BLASTP 2.0.10 [Aug-26-1999] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= PAB2302 (moeB) DE:MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN (MOEB). (230 letters) Database: ./suso.pep; /banques/blast2/nr.pep 598,487 sequences; 189,106,746 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||D75192 molybdenum cofactor biosynthesis protein (moeb) PAB2... 459 e-128 pir||E71224 probable HESA protein - Pyrococcus horikoshii >gi|32... 422 e-117 pir||E72538 probable HESA protein APE1594 - Aeropyrum pernix (st... 178 5e-44 pir||A70415 molybdopterin biosynthesis protein MoeB - Aquifex ae... 176 3e-43 gi|11498163 thiamine biosynthesis protein (thiF) [Archaeoglobus ... 172 3e-42 gb|AAG18717.1| (AE004976) molybdenum cofactor biosynthesis prote... 167 1e-40 pir||C70921 probable molybdopterin biosynthesis protein moeB - M... 162 2e-39 sp|P46048|HEA2_ANAVA HESA PROTEIN, VEGETATIVE >gi|1075650|pir||S... 162 3e-39 gi|11498142 molybdenum cofactor biosynthesis protein (moeB) [Arc... 162 4e-39 pir||D82801 molybdopterin biosynthesis protein XF0466 [imported]... 160 1e-38 gb|AAD18050.1| (AF124159) molybdopterin synthase sulphurylase [A... 158 6e-38 emb|CAA76284.1| (Y16560) MPT-synthase sulfurylase [Synechococcus... 157 1e-37 gb|AAF52691.1| (AE003621) CG13090 gene product [Drosophila melan... 156 2e-37 pir||A49890 fdxH 5'-region hypothetical protein 1 - Plectonema b... 154 9e-37 sp|P46037|HESA_PLEBO HESA PROTEIN >gi|481924|pir||S40167 hypothe... 154 9e-37 sp|P18500|HESA_ANASP HESA PROTEIN >gi|97697|pir||S11900 hypothet... 150 2e-35 pir||F83381 probable molybdopterin biosynthesis protein MoeB PA2... 149 2e-35 emb|CAC01308.1| (AL390968) putative sulfurylase [Streptomyces co... 148 4e-35 dbj|BAB05138.1| (AP001512) molybdopterin biosynthesis [Bacillus ... 148 5e-35 pir||G70594 probable MOEB- homolog - Mycobacterium tuberculosis ... 147 1e-34 gb|AAD18052.1| (AF124161) molybdopterin synthase sulphurylase [N... 147 1e-34 gb|AAF82644.1|AF167538_11 (AF167538) HesA [Trichodesmium sp. IMS... 146 2e-34 pir||F64621 thiamin biosynthesis protein - Helicobacter pylori (... 146 2e-34 pir||H83062 molybdopterin biosynthesis MoeB protein PA4663 [impo... 146 3e-34 sp|P45211|MOEB_HAEIN MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN >gi... 145 6e-34 pir||S76179 hypothetical protein - Synechocystis sp. (strain PCC... 144 8e-34 gi|7657339 molybdopterin synthase sulfurylase [Homo sapiens] >gi... 144 1e-33 pir||F69659 molybdopterin biosynthesis protein moeB - Bacillus s... 143 1e-33 gb|AAD21552.1| (AF088896) molybdopterin biosynthesis protein [Zy... 143 2e-33 pir||H71891 molybdopterin-synthase sulfurylase - Helicobacter py... 140 2e-32 gb|AAD39225.1|AF149851_6 (AF149851) MoeB-like protein [Pseudomon... 139 3e-32 pir||F82439 molybdopterin biosynthesis MoeB protein VCA0618 [imp... 138 6e-32 pir||E69845 thiamin biosynthesis homolog yjbU - Bacillus subtili... 138 6e-32 gb|AAD21203.1| (AF128444) MoaB [Rhodobacter capsulatus] 137 1e-31 sp|Q56067|MOEB_SALTY MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN >gi... 133 2e-30 gi|11465801 ORF382 [Porphyra purpurea] >gi|1723404|sp|P51335|YCX... 130 1e-29 sp|P12282|MOEB_ECOLI MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN >gi... 130 2e-29 pir||T32638 hypothetical protein F42G8.6 - Caenorhabditis elegan... 129 2e-29 sp|Q09810|YABA_SCHPO HYPOTHETICAL 44.4 KD PROTEIN C2G11.10C IN C... 128 5e-29 gb|AAC35198.1| (AF003700) HesA [Cyanothece PCC8801] 128 5e-29 emb|CAB77681.1| (AL161416) molybdopterin synthase sulphurylase [... 128 5e-29 pir||C49695 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate... 128 7e-29 sp|P30138|THIF_ECOLI THIF PROTEIN 128 7e-29 pir||C65206 thiF protein - Escherichia coli >gi|396331|gb|AAC430... 126 2e-28 gb|AAC83136.1| (AF109295) MoeB [Staphylococcus carnosus] 126 3e-28 gb|AAF33509.1| (AF170176) 84% identity over 251 amino acids with... 125 3e-28 gb|AAC24520.1| (AF055287) molybdenum cofactor biosynthetic prote... 123 2e-27 gb|AAD17271.1| (AF119361) unknown [Frankia sp. EuIK1] 123 2e-27 gi|6321903 moeB, thiF, UBA1; Yhr111wp [Saccharomyces cerevisiae]... 122 3e-27 gi|11465494 unknown; protochlorophyllide reductase chlN chain [C... 122 4e-27 pir||B82369 thiF protein VC0063 [imported] - Vibrio cholerae (gr... 119 3e-26 pir||G69076 molybdopterin biosynthesis protein MoeB homolog - Me... 118 7e-26 gb|AAA87953.1| (U47055) HesA [Anabaena PCC7120] 116 3e-25 pir||H75294 molybdopterin biosynthesis MoeB - Deinococcus radiod... 112 3e-24 emb|CAC11973.1| (AL445065) molybdopterin biosynthesis protein mo... 109 2e-23 pir||B81010 thiF protein NMB2062 [imported] - Neisseria meningit... 108 8e-23 pir||C81953 ThiF protein NMA0373 [imported] - Neisseria meningit... 107 1e-22 gb|AAK40637.1| Thiamine biosynthesis protein related protein (th... 107 1e-22 gb|AAG31053.1|AF264948_15 (AF264948) thiamine biosynthesis prote... 90 2e-17 gb|AAD00512.2| (U81376) PaaA [Erwinia herbicola] 87 2e-16 gb|AAB37569.1| (U40566) ubiquitin activating enzyme 2 [Arabidops... 86 3e-16 pir||T39623 ubiquitin-activating enzyme e1-like - fission yeast ... 83 3e-15 dbj|BAB15587.1| (AK026904) unnamed protein product [Homo sapiens] 81 1e-14 pir||T38013 hypothetical protein SPAC1A6.10 - fission yeast (Sc... 81 1e-14 emb|CAA75816.1| (Y15895) ubiquitin activating enzyme [Drosophila... 81 1e-14 gb|AAF58910.1| (AE003832) Uba1 gene product [Drosophila melanoga... 81 1e-14 gb|AAG23434.1|AC078892_6 (AC078892) Ubiquitin activating enzyme ... 80 2e-14 pir||S48571 ubiquitin--protein ligase E1-like protein - yeast (S... 79 4e-14 gi|4507763 ubiquitin-activating enzyme E1 (A1S9T and BN75 temper... 79 5e-14 gb|AAB71466.1| (AC000098) Similar to Caenorhabditis unknown prot... 79 5e-14 dbj|BAB10670.1| (AB025630) gene_id:MPA22.7~pir||T38013~similar t... 79 5e-14 sp|Q29504|UBA1_RABIT UBIQUITIN-ACTIVATING ENZYME E1 >gi|1381183|... 78 7e-14 pir||A38564 ubiquitin--protein ligase (EC 6.3.2.19) E1 - human >... 78 9e-14 gi|6320598 similar to ubiquitin activating enzyme (E1); Uba2p [S... 76 3e-13 pir||T29201 hypothetical protein T03F1.1 - Caenorhabditis elegan... 76 3e-13 emb|CAA40809.1| (X57583) mccB [Escherichia coli] 76 5e-13 gi|6678483 ubiquitin-activating enzyme E1, Chr X [Mus musculus] ... 75 6e-13 dbj|BAA94076.1| (AB035495) ubiquitin-activating enzyme E1 [Caras... 75 6e-13 gb|AAF25197.1|AF193553_1 (AF193553) ubiquitin-like protein activ... 75 8e-13 gb|AAF50484.2| (AE003556) Uba2 gene product [Drosophila melanoga... 75 8e-13 pir||C81307 probable molybdopterin biosynthesis protein Cj1046c ... 75 1e-12 pir||I49380 ubiquitin-activating enzyme - mouse (fragment) >gi|5... 74 1e-12 gb|AAD23691.1|AC006841_24 (AC006841) putative ubiquitin activati... 74 1e-12 gb|AAF48050.1| (AE003486) CG1749 gene product [Drosophila melano... 74 1e-12 dbj|BAB04974.1| (AP001511) unknown conserved protein [Bacillus h... 74 1e-12 pir||T00587 probable ubiquitin--protein ligase (EC 6.3.2.19) - A... 74 2e-12 pir||T20004 hypothetical protein C47E12.5 - Caenorhabditis elega... 74 2e-12 gb|AAD18053.1| (AF124162) molybdopterin synthase sulphurylase [N... 73 2e-12 gi|6322639 ubiquitin activating enzyme, similar to Uba2p; Uba1p ... 73 3e-12 gi|6755923 ubiquitin activating enzyme E1, Chr Y 1 [Mus musculus... 73 3e-12 gb|AAF00149.1|AF150963_1 (AF150963) ubiquitin-activating enzyme ... 73 3e-12 gb|AAF31704.1|AF218864_1 (AF218864) Smt3 activating enzyme 2 [Dr... 73 3e-12 gb|AAG03059.1|AF288693_1 (AF288693) Ube1l [Mus musculus] 73 3e-12 emb|CAA39056.1| (X55386) ubiquitin-activating enzyme [Saccharomy... 71 9e-12 emb|CAB54319.2| (Z82062) contains similarity to Pfam domain: PF0... 71 9e-12 pir||T51083 related to ubiquitin-activating enzyme homolog UBA2 ... 71 2e-11 sp|P20973|UBA1_WHEAT UBIQUITIN-ACTIVATING ENZYME E1 1 >gi|100841... 70 2e-11 sp|P31252|UBA3_WHEAT UBIQUITIN-ACTIVATING ENZYME E1 3 >gi|743798... 70 3e-11 pir||A48195 ubiquitin--protein ligase E1 homolog - human 69 6e-11 gi|4507767 ubiquitin-activating enzyme E1-like; Ubiquitin-activa... 69 6e-11 pir||T03964 probable ubiquitin--protein ligase (EC 6.3.2.19) - c... 69 6e-11 sp|P31251|UBA2_WHEAT UBIQUITIN-ACTIVATING ENZYME E1 2 >gi|170684... 68 8e-11 >pir||D75192 molybdenum cofactor biosynthesis protein (moeb) PAB2302 - Pyrococcus abyssi (strain Orsay) >gi|5457496|emb|CAB48987.1| (AJ248283) MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN (MOEB) [Pyrococcus abyssi] Length = 230 Score = 459 bits (1168), Expect = e-128 Identities = 230/230 (100%), Positives = 230/230 (100%) Query: 1 MLSESELERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ 60 MLSESELERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ Sbjct: 1 MLSESELERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ 60 Query: 61 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD 120 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD Sbjct: 61 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD 120 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKG 180 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKG Sbjct: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKG 180 Query: 181 KFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 KFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI Sbjct: 181 KFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 >pir||E71224 probable HESA protein - Pyrococcus horikoshii >gi|3256441|dbj|BAA29124.1| (AP000001) 230aa long hypothetical HESA protein [Pyrococcus horikoshii] Length = 230 Score = 422 bits (1074), Expect = e-117 Identities = 201/230 (87%), Positives = 221/230 (95%) Query: 1 MLSESELERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ 60 MLSE ELERYDRQIM+FGVEGQEKLK+AKVAV GVGGLGSPVAYYL AAGVGR+LLID+Q Sbjct: 1 MLSERELERYDRQIMLFGVEGQEKLKRAKVAVAGVGGLGSPVAYYLVAAGVGRVLLIDDQ 60 Query: 61 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD 120 +PELSNLNRQILHWEED+G+NPKPLSAKWKLE+FNSDV++ET+VGKLTEEN+ +VL GVD Sbjct: 61 IPELSNLNRQILHWEEDVGKNPKPLSAKWKLERFNSDVRVETYVGKLTEENVNEVLDGVD 120 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKG 180 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEG+YGQVTTI+PGKTKRLR+IFP+IKK G Sbjct: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGMYGQVTTIVPGKTKRLRDIFPKIKKTG 180 Query: 181 KFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 KFPILGATAGVI SIQV EVVKLITGYGEPL NKLL+VDLANN+FEI+EI Sbjct: 181 KFPILGATAGVIASIQVAEVVKLITGYGEPLLNKLLLVDLANNIFEIVEI 230 >pir||E72538 probable HESA protein APE1594 - Aeropyrum pernix (strain K1) >gi|5105281|dbj|BAA80594.1| (AP000062) 267aa long hypothetical HESA protein [Aeropyrum pernix] Length = 267 Score = 178 bits (447), Expect = 5e-44 Identities = 99/226 (43%), Positives = 143/226 (62%), Gaps = 2/226 (0%) Query: 6 ELERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELS 65 +L Y RQ+ + GV GQ +L +KVAVVG+GGLG+ A YL A+GVGR++L+D V E S Sbjct: 19 DLNVYSRQLGLLGVRGQLRLSSSKVAVVGLGGLGNLAAAYLAASGVGRLILVDRDVVEPS 78 Query: 66 NLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDC 125 NLNRQ+L+ + D+GR K ++A +L + N + +IE L ED+ DVIVD Sbjct: 79 NLNRQVLYGKGDVGRY-KAVAAAERLGELNPEAEIEPVPEALDPALAEDLAREADVIVDG 137 Query: 126 LDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFP-RIKKKGKFPI 184 LDN+ R LD + + G PLVHGA E ++GQVTT+ G++ L I P ++ G I Sbjct: 138 LDNWMARLWLDAASWRRGKPLVHGAAERMHGQVTTVERGRSSCLACIAPSNPERAGCTTI 197 Query: 185 LGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 + T GV+ S++V E +KL+TG GEPL N+L ++DL E++ + Sbjct: 198 IAPTVGVVSSLEVLEAIKLLTGVGEPLYNRLAVIDLTTPRIEVLRL 243 >pir||A70415 molybdopterin biosynthesis protein MoeB - Aquifex aeolicus >gi|2983752|gb|AAC07312.1| (AE000735) molybdopterin biosynthesis protein MoeB [Aquifex aeolicus] Length = 271 Score = 176 bits (441), Expect = 3e-43 Identities = 104/240 (43%), Positives = 144/240 (59%), Gaps = 14/240 (5%) Query: 3 SESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ 60 +E +++RY R I++ G +GQ KL ++KV V+G GGLGSP YYL AAGVG I ++D Sbjct: 5 TEEQIKRYARHIILPEVGGKGQAKLLQSKVLVIGAGGLGSPALYYLAAAGVGTIGIVDFD 64 Query: 61 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD 120 V + SNL RQILH E +G PK SAK LE N DVK+ T+ K+ + N+ D++ D Sbjct: 65 VVDFSNLQRQILHTTERVG-TPKVESAKKTLEALNPDVKVITYNTKINKNNVMDIIKDYD 123 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKR----LREIFPRI 176 VI+D DNF TR+L++D + G PLV A+ GQ T+ + K R +FP Sbjct: 124 VILDGTDNFPTRFLINDACYFAGKPLVSAAMLRFEGQ-CTVFDFRDKENSPCYRCLFPEP 182 Query: 177 KKKGKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 G P ILG+ G++GSIQ TE +KL+ G GEPL KLLI+D + F +++ Sbjct: 183 PPPGLVPSCQEAGILGSVGGIMGSIQATEAIKLLLGIGEPLVGKLLIMDALSMDFRKVKL 242 >gi|11498163 thiamine biosynthesis protein (thiF) [Archaeoglobus fulgidus] >gi|7448806|pir||A69319 thiamin biosynthesis protein (thiF) homolog - Archaeoglobus fulgidus >gi|2650075|gb|AAB90689.1| (AE001066) thiamine biosynthesis protein (thiF) [Archaeoglobus fulgidus] Length = 267 Score = 172 bits (432), Expect = 3e-42 Identities = 100/238 (42%), Positives = 139/238 (58%), Gaps = 10/238 (4%) Query: 1 MLSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 ML++ +++RY RQI+I G GQEKL KAKV VVG GGLGSP +L AAGVGRI + D Sbjct: 1 MLNKEQVKRYGRQILIPEIGGRGQEKLLKAKVLVVGAGGLGSPAIEFLAAAGVGRIGIAD 60 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 +++NL+RQ +H +LG N K SA +EK N DV+++ + L+ EN +V+ Sbjct: 61 GDEVDITNLHRQTIH-AGNLGVN-KAESAASFVEKLNPDVEVDVYPFHLSAENAREVISK 118 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK 178 DV++DC D+F +R+L++D P VH AV GQ+ T+IP +T R P Sbjct: 119 YDVVLDCTDSFSSRFLINDACVLESKPFVHAAVLRFEGQIMTVIPHETACYRCFLPEAPP 178 Query: 179 KGKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 G P I+G T G +Q E +KLI G GE N+LL D+ +N F IE+ Sbjct: 179 PGSVPSCREAGIIGTTTAFFGVLQANEAIKLILGTGELFTNRLLYADILHNDFNFIEV 236 >gb|AAG18717.1| (AE004976) molybdenum