BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2355 (bioB-like) DE:BIOTIN SYNTHASE (EC 2.8.1.6) RELATED
PROTEIN
(302 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||C75211 biotin synthase (EC 2.8.1.6) related protein PAB2355... 622 e-177
pir||C71247 hypothetical protein PH0234 - Pyrococcus horikoshii ... 479 e-134
pir||D72398 conserved hypothetical protein - Thermotoga maritima... 183 2e-45
gb|AAK41371.1| Biotin synthase (bioB) [Sulfolobus solfataricus] 182 6e-45
pir||F70467 hypothetical protein aq_1958 - Aquifex aeolicus >gi|... 147 1e-34
>pir||C75211 biotin synthase (EC 2.8.1.6) related protein PAB2355 - Pyrococcus
abyssi (strain Orsay) >gi|5457647|emb|CAB49138.1|
(AJ248283) BIOTIN SYNTHASE (EC 2.8.1.6) RELATED PROTEIN
[Pyrococcus abyssi]
Length = 302
Score = 622 bits (1586), Expect = e-177
Identities = 302/302 (100%), Positives = 302/302 (100%)
Query: 1 MRVRLSYGTAVSMGLIRAKLLARPTTAYLMTYYEGRCINDCKFCAQARSSRANLEMLSRV 60
MRVRLSYGTAVSMGLIRAKLLARPTTAYLMTYYEGRCINDCKFCAQARSSRANLEMLSRV
Sbjct: 1 MRVRLSYGTAVSMGLIRAKLLARPTTAYLMTYYEGRCINDCKFCAQARSSRANLEMLSRV 60
Query: 61 VWPAFELETVLKNFSKGNFKRICLQTIDYPNMLSDVFSLLEELSKLNVPISLSITPVPRS 120
VWPAFELETVLKNFSKGNFKRICLQTIDYPNMLSDVFSLLEELSKLNVPISLSITPVPRS
Sbjct: 61 VWPAFELETVLKNFSKGNFKRICLQTIDYPNMLSDVFSLLEELSKLNVPISLSITPVPRS 120
Query: 121 ELEEFSTLGVDYIGVGLDAASERVYKDVKISRYSWDDMWKFFDDVLSVFGKGRAVVHLIV 180
ELEEFSTLGVDYIGVGLDAASERVYKDVKISRYSWDDMWKFFDDVLSVFGKGRAVVHLIV
Sbjct: 121 ELEEFSTLGVDYIGVGLDAASERVYKDVKISRYSWDDMWKFFDDVLSVFGKGRAVVHLIV 180
Query: 181 GLGETDKEIVETIGEVYKKGGIVSLFAFTPIKGTQMENFSPPPLERYRKIQVAHYLIKTG 240
GLGETDKEIVETIGEVYKKGGIVSLFAFTPIKGTQMENFSPPPLERYRKIQVAHYLIKTG
Sbjct: 181 GLGETDKEIVETIGEVYKKGGIVSLFAFTPIKGTQMENFSPPPLERYRKIQVAHYLIKTG 240
Query: 241 EAELEDFEFDEFGNLIGLPKTEVPPIAYVTQGCPWCNRPYYNERPGREPYNYPSVEMVRK 300
EAELEDFEFDEFGNLIGLPKTEVPPIAYVTQGCPWCNRPYYNERPGREPYNYPSVEMVRK
Sbjct: 241 EAELEDFEFDEFGNLIGLPKTEVPPIAYVTQGCPWCNRPYYNERPGREPYNYPSVEMVRK 300
Query: 301 RK 302
RK
Sbjct: 301 RK 302
>pir||C71247 hypothetical protein PH0234 - Pyrococcus horikoshii
>gi|3256623|dbj|BAA29306.1| (AP000001) 311aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 311
Score = 479 bits (1220), Expect = e-134
Identities = 212/299 (70%), Positives = 259/299 (85%)
Query: 1 MRVRLSYGTAVSMGLIRAKLLARPTTAYLMTYYEGRCINDCKFCAQARSSRANLEMLSRV 60
M++R+SYGTAV+MGLIRAK++ +PTTAYLMTY++G+CINDCKFCAQARSS+ANL+MLSR+
Sbjct: 1 MKIRVSYGTAVAMGLIRAKIVVKPTTAYLMTYHDGKCINDCKFCAQARSSKANLKMLSRI 60
Query: 61 VWPAFELETVLKNFSKGNFKRICLQTIDYPNMLSDVFSLLEELSKLNVPISLSITPVPRS 120
WPAF +E V+KNF G+FKR+CLQTIDYP ++ D++ +LE LS L PIS+SITPV +
