BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2244 (nadE) DE:NH(3)-dependent NAD+ synthetase (nadE)
(257 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75205 nh(3)-dependent nad+ synthetase (nade) PAB2244 - Pyr... 515 e-145
sp|O57921|NADE_PYRHO PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE ... 431 e-120
pir||E69068 NH(3)-dependent NAD+ synthetase - Methanobacterium t... 243 2e-63
sp|O27554|NADE_METTH PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE 243 2e-63
gi|11498605 NH(3)-dependent NAD+ synthetase (nadE) [Archaeoglobu... 228 4e-59
sp|Q58747|NADE_METJA PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE ... 223 1e-57
emb|CAC12028.1| (AL445065) NAD(+) synthase related protein [Ther... 203 1e-51
sp|Q9YAI1|NADE_AERPE PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE 195 5e-49
pir||H72497 probable NH(3)-dependent NAD(+) synthetase APE1958 -... 187 1e-46
gb|AAK42347.1| NH(3+) dependent NAD(+) synthetase (nadE) [Sulfol... 182 3e-45
gb|AAG20193.1| (AE005096) NAD+ synthetase; NadE [Halobacterium s... 178 7e-44
sp|P47623|NADE_MYCGE PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE ... 166 2e-40
gb|AAF22217.1|AF140575_2 (AF140575) NH(3)-dependent NAD+ synthet... 164 7e-40
pir||B81353 probable NAD+ synthase (glutamine-hydrolyzing) (EC 6... 162 5e-39
sp|P75216|NADE_MYCPN PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE ... 156 2e-37
sp|O67091|NADE_AQUAE PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHET... 154 8e-37
pir||S48586 hypothetical protein - Mycoplasma capricolum (SGC3) ... 151 9e-36
sp|Q9WZB3|NAE1_THEMA NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|74489... 150 2e-35
sp|Q9X0Y0|NAE2_THEMA PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHET... 137 1e-31
sp|O25096|NADE_HELPY NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|74489... 128 8e-29
sp|P08164|NADE_BACSU NH(3)-DEPENDENT NAD(+) SYNTHETASE (SPORE OU... 122 4e-27
pdb|2NSY|A Chain A, Crystal Structure Of Nh3-Dependent Nad+ Synt... 122 4e-27
sp|P74292|NADE_SYNY3 PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHET... 122 6e-27
emb|CAB58266.1| (AL121849) NH(3)-dependent NAD(+)synthetase (EC ... 121 1e-26
sp|Q9ZMB0|NADE_HELPJ NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|74489... 120 2e-26
pir||T51756 NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) [... 120 2e-26
pir||C82617 NH3-dependent NAD synthetase XF1961 [imported] - Xyl... 120 2e-26
pir||H82488 NH(3)-dependent NAD(+) synthetase VCA0207 [imported]... 119 3e-26
pir||F83030 NH3-dependent NAD synthetase PA4920 [imported] - Pse... 119 4e-26
pir||A75617 NH3-dependent NAD+ synthase - Deinococcus radioduran... 117 1e-25
pir||C81850 NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) N... 117 1e-25
sp|Q03638|NADE_RHOCA GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(... 114 1e-24
pir||A81090 NH(3)-dependent NAD+ synthetase NadE, probable NMB13... 112 4e-24
sp|P18843|NADE_ECOLI NH(3)-DEPENDENT NAD(+) SYNTHETASE (NITROGEN... 109 3e-23
pir||A26928 nitrogen-regulatory protein - Escherichia coli >gi|1... 104 1e-21
dbj|BAB06004.1| (AP001515) NH3-dependent NAD synthetase [Bacillu... 102 3e-21
sp|P57271|NADE_BUCAI NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|10038... 100 2e-20
pir||E82888 NH(3)-dependent NAD(+) synthetase UU460 [imported] -... 95 7e-19
gb|AAG27700.1|AF299296_3 (AF299296) NH(3)-dependent NAD(+)synthe... 90 2e-17
pir||A70165 NH(3)-dependent NAD+ synthetase homolog - Lyme disea... 75 7e-13
>pir||B75205 nh(3)-dependent nad+ synthetase (nade) PAB2244 - Pyrococcus abyssi
(strain Orsay) >gi|5457598|emb|CAB49089.1| (AJ248283)
NH(3)-dependent NAD+ synthetase (nadE) [Pyrococcus
abyssi]
Length = 257
Score = 515 bits (1312), Expect = e-145
Identities = 257/257 (100%), Positives = 257/257 (100%)
Query: 1 MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE
Sbjct: 1 MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
Query: 61 NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHAN 120
NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHAN
Sbjct: 61 NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHAN 120
Query: 121 SMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPT 180
SMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPT
Sbjct: 121 SMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPT 180
Query: 181 AGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEH 240
AGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEH
Sbjct: 181 AGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEH 240
Query: 241 KRRLPIGPSFDDLIAGP 257
KRRLPIGPSFDDLIAGP
Sbjct: 241 KRRLPIGPSFDDLIAGP 257
>sp|O57921|NADE_PYRHO PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|7448970|pir||D71240
probable NH(3)-dependent NAD(+) synthetase - Pyrococcus
horikoshii >gi|3256568|dbj|BAA29251.1| (AP000001) 257aa
long hypothetical NH(3)-dependent NAD(+) synthetase
[Pyrococcus horikoshii]
Length = 257
Score = 431 bits (1097), Expect = e-120
Identities = 205/257 (79%), Positives = 235/257 (90%)
Query: 1 MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
MR LDY+K IERI++FIREK +NGVV+GISGGVDSATV YLATKALG+EKVLGLIMPY+E
Sbjct: 1 MRILDYDKVIERILEFIREKGNNGVVIGISGGVDSATVAYLATKALGKEKVLGLIMPYFE 60
Query: 61 NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHAN 120
N+DVEDAKLV E LGI Y+VINI+PIVD+ V + + LD+K LGN+M+RTRM++LYAHAN
Sbjct: 61 NKDVEDAKLVAEKLGIGYKVINIKPIVDSFVENLELNLDRKGLGNIMSRTRMIMLYAHAN 120
Query: 121 SMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPT 180
S+ R+VLGTSNRSEFLTGYFTKWGDGASDYAP+INLYKTEVWEIAKRIGVPERIVKKKP+
Sbjct: 121 SLGRIVLGTSNRSEFLTGYFTKWGDGASDYAPIINLYKTEVWEIAKRIGVPERIVKKKPS 180
Query: 181 AGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEH 240
AGLWEGQTDEDELGISY LLDEILWR++DLK+ KE+IA+DLGIP+ VE VE L+K SEH
Sbjct: 181 AGLWEGQTDEDELGISYNLLDEILWRMIDLKIGKEEIAKDLGIPLSLVERVEELIKKSEH 240
Query: 241 KRRLPIGPSFDDLIAGP 257
KRRLPIGPSF+DLI GP
Sbjct: 241 KRRLPIGPSFEDLIVGP 257
>pir||E69068 NH(3)-dependent NAD+ synthetase - Methanobacterium
thermoautotrophicum (strain Delta H)
>gi|2622628|gb|AAB85985.1| (AE000911) NH(3)-dependent
NAD+ synthetase [Methanobacterium thermoautotrophicum]
Length = 274
Score = 243 bits (613), Expect = 2e-63
Identities = 125/247 (50%), Positives = 169/247 (67%), Gaps = 6/247 (2%)
Query: 8 KAIERIIDFIREKCS----NGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYEN-- 61
+ + I DFIR+K + +GVV+G+SGGVDS+TV YLA ALG ++VLGLIMP
Sbjct: 18 EVVSVIEDFIRQKVAESGASGVVLGLSGGVDSSTVAYLAVNALGPDRVLGLIMPSSTTPR 77
Query: 62 QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANS 121
D+ A+ V + LGIE I+I PI++++ ++ +L N+ R RM+ILY HANS
