BLASTP 2.0.10 [Aug-26-1999]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= PAB2244 (nadE) DE:NH(3)-dependent NAD+ synthetase (nadE)
         (257 letters)

Database: ./suso.pep; /banques/blast2/nr.pep
           598,487 sequences; 189,106,746 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||B75205 nh(3)-dependent nad+ synthetase (nade) PAB2244 - Pyr...   515  e-145
sp|O57921|NADE_PYRHO PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE ...   431  e-120
pir||E69068 NH(3)-dependent NAD+ synthetase - Methanobacterium t...   243  2e-63
sp|O27554|NADE_METTH PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE       243  2e-63
gi|11498605 NH(3)-dependent NAD+ synthetase (nadE) [Archaeoglobu...   228  4e-59
sp|Q58747|NADE_METJA PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE ...   223  1e-57
emb|CAC12028.1| (AL445065) NAD(+) synthase related protein [Ther...   203  1e-51
sp|Q9YAI1|NADE_AERPE PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE       195  5e-49
pir||H72497 probable NH(3)-dependent NAD(+) synthetase APE1958 -...   187  1e-46
gb|AAK42347.1| NH(3+) dependent NAD(+) synthetase (nadE) [Sulfol...   182  3e-45
gb|AAG20193.1| (AE005096) NAD+ synthetase; NadE [Halobacterium s...   178  7e-44
sp|P47623|NADE_MYCGE PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE ...   166  2e-40
gb|AAF22217.1|AF140575_2 (AF140575) NH(3)-dependent NAD+ synthet...   164  7e-40
pir||B81353 probable NAD+ synthase (glutamine-hydrolyzing) (EC 6...   162  5e-39
sp|P75216|NADE_MYCPN PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE ...   156  2e-37
sp|O67091|NADE_AQUAE PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHET...   154  8e-37
pir||S48586 hypothetical protein - Mycoplasma capricolum (SGC3) ...   151  9e-36
sp|Q9WZB3|NAE1_THEMA NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|74489...   150  2e-35
sp|Q9X0Y0|NAE2_THEMA PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHET...   137  1e-31
sp|O25096|NADE_HELPY NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|74489...   128  8e-29
sp|P08164|NADE_BACSU NH(3)-DEPENDENT NAD(+) SYNTHETASE (SPORE OU...   122  4e-27
pdb|2NSY|A Chain A, Crystal Structure Of Nh3-Dependent Nad+ Synt...   122  4e-27
sp|P74292|NADE_SYNY3 PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHET...   122  6e-27
emb|CAB58266.1| (AL121849) NH(3)-dependent NAD(+)synthetase (EC ...   121  1e-26
sp|Q9ZMB0|NADE_HELPJ NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|74489...   120  2e-26
pir||T51756 NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) [...   120  2e-26
pir||C82617 NH3-dependent NAD synthetase XF1961 [imported] - Xyl...   120  2e-26
pir||H82488 NH(3)-dependent NAD(+) synthetase VCA0207 [imported]...   119  3e-26
pir||F83030 NH3-dependent NAD synthetase PA4920 [imported] - Pse...   119  4e-26
pir||A75617 NH3-dependent NAD+ synthase - Deinococcus radioduran...   117  1e-25
pir||C81850 NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) N...   117  1e-25
sp|Q03638|NADE_RHOCA GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(...   114  1e-24
pir||A81090 NH(3)-dependent NAD+ synthetase NadE, probable NMB13...   112  4e-24
sp|P18843|NADE_ECOLI NH(3)-DEPENDENT NAD(+) SYNTHETASE (NITROGEN...   109  3e-23
pir||A26928 nitrogen-regulatory protein - Escherichia coli >gi|1...   104  1e-21
dbj|BAB06004.1| (AP001515) NH3-dependent NAD synthetase [Bacillu...   102  3e-21
sp|P57271|NADE_BUCAI NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|10038...   100  2e-20
pir||E82888 NH(3)-dependent NAD(+) synthetase UU460 [imported] -...    95  7e-19
gb|AAG27700.1|AF299296_3 (AF299296) NH(3)-dependent NAD(+)synthe...    90  2e-17
pir||A70165 NH(3)-dependent NAD+ synthetase homolog - Lyme disea...    75  7e-13

>pir||B75205 nh(3)-dependent nad+ synthetase (nade) PAB2244 - Pyrococcus abyssi
           (strain Orsay) >gi|5457598|emb|CAB49089.1| (AJ248283)
           NH(3)-dependent NAD+ synthetase (nadE) [Pyrococcus
           abyssi]
           Length = 257
           
 Score =  515 bits (1312), Expect = e-145
 Identities = 257/257 (100%), Positives = 257/257 (100%)

Query: 1   MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
           MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE
Sbjct: 1   MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60

Query: 61  NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHAN 120
           NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHAN
Sbjct: 61  NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHAN 120

Query: 121 SMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPT 180
           SMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPT
Sbjct: 121 SMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPT 180

Query: 181 AGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEH 240
           AGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEH
Sbjct: 181 AGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEH 240

Query: 241 KRRLPIGPSFDDLIAGP 257
           KRRLPIGPSFDDLIAGP
Sbjct: 241 KRRLPIGPSFDDLIAGP 257


>sp|O57921|NADE_PYRHO PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|7448970|pir||D71240
           probable NH(3)-dependent NAD(+) synthetase - Pyrococcus
           horikoshii >gi|3256568|dbj|BAA29251.1| (AP000001) 257aa
           long hypothetical NH(3)-dependent NAD(+) synthetase
           [Pyrococcus horikoshii]
           Length = 257
           
 Score =  431 bits (1097), Expect = e-120
 Identities = 205/257 (79%), Positives = 235/257 (90%)

Query: 1   MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
           MR LDY+K IERI++FIREK +NGVV+GISGGVDSATV YLATKALG+EKVLGLIMPY+E
Sbjct: 1   MRILDYDKVIERILEFIREKGNNGVVIGISGGVDSATVAYLATKALGKEKVLGLIMPYFE 60

Query: 61  NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHAN 120
           N+DVEDAKLV E LGI Y+VINI+PIVD+ V  + + LD+K LGN+M+RTRM++LYAHAN
Sbjct: 61  NKDVEDAKLVAEKLGIGYKVINIKPIVDSFVENLELNLDRKGLGNIMSRTRMIMLYAHAN 120

Query: 121 SMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPT 180
           S+ R+VLGTSNRSEFLTGYFTKWGDGASDYAP+INLYKTEVWEIAKRIGVPERIVKKKP+
Sbjct: 121 SLGRIVLGTSNRSEFLTGYFTKWGDGASDYAPIINLYKTEVWEIAKRIGVPERIVKKKPS 180

Query: 181 AGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEH 240
           AGLWEGQTDEDELGISY LLDEILWR++DLK+ KE+IA+DLGIP+  VE VE L+K SEH
Sbjct: 181 AGLWEGQTDEDELGISYNLLDEILWRMIDLKIGKEEIAKDLGIPLSLVERVEELIKKSEH 240

Query: 241 KRRLPIGPSFDDLIAGP 257
           KRRLPIGPSF+DLI GP
Sbjct: 241 KRRLPIGPSFEDLIVGP 257


>pir||E69068 NH(3)-dependent NAD+ synthetase - Methanobacterium
           thermoautotrophicum (strain Delta H)
           >gi|2622628|gb|AAB85985.1| (AE000911) NH(3)-dependent
           NAD+ synthetase [Methanobacterium thermoautotrophicum]
           Length = 274
           
 Score =  243 bits (613), Expect = 2e-63
 Identities = 125/247 (50%), Positives = 169/247 (67%), Gaps = 6/247 (2%)

Query: 8   KAIERIIDFIREKCS----NGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYEN-- 61
           + +  I DFIR+K +    +GVV+G+SGGVDS+TV YLA  ALG ++VLGLIMP      
Sbjct: 18  EVVSVIEDFIRQKVAESGASGVVLGLSGGVDSSTVAYLAVNALGPDRVLGLIMPSSTTPR 77

Query: 62  QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANS 121
            D+  A+ V + LGIE   I+I PI++++        ++ +L N+  R RM+ILY HANS
Sbjct: 78  DDLRHARTVADELGIESETIDIDPIIESLTGLCSHNANELALANLKPRARMVILYYHANS 137

Query: 122 MNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTA 181
           +NR+V GT NR+E L GYFTK+GDG  D  P+  LYK +V E+A R+GVP  I+KK PTA
Sbjct: 138 LNRLVAGTGNRTELLLGYFTKYGDGGVDMLPIGGLYKGQVRELAGRLGVPPEIIKKPPTA 197

Query: 182 GLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHK 241
           GLW GQTDE+ELG+ Y LLDE+L  LVD K+  E++A  L +P  +VE + ++VKGSEHK
Sbjct: 198 GLWHGQTDEEELGMKYDLLDELLCLLVDRKLPVEEVASTLSLPPSEVERIASMVKGSEHK 257

Query: 242 RRLPIGP 248
            + P  P
Sbjct: 258 LKPPEVP 264


>sp|O27554|NADE_METTH PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE
           Length = 266
           
 Score =  243 bits (613), Expect = 2e-63
 Identities = 125/247 (50%), Positives = 169/247 (67%), Gaps = 6/247 (2%)

Query: 8   KAIERIIDFIREKCS----NGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYEN-- 61
           + +  I DFIR+K +    +GVV+G+SGGVDS+TV YLA  ALG ++VLGLIMP      
Sbjct: 10  EVVSVIEDFIRQKVAESGASGVVLGLSGGVDSSTVAYLAVNALGPDRVLGLIMPSSTTPR 69

Query: 62  QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANS 121
            D+  A+ V + LGIE   I+I PI++++        ++ +L N+  R RM+ILY HANS
Sbjct: 70  DDLRHARTVADELGIESETIDIDPIIESLTGLCSHNANELALANLKPRARMVILYYHANS 129

Query: 122 MNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTA 181
           +NR+V GT NR+E L GYFTK+GDG  D  P+  LYK +V E+A R+GVP  I+KK PTA
Sbjct: 130 LNRLVAGTGNRTELLLGYFTKYGDGGVDMLPIGGLYKGQVRELAGRLGVPPEIIKKPPTA 189

