BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2366 (phoA) DE:ALKALINE PHOSPHATASE IV PRECURSOR (EC
3.1.3.1) (APASE IV)
(495 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75081 alkaline phosphatase (EC 3.1.3.1) IV PAB2366 precurs... 975 0.0
pir||B72410 alkaline phosphatase - Thermotoga maritima (strain M... 300 2e-80
sp|P19406|PPB4_BACSU ALKALINE PHOSPHATASE IV PRECURSOR (APASE IV... 276 4e-73
pir||A39096 alkaline phosphatase (EC 3.1.3.1) IV precursor - Bac... 276 4e-73
dbj|BAB04593.1| (AP001510) alkaline phosphatase [Bacillus halodu... 265 9e-70
gb|AAG33874.1|AF320303_1 (AF320303) alkaline phosphatase [Lactob... 261 1e-68
pir||C69676 alkaline phosphatase (EC 3.1.3.1) III precursor [val... 255 7e-67
gb|AAD38069.1|AF154110_1 (AF154110) alkaline phosphatase [Entero... 254 2e-66
gb|AAD53269.1|AF167172_1 (AF167172) alkaline phosphatase [Clonin... 254 2e-66
sp|P19405|PPB3_BACSU ALKALINE PHOSPHATASE III PRECURSOR (APASE I... 250 2e-65
gb|AAG20936.1| (AE005160) alkaline phosphatase; Aph [Halobacteri... 217 2e-55
gb|AAG10093.1|U79570_2 (U79570) alkaline phosphatase I [Bacillus... 207 3e-52
gb|AAC31201.1| (AF079878) alkaline phosphatase [Thermus sp. FD3041] 182 8e-45
gb|AAF13361.1|AF168770_1 (AF168770) alkaline phosphatase [Thermu... 178 2e-43
gi|6320689 repressible alkaline phosphatase; Pho8p [Saccharomyce... 175 1e-42
gb|AAA34871.1| (M21134) repressible alkaline phosphatase (EC 3.1... 173 4e-42
emb|CAB82508.1| (Y18016) alkaline phosphatase [Antarctic bacteri... 159 7e-38
gb|AAF48480.1| (AE003499) CG8105 gene product [Drosophila melano... 158 1e-37
pir||H82777 alkaline phosphatase XF0657 [imported] - Xylella fas... 150 4e-35
gb|AAF55830.1| (AE003733) CG10827 gene product [Drosophila melan... 149 8e-35
dbj|BAA92181.1| (AB031539) endoderm-specific alkaline phosphatas... 148 1e-34
sp|P19147|PPB_SERMA ALKALINE PHOSPHATASE PRECURSOR (APASE) >gi|1... 148 1e-34
pir||T39459 repressible alkaline phosphatase precursor - fission... 148 2e-34
emb|CAA67052.1| (X98402) alkaline phosphatase [Drosophila melano... 146 7e-34
sp|Q92058|PPBT_CHICK ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC IS... 144 2e-33
pdb|1ANI|A Chain A, Alkaline Phosphatase (D153h, K328h) >gi|1310... 144 3e-33
sp|P21948|PPB_ESCFE ALKALINE PHOSPHATASE PRECURSOR (APASE) >gi|2... 143 6e-33
gb|AAF57106.1| (AE003776) Aph-4 gene product [Drosophila melanog... 142 7e-33
gb|AAF58927.1| (AE003833) CG1809 gene product [Drosophila melano... 141 1e-32
gb|AAD37431.1|AF149417_1 (AF149417) alkaline phosphatase [Canis ... 141 2e-32
sp|P09487|PPBT_BOVIN ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC IS... 141 2e-32
pdb|1ANJ|A Chain A, Alkaline Phosphatase (K328h) >gi|1310909|pdb... 140 3e-32
pdb|2ANH|A Chain A, Alkaline Phosphatase (D153h) >gi|1310739|pdb... 140 3e-32
gi|7106246 tissue-nonspecific ALP alkaline phosphatase [Rattus n... 140 3e-32
sp|Q29486|PPBT_FELCA ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC IS... 140 3e-32
sp|P08289|PPBT_RAT ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC ISOZ... 140 3e-32
gb|AAF50764.1| (AE003565) CG5150 gene product [Drosophila melano... 140 3e-32
gi|4502063 alkaline phosphatase, liver/bone/kidney; tissue-nonsp... 139 6e-32
emb|CAA07382.1| (AJ006985) StrK [Streptomyces glaucescens] 139 6e-32
gi|11424592 alkaline phosphatase, liver/bone/kidney [Homo sapiens] 139 6e-32
pir||A83235 alkaline phosphatase PA3296 [imported] - Pseudomonas... 139 8e-32
sp|P05186|PPBT_HUMAN ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC IS... 139 8e-32
dbj|BAA32129.1| (AB011406) alkalin phosphatase [Homo sapiens] 139 8e-32
gb|AAF64516.1| (AF250846) tissue non-specific alkaline phosphata... 138 1e-31
gb|AAF51724.1| (AE003594) CG5656 gene product [Drosophila melano... 138 1e-31
gb|AAC33182.1| (U35316) alkaline phosphatase with N-terminal Pel... 138 1e-31
pir||S00289 alkaline phosphatase (EC 3.1.3.1), hepatic precursor... 138 2e-31
pdb|1ALK|A Chain A, Alkaline Phosphatase (E.C.3.1.3.1) >gi|49381... 138 2e-31
pdb|1AJB|A Chain A, Mol_id: 1; Molecule: Alkaline Phosphatase; C... 138 2e-31
gb|AAF53795.1| (AE003662) CG16771 gene product [Drosophila melan... 137 3e-31
dbj|BAA92180.1| (AB031538) endoderm-specific alkaline phosphatas... 137 3e-31
pir||PAECA alkaline phosphatase (EC 3.1.3.1) precursor - Escheri... 137 3e-31
sp|P00634|PPB_ECOLI ALKALINE PHOSPHATASE PRECURSOR (APASE) >gi|1... 137 3e-31
pir||I79530 alkaline phosphatase (EC 3.1.3.1) precursor - Escher... 137 3e-31
gb|AAB60529.1| (U25548) alkaline phosphatase [synthetic construct] 137 3e-31
gb|AAC53686.1| (U50975) phoA gene product [Cloning vector pFW_ph... 137 3e-31
pdb|1ALI|A Chain A, Alkaline Phosphatase Mutant (H412n) >gi|1311... 137 3e-31
pdb|1HQA|A Chain A, Alkaline Phosphatase (H412q) >gi|1421391|pdb... 137 3e-31
gb|AAC73486.1| (AE000145) alkaline phosphatase [Escherichia coli... 137 3e-31
gb|AAF80181.1|AF247169_1 (AF247169) alkaline phosphatase [Expres... 137 3e-31
pdb|1ED8|A Chain A, Structure Of E. Coli Alkaline Phosphatase In... 137 3e-31
pir||I79526 alkaline phosphatase (EC 3.1.3.1) precursor - Escher... 136 4e-31
pir||I79529 alkaline phosphatase (EC 3.1.3.1) precursor - Escher... 136 6e-31
pdb|1ALH|A Chain A, Alkaline Phosphatase (E.C.3.1.3.1) Mutant Wi... 136 6e-31
dbj|BAA13488.1| (D87872) HrES-AP [Halocynthia roretzi] 136 6e-31
gb|AAF50765.1| (AE003565) CG10592 gene product [Drosophila melan... 136 7e-31
pir||I79527 alkaline phosphatase (EC 3.1.3.1) precursor - Escher... 136 7e-31
gi|6671533 alkaline phosphatase 2, liver; Tissue Non-Specific Al... 136 7e-31
gb|AAC33184.1| (U94950) modified E.coli alkaline phosphatase wit... 136 7e-31
pdb|1ELX|A Chain A, E. Coli Alkaline Phosphatase Mutant (S102a) ... 136 7e-31
pir||A40172 alkaline phosphatase (EC 3.1.3.1) precursor - mouse 136 7e-31
gb|AAA24363.1| (M29663) alkaline phosphatase precursor (EC 3.1.3... 135 1e-30
pdb|1ELZ|A Chain A, E. Coli Alkaline Phosphatase Mutant (S102g) ... 135 1e-30
pdb|1B8J|A Chain A, Alkaline Phosphatase Complexed With Vanadate... 135 1e-30
pdb|1URB|A Chain A, Alkaline Phosphatase (N51mg) >gi|1633108|pdb... 135 1e-30
pdb|1ELY|A Chain A, E. Coli Alkaline Phosphatase Mutant (S102c) ... 135 1e-30
