BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB2248 (PAB2248) DE:BIOTIN SYNTHASE, PUTATIVE. (163 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||H75203 probable biotin synthase PAB2248 - Pyrococcus abyssi... 313 9e-85 sp|O57898|Y159_PYRHO HYPOTHETICAL PROTEIN PH0159 >gi|7518498|pir... 242 2e-63 pir||H72334 bioY protein - Thermotoga maritima (strain MSB8) >gi... 128 4e-29 sp|O83256|Y228_TREPA HYPOTHETICAL PROTEIN TP0228 >gi|7520944|pir... 97 1e-19 sp|P22819|BIOY_BACSH BIOY PROTEIN >gi|98186|pir||JQ0508 bioY pro... 94 1e-18 dbj|BAB07077.1| (AP001518) biotin synthesis BioY proein [Bacillu... 90 1e-17 gb|AAG20426.1| (AE005115) Vng2316c [Halobacterium sp. NRC-1] 87 2e-16 sp|Q9ZDK4|Y324_RICPR HYPOTHETICAL PROTEIN RP324 >gi|7467713|pir|... 87 2e-16 gi|11498769 conserved hypothetical protein [Archaeoglobus fulgid... 86 2e-16 pir||H70012 biotin metabolism homolog yuiG - Bacillus subtilis >... 83 1e-15 pir||C75270 probable biotin synthase - Deinococcus radiodurans (... 83 1e-15 emb|CAB71805.1| (AL138661) putative biotin synthase [Streptomyce... 83 2e-15 pir||B69832 biotin biosynthesis homolog yhfU - Bacillus subtilis... 79 3e-14 sp|O26990|Y905_METTH HYPOTHETICAL PROTEIN MTH905 >gi|7482274|pir... 71 7e-12 pir||D71524 hypothetical protein CT359 - Chlamydia trachomatis (... 71 1e-11 >pir||H75203 probable biotin synthase PAB2248 - Pyrococcus abyssi (strain Orsay) >gi|5457588|emb|CAB49079.1| (AJ248283) BIOTIN SYNTHASE, PUTATIVE. [Pyrococcus abyssi] Length = 163 Score = 313 bits (793), Expect = 9e-85 Identities = 163/163 (100%), Positives = 163/163 (100%) Query: 1 MKAKEVSLVALFTALTAVGAQISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVL 60 MKAKEVSLVALFTALTAVGAQISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVL Sbjct: 1 MKAKEVSLVALFTALTAVGAQISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVL 60 Query: 61 GMLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKVKYLTSLASLGVIYSLGW 120 GMLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKVKYLTSLASLGVIYSLGW Sbjct: 61 GMLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKVKYLTSLASLGVIYSLGW 120 Query: 121 LRLSSFIGAEKALKVGVLPFILPDIVKILVAIAIVNRLNIRRP 163 LRLSSFIGAEKALKVGVLPFILPDIVKILVAIAIVNRLNIRRP Sbjct: 121 LRLSSFIGAEKALKVGVLPFILPDIVKILVAIAIVNRLNIRRP 163 >sp|O57898|Y159_PYRHO HYPOTHETICAL PROTEIN PH0159 >gi|7518498|pir||E71237 hypothetical protein PH0159 - Pyrococcus horikoshii >gi|3256545|dbj|BAA29228.1| (AP000001) 162aa long hypothetical protein [Pyrococcus horikoshii] Length = 162 Score = 242 bits (611), Expect = 2e-63 Identities = 119/158 (75%), Positives = 137/158 (86%) Query: 1 MKAKEVSLVALFTALTAVGAQISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVL 60 MKAKEVSLVALF ALT+VGAQIS+SIGPVPLTLQV FV+LAGLVLGP+LGFLS+L+YD+L Sbjct: 1 MKAKEVSLVALFVALTSVGAQISVSIGPVPLTLQVFFVILAGLVLGPKLGFLSILIYDIL 60 Query: 61 GMLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKVKYLTSLASLGVIYSLGW 120 G LG PVFAGFSGGIAHILGPTGGYI+AFPIA+ IAGLG+GKV+YLTSL L VIY +GW Sbjct: 61 GALGIPVFAGFSGGIAHILGPTGGYIIAFPIATFIAGLGRGKVRYLTSLMGLLVIYLIGW 120 Query: 121 LRLSSFIGAEKALKVGVLPFILPDIVKILVAIAIVNRL 158 + L+ F+G KA +GVLPFI+PDIVK A+ I RL Sbjct: 121 MWLARFVGFNKAFILGVLPFIVPDIVKAATAVIIAERL 158 >pir||H72334 bioY protein - Thermotoga maritima (strain MSB8) >gi|4981328|gb|AAD35881.1|AE001747_24 (AE001747) bioY protein [Thermotoga maritima] Length = 169 Score = 128 bits (318), Expect = 4e-29 Identities = 71/161 (44%), Positives = 102/161 (63%), Gaps = 6/161 (3%) Query: 4 KEVSLVALFTALTAVGAQISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVLGML 63 +++ +FTAL VGA ISI +GPVP TLQV FV L+ VLG + G L++ Y +LG + Sbjct: 2 RQLIKAGIFTALIVVGAWISIPLGPVPFTLQVFFVFLSAYVLGKKYGTLAVATYVLLGAM 61 Query: 64 GFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKVK----YLTS-LASLGVIYSL 118 G PVFA F GG ++GPTGGY+ F + + + GL K + YL S +A LG+IY+L Sbjct: 62 GLPVFANFKGGAQVLVGPTGGYLFGFILGAFVIGLLAEKKESFAWYLASGVAGLGIIYAL 121 Query: 119 GWLRLSSFI-GAEKALKVGVLPFILPDIVKILVAIAIVNRL 158 G L+ ++ KA+ VG +PF+ D++K++VA I RL Sbjct: 122 GVFVLNFYVHDIRKAISVGFVPFVWFDLIKLVVAALIALRL 162 >sp|O83256|Y228_TREPA HYPOTHETICAL PROTEIN TP0228 >gi|7520944|pir||B71351 probable biotin synthase - syphilis spirochete >gi|3322497|gb|AAC65216.1| (AE001204) biotin synthase, putative [Treponema pallidum] Length = 271 Score = 97.1 bits (238), Expect = 1e-19 Identities = 64/178 (35%), Positives = 95/178 (52%), Gaps = 21/178 (11%) Query: 2 KAKEVSLVALFTALTAVGAQISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVLG 61 ++K ++ VALF AL + A +SI + PVPL LQ VL GL+LGPR G L++L + G Sbjct: 82 RSKSLAFVALFAALISSSALVSIPLKPVPLVLQNAAAVLTGLLLGPRDGALAVLSFLGAG 141 Query: 62 MLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGL-------------GKGKVKYL-- 106 +LG PVF+G GG + PTGG+++ + +A+ +AG G G+ L Sbjct: 142 LLGLPVFSGGRGGYTALFAPTGGFLLGYILAATLAGAIAQHHRLSCTPPRGGGRALLLWI 201 Query: 107 ----TSLASLGVIYSLGWLRLSSFIGAEKA-LKVGV-LPFILPDIVKILVAIAIVNRL 158 +L IYS+G L +G L +GV LPF L D +KI + + + + L Sbjct: 202 RLTVATLVGFLSIYSIGLPVLGYVLGLRTGELMLGVFLPFFLADTLKIALVVLLAHHL 259 >sp|P22819|BIOY_BACSH BIOY PROTEIN >gi|98186|pir||JQ0508 bioY protein - Bacillus sphaericus >gi|142590|gb|AAB02326.1| (M29292) biotin synthase [Bacillus sphaericus] Length = 196 Score = 93.6 bits (229), Expect = 1e-18 Identities = 59/168 (35%), Positives = 91/168 (54%), Gaps = 20/168 (11%) Query: 8 LVALFTALTAVGAQISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVLGMLGFPV 67 ++A+F ALTAVGA I I + VP TLQ++FV LAG +LG R GF S L+Y +G++G PV Sbjct: 12 MIAMFAALTAVGAFIKIPLPLVPFTLQIVFVFLAGCLLGGRNGFQSQLVYIGIGLVGLPV 71 Query: 68 FAGFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKVK-------YLTSLASLGVIYSLG- 119 F GGI ++L PT GY++ F +A+L+ G +V+ + ++ L +IY++ Sbjct: 72 FTQ-GGGITYVLQPTFGYLIGFALAALVIGYMIDRVESPTKKHFIVANIIGLIIIYAVAV 130 Query: 120 ---------WLRLSSFIGAEKALKVGVLPFILPDIVKILVAIAIVNRL 158 WL + S VG + I+ D + + + RL Sbjct: 131 PYLYVALNVWLNMKS--SWSHVFLVGFVNSIVADFCLAIASALLAERL 176 >dbj|BAB07077.1| (AP001518) biotin synthesis BioY proein [Bacillus halodurans] Length = 197 Score = 90.1 bits (220), Expect = 1e-17 Identities = 42/95 (44%), Positives = 67/95 (70%), Gaps = 2/95 (2%) Query: 5 EVSLVALFTALTAVGAQIS--ISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVLGM 62 ++ LV +F AL +GA ++ + IG VP+TLQ LF +LAG +LG RLG +M LY ++G+ Sbjct: 10 DLVLVPMFAALMGIGANVTSFLVIGGVPITLQTLFSILAGALLGSRLGAFAMSLYLLVGL 69 Query: 63 LGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAG 97 +GFPVFA F GG++ + PT G++++F + + + G Sbjct: 70 VGFPVFAQFKGGLSTFVSPTFGFLISFILVAYVTG 104 >gb|AAG20426.1| (AE005115) Vng2316c [Halobacterium sp. NRC-1] Length = 202 Score = 86.6 bits (211), Expect = 2e-16 Identities = 44/97 (45%), Positives = 62/97 (63%), Gaps = 2/97 (2%) Query: 4 KEVSLVALFTALTAVGAQISIS--IGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVLG 61 + ++ A+F ALT A +S + P P+TLQVL V LAGL LGP G SM+LY + G Sbjct: 17 ENIARAAVFAALTGAMAYVSFQNPVSPAPVTLQVLGVFLAGLYLGPVWGGASMVLYLIAG 76 Query: 62 MLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGL 98 +G PVFAG G+ + PTGGY++++P A+ + GL Sbjct: 77 GVGAPVFAGGGSGLGTFVAPTGGYLLSYPFAAALTGL 113 >sp|Q9ZDK4|Y324_RICPR HYPOTHETICAL PROTEIN RP324 >gi|7467713|pir||F71688 hypothetical protein RP324 - Rickettsia prowazekii >gi|3860884|emb|CAA14784.1| (AJ235271) unknown [Rickettsia prowazekii] Length = 194 Score = 86.6 bits (211), Expect = 2e-16 Identities = 54/164 (32%), Positives = 93/164 (55%), Gaps = 12/164 (7%) Query: 6 VSLVALFTALTAVGAQISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVLGMLGF 65 + ++A+F + +QI+I I PV +T+ + + + PRL FL++L + +G++G Sbjct: 35 MGVIAIFAS-----SQITIPIKPVAITMHSVILCIIAFTYSPRLSFLTILTFIFIGVMGL 89 Query: 66 PVFAGFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKV--KYL--TSLASLG--VIYSLG 119 PVF FS GI + LG GGY + F I + + KGK+ YL T + +G + Y G Sbjct: 90 PVFCQFSSGINYFLGSAGGYYLGFLIGMPVMSVLKGKLAENYLNVTIICIIGHAIFYLCG 149 Query: 120 WLRLSSFIGAEKALKVGVLPFILPDIVKILVAIAIVNRL-NIRR 162 + L+S IG ++A+ G + FI +VKIL+ + + + N++R Sbjct: 150 IIWLASMIGLKQAIYSGFIIFIPSGLVKILIFSCLFSYIKNLKR 193 >gi|11498769 conserved hypothetical protein [Archaeoglobus fulgidus] >gi|7483095|pir||H69395 conserved hypothetical protein AF1169 - Archaeoglobus fulgidus >gi|2649413|gb|AAB90071.1| (AE001023) conserved hypothetical protein [Archaeoglobus fulgidus] Length = 206 Score = 86.2 bits (210), Expect = 2e-16 Identities = 56/170 (32%), Positives = 88/170 (50%), Gaps = 20/170 (11%) Query: 4 KEVSLVALFTALTAVGAQISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVLGML 63 K+ +L + +TAV AQ+S IGPVP T+Q V+L+G +LGP G LSM +Y L Sbjct: 6 KKTALAVVTALITAVSAQLSFKIGPVPYTMQNFGVMLSGFLLGPYYGLLSMAIYLALIAF 65 Query: 64 GFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKV-----------KYLTSLASL 112 P+ AG GG+ ++GPT G++ F I+S +AG+ + + +L++L + Sbjct: 66 SLPIAAG-GGGLGVLIGPTAGFLYGFLISSFLAGVFRRAIWREGSRREVLMLWLSTLVAS 