Query: 182 GLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHK 241
           GLW GQTDE+ELG+ Y LLDE+L  LVD K+  E++A  L +P  +VE + ++VKGSEHK
Sbjct: 190 GLWHGQTDEEELGMKYDLLDELLCLLVDRKLPVEEVASTLSLPPSEVERIASMVKGSEHK 249

Query: 242 RRLPIGP 248
            + P  P
Sbjct: 250 LKPPEVP 256


>gi|11498605 NH(3)-dependent NAD+ synthetase (nadE) [Archaeoglobus fulgidus]
           >gi|6093466|sp|O29262|NADE_ARCFU PROBABLE
           NH(3)-DEPENDENT NAD(+) SYNTHETASE
           >gi|7448971|pir||H69374 NH(3)-dependent NAD+ synthetase
           (nadE) homolog - Archaeoglobus fulgidus
           >gi|2649596|gb|AAB90242.1| (AE001035) NH(3)-dependent
           NAD+ synthetase (nadE) [Archaeoglobus fulgidus]
           Length = 247
           
 Score =  228 bits (576), Expect = 4e-59
 Identities = 130/258 (50%), Positives = 170/258 (65%), Gaps = 18/258 (6%)

Query: 4   LDYEKAIERIIDFIR----EKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP-- 57
           +++EK +ERI DFIR       S GVV+G+SGGVDSATV YL  +ALG E+V  LIMP  
Sbjct: 1   MNFEKVVERICDFIRGVVSSSGSTGVVLGLSGGVDSATVAYLCVRALGSERVFALIMPET 60

Query: 58  -YYENQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILY 116
                QDVEDA  V ESLG+EY++I I  IV     + G E  K +  N+  R RM++ Y
Sbjct: 61  GVTPEQDVEDAINVAESLGMEYKLIEINDIVRVFKEKAG-EGSKIAEANLKPRIRMVLNY 119

Query: 117 AHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVK 176
            HANSMNR+V GT N+SE + GYFTK+GDG  D+ P+ +LYKTEV+++A  +GVP RI++
Sbjct: 120 YHANSMNRLVAGTGNKSELMVGYFTKYGDGGVDFLPIGDLYKTEVFQLAAYLGVPRRIIE 179

Query: 177 KKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVK 236
           KKP+A LW GQTDE+E+GISY  LDEIL +L+    EK +  +D     EK   V  +V+
Sbjct: 180 KKPSARLWPGQTDEEEMGISYAELDEIL-KLI----EKGERRDD-----EKFRRVVQMVE 229

Query: 237 GSEHKRRLPIGPSFDDLI 254
            S HKR +P      DL+
Sbjct: 230 RSRHKREMPPVARVRDLL 247


>sp|Q58747|NADE_METJA PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|2129159|pir||G64468
           NH(3)-dependent NAD+ synthetase - Methanococcus
           jannaschii >gi|1591995|gb|AAB99363.1| (U67575)
           NH(3)-dependent NAD+ synthetase (nadE) [Methanococcus
           jannaschii]
           Length = 259
           
 Score =  223 bits (563), Expect = 1e-57
 Identities = 125/251 (49%), Positives = 168/251 (66%), Gaps = 10/251 (3%)

Query: 7   EKAIERIIDFIREKC----SNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYEN- 61
           E+ + +I  FIREK     +NGVVVG+SGG+DS+   YL  KALG++KVLGLIMP     
Sbjct: 8   EEIVNKITKFIREKVEEANANGVVVGLSGGIDSSVTAYLCVKALGKDKVLGLIMPEKNTN 67

Query: 62  -QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGI---ELDKKSLGNVMARTRMMILYA 117
            +DVE AK+V E+LGI+Y + +I  I+    +   +   E DK + GN+ AR RM ILY 
Sbjct: 68  PKDVEHAKMVAENLGIKYIISDITDILKAFGAGGYVPTREFDKIADGNLKARIRMCILYY 127

Query: 118 HANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKK 177
            AN  N +V GTSN+SE   GY TK GD A D  P+ NL+KTEV ++AK IGVP+ I++K
Sbjct: 128 FANKYNLLVAGTSNKSEIYVGYGTKHGDIACDIRPIGNLFKTEVKKLAKYIGVPKEIIEK 187

Query: 178 KPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKG 237
            P+AGLWEGQTDE+EL I Y+ LD IL +L +     E+I ++  IP+E + +V +L+K 
Sbjct: 188 PPSAGLWEGQTDEEELDIKYETLDTIL-KLYEKGKTPEEIHKETNIPLETINYVFDLIKK 246

Query: 238 SEHKRRLPIGP 248
           +EHKR LP  P
Sbjct: 247 NEHKRTLPPTP 257


>emb|CAC12028.1| (AL445065) NAD(+) synthase related protein [Thermoplasma
           acidophilum]
           Length = 241
           
 Score =  203 bits (512), Expect = 1e-51
 Identities = 107/244 (43%), Positives = 160/244 (64%), Gaps = 13/244 (5%)

Query: 5   DYEKAIERIIDFIREKCSN-GVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ- 62
           DY + IERI +F+R+       V+GISGG+DS+    L T+++ +E+++ + MP    Q 
Sbjct: 3   DYMQEIERISEFLRKVLQGKNAVIGISGGIDSSVTLALLTRSIPKERIIPVFMPDRNTQQ 62

Query: 63  -DVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANS 121
            D +D + + + LG+ YR + I P+VD+ VS +    D+ ++GN+ +RTRM+ILY  AN+
Sbjct: 63  ADYDDVQKLTQKLGLTYREVRIDPMVDSFVSTLAAT-DRAAIGNIKSRTRMIILYYFANT 121

Query: 122 MNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTA 181
              MV+GT+NR+E L GYFTK+GDG  D  P+ +LYK +V+E+AK + +PE I+KKKPTA
Sbjct: 122 NGGMVVGTTNRTELLLGYFTKYGDGGCDVEPIEHLYKRDVYELAKILDIPESIMKKKPTA 181

Query: 182 GLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHK 241
           GLW GQTDEDE+G+SY  +DEIL  + D     +K         +K+  + +L   S+HK
Sbjct: 182 GLWAGQTDEDEIGMSYAQMDEILSDVFDKGTYNDK------PEYKKIMEMHSL---SDHK 232

Query: 242 RRLP 245
           R LP
Sbjct: 233 RNLP 236


>sp|Q9YAI1|NADE_AERPE PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE
           Length = 280
           
 Score =  195 bits (490), Expect = 5e-49
 Identities = 113/261 (43%), Positives = 156/261 (59%), Gaps = 11/261 (4%)

Query: 4   LDYEKAIERIIDFIREKC----SNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYY 59
           +DY    + I  F+R+      ++G V+G+SGGVDS+    LA  A+G  +V  LIMP  
Sbjct: 9   IDYNGVRQAITQFLRKYLEASGASGYVLGVSGGVDSSLALALAVDAVGSGRVTALIMPDR 68

Query: 60  E---NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGI----ELDKKSLGNVMARTRM 112
           E    +DVEDA  +V S G+E+ VI+I PIV   +S + I    E D+  +GN+ AR R 
Sbjct: 69  EVTPERDVEDALRLVRSFGVEHAVIDISPIVMVYISALPIFEDEEKDRVPVGNLRARIRA 128

Query: 113 MILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPE 172
            ILY +AN + ++VLGT +RSE+L GYFTK+GD A D APL  LYK++V  +A+ IGVP 
Sbjct: 129 NILYYYANKLGKLVLGTGDRSEYLIGYFTKYGDAACDVAPLTVLYKSQVRRLAELIGVPR 188

Query: 173 RIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVE 232
            I  K  +  LW+G   E ELG+SY  +D IL+   DLK+  E+I    G+   KVE V 
Sbjct: 189 DIAYKPSSPRLWKGHEAEAELGLSYNEIDVILYSRFDLKIPWEEIPRATGLERAKVERVR 248

Query: 233 NLVKGSEHKRRLPIGPSFDDL 253
            L + S HKR  P  P   ++
Sbjct: 249 LLHEASSHKRSPPASPDLGEI 269


>pir||H72497 probable NH(3)-dependent NAD(+) synthetase APE1958 - Aeropyrum
           pernix (strain K1) >gi|5105656|dbj|BAA80968.1|
           (AP000063) 246aa long hypothetical NH(3)-dependent
           NAD(+) synthetase [Aeropyrum pernix]
           Length = 246
           
 Score =  187 bits (470), Expect = 1e-46
 Identities = 106/235 (45%), Positives = 144/235 (61%), Gaps = 7/235 (2%)

Query: 26  VVGISGGVDSATVTYLATKALGREKVLGLIMPYYE---NQDVEDAKLVVESLGIEYRVIN 82
           ++G+SGGVDS+    LA  A+G  +V  LIMP  E    +DVEDA  +V S G+E+ VI+
Sbjct: 1   MLGVSGGVDSSLALALAVDAVGSGRVTALIMPDREVTPERDVEDALRLVRSFGVEHAVID 60

Query: 83  IRPIVDTIVSEIGI----ELDKKSLGNVMARTRMMILYAHANSMNRMVLGTSNRSEFLTG 138
           I PIV   +S + I    E D+  +GN+ AR R  ILY +AN + ++VLGT +RSE+L G
Sbjct: 61  ISPIVMVYISALPIFEDEEKDRVPVGNLRARIRANILYYYANKLGKLVLGTGDRSEYLIG 120

Query: 139 YFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQTDEDELGISYK 198
           YFTK+GD A D APL  LYK++V  +A+ IGVP  I  K  +  LW+G   E ELG+SY 
Sbjct: 121 YFTKYGDAACDVAPLTVLYKSQVRRLAELIGVPRDIAYKPSSPRLWKGHEAEAELGLSYN 180

Query: 199 LLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRRLPIGPSFDDL 253
            +D IL+   DLK+  E+I    G+   KVE V  L + S HKR  P  P   ++
Sbjct: 181 EIDVILYSRFDLKIPWEEIPRATGLERAKVERVRLLHEASSHKRSPPASPDLGEI 235


>gb|AAK42347.1| NH(3+) dependent NAD(+) synthetase (nadE) [Sulfolobus solfataricus]
           Length = 304
           
