BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2361 (metE-like2) DE:TETRAHYDROPTEROYLTRIGLUTAMATE
METHYLTRANSFERASE
(313 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75138 tetrahydropteroyltriglutamate methyltransferase PAB2... 622 e-177
pir||G71103 hypothetical protein PH1090 - Pyrococcus horikoshii ... 527 e-149
gb|AAK40735.1| 5-methyltetrahydropteroyltriglutamate- homocystei... 157 1e-37
gb|AAK40736.1| 5-methyltetrahydropteroyltriglutamate- homocystei... 116 2e-25
pir||D72509 hypothetical protein APE2050 - Aeropyrum pernix (str... 103 2e-21
pir||C75138 probable 5-methyltetrahydropteroyltriglutamate- homo... 80 4e-14
pir||B72509 probable 5-methltetrahydropteroyltriglutamate--homoc... 73 4e-12
>pir||B75138 tetrahydropteroyltriglutamate methyltransferase PAB2361 -
Pyrococcus abyssi (strain Orsay)
>gi|5458330|emb|CAB49819.1| (AJ248285)
TETRAHYDROPTEROYLTRIGLUTAMATE METHYLTRANSFERASE
[Pyrococcus abyssi]
Length = 313
Score = 622 bits (1588), Expect = e-177
Identities = 313/313 (100%), Positives = 313/313 (100%)
Query: 1 MYELIVPALIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVVT 60
MYELIVPALIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVVT
Sbjct: 1 MYELIVPALIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVVT 60
Query: 61 DGLYRWDDIFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELSLKENPIPEWISVAQD 120
DGLYRWDDIFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELSLKENPIPEWISVAQD
Sbjct: 61 DGLYRWDDIFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELSLKENPIPEWISVAQD 120
Query: 121 IKDEVYPEATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIKELDVSIVELQE 180
IKDEVYPEATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIKELDVSIVELQE
Sbjct: 121 IKDEVYPEATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIKELDVSIVELQE 180
Query: 181 PALAAELSKATREAVEHVSRDVAKSAIEELAKIKRLWVVTYFGTPQVVPRNVILNVDLVE 240
PALAAELSKATREAVEHVSRDVAKSAIEELAKIKRLWVVTYFGTPQVVPRNVILNVDLVE
Sbjct: 181 PALAAELSKATREAVEHVSRDVAKSAIEELAKIKRLWVVTYFGTPQVVPRNVILNVDLVE 240
Query: 241 GRIPEGLEGEVGLGIVNARETKMERADRLRDKLRKYVREFDVIYVTPNTLLDFLPESVAW 300
GRIPEGLEGEVGLGIVNARETKMERADRLRDKLRKYVREFDVIYVTPNTLLDFLPESVAW
Sbjct: 241 GRIPEGLEGEVGLGIVNARETKMERADRLRDKLRKYVREFDVIYVTPNTLLDFLPESVAW 300
Query: 301 RKLRLLGRLAGGE 313
RKLRLLGRLAGGE
Sbjct: 301 RKLRLLGRLAGGE 313
>pir||G71103 hypothetical protein PH1090 - Pyrococcus horikoshii
>gi|3257506|dbj|BAA30189.1| (AP000004) 309aa long
hypothetical protein [Pyrococcus horikoshii]
Length = 309
Score = 527 bits (1344), Expect = e-149
Identities = 250/308 (81%), Positives = 289/308 (93%)
Query: 4 LIVPALIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVVTDGL 63
+IVPALIGSLPRP+GLAKKIELYSIGRLSEEKLEEAYR YTR+AFENLKKA IKV+TDGL
Sbjct: 1 MIVPALIGSLPRPIGLAKKIELYSIGRLSEEKLEEAYRSYTRKAFENLKKARIKVITDGL 60
Query: 64 YRWDDIFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELSLKENPIPEWISVAQDIKD 123
YRWDDIFNP IRFI+GVE+NGLFKFYENNFFYRSPVV+GELSLKENP+P WIS+AQ+IK+
Sbjct: 61 YRWDDIFNPLIRFIDGVEVNGLFKFYENNFFYRSPVVRGELSLKENPLPGWISIAQEIKE 120
Query: 124 EVYPEATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIKELDVSIVELQEPAL 183
+VYPEA LKAVLPGPVTLAYHSINE+YK L+EL EAY V+ ELIKELDV I+ELQEP+L
Sbjct: 121 DVYPEAELKAVLPGPVTLAYHSINEYYKDLNELTEAYATVLRELIKELDVKIMELQEPSL 180
Query: 184 AAELSKATREAVEHVSRDVAKSAIEELAKIKRLWVVTYFGTPQVVPRNVILNVDLVEGRI 243
AAELSKATRE+ EHVS++VAK IE+LA++K+LWVVTYFGTPQVVP++VILN+DL+EG +
Sbjct: 181 AAELSKATRESEEHVSKEVAKKVIEDLARVKKLWVVTYFGTPQVVPKDVILNIDLIEGNV 240
Query: 244 PEGLEGEVGLGIVNARETKMERADRLRDKLRKYVREFDVIYVTPNTLLDFLPESVAWRKL 303
P+ ++GEVGLGIVNARETKMERADRL+DKLRKY+R+F+ +YVTPNTLLDFLPE VAW+KL
Sbjct: 241 PDEVKGEVGLGIVNARETKMERADRLKDKLRKYIRKFEKVYVTPNTLLDFLPERVAWKKL 300
Query: 304 RLLGRLAG 311
+LLGRL G
Sbjct: 301 KLLGRLGG 308
>gb|AAK40735.1| 5-methyltetrahydropteroyltriglutamate- homocysteine
methyltransferase (metE-1) [Sulfolobus solfataricus]
Length = 330
Score = 157 bits (394), Expect = 1e-37
Identities = 109/322 (33%), Positives = 178/322 (54%), Gaps = 26/322 (8%)
Query: 8 ALIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVVTDGLYRWD 67
AL+GS P+ L K I Y+ G++++EKLE+ + ++ FE ++ T+GL+RWD
Sbjct: 8 ALVGSFPKNPKLGKVISWYNTGKINKEKLEKHISENIKKLFELAGNIKLEYTTNGLFRWD 67
Query: 68 DIFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELSLK---ENPIPEWISVAQDIKDE 124
DI + FI G E L +F++NNF+YR P++K ++++K EN + + ++ IK+E
Sbjct: 68 DIVDVTFGFISGTEKGPLQRFFDNNFYYRQPIIKEKINVKKREENSFLQDLEFSRKIKEE 127
Query: 125 VYPEATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIKELD--VSIVELQEPA 182
V + LKAV+PGP+T S N +Y + EL+ Y V+ L +EL V +E+ EPA
Sbjct: 128 VSLPSKLKAVIPGPLTYYVLSDNRYYNSPIELMMDYASVVNSLSQELSSVVDAIEIHEPA 187
Query: 183 LAAELSKATREAVEHVSRDVAKSAIEELAKIKRLWVVTYFGTPQVVPRNVILN------- 235
+ K R+ +E + ++ KS ++ + K L +TYF + + + +
Sbjct: 188 IYTNNIK--RDILEKLP-EIYKSMLDNVKIEKHL--ITYFEINNLKRLDTLFSLPVDYFG 242
Query: 236 VDLVE-----GRIPEGLEG-EVGLGIVNARETKMERADRLRDKLRKYVRE--FDVIYVTP 287
+D++E GR+ +V LG++NAR TKMER + R+ DVI +
Sbjct: 243 IDVIENLKKLGRVYTYFSSRKVYLGMLNARNTKMERITTIIRVYNSVKRKGVSDVI-IGN 301
Query: 288 NTLLDFLPESVAWRKLRLLGRL 309
NTL DF+PE V +KL+LL +L
Sbjct: 302 NTLFDFIPEVVVVKKLKLLKKL 323
>gb|AAK40736.1| 5-methyltetrahydropteroyltriglutamate- homocysteine
methyltransferase (metE-2) [Sulfolobus solfataricus]
Length = 332
Score = 116 bits (289), Expect = 2e-25
Identities = 87/310 (28%), Positives = 149/310 (48%), Gaps = 15/310 (4%)
Query: 4 LIVPALIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVVTDGL 63
L+ +IGS PRP L + I L+ G++S E L+EA+ D F++ KAG+ V TDG
Sbjct: 6 LLPTTVIGSYPRPKWLRESIRLHKAGKMSNEDLQEAFNDAVIAVFQDHYKAGVDVPTDGE 65
Query: 64 YRWDDIFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELSLKENPIPEWISVAQDIKD 123
R D++ F I+G + G + + +YR P V ++ +E + + + A+ +
