BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2365 (phoX) DE:phosphate ABC transporter, periplasmic
phosphate-binding protein (phoX)
(383 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75081 phosphate abc transporter, periplasmic phosphate-bin... 779 0.0
pir||F64426 phosphate-binding protein - Methanococcus jannaschii... 570 e-161
pir||H72756 probable phosphate-binding periplasmic protein APE00... 276 3e-73
gb|AAK40810.1| Phosphate binding periplasmic protein precursor (... 244 1e-63
pir||C70473 phosphate-binding periplasmic protein - Aquifex aeol... 238 1e-61
pir||S74423 phosphate-binding periplasmic protein pstS-1 - Synec... 214 1e-54
sp|P45192|PSTS_HAEIN PHOSPHATE-BINDING PERIPLASMIC PROTEIN PRECU... 204 2e-51
emb|CAA57231.1| (X81478) phosphate binding protein [Xanthomonas ... 194 2e-48
pir||H75611 phosphate ABC transporter, periplasmic phosphate-bin... 188 8e-47
dbj|BAA22861.1| (D89963) phosphate-binding protein [Enterobacter... 188 1e-46
sp|P06128|PSTS_ECOLI PHOSPHATE-BINDING PERIPLASMIC PROTEIN PRECU... 185 5e-46
gb|AAA62079.1| (L10328) periplasmic phosphate-binding protein [E... 185 5e-46
pdb|1IXH| Phosphate-Binding Protein (Pbp) Complexed With Phosp... 185 5e-46
pdb|1A55|A Chain A, Phosphate-Binding Protein Mutant A197c >gi|3... 185 9e-46
pdb|1A40| Phosphate-Binding Protein With Ala 197 Replaced With... 184 2e-45
pdb|1QUK| Phosphate-Binding Protein Mutant With Asp 137 Replac... 183 2e-45
pdb|1IXI| Phosphate-Binding Protein Mutant With Asp 56 Replace... 183 2e-45
pdb|1IXG| Phosphate-Binding Protein Mutant With Thr 141 Replac... 183 3e-45
pdb|1QUL| Phosphate-Binding Protein Mutant With Asp 137 Replac... 183 4e-45
pdb|1QUI| Phosphate-Binding Protein Mutant With Asp 137 Replac... 183 4e-45
pir||G82595 ABC transporter phosphate binding protein XF2141 [im... 182 8e-45
pir||I64120 phosphate-binding protein, phosphate-repressible - H... 174 1e-42
emb|CAB41939.1| (AJ132617) Putative PstS protein [Burkholderia sp.] 162 8e-39
pir||S74876 phosphate-binding periplasmic protein pstS-2 - Synec... 153 4e-36
sp|P15712|PAB_MYCTU PROTEIN ANTIGEN B PRECURSOR (PAB) >gi|98820|... 151 1e-35
emb|CAA64783.1| (X95538) PstS subunit of ABC transporter [Mycoba... 142 7e-33
gb|AAF74819.1|AF137360_1 (AF137360) periplasmic phosphate permea... 140 3e-32
emb|CAB88475.1| (AL353816) phosphate-binding protein precursor [... 139 4e-32
gb|AAA63076.1| (U15184) phosphate-repressible, periplasmic bindi... 138 1e-31
pir||S54862 PstS-3 protein precursor - Mycobacterium tuberculosi... 133 3e-30
gb|AAA62993.1| (U15182) match to phosphate-repressible, periplas... 132 5e-30
emb|CAA64784.1| (X95538) PstS subunit of ABC transporter [Mycoba... 132 8e-30
gb|AAC45381.1| (U75514) PstS [Prochlorococcus marinus] 132 8e-30
pir||H70583 phosphate-binding protein S-3 precursor, periplasmic... 132 8e-30
pir||S39852 phosphate-binding protein, periplasmic - Synechococc... 125 8e-28
pir||D70584 phosphate uptake system component pstS-2 precursor -... 125 1e-27
pir||S54861 PstS-2 protein precursor - Mycobacterium tuberculosis 124 2e-27
gb|AAB70458.1| (U70621) phosphate-binding protein precursor [Pec... 116 4e-25
pir||C72276 phosphate ABC transporter, periplasmic phosphate-bin... 84 2e-15
>pir||D75081 phosphate abc transporter, periplasmic phosphate-binding protein
(phox) PAB2365 - Pyrococcus abyssi (strain Orsay)
>gi|5458465|emb|CAB49953.1| (AJ248286) phosphate ABC
transporter, periplasmic phosphate-binding protein
(phoX) [Pyrococcus abyssi]
Length = 383
Score = 779 bits (1991), Expect = 0.0
Identities = 383/383 (100%), Positives = 383/383 (100%)
Query: 1 MVSSMRWMALLLMGLVGVGLLISGCIGEETPTHQPKVNSRSKAKNPEIIVIRTSGATFPQ 60
MVSSMRWMALLLMGLVGVGLLISGCIGEETPTHQPKVNSRSKAKNPEIIVIRTSGATFPQ
Sbjct: 1 MVSSMRWMALLLMGLVGVGLLISGCIGEETPTHQPKVNSRSKAKNPEIIVIRTSGATFPQ 60
Query: 61 YQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGD 120
YQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGD
Sbjct: 61 YQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGD 120
Query: 121 QPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHKK 180
QPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHKK
Sbjct: 121 QPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHKK 180
Query: 181 IIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVAAL 240
IIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVAAL
Sbjct: 181 IIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVAAL 240
Query: 241 KQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDPTEGYKE 300
KQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDPTEGYKE
Sbjct: 241 KQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDPTEGYKE 300
Query: 301 DLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENLAP 360
DLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENLAP
Sbjct: 301 DLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENLAP 360
Query: 361 GYVGLPKEVAEIGLKAVEMIEER 383
GYVGLPKEVAEIGLKAVEMIEER
Sbjct: 361 GYVGLPKEVAEIGLKAVEMIEER 383
>pir||F64426 phosphate-binding protein - Methanococcus jannaschii
>gi|1591675|gb|AAB99019.1| (U67544) periplasmic
phosphate-binding protein (pstS) [Methanococcus
jannaschii]
Length = 389
Score = 570 bits (1453), Expect = e-161
Identities = 278/380 (73%), Positives = 324/380 (85%), Gaps = 12/380 (3%)
Query: 8 MALLL-MGLVGVGLLISGCIGEETPTHQPKVNSRSKAKNPEIIVIRTSGATFPQYQIQKW 66
+AL+L + L+ + I+GC+G QP N + P I+IRT+GATFP+YQIQKW
Sbjct: 17 LALILGLCLIVPVISIAGCVGGGNS--QPSNNEK-----PSTIIIRTTGATFPKYQIQKW 69
Query: 67 IEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGDQPLQFP 126
IE YQK +P+VKIEYEGGGSGYGQEAF KGLTDIGRTDPPVKES WKKFL TGDQPLQFP
Sbjct: 70 IEDYQKTHPNVKIEYEGGGSGYGQEAFAKGLTDIGRTDPPVKESMWKKFLSTGDQPLQFP 129
Query: 127 EIVGAVVVVYNVPEVNE--LKLSREVLAKIFLGEIEYWDDPAIKRLNPN--EKLPHKKII 182