 Score =  182 bits (458), Expect = 3e-45
 Identities = 101/238 (42%), Positives = 151/238 (63%), Gaps = 3/238 (1%)

Query: 10  IERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQDVEDAKL 69
           +ERI +++      G V+G+SGGVDSA    L  KA     +L +        D++D+  
Sbjct: 47  VERIREYLEFSNKKGGVIGVSGGVDSAVTATLLAKATDNFFILLMPSSSTPKIDLDDSFE 106

Query: 70  VVESLGIE--YRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANSMNRMVL 127
           +++ L  +  Y++INI  IV +  ++I  E +K  +GN+ AR RM+ILYA+A  ++ +V+
Sbjct: 107 MIKFLNAQNKYKLINIDEIVKSFSNKIETE-NKYIIGNIKARVRMIILYAYAQMLDYLVV 165

Query: 128 GTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQ 187
           GT ++SE L GYFTK+GDG  D  P+ +LYKT+V  + K +G+PERIV K  +  LWEGQ
Sbjct: 166 GTGDKSELLLGYFTKYGDGGVDVLPIGDLYKTQVRMLGKCLGLPERIVTKPSSPALWEGQ 225

Query: 188 TDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRRLP 245
           T E ELGI Y+ +D IL+   D    +++I + LGIPI+ V+ V+ LVK S+HKR  P
Sbjct: 226 TAEGELGIDYETIDSILYLRFDEMRSEDEIVKMLGIPIDIVKKVDRLVKISQHKRLPP 283


>gb|AAG20193.1| (AE005096) NAD+ synthetase; NadE [Halobacterium sp. NRC-1]
           Length = 268
           
 Score =  178 bits (446), Expect = 7e-44
 Identities = 104/245 (42%), Positives = 144/245 (58%), Gaps = 7/245 (2%)

Query: 11  ERIIDFIREKCSNG----VVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQDV 64
           +RI  FIR+  +       V+G+SGG+DS TV +L    LG + + GL+MP     +Q++
Sbjct: 24  DRIQSFIRDTVAAAGAERCVLGLSGGIDSTTVAHLTVDELGADALHGLVMPGAVSRDQNM 83

Query: 65  EDAKLVVESLGIEYRVINIRPIVDTIVSEIGIEL-DKKSLGNVMARTRMMILYAHANSMN 123
            DA+ V E LGIEY V+ I P V  +         D+ ++GN  ARTR +I Y  AN  +
Sbjct: 84  SDAERVAEDLGIEYDVVEIDPFVTQLTDVFPDAAGDEVAVGNARARTRAVINYFVANHGD 143

Query: 124 RMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGL 183
            +VLGT NR+E +TGY+TK+GD A D  P+ NLYK +V ++A+ +GVPE +V K PTA L
Sbjct: 144 GVVLGTGNRAEAMTGYYTKYGDQAVDCNPIGNLYKMQVRQLARDLGVPEDLVTKAPTAEL 203

Query: 184 WEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRR 243
           W  QTD  ELG+ Y  +D +L   VD  +     A  L I    VE V +L   S+HKR 
Sbjct: 204 WADQTDAGELGVDYDTIDAVLAVHVDGGLPASATATHLDIDPSVVETVRDLYGASKHKRA 263

Query: 244 LPIGP 248
           +P  P
Sbjct: 264 MPPAP 268


>sp|P47623|NADE_MYCGE PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|1361857|pir||D64242
           sporulation protein outB homolog - Mycoplasma genitalium
           >gi|3844972|gb|AAC71610.1| (U39720) NH(3)-dependent NAD+
           synthetase, putative [Mycoplasma genitalium]
           Length = 248
           
 Score =  166 bits (416), Expect = 2e-40
 Identities = 95/234 (40%), Positives = 139/234 (58%), Gaps = 7/234 (2%)

Query: 4   LDYEKAIER-IIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ 62
           + Y K ++  + D++++  + GV+ G+SGG+DSA V  +A +  G E  L LIM    ++
Sbjct: 5   IKYLKELQNWLFDYVKKSKAKGVIFGLSGGIDSAVVAAIAKETFGFENHLALIMHINNSK 64

Query: 63  -DVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSL--GNVMARTRMMILYAHA 119
            D +    +V+ +      I +    + +V  +GI+  K  L  GN+ AR RM+ LYA+A
Sbjct: 65  LDFQATSELVKKMQFNSINIELEESFNLLVKTLGIDPKKDFLTAGNIKARLRMITLYAYA 124

Query: 120 NSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKP 179
              N +VLGT N  E+  GYFTKWGDGA D APL  L K +V+++AK   +PE ++ + P
Sbjct: 125 QKHNFLVLGTGNFVEYTLGYFTKWGDGACDIAPLAWLLKEDVYKLAKHFNIPEIVITRAP 184

Query: 180 TAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVEN 233
           TA L+EGQTDE E+GI+YK LD+ L   + L  EK+KI  DL     K EH  N
Sbjct: 185 TASLFEGQTDETEMGITYKELDQYLKGDLILSSEKQKIVLDLK---AKAEHKHN 235


>gb|AAF22217.1|AF140575_2 (AF140575) NH(3)-dependent NAD+ synthetase [Mycoplasma
           hyopneumoniae]
           Length = 246
           
 Score =  164 bits (412), Expect = 7e-40
 Identities = 97/235 (41%), Positives = 138/235 (58%), Gaps = 18/235 (7%)

Query: 10  IERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYEN-QDVEDAK 68
           IE I   +++    GV+ G+SGGVDSA V  LA KA   +  LGLIMP  +   D+ D  
Sbjct: 15  IEWIRQEVKKAQKKGVIFGLSGGVDSALVAVLANKAFP-DSHLGLIMPIRDMITDMVDID 73

Query: 69  LVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANSMNRMVLG 128
           L+V+  GI+ + IN++P  + +     ++ +K +  N+  R RM  LYA A   + +VLG
Sbjct: 74  LLVKKFGIKNKEINLKPAFENLKKCFNLK-NKLANSNIQPRLRMTSLYAFAQEFDYLVLG 132

Query: 129 TSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQT 188
           T N SE   GYFTK+GDG  D  P++NL K +VW++A  IG+PE I+KKKP+A LWE QT
Sbjct: 133 TDNFSEMYLGYFTKYGDGGVDLLPIVNLTKVQVWKMAAEIGIPESIIKKKPSANLWENQT 192

Query: 189 DEDELGISYKLLDEILWRLVDLKMEKEK-IAEDLGIPIEKVEHVENLVKGSEHKR 242
           DE+E+G SY  L        DL ME    +++++   I K+  +      S HKR
Sbjct: 193 DENEMGFSYGDL--------DLFMENPNLVSKEIAAKITKLHEI------SSHKR 233


>pir||B81353 probable NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) Cj0810
           [similarity] - Campylobacter jejuni (strain NCTC 11168)
           >gi|6968256|emb|CAB73075.1| (AL139076) putative
           NH(3)-dependent NAD(+) synthetase [Campylobacter jejuni]
           Length = 246
           
 Score =  162 bits (405), Expect = 5e-39
 Identities = 95/248 (38%), Positives = 149/248 (59%), Gaps = 13/248 (5%)

Query: 4   LDYEKAIERIIDFIREKCSN----GVVVGISGGVDSATVTYLATKALGREKVLGLIMPYY 59
           +D++K  E++ DFI+EK  N    GVV+G+SGG+DSA V  L  +AL +E V  L+MP  
Sbjct: 1   MDWQKITEKMCDFIQEKVKNSQSQGVVLGLSGGIDSALVATLCKRAL-KENVFALLMPTQ 59

Query: 60  --ENQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYA 117
                ++EDA  +   L +EY++I I+ I+D  + +        SLGN  AR RM +LY 
Sbjct: 60  ISNKANLEDALRLCADLNLEYKIIEIQSILDAFIKQ-SENTTLVSLGNFAARIRMSLLYD 118

Query: 118 HANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKK 177
           ++   N +V+GTSN+SE L GY T +GD A  + P+ +LYK+E++ +AK + + E  +KK
Sbjct: 119 YSALKNSLVIGTSNKSELLLGYGTIYGDLACAFNPIGSLYKSEIYALAKYLNLHENFIKK 178

Query: 178 KPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKG 237
            P+A LWE Q+DE +LG SY  +DE    L  L+   EK+   L   +  +  ++N ++ 
Sbjct: 179 APSADLWENQSDEADLGFSYTKIDE---GLKALETNDEKLLRTLDPSL--IAMLKNRMQK 233

Query: 238 SEHKRRLP 245
           +  K ++P
Sbjct: 234 NAFKGKMP 241


>sp|P75216|NADE_MYCPN PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|2146516|pir||S73606
           probable NH(3)-dependent NAD(+) synthetase outB -
           Mycoplasma pneumoniae (strain ATCC 29342)
           >gi|1673951|gb|AAB95928.1| (AE000027) probable
           NH(3)-dependent NAD(+) synthetase [Mycoplasma
           pneumoniae]
           Length = 248
           
 Score =  156 bits (391), Expect = 2e-37
 Identities = 92/246 (37%), Positives = 143/246 (57%), Gaps = 18/246 (7%)

Query: 4   LDYEKAIER-IIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ 62
           L+Y   +E  + +++ E  + GVVVG+SGGVDSA V  + TK L  +  L L+M    ++
Sbjct: 6   LNYLNELEAWLTNYVNEAHAQGVVVGLSGGVDSAVVAAM-TKKLFPQNHLTLVMHINNSE 64

Query: 63  -DVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSL--GNVMARTRMMILYAHA 119
            D +    +VE L +  + +++ P    ++  + I+  K+ +  GN+ AR RM  LY HA
Sbjct: 65  LDHKATTALVEQLQLNNKQVDLEPPYRAMLQALTIDPQKELMVAGNLKARLRMACLYTHA 124

Query: 120 NSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKP 179
              N +VLGT N  E+  GYFTKWGDGA D APL  L K++V+ +++   VPE ++++ P
Sbjct: 125 QKHNYLVLGTGNFIEYSLGYFTKWGDGACDVAPLAFLLKSDVYALSQHFNVPELVIERAP 184