Sbjct: 66 VRRDEMVEFFAERIKGFKFYGPVRVW-GTAYYRKPSVVSKIEYREPMLVDEFTFAKSVS- 123
Query: 124 EVYPEATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIK---ELDVSIVELQE 180
Y + LK + GP T+A S NE+Y+ +LV I + IK E I+++ E
Sbjct: 124 --YTD-NLKITITGPYTIAEWSYNEYYRNKKDLVFDLAKAINQEIKNLVEAGAKIIQIDE 180
Query: 181 PALAAELSKAT--REAVEHVSRDVAKSAIEELAKIKRLWVVTYFGTPQVVPRNV---ILN 235
PAL + EAV + V + + +V YF +V N I N
Sbjct: 181 PALHTRKEDVSWGVEAVNEAVKGVNAKLVMHICYGDYSFVAPYFNEIKVDQINFALKIYN 240
Query: 236 VDLVEGRIPEGLEGEVGLGIVNARETKMERADRLRDKLRKYVREF--DVIYVTPNTLLDF 293
+E G + E+G G+V+ K+E ++ + + +RK + F + +++ P+ L
Sbjct: 241 YKPLELLKKYGFDKELGAGVVDVHNRKVETSEEVANDIRKILEYFPPEKVWINPDCGLKL 300
Query: 294 LPESVAWRKL 303
L +A++KL
Sbjct: 301 LSRKIAYQKL 310
>pir||D72509 hypothetical protein APE2050 - Aeropyrum pernix (strain K1)
>gi|5105748|dbj|BAA81060.1| (AP000063) 339aa long
hypothetical protein [Aeropyrum pernix]
Length = 339
Score = 103 bits (255), Expect = 2e-21
Identities = 97/329 (29%), Positives = 149/329 (44%), Gaps = 30/329 (9%)
Query: 1 MYELIVPA-LIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVV 59
M+ +I+ ++G PR + + + G +S +L + G V
Sbjct: 1 MFVMIIQGQVLGGYPRSRYVRRALRRLEQGEISAVELMGVLNQASSAVIGAQVSQGFPYV 60
Query: 60 TDGLYRWDDIFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELSLKENPI--PEWISV 117
DG+ W DIF PF V GL ++++NNFFYR P+ GE +P+ P +S
Sbjct: 61 VDGMLDWHDIFRPFAEAWRNVTPTGLLRYFDNNFFYRIPLFTGEPE-PTDPVLAPRVLSY 119
Query: 118 AQDIKDEVYPEATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIK---ELDVS 174
+Q V A LK V+PGPVT S NE + +EL EA +++A+ ++ +
Sbjct: 120 SQ-----VAEPAKLKVVVPGPVTFTLMSRNESGISDEELAEAIANLLADEVEKAVKAGAG 174
Query: 175 IVELQEPALAAELSKATREAVEHVSRDVAKSAIEELAKIKRLWVVTYFGTPQVV-----P 229
V++ EP L+ KATR+ + A + L V Y P+V
Sbjct: 175 FVQVDEPILSE--PKATRDHALLAAELAGVIASRSGSAETMLSVYFYPPKPEVYEALLEA 232
Query: 230 RNVILNVDLVEGRIPEGLE-------GE-VGLGIVNARETKMERADRLRDKLRKYVREF- 280
R L VDLV +GLE GE + LG+++AR E +++ +R+ V
Sbjct: 233 RVDYLMVDLVAAG-SKGLEVITGKGAGEGLALGVIDARSIYSESLEKVEAPVRRIVESVK 291
Query: 281 -DVIYVTPNTLLDFLPESVAWRKLRLLGR 308
+ I +T +T LD +P A RK LLGR
Sbjct: 292 PEKIILTTSTWLDLIPYRYALRKTALLGR 320
>pir||C75138 probable 5-methyltetrahydropteroyltriglutamate- homocysteine
methyltransferase PAB0608 - Pyrococcus abyssi (strain
Orsay) >gi|5458331|emb|CAB49820.1| (AJ248285) related
5-methyltetrahydropteroyltriglutamate-homocysteine
methyltransferase, putative [Pyrococcus abyssi]
Length = 338
Score = 79.6 bits (193), Expect = 4e-14
Identities = 74/311 (23%), Positives = 144/311 (45%), Gaps = 18/311 (5%)
Query: 4 LIVPALIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVVTDGL 63
++ ++IGS P+P L + +L +G++ EE +EA +D + ++AG+ + DG
Sbjct: 5 ILPTSVIGSYPKPRWLLRMYKLRELGKIPEEDFKEAVKDASVAVLREHERAGVDIPWDGE 64
Query: 64 YRWDDIFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELSLKEN-PIPEWISVAQDIK 122
++ F I G + G + + N +F ++ V +L KE + E++ V ++