EIVGAVVV YN+PE+ + LKLSR+VLA IFLG+IEYWDD IK++NP +KLPH+KII
Sbjct: 130 EIVGAVVVTYNIPEIGDKTLKLSRDVLADIFLGKIEYWDDERIKKINPEIADKLPHEKII 189
Query: 183 VIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVAALKQ 242
V+HRSDASGTTAIFTTYLSLISKEWAEKVG GK V+WP D +GRG+ KGNPGVVA +K
Sbjct: 190 VVHRSDASGTTAIFTTYLSLISKEWAEKVGAGKTVNWPTDNIGRGVAGKGNPGVVAIVKS 249
Query: 243 TKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDPTEGYKEDL 302
T Y++AYTELS+AIE+ L VAALENK GKFVKP +ETIKAAV+ VKA IP+PTEGYKEDL
Sbjct: 250 TPYTVAYTELSYAIEQKLPVAALENKNGKFVKPTDETIKAAVSAVKASIPNPTEGYKEDL 309
Query: 303 KQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENLAPGY 362
KQ+LDAPG+N+YPIVAFTH+LVW+NK GKHY+PEKAKAIKDFL W+LTEGQKPE+LAPGY
Sbjct: 310 KQMLDAPGDNAYPIVAFTHLLVWENKNGKHYSPEKAKAIKDFLTWVLTEGQKPEHLAPGY 369
Query: 363 VGLPKEVAEIGLKAVEMIEE 382
VGLP++VA+IGL AV MI+E
Sbjct: 370 VGLPEDVAKIGLNAVNMIKE 389
>pir||H72756 probable phosphate-binding periplasmic protein APE0045 - Aeropyrum
pernix (strain K1) >gi|5103433|dbj|BAA78954.1|
(AP000058) 373aa long hypothetical phosphate-binding
periplasmic protein [Aeropyrum pernix]
Length = 373
Score = 276 bits (698), Expect = 3e-73
Identities = 149/376 (39%), Positives = 221/376 (58%), Gaps = 22/376 (5%)
Query: 10 LLLMGLVGVGL-LISGCIGEETPTHQPKVNSRSKAKNPEIIVIRTSGATFPQYQIQKWIE 68
L ++ L+G+GL L SG +G E S P +V++ G+TF QIQ W E
Sbjct: 11 LAVVVLLGIGLALASGWLGGEQ-------GESSGGSGPAQVVLQGEGSTFIYPQIQAWSE 63
Query: 69 KYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGDQPLQFPEI 128
+ +K P + I Y GSG GQ AF G+ D +D P+ W +Q P +
Sbjct: 64 EIKKTYPWITINYNPTGSGAGQSAFKDGVVDFAGSDTPLPREVWSAMQ---GNVIQIPVV 120
Query: 129 VGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHKKIIVIHRSD 188
+G + +VYN+P + +LKL + +A I+ GEIEYWDDP I LNP LPH+KI+ +HRSD
Sbjct: 121 IGMLAIVYNIPGLEDLKLDAKTIALIYKGEIEYWDDPRIASLNPGASLPHEKIVAVHRSD 180
Query: 189 ASGTTAIFTTYLSLISKE-WAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVAALKQTKYSI 247
+SGTT +FT +L + + W E + VGK VDWPVD GRG+G KGN GV+ +K T YSI
Sbjct: 181 SSGTTHVFTIFLHKGAPDVWTEDL-VGKSVDWPVDATGRGVGGKGNQGVMEVVKNTPYSI 239
Query: 248 AYTELSFAIE--ENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPD-PTEGYKEDLKQ 304
Y E ++ ++ E + VA + N+ F+KP+ E +AA + +PD P + +
Sbjct: 240 GYVEYAYVVKAGEGVNVALVANRDDVFLKPSPEGAQAAASGAVQNLPDSPDDDWSTGYDA 299
Query: 305 LLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENLAPGYVG 364
++ APG++SYPI +++ +L ++ + +KA+AIK F+ WI TEGQ + GY+
Sbjct: 300 IIYAPGKDSYPITSWSFLLFYKQYNDR----DKAEAIKKFIEWINTEGQ--TKIIEGYIP 353
Query: 365 LPKEVAEIGLKAVEMI 380
+P E+ +I +KAVEMI
Sbjct: 354 IPDEIRQINMKAVEMI 369
>gb|AAK40810.1| Phosphate binding periplasmic protein precursor (pstS) [Sulfolobus
solfataricus]
Length = 405
Score = 244 bits (616), Expect = 1e-63
Identities = 142/387 (36%), Positives = 218/387 (55%), Gaps = 20/387 (5%)
Query: 8 MALLLMGLVGVGLLISGCIGEETPTHQPKVN---------SRSKAKNPEIIVIRTSGATF 58
+ ++++ + G+ +I+ G T T + S++ A + I G+TF
Sbjct: 13 LIIVVIAVAGIFFVINSQGGHNTTTTSTSSSFSTSSQASSSQTTASSQISASIIAGGSTF 72
Query: 59 PQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQT 118
+Q WI+ +Q +++ Y GSG G FL+G DIG TD P + +++ Q
Sbjct: 73 VNPLMQVWIKDFQSNYNGIQVTYSAVGSGAGVNNFLQGAYDIGATDVPPPSNLYQQLTQK 132
Query: 119 GDQPLQFPEIVGAVVVVYNVPEVN-ELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLP 177
+ L P++VGAV ++YN+P + L L+ +VLAKI+LG+I+YWDDPAIK LNP+
Sbjct: 133 YGEVLTIPDVVGAVDIIYNIPSFSGTLNLTADVLAKIYLGQIQYWDDPAIKALNPHFNFT 192
Query: 178 HKKIIVIHRSDASGTTAIFTTYLSLISKEW-AEKVGVGKVVDWPVDKVGRGIGAKGNPGV 236
H+KII +HRSD SGTT IFT +L S+ W + V G V+WPVDK+G G+G KG+ GV
Sbjct: 193 HQKIIAVHRSDGSGTTFIFTYWLYTSSQSWRSSNVSYGYTVNWPVDKLGNGLGGKGSDGV 252
Query: 237 VAALKQTKYSIAYTELSFAIEENLKVAALEN-KAGKFVKPNEETIKAAV--ANVKAFIPD 293
A + Q YSI Y E +AI +NL AA+ N G +V P + +I+ A+ AN+ +
Sbjct: 253 TAYVSQNPYSIGYVEAQYAIAKNLTPAAVLNPSTGDYVLPTQASIETAIQNANLSSLPSS 312
Query: 294 PTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQ 353
T + L L+ N+YPIV F+ +++ N K KA+AI FL++I T GQ
Sbjct: 313 LTRDLSQYLSVFLNVKAHNAYPIVTFSWLVIKVNYTDK----SKAEAIYLFLKYIATTGQ 368
Query: 354 KPENLAPGYVGLPKEVAEIGLKAVEMI 380
L GY+ LP + ++ L+ +++I
Sbjct: 369 --TELPSGYIELPSNIQQLILQNLKLI 393
>pir||C70473 phosphate-binding periplasmic protein - Aquifex aeolicus
>gi|2984256|gb|AAC07783.1| (AE000768) phosphate-binding
periplasmic protein [Aquifex aeolicus]
Length = 337
Score = 238 bits (600), Expect = 1e-61
Identities = 140/320 (43%), Positives = 178/320 (54%), Gaps = 22/320 (6%)
Query: 50 VIRTSGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKE 109
VI +GATFP KW + Y K +K+ Y+ GSG G + D G +D P+K
Sbjct: 26 VINGAGATFPAPLYWKWADAYYKATG-IKVNYQSIGSGGGVRQIVNRTVDFGASDAPLKP 84
Query: 110 STWKKFLQTGDQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKR 169
KK QFP ++G V V YN+ V LKLS + IFLG+I+YWDDP IK+
Sbjct: 85 EETKKH-----NLAQFPTVIGGVTVAYNLRGVENLKLSTRAVCDIFLGKIKYWDDPVIKK 139
Query: 170 LNPNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIG 229
NP KLPHK+I V+ RSD SGTT IFT YLS EW EKVG GK V WP GIG
Sbjct: 140 DNPGVKLPHKRITVVRRSDGSGTTWIFTNYLSKACPEWKEKVGYGKAVRWPT-----GIG 194
Query: 230 AKGNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKA 289
AKGN GV +++ K +I Y E ++AI+ + VAA+EN+ GKFVKP+ ET +AA AN K
Sbjct: 195 AKGNEGVANYIRRIKGAIGYVEYAYAIQNKIPVAAVENRDGKFVKPSIETFQAAAANAKW 254
Query: 290 FIPDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWIL 349
DP + + E L PGE +YPI T IL+ +++ K E K F W
Sbjct: 255 ---DPKKDFYEVLTW---QPGEKAYPIAGATFILLAKDEDRKEINKEVVK----FFDWAY 304
Query: 350 TEGQKPENLAPGYVGLPKEV 369
G K + YV LP+ V
Sbjct: 305 KNGDKMA-IELHYVPLPENV 323
>pir||S74423 phosphate-binding periplasmic protein pstS-1 - Synechocystis sp.
(strain PCC 6803) >gi|1001197|dbj|BAA10341.1| (D64001)
phosphate-binding periplasmic protein precursor (PBP)
[Synechocystis sp.]