Sbjct: 78 DDLRHARTVADELGIESETIDIDPIIESLTGLCSHNANELALANLKPRARMVILYYHANS 137
Query: 122 MNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTA 181
+NR+V GT NR+E L GYFTK+GDG D P+ LYK +V E+A R+GVP I+KK PTA
Sbjct: 138 LNRLVAGTGNRTELLLGYFTKYGDGGVDMLPIGGLYKGQVRELAGRLGVPPEIIKKPPTA 197
Query: 182 GLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHK 241
GLW GQTDE+ELG+ Y LLDE+L LVD K+ E++A L +P +VE + ++VKGSEHK
Sbjct: 198 GLWHGQTDEEELGMKYDLLDELLCLLVDRKLPVEEVASTLSLPPSEVERIASMVKGSEHK 257
Query: 242 RRLPIGP 248
+ P P
Sbjct: 258 LKPPEVP 264
>sp|O27554|NADE_METTH PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE
Length = 266
Score = 243 bits (613), Expect = 2e-63
Identities = 125/247 (50%), Positives = 169/247 (67%), Gaps = 6/247 (2%)
Query: 8 KAIERIIDFIREKCS----NGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYEN-- 61
+ + I DFIR+K + +GVV+G+SGGVDS+TV YLA ALG ++VLGLIMP
Sbjct: 10 EVVSVIEDFIRQKVAESGASGVVLGLSGGVDSSTVAYLAVNALGPDRVLGLIMPSSTTPR 69
Query: 62 QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANS 121
D+ A+ V + LGIE I+I PI++++ ++ +L N+ R RM+ILY HANS
Sbjct: 70 DDLRHARTVADELGIESETIDIDPIIESLTGLCSHNANELALANLKPRARMVILYYHANS 129
Query: 122 MNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTA 181
+NR+V GT NR+E L GYFTK+GDG D P+ LYK +V E+A R+GVP I+KK PTA
Sbjct: 130 LNRLVAGTGNRTELLLGYFTKYGDGGVDMLPIGGLYKGQVRELAGRLGVPPEIIKKPPTA 189
Query: 182 GLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHK 241
GLW GQTDE+ELG+ Y LLDE+L LVD K+ E++A L +P +VE + ++VKGSEHK
Sbjct: 190 GLWHGQTDEEELGMKYDLLDELLCLLVDRKLPVEEVASTLSLPPSEVERIASMVKGSEHK 249
Query: 242 RRLPIGP 248
+ P P
Sbjct: 250 LKPPEVP 256
>gi|11498605 NH(3)-dependent NAD+ synthetase (nadE) [Archaeoglobus fulgidus]
>gi|6093466|sp|O29262|NADE_ARCFU PROBABLE
NH(3)-DEPENDENT NAD(+) SYNTHETASE
>gi|7448971|pir||H69374 NH(3)-dependent NAD+ synthetase
(nadE) homolog - Archaeoglobus fulgidus
>gi|2649596|gb|AAB90242.1| (AE001035) NH(3)-dependent
NAD+ synthetase (nadE) [Archaeoglobus fulgidus]
Length = 247
Score = 228 bits (576), Expect = 4e-59
Identities = 130/258 (50%), Positives = 170/258 (65%), Gaps = 18/258 (6%)
Query: 4 LDYEKAIERIIDFIR----EKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP-- 57
+++EK +ERI DFIR S GVV+G+SGGVDSATV YL +ALG E+V LIMP
Sbjct: 1 MNFEKVVERICDFIRGVVSSSGSTGVVLGLSGGVDSATVAYLCVRALGSERVFALIMPET 60
Query: 58 -YYENQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILY 116
QDVEDA V ESLG+EY++I I IV + G E K + N+ R RM++ Y
Sbjct: 61 GVTPEQDVEDAINVAESLGMEYKLIEINDIVRVFKEKAG-EGSKIAEANLKPRIRMVLNY 119
Query: 117 AHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVK 176
HANSMNR+V GT N+SE + GYFTK+GDG D+ P+ +LYKTEV+++A +GVP RI++
Sbjct: 120 YHANSMNRLVAGTGNKSELMVGYFTKYGDGGVDFLPIGDLYKTEVFQLAAYLGVPRRIIE 179
Query: 177 KKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVK 236
KKP+A LW GQTDE+E+GISY LDEIL +L+ EK + +D EK V +V+
Sbjct: 180 KKPSARLWPGQTDEEEMGISYAELDEIL-KLI----EKGERRDD-----EKFRRVVQMVE 229
Query: 237 GSEHKRRLPIGPSFDDLI 254
S HKR +P DL+
Sbjct: 230 RSRHKREMPPVARVRDLL 247
>sp|Q58747|NADE_METJA PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|2129159|pir||G64468
NH(3)-dependent NAD+ synthetase - Methanococcus
jannaschii >gi|1591995|gb|AAB99363.1| (U67575)
NH(3)-dependent NAD+ synthetase (nadE) [Methanococcus
jannaschii]
Length = 259
Score = 223 bits (563), Expect = 1e-57
Identities = 125/251 (49%), Positives = 168/251 (66%), Gaps = 10/251 (3%)
Query: 7 EKAIERIIDFIREKC----SNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYEN- 61
E+ + +I FIREK +NGVVVG+SGG+DS+ YL KALG++KVLGLIMP
Sbjct: 8 EEIVNKITKFIREKVEEANANGVVVGLSGGIDSSVTAYLCVKALGKDKVLGLIMPEKNTN 67
Query: 62 -QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGI---ELDKKSLGNVMARTRMMILYA 117
+DVE AK+V E+LGI+Y + +I I+ + + E DK + GN+ AR RM ILY
Sbjct: 68 PKDVEHAKMVAENLGIKYIISDITDILKAFGAGGYVPTREFDKIADGNLKARIRMCILYY 127
Query: 118 HANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKK 177
AN N +V GTSN+SE GY TK GD A D P+ NL+KTEV ++AK IGVP+ I++K
Sbjct: 128 FANKYNLLVAGTSNKSEIYVGYGTKHGDIACDIRPIGNLFKTEVKKLAKYIGVPKEIIEK 187
Query: 178 KPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKG 237
P+AGLWEGQTDE+EL I Y+ LD IL +L + E+I ++ IP+E + +V +L+K
Sbjct: 188 PPSAGLWEGQTDEEELDIKYETLDTIL-KLYEKGKTPEEIHKETNIPLETINYVFDLIKK 246
Query: 238 SEHKRRLPIGP 248
+EHKR LP P
Sbjct: 247 NEHKRTLPPTP 257
>emb|CAC12028.1| (AL445065) NAD(+) synthase related protein [Thermoplasma
acidophilum]
Length = 241
Score = 203 bits (512), Expect = 1e-51
Identities = 107/244 (43%), Positives = 160/244 (64%), Gaps = 13/244 (5%)
Query: 5 DYEKAIERIIDFIREKCSN-GVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ- 62
DY + IERI +F+R+ V+GISGG+DS+ L T+++ +E+++ + MP Q
Sbjct: 3 DYMQEIERISEFLRKVLQGKNAVIGISGGIDSSVTLALLTRSIPKERIIPVFMPDRNTQQ 62
Query: 63 -DVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANS 121
D +D + + + LG+ YR + I P+VD+ VS + D+ ++GN+ +RTRM+ILY AN+
Sbjct: 63 ADYDDVQKLTQKLGLTYREVRIDPMVDSFVSTLAAT-DRAAIGNIKSRTRMIILYYFANT 121
Query: 122 MNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTA 181
MV+GT+NR+E L GYFTK+GDG D P+ +LYK +V+E+AK + +PE I+KKKPTA
Sbjct: 122 NGGMVVGTTNRTELLLGYFTKYGDGGCDVEPIEHLYKRDVYELAKILDIPESIMKKKPTA 181
Query: 182 GLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHK 241
GLW GQTDEDE+G+SY +DEIL + D +K +K+ + +L S+HK
Sbjct: 182 GLWAGQTDEDEIGMSYAQMDEILSDVFDKGTYNDK------PEYKKIMEMHSL---SDHK 232
Query: 242 RRLP 245
R LP
Sbjct: 233 RNLP 236
>sp|Q9YAI1|NADE_AERPE PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE
Length = 280
Score = 195 bits (490), Expect = 5e-49
Identities = 113/261 (43%), Positives = 156/261 (59%), Gaps = 11/261 (4%)
Query: 4 LDYEKAIERIIDFIREKC----SNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYY 59
+DY + I F+R+ ++G V+G+SGGVDS+ LA A+G +V LIMP
Sbjct: 9 IDYNGVRQAITQFLRKYLEASGASGYVLGVSGGVDSSLALALAVDAVGSGRVTALIMPDR 68
Query: 60 E---NQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGI----ELDKKSLGNVMARTRM 112
E +DVEDA +V S G+E+ VI+I PIV +S + I E D+ +GN+ AR R
Sbjct: 69 EVTPERDVEDALRLVRSFGVEHAVIDISPIVMVYISALPIFEDEEKDRVPVGNLRARIRA 128
Query: 113 MILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPE 172
ILY +AN + ++VLGT +RSE+L GYFTK+GD A D APL LYK++V +A+ IGVP
Sbjct: 129 NILYYYANKLGKLVLGTGDRSEYLIGYFTKYGDAACDVAPLTVLYKSQVRRLAELIGVPR 188
Query: 173 RIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVE 232
I K + LW+G E ELG+SY +D IL+ DLK+ E+I G+ KVE V
Sbjct: 189 DIAYKPSSPRLWKGHEAEAELGLSYNEIDVILYSRFDLKIPWEEIPRATGLERAKVERVR 248
Query: 233 NLVKGSEHKRRLPIGPSFDDL 253
L + S HKR P P ++
Sbjct: 249 LLHEASSHKRSPPASPDLGEI 269
>pir||H72497 probable NH(3)-dependent NAD(+) synthetase APE1958 - Aeropyrum