gi|6671537 alkaline phosphatase 5 [Mus musculus] >gi|130742|sp|P... 134 2e-30
sp|P09401|STRK_STRGR STREPTOMYCIN-6-PHOSPHATE PHOSPHATASE PRECUR... 134 2e-30
pir||I79528 alkaline phosphatase (EC 3.1.3.1) precursor - Escher... 134 3e-30
gb|AAF36717.1|AF227507_1 (AF227507) intestinal alkaline phosphat... 133 4e-30
emb|CAA37374.1| (X53279) protein precursor (AA -19 to 513) [Homo... 132 8e-30
pdb|1HJK|A Chain A, Alkaline Phosphatase Mutant H331q >gi|262484... 132 8e-30
pir||S12076 alkaline phosphatase (EC 3.1.3.1) precursor, placent... 132 1e-29
gb|AAC32393.1| (AF083070) alkaline phosphatase [Expression vecto... 131 1e-29
gb|AAF64515.1| (AF250845) alkaline phosphatase [Canis familiaris] 131 1e-29
sp|P15693|PPBI_RAT ALKALINE PHOSPHATASE, INTESTINAL 1 PRECURSOR ... 131 1e-29
pir||I37991 alkaline phosphatase - human >gi|296798|emb|CAA39425... 131 1e-29
gb|AAA51704.1| (M21499) intestinal alkaline phosphatase precurso... 131 2e-29
sp|P19111|PPBI_BOVIN ALKALINE PHOSPHATASE, INTESTINAL PRECURSOR ... 131 2e-29
gi|4502065 alkaline phosphatase, placental (Regan isozyme) [Homo... 131 2e-29
sp|P05187|PPB1_HUMAN ALKALINE PHOSPHATASE, PLACENTAL TYPE 1 PREC... 131 2e-29
gb|AAA51700.1| (J04948) alkaline phosphatase precursor (EC 3.1.3... 131 2e-29
gb|AAA51708.1| (M14169) preplacental alkaline phosphatase type 1... 131 2e-29
gb|AAC97139.1| (M13078) alkaline phosphatase precursor [Homo sap... 131 2e-29
gb|AAB64400.1| (U89937) secreted alkaline phosphatase [unidentif... 131 2e-29
sp|P09923|PPBI_HUMAN ALKALINE PHOSPHATASE, INTESTINAL PRECURSOR ... 130 3e-29
gi|4502061 alkaline phosphatase, intestinal [Homo sapiens] >gi|1... 130 3e-29
sp|P10696|PPBN_HUMAN ALKALINE PHOSPHATASE, PLACENTAL-LIKE PRECUR... 130 3e-29
sp|P06861|PPB2_HUMAN ALKALINE PHOSPHATASE, PLACENTAL PRECURSOR (... 129 5e-29
gb|AAA98616.1| (J03252) alkaline phosphatase [Homo sapiens] 129 7e-29
gi|11428784 alkaline phosphatase, intestinal [Homo sapiens] 127 2e-28
gb|AAF46819.1| (AE003456) CG3292 gene product [Drosophila melano... 127 3e-28
sp|P29523|PPB_BOMMO MEMBRANE-BOUND ALKALINE PHOSPHATASE PRECURSO... 126 5e-28
gb|AAC33854.1| (AF052227) intestinal alkaline phosphatase IV; IA... 125 1e-27
gi|6671535 alkaline phosphatase 3, intestine, not Mn requiring [... 121 2e-26
gb|AAF36718.1|AF227508_1 (AF227508) intestinal alkaline phosphat... 120 5e-26
gb|AAF46820.1| (AE003456) CG3290 gene product [Drosophila melano... 118 1e-25
sp|P51740|PPBJ_RAT ALKALINE PHOSPHATASE, INTESTINAL 2 PRECURSOR ... 115 9e-25
dbj|BAA34926.1| (AB013386) soluble alkaline phosphatase [Bombyx ... 114 2e-24
gb|AAF46821.1| (AE003456) CG3264 gene product [Drosophila melano... 111 1e-23
emb|CAC18282.1| (AL451020) alkaline phosphatase [Neurospora crassa] 104 2e-21
pir||T47236 alkaline phosphatase (EC 3.1.3.1) (pho-2+) [imported... 103 4e-21
gb|AAF25669.1| (AF209193) alkaline phosphatase [Schizophyllum co... 102 7e-21
gb|AAD45540.1|U70376_2 (U70376) streptomycin-6-phosphate phospha... 96 7e-19
dbj|BAA88367.1| (AB012643) alkaline phosphatase [Homo sapiens] 77 4e-13
gi|11428788 alkaline phosphatase, placental (Regan isozyme) [Hom... 74 4e-12
>pir||E75081 alkaline phosphatase (EC 3.1.3.1) IV PAB2366 precursor - Pyrococcus
abyssi (strain Orsay) >gi|5458466|emb|CAB49954.1|
(AJ248286) ALKALINE PHOSPHATASE IV PRECURSOR (EC
3.1.3.1) (APASE IV) [Pyrococcus abyssi]
Length = 495
Score = 975 bits (2492), Expect = 0.0
Identities = 495/495 (100%), Positives = 495/495 (100%)
Query: 1 MMKMKLARFLLIFIIFSSMFPFANASPSGVRNVIILIGDGMGFSQLQLTKLVYGHLNMED 60
MMKMKLARFLLIFIIFSSMFPFANASPSGVRNVIILIGDGMGFSQLQLTKLVYGHLNMED
Sbjct: 1 MMKMKLARFLLIFIIFSSMFPFANASPSGVRNVIILIGDGMGFSQLQLTKLVYGHLNMED 60
Query: 61 FPYTGIELTDSLSGEVTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLG 120
FPYTGIELTDSLSGEVTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLG
Sbjct: 61 FPYTGIELTDSLSGEVTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLG 120
Query: 121 KATGLVTTTRITHATPAVFASHVPDRDMEEEIARQLILHNVTVLMGGGREKFSEEVLKLA 180
KATGLVTTTRITHATPAVFASHVPDRDMEEEIARQLILHNVTVLMGGGREKFSEEVLKLA
Sbjct: 121 KATGLVTTTRITHATPAVFASHVPDRDMEEEIARQLILHNVTVLMGGGREKFSEEVLKLA 180
Query: 181 EDYGYSIVYTREDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPN 240
EDYGYSIVYTREDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPN
Sbjct: 181 EDYGYSIVYTREDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPN 240
Query: 241 GFFLMIEGGRIDHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGL 300
GFFLMIEGGRIDHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGL
Sbjct: 241 GFFLMIEGGRIDHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGL 300
Query: 301 GIGLNYGHSVDIDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKN 360
GIGLNYGHSVDIDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKN
Sbjct: 301 GIGLNYGHSVDIDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKN 360
Query: 361 STNKYALGNIIGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAK 420
STNKYALGNIIGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAK
Sbjct: 361 STNKYALGNIIGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAK 420
Query: 421 LMIFGDRSISFTIKGVSKIKGDVTGDYRVDERDAYATLMLLLGDLVDTELENIADMDNNG 480
LMIFGDRSISFTIKGVSKIKGDVTGDYRVDERDAYATLMLLLGDLVDTELENIADMDNNG
Sbjct: 421 LMIFGDRSISFTIKGVSKIKGDVTGDYRVDERDAYATLMLLLGDLVDTELENIADMDNNG 480
Query: 481 IIDLLDVMAILQASS 495
IIDLLDVMAILQASS
Sbjct: 481 IIDLLDVMAILQASS 495
>pir||B72410 alkaline phosphatase - Thermotoga maritima (strain MSB8)
>gi|4980650|gb|AAD35249.1|AE001701_2 (AE001701) alkaline
phosphatase [Thermotoga maritima]
Length = 434
Score = 300 bits (760), Expect = 2e-80
Identities = 181/411 (44%), Positives = 245/411 (59%), Gaps = 28/411 (6%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH-LNMEDFPYTGIELTDSLSGEVTDSAAAGTAI 86
S V+NVI LIGDGMG SQ+ LT ++ G L+ PY G+ T S + VTDSAAAGTA+
Sbjct: 19 SQVKNVIYLIGDGMGLSQVYLTSMLEGRPLSFMKTPYIGLVKTHSANSWVTDSAAAGTAL 78
Query: 87 ATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVFASHVPDR 146
A+G KT N MI N+ + T+ E+A+ G TG+V T R+THATPA F +HV R
Sbjct: 79 ASGFKTNNGMI---NILPDGTVVPTIFEVAKTYGVRTGIVVTCRVTHATPAAFYAHVKSR 135
Query: 147 DMEEEIARQLILHN-VTVLMGGGREKFSEE-----------VLKLAEDYGYSIVYTREDL 194
D E EIARQL+ + V V+MGGG F + +++LA++ GY V TRE+L