Length = 383
Score = 214 bits (539), Expect = 1e-54
Identities = 125/330 (37%), Positives = 188/330 (56%), Gaps = 23/330 (6%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GA+FP Q W + P++++ Y+ GSG G E F+ D G +D + + +
Sbjct: 65 AGASFPAPLYQGWFVALNQAVPNLEVNYQSVGSGAGVEQFMSKTVDFGASDVAMDD---E 121
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPN 173
+ + + + P G++V+ YN+P V LKLS+E LA I LG I W+DP + NP+
Sbjct: 122 EIAKVNGEVVMLPMTAGSIVMAYNLPGVEGLKLSQEALAGIMLGNITKWNDPKLVADNPD 181
Query: 174 EKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGN 233
LP + I V+HRSD SGTTA+FT L+ +S E+ E +G GK V+WP K G+ IG KGN
Sbjct: 182 LTLPDRPITVVHRSDGSGTTAVFTMNLAAMSPEFKETIGDGKTVEWPTSK-GKFIGGKGN 240
Query: 234 PGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPD 293
GV A ++Q + +I Y E +A NL +A+L+NK G+FV P +E A +A V+
Sbjct: 241 EGVTAGIQQNEGAIGYVEYGYATNNNLTMASLQNKDGQFVVPTDENASATLAAVE----- 295
Query: 294 PTEGYKEDLKQLLDAP-GENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEG 352
E+L++ + P G +SYPIV +T +L++ ++ EKAK I+ + + L EG
Sbjct: 296 ----LPENLREFITNPAGADSYPIVTYTWMLLYP----QYADAEKAKGIEAMVEFGLNEG 347
Query: 353 QKPENLAP--GYVGLPKEVAEIGLKAVEMI 380
Q +AP GYV LP+ V E A + I
Sbjct: 348 Q---TMAPTLGYVPLPQNVREKVAAAADKI 374
>sp|P45192|PSTS_HAEIN PHOSPHATE-BINDING PERIPLASMIC PROTEIN PRECURSOR (PBP)
Length = 334
Score = 204 bits (513), Expect = 2e-51
Identities = 127/324 (39%), Positives = 181/324 (55%), Gaps = 30/324 (9%)
Query: 51 IRTSGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKES 110
I +GA+FP KW Y+K + K+ Y+ GSG GQ+ + D G +D P+K
Sbjct: 22 ITGAGASFPYPIYAKWASLYEKETGN-KVNYQSIGSGGGQQQIIAKTVDFGASDDPMKSE 80
Query: 111 TWKKFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIK 168
++ Q +QFP ++G +V V N+PE+ +LKLS ++LA+IFLG+I+ W+DP +
Sbjct: 81 LLQQH-----QLVQFPAVIGGIVPVVNLPEIKPGKLKLSGKLLAEIFLGKIKKWNDPDLV 135
Query: 169 RLNPNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGI 228
LNP LP+K IIVIHRSD SGTT FT YLS IS +W +VG GK V W G
Sbjct: 136 ALNPTLPLPNKNIIVIHRSDGSGTTFGFTNYLSKISNDWKNQVGEGKSVKWLT-----GQ 190
Query: 229 GAKGNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVK 288
G KGN GV + ++Q KYSI Y E ++A + L +L+N+AG+FV+P+ E+ AA ++ K
Sbjct: 191 GGKGNEGVASYVRQMKYSIGYVEYAYAKQNQLAWISLQNQAGQFVQPSNESFMAAASHAK 250
Query: 289 AFIPDPTEGYKEDLKQ---LLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFL 345
+ E L + GE S+PI A + IL+ NK + PE K + F
Sbjct: 251 ---------WHEKAGMGVILTNETGEKSWPITAASFILL--NKYSDN--PETTKNVLAFF 297
Query: 346 RWILTEGQKPENLAPGYVGLPKEV 369
W + GQ YV +P +V
Sbjct: 298 DWAFSRGQDAAT-ELDYVPIPADV 320
>emb|CAA57231.1| (X81478) phosphate binding protein [Xanthomonas oryzae]
Length = 339
Score = 194 bits (487), Expect = 2e-48
Identities = 124/316 (39%), Positives = 167/316 (52%), Gaps = 26/316 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+G +F + KW Y ++ Y+ GSG G D G +D P+K +
Sbjct: 30 AGRSFIYPVMSKWSADYNAATKK-QVNYQSIGSGGGIAQIKAASVDFGSSDAPLKP---E 85
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEV--NELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
+ G QFP ++G VV V NVP V LKL + L IFLG++ W+DPAI LN
Sbjct: 86 ELAAAG--LAQFPSVIGGVVPVVNVPGVAAGALKLDGKTLGDIFLGKVSTWNDPAIAALN 143
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP KI V+HRSD SGT+ FT YLS ++ +W KVG G V WP GIG K
Sbjct: 144 PGVKLPEGKITVVHRSDGSGTSFNFTNYLSKVNPDWKSKVGEGTAVQWPT-----GIGGK 198
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN GV A +KQ K I Y ELS+A++ + A++N AGKFV+P++ET AA AN +
Sbjct: 199 GNEGVAAYVKQIKGGIGYVELSYALQNKMAYTAMKNAAGKFVQPSDETF-AAAANSADW- 256
Query: 292 PDPTEGYKEDLKQLL-DAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILT 350
G +D ++ +A G+N++PI A ILV + K P K DF RW+ +
Sbjct: 257 -----GSSKDFYLVMTNAAGDNAWPITATNFILVQK----KPKNPTGLKNTLDFFRWVYS 307
Query: 351 EGQKPENLAPGYVGLP 366
+G YV LP
Sbjct: 308 KGDAQAKQL-DYVPLP 322
>pir||H75611 phosphate ABC transporter, periplasmic phosphate-binding protein -
Deinococcus radiodurans (strain R1)
>gi|6460501|gb|AAF12207.1|AE001862_33 (AE001862)
phosphate ABC transporter, periplasmic phosphate-binding
protein [Deinococcus radiodurans]
Length = 403
Score = 188 bits (473), Expect = 8e-47
Identities = 118/324 (36%), Positives = 170/324 (52%), Gaps = 28/324 (8%)
Query: 51 IRTSGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKES 110
I +GA+FP K +Y+ N + Y+ GSG GQ+ L+ D +D P+ ++
Sbjct: 87 ITGAGASFPYPLYSKMFSEYKASN----VNYQSVGSGSGQKQILERTVDFAGSDNPMTDA 142
Query: 111 TWKKFLQTGDQP---LQFPEIVGAVVVVYNVPEVNE-LKLSREVLAKIFLGEIEYWDDPA 166
Q G P L P +GAVV YN+P V + L LA I+LG+I+ W DPA
Sbjct: 143 ------QLGSAPGTLLHVPTAIGAVVPAYNLPGVTKPLNFDGPTLANIYLGKIKTWGDPA 196
Query: 167 IKRLNPNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGR 226
I +LNP +P I V RSD SGTT +F+ YLS +S EW KVG G + WPV
Sbjct: 197 IAKLNPGVTIPPLPITVARRSDGSGTTFVFSDYLSKVSGEWKSKVGAGNSLQWPV----- 251
Query: 227 GIGAKGNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVAN 286
G GAKGN GV +K T +I Y EL +A + L A++N+AGKF+ + A
Sbjct: 252 GTGAKGNDGVAGVVKGTPGAIGYVELVYAKQNKLSFGAVKNRAGKFILADNGPASNAALG 311
Query: 287 VKAFIPDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLR 346
V IP T L ++ +YPI +FT+++ ++++ + T +AKA+K+ L
Sbjct: 312 V--VIPADTR------VSLTNSANAGAYPIASFTYLIFYKDQKYGNRTEAQAKALKNLLT 363
Query: 347 WILTEGQKPENLAPGYVGLPKEVA 370
+++T GQ+ N Y LP VA
Sbjct: 364 YVVTSGQQ-YNEGLDYAKLPSNVA 386
>dbj|BAA22861.1| (D89963) phosphate-binding protein [Enterobacter cloacae]
Length = 346
Score = 188 bits (472), Expect = 1e-46
Identities = 116/320 (36%), Positives = 167/320 (51%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + D G +D P+ +
Sbjct: 32 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQITANTVDFGASDAPLSDD--- 87
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
+ Q G QFP ++G VV+ N+P V EL L + L I+LG+I+ WDD AI +LN
Sbjct: 88 RLAQEG--LFQFPTVIGGVVLAINLPGVKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 145
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+D SGT+ +FT+YL+ +++EW KVG G V+WP G+G K
Sbjct: 146 PGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEEWKSKVGSGSTVNWPT-----GLGGK 200
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ SI Y E ++A + NL L + GK V P EE A
Sbjct: 201 GNDGIAAFVQRLPGSIGYVEYAYAKQNNLSYTKLVSADGKPVSPTEENFANAAKGA---- 256
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE ++PI + T ILV + + PE+ + F W
Sbjct: 257 -DWSKSFAQD---LTNQKGEGAWPITSTTFILVHK----EQKKPEQGAEVLKFFDWAYKN 308
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N A Y LP V E
Sbjct: 309 GGKQAN-ALDYATLPDNVVE 327
>sp|P06128|PSTS_ECOLI PHOSPHATE-BINDING PERIPLASMIC PROTEIN PRECURSOR (PBP)
>gi|72445|pir||BYECPR phosphate-repressible
phosphate-binding protein precursor [validated] -
Escherichia coli >gi|147256|gb|AAA24378.1| (K01992)
phosphate-binding protein [Escherichia coli]
>gi|2367271|gb|AAC76751.1| (AE000449) high-affinity
phosphate-specific transport system; periplasmic
phosphate-binding protein [Escherichia coli K12]
Length = 346
Score = 185 bits (466), Expect = 5e-46
Identities = 112/320 (35%), Positives = 169/320 (52%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ + +
Sbjct: 32 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASDAPLSD---E 87
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 88 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 145
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+D SGT+ +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 146 PGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 200
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E ++A + NL L + GK V P EE A
Sbjct: 201 GNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 256
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 257 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQGTEVLKFFDWAYKT 308
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 309 GAKQAN-DLDYASLPDSVVE 327
>gb|AAA62079.1| (L10328) periplasmic phosphate-binding protein [Escherichia coli]
Length = 346
Score = 185 bits (466), Expect = 5e-46
Identities = 112/320 (35%), Positives = 169/320 (52%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ + +
Sbjct: 32 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASDAPLSD---E 87
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 88 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 145
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+D SGT+ +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 146 PGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 200
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E ++A + NL L + GK V P EE A
Sbjct: 201 GNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 256
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 257 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQXTEVLKFFDWAYKT 308
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 309 GAKQAN-DLDYASLPDSVVE 327
>pdb|1IXH| Phosphate-Binding Protein (Pbp) Complexed With Phosphate
>gi|2094889|pdb|2ABH| Phosphate-Binding Protein
(Re-Refined)
Length = 321
Score = 185 bits (466), Expect = 5e-46
Identities = 112/320 (35%), Positives = 169/320 (52%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ + +
Sbjct: 7 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASDAPLSD---E 62
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 63 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 120
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+D SGT+ +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 121 PGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 175
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E ++A + NL L + GK V P EE A
Sbjct: 176 GNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 231
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 232 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQGTEVLKFFDWAYKT 283
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 284 GAKQAN-DLDYASLPDSVVE 302
>pdb|1A55|A Chain A, Phosphate-Binding Protein Mutant A197c
>gi|3892021|pdb|1A54|A Chain A, Phosphate-Binding
Protein Mutant A197c Labelled With A Coumarin
Fluorophore And Bound To Dihydrogenphosphate Ion
Length = 321
Score = 185 bits (464), Expect = 9e-46
Identities = 112/320 (35%), Positives = 168/320 (52%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ + +
Sbjct: 7 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASDAPLSD---E 62
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 63 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 120
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+D SGT+ +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 121 PGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 175
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E +A + NL L + GK V P EE A
Sbjct: 176 GNDGIAAFVQRLPGAIGYVEYCYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 231
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 232 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQGTEVLKFFDWAYKT 283
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 284 GAKQAN-DLDYASLPDSVVE 302
>pdb|1A40| Phosphate-Binding Protein With Ala 197 Replaced With Trp
Length = 321
Score = 184 bits (462), Expect = 2e-45
Identities = 112/320 (35%), Positives = 168/320 (52%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ + +
Sbjct: 7 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASDAPLSD---E 62
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 63 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 120
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+D SGT+ +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 121 PGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 175
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E +A + NL L + GK V P EE A
Sbjct: 176 GNDGIAAFVQRLPGAIGYVEYWYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 231
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 232 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQGTEVLKFFDWAYKT 283
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 284 GAKQAN-DLDYASLPDSVVE 302
>pdb|1QUK| Phosphate-Binding Protein Mutant With Asp 137 Replaced By Asn
Complex With Phosphate
Length = 321
Score = 183 bits (461), Expect = 2e-45
Identities = 111/320 (34%), Positives = 169/320 (52%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ + +
Sbjct: 7 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASDAPLSD---E 62
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 63 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 120
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R++ SGT+ +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 121 PGLKLPSQNIAVVRRANGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 175
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E ++A + NL L + GK V P EE A
Sbjct: 176 GNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 231
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 232 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQGTEVLKFFDWAYKT 283
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 284 GAKQAN-DLDYASLPDSVVE 302
>pdb|1IXI| Phosphate-Binding Protein Mutant With Asp 56 Replaced By Asn
Complex With Monobasic Phosphate Ion
Length = 321
Score = 183 bits (461), Expect = 2e-45
Identities = 111/320 (34%), Positives = 169/320 (52%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G ++ P+ + +
Sbjct: 7 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASNAPLSD---E 62
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 63 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 120
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+D SGT+ +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 121 PGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 175
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E ++A + NL L + GK V P EE A
Sbjct: 176 GNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 231
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 232 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQGTEVLKFFDWAYKT 283
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 284 GAKQAN-DLDYASLPDSVVE 302
>pdb|1IXG| Phosphate-Binding Protein Mutant With Thr 141 Replaced By Asp
(T141d), Complexed With Phospate >gi|640347|pdb|1PBP|
Title: Phosphate-Binding Protein (Mutant With Thr 141
Replaced By Asp) Complexed With Monobasic Phosphate Ion.