pernix (strain K1) >gi|5105656|dbj|BAA80968.1|
(AP000063) 246aa long hypothetical NH(3)-dependent
NAD(+) synthetase [Aeropyrum pernix]
Length = 246
Score = 187 bits (470), Expect = 1e-46
Identities = 106/235 (45%), Positives = 144/235 (61%), Gaps = 7/235 (2%)
Query: 26 VVGISGGVDSATVTYLATKALGREKVLGLIMPYYE---NQDVEDAKLVVESLGIEYRVIN 82
++G+SGGVDS+ LA A+G +V LIMP E +DVEDA +V S G+E+ VI+
Sbjct: 1 MLGVSGGVDSSLALALAVDAVGSGRVTALIMPDREVTPERDVEDALRLVRSFGVEHAVID 60
Query: 83 IRPIVDTIVSEIGI----ELDKKSLGNVMARTRMMILYAHANSMNRMVLGTSNRSEFLTG 138
I PIV +S + I E D+ +GN+ AR R ILY +AN + ++VLGT +RSE+L G
Sbjct: 61 ISPIVMVYISALPIFEDEEKDRVPVGNLRARIRANILYYYANKLGKLVLGTGDRSEYLIG 120
Query: 139 YFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQTDEDELGISYK 198
YFTK+GD A D APL LYK++V +A+ IGVP I K + LW+G E ELG+SY
Sbjct: 121 YFTKYGDAACDVAPLTVLYKSQVRRLAELIGVPRDIAYKPSSPRLWKGHEAEAELGLSYN 180
Query: 199 LLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRRLPIGPSFDDL 253
+D IL+ DLK+ E+I G+ KVE V L + S HKR P P ++
Sbjct: 181 EIDVILYSRFDLKIPWEEIPRATGLERAKVERVRLLHEASSHKRSPPASPDLGEI 235
>gb|AAK42347.1| NH(3+) dependent NAD(+) synthetase (nadE) [Sulfolobus solfataricus]
Length = 304
Score = 182 bits (458), Expect = 3e-45
Identities = 101/238 (42%), Positives = 151/238 (63%), Gaps = 3/238 (1%)
Query: 10 IERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQDVEDAKL 69
+ERI +++ G V+G+SGGVDSA L KA +L + D++D+
Sbjct: 47 VERIREYLEFSNKKGGVIGVSGGVDSAVTATLLAKATDNFFILLMPSSSTPKIDLDDSFE 106
Query: 70 VVESLGIE--YRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANSMNRMVL 127
+++ L + Y++INI IV + ++I E +K +GN+ AR RM+ILYA+A ++ +V+
Sbjct: 107 MIKFLNAQNKYKLINIDEIVKSFSNKIETE-NKYIIGNIKARVRMIILYAYAQMLDYLVV 165
Query: 128 GTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQ 187
GT ++SE L GYFTK+GDG D P+ +LYKT+V + K +G+PERIV K + LWEGQ
Sbjct: 166 GTGDKSELLLGYFTKYGDGGVDVLPIGDLYKTQVRMLGKCLGLPERIVTKPSSPALWEGQ 225
Query: 188 TDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRRLP 245
T E ELGI Y+ +D IL+ D +++I + LGIPI+ V+ V+ LVK S+HKR P
Sbjct: 226 TAEGELGIDYETIDSILYLRFDEMRSEDEIVKMLGIPIDIVKKVDRLVKISQHKRLPP 283
>gb|AAG20193.1| (AE005096) NAD+ synthetase; NadE [Halobacterium sp. NRC-1]
Length = 268
Score = 178 bits (446), Expect = 7e-44
Identities = 104/245 (42%), Positives = 144/245 (58%), Gaps = 7/245 (2%)
Query: 11 ERIIDFIREKCSNG----VVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQDV 64
+RI FIR+ + V+G+SGG+DS TV +L LG + + GL+MP +Q++
Sbjct: 24 DRIQSFIRDTVAAAGAERCVLGLSGGIDSTTVAHLTVDELGADALHGLVMPGAVSRDQNM 83
Query: 65 EDAKLVVESLGIEYRVINIRPIVDTIVSEIGIEL-DKKSLGNVMARTRMMILYAHANSMN 123
DA+ V E LGIEY V+ I P V + D+ ++GN ARTR +I Y AN +
Sbjct: 84 SDAERVAEDLGIEYDVVEIDPFVTQLTDVFPDAAGDEVAVGNARARTRAVINYFVANHGD 143
Query: 124 RMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGL 183
+VLGT NR+E +TGY+TK+GD A D P+ NLYK +V ++A+ +GVPE +V K PTA L
Sbjct: 144 GVVLGTGNRAEAMTGYYTKYGDQAVDCNPIGNLYKMQVRQLARDLGVPEDLVTKAPTAEL 203
Query: 184 WEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRR 243
W QTD ELG+ Y +D +L VD + A L I VE V +L S+HKR
Sbjct: 204 WADQTDAGELGVDYDTIDAVLAVHVDGGLPASATATHLDIDPSVVETVRDLYGASKHKRA 263
Query: 244 LPIGP 248
+P P
Sbjct: 264 MPPAP 268
>sp|P47623|NADE_MYCGE PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|1361857|pir||D64242
sporulation protein outB homolog - Mycoplasma genitalium
>gi|3844972|gb|AAC71610.1| (U39720) NH(3)-dependent NAD+
synthetase, putative [Mycoplasma genitalium]
Length = 248
Score = 166 bits (416), Expect = 2e-40
Identities = 95/234 (40%), Positives = 139/234 (58%), Gaps = 7/234 (2%)
Query: 4 LDYEKAIER-IIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ 62
+ Y K ++ + D++++ + GV+ G+SGG+DSA V +A + G E L LIM ++
Sbjct: 5 IKYLKELQNWLFDYVKKSKAKGVIFGLSGGIDSAVVAAIAKETFGFENHLALIMHINNSK 64
Query: 63 -DVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSL--GNVMARTRMMILYAHA 119
D + +V+ + I + + +V +GI+ K L GN+ AR RM+ LYA+A
Sbjct: 65 LDFQATSELVKKMQFNSINIELEESFNLLVKTLGIDPKKDFLTAGNIKARLRMITLYAYA 124
Query: 120 NSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKP 179
N +VLGT N E+ GYFTKWGDGA D APL L K +V+++AK +PE ++ + P
Sbjct: 125 QKHNFLVLGTGNFVEYTLGYFTKWGDGACDIAPLAWLLKEDVYKLAKHFNIPEIVITRAP 184
Query: 180 TAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVEN 233
TA L+EGQTDE E+GI+YK LD+ L + L EK+KI DL K EH N
Sbjct: 185 TASLFEGQTDETEMGITYKELDQYLKGDLILSSEKQKIVLDLK---AKAEHKHN 235
>gb|AAF22217.1|AF140575_2 (AF140575) NH(3)-dependent NAD+ synthetase [Mycoplasma
hyopneumoniae]
Length = 246
Score = 164 bits (412), Expect = 7e-40
Identities = 97/235 (41%), Positives = 138/235 (58%), Gaps = 18/235 (7%)
Query: 10 IERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYEN-QDVEDAK 68
IE I +++ GV+ G+SGGVDSA V LA KA + LGLIMP + D+ D
Sbjct: 15 IEWIRQEVKKAQKKGVIFGLSGGVDSALVAVLANKAFP-DSHLGLIMPIRDMITDMVDID 73
Query: 69 LVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANSMNRMVLG 128
L+V+ GI+ + IN++P + + ++ +K + N+ R RM LYA A + +VLG
Sbjct: 74 LLVKKFGIKNKEINLKPAFENLKKCFNLK-NKLANSNIQPRLRMTSLYAFAQEFDYLVLG 132
Query: 129 TSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQT 188
T N SE GYFTK+GDG D P++NL K +VW++A IG+PE I+KKKP+A LWE QT
Sbjct: 133 TDNFSEMYLGYFTKYGDGGVDLLPIVNLTKVQVWKMAAEIGIPESIIKKKPSANLWENQT 192
Query: 189 DEDELGISYKLLDEILWRLVDLKMEKEK-IAEDLGIPIEKVEHVENLVKGSEHKR 242
DE+E+G SY L DL ME +++++ I K+ + S HKR
Sbjct: 193 DENEMGFSYGDL--------DLFMENPNLVSKEIAAKITKLHEI------SSHKR 233
>pir||B81353 probable NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) Cj0810
[similarity] - Campylobacter jejuni (strain NCTC 11168)
>gi|6968256|emb|CAB73075.1| (AL139076) putative
NH(3)-dependent NAD(+) synthetase [Campylobacter jejuni]
Length = 246
Score = 162 bits (405), Expect = 5e-39
Identities = 95/248 (38%), Positives = 149/248 (59%), Gaps = 13/248 (5%)
Query: 4 LDYEKAIERIIDFIREKCSN----GVVVGISGGVDSATVTYLATKALGREKVLGLIMPYY 59
+D++K E++ DFI+EK N GVV+G+SGG+DSA V L +AL +E V L+MP
Sbjct: 1 MDWQKITEKMCDFIQEKVKNSQSQGVVLGLSGGIDSALVATLCKRAL-KENVFALLMPTQ 59
Query: 60 --ENQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYA 117
++EDA + L +EY++I I+ I+D + + SLGN AR RM +LY
Sbjct: 60 ISNKANLEDALRLCADLNLEYKIIEIQSILDAFIKQ-SENTTLVSLGNFAARIRMSLLYD 118
Query: 118 HANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKK 177
++ N +V+GTSN+SE L GY T +GD A + P+ +LYK+E++ +AK + + E +KK
Sbjct: 119 YSALKNSLVIGTSNKSELLLGYGTIYGDLACAFNPIGSLYKSEIYALAKYLNLHENFIKK 178
Query: 178 KPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKG 237
P+A LWE Q+DE +LG SY +DE L L+ EK+ L + + ++N ++
Sbjct: 179 APSADLWENQSDEADLGFSYTKIDE---GLKALETNDEKLLRTLDPSL--IAMLKNRMQK 233
Query: 238 SEHKRRLP 245
+ K ++P
Sbjct: 234 NAFKGKMP 241