Sbjct: 136 DEENEIARQLVENETVDVVMGGGWANFLPKDLGGKRDDNLNLIELAKEKGYIYVKTREEL 195
Query: 195 EKVK--DGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRID 252
K+ K+L LFA HL R E+ + L EM +KA+EIL K+ FFLM+EG +ID
Sbjct: 196 SKISADSDKILALFAPSHLDPASSRKEQPM-LYEMVEKALEILSKDDEPFFLMVEGSQID 254
Query: 253 HACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDI 312
H ND+ + E EFD V LD+A +RGDTLVIV ADHETGGLG+ + + VD+
Sbjct: 255 WEAHDNDIYGVWKEVVEFDKAVQVALDFALKRGDTLVIVTADHETGGLGLS-SGDYRVDV 313
Query: 313 DSIRRIDASIEEMSKEI--KSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNI 370
D IR + + + K + I + GL L+DE++ I +KN K LG I
Sbjct: 314 DKIRNFKKTTDWIMANYSPKDREKFKKAIEEYFGLTLSDEDLNRISMSKNP--KIELGRI 371
Query: 371 IGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKL 421
+GE K+ VG+ + H+G PVP+ A+GPGAENF GF + + ++I KL
Sbjct: 372 LGE----KVSVGWTTTTHSGTPVPIFAFGPGAENFTGFLDNTEIPRIIMKL 418
>sp|P19406|PPB4_BACSU ALKALINE PHOSPHATASE IV PRECURSOR (APASE IV)
>gi|7435092|pir||B69676 alkaline phosphatase (EC
3.1.3.1) IV precursor - Bacillus subtilis
>gi|470383|gb|AAA18323.1| (U02550) alkaline phosphatase
A [Bacillus subtilis] >gi|2226197|emb|CAA74486.1|
(Y14082) alkaline phosphatase [Bacillus subtilis]
>gi|2633276|emb|CAB12780.1| (Z99109) alkaline
phosphatase A [Bacillus subtilis]
Length = 461
Score = 276 bits (698), Expect = 4e-73
Identities = 161/415 (38%), Positives = 237/415 (56%), Gaps = 22/415 (5%)
Query: 30 VRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFP--------YTGIELT--DSLSGEVTDS 79
+RNVI++IGDGMG ++ + + + + + P TG+ +T D +TDS
Sbjct: 49 IRNVIVMIGDGMGTPYIRAYRSMKNNGDTPNNPKLTEFDRNLTGMMMTHPDDPDYNITDS 108
Query: 80 AAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVF 139
AAAGTA+ATGVKTYN I GK V ++LE A+ GK+TGLV T+ I HATPA +
Sbjct: 109 AAAGTALATGVKTYNNAIGVDK-NGKKVK--SVLEEAKQQGKSTGLVATSEINHATPAAY 165
Query: 140 ASHVPDRDMEEEIARQLI------LHNVTVLMGGGREKFSEEVLKLAEDY---GYSIVYT 190
+H R ++IA + H + VL+GGG+ F+ + L +++ GYS V T
Sbjct: 166 GAHNESRKNMDQIANSYMDDKIKGKHKIDVLLGGGKSYFNRKNRNLTKEFKQAGYSYVTT 225
Query: 191 REDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGR 250
++ L+K KD +VLGLFA+G L LDR + SL +MT AI+ L +N GFFLM+EG +
Sbjct: 226 KQALKKNKDQQVLGLFADGGLAKALDRDSKTPSLKDMTVSAIDRLNQNKKGFFLMVEGSQ 285
Query: 251 IDHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSV 310
ID A H ND ++E K+F+ +++A++ TLVI ADH TGG IG N +
Sbjct: 286 IDWAAHDNDTVGAMSEVKDFEQAYKAAIEFAKKDKHTLVIATADHTTGGFTIGANGEKNW 345
Query: 311 DIDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNI 370
+ I + E M+K+I G ++DV+ R+ L++T EE+K +E A +
Sbjct: 346 HAEPILSAKKTPEFMAKKISEGKPVKDVLARYANLKVTSEEIKSVEAAAQADKSKGASKA 405
Query: 371 IGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIFG 425
I +I + + G+ S HTGE VP+ AYGPG E F G ++ D A +I K++ G
Sbjct: 406 IIKIFNTRSNSGWTSTDHTGEEVPVYAYGPGKEKFRGLINNTDQANIIFKILKTG 460
>pir||A39096 alkaline phosphatase (EC 3.1.3.1) IV precursor - Bacillus subtilis
Length = 458
Score = 276 bits (698), Expect = 4e-73
Identities = 161/415 (38%), Positives = 237/415 (56%), Gaps = 22/415 (5%)
Query: 30 VRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFP--------YTGIELT--DSLSGEVTDS 79
+RNVI++IGDGMG ++ + + + + + P TG+ +T D +TDS
Sbjct: 46 IRNVIVMIGDGMGTPYIRAYRSMKNNGDTPNNPKLTEFDRNLTGMMMTHPDDPDYNITDS 105
Query: 80 AAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVF 139
AAAGTA+ATGVKTYN I GK V ++LE A+ GK+TGLV T+ I HATPA +
Sbjct: 106 AAAGTALATGVKTYNNAIGVDK-NGKKVK--SVLEEAKQQGKSTGLVATSEINHATPAAY 162
Query: 140 ASHVPDRDMEEEIARQLI------LHNVTVLMGGGREKFSEEVLKLAEDY---GYSIVYT 190
+H R ++IA + H + VL+GGG+ F+ + L +++ GYS V T
Sbjct: 163 GAHNESRKNMDQIANSYMDDKIKGKHKIDVLLGGGKSYFNRKNRNLTKEFKQAGYSYVTT 222
Query: 191 REDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGR 250
++ L+K KD +VLGLFA+G L LDR + SL +MT AI+ L +N GFFLM+EG +
Sbjct: 223 KQALKKNKDQQVLGLFADGGLAKALDRDSKTPSLKDMTVSAIDRLNQNKKGFFLMVEGSQ 282
Query: 251 IDHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSV 310
ID A H ND ++E K+F+ +++A++ TLVI ADH TGG IG N +
Sbjct: 283 IDWAAHDNDTVGAMSEVKDFEQAYKAAIEFAKKDKHTLVIATADHTTGGFTIGANGEKNW 342
Query: 311 DIDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNI 370
+ I + E M+K+I G ++DV+ R+ L++T EE+K +E A +
Sbjct: 343 HAEPILSAKKTPEFMAKKISEGKPVKDVLARYANLKVTSEEIKSVEAAAQADKSKGASKA 402
Query: 371 IGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIFG 425
I +I + + G+ S HTGE VP+ AYGPG E F G ++ D A +I K++ G
Sbjct: 403 IIKIFNTRSNSGWTSTDHTGEEVPVYAYGPGKEKFRGLINNTDQANIIFKILKTG 457
>dbj|BAB04593.1| (AP001510) alkaline phosphatase [Bacillus halodurans]
Length = 444
Score = 265 bits (670), Expect = 9e-70
Identities = 161/435 (37%), Positives = 254/435 (58%), Gaps = 31/435 (7%)
Query: 9 FLLIFIIFSSMFPFANASPSG--------VRNVIILIGDGMGFSQLQLTKLVYGHLNMED 60
F++I +I + FA+ G V+N+I +I DG S ++ G
Sbjct: 12 FVMIVLILPAETVFASFVQEGTITPDSEQVKNIIYMIPDGYSASYATNYRIYKGEEEPIW 71
Query: 61 FPY-TGIELTDSLSGEVTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQML 119
P+ G+ T S VTDSAAAGTA+ATG KT N T ++ + L ++L+ A
Sbjct: 72 DPHLVGMVKTHSADSWVTDSAAAGTALATGTKTSN---GTIGMSTEGEELESILQAAGKQ 128
Query: 120 GKATGLVTTTRITHATPAVFASHVPDRDMEEEIARQLILHNVTVLMGGGREKF------- 172
K TG+V TTR+THATPA F + VP R E +IA Q+I V VL+GGG++ F
Sbjct: 129 KKGTGIVVTTRLTHATPAAFVASVPLRIDEAKIAPQMI-GQVDVLLGGGKKMFVPEAEGG 187
Query: 173 ---SEEVLKLAEDYGYSIVYTREDLEKVKDGKVLGLFAEGHLPYVLDRS-EEDVSLLEMT 228
S +L AED GY+++ T+E+L+ V KVLGLF+EG L LDR ++ SL EMT
Sbjct: 188 MQNSRHLLNEAEDAGYALIETKEELQNVNSAKVLGLFSEGALAPELDRQGTQEPSLAEMT 247
Query: 229 KKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTL 288
+ A+ L++NPNGFFLM+EG +ID A H ND A + + + F++ V D+A + G+TL
Sbjct: 248 EAALHTLKQNPNGFFLMVEGSQIDWAGHDNDAAWAMKDIEAFEEAVSVAFDFAEKDGNTL 307
Query: 289 VIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMSKEI-KSGGDIRDVIRRHTGLEL 347
V+V+ DH+TGGL +G + D ++++ A+ + ++ ++ K ++ DV++ +TG EL
Sbjct: 308 VVVVGDHDTGGLSVGAYGQYKAKPDMLKQVKATGKFIASQVNKRRTNLADVMKTYTGFEL 367
Query: 348 TDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVG 407
T +E+K+I++A + T N I ++IS++ +G+ + HTG +P+ A+GP ++ G
Sbjct: 368 TKKELKQIKKADHLT------NAINQVISRRALIGWTTPVHTGTDIPIYAFGPCSDRLHG 421
Query: 408 FKHHVDTAKVIAKLM 422
+ D ++A +M
Sbjct: 422 LLDNTDIPLILADVM 436
>gb|AAG33874.1|AF320303_1 (AF320303) alkaline phosphatase [Lactobacillus delbrueckii subsp.