Molecule: Phosphate-Binding Protein
Substitution_mutations: ( Native_residue: Thr () 141()
Mutant_residue: Asp () 141() ) Heterogen: Monobasi>
>gi|2098315|pdb|1OIB|A Chain A, Phosphate-Binding
Protein Mutant T141d >gi|2098316|pdb|1OIB|B Chain B,
Phosphate-Binding Protein Mutant T141d
Length = 321
Score = 183 bits (460), Expect = 3e-45
Identities = 111/320 (34%), Positives = 168/320 (51%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ + +
Sbjct: 7 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASDAPLSD---E 62
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 63 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 120
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+D SG + +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 121 PGLKLPSQNIAVVRRADGSGDSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 175
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E ++A + NL L + GK V P EE A
Sbjct: 176 GNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 231
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 232 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQGTEVLKFFDWAYKT 283
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 284 GAKQAN-DLDYASLPDSVVE 302
>pdb|1QUL| Phosphate-Binding Protein Mutant With Asp 137 Replaced By Thr
Complex With Chlorine And Phosphate
Length = 321
Score = 183 bits (459), Expect = 4e-45
Identities = 111/320 (34%), Positives = 168/320 (51%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ + +
Sbjct: 7 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASDAPLSD---E 62
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 63 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 120
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+ SGT+ +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 121 PGLKLPSQNIAVVRRATGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 175
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E ++A + NL L + GK V P EE A
Sbjct: 176 GNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 231
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 232 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQGTEVLKFFDWAYKT 283
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 284 GAKQAN-DLDYASLPDSVVE 302
>pdb|1QUI| Phosphate-Binding Protein Mutant With Asp 137 Replaced By Gly
Complex With Bromine And Phosphate >gi|1633134|pdb|1QUJ|
Phosphate-Binding Protein Mutant With Asp 137 Replaced
By Gly Complex With Chlorine And Phosphate
Length = 321
Score = 183 bits (459), Expect = 4e-45
Identities = 111/320 (34%), Positives = 168/320 (51%), Gaps = 26/320 (8%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ + +
Sbjct: 7 AGATFPAPVYAKWADTYQKETGN-KVNYQGIGSSGGVKQIIANTVDFGASDAPLSD---E 62
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ N+P + EL L + L I+LG+I+ WDD AI +LN
Sbjct: 63 KLAQEG--LFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLN 120
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAK 231
P KLP + I V+ R+ SGT+ +FT+YL+ +++EW VG G V WP+ G+G K
Sbjct: 121 PGLKLPSQNIAVVRRAGGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPI-----GLGGK 175
Query: 232 GNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFI 291
GN G+ A +++ +I Y E ++A + NL L + GK V P EE A
Sbjct: 176 GNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGA---- 231
Query: 292 PDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTE 351
D ++ + +D L + GE+++PI + T IL+ +++ PE+ + F W
Sbjct: 232 -DWSKTFAQD---LTNQKGEDAWPITSTTFILIHKDQ----KKPEQGTEVLKFFDWAYKT 283
Query: 352 GQKPENLAPGYVGLPKEVAE 371
G K N Y LP V E
Sbjct: 284 GAKQAN-DLDYASLPDSVVE 302
>pir||G82595 ABC transporter phosphate binding protein XF2141 [imported] -
Xylella fastidiosa (strain 9a5c)
>gi|9107274|gb|AAF84940.1|AE004028_8 (AE004028) ABC
transporter phosphate binding protein [Xylella
fastidiosa]
Length = 364
Score = 182 bits (456), Expect = 8e-45
Identities = 121/333 (36%), Positives = 173/333 (51%), Gaps = 28/333 (8%)
Query: 43 AKNPEIIVIRTSGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGR 102
A+ + + I +GA+F I +W Y + KI Y+ GSG G D G
Sbjct: 45 AEQTKSVKISGAGASFIYPLISQWSADYNAATGN-KINYQSIGSGGGIAQIKAATIDFGS 103
Query: 103 TDPPVKESTWKKFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIE 160
+D P+ S + Q G QFP +G VV V N+ + +L+L+ +LA IFLG+I
Sbjct: 104 SDKPLDSS---ELTQAGLG--QFPSAIGGVVPVVNLDNIEPGKLRLTGPLLADIFLGKIS 158
Query: 161 YWDDPAIKRLNPNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWP 220
W+D AI NP LP KI ++HRSD SGTT F+ YLS +S EW +KVG G V WP
Sbjct: 159 KWNDAAIISANPGLHLPDTKINIVHRSDGSGTTFNFSNYLSKVSAEWKQKVGEGTSVQWP 218
Query: 221 VDKVGRGIGAKGNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETI 280
G+G KGN GV + ++Q K SI Y EL++A++ + AL+N AG++V+P+ E+
Sbjct: 219 -----GGVGGKGNEGVASYVQQIKGSIGYVELAYALQNKMSYTALQNAAGQWVQPSAESF 273
Query: 281 KAAVANVKAFIPDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKA-- 338
AA +N K+ + +A GE ++PI A IL+ + T + A
Sbjct: 274 AAAASNA------DWSNAKDFNLVITNATGEAAWPITATNFILM------RKQTKDAAQR 321
Query: 339 KAIKDFLRWILTEGQKPENLAPGYVGLPKEVAE 371
KA DF +W GQK N YV LP + +
Sbjct: 322 KATLDFFKWSFENGQKQAN-ELHYVPLPPNLVK 353
>pir||I64120 phosphate-binding protein, phosphate-repressible - Haemophilus
influenzae (strain Rd KW20) >gi|1574216|gb|AAC23028.1|
(U32818) phosphate ABC transporter, periplasmic-binding
protein (pstS) [Haemophilus influenzae Rd]
Length = 258
Score = 174 bits (437), Expect = 1e-42
Identities = 106/254 (41%), Positives = 148/254 (57%), Gaps = 24/254 (9%)
Query: 121 QPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPH 178
Q +QFP ++G +V V N+PE+ +LKLS ++LA+IFLG+I+ W+DP + LNP LP+
Sbjct: 10 QLVQFPAVIGGIVPVVNLPEIKPGKLKLSGKLLAEIFLGKIKKWNDPDLVALNPTLPLPN 69
Query: 179 KKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVA 238
K IIVIHRSD SGTT FT YLS IS +W +VG GK V W G G KGN GV +
Sbjct: 70 KNIIVIHRSDGSGTTFGFTNYLSKISNDWKNQVGEGKSVKWLT-----GQGGKGNEGVAS 124
Query: 239 ALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDPTEGY 298
++Q KYSI Y E ++A + L +L+N+AG+FV+P+ E+ AA ++ K +
Sbjct: 125 YVRQMKYSIGYVEYAYAKQNQLAWISLQNQAGQFVQPSNESFMAAASHAK---------W 175
Query: 299 KEDLKQ---LLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKP 355
E L + GE S+PI A + IL+ NK + PE K + F W + GQ
Sbjct: 176 HEKAGMGVILTNETGEKSWPITAASFILL--NKYSDN--PETTKNVLAFFDWAFSRGQDA 231
Query: 356 ENLAPGYVGLPKEV 369
YV +P +V
Sbjct: 232 AT-ELDYVPIPADV 244
>emb|CAB41939.1| (AJ132617) Putative PstS protein [Burkholderia sp.]
