKVCPICLE 123
Query: 120 DAKEIARDTLIVLKEELGFEDVHVVYSGRGYHIRVMDGWALSLDSKSRERILSFISASEI 179
DAKE+ARDTLIVLKE+ GFE VHV+YSGRGYHIRV+D W D K+RE+IL+++SA+E
Sbjct: 124 DAKELARDTLIVLKEDFGFESVHVIYSGRGYHIRVLDDWRWPSDGKAREKILAYVSAAE- 182
Query: 180 EDHSEFRKMLLERRGWFVLNHGYPRVFRLRFGYFILRVKVEHLINFGIRKNIAKRILDNK 239
+++K + + +L+ GY RVFR+RFGYF+ R+ HL NFG+ + K +L+N
Sbjct: 183 --ELDYKKDVQSNK--IMLSSGYYRVFRIRFGYFLKRISENHLRNFGLSEKQIKDVLENI 238
Query: 240 ETIYEEFV--RKGILAAFPD--------GVGIESLAKLFALSTRFSKAYFDGRVTVDLKR 289
+ IY +FV K AFP G+ L L S+ FSKAYFDGRVTVD+KR
Sbjct: 239 DKIYNDFVLNAKSPNIAFPSSLRRPPKKGLHKSGLMILLNSSSTFSKAYFDGRVTVDVKR 298
Query: 290 ILRLPSTLHSKVGLIAKYIGNNERDVMRFNPFKHAVPKFRRKEVKEEYKRFLEEN 344
ILRLPS+LHSKVG I YIG +E + RFNPF+ AVP+FRR+EV+E Y+ +LEE+
Sbjct: 299 ILRLPSSLHSKVGFITTYIGPDEGKLERFNPFEDAVPRFRREEVREAYRLWLEEH 353
>sp|O26685|PRIM_METTH PUTATIVE DNA PRIMASE >gi|7482226|pir||E69177 conserved hypothetical
protein MTH585 - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2621663|gb|AAB85091.1| (AE000840)
DNA primase, small subunit [Methanobacterium
thermoautotrophicum]
Length = 323
Score = 233 bits (589), Expect = 2e-60
Identities = 131/332 (39%), Positives = 188/332 (56%), Gaps = 22/332 (6%)
Query: 2 LLREVTKEERKEFYSNEWNAKQIPDFILQNLDKREFGFDHTGEGPSDRKNSYTDVRDLED 61
+ T ERK +Y EWN + +PDFI NL REFGFDH G GPSDR + D R L
Sbjct: 4 MFEPATPLERKRYYREEWNVRDLPDFIADNLHMREFGFDHNGRGPSDRYRVFRDERSLAR 63
Query: 62 YIKATAPYAVYSSVAFYEKPQEMEGWLGAELVFDIDAKDLPLRRCNHEPGKVCPICLNDA 121
+++ P+A YSSVAFY +P + +GW +EL+FD+DAKDLP+R C E VCP CL++A
Sbjct: 64 FMRVRYPFAAYSSVAFYREPWKRKGWQRSELIFDVDAKDLPVRSC--ECDGVCPTCLDEA 121
Query: 122 KEIARDTLIVLKEELGFEDVHVVYSGRGYHIRVMDGWALSLDSKSRERILSFISASEIED 181
+E+ + LK +LG D+HV+YSGRGYH+RV+D + L S+ R IL + + +
Sbjct: 122 RELVLMMVDTLKGDLGLGDIHVIYSGRGYHVRVLDPEVMELGSEVRAEILRYTAGAR--- 178
Query: 182 HSEFRKMLLERRGWFVLNH-----GYPRVFRLRFGYFILRVKVEHLINFGIRKNIAKRIL 236
E R+ + + + H GY RVF R + +L ++ + I + K +
Sbjct: 179 --EPRRKFTDGVSSYEMEHFSIPLGYHRVFTERARHVLLHLRGDEDIE-DVTARTVKTAV 235
Query: 237 DNKETIYEEFVRKGILAAFPDGVGIESLAKLFALSTRFSKAYFDGRVTVDLKRILRLPST 296
N+ I E+ R G F +G +L ++ + D +VT+DLKRILRLP++
Sbjct: 236 RNRNLILED--RWG---EFRSVIGPRVYPRLVKGISKINMRMLDAKVTIDLKRILRLPTS 290
Query: 297 LHSKVGLIAKYIGNNERDVMRFNPFKHAVPKF 328
LHSKV +I + N E F+P K AVP+F
Sbjct: 291 LHSKVSMICMEVKNPE----TFDPLKSAVPRF 318
>gi|11498348 DNA primase, putative [Archaeoglobus fulgidus]
>gi|8928289|sp|O29516|PRIM_ARCFU PUTATIVE DNA PRIMASE
>gi|7483287|pir||F69342 DNA primase homolog -
Archaeoglobus fulgidus >gi|2649873|gb|AAB90500.1|
(AE001054) DNA primase, putative [Archaeoglobus
fulgidus]
Length = 360
Score = 109 bits (270), Expect = 5e-23
Identities = 99/333 (29%), Positives = 152/333 (44%), Gaps = 57/333 (17%)
Query: 1 MLLREVTKEERKEFYSNEWNAKQIPDFILQNLDKREFGFDHTGEGPS---DRKNSYTDVR 57
ML + K++ +E+YS N ++P + REF F P R S+