cofactor biosynthesis protein; MoeB [Halobacterium sp. NRC-1] Length = 278 Score = 167 bits (419), Expect = 1e-40 Identities = 99/238 (41%), Positives = 133/238 (55%), Gaps = 10/238 (4%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L ++L+RY R I++ G GQ L++A V VVG GGLGSPV YL AAGVG I + D+ Sbjct: 6 LDATQLDRYSRHIIMDDVGATGQAALREAAVLVVGAGGLGSPVIQYLAAAGVGTIGIADD 65 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 ELSNL RQ +H +D+G K SA ++ N DV ++ ++T + + D++ Sbjct: 66 DAVELSNLQRQTIHGTDDVGEQ-KVDSAAAFVDTLNPDVDVQRHDQRVTADTVTDLIAAY 124 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKT-KRLREIFPRIKK 178 DV+VD DNF TRYL++D G+P HGA+ GQVTT G R +FP Sbjct: 125 DVVVDASDNFRTRYLINDACTLAGVPFSHGAIFQFEGQVTTFTGGDDGPCYRCLFPEAPP 184 Query: 179 KGKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 G P +LG G IGSIQ TE VK I G G+ L +LL D A+ FE + + Sbjct: 185 AGTVPDCATAGVLGVLPGTIGSIQATETVKHILGVGDSLDGRLLFYDAADMSFETVAL 242 >pir||C70921 probable molybdopterin biosynthesis protein moeB - Mycobacterium tuberculosis (strain H37RV) >gi|2076691|emb|CAB08373.1| (Z95150) moeB [Mycobacterium tuberculosis] Length = 389 Score = 162 bits (407), Expect = 2e-39 Identities = 97/237 (40%), Positives = 133/237 (55%), Gaps = 9/237 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L+ E+ RY R ++I GV GQ++LK A+V +G GGLGSP YL AAGVG I +ID Sbjct: 14 LTRDEVRRYSRHLIIPDIGVNGQQRLKDARVLCIGAGGLGSPALLYLAAAGVGTIGIIDG 73 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 + SNL RQI+H D+GR PK SA + + N V++ + LT +N ++ Sbjct: 74 DHVDESNLQRQIIHGTSDVGR-PKVESAAEAVAEINPHVRVTQYREMLTHDNALEIFGDH 132 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKK 179 D+IVD DNF TRYL++D A G P V G++ GQ + PG+ R + P Sbjct: 133 DLIVDGTDNFTTRYLINDAAVLAGKPYVWGSIYRFNGQTSVFWPGRGPCYRCLHPAPPPP 192 Query: 180 GKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 G P +LGA I SIQVTEV+KL+TG G PL +LL+ + + + I I Sbjct: 193 GLVPSCAEGGVLGAICATIASIQVTEVLKLLTGVGTPLVGRLLMYEALDATYHQIRI 249 >sp|P46048|HEA2_ANAVA HESA PROTEIN, VEGETATIVE >gi|1075650|pir||S49994 hypothetical protein 1 (fdxH2 5' region) - Anabaena variabilis >gi|7448802|pir||S70247 hypothetical protein 1 (fdxH2 5' region) - Anabaena variabilis >gi|599649|emb|CAA86989.1| (Z46890) unknown [Anabaena variabilis] Length = 265 Score = 162 bits (406), Expect = 3e-39 Identities = 91/228 (39%), Positives = 139/228 (60%), Gaps = 5/228 (2%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L+ +ELERY RQI++ FG E Q++LK A V V GVGGLG A YL AGVGR++L+ Sbjct: 4 LTSTELERYRRQIILPGFGQEAQQRLKSATVLVTGVGGLGGTAALYLAIAGVGRLILVRG 63 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 L ++NRQIL ++ +G+ P+ AK +LE N DV++E +T +N++ ++ Sbjct: 64 GELRLDDMNRQILMSDDWVGK-PRVFKAKKRLEDINPDVEVEAIFDYVTPDNVDSLIQSA 122 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK- 178 DV +DC NF R LL+ + P+V A++G+ +TTIIPG T L +FP + Sbjct: 123 DVALDCAHNFGERDLLNAACVRWRKPMVEAAMDGMDAYLTTIIPGVTPCLSCLFPEKPEW 182 Query: 179 -KGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVF 225 + F +LGA +G + + E +KLITG+ +PL+++LL ++L F Sbjct: 183 DRRGFGVLGAVSGTLACLTALEAMKLITGFSQPLSSELLTMNLHQLTF 230 >gi|11498142 molybdenum cofactor biosynthesis protein (moeB) [Archaeoglobus fulgidus] >gi|7448807|pir||C69316 molybdenum cofactor biosynthesis protein (moeB) homolog - Archaeoglobus fulgidus >gi|2650094|gb|AAB90707.1| (AE001068) molybdenum cofactor biosynthesis protein (moeB) [Archaeoglobus fulgidus] Length = 243 Score = 162 bits (405), Expect = 4e-39 Identities = 98/216 (45%), Positives = 131/216 (60%), Gaps = 4/216 (1%) Query: 15 MIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHW 74 MIFG EGQ +L KAKV VVG GGLGSPV YL AAGVG+I ++D E+SNL RQI+H Sbjct: 1 MIFGEEGQRRLSKAKVLVVGAGGLGSPVIAYLAAAGVGKIGIVDGDTVEVSNLQRQIIH- 59 Query: 75 EEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCLDNFETRYL 134 +LG N K SA ++K N DV+++ + +T EN+ +V+ DV+ C D+F +R+L Sbjct: 60 AGNLGEN-KAESAAEFVKKLNPDVEVDVYPFSITPENVLEVVERYDVVAGCPDSFRSRFL 118 Query: 135 LDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFPILGATAGVIGS 194 L+D + VH AV G+V T GK R P+ ++ ILGATAG G Sbjct: 119 LNDACMILKKTFVHAAVYAWEGEVAT-FTGK-PCYRCYIPKSPEESGRAILGATAGAFGC 176 Query: 195 IQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 +Q EV+K+ITG GEPL +L+ +LA F I I Sbjct: 177 LQAAEVIKIITGSGEPLIGRLIRGNLAEMEFFTINI 212 >pir||D82801 molybdopterin biosynthesis protein XF0466 [imported] - Xylella fastidiosa (strain 9a5c) >gi|9105314|gb|AAF83276.1|AE003897_1 (AE003897) molybdopterin biosynthesis protein [Xylella fastidiosa] Length = 379 Score = 160 bits (401), Expect = 1e-38 Identities = 92/236 (38%), Positives = 131/236 (54%), Gaps = 13/236 (5%) Query: 7 LERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPEL 64 +ERY R + + G GQ++L +A+V ++G GGLGSP A+YLTAAGVG + + D E Sbjct: 116 IERYARHLRLPHIGPHGQQRLAEARVLLIGAGGLGSPAAFYLTAAGVGHLRIADHDTVER 175 Query: 65 SNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVD 124 SNL RQILH + +LG PK SA +L N V++E + NIE +L VDV +D Sbjct: 176 SNLQRQILHVDAELG-VPKAASAARRLSALNPRVQVEAMQVRANSSNIEALLQDVDVAID 234 Query: 125 CLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFP- 183 DNF RYLL+D K+G PLV+GAV GQ++ G+ + + + + +P Sbjct: 235 GADNFPARYLLNDACVKMGTPLVYGAVHQFEGQISVFDAGRQRGQAPCYRCLFPEPPYPE 294 Query: 184 ---------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 +LG GVIG +Q TE +KL+ G+PL ++L D F I + Sbjct: 295 FAPSCSEAGVLGVLPGVIGMLQATEALKLLLDIGKPLRGRVLCFDALAMQFREIRL 350 >gb|AAD18050.1| (AF124159) molybdopterin synthase sulphurylase [Arabidopsis thaliana] >gi|4337042|gb|AAD18051.1| (AF124160) molybdopterin synthase sulphurylase [Arabidopsis thaliana] >gi|9758110|dbj|BAB08582.1| (AB010071) molybdopterin synthase sulphurylase [Arabidopsis thaliana] Length = 464 Score = 158 bits (395), Expect = 6e-38 Identities = 91/237 (38%), Positives = 134/237 (56%), Gaps = 9/237 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS ++ RY RQ+++ F VEGQ L K+ V V+G GGLGSP YL A GVG++ +ID Sbjct: 64 LSPDQIYRYSRQLLLPSFAVEGQSNLLKSSVLVIGAGGLGSPALLYLAACGVGQLGIIDH 123 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 V EL+N++RQI+H E +G +PK SA NS +K++ +V L N ++L Sbjct: 124 DVVELNNMHRQIIHTEAFIG-HPKVKSAAAACRSINSTIKVDEYVEALRTSNALEILSQY 182 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFP----- 174 D+IVD DN +RY++ D +G PLV GA G+ GQ+T R +FP Sbjct: 183 DIIVDATDNPPSRYMISDCCVLLGKPLVSGAALGMEGQLTVYNHNGGPCYRCLFPTPPPT 242 Query: 175 -RIKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 ++ +LG GVIG +Q E +KL + GEPL+ ++L+ D + I++I Sbjct: 243 SACQRCSDSGVLGVVPGVIGCLQALETIKLASLVGEPLSERMLLFDALSARMRIVKI 299 >emb|CAA76284.1| (Y16560) MPT-synthase sulfurylase [Synechococcus PCC7942] Length = 391 Score = 157 bits (393), Expect = 1e-37 Identities = 88/237 (37%), Positives = 138/237 (58%), Gaps = 9/237 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L++ + RY R +++ G+EGQ++LK AKV +G GGLGSP+ YL AAG+GRI ++D Sbjct: 11 LTQDDYARYSRHLILPEVGLEGQKRLKAAKVLCIGTGGLGSPLLLYLAAAGIGRIGIVDF 70 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 + + SNL RQ++H +G+ PK SAK ++ + N +++ + L+ EN +L Sbjct: 71 DIVDHSNLQRQVIHGTSWVGK-PKIESAKNRIHEINPYCQVDLYETALSSENALSILEPY 129 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKK 179 DVIVD DNF TRYL++D +G P V+G++ GQ T R+++P Sbjct: 130 DVIVDGTDNFPTRYLVNDACVLLGKPNVYGSIFRFEGQATVFNYEDGPNYRDLYPEPPPP 189 Query: 180 GKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 G P +LG G+IG IQ TE VK+I G G+ L+ +LL+ + + F +++ Sbjct: 190 GMVPSCAEGGVLGILPGIIGVIQATETVKIILGQGQTLSGRLLLYNALDMKFRELKL 246 >gb|AAF52691.1| (AE003621) CG13090 gene product [Drosophila melanogaster] Length = 453 Score = 156 bits (391), Expect = 2e-37 Identities = 95/237 (40%), Positives = 131/237 (55%), Gaps = 9/237 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L+ ++ RY RQ+++ FGV+GQ KLK + V +VG+GGLG P A YL AAG G + L+D Sbjct: 64 LTNDDIARYSRQLILPDFGVQGQLKLKNSSVLIVGLGGLGCPAAQYLAAAGCGHLGLVDY 123 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 E SN +RQILH E+ G + K SA+ L + N +I+ L N ++ G Sbjct: 124 DEVERSNFHRQILHSEDRCGMS-KAESARIALLELNPHCEIQCHSRMLYPHNAMHIIRGY 182 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFP----- 174 DV++DC DN TRYLL D + PLV G+ + GQ+T R IFP Sbjct: 183 DVVLDCTDNVPTRYLLSDACVMLSKPLVSGSALKMDGQLTVYNYANGPCYRCIFPVPPPP 242 Query: 175 -RIKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 + G +LGA G IG++Q E +K+I G G+ LA +LLI D + VF I I Sbjct: 243 EAVTNCGDGGVLGAVTGTIGAMQALEAIKVIVGMGDVLAGRLLIFDGGSGVFRNIRI 299 >pir||A49890 fdxH 5'-region hypothetical protein 1 - Plectonema boryanum (PCC 73110) Length = 277 Score = 154 bits (385), Expect = 9e-37 Identities = 82/228 (35%), Positives = 135/228 (58%), Gaps = 5/228 (2%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L+ +ELERY RQ+M+ F + Q +LK V GVGGLG A YL AG+G+++L+ Sbjct: 4 LTPTELERYRRQMMLPGFDEDAQRRLKSFTALVTGVGGLGGTAALYLAVAGIGKLILVRG 63 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 L ++NRQ+L + +G+ P+ AK LEK N DV++E +T +N++ ++ Sbjct: 64 GELRLDDMNRQVLMTHDTVGQ-PRVFKAKETLEKINPDVEVEAVFDYITPDNVDHLVRST 122 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK- 178 D+ +DC NF R LL++ + P+V A++G+ +TTI+PG+T L +FP + Sbjct: 123 DITLDCAHNFAERDLLNEACVRYRKPMVEAAMDGMEAYLTTILPGQTGCLTCLFPEKPEW 182 Query: 179 -KGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVF 225 + F ++GA +G + + E +KLITG+ +PL ++LL ++L F Sbjct: 183 DRRGFAVVGAVSGTLACLAALEAIKLITGFEKPLVSELLTMNLGRLEF 230 >sp|P46037|HESA_PLEBO HESA PROTEIN >gi|481924|pir||S40167 hypothetical protein 1 - Plectonema boryanum >gi|581441|emb|CAA50696.1| (X71865) ORF1 [Plectonema boryanum] Length = 277 Score = 154 bits (385), Expect = 9e-37 Identities = 82/228 (35%), Positives = 135/228 (58%), Gaps = 5/228 (2%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L+ +ELERY RQ+M+ F + Q +LK V GVGGLG A YL AG+G+++L+ Sbjct: 4 LTPTELERYRRQMMLPGFDEDAQRRLKSFTALVTGVGGLGGTAALYLAVAGIGKLILVRG 63 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 L ++NRQ+L + +G+ P+ AK LEK N DV++E +T +N++ ++ Sbjct: 64 GELRLDDMNRQVLMTHDTVGQ-PRVFKAKETLEKINPDVEVEAVFDYITPDNVDHLVRST 122 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK- 178 D+ +DC NF R LL++ + P+V A++G+ +TTI+PG+T L +FP + Sbjct: 123 DITLDCAHNFAERDLLNEACVRYRKPMVEAAMDGMEAYLTTILPGQTGCLTCLFPEKPEW 182 Query: 179 -KGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVF 225 + F ++GA +G + + E +KLITG+ +PL ++LL ++L F Sbjct: 183 DRRGFAVVGAVSGTLACLAALEAIKLITGFEKPLVSELLTMNLGRLEF 230 >sp|P18500|HESA_ANASP HESA PROTEIN >gi|97697|pir||S11900 hypothetical protein 1 - Anabaena sp >gi|39032|emb|CAA33558.1| (X15553) hesA product (AA 1-266) [Anabaena sp.] Length = 266 Score = 150 bits (374), Expect = 2e-35 Identities = 81/206 (39%), Positives = 122/206 (58%), Gaps = 3/206 (1%) Query: 17 FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEE 76 FG Q++LK A V V GVGGLG A YL AGVGR++L+ L ++NRQ+L ++ Sbjct: 6 FGEAAQKRLKSATVLVTGVGGLGGTAALYLAVAGVGRLILVRGGDLRLDDMNRQVLMTDD 65 Query: 77 DLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCLDNFETRYLLD 136 +G+ P+ AK L+ N D++IET +T EN++ ++ D+ +DC NF R LL+ Sbjct: 66 WVGK-PRVFKAKETLQAINPDIQIETIHDYITSENVDSLVQSADMALDCAHNFTERDLLN 124 Query: 137 DYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIK--KKGKFPILGATAGVIGS 194 + P+V A++G+ +TTIIPG T L IFP + F +LGA +G + Sbjct: 125 SACVRWRKPMVEAAMDGMEAYLTTIIPGVTPCLSCIFPEKPDWDRRGFSVLGAVSGTLAC 184 Query: 195 IQVTEVVKLITGYGEPLANKLLIVDL 220 + E +KLITG+ +PL ++LL +DL Sbjct: 185 LTALEAIKLITGFSQPLLSQLLTIDL 210 >pir||F83381 probable molybdopterin biosynthesis protein MoeB PA2103 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9948117|gb|AAG05491.1|AE004638_1 (AE004638) probable molybdopterin biosynthesis protein MoeB [Pseudomonas aeruginosa] Length = 392 Score = 149 bits (373), Expect = 2e-35 Identities = 88/226 (38%), Positives = 130/226 (56%), Gaps = 9/226 (3%) Query: 2 LSESELERYDRQIMIFGV--EGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS+ E++RY R I + GV EGQ++LK+A+V ++G GGLGSP YL AAGVG I L+D Sbjct: 13 LSDEEVQRYVRHITLPGVGREGQQRLKQARVLIIGTGGLGSPACLYLAAAGVGTIGLVDF 72 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 V E SNL RQ++H LG PK SA+ +++ N +++ T + +N + ++ Sbjct: 73 DVVESSNLQRQVVHGYSTLGM-PKVESAQRRMQDLNPYIEVVTHRYAIDADNAQSLIQSY 131 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKK 179 D+++D DNF TRYL++ K+G PLV GA+ GQV+ + +FP Sbjct: 132 DLVLDGTDNFATRYLVNAVCAKLGKPLVFGAIYRFEGQVSVFNLEGGPCYQCLFPNNPPP 191 Query: 180 GKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 P ++G GVIG +Q TE VK + G G+ L+ +LL D Sbjct: 192 EMAPSCNAGGVIGVLPGVIGLLQATEAVKALVGLGKSLSGRLLRFD 237 >emb|CAC01308.1| (AL390968) putative sulfurylase [Streptomyces coelicolor A3(2)] Length = 392 Score = 148 bits (371), Expect = 4e-35 Identities = 87/237 (36%), Positives = 129/237 (53%), Gaps = 9/237 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L+ E+ RY R ++I G++GQ++LK AKV VG GGLGSP YL AAGVG + +I+ Sbjct: 13 LTVDEVRRYSRHLIIPDVGMDGQKRLKNAKVLAVGAGGLGSPTLMYLAAAGVGTLGIIEF 72 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 + SNL RQ++H + D+GR PK SA+ ++ N V + +L +N+ D+ Sbjct: 73 DEVDESNLQRQVIHSQADIGR-PKAESARDTIQGINPLVDVILHQERLEADNVMDIFSQY 131 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKK 179 D+IVD DNF TRYL++D + P V G++ GQ + R ++P Sbjct: 132 DLIVDGTDNFATRYLVNDACVLLNKPYVWGSIYRFDGQASVFWSEHGPCYRCLYPEPPPP 191 Query: 180 GKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 G P +LG IGSIQ E +KL+ G GEPL +L+I D + +++ Sbjct: 192 GMVPSCAEGGVLGVLCASIGSIQTNEAIKLLQGIGEPLVGRLMIYDALEMTYRQVKV 248 >dbj|BAB05138.1| (AP001512) molybdopterin biosynthesis [Bacillus halodurans] Length = 340 Score = 148 bits (370), Expect = 5e-35 Identities = 78/223 (34%), Positives = 128/223 (56%), Gaps = 7/223 (3%) Query: 8 ERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELS 65 ERY RQ++ G EGQ+KL+ + V +VG+G LG+ +A + AG+G + ++D E S Sbjct: 7 ERYSRQMLFAPIGKEGQQKLQNSAVLIVGIGALGTVLANHFVRAGIGHVRMVDRDYVEAS 