>sp|P75216|NADE_MYCPN PROBABLE NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|2146516|pir||S73606
probable NH(3)-dependent NAD(+) synthetase outB -
Mycoplasma pneumoniae (strain ATCC 29342)
>gi|1673951|gb|AAB95928.1| (AE000027) probable
NH(3)-dependent NAD(+) synthetase [Mycoplasma
pneumoniae]
Length = 248
Score = 156 bits (391), Expect = 2e-37
Identities = 92/246 (37%), Positives = 143/246 (57%), Gaps = 18/246 (7%)
Query: 4 LDYEKAIER-IIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ 62
L+Y +E + +++ E + GVVVG+SGGVDSA V + TK L + L L+M ++
Sbjct: 6 LNYLNELEAWLTNYVNEAHAQGVVVGLSGGVDSAVVAAM-TKKLFPQNHLTLVMHINNSE 64
Query: 63 -DVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSL--GNVMARTRMMILYAHA 119
D + +VE L + + +++ P ++ + I+ K+ + GN+ AR RM LY HA
Sbjct: 65 LDHKATTALVEQLQLNNKQVDLEPPYRAMLQALTIDPQKELMVAGNLKARLRMACLYTHA 124
Query: 120 NSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKP 179
N +VLGT N E+ GYFTKWGDGA D APL L K++V+ +++ VPE ++++ P
Sbjct: 125 QKHNYLVLGTGNFIEYSLGYFTKWGDGACDVAPLAFLLKSDVYALSQHFNVPELVIERAP 184
Query: 180 TAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSE 239
TA L+ GQTDE E+G++YK LD+ + L K+ + V++L + S+
Sbjct: 185 TASLFAGQTDEAEMGLTYKELDQYFQGHLQLSATKQ-------------QRVDHLRQSSQ 231
Query: 240 HKRRLP 245
HKR LP
Sbjct: 232 HKRSLP 237
>sp|O67091|NADE_AQUAE PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(+) SYNTHASE
[GLUTAMINE-HYDROLYSING]) >gi|7451134|pir||H70382
NH(3)-dependent NAD+ synthetase - Aquifex aeolicus
>gi|2983464|gb|AAC07044.1| (AE000715) NH(3)-dependent
NAD+ synthetase [Aquifex aeolicus]
Length = 567
Score = 154 bits (386), Expect = 8e-37
Identities = 94/246 (38%), Positives = 146/246 (59%), Gaps = 14/246 (5%)
Query: 15 DFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQDVEDAKLVVE 72
D++R+ VV+G+SGG+DS+ V LA ALGRE V G+ MP + + EDAK + +
Sbjct: 303 DYVRKNGFEKVVLGLSGGIDSSFVACLAVDALGRENVKGVYMPSQFSSKESYEDAKALAQ 362
Query: 73 SLGIEYRVINIRPIVDTIVSEIGIELDKKSLG----NVMARTRMMILYAHANSMNRMVLG 128
+LGIE+ VI I+ I +E E+ + + N+ AR R IL+ +N +VL
Sbjct: 363 NLGIEFHVIPIKEIYRAYFNEFEKEICEITFDVADENIQARIRANILFYFSNKFRYLVLS 422
Query: 129 TSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK-----RIGVPERIVKKKPTAGL 183
TSN+SE GY T +GD A +AP+ ++YKT V+++A+ +PER+ KK P+A L
Sbjct: 423 TSNKSETAVGYTTIYGDMAGGFAPIKDVYKTWVYKLARYRNSISPDIPERVFKKPPSAEL 482
Query: 184 WEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRR 243
QTD+D L Y++LD+IL ++ + E+I G+P + V N+++ +E+KR+
Sbjct: 483 RPNQTDQDVLP-PYEILDQILMLYIEENLSPEEIIRK-GLPRDAVYKTINMIRKNEYKRK 540
Query: 244 -LPIGP 248
PIGP
Sbjct: 541 QAPIGP 546
>pir||S48586 hypothetical protein - Mycoplasma capricolum (SGC3) (fragment)
>gi|7447178|pir||S77778 probable NH(3)-dependent NAD(+)
synthetase outB - Mycoplasma capricolum (fragment)
>gi|4545064|emb|CAA83707.1| (Z33030) ATP pyrophosphatase
[Mycoplasma capricolum]
Length = 253
Score = 151 bits (377), Expect = 9e-36
Identities = 98/250 (39%), Positives = 144/250 (57%), Gaps = 29/250 (11%)
Query: 10 IERIIDFIRE-----KCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ-D 63
++ +++FI++ KC +GVVVGISGG+DSA V LA +A + L + MP Y +Q D
Sbjct: 17 LDYLVEFIQQTVKKAKC-DGVVVGISGGIDSAVVANLAKRAFP-DNYLTVWMPIYSSQLD 74
Query: 64 VEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKK----SLGNVMARTRMMILYAHA 119
+ ++++ ++ +N+ D + LD+K ++ N AR RM LY A
Sbjct: 75 YDCVNELIKTNQLKNIEVNLEASFDAFKNSFS-NLDEKPNLLAISNSKARLRMTTLYTIA 133
Query: 120 NSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKP 179
+ +VLGT N E+ GYFTK+GDG D P+I+L K+EV + AK + VPE I+ +KP
Sbjct: 134 QTKKYLVLGTDNLDEWHIGYFTKYGDGGVDVVPIIHLLKSEVKKAAKILNVPELIINRKP 193
Query: 180 TAGLWEGQTDEDELGISYKLLDE-ILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGS 238
TAGLWEGQTDE E+G SY L+D +L + D K++K ++ L K S
Sbjct: 194 TAGLWEGQTDEGEIGFSYDLIDSYLLKQNNDPKLKK---------------RIDYLHKIS 238
Query: 239 EHKRRLPIGP 248
+HKR L I P
Sbjct: 239 KHKRMLAIKP 248
>sp|Q9WZB3|NAE1_THEMA NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|7448977|pir||D72351
hypothetical protein TM0645 - Thermotoga maritima
(strain MSB8) >gi|4981167|gb|AAD35729.1|AE001738_9
(AE001738) NH(3)-dependent NAD(+) synthetase, putative
[Thermotoga maritima]
Length = 281
Score = 150 bits (374), Expect = 2e-35
Identities = 103/281 (36%), Positives = 151/281 (53%), Gaps = 52/281 (18%)
Query: 11 ERIIDFIREKCSN----GVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ--DV 64
E +I FI+EK G VVG+SGGVDSA V L +ALG+++V LI+P ++ +
Sbjct: 3 ESLISFIKEKIEEYNYRGAVVGVSGGVDSAVVLSLCVQALGKDRVFALILPERDSSKDSL 62
Query: 65 EDAKLVVESLGIEYRVINIRPIV----------------DTIVSEIGIEL---------- 98
+DA E LG+EYR +I PI+ D+IV +
Sbjct: 63 KDAVDFCERLGVEYRKRSITPILRKIGVYRLFPPRLFLPDSIVKRYVLNRWNTLSKDPFL 122
Query: 99 -DKKSLG-----------NVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDG 146
D ++ G + R RM +LY A V+GT+NR+E+LTG + KWGD
Sbjct: 123 DDLRNTGPEEFLKGLAYYRIKHRIRMCLLYFEAEKRGYAVVGTTNRTEYLTGLYVKWGDE 182
Query: 147 ASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQTDEDELGISYKLLDEILWR 206
A D P+++LYKT+V+E+AK + VPE+I+KK P+ L G TDE +SY LD IL
Sbjct: 183 AVDIEPIMHLYKTQVFELAKEMNVPEKILKKPPSPDLIPGITDEMAFNMSYLELDRIL-- 240
Query: 207 LVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSE-HKRRLPI 246
+K+EK + D +KVE V+ +++ SE ++R +PI
Sbjct: 241 ---MKLEKNEDLSD--EDPKKVERVKKILELSEKYRRDIPI 276
>sp|Q9X0Y0|NAE2_THEMA PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(+) SYNTHASE
[GLUTAMINE-HYDROLYSING]) >gi|7451135|pir||G72277
NH(3)-dependent NAD(+) synthetase - Thermotoga maritima
(strain MSB8) >gi|4981808|gb|AAD36328.1|AE001780_12
(AE001780) NH(3)-dependent NAD(+) synthetase [Thermotoga
maritima]
Length = 576
Score = 137 bits (342), Expect = 1e-31
Identities = 89/252 (35%), Positives = 144/252 (56%), Gaps = 17/252 (6%)
Query: 6 YEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQD 63
+ I + D++R+ VV+G+SGG+DS+ V +AT+ALG+E V G++MP Y +
Sbjct: 299 FRALITGLRDYVRKNGFEKVVIGLSGGMDSSLVAVIATEALGKENVKGVLMPSMYTSKES 358
Query: 64 VEDAKLVVESLGIEYRVINIRPIVDTIVSEI-----GIELDKKSLGNVMARTRMMILYAH 118
+EDA+ + ++LGIE +I I + + + + G E D N+ AR R L A
Sbjct: 359 IEDAQTLAKNLGIETFIIPITDVFHSYLETLKGVFAGREPDITE-ENLQARIRGNYLMAL 417
Query: 119 ANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIG-------VP 171
+N +VL T N+SE TGY T +GD A +A + ++YKT+V+ I + +P
Sbjct: 418 SNKFGWLVLTTGNKSEMATGYATLYGDMAGGFAVIKDVYKTDVYRIGRWYNSWRGKEIIP 477
Query: 172 ERIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHV 231
E I K PTA L GQTD+++L Y++LDEIL ++ ++ E+IA G + V V
Sbjct: 478 ENIFVKPPTAELRPGQTDQEKLP-PYEVLDEILRLYIEEGLDPEEIASK-GFDRKTVLDV 535
Query: 232 ENLVKGSEHKRR 243
+++ +E+KR+
Sbjct: 536 TEMIRKNEYKRK 547
