BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2338 (PAB2338) DE:PYRUVATE FORMATE-LYASE ACTIVATING ENZYME
RELATED PROTEIN
(240 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75187 pyruvate formate-lyase activating enzyme related pro... 506 e-142
pir||A71220 hypothetical protein PH0020 - Pyrococcus horikoshii ... 307 8e-83
pir||F69078 probable pyruvate formate-lyase activating enzyme (E... 83 3e-15
pir||B64453 probable pyruvate formate-lyase activating enzyme (E... 76 5e-13
>pir||B75187 pyruvate formate-lyase activating enzyme related protein PAB2338 -
Pyrococcus abyssi (strain Orsay)
>gi|5457454|emb|CAB48945.1| (AJ248283) PYRUVATE
FORMATE-LYASE ACTIVATING ENZYME RELATED PROTEIN
[Pyrococcus abyssi]
Length = 240
Score = 506 bits (1289), Expect = e-142
Identities = 240/240 (100%), Positives = 240/240 (100%)
Query: 1 MLTAGWKSISMVDVHGKVTFTIWLCGCNLKCPFCHNWRIADRLECHELNVNSLMEDLSQS 60
MLTAGWKSISMVDVHGKVTFTIWLCGCNLKCPFCHNWRIADRLECHELNVNSLMEDLSQS
Sbjct: 1 MLTAGWKSISMVDVHGKVTFTIWLCGCNLKCPFCHNWRIADRLECHELNVNSLMEDLSQS 60
Query: 61 SLFIDYFHITGGEPLVQWRELSDLLRKVEEIGVEVSLNTNLTIVRPLERLLSEGLVNHIA 120
SLFIDYFHITGGEPLVQWRELSDLLRKVEEIGVEVSLNTNLTIVRPLERLLSEGLVNHIA
Sbjct: 61 SLFIDYFHITGGEPLVQWRELSDLLRKVEEIGVEVSLNTNLTIVRPLERLLSEGLVNHIA 120
Query: 121 TDLKVPPLELYGLPKNASNVLWKLYLKGLEIVSRHKIPLELRIPIPRGFNIEPWVKEVLP 180
TDLKVPPLELYGLPKNASNVLWKLYLKGLEIVSRHKIPLELRIPIPRGFNIEPWVKEVLP
Sbjct: 121 TDLKVPPLELYGLPKNASNVLWKLYLKGLEIVSRHKIPLELRIPIPRGFNIEPWVKEVLP 180
Query: 181 RIKTDYYVVLNPLVGRPLTNPRNEEWCNKHCWPRKEEISKIKEIIEEYGVNVYVSSFASS 240
RIKTDYYVVLNPLVGRPLTNPRNEEWCNKHCWPRKEEISKIKEIIEEYGVNVYVSSFASS
Sbjct: 181 RIKTDYYVVLNPLVGRPLTNPRNEEWCNKHCWPRKEEISKIKEIIEEYGVNVYVSSFASS 240
>pir||A71220 hypothetical protein PH0020 - Pyrococcus horikoshii
>gi|3256405|dbj|BAA29088.1| (AP000001) 238aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 238
Score = 307 bits (778), Expect = 8e-83
Identities = 140/241 (58%), Positives = 185/241 (76%), Gaps = 8/241 (3%)
Query: 1 MLTAGWKSISMVDVHGKVTFTIWLCGCNLKCPFCHNWRIADRLECHELNVNSLMEDLSQS 60
ML +GWK +SMVDVHGK TFT+WLCGCNL+CPFCHNWRIA C +LN L+ ++ +
Sbjct: 1 MLVSGWKEVSMVDVHGKTTFTLWLCGCNLRCPFCHNWRIAQGEGCFKLNREELIAEVDAN 60
Query: 61 SLFIDYFHITGGEPLVQWRELSDLLRKVEEIGVEVSLNTNLTIVRPLERLLSEGLVNHIA 120
S +D FHITGGEPL+QW+EL +LL V + +SLN+NLT+V+PLER++ ++H+A
Sbjct: 61 SFLVDCFHITGGEPLIQWKELRNLLVDVRRY-LPISLNSNLTLVKPLERVIE--FLDHVA 117
Query: 121 TDLKVPPLELYGLPKNASNVLWKLYLKGLEIVSRHKIPLELRIPIPRGFNIE---PWVKE 177
TDLKVPP ELYGLP+ +S LWKL+L GL IVS + IPLELRIP+ RGF +E PW++E
Sbjct: 118 TDLKVPPTELYGLPRESSIKLWKLFLDGLSIVSNYSIPLELRIPVSRGFKVEDIKPWIEE 177
Query: 178 VLPRIKTDYYVVLNPLVGRPLTNPRNEEWCNKHCWPRKEEISKIKEIIEEYGV-NVYVSS 236
+ RI TD+YVVLNPLVG PLT+PR++EWC +HCWPR E+ K+K++++ G+ V V S
Sbjct: 178 GIERINTDFYVVLNPLVGPPLTDPRDKEWCAEHCWPR-NEVEKLKDLLKSLGIEKVIVKS 236
Query: 237 F 237
+