66 Query: 66 NLNRQILHWEEDLGRN-PKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVD 124 NL RQ+L E D+ PK ++A+ KL+K NSD+K+E V +T ENI +++ G+D+++D Sbjct: 67 NLQRQLLFDENDVRECLPKAVAAQQKLQKVNSDIKVEGIVADVTVENIHELMEGMDLVLD 126 Query: 125 CLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGK--- 181 DNF+TR+L++D ++G+P ++G G IP KT LR + G+ Sbjct: 127 GTDNFQTRFLINDACFQMGLPFIYGGAVSSRGMTAMFIPEKTPCLRCFIQEGQGSGQTCD 186 Query: 182 -FPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANN 223 +LG ++ S Q E +K +T + + L+ D+ N Sbjct: 187 TVGVLGPVVDIVASFQAIEALKYLTDNEQAYRHSLMSFDVWRN 229 >pir||G70594 probable MOEB- homolog - Mycobacterium tuberculosis (strain H37RV) >gi|2072679|emb|CAB08310.1| (Z95120) moeZ [Mycobacterium tuberculosis] Length = 392 Score = 147 bits (367), Expect = 1e-34 Identities = 89/241 (36%), Positives = 129/241 (52%), Gaps = 13/241 (5%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS E+ RY R ++I GV+GQ++LK A+V V+G GGLG+P YL AAGVG I ++D Sbjct: 15 LSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLYLAAAGVGTIGIVDF 74 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 V + SNL RQ++H D+GR+ K SA+ + N +++ +L N D+ Sbjct: 75 DVVDESNLQRQVIHGVADVGRS-KAQSARDSIVAINPLIRVRLHELRLAPSNAVDLFKQY 133 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTI----IPGKTKRLREIFPR 175 D+I+D DNF TRYL++D A G P V G++ GQ + G R+++P Sbjct: 134 DLILDGTDNFATRYLVNDAAVLAGKPYVWGSIYRFEGQASVFWEDAPDGLGVNYRDLYPE 193 Query: 176 IKKKGKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIE 229 G P +LG + S+ TE +KLITG GE L +LL+ D + I Sbjct: 194 PPPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLLVYDALEMSYRTIT 253 Query: 230 I 230 I Sbjct: 254 I 254 >gb|AAD18052.1| (AF124161) molybdopterin synthase sulphurylase [Nicotiana plumbaginifolia] Length = 401 Score = 147 bits (367), Expect = 1e-34 Identities = 87/237 (36%), Positives = 128/237 (53%), Gaps = 9/237 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS + RY R +++ FGV+GQ L K+ V V+G GGLGSP YL A GVGRI ++D Sbjct: 3 LSPDMIYRYSRHLLLPSFGVQGQANLLKSSVLVIGAGGLGSPALLYLAACGVGRIGIVDH 62 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 V EL+NL RQI+H E +G++ K SA NS +I N +++ Sbjct: 63 DVVELNNLQRQIIHTEAYIGKS-KVKSAAAACRSINSSTQIVEHKEAFRTSNALEIVSKY 121 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFP----- 174 DV+VD DN +RY+++D +G PL+ GA GL GQ+T R +FP Sbjct: 122 DVVVDATDNAPSRYMINDCCFVLGKPLISGAALGLEGQLTVYNYNGGPCYRCLFPTPPPT 181 Query: 175 -RIKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 ++ +LG GVIG +Q E +K+ + GEPL+ ++L+ D + I++I Sbjct: 182 NACQRCADSGVLGVVPGVIGCLQALEAIKVASLVGEPLSGRMLLFDALSGRIRIVKI 238 >gb|AAF82644.1|AF167538_11 (AF167538) HesA [Trichodesmium sp. IMS101] Length = 273 Score = 146 bits (366), Expect = 2e-34 Identities = 83/228 (36%), Positives = 132/228 (57%), Gaps = 5/228 (2%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L+ ELERY RQ+M+ FG E Q++LK V GVGGLG VA YL AG+G+++L+ Sbjct: 5 LTPKELERYSRQMMLPGFGEEAQQRLKSTTAVVTGVGGLGGTVALYLAVAGIGKLILVRG 64 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 L ++NRQIL ++ +G+ + AK L N D++IE +T EN++ ++ Sbjct: 65 GELRLDDMNRQILMTDDWVGK-VRVDKAKETLSAINPDIEIEAVPEYVTAENVDPLVQSA 123 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK- 178 D+ +DC NF+ R LL+ + P+V A+ + +TTI PGKT L +FP + Sbjct: 124 DIAIDCAHNFDERNLLNAACVRWRKPMVEAAMNSMEAYLTTIDPGKTPCLSCLFPEKPQW 183 Query: 179 -KGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVF 225 + F +LG +G + + +E +K ITG+ +PL ++LL ++L + F Sbjct: 184 DRRGFGVLGIVSGTLACLAASEAIKQITGFSKPLWSQLLTMNLDSMEF 231 >pir||F64621 thiamin biosynthesis protein - Helicobacter pylori (strain 26695) >gi|2313946|gb|AAD07863.1| (AE000593) thiamin biosynthesis protein (thiF) [Helicobacter pylori 26695] Length = 255 Score = 146 bits (365), Expect = 2e-34 Identities = 91/234 (38%), Positives = 132/234 (55%), Gaps = 7/234 (2%) Query: 1 MLSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 MLS E ERY R IM+ G EGQ KL K+ V V+G GGLGS V YL AAG+G+I ++D Sbjct: 1 MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVIGAGGLGSAVLMYLCAAGIGKIGIVD 60 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 V ++SNL RQI+H ++ L ++ K SAK +L++ N+ ++IE F + N ++ Sbjct: 61 FDVVDMSNLQRQIIHSQDFLNQS-KASSAKARLKQLNAGIEIEAFEERFKAHNALSLIEP 119 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK 178 D I+D DNF ++L++D P H V GQ +++P + L +F + K Sbjct: 120 YDFIIDATDNFNAKFLINDACVLAQKPYSHAGVLEYRGQSMSVLP-HSACLACVFDKPPK 178 Query: 179 KGKFPI---LGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIE 229 KG PI G GV+G IQ +E +K G+ L N LLI D+ F+ I+ Sbjct: 179 KGLNPISGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQ 232 >pir||H83062 molybdopterin biosynthesis MoeB protein PA4663 [imported] - Pseudomonas aeruginosa (strain PAO1) >gi|9950918|gb|AAG08050.1|AE004880_6 (AE004880) molybdopterin biosynthesis MoeB protein [Pseudomonas aeruginosa] Length = 252 Score = 146 bits (364), Expect = 3e-34 Identities = 86/240 (35%), Positives = 136/240 (55%), Gaps = 13/240 (5%) Query: 1 MLSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 MLS+ EL RY RQI++ VEGQ +LK+++ +VG+GGLGSPVA YL AAGVG + L D Sbjct: 1 MLSDEELLRYSRQILLAQVDVEGQLRLKRSRALIVGLGGLGSPVALYLAAAGVGELHLAD 60 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 +L+NL RQ++H +G K SA +L N +++ + L E++++ + Sbjct: 61 FDTVDLTNLQRQVIHDGASVGIG-KVESAMRRLGALNPQIRLVSHSAALDEDSLDAAVAA 119 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK 178 VD+++DC DNF TR ++ + G PLV GA L GQ++ + + + + Sbjct: 120 VDLVLDCTDNFATREAVNVACVRAGKPLVSGAAIRLEGQLSVFDTRRDE--SPCYHCLYG 177 Query: 179 KG--------KFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 G + ++G G++GS+Q E +KL+ G+GEPL +LL+VD F + + Sbjct: 178 HGSEAELTCSEAGVIGPLVGLVGSLQALEALKLLAGFGEPLVGRLLLVDALGTRFRELRV 237 >sp|P45211|MOEB_HAEIN MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN >gi|1075035|pir||C64124 molybdopterin biosynthesis protein moeB - Haemophilus influenzae (strain Rd KW20) >gi|1574288|gb|AAC23099.1| (U32823) molybdopterin biosynthesis protein (moeB) [Haemophilus influenzae Rd] Length = 243 Score = 145 bits (361), Expect = 6e-34 Identities = 88/234 (37%), Positives = 132/234 (55%), Gaps = 9/234 (3%) Query: 2 LSESELERYDRQIMIFGVE--GQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS E RY+RQI++ V+ GQEKLK +K+ +VG+GGLG + YL AAGVG + L+D Sbjct: 4 LSHEEELRYNRQIILKSVDFDGQEKLKASKMLIVGLGGLGCAASQYLAAAGVGNLTLLDF 63 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 LSNL RQ+LH + L PK SAK LE+ N + IET KL EE + +++ Sbjct: 64 DTVSLSNLQRQVLHCDARLNM-PKVESAKIALEQINPHINIETINAKLDEEKLAEIIPHF 122 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTI-IPGKTKRLREIFPRIKK 178 D+++DC DN E R LD + + +PL+ GA + GQV+ T R++ ++ Sbjct: 123 DIVLDCTDNVEIRNQLDRQCNHMKVPLISGAAIRMEGQVSVFTYEPNTPTYRDLSKLFRQ 182 Query: 179 K----GKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD-LANNVFEI 227 + +L G++G IQ E +K+ G+ L +LL++D + N+ EI Sbjct: 183 NVLSCVEAGVLAPIVGIVGCIQALEAIKVRLKIGKNLCGRLLMIDGFSMNIREI 236 >pir||S76179 hypothetical protein - Synechocystis sp. (strain PCC 6803) >gi|1653525|dbj|BAA18438.1| (D90914) molybdopterin biosynthesis MoeB protein [Synechocystis sp.] Length = 392 Score = 144 bits (360), Expect = 8e-34 Identities = 80/237 (33%), Positives = 135/237 (56%), Gaps = 9/237 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS+ + +RY R I++ G++GQ+++K A V +G GGLG+P+ YL AAGVGRI L+D Sbjct: 11 LSQDDYQRYSRHIILPEVGLDGQKRIKAASVLCIGSGGLGAPLLLYLAAAGVGRIGLVDF 70 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 + + SNL RQI+H +G+ PK SAK + + N +++ + +++ N D++ Sbjct: 71 DIVDTSNLQRQIIHGTSWVGK-PKIESAKQSIMEINPFCQVDLYETQISSANALDIIAPY 129 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKK 179 D+++D DNF TRYL +D + P V+G++ GQ T R+++P Sbjct: 130 DIVIDGTDNFPTRYLTNDACVLLNKPNVYGSIFRFEGQATVFNYQGGPNYRDLYPEPPPP 189 Query: 180 GKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 G P +LG G++G+IQ TE +K+I G L+ +LL+ + + F +++ Sbjct: 190 GMVPSCSEGGVLGILPGIVGTIQATEALKIILGAPNTLSGRLLLFNAWDMKFRELKL 246 >gi|7657339 molybdopterin synthase sulfurylase [Homo sapiens] >gi|11420522|ref|XP_009466.1| molybdopterin synthase sulfurylase [Homo sapiens] >gi|3851719|gb|AAC72412.1| (AF102544) molybdopterin synthase sulfurylase [Homo sapiens] >gi|5817252|emb|CAB53750.1| (AL034553) dJ914P20.3 (molybdopterin synthase sulfurylase (MOCS3)) [Homo sapiens] Length = 460 Score = 144 bits (359), Expect = 1e-33 Identities = 91/237 (38%), Positives = 122/237 (51%), Gaps = 9/237 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS E+ RY RQ+++ GV GQ +L A V +VG GGLG P+A YL AAGVGR+ L+D Sbjct: 55 LSRDEILRYSRQLVLPELGVHGQLRLGTACVLIVGCGGLGCPLAQYLAAAGVGRLGLVDY 114 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 V E+SNL RQ+LH E L K SA L + NS V+ + LT D++ Sbjct: 115 DVVEMSNLARQVLHGEA-LAGQAKAFSAAASLRRLNSAVECVPYTQALTPATALDLVRRY 173 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPR---- 175 DV+ DC DN TRYL++D G PLV + GQ+T R IFP+ Sbjct: 174 DVVADCSDNVPTRYLVNDACVLAGRPLVSASALRFEGQITVYHYDGGPCYRCIFPQPPPA 233 Query: 176 --IKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 + +LG GV+G +Q EV+K+ G G + LL+ D F I + Sbjct: 234 ETVTNCADGGVLGVVTGVLGCLQALEVLKIAAGLGPSYSGSLLLFDALRGHFRSIRL 290 >pir||F69659 molybdopterin biosynthesis protein moeB - Bacillus subtilis >gi|2633798|emb|CAB13300.1| (Z99111) molybdopterin biosynthesis protein [Bacillus subtilis] >gi|3282111|gb|AAC24901.1| (AF012285) molybdopterin biosynthesis protein MoeB [Bacillus subtilis] Length = 339 Score = 143 bits (358), Expect = 1e-33 Identities = 92/229 (40%), Positives = 126/229 (54%), Gaps = 19/229 (8%) Query: 8 ERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELS 65 ERY RQI G EGQ++L + V +VG G LG+ A L+ AGVG I +ID E S Sbjct: 3 ERYSRQIRFKQIGEEGQKRLADSHVLIVGAGALGTAGAEGLSRAGVGTITIIDRDYVEWS 62 Query: 66 NLNRQILHWEEDLG-RNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVD 124 NL RQ L+ E D R PK ++AK L NS++ IE +V + T E +E ++ DV++D Sbjct: 63 NLQRQQLYTESDAKLRMPKAMAAKEHLSAINSEIHIEAYVTEGTAETLEPLIEKADVVID 122 Query: 125 CLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFPI 184 DNFETR L++D A K P V+GA G T+IPG+T L +F +I P+ Sbjct: 123 ATDNFETRMLINDLAQKTKTPWVYGACVSSQGMFMTVIPGETPCLSCLFEQI------PV 176 Query: 185 LGA---TAGVI-------GSIQVTEVVKLITGYGEPLANKLLIVDLANN 223 GA TAG+I + Q E +KL+TG E + + D+ NN Sbjct: 177 GGATCDTAGIIPPAVHIVSAYQQAEALKLLTGQKEAIQRGFVTFDVWNN 225 >gb|AAD21552.1| (AF088896) molybdopterin biosynthesis protein [Zymomonas mobilis] Length = 251 Score = 143 bits (357), Expect = 2e-33 Identities = 86/239 (35%), Positives = 134/239 (55%), Gaps = 11/239 (4%) Query: 1 MLSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 +L++++L+RY R I++ G +GQ+KL A VA+VG GG+GSPV YL AAGVGR+ ++D Sbjct: 2 ILTDNQLDRYARHIVLPEIGGKGQKKLLSAHVAIVGAGGIGSPVIQYLAAAGVGRLTIVD 61 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 LSNL RQ L D+G + ++A +++ N DVK+ + KL EN + ++ Sbjct: 62 NDEVSLSNLQRQTLFATRDIGAHKVAMAAN-VVQRLNPDVKVLPYDQKLDAENAKKLIGQ 120 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIF----- 173 D+IVD DNF TR ++ D A ++ IPLV AV Q+ + + F Sbjct: 121 ADIIVDGSDNFGTRLVVSDVATELKIPLVSAAVIRFEAQLAVFKGYEASKPYRCFVGDDP 180 Query: 174 --PRIKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 P + + +LGA G IGS+ E ++LIT +G +KLL++D + F + + Sbjct: 181 GEPEMTCSSQ-GVLGALTGTIGSLAAIETIRLITRFGSESGDKLLLIDAKDFRFRTVSL 238 >pir||H71891 molybdopterin-synthase sulfurylase - Helicobacter pylori (strain J99) >gi|4155314|gb|AAD06329.1| (AE001506) MOLYBDOPTERIN-SYNTHASE SULFURYLASE [Helicobacter pylori J99] Length = 255 Score = 140 bits (349), Expect = 2e-32 Identities = 87/234 (37%), Positives = 129/234 (54%), Gaps = 7/234 (2%) Query: 1 MLSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 MLS + ERY R IM+ G EGQ KL K+ V V+G GGLGS V YL AG+G+I ++D Sbjct: 1 MLSRLDKERYLRHIMLEDVGEEGQLKLLKSSVLVIGAGGLGSAVLMYLCTAGIGKIGIVD 60 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 ++SNL RQI+H +D + K SAK +L++ N+ +++ETF + N ++ Sbjct: 61 FDSVDMSNLKRQIIH-SQDCLNHSKASSAKARLKQLNAGIEVETFEERFKAHNALPLIEP 119 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK 178 D I+D DNF ++L++D P H V GQ +++P + L +F + K Sbjct: 120 YDFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSMSVLP-HSACLACVFDKPPK 178 Query: 179 KGKFP---ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIE 229 KG P + G GV+G IQ +E +K G+ L N LLI D+ F+ I+ Sbjct: 179 KGFNPTSGLWGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQ 232 >gb|AAD39225.1|AF149851_6 (AF149851) MoeB-like protein [Pseudomonas stutzeri KC] >gi|6959513|gb|AAF33130.1|AF196567_6 (AF196567) putative sulfurylase [Pseudomonas stutzeri] Length = 391 Score = 139 bits (346), Expect = 3e-32 Identities = 87/230 (37%), Positives = 123/230 (52%), Gaps = 13/230 (5%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS +E+ RY R ++I G+ GQ +LK AKV V+G GGLGSP YL AAGVG I +ID Sbjct: 13 LSRAEINRYSRHLLIPDVGMIGQRRLKNAKVLVIGAGGLGSPTLLYLAAAGVGTIGIIDF 72 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 + SNL RQ++H + +G K SAK + + N V++ET+ +L + ++ Sbjct: 73 DRVDDSNLQRQVIHGVDTVG-ELKVDSAKKAIARLNPFVQVETYTDRLERDMAIELFSRY 131 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRL----REIFPR 175 D+I+D DNF TRYL++D P V G++ GQ + L R+++P Sbjct: 132 DLIMDGTDNFATRYLVNDACVLANKPYVWGSIFRFEGQASVFWENAPNDLGLNYRDLYPE 191 Query: 176 IKKKGKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 P + G I SI TE VKLITG GEPL +L++ D Sbjct: 192 PPPPEMAPSCSEGGVFGILCASIASIMATEAVKLITGIGEPLLGRLVVYD 241 >pir||F82439 molybdopterin biosynthesis MoeB protein VCA0618 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9658030|gb|AAF96519.1| (AE004391) molybdopterin biosynthesis MoeB protein [Vibrio cholerae] Length = 254 Score = 138 bits (344), Expect = 6e-32 Identities = 84/227 (37%), Positives = 131/227 (57%), Gaps = 9/227 (3%) Query: 1 MLSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 +LS++E+ RY+RQI++ F EGQEKLK++ V ++G GGLG YL AG+G I L+D Sbjct: 5 ILSDAEMLRYNRQIILKAFDFEGQEKLKQSTVLILGAGGLGCASGQYLATAGIGHITLVD 64 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 + V ELSNL RQ+LH + D+GR K SA L N +++ET +L+++ ++ ++ Sbjct: 65 DDVVELSNLQRQVLHHDADIGR-AKVDSAADSLRLLNPHLQVETIQARLSDDELDALIAR 123 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTII---PGKT--KRLREIF 173 D+++D DN TR L+ K PLV GA + GQV+ P + + L +F Sbjct: 124 HDLVLDACDNVGTRNQLNRLCFKHKTPLVSGAAIRMEGQVSVFTYQDPAQPCYQCLSALF 183 Query: 174 -PRIKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 + I+ G+IG++Q E +K++T G P K+LI+D Sbjct: 184 GSSALSCVEAGIMAPVVGIIGAVQAMETIKVLTELGTPKQGKILILD 230 >pir||E69845 thiamin biosynthesis homolog yjbU - Bacillus subtilis >gi|2633524|emb|CAB13027.1| (Z99110) similar to thiamin biosynthesis [Bacillus subtilis] Length = 336 Score = 138 bits (344), Expect = 6e-32 Identities = 83/211 (39%), Positives = 117/211 (55%), Gaps = 19/211 (9%) Query: 9 RYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSN 66 RY RQ + G GQ+KLK+A+ ++G G LG+ A L AGVG + + D E SN Sbjct: 4 RYSRQELFAPIGPSGQKKLKEARAVIIGAGALGTASAEMLVRAGVGSVKIADRDYVEWSN 63 Query: 67 LNRQILHWEEDLGRN-PKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDC 125 L RQ L+ E+D+ + PK +A+ +L NSDV + V +T ENI +++ +IVD Sbjct: 64 LQRQQLYTEDDVKKEMPKAAAAERRLRSINSDVDVTGLVMDVTAENIFELIRDASIIVDA 123 Query: 126 LDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFPIL 185 DNFETR +++D A K GIP ++GA G YG T++PG T L + + PI Sbjct: 124 ADNFETRLIVNDAAVKEGIPFLYGACVGSYGLTFTVVPGSTPCLHCLLDAL------PIG 177 Query: 186 GATAGVIGSI----------QVTEVVKLITG 206 GAT G I QVT+ +KL+TG Sbjct: 178 GATCDTAGIISPAVLQVAVFQVTDALKLLTG 208 >gb|AAD21203.1| (AF128444) MoaB [Rhodobacter capsulatus] Length = 253 Score = 137 bits (342), Expect = 1e-31 Identities = 82/220 (37%), Positives = 123/220 (55%), Gaps = 14/220 (6%) Query: 9 RYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSN 66 RY R +++ G GQ +L A V V+G GGLGSPV YL AAG+GR+ L D+ V LSN Sbjct: 11 RYARHLILPEIGPVGQARLNAASVLVIGAGGLGSPVLLYLAAAGIGRLALADDDVVSLSN 70 Query: 67 LNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCL 126 L RQ+LH LG++ K SA+ +L N +V+IET + E ++ G D+++DC Sbjct: 71 LQRQVLHGTARLGQS-KTASARARLADLNPEVQIETHAIRFGVETAV-LVAGFDLVIDCT 128 Query: 127 DNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGK-------TKRLREIFPRIKKK 179 DN R+L++ G+PL+ GA+ GQ+ PG+ T + P + K Sbjct: 129 DNLAARHLINRACVAAGVPLLSGAIAQWEGQIGLYAPGRGGACYACTFPEPDHLPPAQSK 188 Query: 180 GKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 G ++GA GV+G+ E +K +TG G+ L +L ++D Sbjct: 189 G---VVGALPGVVGARMALEAIKHLTGAGQSLLGRLWMID 225 >sp|Q56067|MOEB_SALTY MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN >gi|1256835|gb|AAA96530.1| (U53178) moeB gene product [Salmonella typhimurium] Length = 249 Score = 133 bits (332), Expect = 2e-30 Identities = 78/225 (34%), Positives = 123/225 (54%), Gaps = 8/225 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS+ E+ RY+RQI++ F EGQE LK A+V VV +GGLG YL AGVG++ L+D Sbjct: 4 LSDQEILRYNRQIILRGFDFEGQEALKDARVLVVRLGGLGCAATQYLAGAGVGQLTLLDF 63 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 +SNL RQ LH + +G+ PK SA+ L + N + I +L ++ + ++ G Sbjct: 64 DTVSVSNLQRQTLHSDATVGQ-PKVESARDALARINPHITITPVNARLDDDAMTALIAGH 122 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKK 179 +++DC DN R L+ + +PL+ GA + GQVT +T+ R+ + Sbjct: 123 SLVLDCTDNVSVRNQLNAGCYTAKVPLISGAAIRMEGQVTVFTYRETEPCYRCLSRLFGE 182 Query: 180 GKFP-----ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 ++ GVIGS+Q E +KL+ YG+P + K+++ D Sbjct: 183 NALTCVEAGVMAPLIGVIGSLQAMEAIKLLAHYGQPASEKIVMYD 227 >gi|11465801 ORF382 [Porphyra purpurea] >gi|1723404|sp|P51335|YCXH_PORPU HYPOTHETICAL 43.4 KD PROTEIN IN RPL9-RPL11 INTERGENIC REGION (ORF382) >gi|2147563|pir||S73256 hypothetical protein 382 - red alga (Porphyra purpurea) chloroplast >gi|1276801|gb|AAC08221.1| (U38804) ORF382 [Porphyra purpurea] Length = 382 Score = 130 bits (324), Expect = 1e-29 Identities = 76/237 (32%), Positives = 129/237 (54%), Gaps = 10/237 (4%) Query: 3 SESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ 60 S E RY + +++ +EGQE+LK++ + VG GGLGSP YL A+G+G+I ++D Sbjct: 12 SLEEYTRYSKHLILPQIKLEGQERLKQSSILCVGAGGLGSPALIYLAASGIGKIGIVDND 71 Query: 61 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD 120 + ++SNL RQIL+ D+G + K AK K+ + N ++ F +L N +++ D Sbjct: 72 IIDISNLQRQILYTVNDIGLS-KAYIAKKKILEINPTCNVQIFNTRLQSINAIEIIRQYD 130 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKK- 179 +I+D DNF +RY++ D ++ ++GA+ GQV+T + R+ I+ + Sbjct: 131 IIIDGTDNFGSRYIISDSCLELNKIHIYGAIFQFEGQVSTFNYQGGPKYRDFHNNIETEN 190 Query: 180 ------GKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 +LG G+IG++Q TE +K+I GY L+ +L + FE +I Sbjct: 191 NPEDTCSNAGVLGLLPGIIGTLQATEAIKIILGYKSVLSGIILKYNAMTISFEKFKI 247 >sp|P12282|MOEB_ECOLI MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN >gi|78706|pir||B32352 molybdopterin biosynthesis protein moeB - Escherichia coli >gi|145540|gb|AAA23580.1| (M21151) chlN protein [Escherichia coli] >gi|1651375|dbj|BAA35514.1| (D90720) Molybdopterin biosynthesis MoeB protein. [Escherichia coli] >gi|1651378|dbj|BAA35521.1| (D90721) Molybdopterin biosynthesis MoeB protein. [Escherichia coli] >gi|1787048|gb|AAC73913.1| (AE000185) molybdopterin biosynthesis [Escherichia coli K12] Length = 249 Score = 130 bits (323), Expect = 2e-29 Identities = 76/225 (33%), Positives = 121/225 (53%), Gaps = 8/225 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS+ E+ RY+RQI++ F +GQE LK ++V +VG+GGLG + YL +AGVG + L+D Sbjct: 4 LSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGCAASQYLASAGVGNLTLLDF 63 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 LSNL RQ LH + +G+ PK SA+ L + N + I L + + ++ Sbjct: 64 DTVSLSNLQRQTLHSDATVGQ-PKVESARDALTRINPHIAITPVNALLDDAELAALIAEH 122 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKK 179 D+++DC DN R L+ +PLV GA + GQ+T + R+ + Sbjct: 123 DLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQITVFTYQDGEPCYRCLSRLFGE 182 Query: 180 GKFP-----ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 ++ GVIGS+Q E +K++ GYG+P + K+++ D Sbjct: 183 NALTCVEAGVMAPLIGVIGSLQAMEAIKMLAGYGKPASGKIVMYD 227 >pir||T32638 hypothetical protein F42G8.6 - Caenorhabditis elegans >gi|2702447|gb|AAB92067.1| (AF038618) contains similarity to the HESA/MOEB/THIF family [Caenorhabditis elegans] Length = 353 Score = 129 bits (322), Expect = 2e-29 Identities = 81/228 (35%), Positives = 124/228 (53%), Gaps = 12/228 (5%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 +S+ + RY RQ+++ FGV GQ+ LK V +VG GGLG PVA YL AAG+G I ++D Sbjct: 10 ISKKDAGRYSRQLLVDDFGVSGQKNLKNLNVLIVGAGGLGCPVATYLGAAGIGTIGIVDY 69 Query: 60 QVPELSNLNRQILHWEEDLGRN-PKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 L NL+RQ+ + E+ +G++ + L+ KL+ NSD+ ++ L N + Sbjct: 70 DHISLDNLHRQVAYKEDQVGKSKAQALADNIKLQ--NSDLNVQVHNTSLDSSNAMQLFKN 127 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGK-TKRLREIFP--- 174 +++ DC DN TRYL++D + IPLV G+ GQ++ G R +FP Sbjct: 128 YEIVCDCTDNVATRYLINDVCVLLNIPLVSGSALRWDGQLSVYHYGSDCPCYRCLFPSPP 187 Query: 175 ---RIKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 + + +LG GVIGS+Q EV+K+ LA +LL+ D Sbjct: 188 DPNSVTNCNEGGVLGPIVGVIGSMQALEVMKIAAKVRTTLAGQLLLFD 235 >sp|Q09810|YABA_SCHPO HYPOTHETICAL 44.4 KD PROTEIN C2G11.10C IN CHROMOSOME I >gi|2130396|pir||S62465 probable molybdopterin synthase sulfurylase - fission yeast (Schizosaccharomyces pombe) >gi|1019408|emb|CAA91175.1| (Z54354) similar to yeast molybdopterin synthase sulfurylase [Schizosaccharomyces pombe] Length = 401 Score = 128 bits (319), Expect = 5e-29 Identities = 80/209 (38%), Positives = 114/209 (54%), Gaps = 7/209 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS E RY RQ+++ G+ GQ LK++ V V+G GGLG P YL AAG+G + ++D Sbjct: 16 LSLDEYSRYGRQMLLSEIGLPGQLSLKRSSVLVIGAGGLGCPAMQYLVAAGIGTLGIMDG 75 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 V + SNL+RQI+H G + K +SAK LE N +V I T++ + N+ ++ Sbjct: 76 DVVDKSNLHRQIIHSTSKQGMH-KAISAKQFLEDLNPNVIINTYLEFASASNLFSIIEQY 134 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPR---- 175 DV++DC DN TRYL+ D +G PLV + L GQ+ R +FP Sbjct: 135 DVVLDCTDNQYTRYLISDTCVLLGRPLVSASALKLEGQLCIYNYCNGPCYRCMFPNPTPV 194 Query: 176 IKKKGKFPILGATAGVIGSIQVTEVVKLI 204 + K ILG G +G++Q E VKLI Sbjct: 195 VASCAKSGILGPVVGTMGTMQALETVKLI 223 >gb|AAC35198.1| (AF003700) HesA [Cyanothece PCC8801] Length = 188 Score = 128 bits (319), Expect = 5e-29 Identities = 72/175 (41%), Positives = 107/175 (61%), Gaps = 3/175 (1%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L+ ELERY RQ+ + FG +GQ++LK + V GVGGLG VA YL AG+G+++L+ Sbjct: 3 LTPIELERYSRQMQLPGFGEKGQKQLKASTALVTGVGGLGGTVALYLAVAGIGKLILVRG 62 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 +LNRQIL ++ +G+ P+ AK L K N DV+IE +T EN+++++ Sbjct: 63 GELRRDDLNRQILMTDDWVGK-PRVFKAKETLSKINPDVEIEAVYDYVTPENVDELVQQA 121 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFP 174 D+ +DC +F R LL+ + G P+V A+ G+ +TTIIPG+T L IFP Sbjct: 122 DIALDCSFDFAERDLLNAACVRWGRPMVEAAMSGMDAYLTTIIPGETPCLSCIFP 176 >emb|CAB77681.1| (AL161416) molybdopterin synthase sulphurylase [Leishmania major] Length = 463 Score = 128 bits (319), Expect = 5e-29 Identities = 78/224 (34%), Positives = 123/224 (54%), Gaps = 9/224 (4%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L++ ++ER+ RQ+++ G ++++A V VG GGLGS + YL A+GVGR+ ++D Sbjct: 22 LTKEDVERFGRQMLVEEIGAAHMGQIRQAHVVCVGAGGLGSTILLYLAASGVGRLTIVDF 81 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 ELSNL+RQ++H +G+ PK LSAK + ++ V L+ N E + Sbjct: 82 DDVELSNLHRQVIHTTAAIGQ-PKALSAKASCLRLFPAACVDAVVEALSPANAERLFATC 140 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPR---- 175 DV+VD DN RYL++D A + G PLV G+ G GQ++ R +FP+ Sbjct: 141 DVVVDGTDNVAARYLINDAAMRCGKPLVSGSAMGWEGQLSVYGYRGGPCYRCLFPQPPPQ 200 Query: 176 --IKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLI 217 + ++G G+IG +Q EV+K+ TG GE L +L + Sbjct: 201 ETVGSCNDSGVMGPLPGMIGCLQALEVLKVATGVGETLNGRLFL 244 >pir||C49695 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate synthesis protein ThiF - Escherichia coli >gi|414234|gb|AAB95618.1| (M88701) thiF [Escherichia coli] Length = 250 Score = 128 bits (318), Expect = 7e-29 Identities = 74/225 (32%), Positives = 129/225 (56%), Gaps = 9/225 (4%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 +++ + RY RQI++ ++GQ+KL ++V ++G+GGLG+P A YL AGVG ++L D+ Sbjct: 1 MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAALYLAGAGVGTLVLADD 60 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 LSNL RQIL ED+ R PK ++ +L + N D+++ +LT E ++D + Sbjct: 61 DDVHLSNLQRQILFTTEDIDR-PKSQVSQQRLTQLNPDIQLTALQQRLTGEALKDAVARA 119 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTII-PGKTKRLREIFPRIKK 178 DV++DC DN TR ++ + PL+ + G GQ+ + P + R ++P ++ Sbjct: 120 DVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMVLTPPWEQGCYRCLWPDNQE 179 Query: 179 KGK----FPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 + ++G GV+G++Q E +KL++G P A +L + D Sbjct: 180 PERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETP-AGELRLFD 223 >sp|P30138|THIF_ECOLI THIF PROTEIN Length = 251 Score = 128 bits (318), Expect = 7e-29 Identities = 74/225 (32%), Positives = 129/225 (56%), Gaps = 9/225 (4%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 +++ + RY RQI++ ++GQ+KL ++V ++G+GGLG+P A YL AGVG ++L D+ Sbjct: 1 MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAALYLAGAGVGTLVLADD 60 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 LSNL RQIL ED+ R PK ++ +L + N D+++ +LT E ++D + Sbjct: 61 DDVHLSNLQRQILFTTEDIDR-PKSQVSQQRLTQLNPDIQLTALQQRLTGEALKDAVARA 119 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTII-PGKTKRLREIFPRIKK 178 DV++DC DN TR ++ + PL+ + G GQ+ + P + R ++P ++ Sbjct: 120 DVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMVLTPPWEQGCYRCLWPDNQE 179 Query: 179 KGK----FPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 + ++G GV+G++Q E +KL++G P A +L + D Sbjct: 180 PERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETP-AGELRLFD 223 >pir||C65206 thiF protein - Escherichia coli >gi|396331|gb|AAC43090.1| (U00006) similar to E. coli ChlN [Escherichia coli] >gi|1790425|gb|AAC76966.1| (AE000473) thiamin biosynthesis, thiazole moiety [Escherichia coli K12] Length = 245 Score = 126 bits (314), Expect = 2e-28 Identities = 74/218 (33%), Positives = 125/218 (56%), Gaps = 9/218 (4%) Query: 9 RYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSN 66 RY RQI++ ++GQ+KL ++V ++G+GGLG+P A YL AGVG ++L D+ LSN Sbjct: 2 RYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAALYLAGAGVGTLVLADDDDVHLSN 61 Query: 67 LNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCL 126 L RQIL ED+ R PK ++ +L + N D+++ +LT E ++D + DV++DC Sbjct: 62 LQRQILFTTEDIDR-PKSQVSQQRLTQLNPDIQLTALQQRLTGEALKDAVARADVVLDCT 120 Query: 127 DNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTII-PGKTKRLREIFPRIKKKGK---- 181 DN TR ++ + PL+ + G GQ+ + P + R ++P ++ + Sbjct: 121 DNMATRQEINAACVALNTPLITASAVGFGGQLMVLTPPWEQGCYRCLWPDNQEPERNCRT 180 Query: 182 FPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 ++G GV+G++Q E +KL++G P A +L + D Sbjct: 181 AGVVGPVVGVMGTLQALEAIKLLSGIETP-AGELRLFD 217 >gb|AAC83136.1| (AF109295) MoeB [Staphylococcus carnosus] Length = 333 Score = 126 bits (313), Expect = 3e-28 Identities = 74/226 (32%), Positives = 124/226 (54%), Gaps = 21/226 (9%) Query: 8 ERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELS 65 ERY RQI+ GVEGQEK+ + V ++G+G LG+ +A L AGV ++ ++D E S Sbjct: 3 ERYSRQILFKNIGVEGQEKISQKHVLIIGMGALGTHLADGLVRAGVRKLTIVDRDYIEFS 62 Query: 66 NLNRQILHWEEDLGRN-PKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVD 124 NL RQ ++ E D PK ++AK +L + SD++I+ ++ ++ +E+ VD+I+D Sbjct: 63 NLQRQTMYTENDAKEALPKVIAAKERLTEIRSDIEIDAYIDQVNPPFLEEHAQHVDLILD 122 Query: 125 CLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFPI 184 DNF+TR L++D+A+K