>sp|O25096|NADE_HELPY NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|7448974|pir||A64561
NH(3)-dependent NAD+ synthetase - Helicobacter pylori
(strain 26695) >gi|2313427|gb|AAD07396.1| (AE000550)
NH(3)-dependent NAD+ synthetase (nadE) [Helicobacter
pylori 26695]
Length = 260
Score = 128 bits (318), Expect = 8e-29
Identities = 86/251 (34%), Positives = 138/251 (54%), Gaps = 13/251 (5%)
Query: 5 DYEKAIERIIDFIREKCSNG----VVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
DY+K I + DF+ ++ VV G+SGG+DSA V L K +E L+MP
Sbjct: 4 DYQKLIVYLCDFLEKEVQKRGFKKVVYGLSGGLDSAVVGVLCQKVF-KENAHALLMPSSV 62
Query: 61 N--QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAH 118
+ ++ DA + E I Y +I P D I S + GN AR RM LY +
Sbjct: 63 SMPENKTDALNLCEKFSIPYTEYSIAPY-DAIFSSHFKDASLTRKGNFCARLRMAFLYDY 121
Query: 119 ANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKK 178
+ + +V+GTSN+SE + GY T +GD A P+ L+KTEV+E+A+R+ +P++I+ K
Sbjct: 122 SLKSDSLVIGTSNKSERMLGYGTLFGDLACAINPIGELFKTEVYELARRLNIPKKILNKP 181
Query: 179 PTAGLWEGQTDEDELGISYKLLDEILWRLVDL----KMEKEKIAEDLGIPIEKVEHVENL 234
P+A L+ GQ+DE +LG Y ++D +L + L ++ E +A+ LG V+++ +
Sbjct: 182 PSADLFVGQSDEKDLGYPYSVIDPLLKDIEALFQTKPIDTETLAQ-LGYDEILVKNITSR 240
Query: 235 VKGSEHKRRLP 245
++ + K LP
Sbjct: 241 IQKNAFKLELP 251
>sp|P08164|NADE_BACSU NH(3)-DEPENDENT NAD(+) SYNTHETASE (SPORE OUTGROWTH FACTOR B)
(SPORULATION PROTEIN OUTB) (GENERAL STRESS PROTEIN 38)
(GSP38) >gi|80412|pir||A26936 NAD+ synthase (EC 6.3.1.5)
[validated] - Bacillus subtilis
>gi|143279|gb|AAA22635.1| (M15811) outB [Bacillus
subtilis] >gi|1805385|dbj|BAA08947.1| (D50453) spore
outgrowth factor B [Bacillus subtilis]
>gi|2632599|emb|CAB12107.1| (Z99105) NH3-dependent NAD+
synthetase [Bacillus subtilis]
Length = 272
Score = 122 bits (303), Expect = 4e-27
Identities = 91/269 (33%), Positives = 141/269 (51%), Gaps = 34/269 (12%)
Query: 3 TLDYEKAIERIIDFIREKC----SNGVVVGISGGVDSATVTYLATKAL-------GREKV 51
++D ++ IE ++F+++ + G V+GISGG DS LA A+ G +
Sbjct: 16 SIDPKQEIEDRVNFLKQYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVESIREEGGDAQF 75
Query: 52 LGLIMPYYENQDVEDAKLVVESLGIEYR-VINIRPIV----DTIVSEIGIELDKKSLGNV 106
+ + +P+ QD +DA+L ++ + + +I+ V D E G +L + GNV
Sbjct: 76 IAVRLPHGTQQDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNV 135
Query: 107 MARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK 166
ARTRM+ YA +VLGT + +E +TG+FTK+GDG +D PL L K + + K
Sbjct: 136 KARTRMIAQYAIGGQEGLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLK 195
Query: 167 RIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGI 223
+G PER+ K+PTA L + Q+DE ELGISY +D+ L + K K++E L
Sbjct: 196 ELGAPERLYLKEPTADLLDEKPQQSDETELGISYDEIDDYL----EGKEVSAKVSEAL-- 249
Query: 224 PIEKVEHVENLVKGSEHKRRLPIGPSFDD 252
E +EHKR++P FDD
Sbjct: 250 --------EKRYSMTEHKRQVP-ASMFDD 269
>pdb|2NSY|A Chain A, Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
Bacillus Subtilis In Complex With Nad-Adenylate
>gi|4389118|pdb|2NSY|B Chain B, Crystal Structure Of
Nh3-Dependent Nad+ Synthetase From Bacillus Subtilis In
Complex With Nad-Adenylate >gi|2392477|pdb|1NSY|A Chain
A, Crystal Structure Of Nh3-Dependent Nad+ Synthetase
From Bacillus Subtilis >gi|2392478|pdb|1NSY|B Chain B,
Crystal Structure Of Nh3-Dependent Nad+ Synthetase From
Bacillus Subtilis
Length = 271
Score = 122 bits (303), Expect = 4e-27
Identities = 91/269 (33%), Positives = 141/269 (51%), Gaps = 34/269 (12%)
Query: 3 TLDYEKAIERIIDFIREKC----SNGVVVGISGGVDSATVTYLATKAL-------GREKV 51
++D ++ IE ++F+++ + G V+GISGG DS LA A+ G +
Sbjct: 15 SIDPKQEIEDRVNFLKQYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVESIREEGGDAQF 74
Query: 52 LGLIMPYYENQDVEDAKLVVESLGIEYR-VINIRPIV----DTIVSEIGIELDKKSLGNV 106
+ + +P+ QD +DA+L ++ + + +I+ V D E G +L + GNV
Sbjct: 75 IAVRLPHGTQQDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNV 134
Query: 107 MARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK 166
ARTRM+ YA +VLGT + +E +TG+FTK+GDG +D PL L K + + K
Sbjct: 135 KARTRMIAQYAIGGQEGLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLK 194
Query: 167 RIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGI 223
+G PER+ K+PTA L + Q+DE ELGISY +D+ L + K K++E L
Sbjct: 195 ELGAPERLYLKEPTADLLDEKPQQSDETELGISYDEIDDYL----EGKEVSAKVSEAL-- 248
Query: 224 PIEKVEHVENLVKGSEHKRRLPIGPSFDD 252
E +EHKR++P FDD
Sbjct: 249 --------EKRYSMTEHKRQVP-ASMFDD 268
>sp|P74292|NADE_SYNY3 PROBABLE GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(+) SYNTHASE
[GLUTAMINE-HYDROLYSING]) >gi|7451133|pir||S75927
hypothetical protein - Synechocystis sp. (strain PCC
6803) >gi|1653472|dbj|BAA18386.1| (D90913)
NH(3)-dependent NAD(+) synthetase [Synechocystis sp.]
Length = 558
Score = 122 bits (302), Expect = 6e-27
Identities = 80/240 (33%), Positives = 130/240 (53%), Gaps = 13/240 (5%)
Query: 15 DFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQDVEDAKLVVE 72
D++ + + ++G+SGG+DS+ V +A +ALG+E VLG++MP Y + ++DA + +
Sbjct: 291 DYLHKCGFSKAILGLSGGIDSSLVAAIAVEALGKENVLGILMPSPYSSDHSIQDALALAK 350
Query: 73 SLGIEYRVINIRPIVDTIVSEIGIELDKKSLG----NVMARTRMMILYAHANSMNRMVLG 128
+LG+ + I I PI+ T + G N+ +R R +L A AN ++L
Sbjct: 351 NLGMNTQTIPIGPIMATYDQALVPLFQDAPFGLAEENLQSRIRGNLLMAIANKFGHLLLS 410
Query: 129 TSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIG-----VPERIVKKKPTAGL 183
T N+SE GY T +GD A + ++ KT+V+E+ + + +P ++ K P+A L
Sbjct: 411 TGNKSELAVGYCTLYGDMNGGLAAIADVPKTQVFELCRWLNREQTIIPPSVLTKPPSAEL 470
Query: 184 WEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRR 243
GQ D D L Y +LD IL RLV+ ++I + G E V + LV+ SE KRR
Sbjct: 471 KPGQVDTDSLP-PYDVLDGILGRLVEKHQSPQEII-NAGFEREVVLKICQLVQKSEFKRR 528
>emb|CAB58266.1| (AL121849) NH(3)-dependent NAD(+)synthetase (EC 6.3.5.1)
[Streptomyces coelicolor A3(2)]
Length = 276
Score = 121 bits (300), Expect = 1e-26
Identities = 85/270 (31%), Positives = 138/270 (50%), Gaps = 39/270 (14%)
Query: 3 TLDYEKAIERIIDFIREKCSN----GVVVGISGGVDSATVTYLATKALGREKVLG----- 53
T D E+ IER + F+ E+ ++ +V+GISGGVDS T L A+ R + G
Sbjct: 22 TFDAEREIERRVAFLAERLTSTGLRALVLGISGGVDSTTAGRLCQLAVERARAAGHEALF 81
Query: 54 --LIMPYYENQDVEDAKLVVESLGIE-YRVINIRPIVDTIVSEIGIELDKKSL------- 103
+ +P+ D +DA+ + + + ++I+P D + + + D
Sbjct: 82 YAMRLPHGVQADEDDAQQALSFIRPDRVLTVDIKPASDAALDAL-LAADVAFRDPHHQDF 140
Query: 104 --GNVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEV 161
GN+ AR RM+ YA A + +V+GT + +E ++G+FTK+GDGA+D PL L K V
Sbjct: 141 VHGNIKARQRMIAQYAVAGAHGGLVVGTDHAAEAVSGFFTKFGDGAADVVPLTGLTKRRV 200
Query: 162 WEIAKRIGVPERIVKKKPTAGLW---EGQTDEDELGISYKLLDEILWRLVDLKMEKEKIA 218
+ +G P +V+K PTA L G+ DED LG++Y+ +D+ L E + +
Sbjct: 201 RAVGDALGAPAALVRKVPTADLETLDPGKADEDALGVTYEEIDDFL--------EGKPVT 252
Query: 219 EDLGIPIEKVEHVENLVKGSEHKRRLPIGP 248