bulgaricus]
Length = 471
Score = 261 bits (661), Expect = 1e-68
Identities = 153/423 (36%), Positives = 239/423 (56%), Gaps = 25/423 (5%)
Query: 24 NASPSGVRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFPYTGIELTDSLSG--------- 74
N S + ++NVI+LIGDGMG S + + Y N + F E L G
Sbjct: 48 NGSNAKIKNVIVLIGDGMGPSYMTAHR--YMKDNPKTFEMESTEFDKHLVGTQKTYPEDE 105
Query: 75 --EVTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRIT 132
+TDSA+A TA++ G+KTYN I+ N ++ T+LE A+ GK+TGLV T+ IT
Sbjct: 106 HENITDSASAATAMSAGIKTYNAAIAVDNDKAEV---KTVLEQAKEKGKSTGLVATSEIT 162
Query: 133 HATPAVFASHVPDRDMEEEIARQLI------LHNVTVLMGGGREKFSEEVLKLAEDY--- 183
HATPA F +H R + IA H V V++GGG + F + L E++
Sbjct: 163 HATPAAFGAHDISRKNMDAIANDYFDEKIKGKHKVDVMLGGGVKNFVRKDRNLTEEFKKS 222
Query: 184 GYSIVYTREDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFF 243
GYS V R+ L K+ ++LGLFA G L ++DR+E+ SL EMT AIE L KN NGFF
Sbjct: 223 GYSYVTDRDQLLNDKNDQILGLFAPGGLDKMIDRNEKTPSLEEMTNAAIERLNKNKNGFF 282
Query: 244 LMIEGGRIDHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIG 303
LM+EG +ID A H ND+ ++E ++F+ +++A++ +TLV+ ADH TGGL +G
Sbjct: 283 LMVEGSQIDWAGHDNDIVGAMSEMEDFEKAFKAAIEFAKKDKNTLVVATADHATGGLSLG 342
Query: 304 LNYGHSVDIDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTN 363
N ++ ++ I+ + + M+ EI G ++ + ++++ L+LT EE++ + + S +
Sbjct: 343 ANGEYNFKVEPIKAAKRTPDFMANEIAKGANVEETLKKYIDLQLTPEEIQAVNDIAPSKD 402
Query: 364 KYALGNIIGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMI 423
+ N I +I +K+ G+ + HTGE V + A+GPG F G + + + AK + ++
Sbjct: 403 VTKIDNAIEDIFNKRSVTGWTTGGHTGEDVNVYAFGPGKYLFSGVQENTNIAKRVFDIVG 462
Query: 424 FGD 426
GD
Sbjct: 463 GGD 465
>pir||C69676 alkaline phosphatase (EC 3.1.3.1) III precursor [validated] -
Bacillus subtilis >gi|1945090|dbj|BAA19698.1| (D88802)
B. subtilis alkaline phosphatase IIIA; P19405 secretory
[Bacillus subtilis] >gi|2632887|emb|CAB12393.1| (Z99107)
alkaline phosphatase III [Bacillus subtilis]
Length = 462
Score = 255 bits (645), Expect = 7e-67
Identities = 152/414 (36%), Positives = 228/414 (54%), Gaps = 24/414 (5%)
Query: 30 VRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFPYTGIE---------LTDSLSGEVTDSA 80
++NVI+LIGDGMG S + + + + T + D VTDSA
Sbjct: 43 IKNVIVLIGDGMGVSYTSAYRYLKDNKKTKVVEPTAFDQYLVGQQTTYPDDPEQNVTDSA 102
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVFA 140
AA TA++ G+KTYN I+ N + T+LE A+ GKATGLV T+ ITHATPA F
Sbjct: 103 AAATAMSAGIKTYNNAIAVDNDGSEA---KTVLEAAKEKGKATGLVATSEITHATPASFG 159
Query: 141 SHVPDRDMEEEIARQLI------LHNVTVLMGGGREKFSEEVLKLAEDY---GYSIVYTR 191
SH R IA H + VL+GGG+ F + L +++ GYS V R
Sbjct: 160 SHDHSRKNMNSIADDYFDEMVNGKHKIDVLLGGGKSNFDRKDRNLIKEFKKAGYSYVDDR 219
Query: 192 EDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRI 251
+D+ K KD +VLGLFA+G LP +DR+++ SL +MT AI+ L K+ +GFFLM+EG +I
Sbjct: 220 KDMLKNKDSQVLGLFADGGLPKKIDRTKDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQI 279
Query: 252 DHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVD 311
D A H ND+ ++E ++F+ +D+A++ TLV+ ADH TGG IG + ++
Sbjct: 280 DWAGHDNDIVGAMSEMEDFEQAYKAAIDFAKKDKHTLVVATADHSTGGYSIGADSIYNWF 339
Query: 312 IDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTG---LELTDEEVKEIEEAKNSTNKYALG 368
+ I+ + + M+++I G D+ ++ + L LT E++ +EEA S +
Sbjct: 340 SEPIKAAKRTPDFMAEKIADGADVEKTLKTYIDQKKLALTKAEIQSVEEAAKSKEVLDID 399
Query: 369 NIIGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLM 422
N I I +K+ G+ + HTGE VP+ AYGP +E F G + + AK + K +
Sbjct: 400 NAIENIFNKRSHTGWTTGGHTGEDVPVYAYGPSSETFAGQIDNTEIAKNVFKAL 453
>gb|AAD38069.1|AF154110_1 (AF154110) alkaline phosphatase [Enterococcus faecalis]
Length = 471
Score = 254 bits (642), Expect = 2e-66
Identities = 150/427 (35%), Positives = 235/427 (54%), Gaps = 21/427 (4%)
Query: 30 VRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFPYTGIEL---------TDSLSGEVTDSA 80
V+NVI +IGDGMG + + + + P T + + VTDSA
Sbjct: 48 VKNVIFMIGDGMGNPYTTGYRYFKANHSDKRVPQTAFDTYLVGQQATYPEDEEENVTDSA 107
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVFA 140
+A TA+A GVKTYN I+ N K T+LE A+ +GK+TGLV T+ ITHATPA +
Sbjct: 108 SAATAMAAGVKTYNNAIALDNDKSKT---ETVLERAKKVGKSTGLVATSEITHATPAAYG 164
Query: 141 SHVPDRDMEEEIARQLI------LHNVTVLMGGGREKFSEEVLKLAEDY---GYSIVYTR 191
+H R EIA H V VL+GGG E F+ + L +++ GY V +
Sbjct: 165 AHNVSRKNMAEIADDYFDDQIDGQHKVDVLLGGGSELFARKDRDLVKEFSQAGYGHVTDK 224
Query: 192 EDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRI 251
+ L + +D K+LGLFA G LP ++DR+EE SL +MT+ A++ L+KN GFFLM+EG +I
Sbjct: 225 KSLNENQDDKILGLFAPGGLPKMIDRTEEVPSLADMTEAALQRLDKNEKGFFLMVEGSQI 284
Query: 252 DHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVD 311
D A H+ND+ ++E ++F+ +D+A++ G+TLV+ ADH TGGL +G ++
Sbjct: 285 DWAGHSNDIVGAMSEMQDFEAAFEKAIDFAKKDGETLVVTTADHSTGGLSLGKGDQYNWL 344
Query: 312 IDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNII 371
+ + + + M++EI G++ + + +L++ E+K ++ A S + + +
Sbjct: 345 TEPLHAAKRTPDFMAEEIIKNGNVEKTVTEYIDFQLSEAELKAVKTAAESKDVEKIAQAL 404
Query: 372 GEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIFGDRSISF 431
+I ++ G+ + HTGE V + AYGP AE F G + D AK+I L+ +
Sbjct: 405 RKIFDERSNTGWTTGGHTGEDVNVYAYGPQAEAFSGQIDNTDQAKIIFGLVDGTGQKAEI 464
Query: 432 TIKGVSK 438
KG+ K
Sbjct: 465 KDKGIGK 471
>gb|AAD53269.1|AF167172_1 (AF167172) alkaline phosphatase [Cloning vector pDC123]
Length = 498
Score = 254 bits (642), Expect = 2e-66
Identities = 150/427 (35%), Positives = 235/427 (54%), Gaps = 21/427 (4%)
Query: 30 VRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFPYTGIEL---------TDSLSGEVTDSA 80
V+NVI +IGDGMG + + + + P T + + VTDSA
Sbjct: 75 VKNVIFMIGDGMGNPYTTGYRYFKANHSDKRVPQTAFDTYLVGQQATYPEDEEENVTDSA 134
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVFA 140
+A TA+A GVKTYN I+ N K T+LE A+ +GK+TGLV T+ ITHATPA +
Sbjct: 135 SAATAMAAGVKTYNNAIALDNDKSKT---ETVLERAKKVGKSTGLVATSEITHATPAAYG 191
Query: 141 SHVPDRDMEEEIARQLI------LHNVTVLMGGGREKFSEEVLKLAEDY---GYSIVYTR 191
+H R EIA H V VL+GGG E F+ + L +++ GY V +
Sbjct: 192 AHNVSRKNMAEIADDYFDDQIDGQHKVDVLLGGGSELFARKDRDLVKEFSQAGYGHVTDK 251
Query: 192 EDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRI 251
+ L + +D K+LGLFA G LP ++DR+EE SL +MT+ A++ L+KN GFFLM+EG +I
Sbjct: 252 KSLNENQDDKILGLFAPGGLPKMIDRTEEVPSLADMTEAALQRLDKNEKGFFLMVEGSQI 311
Query: 252 DHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVD 311
D A H+ND+ ++E ++F+ +D+A++ G+TLV+ ADH TGGL +G ++
Sbjct: 312 DWAGHSNDIVGAMSEMQDFEAAFEKAIDFAKKDGETLVVTTADHSTGGLSLGKGDQYNWL 371