124 Query: 113 GVIYSLGWLRLSSF--------IGAEKALKVGVLPFILPDIVKILVAIAI 154 IY LG+ +F I +E A++ L F P +V + I Sbjct: 125 MPIYLLGFFVFYNFAMIDEKLLIRSESAVRAFNLSFSSPTMVIFAATVLI 174 >pir||H70012 biotin metabolism homolog yuiG - Bacillus subtilis >gi|2635700|emb|CAB15193.1| (Z99120) similar to biotin metabolism [Bacillus subtilis] Length = 200 Score = 83.5 bits (203), Expect = 1e-15 Identities = 44/131 (33%), Positives = 80/131 (60%), Gaps = 12/131 (9%) Query: 1 MKAKEVSLVALFTALTAVGAQIS-----ISIGPVPLTLQVLFVVLAGLVLGPRLGFLSML 55 ++A +++L+ +F AL AVGA I+ + + +PL++Q F +LA L+LG + ++M+ Sbjct: 6 LRAGDMALIGMFAALMAVGANITSVAPFLQVAGIPLSMQPFFCLLAALLLGSKRAAIAMI 65 Query: 56 LYDVLGMLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAG------LGKGKVKYL-TS 108 +Y ++G+ G PVFA FS G A G +GG+I+++ A+ AG + K+++L S Sbjct: 66 VYALVGLAGAPVFAQFSAGFAPFAGKSGGFIISYIPAAFAAGWFLERNIQPSKIRFLIAS 125 Query: 109 LASLGVIYSLG 119 L ++Y +G Sbjct: 126 LIGTAIMYLIG 136 >pir||C75270 probable biotin synthase - Deinococcus radiodurans (strain R1) >gi|6460290|gb|AAF12013.1|AE002076_5 (AE002076) biotin synthase, putative [Deinococcus radiodurans] Length = 229 Score = 83.5 bits (203), Expect = 1e-15 Identities = 57/168 (33%), Positives = 95/168 (55%), Gaps = 16/168 (9%) Query: 1 MKAKEVSLVALFTALTAVGA---QISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLY 57 M K++ +ALF A+ A A I+++ G VP++ Q L +LAG VLG R G LSM+L+ Sbjct: 43 MTTKDIVYIALFAAIMAALAVVPPITLATG-VPISAQALGPMLAGAVLGARRGALSMVLF 101 Query: 58 DVLGMLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGL----GKGKVKYLTSLA--- 110 VL G P+ G GGI+ GPT G+++++PIA+ + GL ++ + + A Sbjct: 102 LVLVAAGLPLLVGGRGGISAFAGPTAGFLISWPIAAFVIGLLVERNWARLNFALAFAIIF 161 Query: 111 --SLGVIYSLG--WLRLSSFIGAEKALKVGVLPFILPDIVKILVAIAI 154 S+ ++Y +G WL S+ + KA + PF+ D++K ++A + Sbjct: 162 VSSVVIMYGIGNAWLSYSAPMSYLKA-TIAAGPFLPGDLLKSVLAALV 208 >emb|CAB71805.1| (AL138661) putative biotin synthase [Streptomyces coelicolor A3(2)] Length = 247 Score = 82.7 bits (201), Expect = 2e-15 Identities = 55/162 (33%), Positives = 89/162 (53%), Gaps = 8/162 (4%) Query: 2 KAKEVSLVALFTALTAVGAQISISI--GPVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDV 59 + ++V+LVA LT + AQI++ + PVP+T Q +L G LG R G LS+ LY + Sbjct: 76 RVRDVALVAGGAVLTGIAAQIAVPVPGSPVPVTGQTFAALLVGTSLGARRGLLSLALYAL 135 Query: 60 LGMLGFPVFA-GFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKVKYLTS---LASLGVI 115 LGM G P FA G SG A LG G ++A + +A G + T+ L +I Sbjct: 136 LGMAGVPWFAEGGSGTAAPSLGYVFGMLLAATVVGALARRGADRSVLRTAGTMLLGEAII 195 Query: 116 YSLG--WLRLSSFIGAEKALKVGVLPFILPDIVKILVAIAIV 155 Y++G +L L++ + A+ G+ PF++ D +K +A+ ++ Sbjct: 196 YAVGVPYLALAADMSLTAAIAAGLTPFLIGDALKAALAMGVL 237 >pir||B69832 biotin biosynthesis homolog yhfU - Bacillus subtilis >gi|2226257|emb|CAA74544.1| (Y14084) hypothetical protein [Bacillus subtilis] >gi|2633373|emb|CAB12877.1| (Z99109) similar to biotin biosynthesis [Bacillus subtilis] Length = 186 Score = 79.2 bits (192), Expect = 3e-14 Identities = 42/112 (37%), Positives = 68/112 (60%), Gaps = 3/112 (2%) Query: 1 MKAKEVSLVALFTALTAVGA---QISISIGPVPLTLQVLFVVLAGLVLGPRLGFLSMLLY 57 +K ++ +A+FTAL AV + +S PVP+TLQ L V+LAG +L P+ FLS L++ Sbjct: 2 LKLIDMMHIAIFTALMAVLGFMPPLFLSFTPVPITLQTLGVMLAGSILRPKSAFLSQLVF 61 Query: 58 DVLGMLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGLGKGKVKYLTSL 109 +L G P+ G GG GP+ G+++A+P+AS + L +++ +T L Sbjct: 62 LLLVAFGAPLLPGGRGGFGVFFGPSAGFLIAYPLASWLISLAANRLRKVTVL 113 >sp|O26990|Y905_METTH HYPOTHETICAL PROTEIN MTH905 >gi|7482274|pir||B69221 conserved hypothetical protein MTH905 - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622001|gb|AAB85403.1| (AE000866) conserved protein [Methanobacterium thermoautotrophicum] Length = 157 Score = 71.0 bits (171), Expect = 7e-12 Identities = 47/141 (33%), Positives = 75/141 (52%), Gaps = 17/141 (12%) Query: 40 LAGLVLGPRLGFLSMLLYDVLGMLGFPVFAGFSGGIAHILGPTGGYIVAFPIASLIAGLG 99 ++G++LG G LS L+Y ++G G P FA SGG +LG TGGY++ F +A+L+ G Sbjct: 1 MSGVILGRYWGGLSQLIYVIIGAAGVPWFADMSGGPEVLLGATGGYLLGFILAALLLGHF 60 Query: 100 KGK---------VKYLTSLASLGVIYSLGWLRL--------SSFIGAEKALKVGVLPFIL 142 + + L ++A+ G+IY G + L + G + L +G+LPFI Sbjct: 61 VDRHIRARKFTPMLGLMTIANFGLIYIPGLVVLGLWSLKTQGTLPGPWELLVMGLLPFIP 120 Query: 143 PDIVKILVAIAIVNRLNIRRP 163 DI+KI A A+ + + P Sbjct: 121 GDILKITGAAALTRAITPKEP 141 >pir||D71524 hypothetical protein CT359 - Chlamydia trachomatis (serotype D, strain UW3/Cx) >gi|3328782|gb|AAC67955.1| (AE001309) hypothetical protein [Chlamydia trachomatis] Length = 196 Score = 70.6 bits (170), Expect = 1e-11 Identities = 56/153 (36%), Positives = 80/153 (51%), Gaps = 21/153 (13%) Query: 20 AQISISIG--PVPLTLQVLFVVLAGLVLGPRLGFLSMLLYDVLGMLGFPVFAGFSGGIAH 77 A+ISIS+ PVP+T Q L V GLV+ P++ +++ Y + G+ FPVF GIA Sbjct: 33 AKISISLPFTPVPITFQTLGVFCLGLVMTPQMAVTTVVAYLLEGLF-FPVFCSPMCGIAV 91 Query: 78 ILGPTGGYIVAF-PIASLIAGL--------GKGKVKYLTSLASLGVIYSLGWLRLSSF-- 126 GPT GY+ +F P +LI+ L + V + GV LG L L+ F Sbjct: 92 FCGPTAGYLFSFVPAVALISWLYGKFGRSEARSWVVAMILFVGGGVSLCLGALWLACFLK 151 Query: 127 -IGAEKAL------KVGVLPFILPDIVKILVAI 152 IG +L K+GVLPF++ IVKI + + Sbjct: 152 SIGVSNSLDLVGAFKIGVLPFLIGKIVKIALVV 184 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.328 0.148 0.424 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 53788529 Number of Sequences: 2977 Number of extensions: 2268215 Number of successful extensions: 8007 Number of sequences better than 1.0e-10: 15 Number of HSP's better than 0.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 7987 Number of HSP's gapped (non-prelim): 15 length of query: 163 length of database: 189,106,746 effective HSP length: 51 effective length of query: 112 effective length of database: 158,583,909 effective search space: 17761397808 effective search space used: 17761397808 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 40 (21.7 bits) S2: 162 (67.5 bits)