E + E + + ++HKRRLPI P
Sbjct: 253 EQV------FETIVTRYRQTDHKRRLPIAP 276
>sp|Q9ZMB0|NADE_HELPJ NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|7448973|pir||A71949
nh(3)-dependent nad+ synthetase - Helicobacter pylori
(strain J99) >gi|4154831|gb|AAD05885.1| (AE001467)
NH(3)-DEPENDENT NAD+ SYNTHETASE [Helicobacter pylori
J99]
Length = 260
Score = 120 bits (298), Expect = 2e-26
Identities = 85/250 (34%), Positives = 131/250 (52%), Gaps = 11/250 (4%)
Query: 5 DYEKAIERIIDF----IREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
DY+K I + DF +++K VV G+SGG+DSA V L K +E L+MP
Sbjct: 4 DYQKLIVYLCDFLEKEVQKKGFKKVVYGLSGGLDSAVVGVLCQKVF-KENAHALLMPSSV 62
Query: 61 N--QDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAH 118
+ + DA + E I Y +I P D I + GN AR RM LY +
Sbjct: 63 SMPESRTDALNLCEKFSIPYTEYSIAPY-DKIFGSHFKDASLTRKGNFCARLRMAFLYDY 121
Query: 119 ANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKK 178
+ + +V+GTSN+SE + GY T +GD A P+ L+KTEV+E+A + +P++I+ K
Sbjct: 122 SLKSDSLVIGTSNKSERMLGYGTLFGDLACAINPIGELFKTEVYELAYYLNIPKKILNKP 181
Query: 179 PTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAE---DLGIPIEKVEHVENLV 235
P+A L+ GQ+DE +LG Y ++D +L + L K E LG V+++ + +
Sbjct: 182 PSADLFVGQSDEKDLGYPYSVIDPLLKDIEALFQTKPIHLETLIQLGYAEILVKNIISRI 241
Query: 236 KGSEHKRRLP 245
+ + K LP
Sbjct: 242 QKNAFKLELP 251
>pir||T51756 NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) [imported] -
Streptomyces coelicolor (fragment)
>gi|4469317|emb|CAB38325.1| (Y17736) NAD(+) synthase
(glutamine-hydrolysing) [Streptomyces coelicolor A3(2)]
Length = 538
Score = 120 bits (297), Expect = 2e-26
Identities = 80/251 (31%), Positives = 121/251 (47%), Gaps = 17/251 (6%)
Query: 6 YEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQD 63
Y + + ++ + V++G+SGG+DSA V +A ALG E V G+ MP Y
Sbjct: 263 YSALVTGLRAYVAKNGFRSVLIGLSGGIDSALVASIACDALGAEHVYGVSMPSKYSSGHS 322
Query: 64 VEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLGNVMARTRMMILYAHANSMN 123
+DA + G+ YR ++I P+ D +EL + N+ +R R +L A +N
Sbjct: 323 RDDAAELARRTGLHYRTVSIEPMFDAYTG--ALELTGLAEENLQSRLRGTLLMALSNQEG 380
Query: 124 RMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIA-----------KRIGVPE 172
+VL N+SE GY T +GD Y P+ ++YKT V+ +A + VPE
Sbjct: 381 HLVLAPGNKSELAVGYSTLYGDSVGGYGPIKDVYKTSVFRLAEWRNRAARDRGRTPPVPE 440
Query: 173 RIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVE 232
+ K P+A L GQ D D L Y +LD +L VD ++I G E V
Sbjct: 441 NSITKPPSAELRPGQVDTDSLP-DYPVLDAVLALYVDRDRGADEIVA-AGYDRELVTRTL 498
Query: 233 NLVKGSEHKRR 243
+V +E+KRR
Sbjct: 499 RMVDTAEYKRR 509
>pir||C82617 NH3-dependent NAD synthetase XF1961 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9107063|gb|AAF84763.1|AE004015_6
(AE004015) NH3-dependent NAD synthetase [Xylella
fastidiosa]
Length = 545
Score = 120 bits (297), Expect = 2e-26
Identities = 81/255 (31%), Positives = 137/255 (52%), Gaps = 15/255 (5%)
Query: 1 MRTLDYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYE 60
+ L + + + D+ R+ + V VG+SGG+DSA V +A ALG ++V + +P
Sbjct: 265 INALVWRAVVRGVQDYCRKNGFSKVWVGLSGGIDSALVLAIAVDALGADQVTAVRLPSRY 324
Query: 61 NQDVED--AKLVVESLGIEYRVINIRPIVDTIVSEIGIEL----DKKSLGNVMARTRMMI 114
++ + A SLG+ + I P+ + +++ +G + N+ +R+R +I
Sbjct: 325 TAELSNDLAAEQCHSLGVRLETVAIEPVFEGLLAALGPLFAGMAPDATEENLQSRSRGVI 384
Query: 115 LYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK-------R 167
L A AN ++L T N+SE+ GY T +GD YAPL ++YK++V+E+A+
Sbjct: 385 LMALANKFGGLLLTTGNKSEYAVGYATIYGDMCGGYAPLKDIYKSQVFELAQWRNTVSDV 444
Query: 168 IGVPERIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEK 227
+ +P ++ + P+A L QTD+D L Y++LD IL VD + +E I G
Sbjct: 445 LAIPPGVIHRPPSAELRAQQTDQDSLP-PYEVLDGILSLYVDQEQSREDIIA-AGYAAGV 502
Query: 228 VEHVENLVKGSEHKR 242
V++V NLVK +E KR
Sbjct: 503 VDYVLNLVKINEWKR 517
>pir||H82488 NH(3)-dependent NAD(+) synthetase VCA0207 [imported] - Vibrio
cholerae (group O1 strain N16961)
>gi|9657599|gb|AAF96119.1| (AE004360) NH(3)-dependent
NAD(+) synthetase [Vibrio cholerae]
Length = 276
Score = 119 bits (296), Expect = 3e-26
Identities = 89/278 (32%), Positives = 144/278 (51%), Gaps = 44/278 (15%)
Query: 3 TLDYEKAIERIIDFIREKCSNG----VVVGISGGVDSAT---VTYLATKALGRE------ 49
++D + IER + FI+ K + +V+GISGGVDS T + LA + L ++
Sbjct: 14 SIDPQFEIERRVAFIKRKLTEARCKSLVLGISGGVDSTTCGRLAQLAVEELNQQHNTTEY 73
Query: 50 KVLGLIMPYYENQDVEDAKLVVESLGIEYRV-INIRPIVDTIVSEIGIELDKKSL----- 103
+ + + +PY E +D ++A+L + + + V +NI+ VD + + L L
Sbjct: 74 QFIAVRLPYGEQKDEDEAQLALSFIRPTHSVSVNIKAGVDGLHAASHHALANTGLIPSDP 133
Query: 104 -------GNVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINL 156
GNV AR RM+ Y A + +VLGT + +E +TG++TK+GDGA D APL L
Sbjct: 134 AKVDFIKGNVKARARMVAQYEIAGYVGGLVLGTDHSAENITGFYTKFGDGACDLAPLFGL 193
Query: 157 YKTEVWEIAKRIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKME 213
K +V +AK +G PE++V K PTA L E + DE L ++Y+ +D+ L
Sbjct: 194 NKRQVRLLAKTLGAPEQLVYKTPTADLEELAPQKADEAALNLTYEQIDDFL--------- 244
Query: 214 KEKIAEDLGIPIEKVEHVENLVKGSEHKRRLPIGPSFD 251
E +P E + + + ++HKR+ PI +D
Sbjct: 245 -----EGKAVPAEVSQRLVAIYHATQHKRQ-PIPTIYD 276
>pir||F83030 NH3-dependent NAD synthetase PA4920 [imported] - Pseudomonas
aeruginosa (strain PAO1)
>gi|9951198|gb|AAG08305.1|AE004905_3 (AE004905)
NH3-dependent NAD synthetase [Pseudomonas aeruginosa]
Length = 275
Score = 119 bits (295), Expect = 4e-26
Identities = 87/267 (32%), Positives = 136/267 (50%), Gaps = 37/267 (13%)
Query: 5 DYEKAIERIIDFIREKCSNG----VVVGISGGVDSATVTYLATKALGR---------EKV 51
D + I R I FI++ + +V+GISGGVDS T LA +A+ + +
Sbjct: 23 DVQAQIARRIAFIQQCLKDSGLKTLVLGISGGVDSLTAGLLAQRAVEQLREQTGDQAYRF 82
Query: 52 LGLIMPYYENQDVEDAKLVVESLGI-EYRVINIRPIVDTIVSEI----GIELDKKS--LG 104
+ + +PY QD DA+ + ++ E + +NI P V + ++ G+E K +G
Sbjct: 83 IAVRLPYQVQQDEADAQASLATIRADEEQTVNIGPSVKALAEQLEALEGLEPAKSDFVIG 142
Query: 105 NVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEI 164
N+ AR RM+ YA A + +V+GT + +E + G+FTK+GDGA D APL L K +V +
Sbjct: 143 NIKARIRMVAQYAIAGARGGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLAKHQVRAL 202
Query: 165 AKRIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDL 221
A+ +G PE +V+K PTA L + G DE G++Y +D L + + E+
Sbjct: 203 ARALGAPENLVEKIPTADLEDLRPGHPDEASHGVTYAEIDAFL--------HGQPLREEA 254
Query: 222 GIPIEKVEHVENLVKGSEHKRRLPIGP 248
I H ++HKR LP P
Sbjct: 255 ARVIVDTYH------KTQHKRELPKAP 275
>pir||A75617 NH3-dependent NAD+ synthase - Deinococcus radiodurans (strain R1)
>gi|6460473|gb|AAF12179.1|AE001862_5 (AE001862)
NH3-dependent NAD+ synthase [Deinococcus radiodurans]
Length = 287
Score = 117 bits (290), Expect = 1e-25
Identities = 82/259 (31%), Positives = 125/259 (47%), Gaps = 29/259 (11%)