Length = 338
Score = 162 bits (405), Expect = 8e-39
Identities = 111/323 (34%), Positives = 161/323 (49%), Gaps = 33/323 (10%)
Query: 51 IRTSGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKES 110
I +G+TF +W Y+K + VK+ Y+G GS G + + D +D P+ +
Sbjct: 25 ITGAGSTFAAPVYAQWAHAYRKASG-VKVNYQGIGSSAGLKQIIAKTVDFAASDTPLTAN 83
Query: 111 TWKKFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIK 168
K LQFP I G VV V NVP + E+ LS +VL I+LG+I W+DP I
Sbjct: 84 ALSKA-----GLLQFPTIAGGVVPVVNVPGIQPGEMVLSGQVLGDIYLGKITKWNDPEII 138
Query: 169 RLNP----NEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKV 224
+LNP +LP I V+ R+D SGT+A++T YLS + +W + VG G V WP
Sbjct: 139 KLNPALHTRARLPDLTIAVVRRADGSGTSALWTHYLSQTNAQWKKTVGEGATVSWPA--- 195
Query: 225 GRGIGAKGNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAV 284
GIG KGN GV A + + K +I Y E S+A + + L N G V PN T KAA+
Sbjct: 196 --GIGGKGNDGVAAFVGRLKGAIGYIEWSYAKQNKISYVRLRNPEGAIVAPNVHTFKAAL 253
Query: 285 ANVKAFIPDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDF 344
+ + + + + P +N++PIV T L+ + + A+A+K F
Sbjct: 254 ERI---------DWTKSSEPMAPIPRQNAWPIVGLTFALL---HATQPVSQNAAQALK-F 300
Query: 345 LRWILTEG-QKPENLAPGYVGLP 366
W G Q +L GY+ LP
Sbjct: 301 FDWSFRNGAQLASDL--GYIMLP 321
>pir||S74876 phosphate-binding periplasmic protein pstS-2 - Synechocystis sp.
(strain PCC 6803) >gi|1652919|dbj|BAA17837.1| (D90909)
periplasmic phosphate binding protein [Synechocystis
sp.]
Length = 333
Score = 153 bits (382), Expect = 4e-36
Identities = 117/337 (34%), Positives = 170/337 (49%), Gaps = 46/337 (13%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GA+FP Q++ +Y+K + + Y GSG G F+ D G TD V S +
Sbjct: 34 AGASFPAPLYQRYFAEYKKATGNT-VNYNSVGSGAGIRQFIGETVDFGATDA-VPTSAQR 91
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPN 173
+ ++ G + P GAV VVYN+P +++LSR L KIF GE++ W+ +
Sbjct: 92 QQMKRG--VVMVPTAGGAVAVVYNLPG-KKVQLSRIALGKIFTGELKTWNQV-------D 141
Query: 174 EKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGN 233
KLP+ I V+ R+D SGTT IFT++LS IS + K+G + +W + G K N
Sbjct: 142 SKLPNTPIRVVVRADGSGTTDIFTSHLSAISSTFRSKIGASQDPNWGFQVLK---GPK-N 197
Query: 234 PGVVAALKQTKYSIAYTELSFAIEE---NLKVAALENKAGKFVKPNEETIKAAV------ 284
GV A +KQT +I Y + +FA NL+ A ++NKAG++V+P+ A+
Sbjct: 198 DGVAATVKQTPGAIGYVQDTFARRNEGPNLQTAKVQNKAGQYVEPSLAEANKAISGAKFD 257
Query: 285 ANVKAFIPDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDF 344
AN A I DP G YPIV T +LV+QN + AK +K
Sbjct: 258 ANFVAEISDPASG----------------YPIVGLTWLLVYQNNKNANV----AKDVKAL 297
Query: 345 LRWILTEGQKPENLAPGYVGLPKEVAEIGLKAVEMIE 381
+RWILT GQ N Y +P VA+ + AV I+
Sbjct: 298 VRWILTTGQN-HNTQLEYTKIPPAVAQRVIAAVNKIK 333
>sp|P15712|PAB_MYCTU PROTEIN ANTIGEN B PRECURSOR (PAB) >gi|98820|pir||A42930 protein
antigen b - Mycobacterium tuberculosis
>gi|2127485|pir||JC5103 phosphate specific transporter S
- Mycobacterium tuberculosis >gi|7428814|pir||F70584
phosphate specific transporter S precursor -
Mycobacterium tuberculosis (strain H37RV)
>gi|149988|gb|AAA25374.1| (M30046) protein antigen b
(gtg start codon) [Mycobacterium tuberculosis]
>gi|2078049|emb|CAB08484.1| (Z95209) phoS1
[Mycobacterium tuberculosis]
Length = 374
Score = 151 bits (378), Expect = 1e-35
Identities = 111/362 (30%), Positives = 171/362 (46%), Gaps = 42/362 (11%)
Query: 11 LLMGLVGVGLLIS--GCIGEETPTHQPKVNS------RSKAKNPEIIVIRTSGATFPQYQ 62
LL L LL++ GC G + P+ P+ + + A +P + S +P +
Sbjct: 8 LLAVLTAAPLLLAAAGC-GSKPPSGSPETGAGAGTVATTPASSPVTLAETGSTLLYPLFN 66
Query: 63 IQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGDQP 122
+ W + + P+V I +G GSG G G +IG +D +L GD
Sbjct: 67 L--WGPAFHERYPNVTITAQGTGSGAGIAQAAAGTVNIGASDA---------YLSEGDMA 115
Query: 123 -----LQFPEIVGAVVVVYNVPEVNE-LKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKL 176
+ + A V YN+P V+E LKL+ +VLA ++ G I+ WDDP I LNP L
Sbjct: 116 AHKGLMNIALAISAQQVNYNLPGVSEHLKLNGKVLAAMYQGTIKTWDDPQIAALNPGVNL 175
Query: 177 PHKKIIVIHRSDASGTTAIFTTYLSLISKE-WAEKVGVGKVVDWPVDKVGRGIGAKGNPG 235
P ++ +HRSD SG T +FT YLS E W + G G VD+P V +G GN G
Sbjct: 176 PGTAVVPLHRSDGSGDTFLFTQYLSKQDPEGWGKSPGFGTTVDFPA--VPGALGENGNGG 233
Query: 236 VVAALKQTKYSIAYTELSF---AIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIP 292
+V +T +AY +SF A + L A L N +G F+ P+ ++I+AA A + P
Sbjct: 234 MVTGCAETPGCVAYIGISFLDQASQRGLGEAQLGNSSGNFLLPDAQSIQAAAAGFASKTP 293
Query: 293 DPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEG 352
++D P + YPI+ + + +V + A+ ++ FL W +T+G
Sbjct: 294 ------ANQAISMIDGPAPDGYPIINYEYAIV----NNRQKDAATAQTLQAFLHWAITDG 343
Query: 353 QK 354
K
Sbjct: 344 NK 345
>emb|CAA64783.1| (X95538) PstS subunit of ABC transporter [Mycobacterium
intracellulare]
Length = 374
Score = 142 bits (354), Expect = 7e-33
Identities = 112/378 (29%), Positives = 171/378 (44%), Gaps = 38/378 (10%)
Query: 12 LMGLVGVG---LLISGCIGEET---PTHQPKVNSRSKAKNPEIIVIRTSGATFPQYQIQK 65
L+GLV + L I+ C T P+ P S + A I + +G+T +
Sbjct: 8 LLGLVAIAPPVLGIAACGAHATRGAPSTGPAAGSVATAPATSTIELSETGSTLLYPLFSE 67
Query: 66 WIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGDQP--- 122
W Y+ +P+V + +G GSG G G IG +D +L GD
Sbjct: 68 WGAAYRAKHPNVTVTTQGSGSGAGISQAAAGAVVIGASDA---------YLSAGDMAAHK 118
Query: 123 --LQFPEIVGAVVVVYNVPEVNE-LKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHK 179
+ + A V YN+P V++ LKL+ +VLA ++ G I+ W+DP I +NP +LP
Sbjct: 119 GLMNIALAISAQQVNYNLPGVHQHLKLNGKVLAGMYRGTIKTWNDPQIAGINPGLQLPAT 178
Query: 180 KIIVIHRSDASGTTAIFTTYLSLISKE-WAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVA 238
++ +HRSD SG+T FT YLS + W G G V +P V +G G+ +V
Sbjct: 179 PVVPLHRSDGSGSTFQFTQYLSKQDPDGWGRSPGFGTTVSFPA--VPEALGENGDGAMVT 236
Query: 239 ALKQTKYSIAYT---ELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDPT 295
+AYT L A + L A L N +GK++ PN ++I+AA A + P
Sbjct: 237 GCAANPGCVAYTSIGSLDEADQHGLGEAKLGNASGKYLLPNAKSIQAAAAGFASKTP--- 293
Query: 296 EGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKP 355
L++ P + YPIV + + +V A+ ++ FL W +TEG P
Sbjct: 294 ---ANQAISLINGPATDGYPIVNYEYAIV----NADQRDDATAQTLQAFLHWAITEGNGP 346
Query: 356 ENL-APGYVGLPKEVAEI 372
L + LP EV +
Sbjct: 347 SFLDKVHFQPLPAEVVTL 364