Query: 180 TAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSE 239
           TA L+ GQTDE E+G++YK LD+     + L   K+             + V++L + S+
Sbjct: 185 TASLFAGQTDEAEMGLTYKELDQYFQGHLQLSATKQ-------------QRVDHLRQSSQ 231

Query: 240 HKRRLP 245
           HKR LP
Sbjct: 232 HKRSLP 237


>sp|O67091|NADE_AQUAE PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(+) SYNTHASE
           [GLUTAMINE-HYDROLYSING]) >gi|7451134|pir||H70382
           NH(3)-dependent NAD+ synthetase - Aquifex aeolicus
           >gi|2983464|gb|AAC07044.1| (AE000715) NH(3)-dependent
           NAD+ synthetase [Aquifex aeolicus]
           Length = 567
           
 Score =  154 bits (386), Expect = 8e-37
 Identities = 94/246 (38%), Positives = 146/246 (59%), Gaps = 14/246 (5%)

Query: 15  DFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQDVEDAKLVVE 72
           D++R+     VV+G+SGG+DS+ V  LA  ALGRE V G+ MP  +   +  EDAK + +
Sbjct: 303 DYVRKNGFEKVVLGLSGGIDSSFVACLAVDALGRENVKGVYMPSQFSSKESYEDAKALAQ 362

Query: 73  SLGIEYRVINIRPIVDTIVSEIGIELDKKSLG----NVMARTRMMILYAHANSMNRMVLG 128
           +LGIE+ VI I+ I     +E   E+ + +      N+ AR R  IL+  +N    +VL 
Sbjct: 363 NLGIEFHVIPIKEIYRAYFNEFEKEICEITFDVADENIQARIRANILFYFSNKFRYLVLS 422

Query: 129 TSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK-----RIGVPERIVKKKPTAGL 183
           TSN+SE   GY T +GD A  +AP+ ++YKT V+++A+        +PER+ KK P+A L
Sbjct: 423 TSNKSETAVGYTTIYGDMAGGFAPIKDVYKTWVYKLARYRNSISPDIPERVFKKPPSAEL 482

Query: 184 WEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRR 243
              QTD+D L   Y++LD+IL   ++  +  E+I    G+P + V    N+++ +E+KR+
Sbjct: 483 RPNQTDQDVLP-PYEILDQILMLYIEENLSPEEIIRK-GLPRDAVYKTINMIRKNEYKRK 540

Query: 244 -LPIGP 248
             PIGP
Sbjct: 541 QAPIGP 546


>pir||S48586 hypothetical protein - Mycoplasma capricolum (SGC3) (fragment)
           >gi|7447178|pir||S77778 probable NH(3)-dependent NAD(+)
           synthetase outB - Mycoplasma capricolum (fragment)
           >gi|4545064|emb|CAA83707.1| (Z33030) ATP pyrophosphatase
           [Mycoplasma capricolum]
           Length = 253
           
 Score =  151 bits (377), Expect = 9e-36
 Identities = 98/250 (39%), Positives = 144/250 (57%), Gaps = 29/250 (11%)

Query: 10  IERIIDFIRE-----KCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ-D 63
           ++ +++FI++     KC +GVVVGISGG+DSA V  LA +A   +  L + MP Y +Q D
Sbjct: 17  LDYLVEFIQQTVKKAKC-DGVVVGISGGIDSAVVANLAKRAFP-DNYLTVWMPIYSSQLD 74

Query: 64  VEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKK----SLGNVMARTRMMILYAHA 119
            +    ++++  ++   +N+    D   +     LD+K    ++ N  AR RM  LY  A
Sbjct: 75  YDCVNELIKTNQLKNIEVNLEASFDAFKNSFS-NLDEKPNLLAISNSKARLRMTTLYTIA 133

Query: 120 NSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKP 179
            +   +VLGT N  E+  GYFTK+GDG  D  P+I+L K+EV + AK + VPE I+ +KP
Sbjct: 134 QTKKYLVLGTDNLDEWHIGYFTKYGDGGVDVVPIIHLLKSEVKKAAKILNVPELIINRKP 193

Query: 180 TAGLWEGQTDEDELGISYKLLDE-ILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGS 238
           TAGLWEGQTDE E+G SY L+D  +L +  D K++K                ++ L K S
Sbjct: 194 TAGLWEGQTDEGEIGFSYDLIDSYLLKQNNDPKLKK---------------RIDYLHKIS 238

Query: 239 EHKRRLPIGP 248
           +HKR L I P
Sbjct: 239 KHKRMLAIKP 248


>sp|Q9WZB3|NAE1_THEMA NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|7448977|pir||D72351
           hypothetical protein TM0645 - Thermotoga maritima
           (strain MSB8) >gi|4981167|gb|AAD35729.1|AE001738_9
           (AE001738) NH(3)-dependent NAD(+) synthetase, putative
           [Thermotoga maritima]
           Length = 281
           
 Score =  150 bits (374), Expect = 2e-35
 Identities = 103/281 (36%), Positives = 151/281 (53%), Gaps = 52/281 (18%)

Query: 11  ERIIDFIREKCSN----GVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ--DV 64
           E +I FI+EK       G VVG+SGGVDSA V  L  +ALG+++V  LI+P  ++    +
Sbjct: 3   ESLISFIKEKIEEYNYRGAVVGVSGGVDSAVVLSLCVQALGKDRVFALILPERDSSKDSL 62

Query: 65  EDAKLVVESLGIEYRVINIRPIV----------------DTIVSEIGIEL---------- 98
           +DA    E LG+EYR  +I PI+                D+IV    +            
Sbjct: 63  KDAVDFCERLGVEYRKRSITPILRKIGVYRLFPPRLFLPDSIVKRYVLNRWNTLSKDPFL 122

Query: 99  -DKKSLG-----------NVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDG 146
            D ++ G            +  R RM +LY  A      V+GT+NR+E+LTG + KWGD 
Sbjct: 123 DDLRNTGPEEFLKGLAYYRIKHRIRMCLLYFEAEKRGYAVVGTTNRTEYLTGLYVKWGDE 182

Query: 147 ASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQTDEDELGISYKLLDEILWR 206
           A D  P+++LYKT+V+E+AK + VPE+I+KK P+  L  G TDE    +SY  LD IL  
Sbjct: 183 AVDIEPIMHLYKTQVFELAKEMNVPEKILKKPPSPDLIPGITDEMAFNMSYLELDRIL-- 240

Query: 207 LVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSE-HKRRLPI 246
              +K+EK +   D     +KVE V+ +++ SE ++R +PI
Sbjct: 241 ---MKLEKNEDLSD--EDPKKVERVKKILELSEKYRRDIPI 276


>sp|Q9X0Y0|NAE2_THEMA PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(+) SYNTHASE
           [GLUTAMINE-HYDROLYSING]) >gi|7451135|pir||G72277
           NH(3)-dependent NAD(+) synthetase - Thermotoga maritima 
           (strain MSB8) >gi|4981808|gb|AAD36328.1|AE001780_12
           (AE001780) NH(3)-dependent NAD(+) synthetase [Thermotoga
           maritima]
           Length = 576
           
 Score =  137 bits (342), Expect = 1e-31
 Identities = 89/252 (35%), Positives = 144/252 (56%), Gaps = 17/252 (6%)

Query: 6   YEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQD 63
           +   I  + D++R+     VV+G+SGG+DS+ V  +AT+ALG+E V G++MP  Y   + 
Sbjct: 299 FRALITGLRDYVRKNGFEKVVIGLSGGMDSSLVAVIATEALGKENVKGVLMPSMYTSKES 358

Query: 64  VEDAKLVVESLGIEYRVINIRPIVDTIVSEI-----GIELDKKSLGNVMARTRMMILYAH 118
           +EDA+ + ++LGIE  +I I  +  + +  +     G E D     N+ AR R   L A 
Sbjct: 359 IEDAQTLAKNLGIETFIIPITDVFHSYLETLKGVFAGREPDITE-ENLQARIRGNYLMAL 417

Query: 119 ANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIG-------VP 171
           +N    +VL T N+SE  TGY T +GD A  +A + ++YKT+V+ I +          +P
Sbjct: 418 SNKFGWLVLTTGNKSEMATGYATLYGDMAGGFAVIKDVYKTDVYRIGRWYNSWRGKEIIP 477

Query: 172 ERIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHV 231
           E I  K PTA L  GQTD+++L   Y++LDEIL   ++  ++ E+IA   G   + V  V
Sbjct: 478 ENIFVKPPTAELRPGQTDQEKLP-PYEVLDEILRLYIEEGLDPEEIASK-GFDRKTVLDV 535

Query: 232 ENLVKGSEHKRR 243
             +++ +E+KR+
Sbjct: 536 TEMIRKNEYKRK 547


>sp|O25096|NADE_HELPY NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|7448974|pir||A64561
           NH(3)-dependent NAD+ synthetase - Helicobacter pylori
           (strain 26695) >gi|2313427|gb|AAD07396.1| (AE000550)
           NH(3)-dependent NAD+ synthetase (nadE) [Helicobacter
           pylori 26695]
           Length = 260
           
 Score =  128 bits (318), Expect = 8e-29
 Identities = 86/251 (34%), Positives = 138/251 (54%), Gaps = 13/251 (5%)

Query: 5   DYEKAIERIIDFIREKCSNG----VVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
           DY+K I  + DF+ ++        VV G+SGG+DSA V  L  K   +E    L+MP   
Sbjct: 4   DYQKLIVYLCDFLEKEVQKRGFKKVVYGLSGGLDSAVVGVLCQKVF-KENAHALLMPSSV 62

Query: 61  N--QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAH 118
           +  ++  DA  + E   I Y   +I P  D I S    +      GN  AR RM  LY +
Sbjct: 63  SMPENKTDALNLCEKFSIPYTEYSIAPY-DAIFSSHFKDASLTRKGNFCARLRMAFLYDY 121

Query: 119 ANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKK 178
           +   + +V+GTSN+SE + GY T +GD A    P+  L+KTEV+E+A+R+ +P++I+ K 
Sbjct: 122 SLKSDSLVIGTSNKSERMLGYGTLFGDLACAINPIGELFKTEVYELARRLNIPKKILNKP 181