Query: 5 DYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREK-------VLGLIMP 57
+ E+ + + D+++ + G V+GISGG DS L A+ R + L + +P
Sbjct: 30 EVERRVAFLCDYLQSTPTKGFVLGISGGQDSTLAGRLCQLAVERRRSQGHGATFLAVRLP 89
Query: 58 YYENQDVEDAKLVVESLGIEYRV-INIRPIVDTIVSE----IGIELDKKSLGNVMARTRM 112
Y D DA+ ++ + + V +NI+ D V+ +G E+ GNV AR RM
Sbjct: 90 YGVQADEADAQQALDFIQADREVTVNIKEAADASVAAAQAALGSEVRDFVRGNVKARERM 149
Query: 113 MILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPE 172
+ YA A N +V+GT + +E LTG++TK+GDG D PL L K + ++ +G PE
Sbjct: 150 VAQYALAGQENLLVVGTDHAAEALTGFYTKYGDGGVDLTPLSGLTKRQGAQLLAHLGAPE 209
Query: 173 RIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVE 229
+K PTA L + G DE LG++Y +D L E + E
Sbjct: 210 GTWRKVPTADLEDDRPGLPDEVALGVTYAQIDAYL--------------EGREVSDEAAA 255
Query: 230 HVENLVKGSEHKRRLPIGP 248
+E L S HKR LP+ P
Sbjct: 256 RLERLFLNSRHKRALPVTP 274
>pir||C81850 NAD+ synthase (glutamine-hydrolyzing) (EC 6.3.5.1) NMA1576
[similarity] - Neisseria meningitidis (group A strain
Z2491) >gi|7380217|emb|CAB84803.1| (AL162756)
NH(3)-dependent NAD synthetase [Neisseria meningitidis
Z2491]
Length = 273
Score = 117 bits (290), Expect = 1e-25
Identities = 86/255 (33%), Positives = 136/255 (52%), Gaps = 24/255 (9%)
Query: 4 LDYEKAIERIIDFIREKCSN----GVVVGISGGVDSATVTYLATKALGREKVLGLIMPYY 59
+D + I I+ ++ E + G VVG+SGG+DSA V+ LA + GR +L L MP
Sbjct: 11 MDTQAVITHIVRWLDEYAARANAKGFVVGVSGGIDSAVVSALAART-GRTTLL-LDMPIR 68
Query: 60 ENQD-VEDAKLVVESLGIEYRVINIRPI--VDTI--------VSEIGIELDKKSLGNVMA 108
++ D +E A+L + +L +Y ++ + + DT V + + SL N +
Sbjct: 69 QHPDQLERARLHIRNLQRQYANVSAQTVDLTDTFQTFEQTVGVHQTAFDNQPLSLANARS 128
Query: 109 RTRMMILYAHANSMNRMVLGTSNRSE-FLTGYFTKWGDGASDYAPLINLYKTEVWEIAKR 167
R RM+ LY + +V GT N+ E F G+FTK+GDG D +P+ +L KT+V+ +A+
Sbjct: 129 RLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGFFTKYGDGGVDISPIADLTKTQVYRLAEA 188
Query: 168 IGVPERIVKKKPTAGLWEGQ-TDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIE 226
+GV E I K PT GLW+ + TDE+++G SY E+ W + K + E G E
Sbjct: 189 LGVDEAIQKAPPTDGLWDTERTDEEQMGASY---PELEWAMGVYGTRKPEDFE--GRQRE 243
Query: 227 KVEHVENLVKGSEHK 241
+E L + +HK
Sbjct: 244 VLEIYTRLHRAMQHK 258
>sp|Q03638|NADE_RHOCA GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE (NAD(+) SYNTHASE
[GLUTAMINE-HYDROLYSING]) >gi|97438|pir||S15555 NAD+
synthase (EC 6.3.1.5) adgA [validated] - Rhodobacter
capsulatus >gi|45986|emb|CAA42042.1| (X59399) NAD
synthetase [Rhodobacter capsulatus]
Length = 552
Score = 114 bits (282), Expect = 1e-24
Identities = 87/259 (33%), Positives = 131/259 (49%), Gaps = 24/259 (9%)
Query: 5 DYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMP--YYENQ 62
DY + + D++R+ + VV+G+SGG+DSA V +A ALG V +++P Y
Sbjct: 267 DYRAMVLGLQDYLRKSGFSRVVLGLSGGIDSALVAVIAADALGAGNVHCVMLPSRYTSQG 326
Query: 63 DVEDAKLVVESLGIEYRVINI---RPIVDTIVSEI--GIELDKKSLGNVMARTRMMILYA 117
++DA + LG + I R V+ ++ + G D N+ +R R +IL A
Sbjct: 327 SLDDAADLARRLGARLDTVEIEGPRAAVEGALAHVLAGTAPDVTE-ENIQSRLRGVILMA 385
Query: 118 HANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIG-------- 169
+N M+L T N+SE GY T +GD A Y PL +LYKT V+E +
Sbjct: 386 ISNKFGAMLLTTGNKSEVAVGYCTIYGDMAGGYNPLKDLYKTRVFETCRWRNATHRPWMQ 445
Query: 170 ------VPERIVKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGI 223
+P I+ K P+A L E QTD+D L Y++LD IL RLV+ ++I G
Sbjct: 446 APAGEIIPVAIIDKPPSAELRENQTDQDSLP-PYEVLDAILERLVEGDQSVDQIVA-AGF 503
Query: 224 PIEKVEHVENLVKGSEHKR 242
V+ +E+L+ SE KR
Sbjct: 504 DRATVKRIEHLLYISEWKR 522
>pir||A81090 NH(3)-dependent NAD+ synthetase NadE, probable NMB1364 [imported] -
Neisseria meningitidis (group B strain MD58)
>gi|7226610|gb|AAF41738.1| (AE002485) NH(3)-dependent
NAD+ synthetase NadE, putative [Neisseria meningitidis
MC58]
Length = 273
Score = 112 bits (278), Expect = 4e-24
Identities = 80/233 (34%), Positives = 125/233 (53%), Gaps = 20/233 (8%)
Query: 22 SNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQ-DVEDAKLVVESLGIEY-- 78
+ G VVG+SGG+DSA V+ LA + GR +L L MP ++ +E A+L + +L +Y
Sbjct: 33 AKGFVVGVSGGIDSAVVSALAART-GRPTLL-LDMPIRQHPGQLERARLHIRNLQRQYAN 90
Query: 79 ---RVINIRPIVDTIVSEIG-----IELDKKSLGNVMARTRMMILYAHANSMNRMVLGTS 130
+ +++ T +G + SL N +R RM+ LY + +V GT
Sbjct: 91 VSAQTVDLTDTFQTFEQTVGAHQTAFDSQPLSLANARSRLRMLTLYYYGQIHGLLVTGTG 150
Query: 131 NRSE-FLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQ-T 188
N+ E F G+FTK+GDG D +P+ +L KT+V+ +A+ +GV E I K PT GLW+ + T
Sbjct: 151 NKIEDFGVGFFTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERT 210
Query: 189 DEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHK 241
DE+++G SY E+ W + K + E G E +E L + +HK
Sbjct: 211 DEEQMGASY---PELEWAMGVYGTRKPEDFE--GRQREVLEIYTRLHRAMQHK 258
>sp|P18843|NADE_ECOLI NH(3)-DEPENDENT NAD(+) SYNTHETASE (NITROGEN-REGULATORY PROTEIN)
>gi|7443246|pir||D64933 NAD+ synthase (EC 6.3.1.5) nadE
[validated] - Escherichia coli (strain K-12)
>gi|1742846|dbj|BAA15529.1| (D90817) NH(3)-dependent
NAD(+) synthetase (EC 6.3.5.1) (Nitrogen-regulatory
protein). [Escherichia coli] >gi|1742853|dbj|BAA15535.1|
(D90818) NH(3)-dependent NAD(+) synthetase (EC 6.3.5.1)
(Nitrogen-regulatory protein). [Escherichia coli]
>gi|1788036|gb|AAC74810.1| (AE000269) NAD synthetase,
prefers NH3 over glutamine [Escherichia coli K12]
Length = 275
Score = 109 bits (270), Expect = 3e-23
Identities = 85/272 (31%), Positives = 132/272 (48%), Gaps = 40/272 (14%)
Query: 4 LDYEKAIERIIDFIREKCSN-----GVVVGISGGVDSATVTYLATKAL-------GREKV 51
++ E+ I R +DF++ +V+GISGG DS L A+ G E +
Sbjct: 17 INAEEEIRRSVDFLKSYLQTYPFIKSLVLGISGGQDSTLAGKLCQMAINELRLETGNESL 76
Query: 52 --LGLIMPY---YENQDVEDAKLVVESLGIEYRVINIRPIV---DTIVSEIGIELDKKSL 103
+ + +PY + QD +DA ++ + +NI+ V + + E GIEL
Sbjct: 77 QFIAVRLPYGVQADEQDCQDAIAFIQPDRV--LTVNIKGAVLASEQALREAGIELSDFVR 134
Query: 104 GNVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWE 163
GN AR RM Y+ A + +V+GT + +E +TG+FTK+GDG +D PL L K + +
Sbjct: 135 GNEKARERMKAQYSIAGMTSGVVVGTDHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQ 194
Query: 164 IAKRIGVPERIVKKKPTAGLWEGQ---TDEDELGISYKLLDEILWRLVDLKMEKEKIAED 220
+ + PE + KK PTA L + + DE LG++Y +D+ L E
Sbjct: 195 LLAALACPEHLYKKAPTADLEDDRPSLPDEVALGVTYDNIDDYL--------------EG 240
Query: 221 LGIPIEKVEHVENLVKGSEHKRRLPIGPSFDD 252
+P + +EN +EHKRR PI FDD
Sbjct: 241 KNVPQQVARTIENWYLKTEHKRRPPI-TVFDD 271
>pir||A26928 nitrogen-regulatory protein - Escherichia coli
>gi|146974|gb|AAA79852.1| (M15328) NH3-dependent NAD
synthetase [Escherichia coli]
Length = 274
Score = 104 bits (257), Expect = 1e-21
Identities = 80/246 (32%), Positives = 121/246 (48%), Gaps = 35/246 (14%)
Query: 25 VVVGISGGVDSATVTYLATKAL-------GREKV--LGLIMPY---YENQDVEDAKLVVE 72
+V+GISGG DS L A+ G E + + + +PY + QD +DA ++