Sbjct: 65 MWRSEMTEHFTAKISGFKFYGPVRVWGNAYFNKAAAV-DKLEYKEPLVLEEFLWVRENTT 123
Query: 123 DEVYPEATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIKELD---VSIVELQ 179
E+ +K + GP T+A S NE+Y + V +I + +K L+ + ++L
Sbjct: 124 REI-----VKVPITGPYTIAEWSFNEYYPDKESFVMDLAKIINKELKTLEEHGATYIQLD 178
Query: 180 EPALAAELSKATREAVEHVSRDVAKSAIE---ELAKIKRLWVVTYFGTPQVVPRNV-ILN 235
EPA+ + AVE ++R V I+ + + YF +V + N
Sbjct: 179 EPAMLNHPDEVPL-AVEAINRAVKGIKIKVGLHVCYSNYYLLADYFDDIRVTQFALEFAN 237
Query: 236 VDLVEGRIPEGLEG-EVGLGIVNARETKMERADRLRDKLRKYVR--EFDVIYVTPNTLLD 292
+ + L G E+G G+V+ ++E D + ++K E + +Y+ P+ L
Sbjct: 238 RQFRDMDFLKKLSGKELGFGVVDVHNPRIETVDEIVRAIKKAFNYLEPEWLYINPDCGLK 297
Query: 293 FLPESVAWRKL 303
L +A++KL
Sbjct: 298 LLDRRIAYQKL 308
>pir||B72509 probable 5-methltetrahydropteroyltriglutamate--homocysteinemethyltr
ansferase APE2048 - Aeropyrum pernix (strain K1)
>gi|5105746|dbj|BAA81058.1| (AP000063) 332aa long
hypothetical
5-methltetrahydropteroyltriglutamate--
homocysteinemethyltr ansferase [Aeropyrum pernix]
Length = 332
Score = 73.0 bits (176), Expect = 4e-12
Identities = 69/320 (21%), Positives = 142/320 (43%), Gaps = 39/320 (12%)
Query: 9 LIGSLPRPVGLAKKIELYSIGRLSEEKLEEAYRDYTRRAFENLKKAGIKVVTDGLYRWDD 68
++GS P+ ++ I+ G +SEE+ R+ R E+ AG+ +++DG +D
Sbjct: 15 VVGSYPKIGPASEAIKKRRAGEISEEEFHRIARESIRMVVEDYIWAGVDIISDGEQTRED 74
Query: 69 IFNPFIRFIEGVELNGLFKFYENNFFYRSPVVKGELS-LKENPIPEWISVAQDIKDEVYP 127
+ F +EG + ++N +F R P+V G + ++ I +W + +
Sbjct: 75 MVVYFAERLEGYREGDWVRVFDNVYF-RKPIVAGRVRWVRPMIIEDW-----EYARSISA 128
Query: 128 EATLKAVLPGPVTLAYHSINEHYKTLDELVEAYKDVIAELIKELDVSI------VELQEP 181
+K ++ GP T+ S + HY EL+ D+ + +E+D ++ +++ EP
Sbjct: 129 GRPVKFIITGPYTMLEWSFDLHYGDRRELI---FDLARAIRREIDEALARGAEYIQVDEP 185
Query: 182 ALAAELSKATREAVEHVSRDVAKSAIEELAKIKRLWVVTYFGTPQVVPRNVILNVDLVEG 241
AL+ K E ++ A I + KR+ + Y +++P + VD +
Sbjct: 186 ALSTRPFKEEAELLKE-----ALDIIFKGVGAKRIVHICYGELERILPYILDYPVDQFDL 240
Query: 242 RIPE------------GLEGEVGLGIVNARETKMERADRLRDKLRKYVREFDV-----IY 284
+ G + E+G G+++ ++E +++ + + ++ D+ +Y
Sbjct: 241 EMKNSNFRLLPYLKEYGYDKEIGYGVIDVHSFQVESVGEVKEAIDRLMK-MDIVGPEKVY 299
Query: 285 VTPNTLLDFLPESVAWRKLR 304
V P+ L LP VA KL+
Sbjct: 300 VDPDCGLKRLPRDVARAKLK 319
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.140 0.398
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 115857032
Number of Sequences: 2977
Number of extensions: 4926473
Number of successful extensions: 15912
Number of sequences better than 1.0e-10: 7
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 15898
Number of HSP's gapped (non-prelim): 7
length of query: 313
length of database: 189,106,746
effective HSP length: 56
effective length of query: 257
effective length of database: 155,591,474
effective search space: 39987008818
effective search space used: 39987008818
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 165 (68.7 bits)