Query: 179 PTAGLWEGQTDEDELGISYKLLDEILWRLVDL----KMEKEKIAEDLGIPIEKVEHVENL 234
           P+A L+ GQ+DE +LG  Y ++D +L  +  L     ++ E +A+ LG     V+++ + 
Sbjct: 182 PSADLFVGQSDEKDLGYPYSVIDPLLKDIEALFQTKPIDTETLAQ-LGYDEILVKNITSR 240

Query: 235 VKGSEHKRRLP 245
           ++ +  K  LP
Sbjct: 241 IQKNAFKLELP 251


>sp|P08164|NADE_BACSU NH(3)-DEPENDENT NAD(+) SYNTHETASE (SPORE OUTGROWTH FACTOR B)
           (SPORULATION PROTEIN OUTB) (GENERAL STRESS PROTEIN 38)
           (GSP38) >gi|80412|pir||A26936 NAD+ synthase (EC 6.3.1.5)
           [validated] - Bacillus subtilis
           >gi|143279|gb|AAA22635.1| (M15811) outB [Bacillus
           subtilis] >gi|1805385|dbj|BAA08947.1| (D50453) spore
           outgrowth factor B [Bacillus subtilis]
           >gi|2632599|emb|CAB12107.1| (Z99105) NH3-dependent NAD+
           synthetase [Bacillus subtilis]
           Length = 272
           
 Score =  122 bits (303), Expect = 4e-27
 Identities = 91/269 (33%), Positives = 141/269 (51%), Gaps = 34/269 (12%)

Query: 3   TLDYEKAIERIIDFIREKC----SNGVVVGISGGVDSATVTYLATKAL-------GREKV 51
           ++D ++ IE  ++F+++      + G V+GISGG DS     LA  A+       G  + 
Sbjct: 16  SIDPKQEIEDRVNFLKQYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVESIREEGGDAQF 75

Query: 52  LGLIMPYYENQDVEDAKLVVESLGIEYR-VINIRPIV----DTIVSEIGIELDKKSLGNV 106
           + + +P+   QD +DA+L ++ +  +     +I+  V    D    E G +L   + GNV
Sbjct: 76  IAVRLPHGTQQDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNV 135

Query: 107 MARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK 166
            ARTRM+  YA       +VLGT + +E +TG+FTK+GDG +D  PL  L K +   + K
Sbjct: 136 KARTRMIAQYAIGGQEGLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLK 195

Query: 167 RIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGI 223
            +G PER+  K+PTA L +    Q+DE ELGISY  +D+ L    + K    K++E L  
Sbjct: 196 ELGAPERLYLKEPTADLLDEKPQQSDETELGISYDEIDDYL----EGKEVSAKVSEAL-- 249

Query: 224 PIEKVEHVENLVKGSEHKRRLPIGPSFDD 252
                   E     +EHKR++P    FDD
Sbjct: 250 --------EKRYSMTEHKRQVP-ASMFDD 269


>pdb|2NSY|A Chain A, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis In Complex With Nad-Adenylate
           >gi|4389118|pdb|2NSY|B Chain B, Crystal Structure Of
           Nh3-Dependent Nad+ Synthetase From Bacillus Subtilis In
           Complex With Nad-Adenylate >gi|2392477|pdb|1NSY|A Chain
           A, Crystal Structure Of Nh3-Dependent Nad+ Synthetase
           From Bacillus Subtilis >gi|2392478|pdb|1NSY|B Chain B,
           Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
           Bacillus Subtilis
           Length = 271
           
 Score =  122 bits (303), Expect = 4e-27
 Identities = 91/269 (33%), Positives = 141/269 (51%), Gaps = 34/269 (12%)

Query: 3   TLDYEKAIERIIDFIREKC----SNGVVVGISGGVDSATVTYLATKAL-------GREKV 51
           ++D ++ IE  ++F+++      + G V+GISGG DS     LA  A+       G  + 
Sbjct: 15  SIDPKQEIEDRVNFLKQYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVESIREEGGDAQF 74

Query: 52  LGLIMPYYENQDVEDAKLVVESLGIEYR-VINIRPIV----DTIVSEIGIELDKKSLGNV 106
           + + +P+   QD +DA+L ++ +  +     +I+  V    D    E G +L   + GNV
Sbjct: 75  IAVRLPHGTQQDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNV 134

Query: 107 MARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK 166
            ARTRM+  YA       +VLGT + +E +TG+FTK+GDG +D  PL  L K +   + K
Sbjct: 135 KARTRMIAQYAIGGQEGLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLK 194

Query: 167 RIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGI 223
            +G PER+  K+PTA L +    Q+DE ELGISY  +D+ L    + K    K++E L  
Sbjct: 195 ELGAPERLYLKEPTADLLDEKPQQSDETELGISYDEIDDYL----EGKEVSAKVSEAL-- 248

Query: 224 PIEKVEHVENLVKGSEHKRRLPIGPSFDD 252
                   E     +EHKR++P    FDD
Sbjct: 249 --------EKRYSMTEHKRQVP-ASMFDD 268


>sp|P74292|NADE_SYNY3 PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(+) SYNTHASE
           [GLUTAMINE-HYDROLYSING]) >gi|7451133|pir||S75927
           hypothetical protein - Synechocystis sp. (strain PCC
           6803) >gi|1653472|dbj|BAA18386.1| (D90913)
           NH(3)-dependent NAD(+) synthetase [Synechocystis sp.]
           Length = 558
           
 Score =  122 bits (302), Expect = 6e-27
 Identities = 80/240 (33%), Positives = 130/240 (53%), Gaps = 13/240 (5%)

Query: 15  DFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQDVEDAKLVVE 72
           D++ +   +  ++G+SGG+DS+ V  +A +ALG+E VLG++MP  Y  +  ++DA  + +
Sbjct: 291 DYLHKCGFSKAILGLSGGIDSSLVAAIAVEALGKENVLGILMPSPYSSDHSIQDALALAK 350

Query: 73  SLGIEYRVINIRPIVDTIVSEIGIELDKKSLG----NVMARTRMMILYAHANSMNRMVLG 128
           +LG+  + I I PI+ T    +         G    N+ +R R  +L A AN    ++L 
Sbjct: 351 NLGMNTQTIPIGPIMATYDQALVPLFQDAPFGLAEENLQSRIRGNLLMAIANKFGHLLLS 410

Query: 129 TSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIG-----VPERIVKKKPTAGL 183
           T N+SE   GY T +GD     A + ++ KT+V+E+ + +      +P  ++ K P+A L
Sbjct: 411 TGNKSELAVGYCTLYGDMNGGLAAIADVPKTQVFELCRWLNREQTIIPPSVLTKPPSAEL 470

Query: 184 WEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRR 243
             GQ D D L   Y +LD IL RLV+     ++I  + G   E V  +  LV+ SE KRR
Sbjct: 471 KPGQVDTDSLP-PYDVLDGILGRLVEKHQSPQEII-NAGFEREVVLKICQLVQKSEFKRR 528


>emb|CAB58266.1| (AL121849) NH(3)-dependent NAD(+)synthetase (EC 6.3.5.1)
           [Streptomyces coelicolor A3(2)]
           Length = 276
           
 Score =  121 bits (300), Expect = 1e-26
 Identities = 85/270 (31%), Positives = 138/270 (50%), Gaps = 39/270 (14%)

Query: 3   TLDYEKAIERIIDFIREKCSN----GVVVGISGGVDSATVTYLATKALGREKVLG----- 53
           T D E+ IER + F+ E+ ++     +V+GISGGVDS T   L   A+ R +  G     
Sbjct: 22  TFDAEREIERRVAFLAERLTSTGLRALVLGISGGVDSTTAGRLCQLAVERARAAGHEALF 81

Query: 54  --LIMPYYENQDVEDAKLVVESLGIE-YRVINIRPIVDTIVSEIGIELDKKSL------- 103
             + +P+    D +DA+  +  +  +    ++I+P  D  +  + +  D           
Sbjct: 82  YAMRLPHGVQADEDDAQQALSFIRPDRVLTVDIKPASDAALDAL-LAADVAFRDPHHQDF 140

Query: 104 --GNVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEV 161
             GN+ AR RM+  YA A +   +V+GT + +E ++G+FTK+GDGA+D  PL  L K  V
Sbjct: 141 VHGNIKARQRMIAQYAVAGAHGGLVVGTDHAAEAVSGFFTKFGDGAADVVPLTGLTKRRV 200

Query: 162 WEIAKRIGVPERIVKKKPTAGLW---EGQTDEDELGISYKLLDEILWRLVDLKMEKEKIA 218
             +   +G P  +V+K PTA L     G+ DED LG++Y+ +D+ L        E + + 
Sbjct: 201 RAVGDALGAPAALVRKVPTADLETLDPGKADEDALGVTYEEIDDFL--------EGKPVT 252

Query: 219 EDLGIPIEKVEHVENLVKGSEHKRRLPIGP 248
           E +       E +    + ++HKRRLPI P
Sbjct: 253 EQV------FETIVTRYRQTDHKRRLPIAP 276


>sp|Q9ZMB0|NADE_HELPJ NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|7448973|pir||A71949
           nh(3)-dependent nad+ synthetase - Helicobacter pylori
           (strain J99) >gi|4154831|gb|AAD05885.1| (AE001467)
           NH(3)-DEPENDENT NAD+ SYNTHETASE [Helicobacter pylori
           J99]
           Length = 260
           
 Score =  120 bits (298), Expect = 2e-26
 Identities = 85/250 (34%), Positives = 131/250 (52%), Gaps = 11/250 (4%)

Query: 5   DYEKAIERIIDF----IREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
           DY+K I  + DF    +++K    VV G+SGG+DSA V  L  K   +E    L+MP   
Sbjct: 4   DYQKLIVYLCDFLEKEVQKKGFKKVVYGLSGGLDSAVVGVLCQKVF-KENAHALLMPSSV 62

Query: 61  N--QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAH 118
           +  +   DA  + E   I Y   +I P  D I      +      GN  AR RM  LY +
Sbjct: 63  SMPESRTDALNLCEKFSIPYTEYSIAPY-DKIFGSHFKDASLTRKGNFCARLRMAFLYDY 121