Sbjct: 61 SWPAFNIEDVIKNFHNGSFKRVCLQTIDYPGLIDDIWGILERLSVLKTPISISITPVSKD 120
Query: 121 ELEEFSTLGVDYIGVGLDAASERVYKDVKISRYSWDDMWKFFDDVLSVFGKGRAVVHLIV 180
L EF GVDYIG+GLDAASE +Y++VKISR+SWDDMW F +DV+SVFG+GR VVH+IV
Sbjct: 121 ILMEFKKFGVDYIGIGLDAASEEIYRNVKISRHSWDDMWMFLEDVVSVFGRGRGVVHIIV 180
Query: 181 GLGETDKEIVETIGEVYKKGGIVSLFAFTPIKGTQMENFSPPPLERYRKIQVAHYLIKTG 240
GLGE D++++ETI VYKKGGIVSLFAFTP+KGTQMEN+ PP +ERYRKIQ AHYLI+TG
Sbjct: 181 GLGEKDRDLIETIFRVYKKGGIVSLFAFTPLKGTQMENYPPPSIERYRKIQAAHYLIRTG 240
Query: 241 EAELEDFEFDEFGNLIGLPKTEVPPIAYVTQGCPWCNRPYYNERPGREPYNYPSVEMVR 299
+ E DFEFDE GNLI LP ++PP A++T GCPWCNRPYYNERPGREPYNYPS+EM R
Sbjct: 241 KVEFSDFEFDERGNLIHLPSYDIPPSAFITYGCPWCNRPYYNERPGREPYNYPSLEMAR 299
>pir||D72398 conserved hypothetical protein - Thermotoga maritima (strain MSB8)
>gi|4980742|gb|AAD35334.1|AE001708_2 (AE001708)
conserved hypothetical protein [Thermotoga maritima]
Length = 283
Score = 183 bits (460), Expect = 2e-45
Identities = 109/292 (37%), Positives = 161/292 (54%), Gaps = 14/292 (4%)
Query: 1 MRVRLSYGTAVSMGLIRAKLLARPTTAYLMTYYEGRCINDCKFCAQARSSRANLEMLSRV 60
M +R SYGT + + TAYLM RC+ +C +CAQ+RSS + LSRV
Sbjct: 1 MMLRASYGT---IRKVNGNSSLEMDTAYLML--GERCVYNCLYCAQSRSSTSPSHFLSRV 55
Query: 61 VWPAFELETVLKNFSKGNFKRICLQTIDYPNMLSDVFSLLEELSKLNVPISLSITPVPRS 120
W E+ L FKR+C+Q + YP+ D+ ++ + +P+S+S+ V
Sbjct: 56 TWK--EVSEDLLGKLNDRFKRVCIQVVSYPSYGKDLRLIIP---RFRIPVSVSVRAVSIE 110
Query: 121 ELEEFSTLGVDYIGVGLDAASERVYKDVKISRYSWDDMWKFFDDVLSVFGKGRAVVHLIV 180
E+ E+ LG D +G+ +D ++ +++ ++ +Y + + V +F GR H+IV
Sbjct: 111 EVVEYFELGADRVGIAVDVPNKTLFEKIRGGKY--ERHLHILEKVSEMF-PGRITTHVIV 167
Query: 181 GLGETDKEIVETIGEVYKKGGIVSLFAFTPIKGTQMENFSPPPLERYRKIQVAHYLIKTG 240
GLGE+DK+IV+ ++ +VSLFAFTPIKGT EN P LERYRKIQ+ YL++
Sbjct: 168 GLGESDKDIVDFTVWARERNIVVSLFAFTPIKGTAFENRERPSLERYRKIQLVTYLLEKN 227
Query: 241 EAELEDFEFDEFGNLIGLP-KTEVPPIAYVTQGCPWCNRPYYNERPGREPYN 291
+ E+ FD G +I + E P A T+GCP C RPYYNE P YN
Sbjct: 228 LIKPENIIFDSNGKIIDVEWNGEFPEEALRTRGCPHCTRPYYNESPRGPIYN 279
>gb|AAK41371.1| Biotin synthase (bioB) [Sulfolobus solfataricus]
Length = 351
Score = 182 bits (456), Expect = 6e-45
Identities = 110/315 (34%), Positives = 166/315 (51%), Gaps = 25/315 (7%)
Query: 3 VRLSYGTAVSMGLIRAKLL--ARPTTAYLMTYYEGRCINDCKFCAQAR--SSRANLEMLS 58
VRLS+G + +G L A TT L+ YY C +C +C QAR ++ + L
Sbjct: 12 VRLSFGADMVLGFSSGVFLKGALNTTINLLQYYPDGCKANCSYCGQAREVANGPECKTLI 71
Query: 59 RVVWPAFELETVLKNF--SKGN----FKRICLQTIDYPNMLSDVFSLLEELSKLNVPISL 112
RV WP L VLK +GN +RIC+ + +P D + + + + +
Sbjct: 72 RVEWPLRPLNEVLKRIYERQGNPEYGLQRICIGQLAHPRASPDAIEITRRIRNAGIELQI 131
Query: 113 S----ITPVPRSELEEFSTLGVDYIGVGLDAASERVYKDVK----ISRYSWDDMWKFFDD 164