Query: 312 IDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNII 371
+ + + + M++EI G++ + + +L++ E+K ++ A S + + +
Sbjct: 372 TEPLHAAKRTPDFMAEEIIKNGNVEKTVTEYIDFQLSEAELKAVKTAAESKDVEKIAQAL 431
Query: 372 GEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIFGDRSISF 431
+I ++ G+ + HTGE V + AYGP AE F G + D AK+I L+ +
Sbjct: 432 RKIFDERSNTGWTTGGHTGEDVNVYAYGPQAEAFSGQIDNTDQAKIIFGLVDGTGQKAEI 491
Query: 432 TIKGVSK 438
KG+ K
Sbjct: 492 KDKGIGK 498
>sp|P19405|PPB3_BACSU ALKALINE PHOSPHATASE III PRECURSOR (APASE III)
>gi|98234|pir||B39096 alkaline phosphatase (EC 3.1.3.1)
III precursor - Bacillus subtilis
Length = 462
Score = 250 bits (632), Expect = 2e-65
Identities = 150/414 (36%), Positives = 226/414 (54%), Gaps = 24/414 (5%)
Query: 30 VRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFPYTGIE---------LTDSLSGEVTDSA 80
++NVI+LIGDGMG S + + + + T + D VTDSA
Sbjct: 43 IKNVIVLIGDGMGVSYTSAYRYLKDNKKTKVVEPTAFDQYLVGQQTTYPDDPEQNVTDSA 102
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVFA 140
AA TA++ G+KTYN I+ N + T+LE A+ GKATGLV T+ ITHATPA F
Sbjct: 103 AAATAMSAGIKTYNNAIAVDNDGSEA---KTVLEAAKEKGKATGLVATSEITHATPASFG 159
Query: 141 SHVPDRDMEEEIARQLI------LHNVTVLMGGGREKFSEEVLKLAEDY---GYSIVYTR 191
SH R IA H + VL+GGG+ F + L +++ GYS V R
Sbjct: 160 SHDHSRKNMNSIADDYFDEMVNGKHKIDVLLGGGKSNFDRKDRNLIKEFKKAGYSSVDDR 219
Query: 192 EDLEKVKDGKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRI 251
+D+ K KD +VLGL +G LP +DR+++ SL +MT AI+ L K+ +GFFLM+EG +I
Sbjct: 220 KDMLKNKDSQVLGLLPDGGLPKKIDRTKDIPSLKDMTNTAIKKLNKDKDGFFLMVEGSQI 279
Query: 252 DHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVD 311
D A H ND+ ++E ++F+ +D+A++ TLV+ ADH TGG IG + ++
Sbjct: 280 DWAGHDNDIVGAMSEMEDFEQAYKAAIDFAKKDKHTLVVATADHSTGGYSIGADGIYNWF 339
Query: 312 IDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTG---LELTDEEVKEIEEAKNSTNKYALG 368
+ I+ + + M+++I G D+ ++ + L LT E++ +EEA S +
Sbjct: 340 SEPIKAAKRTPDFMAEKIADGADVEKTLKTYIDQKKLALTKAEIQSVEEAAKSKEVLDID 399
Query: 369 NIIGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLM 422
N I I +K+ G+ + HTGE VP+ AYGP +E F G + + AK + K +
Sbjct: 400 NAIENIFNKRSHTGWTTGGHTGEDVPVYAYGPSSETFAGQIDNTEIAKNVFKAL 453
>gb|AAG20936.1| (AE005160) alkaline phosphatase; Aph [Halobacterium sp. NRC-1]
Length = 473
Score = 217 bits (547), Expect = 2e-55
Identities = 153/437 (35%), Positives = 223/437 (51%), Gaps = 50/437 (11%)
Query: 23 ANASPSGVRNVIILIGDGMGFSQLQLTKLVYGHLNM-EDFPY--------TGIELTDSLS 73
A A+ S N I I DGMG +Q+ + + + E FP TG + S
Sbjct: 40 AQATASPAANAIAYIVDGMGQTQISAARYLNAYKTAPERFPLNVSPAETPTGFDAFSSRG 99
Query: 74 G----------EVTDSAAAGTAIATGVKTYNRMISTTNVTGK-LVNLTTLLEIAQMLGKA 122
TDSAAA TA A+GVKTYN I +G + T+LE A G A
Sbjct: 100 SMTTFPDDPYETTTDSAAAATAFASGVKTYNGAIGGVQTSGGGFQRVDTVLERASAQGYA 159
Query: 123 TGLVTTTRITHATPAVFASHVPDRDMEEEIARQLILH-NVTVLMGGGREKFSEE------ 175
TGL+TTT THATPA FA+HV DR + EIARQ I V++GG R F +
Sbjct: 160 TGLITTTEATHATPAAFAAHVEDRGNQTEIARQYIEETQPDVILGGQRRDFEADASNGGT 219
Query: 176 VLKLAEDYGYSIVYTREDLEKVKDGKVLGLFA-EGHLPYVLDRSEE----DVSLLEMTKK 230
++ A D GY+I T +L+ V D VLGLF+ E HL Y LDR + +L M
Sbjct: 220 LVDAARDNGYTIAETAAELDAVDDPPVLGLFSQESHLDYYLDRKNDPENTQPNLDAMVDA 279
Query: 231 AIEILEK--NPN-GFFLMIEGGRIDHACHANDVASIVAETKEFDDVVGYVLDYARRRGD- 286
+++L +P+ GFFL++E GR+DHA HAN A VAE E V G +++YA +
Sbjct: 280 GVDLLSSAGDPDKGFFLLVESGRVDHAGHANYPAQ-VAEQYEATQVAGQLVEYAETTAEP 338
Query: 287 TLVIVLADHETGGLGIGLNYGHSVDIDSI---RRIDASIEEMSKEIKSGGDIRDVIRRHT 343
T ++ DHE GGL +G + + V+ D + + + + ++ ++S ++ ++ HT
Sbjct: 339 TFLVSTGDHECGGLTLGRDSPYEVEYDVLAAQKATTSRLRDLLAGVRSADELESIVAAHT 398
Query: 344 GL-ELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSHKHTGEPVPLLAYGPGA 402
G+ LTD EV ++ +A S I I++++ G+ F + HTG VP+ A+GP A
Sbjct: 399 GITALTDREVAKLRDAPGS---------ISTILAERAGIAFTTDGHTGTDVPVFAHGPNA 449
Query: 403 ENFVGFKHHVDTAKVIA 419
F + + A +A
Sbjct: 450 ARFDAARDNTAVADALA 466
>gb|AAG10093.1|U79570_2 (U79570) alkaline phosphatase I [Bacillus licheniformis]
Length = 553
Score = 207 bits (521), Expect = 3e-52
Identities = 139/429 (32%), Positives = 221/429 (51%), Gaps = 38/429 (8%)
Query: 30 VRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFPYTGIELTDSLSGEVTDSAAAGTAIATG 89
V+NVI+++ DG S L + G D +G T S +TDSA A TA+ATG
Sbjct: 40 VKNVIMMVMDGTSSSATTLARWYKGEPLAMDQIVSGGVRTYSAESAITDSAPAATAMATG 99
Query: 90 VKTYNRMI----STTNVTG--------KLVNLTTLLEIAQMLGKATGLVTTTRITHATPA 137
K+ + I S N G + +LE A+ GKATG++ T+ I HATPA
Sbjct: 100 RKSNSSYIGVLPSKVNTPGVEKVREKDHYRPVANVLEGAKQKGKATGIIATSEIQHATPA 159
Query: 138 VFASHVPDRDMEEEIARQLILHNVTVLMGGGRE-----------KFSEEVLKLAEDYGYS 186
++ H DR+ E + Q + N+ V++GGG+E K E+++K+ +D Y
Sbjct: 160 GYSGHHKDRNNFEVLGEQQVYQNMDVVLGGGKESLQPGTKDKSRKDGEDLVKVLKDKKYD 219
Query: 187 IVYTREDLEKVKDGKVLGLFAEGHLPYVLDRSE---EDVSLLEMTKKAIEILEKNPNGFF 243
V T++ L K K K+ G F++ L Y +DR E ++ EMTKK+I+ L K+ +GFF
Sbjct: 220 FVETKDQLLKSKSKKIWGAFSQRDLAYDMDRRATHPEQPTIAEMTKKSIQTLSKDKDGFF 279
Query: 244 LMIEGGRIDHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIG 303
L +EG + D A H ND ++++ FD+ V LD+A++ T+VI ++DH G+ IG
Sbjct: 280 LFVEGSKPDWAAHQNDPIGMISDVLAFDNAVKEALDFAKKDKHTMVIAVSDHGNSGISIG 339
Query: 304 ---LNYGH-----SVDIDSIRRIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEI 355
G+ S +D +++ ++E + ++K D+ ++ L D E
Sbjct: 340 NSNTTKGYDTTPVSAYVDPLKKAKMTVEGATGKLKD--DLSNIEEAAKLYGLDDLTPAEK 397
Query: 356 EEAKNSTNKYALGNIIGEIISKKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTA 415
E+ K+ NK ++ +++S + +GF + HTGE V L +YGP E G + D A
Sbjct: 398 EQLKSVKNKKEAASLFTKLLSNRANIGFTTGGHTGEDVFLYSYGP--EKPQGLVENTDIA 455
Query: 416 KVIAKLMIF 424
K +AK M F
Sbjct: 456 KTMAKAMGF 464
>gb|AAC31201.1| (AF079878) alkaline phosphatase [Thermus sp. FD3041]
Length = 501
Score = 182 bits (457), Expect = 8e-45
Identities = 136/418 (32%), Positives = 205/418 (48%), Gaps = 57/418 (13%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN---------MEDFPYTGIELTDSLSGEVTDSAA 81
RN+I+ + DG + + + + +P G+ T SL+ VT+S+A
Sbjct: 39 RNLIVFVYDGFSWEDYAIAQAYARRRQGRVLALERLLARYP-NGLINTYSLTSYVTESSA 97
Query: 82 AGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVFAS 141
AG A + GVKT N ++ + L A+ GKA GLVTTT +THATPA F
Sbjct: 98 AGNAFSCGVKTVNGGLA---IHADGTPLKPFFAAAKEAGKAVGLVTTTTVTHATPASFVV 154
Query: 142 HVPDRDMEEEIARQLILHNVTVLMGGGREKFSEEVLKLAEDY-------GYSIVYTREDL 194
PDR+ EE IA Q + V +GGG F+ K +D GY +V T E+L
Sbjct: 155 SNPDRNAEERIAEQYLEFGAEVYLGGGDRFFNPARRKDGKDLYAAFAAKGYGVVRTPEEL 214
Query: 195 EKVKDGKVLGLFAEGHLPYVLDRSEEDV---SLLEMTKKAIEILEKNPNGFFLMIEGGRI 251