>gb|AAF74819.1|AF137360_1 (AF137360) periplasmic phosphate permease [Mycobacterium avium]
Length = 369
Score = 140 bits (349), Expect = 3e-32
Identities = 112/351 (31%), Positives = 160/351 (44%), Gaps = 18/351 (5%)
Query: 20 LLISGCIGEETPTHQPKV-NSRSKAKNPEIIVIRTSGATFPQYQIQKWIEKYQKINPHVK 78
L++SGC + +S SK ++ SG+T + +++ +++ P
Sbjct: 18 LVLSGCGSDNNGAGAGAAGSSSSKVSCGGKKALKASGSTAQANAMTRFVNAFEQACPGQT 77
Query: 79 IEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGDQPL-QFPEIVGAVVVVYN 137
+ Y GSG G F TD G +D P+ S + Q P P + G + + YN
Sbjct: 78 LNYTANGSGAGISEFNGKQTDFGGSDSPLAPSEYAAAQQRCGSPAWNLPVVFGPIAITYN 137
Query: 138 VPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHKKIIVIHRSDASGTTAIFT 197
V +N L L AKIF G I W+DP I+ LNP LP + I V+ R+D SGTT F
Sbjct: 138 VAGLNSLNLDGATAAKIFNGAITTWNDPGIQALNPGVALPAEPIHVVFRNDESGTTDNFQ 197
Query: 198 TYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVAALKQTKYSIAYTELSFAIE 257
YL + + A G GK K G G GAKGN G AA+K T+ SI Y E SFA
Sbjct: 198 KYLD-AAADGAWGKGAGKTF-----KGGVGEGAKGNDGTSAAIKATEGSITYNEWSFAQA 251
Query: 258 ENLKVAALENKAG-KFVKPNEETIKAAVANVKAFIPDPTEGYKEDLKQLLDAPGENSYPI 316
+ L +A + AG V + +++ +A K I D SYPI
Sbjct: 252 QKLNMAKIITSAGPDAVAISADSVGKTIAGAK--ISGQGNDLVLDTLSFYKPTQAGSYPI 309
Query: 317 VAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENLAP-GYVGLP 366
V T+ +V K+ P+ A+K FL+ + GQ LA GY+ +P
Sbjct: 310 VLATYEIVC----SKYPDPQVGTAVKAFLQSTVGAGQ--NGLADNGYIPIP 354
>emb|CAB88475.1| (AL353816) phosphate-binding protein precursor [Streptomyces
coelicolor A3(2)]
Length = 370
Score = 139 bits (348), Expect = 4e-32
Identities = 118/377 (31%), Positives = 174/377 (45%), Gaps = 31/377 (8%)
Query: 5 MRWMALLLMGLVGVGLLISGCIGEETPTHQPKVNSRSKAKNPEIIV------IRTSGATF 58
M AL L L G L G + +S S A N I ++ SG++
Sbjct: 1 MNRRALALGALAVSGALALTACGSDDTGGNSGSDSSSAAANSNIKCDDAKGQLQASGSSA 60
Query: 59 PQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKES---TWKKF 115
+ I W+++Y V+I Y GSG G AF +G T +D +K KK
Sbjct: 61 QKNAIDAWVKQYVAACNGVQINYNPTGSGAGITAFTQGQTAFAGSDSALKPDEIEASKKV 120
Query: 116 LQTGDQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEK 175
+ G Q + P + G + V +NV V+ L L +AKIF +I W+D AIK+LNP+ K
Sbjct: 121 CKDG-QAIDLPMVGGPIAVGFNVTGVDSLVLDAPTMAKIFDSKITNWNDEAIKKLNPDAK 179
Query: 176 LPHKKIIVIHRSDASGTTAIFTTYLSLIS-KEWAEKVGVGKVVDWPVDKVGRGIGAKGNP 234
LP KI HRSD SGTT FT YL + +W + G W + G A+G+
Sbjct: 180 LPDLKIQAFHRSDESGTTDNFTKYLKAAAPDDWKYEGG----KSW---EAKGGQSAQGSS 232
Query: 235 GVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDP 294
G+ +KQT +I+Y ELS+A ++ +K ++ A + VK E AA+ K
Sbjct: 233 GLAGQVKQTPGAISYFELSYA-KDGIKTVDVKTAAAEPVKATVENATAAIGAAKVV---- 287
Query: 295 TEGYKEDLKQLLD--APGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEG 352
G +DL LD +YP+V T+ + +KG K + A K FL ++ +E
Sbjct: 288 --GTGKDLALELDYTPDAAGAYPLVLVTYEIAC-DKGNK---ADTLPATKSFLNYMASED 341
Query: 353 QKPENLAPGYVGLPKEV 369
+ GY +P E+
Sbjct: 342 GQGLLADAGYAPMPTEI 358
>gb|AAA63076.1| (U15184) phosphate-repressible, periplasmic binding protein
homologue [Mycobacterium leprae]
Length = 328
Score = 138 bits (343), Expect = 1e-31
Identities = 110/325 (33%), Positives = 154/325 (46%), Gaps = 29/325 (8%)
Query: 51 IRTSGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKES 110
++ SG+T + +++ +++ P + Y GSG G F TD G +D P+
Sbjct: 9 LKASGSTAQANAMTRFVNAFERSCPGQTLNYTANGSGAGVSEFNGNQTDFGGSDSPLSRK 68
Query: 111 TWKKFLQT-GDQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKR 169
+ Q G Q P + G + + YNV ++ L L AKIF G I W+DPAI+
Sbjct: 69 EYAAAEQRCGSQAWNLPVVFGPIAITYNVNGLSSLNLDGPTTAKIFNGSIASWNDPAIQA 128
Query: 170 LNPNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISK-EWAEKVGVGKVVDWPVDKVGRGI 228
LN LP + I V+ R+D SGTT F YL + S EW + G+GK K G G
Sbjct: 129 LNTGVALPAEPIHVVFRNDESGTTDNFQRYLDVASNGEWGK--GIGKTF-----KGGVGE 181
Query: 229 GAKGNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVK 288
GAKGN G AA+K T+ SI Y E SFA L A + A + E I +V +V
Sbjct: 182 GAKGNDGTSAAVKSTEGSITYNEWSFASARKLNTAKIATSA------DPEPIAISVDSVG 235
Query: 289 AFIPDPT-EGYKEDLKQLLDA-----PGE-NSYPIVAFTHILVWQNKGGKHYTPEKAKAI 341
I T G DL +LD P + SYPIV T+ +V K+ + +A+
Sbjct: 236 KTISGATIIGEGNDL--VLDTVSFYKPAQPGSYPIVLATYEIVC----SKYPDAQVGRAV 289
Query: 342 KDFLRWILTEGQKPENLAPGYVGLP 366
K FL+ + GQ GYV +P
Sbjct: 290 KAFLQSTIGGGQNGLG-DNGYVPIP 313
>pir||S54862 PstS-3 protein precursor - Mycobacterium tuberculosis
>gi|840827|emb|CAA88138.1| (Z48057) Ag88 [Mycobacterium
tuberculosis]
Length = 369
Score = 133 bits (332), Expect = 3e-30
Identities = 113/367 (30%), Positives = 168/367 (44%), Gaps = 31/367 (8%)
Query: 13 MGLVGVG-LLISGCIGEETPTHQPKVNSRSKAKNP--EIIVIRTSGATFPQYQIQKWIEK 69
+G++ G L++S C ++ T ++ AK ++ SG+T + +++
Sbjct: 10 VGVLAAGALVLSACGNDDNVTGGGATTGQASAKVDCGGKKTLKASGSTAQANAMTRFVNV 69
Query: 70 YQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGDQPLQFPEIV 129
+++ P + Y GSG G F TD G +D P+ + + G P +
Sbjct: 70 FEQACPGQTLNYTANGSGAGISEFNGNQTDFGGSDVPLSKDEPSGAARCGSPAWNLPVVF 129
Query: 130 GAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHKKIIVIHRSDA 189
G + V YN+ V+ L L LAKIF G I W++PAI+ LN + LP ++I V+ RSD
Sbjct: 130 GPIAVTYNLNSVSSLNLDGPTLAKIFNGSITQWNNPAIQALNRDFTLPGERIHVVFRSDE 189
Query: 190 SGTTAIFTTYLSLISK-EWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVAALKQTKYSIA 248
SGTT F YL S W + G GK + G G GA+GN G AA K T SI
Sbjct: 190 SGTTDNFQRYLQAASNGAWGK--GAGKSF-----QGGVGEGARGNDGTSAAAKNTPGSIT 242
Query: 249 YTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDPT-EGYKEDLKQLLD 307
Y E SFA +L +A + AG + + + +V I T G DL D
Sbjct: 243 YNEWSFAQAHDLTMANIVTSAG------GDPVAITIDSVGQTIAGATISGVGNDLVLDTD 296
Query: 308 A------PGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENLAPG 361
+ PG SYPIV T+ +V K+ + A+K FL+ + GQ G
Sbjct: 297 SFYRPKRPG--SYPIVLATYEIVC----SKYPDSQVGTAVKAFLQSTIGAGQSGLG-DNG 349
Query: 362 YVGLPKE 368
Y+ +P E
Sbjct: 350 YIPIPDE 356
>gb|AAA62993.1| (U15182) match to phosphate-repressible, periplasmic
phosphate-binding prot [Mycobacterium leprae]
Length = 429
Score = 132 bits (330), Expect = 5e-30