IP ++G V IPG+T + P++ P+ Sbjct: 123 ATDNFDTRLLVNDFAYKYNIPWIYGGVVQSTYIEAAFIPGQTPCFNCVMPQL------PV 176 Query: 185 LGATAGVIG----------SIQVTEVVKLITGYGEPLANKLLIVDL 220 + T +G S+Q+ + +K++T P KL D+ Sbjct: 177 INMTCDTVGVIQPAVTMTTSLQLRDALKILT--DTPFTPKLTYGDI 220 >gb|AAF33509.1| (AF170176) 84% identity over 251 amino acids with E. coli THIF protein (SW:P30138); contains similarity to Pfam domain PF00899 (ThiF_family), Score=199.9, E=3.9e-56, N=1 [Salmonella typhimurium LT2] Length = 252 Score = 125 bits (312), Expect = 3e-28 Identities = 72/216 (33%), Positives = 122/216 (56%), Gaps = 8/216 (3%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 +++ + RY RQI++ +EGQ+KL + V +VG+GGLGSP A YL AG+G++ L+D+ Sbjct: 1 MNDHDFMRYSRQILLGDIAIEGQQKLLNSHVLIVGLGGLGSPAALYLAGAGIGKLTLVDD 60 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 LSNL RQIL +D+ +PK +AK +L + N K+ +LT + +++ + Sbjct: 61 DDVHLSNLQRQILFTTDDIA-HPKAQAAKLRLAQLNPGSKLIVLQQRLTGDVLKNAVAHA 119 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTII-PGKTKRLREIFPRIKK 178 DV++DC DN TR ++ + PL+ + G GQ+ + P + R ++P + Sbjct: 120 DVVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMVLTPPWEQGCYRCLWPDDVE 179 Query: 179 KGK----FPILGATAGVIGSIQVTEVVKLITGYGEP 210 + I+G GV+G++Q E +KL++G P Sbjct: 180 PERNCRTAGIVGPVVGVMGTLQALEAIKLLSGIETP 215 >gb|AAC24520.1| (AF055287) molybdenum cofactor biosynthetic protein [Aspergillus nidulans] Length = 560 Score = 123 bits (305), Expect = 2e-27 Identities = 85/224 (37%), Positives = 116/224 (50%), Gaps = 17/224 (7%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L E RY RQ+++ FG++GQ KL+ AKV +VG GGLG P A YL AGVG I L+D Sbjct: 139 LHGEEYRRYGRQMIVPQFGLQGQLKLRDAKVLIVGAGGLGCPAALYLAGAGVGTIGLVDG 198 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 E SNL+RQ+LH ++G+ K SA L + N LT D+ Sbjct: 199 DTVEASNLHRQVLHRSRNVGK-LKVDSAIEYLRELNPHPTYIAHQAHLTPREAPDIFKDY 257 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTI-----IPG-KT--KRLRE 171 D+I+DC DN TRYL+ D A +G PLV + GQ+ + PG KT R Sbjct: 258 DLILDCTDNPATRYLISDTAVLLGKPLVSASALRTEGQLMVLNNPPQPPGDKTGGPCYRC 317 Query: 172 IFPR------IKKKGKFPILGATAGVIGSIQVTEVVKLITGYGE 209 +FP+ + ILG G +G +Q +E +K++T GE Sbjct: 318 VFPKPPPANSVTSCADGGILGPVVGTMGVLQASEAIKVLTSAGE 361 >gb|AAD17271.1| (AF119361) unknown [Frankia sp. EuIK1] Length = 325 Score = 123 bits (305), Expect = 2e-27 Identities = 82/227 (36%), Positives = 126/227 (55%), Gaps = 10/227 (4%) Query: 9 RYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSN 66 RYDRQ+ + FG QE+L+ A V V G+GG+G A YL AAGVGR++L+ E ++ Sbjct: 75 RYDRQLGLPGFGPAAQERLRGATVLVAGIGGVGGAAATYLAAAGVGRLVLVHPGALEEAD 134 Query: 67 LNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTE-ENIEDVLHGVDVIVDC 125 LNRQ L E +G + A+ L + V+I +L++ + ++ DV+VD Sbjct: 135 LNRQTLMRPEWIG-GSRVACAEQTLRAHHPGVEIVALDRELSQLPELGRLVAEADVVVDA 193 Query: 126 LDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIF----PRIKKKGK 181 NF RYLL+D P V A+ G + + PG + LR +F P + G Sbjct: 194 RHNFPDRYLLNDVCVATRTPAVVAAMNATEGNMLVVRPG-SPCLRCVFAEGDPSWQPLG- 251 Query: 182 FPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEII 228 F +LGA +G++G + TE +K+I+G+GEP A +LL DL + F+++ Sbjct: 252 FTVLGAVSGMVGCLAATEAIKIISGFGEPAAGRLLQFDLWDLDFQVL 298 >gi|6321903 moeB, thiF, UBA1; Yhr111wp [Saccharomyces cerevisiae] >gi|731699|sp|P38820|YHR1_YEAST HYPOTHETICAL 49.4 KD PROTEIN IN CDC12-ORC6 INTERGENIC REGION >gi|630126|pir||S48953 hypothetical protein YHR111w - yeast (Saccharomyces cerevisiae) >gi|529119|gb|AAB68852.1| (U00059) Yhr111wp [Saccharomyces cerevisiae] Length = 440 Score = 122 bits (304), Expect = 3e-27 Identities = 78/215 (36%), Positives = 113/215 (52%), Gaps = 11/215 (5%) Query: 2 LSESELERYDRQIMIF---GVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 LS E +RY RQ+++ GV GQ KLK KV VVG GGLG P YL AGVG+I ++D Sbjct: 39 LSLEEYQRYGRQMIVEETGGVAGQVKLKNTKVLVVGAGGLGCPALPYLAGAGVGQIGIVD 98 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 V E SNL+RQ+LH +G K SA+ + K N + + T+ +L N D+ G Sbjct: 99 NDVVETSNLHRQVLHDSSRVGM-LKCESARQYITKLNPHINVVTYPVRLNSSNAFDIFKG 157 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIF----- 173 + I+DC D+ TRYL+ D A +GI +V + G GQ+T + F Sbjct: 158 YNYILDCTDSPLTRYLVSDVAVNLGITVVSASGLGTEGQLTILNFNNIGPCYRCFYPTPP 217 Query: 174 --PRIKKKGKFPILGATAGVIGSIQVTEVVKLITG 206 + + ++G G++G++ E +KLI G Sbjct: 218 PPNAVTSCQEGGVIGPCIGLVGTMMAVETLKLILG 252 >gi|11465494 unknown; protochlorophyllide reductase chlN chain [Cyanidium caldarium] >gi|6466397|gb|AAF12979.1|AF022186_102 (AF022186) unknown; protochlorophyllide reductase chlN chain [Cyanidium caldarium] Length = 395 Score = 122 bits (303), Expect = 4e-27 Identities = 74/238 (31%), Positives = 128/238 (53%), Gaps = 9/238 (3%) Query: 1 MLSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 +L++ + Y+RQ+++ G+ GQ +KK++V VG G LG+ YL AAG GR+ ++D Sbjct: 7 ILTDFDYRMYNRQMILPELGLNGQINIKKSRVLCVGAGALGASSLMYLCAAGFGRLGIVD 66 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 +SNL RQI+H E + K SA L++ N +KI+ + +L+ N ++ Sbjct: 67 FDRVAISNLQRQIIHTYEAI-NTYKTFSAFNVLKQLNIHIKIDLYTTRLSSLNAVKLISY 125 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK 178 D+++D DNFET+ +++D + P+++ AV GQ++ R I ++ Sbjct: 126 YDLVIDASDNFETKCIINDTCQLLKKPMIYAAVLKFIGQISVFNYRGGPSYRTICSKLPD 185 Query: 179 KGKFP------ILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 +F ++GA GV+GS+Q E VK+I G G+ L+ +L +D+ I I Sbjct: 186 SREFASCSESGVIGALPGVLGSLQAIEAVKVIIGKGQVLSGGILTLDVFKMFIRIFGI 243 >pir||B82369 thiF protein VC0063 [imported] - Vibrio cholerae (group O1 strain N16961) >gi|9654458|gb|AAF93241.1| (AE004097) thiF protein [Vibrio cholerae] Length = 258 Score = 119 bits (296), Expect = 3e-26 Identities = 76/233 (32%), Positives = 123/233 (52%), Gaps = 23/233 (9%) Query: 1 MLSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 ML++ + RY RQI + G EGQ+KL ++V +VG GGLG+ VA YL AGVG++++ D Sbjct: 1 MLTDKQFLRYQRQISLAELGEEGQQKLLNSRVLIVGCGGLGNVVAPYLVGAGVGQVIIAD 60 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 EL NL+RQI + E +G N L A++ L + NS+V++ ++ E + ++ Sbjct: 61 SDRLELHNLHRQICYHEAQIGHNKAELLARY-LRELNSEVRVRVIAREVDELILNLEINQ 119 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTT------------IIPGKT 166 VD+++DC DN TR+ ++ + PL+ GAV G G + ++P Sbjct: 120 VDLVLDCSDNLPTRHAINRACYAAQRPLISGAVIGWEGHLMAFDYRQSTPCYQCVVPDMA 179 Query: 167 KRLREIFPRIKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVD 219 +R R +G ++G G+IG+ Q + + G AN+LL D Sbjct: 180 ER-----QRCSDRG---VIGPVVGMIGNGQALIALHALMGSAHFPANQLLRFD 224 >pir||G69076 molybdopterin biosynthesis protein MoeB homolog - Methanobacterium thermoautotrophicum (strain Delta H) >gi|2622694|gb|AAB86045.1| (AE000917) molybdopterin biosynthesis protein MoeB homolog [Methanobacterium thermoautotrophicum] Length = 251 Score = 118 bits (292), Expect = 7e-26 Identities = 80/240 (33%), Positives = 122/240 (50%), Gaps = 18/240 (7%) Query: 8 ERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNL 67 E RQ+ + Q KLK + V+V+G GG+G L GVG + +ID V ++SN+ Sbjct: 12 EMVSRQMGLLSRADQLKLKDSTVSVIGCGGIGGAAVEMLARMGVGSLRIIDSDVFDVSNI 71 Query: 68 NRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCLD 127 NRQ++ DL R PK A ++ N +++ + EEN +++ G D +VD LD Sbjct: 72 NRQLMSSFRDL-RIPKVDVAAERIRTVNPFSRVKVYHEIFDEENAAEIIPGSDAVVDALD 130 Query: 128 NFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK-----RL----REIFPRIKK 178 N +R + GIP +HGA+ G GQVT G RL E+ +K Sbjct: 131 NITSRVIASRRCTSEGIPFIHGAIHGSMGQVTVFREGSPSYEELFRLPSVGLELTADVKA 190 Query: 179 KGKF------PILGATAGVIGSIQVTEVVKLITGYGEPL-ANKLLIVDL-ANNVFEIIEI 230 K + P++G A + G +Q EV+KLITG G+ + A ++L DL F+I+E+ Sbjct: 191 KLRDLSSKTPPVIGPVANITGCLQAAEVLKLITGRGDVIAAPRMLKFDLFQGEPFKIVEL 250 >gb|AAA87953.1| (U47055) HesA [Anabaena PCC7120] Length = 159 Score = 116 bits (287), Expect = 3e-25 Identities = 62/155 (40%), Positives = 95/155 (61%), Gaps = 3/155 (1%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 L+ +ELERY RQ+M+ FG Q++LK A V V GVGGLG A YL AGVGR++L+ Sbjct: 4 LTPTELERYSRQMMLPNFGEAAQKRLKSATVLVTGVGGLGGTAALYLAVAGVGRLILVRG 63 Query: 60 QVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGV 119 L ++NRQ+L ++ +G+ P+ AK L+ N D++IET +T EN++ ++ Sbjct: 64 GDLRLDDMNRQVLMTDDWVGK-PRVFKAKETLQAINPDIQIETIHDYITSENVDSLVQSA 122 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGL 154 D+ +DC NF R LL+ + P+V A++G+ Sbjct: 123 DMALDCAHNFTERDLLNSACVRWRKPMVEAAMDGM 157 >pir||H75294 molybdopterin biosynthesis MoeB - Deinococcus radiodurans (strain R1) >gi|6460075|gb|AAF11815.1|AE002059_5 (AE002059) molybdopterin biosynthesis MoeB [Deinococcus radiodurans] Length = 235 Score = 112 bits (278), Expect = 3e-24 Identities = 76/235 (32%), Positives = 118/235 (49%), Gaps = 10/235 (4%) Query: 2 LSESELERYDRQIMI---FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLID 58 L+ EL R R +++ QEKL+ A+V VVG GGLG PV L AGVG + + D Sbjct: 3 LTREELRRVSRPLLVPEWAEAGAQEKLRAARVLVVGAGGLGGPVIRQLAGAGVGALTVAD 62 Query: 59 EQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG 118 ++NL+RQ L+ D+GR+ K +A + N V+I+ LT E ++ Sbjct: 63 GDTVSVTNLHRQQLYRTADVGRS-KAETACAVAQGVNPFVQIQA-APALTPETAPTLIAA 120 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKK 178 D+ VD DNFETRYL+ D ++G V GA G+ G + G LR++FP + Sbjct: 121 HDLTVDATDNFETRYLIADTCRELGREWVWGAASGVSGLCSVF--GPHLGLRDVFPTPED 178 Query: 179 KG---KFPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFEIIEI 230 + +LG ++G + + + ++ G GEPL KL D ++++ Sbjct: 179 DSSCDELGVLGPVPNLVGDLMALQALLVLGGVGEPLRGKLWTFDALTGRVRVLKM 233 >emb|CAC11973.1| (AL445065) molybdopterin biosynthesis protein moeB related protein [Thermoplasma acidophilum] Length = 261 Score = 109 bits (271), Expect = 2e-23 Identities = 70/207 (33%), Positives = 115/207 (54%), Gaps = 13/207 (6%) Query: 9 RYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSN 66 +Y RQ+++ F + K+++A++ VVG+GG+GS +A L +GV +++LID SN Sbjct: 7 KYARQMVLRQFDKDDIAKIRRARILVVGLGGVGSLIADLLVRSGVKKVVLIDRDYVSSSN 66 Query: 67 LNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCL 126 L RQ L+ EED+G + K +AK +L+K N++V++E N E ++ VD+++D Sbjct: 67 LYRQTLYSEEDIG-DSKVEAAKRRLQKVNTEVEVEAINETFDATNAEALVSSVDLVMDGT 125 Query: 127 DNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIF-------PRIKKK 179 DN +R +++D K+ P V + YGQV +IP KT R F P + Sbjct: 126 DNLTSRLIINDACVKLKRPWVMASAIETYGQVKAVIPEKTSCYRCYFTSEPFAQPTCAEV 185 Query: 180 GKFPILGATAGVIGSIQVTEVVKLITG 206 G +L + I SI T +K++TG Sbjct: 186 G---VLNSLVTAISSIAFTLAMKILTG 209 >pir||B81010 thiF protein NMB2062 [imported] - Neisseria meningitidis (group B strain MD58) >gi|7227324|gb|AAF42381.1| (AE002556) thiF protein [Neisseria meningitidis MC58] Length = 256 Score = 108 bits (266), Expect = 8e-23 Identities = 69/221 (31%), Positives = 106/221 (47%), Gaps = 13/221 (5%) Query: 7 LERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPEL 64 L RY R I++ G+EGQ+KL A + VVG GGLG+ YL A+GVG + + D EL Sbjct: 13 LLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGTLTIADSDTVEL 72 Query: 65 SNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVD 124 NL RQ+ E D+G+ K + +L++ N V + KL + ++ D+++D Sbjct: 73 HNLQRQVAFDEGDVGK-LKTEALAGRLKRINHTVNVRAVNEKLDGCRLTGLVQAADIVLD 131 Query: 125 CLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGK--- 181 C DN+ TR ++ + PLV GA GQ+ P + + G Sbjct: 132 CCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPD--SPCYACLFDGGSASD 189 Query: 182 -----FPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLI 217 F + G+IGS Q E +K++ GEP +L + Sbjct: 190 GICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAV 230 >pir||C81953 ThiF protein NMA0373 [imported] - Neisseria meningitidis (group A strain Z2491) >gi|7379126|emb|CAB83674.1| (AL162753) ThiF protein [Neisseria meningitidis Z2491] Length = 256 Score = 107 bits (265), Expect = 1e-22 Identities = 69/221 (31%), Positives = 104/221 (46%), Gaps = 13/221 (5%) Query: 7 LERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPEL 64 L RY R I++ G+EGQ+KL A + VVG GGLG+ YL A+G+G + + D EL Sbjct: 13 LLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGTLTIADSDTVEL 72 Query: 65 SNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVD 124 NL RQ+ E D+G+ A +L N V + T KL + ++ D+++D Sbjct: 73 HNLQRQVAFDEGDVGKLKTEALAD-RLRHINHTVDVRTINEKLDGCRLTGLVQAADIVLD 131 Query: 125 CLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGK--- 181 C DN+ TR ++ + PLV GA GQ+ P + + G Sbjct: 132 CCDNYATRQAVNRACVQAKTPLVSGAAVRFEGQLAVYRPDLPD--SPCYACLFDGGSASD 189 Query: 182 -----FPILGATAGVIGSIQVTEVVKLITGYGEPLANKLLI 217 F + G+IGS Q E +K++ GEP +L + Sbjct: 190 GICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAV 230 >gb|AAK40637.1| Thiamine biosynthesis protein related protein (thiF) [Sulfolobus solfataricus] Length = 333 Score = 107 bits (265), Expect = 1e-22 Identities = 57/163 (34%), Positives = 93/163 (56%), Gaps = 1/163 (0%) Query: 7 LERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSN 66 +ERY RQ+++ G+ Q++L + K+ + G G LG+ VA L GV + ++D V +++N Sbjct: 43 VERYSRQLIVLGLGIQQRLNELKILIAGCGALGTAVAELLARLGVKELTIVDADVVDITN 102 Query: 67 LNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCL 126 L+R L E D+G+ + AK K+ NS +KI + L EEN+E ++ D + D L Sbjct: 103 LHRVHLFDENDVGKPKAEVCAK-KISLINSSIKINYIIDILDEENVERLISDKDYVFDAL 161 Query: 127 DNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRL 169 D+ + LL+D K+G L++G + G YG I P +T L Sbjct: 162 DSLYYKLLLNDAIVKLGKILIYGGINGEYGSAKLIDPSQTSCL 204 >gb|AAG31053.1|AF264948_15 (AF264948) thiamine biosynthesis protein ThiF [Erwinia amylovora] Length = 321 Score = 90.5 bits (221), Expect = 2e-17 Identities = 64/207 (30%), Positives = 96/207 (45%), Gaps = 8/207 (3%) Query: 7 LERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPEL 64 + RY RQ M+ G GQ++L +A+V V+G GGLGS + L AAG G + L D E Sbjct: 1 MSRYQRQSMLPEIGESGQQRLAQARVLVIGAGGLGSALLPLLAAAGAGYLRLYDGDRVEE 60 Query: 65 SNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVD 124 