Query: 119 ANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKK 178
           +   + +V+GTSN+SE + GY T +GD A    P+  L+KTEV+E+A  + +P++I+ K 
Sbjct: 122 SLKSDSLVIGTSNKSERMLGYGTLFGDLACAINPIGELFKTEVYELAYYLNIPKKILNKP 181

Query: 179 PTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAE---DLGIPIEKVEHVENLV 235
           P+A L+ GQ+DE +LG  Y ++D +L  +  L   K    E    LG     V+++ + +
Sbjct: 182 PSADLFVGQSDEKDLGYPYSVIDPLLKDIEALFQTKPIHLETLIQLGYAEILVKNIISRI 241

Query: 236 KGSEHKRRLP 245
           + +  K  LP
Sbjct: 242 QKNAFKLELP 251


>pir||T51756 NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) [imported] -
           Streptomyces coelicolor (fragment)
           >gi|4469317|emb|CAB38325.1| (Y17736) NAD(+) synthase
           (glutamine-hydrolysing) [Streptomyces coelicolor A3(2)]
           Length = 538
           
 Score =  120 bits (297), Expect = 2e-26
 Identities = 80/251 (31%), Positives = 121/251 (47%), Gaps = 17/251 (6%)

Query: 6   YEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQD 63
           Y   +  +  ++ +     V++G+SGG+DSA V  +A  ALG E V G+ MP  Y     
Sbjct: 263 YSALVTGLRAYVAKNGFRSVLIGLSGGIDSALVASIACDALGAEHVYGVSMPSKYSSGHS 322

Query: 64  VEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANSMN 123
            +DA  +    G+ YR ++I P+ D       +EL   +  N+ +R R  +L A +N   
Sbjct: 323 RDDAAELARRTGLHYRTVSIEPMFDAYTG--ALELTGLAEENLQSRLRGTLLMALSNQEG 380

Query: 124 RMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIA-----------KRIGVPE 172
            +VL   N+SE   GY T +GD    Y P+ ++YKT V+ +A           +   VPE
Sbjct: 381 HLVLAPGNKSELAVGYSTLYGDSVGGYGPIKDVYKTSVFRLAEWRNRAARDRGRTPPVPE 440

Query: 173 RIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVE 232
             + K P+A L  GQ D D L   Y +LD +L   VD     ++I    G   E V    
Sbjct: 441 NSITKPPSAELRPGQVDTDSLP-DYPVLDAVLALYVDRDRGADEIVA-AGYDRELVTRTL 498

Query: 233 NLVKGSEHKRR 243
            +V  +E+KRR
Sbjct: 499 RMVDTAEYKRR 509


>pir||C82617 NH3-dependent NAD synthetase XF1961 [imported] - Xylella fastidiosa
           (strain 9a5c) >gi|9107063|gb|AAF84763.1|AE004015_6
           (AE004015) NH3-dependent NAD synthetase [Xylella
           fastidiosa]
           Length = 545
           
 Score =  120 bits (297), Expect = 2e-26
 Identities = 81/255 (31%), Positives = 137/255 (52%), Gaps = 15/255 (5%)

Query: 1   MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
           +  L +   +  + D+ R+   + V VG+SGG+DSA V  +A  ALG ++V  + +P   
Sbjct: 265 INALVWRAVVRGVQDYCRKNGFSKVWVGLSGGIDSALVLAIAVDALGADQVTAVRLPSRY 324

Query: 61  NQDVED--AKLVVESLGIEYRVINIRPIVDTIVSEIGIEL----DKKSLGNVMARTRMMI 114
             ++ +  A     SLG+    + I P+ + +++ +G          +  N+ +R+R +I
Sbjct: 325 TAELSNDLAAEQCHSLGVRLETVAIEPVFEGLLAALGPLFAGMAPDATEENLQSRSRGVI 384

Query: 115 LYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK-------R 167
           L A AN    ++L T N+SE+  GY T +GD    YAPL ++YK++V+E+A+        
Sbjct: 385 LMALANKFGGLLLTTGNKSEYAVGYATIYGDMCGGYAPLKDIYKSQVFELAQWRNTVSDV 444

Query: 168 IGVPERIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEK 227
           + +P  ++ + P+A L   QTD+D L   Y++LD IL   VD +  +E I    G     
Sbjct: 445 LAIPPGVIHRPPSAELRAQQTDQDSLP-PYEVLDGILSLYVDQEQSREDIIA-AGYAAGV 502

Query: 228 VEHVENLVKGSEHKR 242
           V++V NLVK +E KR
Sbjct: 503 VDYVLNLVKINEWKR 517


>pir||H82488 NH(3)-dependent NAD(+) synthetase VCA0207 [imported] - Vibrio
           cholerae (group O1 strain N16961)
           >gi|9657599|gb|AAF96119.1| (AE004360) NH(3)-dependent
           NAD(+) synthetase [Vibrio cholerae]
           Length = 276
           
 Score =  119 bits (296), Expect = 3e-26
 Identities = 89/278 (32%), Positives = 144/278 (51%), Gaps = 44/278 (15%)

Query: 3   TLDYEKAIERIIDFIREKCSNG----VVVGISGGVDSAT---VTYLATKALGRE------ 49
           ++D +  IER + FI+ K +      +V+GISGGVDS T   +  LA + L ++      
Sbjct: 14  SIDPQFEIERRVAFIKRKLTEARCKSLVLGISGGVDSTTCGRLAQLAVEELNQQHNTTEY 73

Query: 50  KVLGLIMPYYENQDVEDAKLVVESLGIEYRV-INIRPIVDTIVSEIGIELDKKSL----- 103
           + + + +PY E +D ++A+L +  +   + V +NI+  VD + +     L    L     
Sbjct: 74  QFIAVRLPYGEQKDEDEAQLALSFIRPTHSVSVNIKAGVDGLHAASHHALANTGLIPSDP 133

Query: 104 -------GNVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINL 156
                  GNV AR RM+  Y  A  +  +VLGT + +E +TG++TK+GDGA D APL  L
Sbjct: 134 AKVDFIKGNVKARARMVAQYEIAGYVGGLVLGTDHSAENITGFYTKFGDGACDLAPLFGL 193

Query: 157 YKTEVWEIAKRIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKME 213
            K +V  +AK +G PE++V K PTA L E    + DE  L ++Y+ +D+ L         
Sbjct: 194 NKRQVRLLAKTLGAPEQLVYKTPTADLEELAPQKADEAALNLTYEQIDDFL--------- 244

Query: 214 KEKIAEDLGIPIEKVEHVENLVKGSEHKRRLPIGPSFD 251
                E   +P E  + +  +   ++HKR+ PI   +D
Sbjct: 245 -----EGKAVPAEVSQRLVAIYHATQHKRQ-PIPTIYD 276


>pir||F83030 NH3-dependent NAD synthetase PA4920 [imported] - Pseudomonas
           aeruginosa (strain PAO1)
           >gi|9951198|gb|AAG08305.1|AE004905_3 (AE004905)
           NH3-dependent NAD synthetase [Pseudomonas aeruginosa]
           Length = 275
           
 Score =  119 bits (295), Expect = 4e-26
 Identities = 87/267 (32%), Positives = 136/267 (50%), Gaps = 37/267 (13%)

Query: 5   DYEKAIERIIDFIREKCSNG----VVVGISGGVDSATVTYLATKALGR---------EKV 51
           D +  I R I FI++   +     +V+GISGGVDS T   LA +A+ +          + 
Sbjct: 23  DVQAQIARRIAFIQQCLKDSGLKTLVLGISGGVDSLTAGLLAQRAVEQLREQTGDQAYRF 82

Query: 52  LGLIMPYYENQDVEDAKLVVESLGI-EYRVINIRPIVDTIVSEI----GIELDKKS--LG 104
           + + +PY   QD  DA+  + ++   E + +NI P V  +  ++    G+E  K    +G
Sbjct: 83  IAVRLPYQVQQDEADAQASLATIRADEEQTVNIGPSVKALAEQLEALEGLEPAKSDFVIG 142

Query: 105 NVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEI 164
           N+ AR RM+  YA A +   +V+GT + +E + G+FTK+GDGA D APL  L K +V  +
Sbjct: 143 NIKARIRMVAQYAIAGARGGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLAKHQVRAL 202

Query: 165 AKRIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDL 221
           A+ +G PE +V+K PTA L +   G  DE   G++Y  +D  L          + + E+ 
Sbjct: 203 ARALGAPENLVEKIPTADLEDLRPGHPDEASHGVTYAEIDAFL--------HGQPLREEA 254

Query: 222 GIPIEKVEHVENLVKGSEHKRRLPIGP 248
              I    H       ++HKR LP  P
Sbjct: 255 ARVIVDTYH------KTQHKRELPKAP 275


>pir||A75617 NH3-dependent NAD+ synthase - Deinococcus radiodurans  (strain R1)
           >gi|6460473|gb|AAF12179.1|AE001862_5 (AE001862)
           NH3-dependent NAD+ synthase [Deinococcus radiodurans]
           Length = 287
           
 Score =  117 bits (290), Expect = 1e-25
 Identities = 82/259 (31%), Positives = 125/259 (47%), Gaps = 29/259 (11%)

Query: 5   DYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREK-------VLGLIMP 57
           + E+ +  + D+++   + G V+GISGG DS     L   A+ R +        L + +P
Sbjct: 30  EVERRVAFLCDYLQSTPTKGFVLGISGGQDSTLAGRLCQLAVERRRSQGHGATFLAVRLP 89

Query: 58  YYENQDVEDAKLVVESLGIEYRV-INIRPIVDTIVSE----IGIELDKKSLGNVMARTRM 112
           Y    D  DA+  ++ +  +  V +NI+   D  V+     +G E+     GNV AR RM
Sbjct: 90  YGVQADEADAQQALDFIQADREVTVNIKEAADASVAAAQAALGSEVRDFVRGNVKARERM 149

Query: 113 MILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPE 172
           +  YA A   N +V+GT + +E LTG++TK+GDG  D  PL  L K +  ++   +G PE
Sbjct: 150 VAQYALAGQENLLVVGTDHAAEALTGFYTKYGDGGVDLTPLSGLTKRQGAQLLAHLGAPE 209