Identities = 111/383 (28%), Positives = 176/383 (44%), Gaps = 23/383 (6%)
Query: 6 RWMALLLMGLVGVGLLISGCIGEETPTHQPKVNSRSKAKNPEII----VIRTSGATFPQY 61
R A++L V G+L S C + H + K+ + + G+T Q
Sbjct: 63 RAFAMVLTTAVTAGVL-SACGSDVNRGHTATTSISGKSADTAACGGKRTMTAEGSTAQQN 121
Query: 62 QIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQT--G 119
I + + + K+ P + Y GSG G+E F+ G D +D P+ + G
Sbjct: 122 AIALFNQIWGKLCPDKTVSYNPTGSGAGREQFIAGRIDFAGSDSPLLADQLGPAAKRCKG 181
Query: 120 DQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHK 179
+ P + G V +VYN+P V L ++ +VLAKIF G I W+D + LNP LP+
Sbjct: 182 NPAWDLPLVFGPVALVYNLPGVQMLTVNSDVLAKIFSGVITTWNDGILAALNPGVVLPNL 241
Query: 180 KIIVIHRSDASGTTAIFTTYLSL-ISKEWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVA 238
+I+ I+R+DASGTT YL+ + WA+ VG + G G G + + GV+
Sbjct: 242 RIMPIYRADASGTTDNLQKYLTTDAPQSWAKGVGA-------EFQGGVGEGVQKSAGVIQ 294
Query: 239 ALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDPTEGY 298
A++ T + Y E SFA L A ++ +G V ++T + + N F+ +
Sbjct: 295 AVQTTPGAFGYVEKSFADRAGLPFAQIDTGSGG-VPLTDDTARNGI-NAANFVSVGND-L 351
Query: 299 KEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENL 358
DL + N+YP+V T+ +V K Y P+ AIK FL +GQ
Sbjct: 352 VLDLNAMYSTEQSNAYPLVLATYEIVC----SKGYDPDTFAAIKSFLTVAANDGQ-AGLA 406
Query: 359 APGYVGLPKEVAEIGLKAVEMIE 381
A GYV LP + + + A+ ++
Sbjct: 407 AVGYVPLPDTIKKRLVSAINAMQ 429
>emb|CAA64784.1| (X95538) PstS subunit of ABC transporter [Mycobacterium
intracellulare]
Length = 374
Score = 132 bits (328), Expect = 8e-30
Identities = 109/384 (28%), Positives = 171/384 (44%), Gaps = 39/384 (10%)
Query: 5 MRWMALLLMGLVGVGLLISGCIGEETPTHQPKVN-----SRSKAKNPEIIVIRTSGATFP 59
+R LL M + + G + T P + + A +P + + +G+T
Sbjct: 3 VRMSRLLAMAATAPLVFAAAACGSNSQTGAPSQGGGGPVATTPASSP--VTLSETGSTLL 60
Query: 60 QYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTG 119
W Y +V I +G GSG G G IG +D +L G
Sbjct: 61 YPLFNLWGPAYHDKYSNVTITTQGTGSGTGISQAAAGAVAIGASDA---------YLSEG 111
Query: 120 DQP-----LQFPEIVGAVVVVYNVPEVNE-LKLSREVLAKIFLGEIEYWDDPAIKRLNPN 173
D + + A V YN+P V E +KL+ +VLA ++ G ++ W+DP I LNP
Sbjct: 112 DMAAHKGLMNIALAISAQQVNYNLPGVTEHIKLNGKVLAGMYNGSVKTWNDPQIAGLNPG 171
Query: 174 EKLPHKKIIVIHRSDASGTTAIFTTYLSLISKE-WAEKVGVGKVVDWPVDKVGRGIGAKG 232
LP ++ +HRSD SG T +FT YLS + W + G G V +P V +G G
Sbjct: 172 VNLPATPVVPLHRSDGSGDTFLFTQYLSKQDPDGWGKSPGFGTTVAFPT--VPGALGENG 229
Query: 233 NPGVVAALKQTKYSIAYTELSF---AIEENLKVAALENKAGKFVKPNEETIKAAVANVKA 289
N G+V T +AY +SF A + L A L N + K++ P+ ++I+AA A +
Sbjct: 230 NGGMVTGCADTPGCVAYIGISFLDQAQGKGLGEAQLANASDKYLLPDAKSIQAAAAGFAS 289
Query: 290 FIPDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWIL 349
P L++ P + YPIV + + +V N G K A+ ++ FL W +
Sbjct: 290 KTP------ANQAISLINGPAPDGYPIVNYEYAIV--NSGQK--DAATAQTLQAFLHWAI 339
Query: 350 TEGQKPENL-APGYVGLPKEVAEI 372
++G L + LP +VA++
Sbjct: 340 SDGNNSSFLDKVHFQPLPADVAKL 363
>gb|AAC45381.1| (U75514) PstS [Prochlorococcus marinus]
Length = 331
Score = 132 bits (328), Expect = 8e-30
Identities = 101/374 (27%), Positives = 172/374 (45%), Gaps = 54/374 (14%)
Query: 13 MGLVGVGLLISGCIGEETPTHQPKVNSRSKAKNPEIIVIRTSGATFPQYQIQKWIEKYQK 72
M + L+ + + T P + +K + + +GA+FP +W +
Sbjct: 4 MNIAKKALVFTSAVAIAAGTSVPGTSVSAKTR------LSGAGASFPAKIYTRWFKDLAS 57
Query: 73 INPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWKKFLQTGDQPLQFPEIVGAV 132
+ ++ Y+ GSG G++AF+ + G +D P+K+ K + +Q P + G +
Sbjct: 58 -SGGPRVNYQAVGSGSGRKAFIDQTVNFGASDDPMKD---KDIAKVTRGLVQIPMVGGTI 113
Query: 133 VVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHKKIIVIHRSDASGT 192
YN +LKL++E ++ +G ++ W + K K+ HRSD SGT
Sbjct: 114 AFGYNYD--CDLKLTQEQAVRVAMGMVKNWKELGCK---------SGKLTWAHRSDGSGT 162
Query: 193 TAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVAALKQTKYSIAYTEL 252
T FT + S W +G GK V WP G+GAKGN GV ++ T +I Y
Sbjct: 163 TKAFTNSMEAFSPTWT--LGTGKSVKWPA-----GVGAKGNSGVAGVIQNTPGAIGYVNQ 215
Query: 253 SFAIEENLKVAALENKAGKFVKPNEETIKAAV------ANVKAFIPDPTEGYKEDLKQLL 306
S+ I+ N+K AAL+N +G+F+KP+ E A+ N+ P+PT
Sbjct: 216 SY-IKGNVKAAALQNLSGEFLKPSVEAGAKALNGITLDENLAGKNPNPT----------- 263
Query: 307 DAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENLAPGYVGLP 366
+ +YPI + T IL ++ G++ KAIK +L++ + + + G+V L
Sbjct: 264 ---AKGAYPIASLTWILAYEEGNGRN-----TKAIKQAFNTLLSDEYQDKAPSLGFVPLK 315
Query: 367 KEVAEIGLKAVEMI 380
++ E AV+ I
Sbjct: 316 GDILEKSRAAVKKI 329
>pir||H70583 phosphate-binding protein S-3 precursor, periplasmic -
Mycobacterium tuberculosis >gi|3261751|emb|CAB08488.1|
(Z95209) phoS2 [Mycobacterium tuberculosis]
Length = 370
Score = 132 bits (328), Expect = 8e-30
Identities = 114/368 (30%), Positives = 170/368 (45%), Gaps = 32/368 (8%)
Query: 13 MGLVGVG-LLISGCIGEETPTHQPKVNSRSKAKNP--EIIVIRTSGATFPQYQIQKWIEK 69
+G++ G L++S C ++ T ++ AK ++ SG+T + +++
Sbjct: 10 VGVLAAGALVLSACGNDDNVTGGGATTGQASAKVDCGGKKTLKASGSTAQANAMTRFVNV 69
Query: 70 YQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPV-KESTWKKFLQTGDQPLQFPEI 128
+++ P + Y GSG G F TD G +D P+ K+ + G P +
Sbjct: 70 FEQACPGQTLNYTANGSGAGISEFNGNQTDFGGSDVPLSKDEAAAAQRRCGSPAWNLPVV 129
Query: 129 VGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPNEKLPHKKIIVIHRSD 188
G + V YN+ V+ L L LAKIF G I W++PAI+ LN + LP ++I V+ RSD
Sbjct: 130 FGPIAVTYNLNSVSSLNLDGPTLAKIFNGSITQWNNPAIQALNRDFTLPGERIHVVFRSD 189
Query: 189 ASGTTAIFTTYLSLISK-EWAEKVGVGKVVDWPVDKVGRGIGAKGNPGVVAALKQTKYSI 247
SGTT F YL S W + G GK + G G GA+GN G AA K T SI
Sbjct: 190 ESGTTDNFQRYLQAASNGAWGK--GAGKSF-----QGGVGEGARGNDGTSAAAKNTPGSI 242
Query: 248 AYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPDPT-EGYKEDLKQLL 306
Y E SFA ++L +A + AG + + + +V I T G DL
Sbjct: 243 TYNEWSFAQAQHLTMANIVTSAG------GDPVAITIDSVGQTIAGATISGVGNDLVLDT 296
Query: 307 DA------PGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQKPENLAP 360
D+ PG SYPIV T+ +V K+ + A+K FL+ + GQ
Sbjct: 297 DSFYRPKRPG--SYPIVLATYEIVC----SKYPDSQVGTAVKAFLQSTIGAGQSGLG-DN 349
Query: 361 GYVGLPKE 368
GY+ +P E
Sbjct: 350 GYIPIPDE 357
>pir||S39852 phosphate-binding protein, periplasmic - Synechococcus sp
>gi|296206|emb|CAA50495.1| (X71359) periplasmic
phosphate binding protein [Synechococcus sp.]