NL+RQ L+ +D+G K A+ L N ++ LT L G+D+ +D Sbjct: 61 HNLHRQTLYGMQDIGEE-KVFCARRALAIRNPACVVDARPHALTASATAAALDGIDLAID 119 Query: 125 CLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKK----G 180 DNF Y L D IPLV +V G G V G R +FP++ Sbjct: 120 AADNFALTYQLSDACLAHRIPLVSASVLGRRGYVGGFC-GGAPSYRALFPQLPGSAANCN 178 Query: 181 KFPILGATAGVIGSIQVTEVVKLITGY 207 ++G +G++Q + ++ + Sbjct: 179 TAGVMGPAVATLGAMQAQMALSILLNF 205 >gb|AAD00512.2| (U81376) PaaA [Erwinia herbicola] Length = 373 Score = 86.6 bits (211), Expect = 2e-16 Identities = 74/266 (27%), Positives = 123/266 (45%), Gaps = 47/266 (17%) Query: 1 MLSESELERYDRQIMIFGVEG---------QEKLKKAKVAVVGVGGLGSPVAYYLTAAGV 51 +LS E+ERY+RQI+ F + QE+LK++KVA+ G+GG G+ A L +G+ Sbjct: 97 VLSGEEVERYNRQILQFSLIDADNQHPFVYQERLKQSKVAIFGMGGWGTWCALQLAMSGI 156 Query: 52 GRILLIDEQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETF-------- 103 G + LID ELSN+NRQ+L+ +D+G+N K +AK + +N +V +ETF Sbjct: 157 GTLRLIDGDDVELSNINRQVLYRTDDVGKN-KVDAAKDTILAYNENVHVETFFEFASPDR 215 Query: 104 -------------------VGKLTEENIEDVLHGV---------DVIVDCLDNFETRYLL 135 +G ++ E+++H + ++ D L+ L Sbjct: 216 ARLEELVGDSTFIILAWAALGYYRKDTAEEIIHSIAKDKAIPVIELGGDPLEISVGPIYL 275 Query: 136 DDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFPILGATAGVIGSI 195 +D H + + + Y + I + RL+ F +K + ++ I Sbjct: 276 NDGVHSGFDEVKNSVKDKYYDSNSDIRKFQEARLKHSFIDGDRKVNAWQSAPSLSIMAGI 335 Query: 196 QVTEVVKLITGYGEP-LANKLLIVDL 220 +VVK ITGY +P L K I+ L Sbjct: 336 VTDQVVKTITGYDKPHLVGKKFILSL 361 >gb|AAB37569.1| (U40566) ubiquitin activating enzyme 2 [Arabidopsis thaliana] >gi|9758426|dbj|BAB08968.1| (AB006700) ubiquitin activating enzyme 2 [Arabidopsis thaliana] Length = 1077 Score = 86.2 bits (210), Expect = 3e-16 Identities = 55/157 (35%), Positives = 88/157 (56%), Gaps = 7/157 (4%) Query: 4 ESELERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPE 63 E + + + RQ+ ++G E KL + V + G+ GLG +A + AGV + L DE V E Sbjct: 69 EIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGVEIAKNIILAGVKSVTLHDENVVE 128 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIV 123 L +L+ + EED+G+N + L++ KL++ N+ V + T GKLT+E + D V V V Sbjct: 129 LWDLSSNFVFTEEDIGKN-RALASVHKLQELNNAVAVSTLTGKLTKEQLSD--FQVVVFV 185 Query: 124 DCLDNFETRYLLDDY--AHKVGIPLVHGAVEGLYGQV 158 D +FE +DDY +H+ I + V GL+G + Sbjct: 186 DI--SFEKATEIDDYCHSHQPPIAFIKADVRGLFGSL 220 Score = 69.5 bits (167), Expect = 3e-11 Identities = 55/168 (32%), Positives = 78/168 (45%), Gaps = 10/168 (5%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV-----GRILLIDEQVPE 63 RYD QI +FG Q+KL+ A+V VVG G LG L GV G++ + D+ V E Sbjct: 471 RYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIE 530 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETF---VGKLTEENIEDVL-HGV 119 SNL+RQ L + ++G+ K A NS + I+ VG TE +D + Sbjct: 531 KSNLSRQFLFRDWNIGQ-AKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENL 589 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 V+V+ LDN R +D PL+ G +IP T+ Sbjct: 590 TVVVNALDNVTARLYVDSRCVYFQKPLLESGTLGAKCNTQMVIPHLTE 637 >pir||T39623 ubiquitin-activating enzyme e1-like - fission yeast (Schizosaccharomyces pombe) >gi|2956755|emb|CAA17901.1| (AL022104) ubiquitin-activating enzyme e1-like [Schizosaccharomyces pombe] Length = 628 Score = 83.1 bits (202), Expect = 3e-15 Identities = 49/150 (32%), Positives = 75/150 (49%), Gaps = 2/150 (1%) Query: 19 VEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDL 78 VE K AKV +VG GG+G + L +GV + +ID +LSNLNRQ L ++ + Sbjct: 17 VEALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHV 76 Query: 79 GRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDV-LHGVDVIVDCLDNFETRYLLDD 137 + PK + A FN +VK+E + + E+ D++ + LDN + R ++ Sbjct: 77 -KQPKAIVAAKTASSFNPNVKLEAYHANIKEDRFNVAWFRQFDLVFNALDNLDARRHVNK 135 Query: 138 YAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 +PL+ G GQV II GKT+ Sbjct: 136 QCLLASVPLIESGTTGFLGQVQVIIHGKTE 165 >dbj|BAB15587.1| (AK026904) unnamed protein product [Homo sapiens] Length = 404 Score = 80.8 bits (196), Expect = 1e-14 Identities = 62/216 (28%), Positives = 102/216 (46%), Gaps = 29/216 (13%) Query: 19 VEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDL 78 V EK++ VA+VGVGG+GS A LT G+G++LL D EL+N+NR L ++ Sbjct: 65 VSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNR--LFFQPHQ 122 Query: 79 GRNPKPLSAKWKLEKFNSDVKIET-------------FVGKLTEENIEDVLHGVDVIVDC 125 K +A+ L N DV E F+ +++ +E+ VD+++ C Sbjct: 123 AGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEG-KPVDLVLSC 181 Query: 126 LDNFETRYLLDDYAHKVGIPLVHGAV--EGLYGQVTTIIPGKTKRLREIFP--------- 174 +DNFE R ++ +++G + V + G + IIPG++ P Sbjct: 182 VDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDE 241 Query: 175 -RIKKKGKFPI-LGATAGVIGSIQVTEVVKLITGYG 208 +K++G L T GV+ I V V+K + +G Sbjct: 242 KTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFG 277 >pir||T38013 hypothetical protein SPAC1A6.10 - fission yeast (Schizosaccharomyces pombe) >gi|2414593|emb|CAB16360.1| (Z99258) hypothetical protein [Schizosaccharomyces pombe] Length = 485 Score = 80.8 bits (196), Expect = 1e-14 Identities = 58/220 (26%), Positives = 106/220 (47%), Gaps = 12/220 (5%) Query: 8 ERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNL 67 E+ R FG +G E+L+ + V VVG GG+GS V L +GV +I ++D LS+L Sbjct: 107 EQLARNYAFFGEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQVSLSSL 166 Query: 68 NRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG-VDVIVDCL 126 NR + +D+G PK L+ K ++KF ++++ ++ +D+L G D ++D + Sbjct: 167 NRHSIATLQDVG-TPKTLAIKKAIKKFAPWIEVDARNALFNPDSADDLLSGNPDFVIDAI 225 Query: 127 DNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFPILG 186 DN +T+ L Y + +P++ +T K+ R I + P+ Sbjct: 226 DNIQTKVDLLSYCYNHKLPVI----------ASTGSACKSDPTRVNIADISATSEDPLSR 275 Query: 187 ATAGVIGSIQVTEVVKLITGYGEPLANKLLIVDLANNVFE 226 AT + + + E + ++ +P K ++ L+ FE Sbjct: 276 ATRRRLRLLGIMEGIPVVFSTEKPDPRKASLLPLSEEEFE 315 >emb|CAA75816.1| (Y15895) ubiquitin activating enzyme [Drosophila melanogaster] Length = 1008 Score = 80.8 bits (196), Expect = 1e-14 Identities = 57/167 (34%), Positives = 84/167 (50%), Gaps = 9/167 (5%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV----GRILLIDEQVPEL 64 RYD QI IFG + QEKL +K +VG G +G + G+ G+I + D + E Sbjct: 403 RYDSQIAIFGKKFQEKLADSKWFIVGAGAIGCELLKNFGMLGLGTGNGQIFVTDMDLIEK 462 Query: 65 SNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETF---VGKLTEENI-EDVLHGVD 120 SNLNRQ L D+ + PK ++A +++ N +V + + VG TE+ ED +D Sbjct: 463 SNLNRQFLFRPHDV-QKPKSMTAADAIKRMNPEVNVTAYELRVGAETEKVFSEDFFGKLD 521 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 + + LDN + R +D IPLV G G V I+P T+ Sbjct: 522 GVANALDNVDARIYMDRKCIFNRIPLVETGTLGTLGNVQVIVPFATE 568 >gb|AAF58910.1| (AE003832) Uba1 gene product [Drosophila melanogaster] Length = 1008 Score = 80.8 bits (196), Expect = 1e-14 Identities = 57/167 (34%), Positives = 84/167 (50%), Gaps = 9/167 (5%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV----GRILLIDEQVPEL 64 RYD QI IFG + QEKL +K +VG G +G + G+ G+I + D + E Sbjct: 403 RYDSQIAIFGKKFQEKLADSKWFIVGAGAIGCELLKNFGMLGLGTGNGQIFVTDMDLIEK 462 Query: 65 SNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETF---VGKLTEENI-EDVLHGVD 120 SNLNRQ L D+ + PK ++A +++ N +V + + VG TE+ ED +D Sbjct: 463 SNLNRQFLFRPHDV-QKPKSMTAADAIKRMNPEVNVTAYELRVGAETEKVFSEDFFGKLD 521 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 + + LDN + R +D IPLV G G V I+P T+ Sbjct: 522 GVANALDNVDARIYMDRKCIFNRIPLVETGTLGTLGNVQVIVPFATE 568 >gb|AAG23434.1|AC078892_6 (AC078892) Ubiquitin activating enzyme [Leishmania major] Length = 673 Score = 80.0 bits (194), Expect = 2e-14 Identities = 53/167 (31%), Positives = 83/167 (48%), Gaps = 10/167 (5%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV-----GRILLIDEQVPE 63 RYD I IFG Q+ L+ ++ +VG G LG G+ G +++ D E Sbjct: 468 RYDHIISIFGKGFQQLLQNLRLFMVGCGALGCENVKNFALCGITCGTGGSLVVTDNDRIE 527 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIET---FVGKLTEENIEDVL-HGV 119 +SNL+RQ L EE++G++ K +A ++ + N D ++ F+G TE D + Sbjct: 528 VSNLSRQFLFREENVGQS-KSAAATARMRQMNPDANVDARQDFIGTTTEHLYPDTFWQSL 586 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKT 166 +V+V+ LDN E R +D + LV G G V I+PG+T Sbjct: 587 NVVVNALDNIEARLYVDQQCVRFQKVLVEAGTMGTGGNVDIIVPGRT 633 >pir||S48571 ubiquitin--protein ligase E1-like protein - yeast (Saccharomyces cerevisiae) >gi|475916|emb|CAA82980.1| (Z30326) ubiquitin activating enzyme E1-like protein [Saccharomyces cerevisiae] Length = 294 Score = 79.2 bits (192), Expect = 4e-14 Identities = 48/166 (28%), Positives = 86/166 (50%), Gaps = 4/166 (2%) Query: 4 ESELERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPE 63 E ++ R + I G + +KL+ ++ +VG GG+GS + + G I ++D + Sbjct: 31 EKKMPRETSLVTIIGEDSYKKLRSSRCLLVGAGGIGSELLKDIILMEFGEIHIVDLDTID 90 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDV--LHGVDV 121 LSNLNRQ L ++D+ + PK +A ++ FN+ K+ + G L + + + D+ Sbjct: 91 LSNLNRQFLFRQKDI-KQPKSTTAVKAVQHFNNS-KLVPYQGNLMDISTFPLHWFEQFDI 148 Query: 122 IVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 I + LDN R ++ + + +PL+ G G + IIPGKT+ Sbjct: 149 IFNALDNLAARRYVNKISKFLSLPLIESGTAGFDGYMQPIIPGKTE 194 >gi|4507763 ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing); Ubiquitin-activating enzyme E1 (teA1S9) [Homo sapiens] >gi|267189|sp|P22314|UBA1_HUMAN UBIQUITIN-ACTIVATING ENZYME E1 (A1S9 PROTEIN) >gi|478678|pir||S23770 ubiquitin-activating enzyme E1 - human >gi|35830|emb|CAA40296.1| (X56976) ubiquitin activating enzyme E1 [Homo sapiens] Length = 1058 Score = 78.8 bits (191), Expect = 5e-14 Identities = 49/158 (31%), Positives = 83/158 (52%), Gaps = 7/158 (4%) Query: 3 SESELER--YDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ 60 SE++++ Y RQ+ + G E ++L+ + V V G+ GLG +A + GV + L D+ Sbjct: 46 SEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQG 105 Query: 61 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD 120 + ++L+ Q EED+G+N +S +L + NS V + + G L +ED L G Sbjct: 106 TAQWADLSSQFYLREEDIGKNRAEVSQP-RLAELNSYVPVTAYTGPL----VEDFLSGFQ 160 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQV 158 V+V E + + ++ H GI LV GL+GQ+ Sbjct: 161 VVVLTNTPLEDQLRVGEFCHNRGIKLVVAGTRGLFGQL 198 >gb|AAB71466.1| (AC000098) Similar to Caenorhabditis unknown protein T03F1.1 (gb|U88169). [Arabidopsis thaliana] Length = 445 Score = 78.8 bits (191), Expect = 5e-14 Identities = 50/166 (30%), Positives = 87/166 (52%), Gaps = 20/166 (12%) Query: 19 VEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDL 78 V+ E++++ VA+VG+GG+GS A LT G+GR+LL D EL+N+NR L + D Sbjct: 74 VDNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDTVELANMNR--LFFRPDQ 131 Query: 79 GRNPKPLSAKWKLEKFNSDVKI-------------ETFVGKLTEENI---EDVLHGVDVI 122 K +A L + N DV + ETF LT ++ ++ GVD++ Sbjct: 132 VGMTKTDAAVQTLAEINPDVVLESFTMNITTVQGFETFTSSLTNKSFCPSKEGGSGVDLV 191 Query: 123 VDCLDNFETRYLLDDYAHKVGIPLVHGAV--EGLYGQVTTIIPGKT 166 + C+DN+E R ++ +++ + V + + G + ++PG+T Sbjct: 192 LSCVDNYEARMAVNQACNELRQTWMESGVSEDAVSGHIQLLVPGET 237 >dbj|BAB10670.1| (AB025630) gene_id:MPA22.7~pir||T38013~similar to unknown protein [Arabidopsis thaliana] Length = 414 Score = 78.8 bits (191), Expect = 5e-14 Identities = 51/155 (32%), Positives = 77/155 (48%), Gaps = 2/155 (1%) Query: 8 ERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNL 67 E R I FG+E Q K+ + V V+G+GG+GS A L +GVG++LL+D LS+L Sbjct: 31 EHLTRNIQFFGLESQHKVTGSYVVVIGLGGVGSHAASMLLRSGVGKLLLVDFDQVSLSSL 90 Query: 68 NRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHG-VDVIVDCL 126 NR + D+G PK + K + IE V + E++L G D ++DC+ Sbjct: 91 NRHAVATRADVG-IPKAMCLKKHFSSIFPECHIEAKVMLYDSSSEEEILSGKPDFVLDCI 149 Query: 127 DNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTI 161 DN +T+ L K G+ ++ G T I Sbjct: 150 DNIDTKVGLLAACVKRGLKVLSATGAGARADPTRI 184 >sp|Q29504|UBA1_RABIT UBIQUITIN-ACTIVATING ENZYME E1 >gi|1381183|gb|AAC48768.1| (U58653) ubiquitin-activating enzyme E1 [Oryctolagus cuniculus] Length = 1058 Score = 78.4 bits (190), Expect = 7e-14 Identities = 49/158 (31%), Positives = 83/158 (52%), Gaps = 7/158 (4%) Query: 3 SESELER--YDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ 60 SE++++ Y RQ+ + G E ++L+ + V V G+ GLG +A + GV + L D+ Sbjct: 46 SEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQG 105 Query: 61 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD 120 + ++L+ Q EED+G+N +S +L + NS V + + G L +ED L G Sbjct: 106 TAQWADLSSQFYLREEDIGKNRAEVSQP-RLAELNSYVPVTAYTGPL----VEDFLSGFQ 160 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQV 158 V+V E + + ++ H GI LV GL+GQ+ Sbjct: 161 VVVLTNSPLEDQLRVGEFCHSRGIKLVVADTRGLFGQL 198 >pir||A38564 ubiquitin--protein ligase (EC 6.3.2.19) E1 - human >gi|340072|gb|AAA61246.1| (M58028) ubiquitin-activating enzyme E1 [Homo sapiens] Length = 1058 Score = 78.0 bits (189), Expect = 9e-14 Identities = 49/158 (31%), Positives = 83/158 (52%), Gaps = 7/158 (4%) Query: 3 SESELER--YDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ 60 SE++++ Y RQ+ + G E ++L+ + V V G+ GLG +A + GV + L D+ Sbjct: 46 SEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQG 105 Query: 61 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD 120 + ++L+ Q EED+G+N +S +L + NS V + + G L +ED L G Sbjct: 106 TAQWADLSSQFYLREEDIGKNRAEVSQP-RLAELNSYVPVTAYTGPL----VEDFLSGFQ 160 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQV 158 V+V E + + ++ H GI LV GL+GQ+ Sbjct: 161 VVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQL 198 >gi|6320598 similar to ubiquitin activating enzyme (E1); Uba2p [Saccharomyces cerevisiae] >gi|1717852|sp|P52488|UBA2_YEAST UBIQUITIN-ACTIVATING ENZYME E1-LIKE (POLYMERASE-INTERACTING PROTEIN 2) >gi|1362482|pir||A57178 ubiquitin-activating enzyme homolog UBA2 - yeast (Saccharomyces cerevisiae) >gi|793879|emb|CAA88617.1| (Z48725) Uba2 protein [Saccharomyces cerevisiae] >gi|927323|gb|AAB64832.1| (U32274) Uba2p; CAI: 0.15 [Saccharomyces cerevisiae] >gi|1835684|gb|AAB46626.1| (U17263) Pip2p [Saccharomyces cerevisiae] Length = 636 Score = 76.1 bits (184), Expect = 3e-13 Identities = 45/156 (28%), Positives = 82/156 (51%), Gaps = 4/156 (2%) Query: 14 IMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILH 73 + I G + +KL+ ++ +VG GG+GS + + G I ++D +LSNLNRQ L Sbjct: 8 VTIIGEDSYKKLRSSRCLLVGAGGIGSELLKDIILMEFGEIHIVDLDTIDLSNLNRQFLF 67 Query: 74 WEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDV--LHGVDVIVDCLDNFET 131 ++D+ + PK +A ++ FN+ K+ + G + + + + D+I + LDN Sbjct: 68 RQKDI-KQPKSTTAVKAVQHFNNS-KLVPYQGNVMDISTFPLHWFEQFDIIFNALDNLAA 125 Query: 132 RYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 R ++ + + +PL+ G G + IIPGKT+ Sbjct: 126 RRYVNKISQFLSLPLIESGTAGFDGYMQPIIPGKTE 161 >pir||T29201 hypothetical protein T03F1.1 - Caenorhabditis elegans >gi|1825606|gb|AAB42231.1| (U88169) similar to molybdoterin biosynthesis MOEB proteins [Caenorhabditis elegans] Length = 419 Score = 76.1 bits (184), Expect = 3e-13 Identities = 60/217 (27%), Positives = 101/217 (45%), Gaps = 30/217 (13%) Query: 19 VEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDL 78 V E++++ VAVVGVGG+GS VA LT G+G+++L D E++N+NR L ++ + Sbjct: 76 VNEYERIREKTVAVVGVGGVGSVVAEMLTRCGIGKLILFDYDKVEIANMNR--LFYQPNQ 133 Query: 79 GRNPKPLSAKWKLEKFNSDVKIE-------------TFVGKLTEENIEDVLHGVDVIVDC 125 K +A+ L N DV+IE TFV ++ + ++ D +D+++ C Sbjct: 134 AGLSKVEAARDTLIHVNPDVQIEVHNFNITTMDNFDTFVNRIRKGSLTD--GKIDLVLSC 191 Query: 126 LDNFETRYLLDDYAHKVGIPLVHGAV--EGLYGQVTTIIPGKTKRLREIFPRIKKKG--- 180 +DNFE R ++ ++ + V + G + I PGKT + P + G Sbjct: 192 VDNFEARMAVNMACNEENQIWMESGVSENAVSGHIQYIEPGKTACFACVPPLVVASGIDE 251 Query: 181 --------KFPILGATAGVIGSIQVTEVVKLITGYGE 209 L T V+ V +K + +GE Sbjct: 252 RTLKRDGVCAASLPTTMAVVAGFLVMNTLKYLLNFGE 288 >emb|CAA40809.1| (X57583) mccB [Escherichia coli] Length = 350 Score = 75.7 bits (183), Expect = 5e-13 Identities = 62/235 (26%), Positives = 104/235 (43%), Gaps = 26/235 (11%) Query: 3 SESELERYDRQIMIFGVEG------QEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILL 56 + +E RY R + + G Q+KLK AKV ++G GG+G+ V+ L +G+G I+L Sbjct: 85 NSTENNRYSRNFLHYQSYGANPVLVQDKLKNAKVVILGCGGIGNHVSVILATSGIGEIIL 144 Query: 57 IDEQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVL 116 ID E +NL RQ+L E+D+G+N K K +L K NS++ + + + + Sbjct: 145 IDNDQIENTNLTRQVLFSEDDVGKN-KTEVIKRELLKRNSEISVSEIALNINDYTDLHKV 203 Query: 117 HGVDVIVDCLDN-FETRYLLDDYAHKVGIPLVH-GAVEGLYGQVTTIIPGKT-------- 166 D+ V D+ F ++ Y + P ++ G V + +PGKT Sbjct: 204 PEADIWVVSADHPFNLINWVNKYCVRANQPYINAGYVNDIAVFGPLYVPGKTGCYECQKV 263 Query: 167 ---------KRLREIFPRIKKKGKFPILGATAGVIGSIQVTEVVKLITGYGEPLA 212 + + I + K V ++ +V+K I Y EPL+ Sbjct: 264 VADLYGSEKENIDHKIKLINSRFKPATFAPVNNVAAALCAADVIKFIGKYSEPLS 318 >gi|6678483 ubiquitin-activating enzyme E1, Chr X [Mus musculus] >gi|267190|sp|Q02053|UBA1_MOUSE UBIQUITIN-ACTIVATING ENZYME E1 1 >gi|111228|pir||JC1254 ubiquitin--protein ligase (EC 6.3.2.19) E1 - mouse >gi|220629|dbj|BAA01433.1| (D10576) ubiquitin activating enzyme E1 [Mus musculus] Length = 1058 Score = 75.3 bits (182), Expect = 6e-13 Identities = 48/158 (30%), Positives = 81/158 (50%), Gaps = 7/158 (4%) Query: 3 SESELER--YDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQ 60 SE++++ Y RQ+ + G E + L+ + V V G+ GLG +A + GV + L D+ Sbjct: 46 SEADIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQG 105 Query: 61 VPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD 120 + ++L+ Q EED+G+N +S +L + NS V + + G L +ED L Sbjct: 106 TTQWADLSSQFYLREEDIGKNRAEVSQP-RLAELNSYVPVTAYTGPL----VEDFLSSFQ 160 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQV 158 V+V E + + ++ H GI LV GL+GQ+ Sbjct: 161 VVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQL 198 Score = 68.3 bits (164), Expect = 8e-11 Identities = 48/167 (28%), Positives = 75/167 (44%), Gaps = 8/167 (4%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV-----GRILLIDEQVPE 63 RYD Q+ +FG + QEKL K K +VG G +G + G+ G +++ D E Sbjct: 450 RYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMDTIE 509 Query: 64 LSNLNRQILH--WEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENI-EDVLHGVD 120 SNLNRQ L W+ ++ +A ++ + + VG TE +D +D Sbjct: 510 KSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDFFQNLD 569 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 + + LDN + R +D PL+ G G V +IP T+ Sbjct: 570 GVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTE 616 >dbj|BAA94076.1| (AB035495) ubiquitin-activating enzyme E1 [Carassius auratus] Length = 1058 Score = 75.3 bits (182), Expect = 6e-13 Identities = 46/149 (30%), Positives = 77/149 (50%), Gaps = 5/149 (3%) Query: 10 YDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNR 69 Y RQ+ + G E ++++ + V + G+ GLG +A + GV + L D+ V E +L+ Sbjct: 55 YSRQLYVLGHEAMKRMQSSSVLISGLRGLGVEIAKNVILGGVKSVTLHDQGVAEWKDLSS 114 Query: 70 QILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCLDNF 129 Q EEDLG+N +S + +L + NS V + ++ G L E L V+V + Sbjct: 115 QFYLREEDLGKNRAEVS-QTRLAELNSYVPVTSYTGALNNE----FLTKFQVVVLTNSSL 169 Query: 130 ETRYLLDDYAHKVGIPLVHGAVEGLYGQV 158 + + L D+ H GI L+ GL+GQ+ Sbjct: 170 DEQIRLGDFCHSNGIKLIVADTRGLFGQL 198 Score = 67.9 bits (163), Expect = 1e-10 Identities = 53/167 (31%), Positives = 80/167 (47%), Gaps = 9/167 (5%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPV----AYYLTAAGVGRILLIDEQVPEL 64 RYD QI +FG + QE L K + +VG G +G + A A+G G +++ D E Sbjct: 451 RYDGQIAVFGSKLQELLAKQRYFLVGAGAIGCELLKNFAMMGLASGEGEVIVTDMDTIEK 510 Query: 65 SNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKI---ETFVGKLTEENI-EDVLHGVD 120 SNLNRQ L D+ + K +A ++ N V+I + VG TE+ +D +D Sbjct: 511 SNLNRQFLFRPWDVTK-MKSETAAAAVKLMNPSVRITGHQNRVGPETEKVYDDDFFESLD 569 Query: 121 VIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 + + LDN + R +D PL+ G G V +IP T+ Sbjct: 570 GVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTE 616 >gb|AAF25197.1|AF193553_1 (AF193553) ubiquitin-like protein activating enzyme [Drosophila melanogaster] Length = 700 Score = 74.9 bits (181), Expect = 8e-13 Identities = 55/163 (33%), Positives = 80/163 (48%), Gaps = 4/163 (2%) Query: 22 QEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDLGRN 81 QE +KK+KV VVG GG+G V L +G I +ID +LSNLNRQ L E +G++ Sbjct: 14 QELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIIDLDTIDLSNLNRQFLFHREHVGKS 73 Query: 82 PKPLSAKWKLEKFNSDVKIETFVGKLTEENI-EDVLHGVDVIVDCLDNFETRYLLDDYAH 140 K A+ FN D KI + +T + + D+++ LDN R ++ Sbjct: 74 -KARVARESALSFNPDAKITAYHDSVTSTDYGVNFFKKFDLVLSALDNRAARNHVNRMCL 132 Query: 141 KVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFP 183 +PL+ G GQV I G T + E P+ K++ FP Sbjct: 133 NADVPLIESGTAGYNGQVELIKRGLT-QCYECTPKDKQR-SFP 173 >gb|AAF50484.2| (AE003556) Uba2 gene product [Drosophila melanogaster] Length = 700 Score = 74.9 bits (181), Expect = 8e-13 Identities = 55/163 (33%), Positives = 80/163 (48%), Gaps = 4/163 (2%) Query: 22 QEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDLGRN 81 QE +KK+KV VVG GG+G V L +G I +ID +LSNLNRQ L E +G++ Sbjct: 14 QELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIIDLDTIDLSNLNRQFLFHREHVGKS 73 Query: 82 PKPLSAKWKLEKFNSDVKIETFVGKLTEENI-EDVLHGVDVIVDCLDNFETRYLLDDYAH 140 K A+ FN D KI + +T + + D+++ LDN R ++ Sbjct: 74 -KARVARESALSFNPDAKITAYHDSVTSTDYGVNFFKKFDLVLSALDNRAARNHVNRMCL 132 Query: 141 KVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFP 183 +PL+ G GQV I G T + E P+ K++ FP Sbjct: 133 NADVPLIESGTAGYNGQVELIKRGLT-QCYECTPKDKQR-SFP 173 >pir||C81307 probable molybdopterin biosynthesis protein Cj1046c [imported] - Campylobacter jejuni (strain NCTC 11168) >gi|6968482|emb|CAB73302.1| (AL139077) putative molybdopterin biosynthesis protein [Campylobacter jejuni] Length = 267 Score = 74.5 bits (180), Expect = 1e-12 Identities = 51/154 (33%), Positives = 85/154 (55%), Gaps = 9/154 (5%) Query: 23 EKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDLGRNP 82 +KLK +VAV G+GGLGS +A L +GVG + LID V E SNLNRQ + DLG+ Sbjct: 80 DKLKNGRVAVCGLGGLGSHIAINLARSGVGYLKLIDFDVIEPSNLNRQ-AYRVSDLGKF- 137 Query: 83 KPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCLDNFETRYLLDDYAH-- 140 K + K ++ + N + +E K+ E+N+E + +D++ + D+ + ++ H Sbjct: 138 KTEALKEQISEINPYISVEICTLKIDEDNLESLFKDIDIVCEAFDSAIAKAMIAQNFHRF 197 Query: 141 -KVGIPLVHGAVEGLYGQVTTIIPGKTKRLREIF 173 K GI + + G YG +I +T+++ + F Sbjct: 198 YKDGILICASGLAG-YGDSNSI---QTRKIAKNF 227 >pir||I49380 ubiquitin-activating enzyme - mouse (fragment) >gi|558992|gb|AAA81009.1| (U15990) ubiquitin-activating enzyme [Mus musculus] Length = 179 Score = 74.1 bits (179), Expect = 1e-12 Identities = 46/149 (30%), Positives = 77/149 (50%), Gaps = 5/149 (3%) Query: 10 YDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNR 69 Y RQ+ + G E + L+ + V + G+ GLG +A + GV + L D+ + + ++L+ Sbjct: 14 YSRQLYVLGHEAMKHLQASSVLISGLQGLGVEIAKNIILGGVKAVTLHDQGIAQWADLSS 73 Query: 70 QILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCLDNF 129 Q+ EED+G+N +S +L + NS V + + G L IE+ L G V+V Sbjct: 74 QVCLREEDIGKNRAEISQP-RLAELNSYVPVFAYTGPL----IEEFLSGFQVVVLTNTPL 128 Query: 130 ETRYLLDDYAHKVGIPLVHGAVEGLYGQV 158 E + + ++ H GI LV GL GQ+ Sbjct: 129 EYQLQVGEFCHSHGIKLVVADTRGLVGQL 157 >gb|AAD23691.1|AC006841_24 (AC006841) putative ubiquitin activating enzyme [Arabidopsis thaliana] Length = 700 Score = 74.1 bits (179), Expect = 1e-12 Identities = 47/147 (31%), Positives = 74/147 (49%), Gaps = 2/147 (1%) Query: 22 QEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDLGRN 81 Q +K AKV +VG GG+G + L +G I +ID E+SNLNRQ L +G++ Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQS 66 Query: 82 PKPLSAKWKLEKFNSDVKIETFVGKLTEENIE-DVLHGVDVIVDCLDNFETRYLLDDYAH 140 K A+ + +F ++ I ++ + + D DV+++ LDN + R ++ Sbjct: 67 -KAKVARDAVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCL 125 Query: 141 KVGIPLVHGAVEGLYGQVTTIIPGKTK 167 +PLV G GQVT I GKT+ Sbjct: 126 AADVPLVESGTTGFLGQVTVHIKGKTE 152 >gb|AAF48050.1| (AE003486) CG1749 gene product [Drosophila melanogaster] Length = 404 Score = 74.1 bits (179), Expect = 1e-12 Identities = 62/216 (28%), Positives = 97/216 (44%), Gaps = 27/216 (12%) Query: 19 VEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDL 78 V+ E+++ VA+VGVGG+GS A LT G+G+++L D EL+N+NR L + D Sbjct: 65 VKDYERIRYKAVAIVGVGGVGSVTADMLTRCGIGKLILFDYDKVELANMNR--LFFTPDQ 122 Query: 79 GRNPKPLSAKWKLEKFNSDVKIETFVGKLTE-ENIEDVL-----------HGVDVIVDCL 126 K +A L N DV+IET +T EN + L VD+++ C+ Sbjct: 123 AGLSKVAAAAATLSFINPDVEIETHNYNITTVENFDRFLDTISQGGRIAGQPVDLVLSCV 182 Query: 127 DNFETRYLLDDYAHKVGIPLVHGAV--EGLYGQVTTIIPGKTKRLREIFP---------- 174 DNFE R ++ ++ + V + G + I PG T P Sbjct: 183 DNFEARMAINAACNERNLNWFESGVSENAVSGHIQFIRPGDTACFACAPPLVVAENIDEK 242 Query: 175 RIKKKGKFPI-LGATAGVIGSIQVTEVVKLITGYGE 209 +K++G L T G+ V +K + +GE Sbjct: 243 TLKREGVCAASLPTTMGITAGFLVQNALKYLLNFGE 278 >dbj|BAB04974.1| (AP001511) unknown conserved protein [Bacillus halodurans] Length = 259 Score = 74.1 bits (179), Expect = 1e-12 Identities = 47/145 (32%), Positives = 77/145 (52%), Gaps = 2/145 (1%) Query: 4 ESELERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPE 63 E L ++ R + G +G E+LK++KVAV+GVGG+GS L +GVGRI+++D+ + Sbjct: 5 EGMLHQFSRNELAIGHDGLERLKQSKVAVLGVGGVGSFSVEALARSGVGRIVIVDKDDVD 64 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVL-HGVDVI 122 ++N+NRQI +GR PK K ++ N + ++ TEE E + D + Sbjct: 65 ITNVNRQIHALLSTVGR-PKVDLMKERISDINPECEVIALKMFYTEETYEQFFSYEPDYV 123 Query: 123 VDCLDNFETRYLLDDYAHKVGIPLV 147 +D D + L K IP++ Sbjct: 124 IDASDTITYKIHLMKECLKRNIPII 148 >pir||T00587 probable ubiquitin--protein ligase (EC 6.3.2.19) - Arabidopsis thaliana >gi|1750376|gb|AAB39246.1| (U80808) ubiquitin activating enzyme [Arabidopsis thaliana] >gi|3150409|gb|AAC16961.1| (AC004165) ubiquitin activating enzyme (UBA1) [Arabidopsis thaliana] Length = 1080 Score = 73.7 bits (178), Expect = 2e-12 Identities = 50/157 (31%), Positives = 82/157 (51%), Gaps = 7/157 (4%) Query: 4 ESELERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPE 63 E + + + RQ+ ++G E +L + V + G+ GLG+ +A L AGV + L DE+V E Sbjct: 72 EIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKNLILAGVKSVTLHDERVVE 131 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIV 123 L +L+ + E+D+G+N S + KL+ N+ V + + L +E+ L G V+V Sbjct: 132 LWDLSSNFVFSEDDVGKNRADASVQ-KLQDLNNAVVVSSLTKSLNKED----LSGFQVVV 186 Query: 124 DCLDNFETRYLLDDY--AHKVGIPLVHGAVEGLYGQV 158 + E DDY +H+ I V V GL+G V Sbjct: 187 FSDISMERAIEFDDYCHSHQPPIAFVKADVRGLFGSV 223 Score = 71.8 bits (173), Expect = 7e-12 Identities = 52/168 (30%), Positives = 81/168 (47%), Gaps = 10/168 (5%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV-----GRILLIDEQVPE 63 RYD QI +FG + Q+KL+ AKV VG G LG L GV G++ + D+ + E Sbjct: 474 RYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGVSCGSQGKLTVTDDDIIE 533 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKL---EKFNSDVKIETFVGKLTEENIEDVL-HGV 119 SNL+RQ L + ++G+ ++A +FN + ++ VG TE +D + Sbjct: 534 KSNLSRQFLFRDWNIGQAKSTVAASAAAVINPRFNIEA-LQNRVGAETENVFDDAFWENL 592 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 V+V+ LDN R +D PL+ G ++IP T+ Sbjct: 593 TVVVNALDNVNARLYVDSRCLYFQKPLLESGTLGTKCNTQSVIPHLTE 640 >pir||T20004 hypothetical protein C47E12.5 - Caenorhabditis elegans >gi|3875106|emb|CAA93101.1| (Z68882) predicted using Genefinder~Similarity to Mouse ubiquitin-activating enzyme (SW:UBA_MOUSE), contains similarity to Pfam domain: PF00899 (ThiF family), Score=374.3, E-value=4e-109, N=2; PF02134 (Repeat in ubiquitin-activating (UBA) proteins), Sc> Length = 1113 Score = 73.7 bits (178), Expect = 2e-12 Identities = 58/179 (32%), Positives = 84/179 (46%), Gaps = 14/179 (7%) Query: 2 LSESELE----RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV-----G 52 LSE++ + RYD Q +FG QE L + + VVG G +G + L+ GV G Sbjct: 488 LSETDCQPRQSRYDGQAAVFGWPYQECLFRQRWFVVGAGAIGCELLKNLSMMGVACGEGG 547 Query: 53 RILLIDEQVPELSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETF---VGKLTE 109 I + D E+SNLNRQ L D+G +A+ + FNSDV+IE VG TE Sbjct: 548 LIKITDMDQIEISNLNRQFLFRRRDVGGKKSECAAR-AVTAFNSDVRIEALAERVGLETE 606 Query: 110 ENIEDVLHG-VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 D G ++ + + LDN + R +D +PL+ G G + P T+ Sbjct: 607 HIFNDEFFGELNGVANALDNVDARRYMDRRCVYYRLPLLESGTMGTKGNTQVVYPYLTE 665 >gb|AAD18053.1| (AF124162) molybdopterin synthase sulphurylase [Nicotiana plumbaginifolia] Length = 140 Score = 73.4 bits (177), Expect = 2e-12 Identities = 37/71 (52%), Positives = 47/71 (66%), Gaps = 2/71 (2%) Query: 2 LSESELERYDRQIMI--FGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDE 59 LS + RY R +++ FGV+GQ L K+ V V+G GGLGSP YL A GVGRI ++D Sbjct: 70 LSPDMIYRYSRHLLLPSFGVQGQANLLKSSVLVIGAGGLGSPALLYLAACGVGRIGIVDH 129 Query: 60 QVPELSNLNRQ 70 V EL+NL RQ Sbjct: 130 DVVELNNLQRQ 140 >gi|6322639 ubiquitin activating enzyme, similar to Uba2p; Uba1p [Saccharomyces cerevisiae] >gi|549145|sp|P22515|UBA1_YEAST UBIQUITIN-ACTIVATING ENZYME E1 1 >gi|538616|pir||S38048 ubiquitin--protein ligase (EC 6.3.2.19) - yeast (Saccharomyces cerevisiae) >gi|486375|emb|CAA82055.1| (Z28210) ORF YKL210w [Saccharomyces cerevisiae] Length = 1024 Score = 73.0 bits (176), Expect = 3e-12 Identities = 54/170 (31%), Positives = 82/170 (47%), Gaps = 12/170 (7%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGR-----ILLIDEQVPE 63 RYD QI +FG++ Q+K+ +KV +VG G +G + G+G I++ D E Sbjct: 416 RYDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLGSGSDGYIVVTDNDSIE 475 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIE-----TFVGKLTEENIEDVL-H 117 SNLNRQ L +D+G+N ++A+ + N D+K + VG TEE D Sbjct: 476 KSNLNRQFLFRPKDVGKNKSEVAAE-AVCAMNPDLKGKINAKIDKVGPETEEIFNDSFWE 534 Query: 118 GVDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 +D + + LDN + R +D PL+ G G IIP T+ Sbjct: 535 SLDFVTNALDNVDARTYVDRRCVFYRKPLLESGTLGTKGNTQVIIPRLTE 584 >gi|6755923 ubiquitin activating enzyme E1, Chr Y 1 [Mus musculus] >gi|5929777|gb|AAD56603.1| (AF127490) ubiquitin activating enzyme E1 [Mus musculus] Length = 1058 Score = 73.0 bits (176), Expect = 3e-12 Identities = 46/149 (30%), Positives = 76/149 (50%), Gaps = 5/149 (3%) Query: 10 YDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNR 69 Y RQ+ + G E + L+ + V + G+ GLG +A + GV + L D+ + + ++L+ Sbjct: 54 YSRQLYVLGHEAMKHLQASSVLISGLQGLGVEIAKNIILGGVKAVTLHDQGIAQWADLSS 113 Query: 70 QILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCLDNF 129 Q EED+G+N +S +L + NS V + + G L IE+ L G V+V Sbjct: 114 QFCLREEDIGKNRAEISQP-RLAELNSYVPVFAYTGPL----IEEFLSGFQVVVLTNTPL 168 Query: 130 ETRYLLDDYAHKVGIPLVHGAVEGLYGQV 158 E + + ++ H GI LV GL GQ+ Sbjct: 169 EYQLQVGEFCHSHGIKLVVADTRGLVGQL 197 >gb|AAF00149.1|AF150963_1 (AF150963) ubiquitin-activating enzyme E1 [Mus musculus] Length = 1058 Score = 73.0 bits (176), Expect = 3e-12 Identities = 46/149 (30%), Positives = 76/149 (50%), Gaps = 5/149 (3%) Query: 10 YDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNR 69 Y RQ+ + G E + L+ + V + G+ GLG +A + GV + L D+ + + ++L+ Sbjct: 54 YSRQLYVLGHEAMKHLQASSVLISGLQGLGVEIAKNIILCGVKAVTLHDQGIAQWADLSS 113 Query: 70 QILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCLDNF 129 Q EED+G+N +S +L + NS V + + G L IE+ L G V+V Sbjct: 114 QFCLREEDIGKNRAEISQP-RLAELNSYVPVFAYTGPL----IEEFLSGFQVVVLTNTPL 168 Query: 130 ETRYLLDDYAHKVGIPLVHGAVEGLYGQV 158 E + + ++ H GI LV GL GQ+ Sbjct: 169 EYQLQVGEFCHSHGIKLVVADTRGLVGQL 197 >gb|AAF31704.1|AF218864_1 (AF218864) Smt3 activating enzyme 2 [Drosophila melanogaster] Length = 700 Score = 73.0 bits (176), Expect = 3e-12 Identities = 54/162 (33%), Positives = 80/162 (49%), Gaps = 4/162 (2%) Query: 23 EKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDLGRNP 82 E +KK+KV VVG GG+G V L +G I +ID +LSNLNRQ L E +G++ Sbjct: 15 ELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIIDLDTIDLSNLNRQFLFHREHVGKS- 73 Query: 83 KPLSAKWKLEKFNSDVKIETFVGKLTEENI-EDVLHGVDVIVDCLDNFETRYLLDDYAHK 141 K A+ FN D KI + +T + + D++++ LDN R ++ Sbjct: 74 KARVARESALSFNPDAKITAYHDSVTSTDYGVNFFKKFDLVLNALDNRADRNHVNRMCLN 133 Query: 142 VGIPLVHGAVEGLYGQVTTIIPGKTKRLREIFPRIKKKGKFP 183 +PL+ G GQV I G T + E P+ K++ FP Sbjct: 134 ADVPLIESGTAGYNGQVELIKRGLT-QCYECTPKDKQR-SFP 173 >gb|AAG03059.1|AF288693_1 (AF288693) Ube1l [Mus musculus] Length = 775 Score = 73.0 bits (176), Expect = 3e-12 Identities = 47/151 (31%), Positives = 77/151 (50%), Gaps = 5/151 (3%) Query: 8 ERYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNL 67 E Y RQ+ + G+ +++++AKV + G+ GLG+ VA L GVG + L D ++L Sbjct: 4 ELYSRQLYVLGLPAMQRIQEAKVLLCGLQGLGAEVAKNLVLTGVGSLTLHDPHPTCWADL 63 Query: 68 NRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVDVIVDCLD 127 Q EE LGRN + +++ +L + N V+I G +T ED+L G V+V Sbjct: 64 AAQCFLSEESLGRN-RAEASQAQLAQLNEAVQISVHSGDIT----EDLLQGFQVVVLTDS 118 Query: 128 NFETRYLLDDYAHKVGIPLVHGAVEGLYGQV 158 E + + HK G+ + GL G++ Sbjct: 119 KLEDQLKVGPLCHKHGVRFLMAETRGLVGRL 149 >emb|CAA39056.1| (X55386) ubiquitin-activating enzyme [Saccharomyces cerevisiae] Length = 1024 Score = 71.4 bits (172), Expect = 9e-12 Identities = 53/170 (31%), Positives = 82/170 (48%), Gaps = 12/170 (7%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGR-----ILLIDEQVPE 63 RYD QI +FG++ Q+K+ +KV +VG G +G + G+G I++ D E Sbjct: 416 RYDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLGSGSDGYIVVTDNDSIE 475 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIE-----TFVGKLTEENIEDVL-H 117 SNLNRQ L +D+G+N ++A+ + N D+K + VG TE+ D Sbjct: 476 KSNLNRQFLFRPKDVGKNKSEVAAE-AVCAMNPDLKGKINAKIDKVGPETEKIFNDSFWE 534 Query: 118 GVDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 +D + + LDN + R +D PL+ G G IIP T+ Sbjct: 535 SLDFVTNALDNVDARTYVDRRCVFYRKPLLESGTLGTKGNTQVIIPRLTE 584 >emb|CAB54319.2| (Z82062) contains similarity to Pfam domain: PF00899 (ThiF family), Score=156.7, E-value=1.3e-43, N=1; PF02134 (Repeat in ubiquitin-activating (UBA) proteins), Score=97.6, E-value=3.1e-26, N=1 [Caenorhabditis elegans] Length = 438 Score = 71.4 bits (172), Expect = 9e-12 Identities = 41/149 (27%), Positives = 77/149 (51%), Gaps = 2/149 (1%) Query: 20 EGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDLG 79 E EK+ ++K+ V+G GG+G + L G ++ +ID ++SNLNRQ L +E + Sbjct: 6 EKHEKIVQSKILVIGAGGIGCELLKNLAVTGFRKVHVIDLDTIDISNLNRQFLFRKEHVS 65 Query: 80 RNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIE-DVLHGVDVIVDCLDNFETRYLLDDY 138 + K +A +++F +++ + E+ + D++++ LDN R ++ Sbjct: 66 -SSKAATATQVVKQFCPQIELTFDHDSIFEKKYNMEFFQAYDIVLNALDNRAARNYVNRM 124 Query: 139 AHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 H PL+ G +GQV+ I+ GKT+ Sbjct: 125 CHAANRPLIDSGSGGYFGQVSVIMRGKTE 153 >pir||T51083 related to ubiquitin-activating enzyme homolog UBA2 [imported] - Neurospora crassa >gi|9368587|emb|CAB98247.1| (AL390092) related to ubiquitin-activating enzyme homolog UBA2 [Neurospora crassa] Length = 641 Score = 70.6 bits (170), Expect = 2e-11 Identities = 47/146 (32%), Positives = 69/146 (47%), Gaps = 12/146 (8%) Query: 27 KAKVAVVGVGGLGSPVAYYLTAAGVGRILLIDEQVPELSNLNRQILHWEEDLGRNPKPLS 86 +A+V +VG GG+G + L G G + ++D +LSNLNRQ L E + + K L Sbjct: 28 QARVLMVGAGGIGCELLKNLVLTGFGEVHVVDLDTIDLSNLNRQFLFRHEHI-KKSKALV 86 Query: 87 AKWKLEKFNSDVKIETFVGKLTEENIEDVLHGVD------VIVDCLDNFETRYLLDDYAH 140 AK +KFN VKI NI+D ++ ++ + LDN E R ++ Sbjct: 87 AKEAAQKFNPAVKIVAH-----HANIKDAQFNIEWFSSFRIVFNALDNLEARRHVNKMCL 141 Query: 141 KVGIPLVHGAVEGLYGQVTTIIPGKT 166 +PL+ G GQV I G T Sbjct: 142 AADVPLIESGTTGFNGQVQVIKKGVT 167 >sp|P20973|UBA1_WHEAT UBIQUITIN-ACTIVATING ENZYME E1 1 >gi|100841|pir||A38373 ubiquitin--protein ligase (EC 6.3.2.19) E1 - wheat >gi|285451|pir||A42873 ubiquitin-activating enzyme E1, UBA1 - Wheat >gi|170780|gb|AAA34308.1| (M55604) ubiquitin-activating enzyme E1 [Triticum aestivum] Length = 1051 Score = 70.2 bits (169), Expect = 2e-11 Identities = 52/169 (30%), Positives = 79/169 (45%), Gaps = 12/169 (7%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV-----GRILLIDEQVPE 63 RYD QI +FG + Q KL++AK+ +VG G LG L G+ G + L D+ V E Sbjct: 444 RYDAQISVFGSKLQNKLEEAKIFMVGSGALGCEFLKNLALMGISCSQNGNLTLTDDDVIE 503 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLH-----G 118 SNL+RQ L + ++G+ PK A N + +E + + E E+V + Sbjct: 504 KSNLSRQFLFRDWNIGQ-PKSTVAATAAMVINPKLHVEALQNRASPET-ENVFNDAFWEN 561 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 +D +V+ LDN R +D PL+ G +IP T+ Sbjct: 562 LDAVVNALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQMVIPHLTE 610 >sp|P31252|UBA3_WHEAT UBIQUITIN-ACTIVATING ENZYME E1 3 >gi|7437981|pir||T06483 probable ubiquitin--protein ligase (EC 6.3.2.19) E1 - wheat >gi|170686|gb|AAA34266.1| (M90664) ubiquitin activating enzyme [Triticum aestivum] Length = 1053 Score = 69.9 bits (168), Expect = 3e-11 Identities = 49/169 (28%), Positives = 80/169 (46%), Gaps = 12/169 (7%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV-----GRILLIDEQVPE 63 RYD Q+ +FG + Q+K+++A VVG G LG L GV G++ + D+ + E Sbjct: 447 RYDAQVSVFGSKLQKKMEEANTFVVGSGALGCEFLKNLALMGVSCSSKGKLTITDDDIIE 506 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLH-----G 118 SNL+RQ L + ++G+ K A N + I+ + + E+V H G Sbjct: 507 KSNLSRQFLFRDWNIGQ-AKSTVAATAASAINPSLHIDALQNRACPDT-ENVFHDTFWEG 564 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 +DV+++ LDN R +D PL+ G + +IP T+ Sbjct: 565 LDVVINALDNVNARMYMDMRCLYFQKPLLESGTLGAKCNIQMVIPHLTE 613 >pir||A48195 ubiquitin--protein ligase E1 homolog - human Length = 1058 Score = 68.7 bits (165), Expect = 6e-11 Identities = 53/172 (30%), Positives = 81/172 (46%), Gaps = 12/172 (6%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGR-----ILLIDEQVPE 63 RYD QI +FG QEKL++ +VG G +G + G+G + ++D E Sbjct: 460 RYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIE 519 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKL--TEENI--EDVLHGV 119 SNL+RQ L +D+GR PK A N D+++ L T E+I ++ V Sbjct: 520 RSNLSRQFLFRSQDVGR-PKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRV 578 Query: 120 DVIVDCLDNFET-RYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLR 170 D + LD+F+ RY+ H + PL+ G +G T +P T+ R Sbjct: 579 DGVAAALDSFQARRYVAARCTHYLK-PLLEAGTSGTWGSATVFMPHVTEAYR 629 >gi|4507767 ubiquitin-activating enzyme E1-like; Ubiquitin-activating enzyme-2; ubiquitin-activating enzyme E1, like [Homo sapiens] >gi|731039|sp|P41226|UBAL_HUMAN UBIQUITIN-ACTIVATING ENZYME E1 HOMOLOG (D8) >gi|986881|gb|AAA75388.1| (L13852) ubiquitin-activating enzyme E1-related protein [Homo sapiens] Length = 1011 Score = 68.7 bits (165), Expect = 6e-11 Identities = 53/172 (30%), Positives = 81/172 (46%), Gaps = 12/172 (6%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGVGR-----ILLIDEQVPE 63 RYD QI +FG QEKL++ +VG G +G + G+G + ++D E Sbjct: 413 RYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIE 472 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKL--TEENI--EDVLHGV 119 SNL+RQ L +D+GR PK A N D+++ L T E+I ++ V Sbjct: 473 RSNLSRQFLFRSQDVGR-PKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRV 531 Query: 120 DVIVDCLDNFET-RYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTKRLR 170 D + LD+F+ RY+ H + PL+ G +G T +P T+ R Sbjct: 532 DGVAAALDSFQARRYVAARCTHYLK-PLLEAGTSGTWGSATVFMPHVTEAYR 582 >pir||T03964 probable ubiquitin--protein ligase (EC 6.3.2.19) - common tobacco >gi|1808656|emb|CAA71762.1| (Y10804) Ubiquitin activating enzyme E1 [Nicotiana tabacum] Length = 1080 Score = 68.7 bits (165), Expect = 6e-11 Identities = 51/168 (30%), Positives = 78/168 (46%), Gaps = 10/168 (5%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV-----GRILLIDEQVPE 63 RYD QI +FG + Q+KL++AK VVG G LG L GV G++ + D+ V E Sbjct: 474 RYDAQISVFGNKLQKKLEEAKAFVVGSGALGCEFLKNLALMGVCCGDKGKLTITDDDVIE 533 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKL---TEENIEDVL-HGV 119 SNL+RQ L + ++G+ ++A N + IE + TE +D + Sbjct: 534 KSNLSRQFLFRDWNIGQAKSTVAAA-AASLINPRIHIEALQNRASPETESVFDDTFWENL 592 Query: 120 DVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 V+++ LDN R +D PL+ G +IP T+ Sbjct: 593 SVVINALDNVNARLYIDQRCLYFQKPLLESGTLGAKCNTQMVIPHLTE 640 >sp|P31251|UBA2_WHEAT UBIQUITIN-ACTIVATING ENZYME E1 2 >gi|170684|gb|AAA34265.1| (M90663) ubiquitin activating enyme [Triticum aestivum] Length = 1051 Score = 68.3 bits (164), Expect = 8e-11 Identities = 51/169 (30%), Positives = 78/169 (45%), Gaps = 12/169 (7%) Query: 9 RYDRQIMIFGVEGQEKLKKAKVAVVGVGGLGSPVAYYLTAAGV-----GRILLIDEQVPE 63 RYD QI +FG Q KL++AK+ +VG G LG L G+ G + + D+ V E Sbjct: 444 RYDAQISVFGSTLQNKLEEAKIFMVGSGALGCEFLKNLALMGISCSQNGNLTVTDDDVIE 503 Query: 64 LSNLNRQILHWEEDLGRNPKPLSAKWKLEKFNSDVKIETFVGKLTEENIEDVLH-----G 118 SNL+RQ L + ++G+ PK A N + +E + + E E+V + Sbjct: 504 KSNLSRQFLFRDWNIGQ-PKSTVAATAAMVINPKLHVEALQNRASPET-ENVFNDAFWEN 561 Query: 119 VDVIVDCLDNFETRYLLDDYAHKVGIPLVHGAVEGLYGQVTTIIPGKTK 167 +D +V+ LDN R +D PL+ G +IP T+ Sbjct: 562 LDAVVNALDNVTARMYIDSRCVYFQKPLLESGTLGAKCNTQMVIPHLTE 610 Database: ./suso.pep Posted date: Jul 6, 2001 5:57 PM Number of letters in database: 840,471 Number of sequences in database: 2977 Database: /banques/blast2/nr.pep Posted date: Dec 14, 2000 12:46 PM Number of letters in database: 188,266,275 Number of sequences in database: 595,510 Lambda K H 0.319 0.142 0.403 Gapped Lambda K H 0.270 0.0470 0.230 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 84603704 Number of Sequences: 2977 Number of extensions: 3650550 Number of successful extensions: 11397 Number of sequences better than 1.0e-10: 102 Number of HSP's better than 0.0 without gapping: 37 Number of HSP's successfully gapped in prelim test: 65 Number of HSP's that attempted gapping in prelim test: 11133 Number of HSP's gapped (non-prelim): 135 length of query: 230 length of database: 189,106,746 effective HSP length: 54 effective length of query: 176 effective length of database: 156,788,448 effective search space: 27594766848 effective search space used: 27594766848 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.8 bits) X3: 64 (24.9 bits) S1: 41 (21.7 bits) S2: 163 (67.9 bits)