S T + + E G D I V +DAA+E+V+++++ S +SW + D+
Sbjct: 132 SELVTATYTFKHHMIEMRKAGADMIDVAIDAANEKVFEELRGKKARSMHSWKRYLEAIDE 191
Query: 165 VLSVFGKGRAVVHLIVGLGETDKEIVETIGEVYKKGGIVSLFAFTPIKGTQMENFSPPPL 224
+ VFGK A +HLI+GLGE++K+ V + + +G +SLFAF P GT ME P P+
Sbjct: 192 AVEVFGKKNAGIHLIIGLGESEKDAVNLMWYAHSRGAKISLFAFYPEAGTPMEKRKPVPV 251
Query: 225 ERYRKIQVAHYLIKTGEAELEDFEFDEFGNLI------GLPKTEVPPIAYVTQGCPWCNR 278
YR++Q+A +LI+ ++ F+FD+ G LI + E+ P A++T GCP CNR
Sbjct: 252 HVYRRMQIARWLIENDIVDINAFKFDDKGELIDVEIPSDITLNELAP-AFMTSGCPGCNR 310
Query: 279 PYYNERPGREPYNYP 293
PY NERPGR N P
Sbjct: 311 PYSNERPGRVLKNVP 325
>pir||F70467 hypothetical protein aq_1958 - Aquifex aeolicus
>gi|2984211|gb|AAC07741.1| (AE000765) putative protein
[Aquifex aeolicus]
Length = 372
Score = 147 bits (368), Expect = 1e-34
Identities = 93/305 (30%), Positives = 157/305 (50%), Gaps = 27/305 (8%)
Query: 3 VRLSYGTAVSMGLIRAKLLARPTTAYLMTY--YEGRCINDCKFCA--QARSSRANLEMLS 58
+++S+ A+++GLI + + + T Y C C +C +AR + +
Sbjct: 19 MQISWAAAMTLGLIPGQFYRDTKLSCINTLLTYPSGCHATCAYCGLQKAREIEYSKKNFI 78
Query: 59 RVVWPAFELETVLKNFSK-GNFKRICLQTIDYPNMLSDVFSLLEEL-----SKLNVPISL 112
RV WP + + +++ + G+ +R+C+ I +P + D +LE++ K+ + I L
Sbjct: 79 RVEWPTVKTDEIIERTKQVGHAERLCISQITHPRAIRDTKYILEKVHRELGDKIFISILL 138
Query: 113 SITPVPRSELEEFSTLGVDYIGVGLDAASERVYKDVK----ISRYSWDDMWKFFDDVLSV 168
+ T +LE++ LG + + V LDAA+ +++ ++ S + W+ WK + +
Sbjct: 139 NATGHTYEDLEDYKRLGAETLTVALDAATPELFEKLRGRPMNSPHRWETYWKVLEWCADI 198
Query: 169 FGKGRAVVHLIVGLGETDKEIVETIGEVYKKGGIVSLFAFTPIKGTQMENFSPPPLERYR 228
G G HLIVGLGET++E+V TI V GG LF+F P +G+ ME P P +YR
Sbjct: 199 MGDGHVGAHLIVGLGETEEEMVRTIQRVRDLGGRTHLFSFWPEEGSLMEKEKPCPAPQYR 258
Query: 229 KIQVAHYLIKTGEAELEDFEFDEFGNLI--GLPKTEVPPI-----AYVTQGCPW------ 275
++Q+A YLI G A ED +F+E G +I G+ K I ++T GC
Sbjct: 259 RVQMARYLIDNGLARYEDMKFNEKGQIIDWGVSKEVFEEIFWSGRPFMTAGCRGKTTEVA 318
Query: 276 CNRPY 280
CNRP+
Sbjct: 319 CNRPF 323
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.321 0.139 0.420
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 115107183
Number of Sequences: 2977
Number of extensions: 4862350
Number of successful extensions: 13032
Number of sequences better than 1.0e-10: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 13018
Number of HSP's gapped (non-prelim): 5
length of query: 302
length of database: 189,106,746
effective HSP length: 53
effective length of query: 249
effective length of database: 157,386,935
effective search space: 39189346815
effective search space used: 39189346815
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 165 (68.7 bits)