Query: 173 RIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVE 229
              +K PTA L +   G  DE  LG++Y  +D  L              E   +  E   
Sbjct: 210 GTWRKVPTADLEDDRPGLPDEVALGVTYAQIDAYL--------------EGREVSDEAAA 255

Query: 230 HVENLVKGSEHKRRLPIGP 248
            +E L   S HKR LP+ P
Sbjct: 256 RLERLFLNSRHKRALPVTP 274


>pir||C81850 NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) NMA1576
           [similarity] - Neisseria meningitidis (group A strain
           Z2491) >gi|7380217|emb|CAB84803.1| (AL162756)
           NH(3)-dependent NAD synthetase [Neisseria meningitidis
           Z2491]
           Length = 273
           
 Score =  117 bits (290), Expect = 1e-25
 Identities = 86/255 (33%), Positives = 136/255 (52%), Gaps = 24/255 (9%)

Query: 4   LDYEKAIERIIDFIREKCSN----GVVVGISGGVDSATVTYLATKALGREKVLGLIMPYY 59
           +D +  I  I+ ++ E  +     G VVG+SGG+DSA V+ LA +  GR  +L L MP  
Sbjct: 11  MDTQAVITHIVRWLDEYAARANAKGFVVGVSGGIDSAVVSALAART-GRTTLL-LDMPIR 68

Query: 60  ENQD-VEDAKLVVESLGIEYRVINIRPI--VDTI--------VSEIGIELDKKSLGNVMA 108
           ++ D +E A+L + +L  +Y  ++ + +   DT         V +   +    SL N  +
Sbjct: 69  QHPDQLERARLHIRNLQRQYANVSAQTVDLTDTFQTFEQTVGVHQTAFDNQPLSLANARS 128

Query: 109 RTRMMILYAHANSMNRMVLGTSNRSE-FLTGYFTKWGDGASDYAPLINLYKTEVWEIAKR 167
           R RM+ LY +      +V GT N+ E F  G+FTK+GDG  D +P+ +L KT+V+ +A+ 
Sbjct: 129 RLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGFFTKYGDGGVDISPIADLTKTQVYRLAEA 188

Query: 168 IGVPERIVKKKPTAGLWEGQ-TDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIE 226
           +GV E I K  PT GLW+ + TDE+++G SY    E+ W +      K +  E  G   E
Sbjct: 189 LGVDEAIQKAPPTDGLWDTERTDEEQMGASY---PELEWAMGVYGTRKPEDFE--GRQRE 243

Query: 227 KVEHVENLVKGSEHK 241
            +E    L +  +HK
Sbjct: 244 VLEIYTRLHRAMQHK 258


>sp|Q03638|NADE_RHOCA GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(+) SYNTHASE
           [GLUTAMINE-HYDROLYSING]) >gi|97438|pir||S15555 NAD+
           synthase (EC 6.3.1.5) adgA [validated] - Rhodobacter
           capsulatus >gi|45986|emb|CAA42042.1| (X59399) NAD
           synthetase [Rhodobacter capsulatus]
           Length = 552
           
 Score =  114 bits (282), Expect = 1e-24
 Identities = 87/259 (33%), Positives = 131/259 (49%), Gaps = 24/259 (9%)

Query: 5   DYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQ 62
           DY   +  + D++R+   + VV+G+SGG+DSA V  +A  ALG   V  +++P  Y    
Sbjct: 267 DYRAMVLGLQDYLRKSGFSRVVLGLSGGIDSALVAVIAADALGAGNVHCVMLPSRYTSQG 326

Query: 63  DVEDAKLVVESLGIEYRVINI---RPIVDTIVSEI--GIELDKKSLGNVMARTRMMILYA 117
            ++DA  +   LG     + I   R  V+  ++ +  G   D     N+ +R R +IL A
Sbjct: 327 SLDDAADLARRLGARLDTVEIEGPRAAVEGALAHVLAGTAPDVTE-ENIQSRLRGVILMA 385

Query: 118 HANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIG-------- 169
            +N    M+L T N+SE   GY T +GD A  Y PL +LYKT V+E  +           
Sbjct: 386 ISNKFGAMLLTTGNKSEVAVGYCTIYGDMAGGYNPLKDLYKTRVFETCRWRNATHRPWMQ 445

Query: 170 ------VPERIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGI 223
                 +P  I+ K P+A L E QTD+D L   Y++LD IL RLV+     ++I    G 
Sbjct: 446 APAGEIIPVAIIDKPPSAELRENQTDQDSLP-PYEVLDAILERLVEGDQSVDQIVA-AGF 503

Query: 224 PIEKVEHVENLVKGSEHKR 242
               V+ +E+L+  SE KR
Sbjct: 504 DRATVKRIEHLLYISEWKR 522


>pir||A81090 NH(3)-dependent NAD+ synthetase NadE, probable NMB1364 [imported] -
           Neisseria meningitidis (group B strain MD58)
           >gi|7226610|gb|AAF41738.1| (AE002485) NH(3)-dependent
           NAD+ synthetase NadE, putative [Neisseria meningitidis
           MC58]
           Length = 273
           
 Score =  112 bits (278), Expect = 4e-24
 Identities = 80/233 (34%), Positives = 125/233 (53%), Gaps = 20/233 (8%)

Query: 22  SNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ-DVEDAKLVVESLGIEY-- 78
           + G VVG+SGG+DSA V+ LA +  GR  +L L MP  ++   +E A+L + +L  +Y  
Sbjct: 33  AKGFVVGVSGGIDSAVVSALAART-GRPTLL-LDMPIRQHPGQLERARLHIRNLQRQYAN 90

Query: 79  ---RVINIRPIVDTIVSEIG-----IELDKKSLGNVMARTRMMILYAHANSMNRMVLGTS 130
              + +++     T    +G      +    SL N  +R RM+ LY +      +V GT 
Sbjct: 91  VSAQTVDLTDTFQTFEQTVGAHQTAFDSQPLSLANARSRLRMLTLYYYGQIHGLLVTGTG 150

Query: 131 NRSE-FLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQ-T 188
           N+ E F  G+FTK+GDG  D +P+ +L KT+V+ +A+ +GV E I K  PT GLW+ + T
Sbjct: 151 NKIEDFGVGFFTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERT 210

Query: 189 DEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHK 241
           DE+++G SY    E+ W +      K +  E  G   E +E    L +  +HK
Sbjct: 211 DEEQMGASY---PELEWAMGVYGTRKPEDFE--GRQREVLEIYTRLHRAMQHK 258


>sp|P18843|NADE_ECOLI NH(3)-DEPENDENT NAD(+) SYNTHETASE (NITROGEN-REGULATORY PROTEIN)
           >gi|7443246|pir||D64933 NAD+ synthase (EC 6.3.1.5) nadE
           [validated] - Escherichia coli (strain K-12)
           >gi|1742846|dbj|BAA15529.1| (D90817) NH(3)-dependent
           NAD(+) synthetase (EC 6.3.5.1) (Nitrogen-regulatory
           protein). [Escherichia coli] >gi|1742853|dbj|BAA15535.1|
           (D90818) NH(3)-dependent NAD(+) synthetase (EC 6.3.5.1)
           (Nitrogen-regulatory protein). [Escherichia coli]
           >gi|1788036|gb|AAC74810.1| (AE000269) NAD synthetase,
           prefers NH3 over glutamine [Escherichia coli K12]
           Length = 275
           
 Score =  109 bits (270), Expect = 3e-23
 Identities = 85/272 (31%), Positives = 132/272 (48%), Gaps = 40/272 (14%)

Query: 4   LDYEKAIERIIDFIREKCSN-----GVVVGISGGVDSATVTYLATKAL-------GREKV 51
           ++ E+ I R +DF++           +V+GISGG DS     L   A+       G E +
Sbjct: 17  INAEEEIRRSVDFLKSYLQTYPFIKSLVLGISGGQDSTLAGKLCQMAINELRLETGNESL 76

Query: 52  --LGLIMPY---YENQDVEDAKLVVESLGIEYRVINIRPIV---DTIVSEIGIELDKKSL 103
             + + +PY    + QD +DA   ++   +    +NI+  V   +  + E GIEL     
Sbjct: 77  QFIAVRLPYGVQADEQDCQDAIAFIQPDRV--LTVNIKGAVLASEQALREAGIELSDFVR 134

Query: 104 GNVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWE 163
           GN  AR RM   Y+ A   + +V+GT + +E +TG+FTK+GDG +D  PL  L K +  +
Sbjct: 135 GNEKARERMKAQYSIAGMTSGVVVGTDHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQ 194

Query: 164 IAKRIGVPERIVKKKPTAGLWEGQ---TDEDELGISYKLLDEILWRLVDLKMEKEKIAED 220
           +   +  PE + KK PTA L + +    DE  LG++Y  +D+ L              E 
Sbjct: 195 LLAALACPEHLYKKAPTADLEDDRPSLPDEVALGVTYDNIDDYL--------------EG 240

Query: 221 LGIPIEKVEHVENLVKGSEHKRRLPIGPSFDD 252
             +P +    +EN    +EHKRR PI   FDD
Sbjct: 241 KNVPQQVARTIENWYLKTEHKRRPPI-TVFDD 271


>pir||A26928 nitrogen-regulatory protein - Escherichia coli
           >gi|146974|gb|AAA79852.1| (M15328) NH3-dependent NAD
           synthetase [Escherichia coli]
           Length = 274
           
 Score =  104 bits (257), Expect = 1e-21
 Identities = 80/246 (32%), Positives = 121/246 (48%), Gaps = 35/246 (14%)

Query: 25  VVVGISGGVDSATVTYLATKAL-------GREKV--LGLIMPY---YENQDVEDAKLVVE 72
           +V+GISGG DS     L   A+       G E +  + + +PY    + QD +DA   ++
Sbjct: 42  LVLGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ 101

Query: 73  SLGIEYRVINIRPIV---DTIVSEIGIELDKKSLGNVMARTRMMILYAHANSMNRMVLGT 129
              +    +NI+  V   +  + E GIEL     GN  AR RM   Y+ A   + +V+GT
Sbjct: 102 PDRV--LTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT 159