+ ++LG+FA+GH+PY +DR + + SL EM + A+ L + GF L +E GRI
Sbjct: 215 ARSNATRLLGVFADGHVPYEIDRRFQGLGVPSLKEMVQAALPRLAAHRGGFVLQVEAGRI 274
Query: 252 DHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYG---- 307
DHA H ND + + + D+V+ + + R DTL++V++DH T G+G YG
Sbjct: 275 DHANHLNDAGATLWDVLAADEVLELLTAFVDRNPDTLLLVVSDHAT---GVGALYGAGRS 331
Query: 308 ---HSVDIDSIRRIDASIEEMSKEIKSGGD---IRDVIRRHTGLELTDEEVKEIEEA--- 358
SV ID + AS E M + + S D +++ + G+ LTDEE + + A
Sbjct: 332 YLESSVGIDLLGAQKASFEYMRRVLGSAPDAAQVKEAYQTLKGVSLTDEEAQMVVRAIRE 391
Query: 359 -------------KNSTNKYALGNIIGEIISK--KLGVGFVSHKHTGEPVPLLAYGPG 401
+T +A+ + + SK + +G+ S +HT PV LL YG G
Sbjct: 392 RVYWPDAVRQGIQPENTMAWAM---VQKNASKPDRPNIGWSSGQHTASPVILLLYGQG 446
>gb|AAF13361.1|AF168770_1 (AF168770) alkaline phosphatase [Thermus caldophilus]
Length = 501
Score = 178 bits (446), Expect = 2e-43
Identities = 132/412 (32%), Positives = 198/412 (48%), Gaps = 45/412 (10%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN---------MEDFPYTGIELTDSLSGEVTDSAA 81
RN+I+ + DG + + + + +P G+ T SL+ VT+S+A
Sbjct: 39 RNLIVFVYDGFSWEDYAIAQAYARRRQGRVLALERLLARYP-NGLINTYSLTSYVTESSA 97
Query: 82 AGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVFAS 141
AG A + GVKT N ++ + L A+ GKA GLVTTT +THATPA F
Sbjct: 98 AGNAFSCGVKTVNGGLA---IHADGTPLKPFFAAAKEAGKAVGLVTTTTVTHATPASFVI 154
Query: 142 HVPDRDMEEEIARQLILHNVTVLMGGGREKFSEEVLKLAEDY-------GYSIVYTREDL 194
PDR+ EE IA Q + V +GGG F+ K +D GY +V T E+L
Sbjct: 155 SNPDRNAEERIAEQYLEFGAEVYLGGGDRFFNPARRKDGKDLYAAFAAKGYGVVRTPEEL 214
Query: 195 EKVKDGKVLGLFAEGHLPYVLDRSEEDV---SLLEMTKKAIEILEKNPNGFFLMIEGGRI 251
+ ++LG+FA+GH+PY +DR + + SL EM + A+ L + GF L +E GRI
Sbjct: 215 ARSNATRLLGVFADGHVPYEIDRRFQGLGVPSLKEMVQAALPRLAAHRGGFVLQVEAGRI 274
Query: 252 DHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETG--GL-GIGLNYGH 308
DHA H ND + + + D+V+ + + R DTL+IV++DH TG GL G G +Y
Sbjct: 275 DHANHLNDAGATLWDVLAADEVLELLTAFVDRNPDTLLIVVSDHATGVGGLYGAGRSYLE 334
Query: 309 SVD-IDSIRRIDASIEEMSK---EIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNK 364
S +D + AS E M + + +++ R G++L D E + + A
Sbjct: 335 SSQGVDLLEPQRASFEHMLRVLGQAPEASQVKEAFRAMKGVDLEDAEAERVVRAIREKVY 394
Query: 365 YALGNIIGEIISKKLG---------------VGFVSHKHTGEPVPLLAYGPG 401
+ G G + + +G+ S +HT PV LL YG G
Sbjct: 395 WPEGVRQGVQPANTMAWAMVQRDAKKPDRPNIGWSSGQHTASPVMLLLYGQG 446
>gi|6320689 repressible alkaline phosphatase; Pho8p [Saccharomyces cerevisiae]
>gi|2851586|sp|P11491|PPB_YEAST REPRESSIBLE ALKALINE
PHOSPHATASE PRECURSOR >gi|2117980|pir||S69648 alkaline
phosphatase (EC 3.1.3.1) - yeast (Saccharomyces
cerevisiae) >gi|927758|gb|AAB64930.1|AAB64930 (U33050)
Pho8p: repressible alkaline phosphatase; CAI: 0.16
[Saccharomyces cerevisiae]
Length = 566
Score = 175 bits (439), Expect = 1e-42
Identities = 143/456 (31%), Positives = 218/456 (47%), Gaps = 75/456 (16%)
Query: 10 LLIFIIFSSMFPFANASPSGVRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFP------- 62
+L+ + F S F +AS +NVI + DGMG + L + + H+N D P
Sbjct: 47 VLVQLAFPSSFALRSASHKK-KNVIFFVTDGMGPASLSMARSFNQHVN--DLPIDDILTL 103
Query: 63 ---YTGIELTDSLSGEVTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQML 119
+ G T S VTDSAA TA A +K+YN I V T+LE A++
Sbjct: 104 DEHFIGSSRTRSSDSLVTDSAAGATAFACALKSYNGAIG---VDPHHRPCGTVLEAAKLA 160
Query: 120 GKATGLVTTTRITHATPAVFASHVPDRDMEEEIARQLILHN-----VTVLMGGGREKF-- 172
G TGLV TTRIT ATPA F+SHV R E+ IA + V +LMGGGR F
Sbjct: 161 GYLTGLVVTTRITDATPASFSSHVDYRWQEDLIATHQLGEYPLGRVVDLLMGGGRSHFYP 220
Query: 173 ----------------SEEVLKLAEDYGYSIVYTREDLEKVKDGK-------VLGLFAEG 209
+++ A+ G+ V R++ + + LGLFA+
Sbjct: 221 QGEKASPYGHHGARKDGRDLIDEAQSNGWQYVGDRKNFDSLLKSHGENVTLPFLGLFADN 280
Query: 210 HLPYVLDRSEEDV-SLLEMTKKAIEILEK------NPNGFFLMIEGGRIDHACHANDVAS 262
+P+ +DR E++ SL E K A+ LEK + NGFFLM+EG RIDHA H ND AS
Sbjct: 281 DIPFEIDRDEKEYPSLKEQVKVALGALEKASNEDKDSNGFFLMVEGSRIDHAGHQNDPAS 340
Query: 263 IVAETKEFDDVVGYVLDYARRRG-DTLVIVLADHETGGL-----------------GIGL 304
V E FD+ YVL++A +T+++ +DHETGGL +
Sbjct: 341 QVREVLAFDEAFQYVLEFAENSDTETVLVSTSDHETGGLVTSRQVTASYPQYVWYPQVLA 400
Query: 305 NYGHSVDIDSIRRIDASIEEMSKEIKSGGDIR-DVIRRHTGL-ELTDEEVKEIEEAKNST 362
N HS + + +D E K I+ +++ + G+ + TD +++ + ++
Sbjct: 401 NATHSGEFLKRKLVDFVHEHKGASSKIENFIKHEILEKDLGIYDYTDSDLETLIHLDDNA 460
Query: 363 NKYALGNIIGEIISKKLGVGFVSHKHTGEPVPLLAY 398
N A+ + + +++S + +G+ +H H+ V + AY
Sbjct: 461 N--AIQDKLNDMVSFRAQIGWTTHGHSAVDVNIYAY 494
>gb|AAA34871.1| (M21134) repressible alkaline phosphatase (EC 3.1.3.1)
[Saccharomyces cerevisiae]
Length = 566
Score = 173 bits (434), Expect = 4e-42
Identities = 141/456 (30%), Positives = 219/456 (47%), Gaps = 75/456 (16%)
Query: 10 LLIFIIFSSMFPFANASPSGVRNVIILIGDGMGFSQLQLTKLVYGHLNMEDFP------- 62
+L+ + F + F +AS +NVI + DGMG + L + + H+N D P
Sbjct: 47 VLVQLAFPTSFAIRSASHKK-KNVIFFVTDGMGPASLSMARSFNQHVN--DLPIDDILTL 103
Query: 63 ---YTGIELTDSLSGEVTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQML 119
+ G T S VTDSAA TA A+ +K+YN I V T+LE A++
Sbjct: 104 DEHFIGSSRTRSSDSLVTDSAAGATAFASALKSYNGAIG---VDPHHRPCGTVLEAAKLA 160
Query: 120 GKATGLVTTTRITHATPAVFASHVPDRDMEEEIARQLILHN-----VTVLMGGGREKF-- 172
G TGLV TTRIT ATPA F+SHV R E+ IA + V +LMGGGR F
Sbjct: 161 GYLTGLVVTTRITDATPASFSSHVDYRWQEDLIATHQLGEYPLGRVVDLLMGGGRSHFYP 220
Query: 173 ----------------SEEVLKLAEDYGYSIVYTREDLEKVKDGK-------VLGLFAEG 209
+++ A+ G+ V R++ + + LGLFA+
Sbjct: 221 QGEKASPYGHHGARKDGRDLIDEAQSNGWQYVGDRKNFDSLLKSHGENVTFPFLGLFADN 280
Query: 210 HLPYVLDRSEEDV-SLLEMTKKAIEILEK------NPNGFFLMIEGGRIDHACHANDVAS 262
+P+ +DR E++ SL E K A+ LEK + NGFFLM+EG RIDHA H ND AS
Sbjct: 281 DIPFEIDRDEKEYPSLKEQVKVALGALEKASNEDKDSNGFFLMVEGSRIDHAGHQNDPAS 340
Query: 263 IVAETKEFDDVVGYVLDYARRRG-DTLVIVLADHETGGL-----------------GIGL 304
V E FD+ YVL++A +T+++ +DHETGGL +
Sbjct: 341 QVREVLAFDEAFQYVLEFAENSDTETVLVSTSDHETGGLVTSRQVTASYPQYVWYPQVLA 400
Query: 305 NYGHSVDIDSIRRIDASIEEMSKEIKSGGDIR-DVIRRHTGL-ELTDEEVKEIEEAKNST 362
N HS + + +D E K I+ +++ + G+ + T+ +++ + ++
Sbjct: 401 NATHSGEFLKRKLVDFVHEHKGASSKIENFIKHEILEKDLGIYDYTESDLETLIHLDDNA 460
Query: 363 NKYALGNIIGEIISKKLGVGFVSHKHTGEPVPLLAY 398
N A+ + + +++S + +G+ +H H+ V + AY
Sbjct: 461 N--AIQDKLNDMVSFRAQIGWTTHGHSAVDVNIYAY 494
>emb|CAB82508.1| (Y18016) alkaline phosphatase [Antarctic bacterium TAB5]
Length = 375
Score = 159 bits (398), Expect = 7e-38
Identities = 98/276 (35%), Positives = 150/276 (53%), Gaps = 11/276 (3%)
Query: 31 RNVIILIGDGMGFSQLQLT-KLVYGHLNMEDFPYTGIELTDSLSGEVTDSAAAGTAIATG 89
+NVI+LI DG G SQ+ T G N F G+ T S +VTDSA+ TA + G