Sbjct: 42 LVLGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ 101
Query: 73 SLGIEYRVINIRPIV---DTIVSEIGIELDKKSLGNVMARTRMMILYAHANSMNRMVLGT 129
+ +NI+ V + + E GIEL GN AR RM Y+ A + +V+GT
Sbjct: 102 PDRV--LTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT 159
Query: 130 SNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLWEGQ-- 187
+ +E +TG+FTK+GDG +D PL L K + ++ + PE + KK PTA L + +
Sbjct: 160 DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDRPS 219
Query: 188 -TDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEKVEHVENLVKGSEHKRRLPI 246
DE LG++Y +D+ L E +P + +EN +EHKRR PI
Sbjct: 220 LPDEVALGVTYDNIDDYL--------------EGKNVPQQVARTIENWYLKTEHKRRPPI 265
Query: 247 GPSFDD 252
FDD
Sbjct: 266 -TVFDD 270
>dbj|BAB06004.1| (AP001515) NH3-dependent NAD synthetase [Bacillus halodurans]
Length = 272
Score = 102 bits (253), Expect = 3e-21
Identities = 76/259 (29%), Positives = 125/259 (47%), Gaps = 31/259 (11%)
Query: 5 DYEKAIERIIDFIREKCSNGVVVGISGGVDSATVTYLATKA---LGREK------VLGLI 55
+ K + + ++++ + G V+G+SGG DS LA A L E+ + +
Sbjct: 20 EIRKRVTFLKNYLKHSGAKGYVLGLSGGQDSTLAGKLAQMAIDELNEEEQDTSYVFIAVR 79
Query: 56 MPYYENQDVEDAKLVVESLGIEYRV-INIRPIVDTIVSEI----GIELDKKSLGNVMART 110
+PY +D DA+ + + + +NI+ VD G L + GN AR
Sbjct: 80 LPYGVQKDEADAQDAIAFIKPSRSITVNIKDAVDASTKSFEQATGEVLSDFNKGNTKARE 139
Query: 111 RMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGV 170
RM Y +V+GT + +E +TG+FTK GDGA D APL L K + + K +G
Sbjct: 140 RMKAQYDVGAHYGCLVIGTDHAAEAITGFFTKHGDGACDVAPLFGLTKRQGKSLLKELGA 199
Query: 171 PERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEK 227
P + K PTA L + G DE+ LG++Y+ LD+ L E +++ + +
Sbjct: 200 PTHLYTKAPTADLEDDRPGLPDEEALGLTYEQLDDYL--------EGKQVHDAIR----- 246
Query: 228 VEHVENLVKGSEHKRRLPI 246
+ +E+ +EHKR+LP+
Sbjct: 247 -KKIESRYLATEHKRQLPV 264
>sp|P57271|NADE_BUCAI NH(3)-DEPENDENT NAD(+) SYNTHETASE >gi|10038856|dbj|BAB12891.1|
(AP001118) nh(3)-dependent NAD(+) synthetase [Buchnera
sp. APS]
Length = 268
Score = 100 bits (246), Expect = 2e-20
Identities = 78/263 (29%), Positives = 127/263 (47%), Gaps = 39/263 (14%)
Query: 7 EKAIERIIDFIREKCSNGV-----VVGISGGVDS---ATVTYLATKALGREK------VL 52
E I+ +DF+++ N V +VG+SGG DS A + + + L +EK +
Sbjct: 20 EIEIKNCVDFLKKYLVNHVHIKSLIVGVSGGQDSTLTAKLCQMTAETLRKEKNDITYQFI 79
Query: 53 GLIMPY---YENQDVEDAKLVVESLGIEYRVINIRPIV---DTIVSEIGIELDKKSLGNV 106
L +PY Y+ +D +DA ++ I +NI+ V + + + G+ + GN
Sbjct: 80 ALRLPYGIQYDEKDCQDAIRFIQPDQIFN--VNIKKAVLSSEKSLKKSGVIISDYVRGNE 137
Query: 107 MARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAK 166
AR RM + Y+ A +V+GT + +E +TG+FTK+GD +D P+ L K ++ + K
Sbjct: 138 KARERMKVQYSIAAMKQGLVVGTGHAAENITGFFTKYGDSGTDINPIAKLNKRQIRLLLK 197
Query: 167 RIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGI 223
+ P+ + KKP A L + Q DE LG++Y +D L E I
Sbjct: 198 NLNCPKHLYLKKPMADLEDEHPQQDDESVLGVTYDAIDSYL--------------EQKKI 243
Query: 224 PIEKVEHVENLVKGSEHKRRLPI 246
I + +E L + HKR LPI
Sbjct: 244 DIRSQKIIEALYLNTLHKRNLPI 266
>pir||E82888 NH(3)-dependent NAD(+) synthetase UU460 [imported] - Ureaplasma
urealyticum >gi|6899453|gb|AAF30872.1|AE002142_6
(AE002142) NH(3)-dependent NAD(+) synthetase [Ureaplasma
urealyticum]
Length = 255
Score = 95.2 bits (233), Expect = 7e-19
Identities = 68/209 (32%), Positives = 109/209 (51%), Gaps = 14/209 (6%)
Query: 14 IDFIREKCSNGVVVGISGGVDSATVTYLATKALGREKVLGLIMPYYENQDVEDAKLVVES 73
I F+ + +N V++GISGG+DS T+T L +E L + Y D+ ++ L E
Sbjct: 17 IKFLNDAQTNNVILGISGGIDS-TLTLAILNDLKKEYDLNI---YAYFLDIHNSSLDYEC 72
Query: 74 LGI------EYRVINIRPIVDTIVSEIGIEL-DKKSLGNVMARTRMMILYAHANSMNRMV 126
+ VIN+ I D+ I + DK L N+ + R LYA AN+ +V
Sbjct: 73 INELKNIYPNIEVINLVDIYDSYCKMINKKTNDKYVLYNLKPKIRTNYLYAMANAYKGVV 132
Query: 127 LGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIGVPERIVKKKPTAGLW-- 184
+ N E++ G+FTK+GD A+DY LI L K ++E+ +P +I+ + PT
Sbjct: 133 VSNLNYDEYILGFFTKYGDSAADYYMLIGLLKKHIYELGAYYHLPNKILNRAPTPANEDD 192
Query: 185 EGQTDEDELGISYKLLDE-ILWRLVDLKM 212
E +TDE G +Y LD+ +L+R ++ K+
Sbjct: 193 EHKTDESFFGFTYNDLDQFLLYRKINPKI 221
>gb|AAG27700.1|AF299296_3 (AF299296) NH(3)-dependent NAD(+)synthetase [Pseudomonas putida]
Length = 275
Score = 90.1 bits (220), Expect = 2e-17
Identities = 76/231 (32%), Positives = 110/231 (46%), Gaps = 39/231 (16%)
Query: 4 LDYEKAIERIIDFIREKCSNG----VVVGISGGVDSATVTYLATKALG--REKV------ 51
L+ EKA R + FI++ N +V+GISGGVDS T LA +A+ RE+
Sbjct: 24 LEAEKA--RRVAFIQQCLKNAGLKTLVLGISGGVDSLTGALLAQRAINELREQTGDVHYR 81
Query: 52 -LGLIMPYYENQDVEDAKLVVESLGIEYRVINIRPIVDTIVSEIGIELDKKSLG------ 104
+ + +PY D DA V +R R + G + G
Sbjct: 82 FIAVRLPYQVQHDEHDAHGV-------HRFHPARRMTYGEHRAGGAGAGGRGRGAQGWQA 134
Query: 105 --------NVMARTRMMILYAHANSMNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINL 156
N AR RM+ Y A + +V+ T + +E + G+FTK+GDGA D APL L
Sbjct: 135 GAGGFRGGNTKARMRMVAQYTIAGARGGLVIDTDHAAEAVMGFFTKFGDGACDLAPLSGL 194
Query: 157 YKTEVWEIAKRIGVPERIVKKKPTAGLWE---GQTDEDELGISYKLLDEIL 204
K +V +IA+ G PE +V+K PTA L + G+ DE G++Y +D L
Sbjct: 195 VKHQVRDIARSFGAPESLVEKVPTADLEDLAPGKPDEASHGVTYAQIDAFL 245
>pir||A70165 NH(3)-dependent NAD+ synthetase homolog - Lyme disease spirochete
>gi|2688436|gb|AAC66885.1| (AE001154) NH(3)-dependent
NAD+ synthetase [Borrelia burgdorferi]
Length = 222
Score = 75.3 bits (182), Expect = 7e-13
Identities = 60/191 (31%), Positives = 101/191 (52%), Gaps = 16/191 (8%)
Query: 64 VEDAKLVVESLGIEYRVINIRPI--VDTIVSEIGIELDKKSLGNVMARTRMMILYAHANS 121
+ DAK + LG + + I+ + V + E ++ + N+ AR R ++L +++NS
Sbjct: 11 ISDAKELSRKLGFKLIEMPIKDLFQVSSRFFEGYFDIKGVTGENLQARLRGLLLMSYSNS 70
Query: 122 MNRMVLGTSNRSEFLTGYFTKWGDGASDYAPLINLYKTEVWEIAKRIG-------VPERI 174
N ++L T N+SE GY T +GD A + +L+KTEV+++ I +P I
Sbjct: 71 QNSLLLNTGNKSEIAVGYCTLYGDTCGGLALIGDLFKTEVYDLVNYINAKFDQCIIPVNI 130
Query: 175 VKKKPTAGLWEGQTDEDELGISYKLLDEILWRLVDLKMEKEKIAEDLGIPIEK--VEHVE 232
+ K+P+A L Q D D L Y++LD IL R + + E + + + + EK V+ V
Sbjct: 131 ILKEPSAELRFDQKDSDYLP-KYEVLDVILNRYL---IGNESV-DSIYLNFEKGIVDKVL 185
Query: 233 NLVKGSEHKRR 243
NL +E+KRR
Sbjct: 186 NLYFKNEYKRR 196
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.139 0.396
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94865248
Number of Sequences: 2977
Number of extensions: 3939638
Number of successful extensions: 11276
Number of sequences better than 1.0e-10: 40
Number of HSP's better than 0.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 11163
Number of HSP's gapped (non-prelim): 42
length of query: 257
length of database: 189,106,746
effective HSP length: 56
effective length of query: 201
effective length of database: 155,591,474
effective search space: 31273886274
effective search space used: 31273886274
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 164 (68.3 bits)