Sbjct: 237 Y 237
>pir||F69078 probable pyruvate formate-lyase activating enzyme (EC 1.97.4.1)
MTH1586 - Methanobacterium thermoautotrophicum (strain
Delta H) >gi|2622709|gb|AAB86059.1| (AE000918) pyruvate
formate-lyase activating enzyme [Methanobacterium
thermoautotrophicum]
Length = 233
Score = 83.1 bits (202), Expect = 3e-15
Identities = 51/185 (27%), Positives = 97/185 (51%), Gaps = 8/185 (4%)
Query: 5 GWKSISMVDVHGKVTFTIWLCGCNLKCPFCHNWRIADRLECHELNVNSLMEDLSQSSLFI 64
G ++S +D GK + I+ GCN +CP+CHN + D + E+++ ++++D+ + S F+
Sbjct: 7 GSMAVSTLDYPGKTSLVIFTAGCNFRCPYCHNPELIDGGD--EVDLETVLDDVERYSEFV 64
Query: 65 DYFHITGGEPLVQWRELSDLLRKVEEIGVEVSLNTNLTIVRPLERLLSEGLVNHIATDLK 124
+ ++GGEPL+Q L +L +G+ L+TN + LE ++++A D+K
Sbjct: 65 EALVVSGGEPLLQVDALETVLEHARSLGLATKLDTNGSYPEALEGYCP--TLDYVAIDVK 122
Query: 125 VPPLELYGLPKNASNVLWKLYLKGLEIVSRHKIPLELRIPIPRGFNIEPWVKEVLPRIKT 184
P L+ P+ A + ++ LEI+ + +E R G E ++ + I+
Sbjct: 123 AP---LHRYPELAGACAGGV-MRSLEILRDSGVTVECRTTFVPGLMGEEDIEMIAESIEC 178
Query: 185 DYYVV 189
D YV+
Sbjct: 179 DVYVL 183
>pir||B64453 probable pyruvate formate-lyase activating enzyme (EC 1.97.4.1) -
Methanococcus jannaschii >gi|1591858|gb|AAB99230.1|
(U67563) pyruvate formate-lyase activating enzyme (act)
[Methanococcus jannaschii]
Length = 240
Score = 75.7 bits (183), Expect = 5e-13
Identities = 59/239 (24%), Positives = 115/239 (47%), Gaps = 17/239 (7%)
Query: 2 LTAGWKSISMVDVHGKVTFTIWLCGCNLKCPFCHNWRIADRLECHELNVNSLMEDLSQSS 61
L +G +S +D K + I+L GCN+KCP+CHN + + + V + D+
Sbjct: 4 LVSGIVDLSTIDYPKKASAVIFLYGCNMKCPYCHNLKFMLEHK-RGMTVEEIFNDID--F 60
Query: 62 LFIDYFHITGGEPLVQWRELSDLLRKVEEIGVEVSLNTNLTIVRPLERLLSEGLVNHIAT 121
LF D I+GGEP +Q + ++ R +E G V ++TN T +E L+ L++++A
Sbjct: 61 LFADAIVISGGEPTLQKDAVIEIARYAKEKGFPVKIDTNGTHPEVIEELIKNKLIDYVAI 120
Query: 122 DLKV---PPLELYGLPKNASNVLWKLYLKGLEIVSRHKIPLELRIPIPRGFNIEPWVKEV 178
D+K E ++ + K+ LK +++ ++ + +E R E ++++
Sbjct: 121 DVKCRFDKYKEFVKCREDGEEIKNKI-LKIIDLCKKNNVFVECRTTFVPKVMDEEDIEDI 179
Query: 179 LPRIK-TDYYVVLNPLVGRPLTNPRN--EEWCNKHCWPRKEEISKIKEIIEEYGVNVYV 234
+K D Y + P++ +E K P++ E+ ++ +I ++Y NV +
Sbjct: 180 AKTVKDCDLYAIQQ-------FEPKDAYDEEFKKLPMPKENELRELGKIAKKYIDNVVI 231
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.140 0.445
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96220091
Number of Sequences: 2977
Number of extensions: 4224677
Number of successful extensions: 9236
Number of sequences better than 1.0e-10: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 9228
Number of HSP's gapped (non-prelim): 4
length of query: 240
length of database: 189,106,746
effective HSP length: 49
effective length of query: 191
effective length of database: 159,780,883
effective search space: 30518148653
effective search space used: 30518148653
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 164 (68.3 bits)