Sbjct: 26 MLTKLFLKKKFEEYYSK--NEVELP----RKFKNREFAFVPLELLPDFVMHRHISFRSET 79
Query: 58 DLEDYIKATAPYAVYSSVAFYEKP----QEMEGWLGAELVFDIDAKDLPLRRCNHEPGKV 113
D YI + P +Y S A+YE+P E +GWLGA+L+FDIDA LP++ + E
Sbjct: 80 DFRAYILSNVPAHIYFSSAYYERPAEDKMENKGWLGADLIFDIDADHLPVKAQSFEK--- 136
Query: 114 CPICLNDAKEIARDTLIVLKEELGFEDVHVVYS-GRGYHIRVMDGWALSLDSKSRERILS 172
L AK + VL+ + G D+ + +S GRGYH+ V D LSL S R I+
Sbjct: 137 ---ALEMAKREIKKLTAVLRADFGIRDMKIYFSGGRGYHVHVHDEEFLSLGSAERREIVD 193
Query: 173 FISASEIEDHSEFRKMLLERRGWFVLNHGYPRVFRLRFGYFILRVKVEHLINFGIRKNIA 232
++ + K+++E R F ++ RV LR K+E + +
Sbjct: 194 YLRLNS-------PKIVVEDR--FANSNAAKRVLN------YLRKKLEE------DERLT 232
Query: 233 KRILDNKETIYEEFVRKGILAAFPDGVGIESLAKLFALSTRFSKAYFDGRVTVDLKRILR 292
++ + +E + K ++ A +E ALS Y D VT D+KR++R
Sbjct: 233 SKLKIKPADLKKEKLTKKVIRA------VEKF-DYSALS-----IYIDAPVTADVKRLIR 280
Query: 293 LPSTLHSKVGLIAKYIGNNERDVMRFNPFKHAV 325
LP +LH K GL + D+ FNP K A+
Sbjct: 281 LPGSLHGKTGLRVTEV----EDIESFNPLKDAL 309
>gb|AAG19774.1| (AE005062) DNA primase; Pri [Halobacterium sp. NRC-1]
Length = 424
Score = 76.5 bits (185), Expect = 4e-13
Identities = 80/319 (25%), Positives = 131/319 (40%), Gaps = 32/319 (10%)
Query: 33 DKREFGFDHTGEGPSD---RKNSYTDVRDLEDYIKATAPYAVYSSVAFYEKP----QEME 85
D RE+G+ GP R S D+ + +++ P VY S Y+ P + +
Sbjct: 62 DDREWGYIPWTAGPDTTMVRHKSTLDLGSMTSFLERKCPRHVYFSAGTYDAPGAATMDEK 121
Query: 86 GWLGAELVFDIDAKDLPLRRCNHEPGKVCPICLNDAKEIARDTLIVLKEELGFEDVHVVY 145
W G++LVFD+DA LP + L+ K+ L L+ + GFE++ V +
Sbjct: 122 HWQGSDLVFDLDADHLPRVTLGEDSYAEM---LSKCKDALLRLLDFLERDFGFEELTVTF 178
Query: 146 SG-RGYHIRVMDGWALSLDSKSRERILSFISASEIEDHSEFRKMLLERRG--------WF 196
SG RGYH+ V D L S+ R I+ ++ +++ + + RG
Sbjct: 179 SGGRGYHVHVRDAGVYELGSEERREIVDYVRGNDLALETLVEAEPVGGRGLENPTEKRML 238
Query: 197 VLNHGYPRVFRLRFGYFILRV-------KVEHLINFGIRKNIAKRILDNKETIYEEFVRK 249
+ G+ R R F + V L F + R + N V++
Sbjct: 239 PADGGWGRRVTTRLEAFADDLIERGEDDAVATLTEFDGVGERSARAIYNVVADNTTAVKR 298
Query: 250 GILAAFPDGVGIESLAKLFALST-RFSKAYFDGRVTVDLKRILRLPSTLHSKVGLIAKYI 308
G + P ++A+ + T +A D VT D R++RLP +LH GL + +
Sbjct: 299 GNVDVHP---AFLTVARRYIDETVAAEQAPIDEPVTTDTNRLIRLPGSLHGGSGLEVQRL 355
Query: 309 GNNERDVMRFNPFKHAVPK 327
D F+P AVP+
Sbjct: 356 DRAALD--DFDPLVDAVPE 372
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.323 0.142 0.420
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 130099547
Number of Sequences: 2977
Number of extensions: 5557979
Number of successful extensions: 12276
Number of sequences better than 1.0e-10: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 12259
Number of HSP's gapped (non-prelim): 9
length of query: 345
length of database: 189,106,746
effective HSP length: 53
effective length of query: 292
effective length of database: 157,386,935
effective search space: 45956985020
effective search space used: 45956985020
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 165 (68.7 bits)