Sbjct: 35 KNVILLISDGAGLSQISSTFYFKEGTPNYTQFKNIGLIKTSSSREDVTDSASGATAFSCG 94
Query: 90 VKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVFASHVPDRDME 149
+KTYN I V + +++EIA + TG+V T+ ITHATPA F +H +R +E
Sbjct: 95 IKTYNAAIG---VADDSTAVKSIVEIAALNNIKTGVVATSSITHATPASFYAHALNRGLE 151
Query: 150 EEIARQLILHNVTVLMGGG-----REKFSEEVLKLAEDYGYSIVYTR-EDLEKVKDGKVL 203
EEIA + ++ GGG + K ++VL + + ++I T D + + +
Sbjct: 152 EEIAMDMTESDLDFFAGGGLNYFTKRKDKKDVLAILKGNQFTINTTGLTDFSSIASNRKM 211
Query: 204 GLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASI 263
G +++ + L T AI+ L K+ + FF+M EG +ID HAN+ + +
Sbjct: 212 GFLLADEAMPTMEKGRGNF-LSAATDLAIQFLSKDNSAFFIMSEGSQIDWGGHANNASYL 270
Query: 264 VAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGG 299
++E +FDD +G L +A++ G+TLVIV +DHETGG
Sbjct: 271 ISEINDFDDAIGTALAFAKKDGNTLVIVTSDHETGG 306
>gb|AAF48480.1| (AE003499) CG8105 gene product [Drosophila melanogaster]
Length = 483
Score = 158 bits (396), Expect = 1e-37
Identities = 108/293 (36%), Positives = 161/293 (54%), Gaps = 32/293 (10%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVTDSA 80
+NV++L+GDG+ + L +++ G L +E FP++G+ T + + DSA
Sbjct: 68 KNVVMLLGDGLSITTLTAARILKGQRRGGRGEDAQLAVEQFPFSGLSKTYCIDEQTPDSA 127
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVFA 140
TA GVKT++ T +G + ++L+ AQ GKATG+VTTTR+T A+PA
Sbjct: 128 CTATAYFGGVKTHS---GTVGQSGSGERVDSVLQWAQRAGKATGVVTTTRLTDASPAGAY 184
Query: 141 SHVPDRDMEEEIARQLIL----HNVTVLMGGGREKFSEE------VLKLAEDYGYSIVYT 190
+HV R E EIARQL+ N+ V++GGG KF++E +L + + ++
Sbjct: 185 AHVSRRGEELEIARQLVEEEPGRNLNVILGGGLGKFADERRDGKDLLAQWRESNPNGCFS 244
Query: 191 R--EDLEKVKD--GKVLGLFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEK----NPNGF 242
R ++L D G +LG+F++ H+ Y L S+E L +M AIE LE+ + NG+
Sbjct: 245 RSLKELRNCTDATGTLLGIFSDSHMSYHLAASKELPRLCDMAAAAIEKLERKSQEDGNGY 304
Query: 243 FLMIEGGRIDHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
F+ IEGGRIDH H V + E EFD V V+ R DTLV+V ADH
Sbjct: 305 FVFIEGGRIDHGHHETRVGYALDEMVEFDAAVETVIQMTDPR-DTLVVVTADH 356
>pir||H82777 alkaline phosphatase XF0657 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9105535|gb|AAF83467.1|AE003910_3
(AE003910) alkaline phosphatase [Xylella fastidiosa]
Length = 576
Score = 150 bits (374), Expect = 4e-35
Identities = 113/336 (33%), Positives = 158/336 (46%), Gaps = 67/336 (19%)
Query: 23 ANASPSG-VRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDS 71
AN + +G +NVI+ +GDGM F+ + +++ G L+ E FP T T +
Sbjct: 69 ANGAMAGKAKNVILFLGDGMSFTTVAAARILEGQRNAATGEENVLSWEHFPATAFSKTYN 128
Query: 72 LSGEVTDSAAAGTAIATGVKTYNRMISTT--------NVTGKLVNLTTLLEIAQMLGKAT 123
+ DSA A TAI +GVKT+ I + + GK L T L +A G AT
Sbjct: 129 TDAQTADSAGAMTAITSGVKTHMGAIGVSAGQRNDCVDSLGK--GLLTWLTLADSAGMAT 186
Query: 124 GLVTTTRITHATPAVFASHVPDRDMEEE--------------IARQLIL-----HNVTVL 164
G++TTTRITHATPA +H P+R E + IA+QL+ V+
Sbjct: 187 GIITTTRITHATPAALYAHTPERHWESDANLPEAAKAGGCRDIAQQLLTSTRYGRGPLVV 246
Query: 165 MGGGREKFSE----------------------EVLKLAEDYGYSIVYTREDLEKVKDGKV 202
+GGGR +F E K A G + T + +
Sbjct: 247 LGGGRAQFMPAHQHDPEYAERTGLRLDGRDLIEEWKRAHPNGAYVWNTHQFNAAAGAPAL 306
Query: 203 LGLFAEGHLPYVLDRSEE---DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
LGLF H+ + DR + SL EMT+ AI+ L ++P+GF LM+EGGRIDHA HA +
Sbjct: 307 LGLFEPSHMQFEHDRDRHPNGEPSLAEMTRTAIQSLSRDPHGFVLMVEGGRIDHAHHAGN 366
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET D V + A + DTL+IV ADH
Sbjct: 367 AYRALDETIALSDAVRAAVQSAPK--DTLIIVTADH 400
>gb|AAF55830.1| (AE003733) CG10827 gene product [Drosophila melanogaster]
Length = 522
Score = 149 bits (372), Expect = 8e-35
Identities = 131/450 (29%), Positives = 198/450 (43%), Gaps = 72/450 (16%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYG----HLNMEDFPYTGIELTDSLSGEVTDSAAAGTAI 86
+NVI+ +GDGMG + + + G L+ E+FP+TG+ T S+ V DSA TA
Sbjct: 85 KNVILFLGDGMGVTTTSAARNLLGGEEKSLSFENFPFTGLSKTYSVDKIVPDSACTATAY 144
Query: 87 ATGVKTYNRMISTTN---------VTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPA 137
GVK I + + ++ ++ + A GK GLVTTTR+THA+P+
Sbjct: 145 LCGVKGQEGTIGVNGQVPRTDCKVMLDESTHVDSIAKWAMEAGKWAGLVTTTRVTHASPS 204
Query: 138 VFASHVPDRDMEEE-------------------IARQLILHNVTVLMGGGREKFSEEVLK 178
+H+ +RD E + +AR + + V+MGGGR+ F + L+
Sbjct: 205 GVYAHIAERDWENDAEVATDCGAGSGINDIAYQLARGEVGSKLKVIMGGGRKHFVDSSLE 264
Query: 179 ----------LAEDYGYS-----IVYTREDLEKV---KDGKVLGLFAEGHLPYVLDRSEE 220
L +++ + V T +L V K ++LGLF + HL Y+++ + E
Sbjct: 265 SWGKRTDGLNLIDEFKSASENNVYVTTAAELAAVDVTKTDRLLGLFNDDHLQYLMETTTE 324
Query: 221 DV--SLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETKEFDDVVGYVL 278
+L +MT+KAIE + +N G+FL IEGGRID A H N + ET EF +
Sbjct: 325 SSQPTLEQMTRKAIEYMSQNEQGYFLFIEGGRIDQAHHINQARMALNETIEFSKAIAAAQ 384
Query: 279 DYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMSKEIKSGGDIRD- 337
+ DTL++V ADH G +Y S + K +G D +
Sbjct: 385 ELT-SEDDTLLVVTADHSHVFTYAGYSYRGS-------------DVFGKTPVNGHDGKPY 430
Query: 338 -VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSHKHTGEPVPLL 396
V+ G + E E K+ T G+ E S GV H G+ VP+
Sbjct: 431 MVLSYANGPSYENFYDAETMERKDPTT-VVKGDHDDEFPS---GVPIDMDSHGGDDVPVY 486
Query: 397 AYGPGAENFVGFKHHVDTAKVIAKLMIFGD 426
A GP A F G ++A GD
Sbjct: 487 ALGPWAHLFTGVYEQSTIPHMMAYASCLGD 516
>dbj|BAA92181.1| (AB031539) endoderm-specific alkaline phosphatase [Ciona
intestinalis]
Length = 579
Score = 148 bits (371), Expect = 1e-34
Identities = 134/468 (28%), Positives = 213/468 (44%), Gaps = 87/468 (18%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVTDSA 80
+NVII IGDGMG + + +++ G L M+ PYTG+ T S++ +V+DSA
Sbjct: 55 KNVIIFIGDGMGVTTVTAGRILKGQNSGASGEETKLAMDKLPYTGVSRTYSVNRQVSDSA 114
Query: 81 AAGTAIATGVKTYNRMI-----STTNVTGKLVN----LTTLLEIAQMLGKATGLVTTTRI 131
+ TA TGVKT + ++ S + +N LT++L A+M G + G+VTTT I
Sbjct: 115 STATAFLTGVKTNDYVLGLNGNSVKGICAGSINESNLLTSVLLEAKMAGMSAGIVTTTTI 174
Query: 132 THATPAVFASHVPDR----DME----------EEIARQLI--LHNVTVLMGGGREKFSE- 174
HATPA ++ PDR D E ++IA+Q I TV++GGGR+ F
Sbjct: 175 NHATPAAAYANSPDRLWYSDAEMTAEAKENGCKDIAQQFIDKSDQFTVVLGGGRQYFKPN 234
Query: 175 ---------------------EVLKLAE-DYGYSIVYTREDLEKV---KDGKVLGLFAEG 209
EV K + D + V+ +E ++V K +LGLF
Sbjct: 235 TTFDVEYTDRANLRLDGQDLIEVWKAKQSDRNSAYVWNKEQFDQVDVAKTDSLLGLFEPS 294
Query: 210 HLPYVLDRSEE---DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
H+ Y R+++ + SL +MT KAI +L+KN GF L++EGG+IDH HA + +
Sbjct: 295 HMNYEAHRAQDGAGEPSLKDMTSKAIRMLKKNDQGFILLVEGGKIDHGHHAGKAYLALHD 354
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHE----TGGLGIGLN--YGHSVDIDSIRRIDA 320