Length = 326
Score = 125 bits (311), Expect = 8e-28
Identities = 94/327 (28%), Positives = 151/327 (45%), Gaps = 33/327 (10%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GA+FP Q+W + K ++ Y+ GSG G++AF+ + G +D P+K
Sbjct: 31 AGASFPAKIYQRWFAELAKAGGP-QVNYQAVGSGSGRKAFIDQTVNFGASDDPMKPKDMA 89
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAIKRLNPN 173
K + +Q P + G + YN P N LKL+++ ++ +G+I W N
Sbjct: 90 KVKRG---VVQIPMVGGTIAFGYNKPGCN-LKLTQKQAVQVAIGKITNWKQLGCAPGN-- 143
Query: 174 EKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGRGIGAKGN 233
I +HRSD SGTT FT + S EW +G GK V WP +GAKGN
Sbjct: 144 -------ITWVHRSDGSGTTKAFTNSMQAFSSEWT--LGTGKSVKWPGK---NAVGAKGN 191
Query: 234 PGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETIKAAVANVKAFIPD 293
GV ++ +I Y S+ I+ + AAL+NK+G+F+KP ++KA + D
Sbjct: 192 SGVAGTIQNKVGAIGYVNQSY-IKGKVVAAALQNKSGEFLKP---SVKAGATALNGIKLD 247
Query: 294 PTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFLRWILTEGQ 353
K + + +YPI T +L ++ GK+ AIK+ ++L+
Sbjct: 248 SNLAGKNP-----NPSAKGAYPIATLTWVLAYKTGNGKN-----TAAIKEAFNFMLSNKA 297
Query: 354 KPENLAPGYVGLPKEVAEIGLKAVEMI 380
+ + + G+V L + AV I
Sbjct: 298 QDQAPSLGFVPLKGGILSKAKAAVNKI 324
>pir||D70584 phosphate uptake system component pstS-2 precursor - Mycobacterium
tuberculosis (strain H37RV) >gi|2078051|emb|CAB08486.1|
(Z95209) pstS [Mycobacterium tuberculosis]
>gi|2208958|emb|CAA88137.1| (Z48056) PstS-2
[Mycobacterium tuberculosis]
Length = 370
Score = 125 bits (310), Expect = 1e-27
Identities = 98/322 (30%), Positives = 145/322 (44%), Gaps = 19/322 (5%)
Query: 51 IRTSGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKES 110
+ +SG+T + +++++ Y + P ++Y GSG G FL TD +D P+ S
Sbjct: 49 LHSSGSTAQENAMEQFVYAYVRSCPGYTLDYNANGSGAGVTQFLNNETDFAGSDVPLNPS 108
Query: 111 TW---KKFLQTGDQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAI 167
T + + G P + G + + YN+ V+ L L AKIF G I W+DP I
Sbjct: 109 TGQPDRSAERCGSPAWDLPTVFGPIAITYNIKGVSTLNLDGPTTAKIFNGTITVWNDPQI 168
Query: 168 KRLNPNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISK-EWAEKVGVGKVVDWPVDKVGR 226
+ LN LP I VI RSD SGT+ F YL S W + G + + G
Sbjct: 169 QALNSGTDLPPTPISVIFRSDKSGTSDNFQKYLDGASNGAWGK--GASETFNG-----GV 221
Query: 227 GIGAKGNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKF-VKPNEETIKAAVA 285
G+GA GN G A L+ T SI Y E SFA+ + L +A + AG V E++ +A
Sbjct: 222 GVGASGNNGTSALLQTTDGSITYNEWSFAVGKQLNMAQIITSAGPDPVAITTESVGKTIA 281
Query: 286 NVKAFIPDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFL 345
K I D SYPIV T+ +V K+ A++ F+
Sbjct: 282 GAK--IMGQGNDLVLDTSSFYRPTQPGSYPIVLATYEIVC----SKYPDATTGTAVRAFM 335
Query: 346 RWILTEGQKPENLAPGYVGLPK 367
+ + GQ+ + G + LPK
Sbjct: 336 QAAIGPGQEGLD-QYGSIPLPK 356
>pir||S54861 PstS-2 protein precursor - Mycobacterium tuberculosis
Length = 370
Score = 124 bits (308), Expect = 2e-27
Identities = 96/316 (30%), Positives = 142/316 (44%), Gaps = 19/316 (6%)
Query: 51 IRTSGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKES 110
+ +SG+T + +++++ Y + P ++Y GSG G FL TD +D P+ S
Sbjct: 49 LHSSGSTAQENAMEQFVYAYVRSCPGYTLDYNANGSGAGVTQFLNNETDFAGSDVPLNPS 108
Query: 111 TW---KKFLQTGDQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPAI 167
T + + G P + G + + YN+ V+ L L AKIF G I W+DP I
Sbjct: 109 TGQPDRSAERCGSPAWDLPTVFGPIAITYNIKGVSTLNLDGPTTAKIFNGTITVWNDPQI 168
Query: 168 KRLNPNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISK-EWAEKVGVGKVVDWPVDKVGR 226
+ LN LP I VI RSD SGT+ F YL S W + G + + G
Sbjct: 169 QALNSGTDLPPTPISVIFRSDKSGTSDNFQKYLDGASNGAWGK--GASETFNG-----GV 221
Query: 227 GIGAKGNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKF-VKPNEETIKAAVA 285
G+GA GN G A L+ T SI Y E SFA+ + L +A + AG V E++ +A
Sbjct: 222 GVGASGNNGTSALLQTTDGSITYNEWSFAVGKQLNMAQIITSAGPDPVAITTESVGKTIA 281
Query: 286 NVKAFIPDPTEGYKEDLKQLLDAPGENSYPIVAFTHILVWQNKGGKHYTPEKAKAIKDFL 345
K I D SYPIV T+ +V K+ A++ F+
Sbjct: 282 GAK--IMGQGNDLVLDTSSFYRPTQPGSYPIVLATYEIVC----SKYPDATTGTAVRAFM 335
Query: 346 RWILTEGQKP-ENLAP 360
+ + + +K N AP
Sbjct: 336 QAAIGQAKKAWTNTAP 351
>gb|AAB70458.1| (U70621) phosphate-binding protein precursor [Pectobacterium
carotovorum subsp. carotovorum]
Length = 179
Score = 116 bits (288), Expect = 4e-25
Identities = 63/155 (40%), Positives = 90/155 (57%), Gaps = 8/155 (5%)
Query: 54 SGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPPVKESTWK 113
+GATFP KW + YQK + K+ Y+G GS G + + D G +D P+ +
Sbjct: 31 AGATFPAPVYAKWADSYQKETGN-KVNYQGIGSSGGVKQIIAKTVDFGASDAPLADD--- 86
Query: 114 KFLQTGDQPLQFPEIVGAVVVVYNVPEVN--ELKLSREVLAKIFLGEIEYWDDPAIKRLN 171
K Q G QFP ++G VV+ NVP + +L L + L I+LG I W DPAI +LN
Sbjct: 87 KLAQDG--LFQFPTVIGGVVLAVNVPGIKSGDLTLDGKTLGDIYLGNINKWSDPAITKLN 144
Query: 172 PNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKE 206
PN KLP + I V+ R+D SGT+ +FT+YL+ ++ E
Sbjct: 145 PNAKLPDQDIAVVRRADGSGTSFVFTSYLAKVNPE 179
>pir||C72276 phosphate ABC transporter, periplasmic phosphate-binding protein -
Thermotoga maritima (strain MSB8)
>gi|4981819|gb|AAD36338.1|AE001781_9 (AE001781)
phosphate ABC transporter, periplasmic phosphate-binding
protein [Thermotoga maritima]
Length = 274
Score = 84.3 bits (205), Expect = 2e-15
Identities = 72/257 (28%), Positives = 120/257 (46%), Gaps = 36/257 (14%)
Query: 47 EIIVIRTSGATFPQYQIQKWIEKYQKINPHVKIEYEGGGSGYGQEAFLKGLTDIGRTDPP 106
E +VI+ S FP Q+ WIE+++K++P +++ EG GS G A G TDI +
Sbjct: 19 ETLVIKGSNTVFPIAQL--WIEEFKKLHPDLQVTLEGAGSSTGIAALFNGTTDIANSSRW 76
Query: 107 VKESTWKKFLQTGDQPLQFPEIVGAVVVVYNVPEVNELKLSREVLAKIFLGEIEYWDDPA 166
+K S ++ + G + F + ++ N ++ +S E L KI+ GEI+YW
Sbjct: 77 LKPSEIERMNKEGKYFIPFLIAFDGIAIIVN-KDLGIDDISIETLKKIYTGEIQYW---- 131
Query: 167 IKRLNPNEKLPHKKIIVIHRSDASGTTAIFTTYLSLISKEWAEKVGVGKVVDWPVDKVGR 226
++NPN LP ++I+V R+ ASGT F + L A V + + V+ V +
Sbjct: 132 -SQVNPN--LPKQRIVVYSRNTASGTYETFENKV-LGGARMAPYVRMVESTQLEVESVSK 187
Query: 227 GIGAKGNPGVVAALKQTKYSIAYTELSFAIEENLKVAALENKAGKFVKPNEETI---KAA 283
NP Y+IAY + + + +++KV + + P +E I K
Sbjct: 188 ------NP----------YAIAYVGVGY-VTDDVKVLKVNG-----IYPTKENILKGKYP 225
Query: 284 VANVKAFIPDPTEGYKE 300
+A D T G+ E
Sbjct: 226 IARPLFMFVDATNGFPE 242
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.315 0.136 0.399
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 154675372
Number of Sequences: 2977
Number of extensions: 6868281
Number of successful extensions: 12127
Number of sequences better than 1.0e-10: 39
Number of HSP's better than 0.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 11957
Number of HSP's gapped (non-prelim): 39
length of query: 383
length of database: 189,106,746
effective HSP length: 56
effective length of query: 327
effective length of database: 155,591,474
effective search space: 50878411998
effective search space used: 50878411998
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 42 (22.0 bits)
S2: 166 (69.1 bits)