Query: 130 SNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQ-- 187
            + +E +TG+FTK+GDG +D  PL  L K +  ++   +  PE + KK PTA L + +  
Sbjct: 160 DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDRPS 219

Query: 188 -TDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRRLPI 246
             DE  LG++Y  +D+ L              E   +P +    +EN    +EHKRR PI
Sbjct: 220 LPDEVALGVTYDNIDDYL--------------EGKNVPQQVARTIENWYLKTEHKRRPPI 265

Query: 247 GPSFDD 252
              FDD
Sbjct: 266 -TVFDD 270


>dbj|BAB06004.1| (AP001515) NH3-dependent NAD synthetase [Bacillus halodurans]
           Length = 272
           
 Score =  102 bits (253), Expect = 3e-21
 Identities = 76/259 (29%), Positives = 125/259 (47%), Gaps = 31/259 (11%)

Query: 5   DYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKA---LGREK------VLGLI 55
           +  K +  + ++++   + G V+G+SGG DS     LA  A   L  E+       + + 
Sbjct: 20  EIRKRVTFLKNYLKHSGAKGYVLGLSGGQDSTLAGKLAQMAIDELNEEEQDTSYVFIAVR 79

Query: 56  MPYYENQDVEDAKLVVESLGIEYRV-INIRPIVDTIVSEI----GIELDKKSLGNVMART 110
           +PY   +D  DA+  +  +     + +NI+  VD          G  L   + GN  AR 
Sbjct: 80  LPYGVQKDEADAQDAIAFIKPSRSITVNIKDAVDASTKSFEQATGEVLSDFNKGNTKARE 139

Query: 111 RMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGV 170
           RM   Y        +V+GT + +E +TG+FTK GDGA D APL  L K +   + K +G 
Sbjct: 140 RMKAQYDVGAHYGCLVIGTDHAAEAITGFFTKHGDGACDVAPLFGLTKRQGKSLLKELGA 199

Query: 171 PERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEK 227
           P  +  K PTA L +   G  DE+ LG++Y+ LD+ L        E +++ + +      
Sbjct: 200 PTHLYTKAPTADLEDDRPGLPDEEALGLTYEQLDDYL--------EGKQVHDAIR----- 246

Query: 228 VEHVENLVKGSEHKRRLPI 246
            + +E+    +EHKR+LP+
Sbjct: 247 -KKIESRYLATEHKRQLPV 264


>sp|P57271|NADE_BUCAI NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|10038856|dbj|BAB12891.1|
           (AP001118) nh(3)-dependent NAD(+) synthetase [Buchnera
           sp. APS]
           Length = 268
           
 Score =  100 bits (246), Expect = 2e-20
 Identities = 78/263 (29%), Positives = 127/263 (47%), Gaps = 39/263 (14%)

Query: 7   EKAIERIIDFIREKCSNGV-----VVGISGGVDS---ATVTYLATKALGREK------VL 52
           E  I+  +DF+++   N V     +VG+SGG DS   A +  +  + L +EK       +
Sbjct: 20  EIEIKNCVDFLKKYLVNHVHIKSLIVGVSGGQDSTLTAKLCQMTAETLRKEKNDITYQFI 79

Query: 53  GLIMPY---YENQDVEDAKLVVESLGIEYRVINIRPIV---DTIVSEIGIELDKKSLGNV 106
            L +PY   Y+ +D +DA   ++   I    +NI+  V   +  + + G+ +     GN 
Sbjct: 80  ALRLPYGIQYDEKDCQDAIRFIQPDQIFN--VNIKKAVLSSEKSLKKSGVIISDYVRGNE 137

Query: 107 MARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK 166
            AR RM + Y+ A     +V+GT + +E +TG+FTK+GD  +D  P+  L K ++  + K
Sbjct: 138 KARERMKVQYSIAAMKQGLVVGTGHAAENITGFFTKYGDSGTDINPIAKLNKRQIRLLLK 197

Query: 167 RIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGI 223
            +  P+ +  KKP A L +    Q DE  LG++Y  +D  L              E   I
Sbjct: 198 NLNCPKHLYLKKPMADLEDEHPQQDDESVLGVTYDAIDSYL--------------EQKKI 243

Query: 224 PIEKVEHVENLVKGSEHKRRLPI 246
            I   + +E L   + HKR LPI
Sbjct: 244 DIRSQKIIEALYLNTLHKRNLPI 266


>pir||E82888 NH(3)-dependent NAD(+) synthetase UU460 [imported] - Ureaplasma
           urealyticum >gi|6899453|gb|AAF30872.1|AE002142_6
           (AE002142) NH(3)-dependent NAD(+) synthetase [Ureaplasma
           urealyticum]
           Length = 255
           
 Score = 95.2 bits (233), Expect = 7e-19
 Identities = 68/209 (32%), Positives = 109/209 (51%), Gaps = 14/209 (6%)

Query: 14  IDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQDVEDAKLVVES 73
           I F+ +  +N V++GISGG+DS T+T      L +E  L +   Y    D+ ++ L  E 
Sbjct: 17  IKFLNDAQTNNVILGISGGIDS-TLTLAILNDLKKEYDLNI---YAYFLDIHNSSLDYEC 72

Query: 74  LGI------EYRVINIRPIVDTIVSEIGIEL-DKKSLGNVMARTRMMILYAHANSMNRMV 126
           +           VIN+  I D+    I  +  DK  L N+  + R   LYA AN+   +V
Sbjct: 73  INELKNIYPNIEVINLVDIYDSYCKMINKKTNDKYVLYNLKPKIRTNYLYAMANAYKGVV 132

Query: 127 LGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLW-- 184
           +   N  E++ G+FTK+GD A+DY  LI L K  ++E+     +P +I+ + PT      
Sbjct: 133 VSNLNYDEYILGFFTKYGDSAADYYMLIGLLKKHIYELGAYYHLPNKILNRAPTPANEDD 192

Query: 185 EGQTDEDELGISYKLLDE-ILWRLVDLKM 212
           E +TDE   G +Y  LD+ +L+R ++ K+
Sbjct: 193 EHKTDESFFGFTYNDLDQFLLYRKINPKI 221


>gb|AAG27700.1|AF299296_3 (AF299296) NH(3)-dependent NAD(+)synthetase [Pseudomonas putida]
           Length = 275
           
 Score = 90.1 bits (220), Expect = 2e-17
 Identities = 76/231 (32%), Positives = 110/231 (46%), Gaps = 39/231 (16%)

Query: 4   LDYEKAIERIIDFIREKCSNG----VVVGISGGVDSATVTYLATKALG--REKV------ 51
           L+ EKA  R + FI++   N     +V+GISGGVDS T   LA +A+   RE+       
Sbjct: 24  LEAEKA--RRVAFIQQCLKNAGLKTLVLGISGGVDSLTGALLAQRAINELREQTGDVHYR 81

Query: 52  -LGLIMPYYENQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLG------ 104
            + + +PY    D  DA  V       +R    R +        G     +  G      
Sbjct: 82  FIAVRLPYQVQHDEHDAHGV-------HRFHPARRMTYGEHRAGGAGAGGRGRGAQGWQA 134

Query: 105 --------NVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINL 156
                   N  AR RM+  Y  A +   +V+ T + +E + G+FTK+GDGA D APL  L
Sbjct: 135 GAGGFRGGNTKARMRMVAQYTIAGARGGLVIDTDHAAEAVMGFFTKFGDGACDLAPLSGL 194

Query: 157 YKTEVWEIAKRIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEIL 204
            K +V +IA+  G PE +V+K PTA L +   G+ DE   G++Y  +D  L
Sbjct: 195 VKHQVRDIARSFGAPESLVEKVPTADLEDLAPGKPDEASHGVTYAQIDAFL 245


>pir||A70165 NH(3)-dependent NAD+ synthetase homolog - Lyme disease spirochete
           >gi|2688436|gb|AAC66885.1| (AE001154) NH(3)-dependent
           NAD+ synthetase [Borrelia burgdorferi]
           Length = 222
           
 Score = 75.3 bits (182), Expect = 7e-13
 Identities = 60/191 (31%), Positives = 101/191 (52%), Gaps = 16/191 (8%)

Query: 64  VEDAKLVVESLGIEYRVINIRPI--VDTIVSEIGIELDKKSLGNVMARTRMMILYAHANS 121
           + DAK +   LG +   + I+ +  V +   E   ++   +  N+ AR R ++L +++NS
Sbjct: 11  ISDAKELSRKLGFKLIEMPIKDLFQVSSRFFEGYFDIKGVTGENLQARLRGLLLMSYSNS 70

Query: 122 MNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIG-------VPERI 174
            N ++L T N+SE   GY T +GD     A + +L+KTEV+++   I        +P  I
Sbjct: 71  QNSLLLNTGNKSEIAVGYCTLYGDTCGGLALIGDLFKTEVYDLVNYINAKFDQCIIPVNI 130

Query: 175 VKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEK--VEHVE 232
           + K+P+A L   Q D D L   Y++LD IL R +   +  E + + + +  EK  V+ V 
Sbjct: 131 ILKEPSAELRFDQKDSDYLP-KYEVLDVILNRYL---IGNESV-DSIYLNFEKGIVDKVL 185

Query: 233 NLVKGSEHKRR 243
           NL   +E+KRR
Sbjct: 186 NLYFKNEYKRR 196


  Database: ./suso.pep
    Posted date:  Jul 6, 2001  5:57 PM
  Number of letters in database: 840,471
  Number of sequences in database:  2977
  
  Database: /banques/blast2/nr.pep
    Posted date:  Dec 14, 2000 12:46 PM
  Number of letters in database: 188,266,275
  Number of sequences in database:  595,510
  
Lambda     K      H
   0.317    0.139    0.396 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94865248
Number of Sequences: 2977
Number of extensions: 3939638
Number of successful extensions: 11276
Number of sequences better than 1.0e-10: 40
Number of HSP's better than  0.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 11163
Number of HSP's gapped (non-prelim): 42
length of query: 257
length of database: 189,106,746
effective HSP length: 56
effective length of query: 201
effective length of database: 155,591,474
effective search space: 31273886274
effective search space used: 31273886274
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 164 (68.3 bits)