DD + ++ +T++IV ADH GG N +G + ++++ + +D
Sbjct: 355 LVALDDAIEAAVEMT-SDDETMIIVTADHSHVFTIGGYSHRGNPIFGAAPNVNNPKLVDD 413
Query: 321 SIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLG 380
+ + + G + G+ + E + + K ++
Sbjct: 414 G-KPFTTLLYGNGPGHSTL---NGVGSCERENITLIATDDPNYK------------QQSA 457
Query: 381 VGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIFGDRS 428
V S H GE V ++A GP A F G A +I G +S
Sbjct: 458 VPLPSETHGGEDVVIMARGPMAHLFEGVHEQSYIAHIIRYATCIGKKS 505
>sp|P19147|PPB_SERMA ALKALINE PHOSPHATASE PRECURSOR (APASE) >gi|152841|gb|AAA26563.1|
(M33965) alkaline phosphatase (phoA) (EC 3.1.3.1)
[Serratia marcescens]
Length = 475
Score = 148 bits (371), Expect = 1e-34
Identities = 120/339 (35%), Positives = 165/339 (48%), Gaps = 69/339 (20%)
Query: 23 ANASPSGVRNVIILIGDGMGFSQLQLTKLVY----GHLN-MEDFPYTGIELTDSLSGE-- 75
A+ S +NVI+LIGDGMG S++ + G+ ++ P TG SL +
Sbjct: 61 ASLSDKTAKNVILLIGDGMGDSEITAARNYAEGAGGYFKGIDALPLTGQYTHYSLDRKTH 120
Query: 76 ----VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRI 131
VTDSAA+ TA ATGVKTYN + +V GK + TLLEIA+ GKATG V+T +
Sbjct: 121 KPDYVTDSAASATAWATGVKTYNGALGV-DVNGK--DQPTLLEIAKAAGKATGNVSTAEL 177
Query: 132 THATPAVFASHV-------PDRDMEE------------EIARQLILHNVTVLMGGGREKF 172
ATPA SHV P+ E+ I QL+ V +GGG + F
Sbjct: 178 QDATPAALVSHVISRKCYGPEETSEKCAANALENGGRGSITEQLLKTRADVTLGGGAKSF 237
Query: 173 SE----------EVLKLAEDYGYSIVYTREDLEKV----KDGKVLGLFAEGHLPYV---- 214
++ + A GY V ++L+ V + +LGLFA+G++P
Sbjct: 238 NQLAKSGEWQGKSLKDQAAAQGYQWVSNADELQAVTLANQQKPLLGLFADGNMPVRWLGP 297
Query: 215 -------LD-----------RSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACH 256
LD R+ +L MT+KAI +L+ NPNGFFL +EG ID H
Sbjct: 298 KASYHGNLDKPAVTCENNPARTAATPTLAAMTEKAIALLKDNPNGFFLQVEGASIDKQDH 357
Query: 257 ANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
A + + ET + D+ V L +AR G+TLVIV ADH
Sbjct: 358 AANPCGQIGETVDLDEAVQKALAFARADGNTLVIVTADH 396
>pir||T39459 repressible alkaline phosphatase precursor - fission yeast
(Schizosaccharomyces pombe) >gi|3184116|emb|CAA19331.1|
(AL023780) repressible alkaline phosphatase precursor
[Schizosaccharomyces pombe]
Length = 532
Score = 148 bits (369), Expect = 2e-34
Identities = 129/456 (28%), Positives = 219/456 (47%), Gaps = 58/456 (12%)
Query: 10 LLIFIIFSSM--FPFANASPSGVRN-----VIILIGDGMGFSQLQLTKLVYGHLNME--- 59
+L+ ++ S++ F F ++ S N VI+++ DGMG L +T+ LN +
Sbjct: 32 ILLILVLSTVIFFYFFSSHKSKGTNEKPKFVIMMVSDGMGPGSLSMTRSFVETLNDKEGY 91
Query: 60 ----DFPYTGIELTDSLSGEVTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEI 115
D G T S S +TDSAA TA + KTYN + V T+LE
Sbjct: 92 RLPLDEHLIGSSRTRSSSSLITDSAAGATAFSCANKTYNGAVG---VLDNEKPCGTILEA 148
Query: 116 AQMLGKATGLVTTTRITHATPAVFASHVPDRDMEEEIARQLIL-----HNVTVLMGGGRE 170
A+ G TG+V T+R+T ATPA F++H +R M++ IA + +V +L GGG
Sbjct: 149 AKEAGYLTGIVVTSRVTDATPASFSAHAANRFMQDLIAEYQVGMGPLGRSVDLLFGGGLC 208
Query: 171 KF------------SEEVLKLA-EDYGYSIVYTREDLEKVKDGKV--LGLFAEGHLPYVL 215
F + ++LK A + G+ I+ R D +++ + ++ LGLF++ HL Y +
Sbjct: 209 SFLPKSTYRSCRSDNLDLLKYARKKEGFQILLNRTDFDELSNAQLPLLGLFSDYHLSYDI 268
Query: 216 D-RSEEDVSLLEMTKKAIEIL-----EKNPNGFFLMIEGGRIDHACHANDVASIVAETKE 269
D + E L EM + A+++L E GFFL+IEG RID A H ND + V E E
Sbjct: 269 DYQPEVQPKLSEMVETALDVLLNATNEDTSKGFFLLIEGSRIDMASHNNDPIAHVYEVME 328
Query: 270 FDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGH------------SVDIDSIRR 317
++ + + G +L+ +DHETGGL +G S+ + SI
Sbjct: 329 YNRAFEIASAFVEKNGGSLIST-SDHETGGLTVGRQVSKKYPEYLWKPQVLSLALHSIEY 387
Query: 318 IDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISK 377
+ ++I ++ + V+ + +++ +I A++ N + L N++ +I+S
Sbjct: 388 LASAIVNHNQNTLLPYIEQFVLPAIGIPDPNPKQIHDIYVARH--NIFNLINVLSDIVSV 445
Query: 378 KLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVD 413
+ +G+ +H HT V + G E+ G +++
Sbjct: 446 EAQIGWTTHGHTAVDVNVYGVGEVTEHLRGNMENIE 481
>emb|CAA67052.1| (X98402) alkaline phosphatase [Drosophila melanogaster]
Length = 578
Score = 146 bits (364), Expect = 7e-34
Identities = 113/333 (33%), Positives = 163/333 (48%), Gaps = 69/333 (20%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGH-----------LNMEDFPYTGIELTDSLSGEVTDS 79
RN+II IGDGMG S + ++ G L +DFP TG+ T ++ +V DS
Sbjct: 85 RNIIIFIGDGMGISTISAGRIYKGQYLKHGYGEEETLVFDDFPNTGMAKTYNVDKQVPDS 144
Query: 80 AAAGTAIATGVKTYNRMI----STTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHAT 135
A TAI +G KT+ I + + G+ + +++E AQ GK TG+VTTTRITHAT
Sbjct: 145 AGTATAIFSGSKTHYGAIGMDATRSKKNGQQGRVQSVMEWAQKEGKRTGVVTTTRITHAT 204
Query: 136 PAVFASHVPDRDME-------------EEIARQLIL----HNVTVLMGGG---------- 168
PA +H+ DRD E +IARQL+ + V++GGG
Sbjct: 205 PAATYAHIYDRDWECDTEVPAESVGFHVDIARQLVENAPGNRFNVILGGGMSPMGILNAS 264
Query: 169 --------------------REKFSEEVLKLAED-YGYSIVYTREDLEKVKDGKV---LG 204
R +E + A D ++V+ R+DL V KV +G
Sbjct: 265 EVKTTIFEGPTETICTRGDNRNLPAEWLAHHANDTVPPALVHNRKDLLNVNVKKVDHLMG 324
Query: 205 LFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEKN--PNGFFLMIEGGRIDHACHANDVAS 262
LF H+ Y + R + SL EMT+ A+ ILE++ NGF L++EGGRIDH H N +
Sbjct: 325 LFRNNHITYSIAREAGEPSLQEMTETALGILERDDESNGFVLLVEGGRIDHGHHMNYARA 384
Query: 263 IVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ E EFD + ++ +TL++V ADH
Sbjct: 385 ALHELYEFDLAIQAAVNNT-DPDETLILVTADH 416
>sp|Q92058|PPBT_CHICK ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC ISOZYME PRECURSOR
(AP-TNAP) (LIVER/BONE/KIDNEY ISOZYME)
>gi|624283|gb|AAA92562.1| (U19108) tissue-nonspecific
alkaline phosphatase precursor [Gallus gallus]
Length = 519
Score = 144 bits (360), Expect = 2e-33
Identities = 112/331 (33%), Positives = 156/331 (46%), Gaps = 69/331 (20%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+N+I+ +GDGMG S + +++ G L M+ FPY + T + + +V DSA
Sbjct: 51 KNLILFLGDGMGVSTVTAARILKGQLQHRKGEESLLEMDKFPYVALAKTYNTNAQVPDSA 110
Query: 81 AAGTAIATGVKTYNRMISTT-NVTGKLVNLTTLLEIAQML------GKATGLVTTTRITH 133
TA GVK + + VT N T E+ +L GKA G+VTTTR+TH
Sbjct: 111 GTATAYLCGVKANEGTVGVSAGVTRDRCNTTKGQEVTSILRWAKDEGKAVGIVTTTRVTH 170
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGRE----KFS 173
ATP+ +H +RD ++IARQL+ + ++ V++GGGR+ K +
Sbjct: 171 ATPSAAYAHSANRDWYSDGEMPLDALEGGCKDIARQLVDNIPDIEVILGGGRKYMFPKNT 230
Query: 174 EEVLKLAEDY-------GYSIVYTREDLEKVKDGKV---------------------LGL 205
+V E+ G +V D + GKV LGL
Sbjct: 231 SDVEYPQEERHRGTRLDGKDLVQAWHDTKPA--GKVAKYVWHRRELLALNVSRVDFLLGL 288
Query: 206 FAEGHLPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIV 264
F G + Y LDR+ E D SL EM AI +L+KNP GFFL++EGGRIDH H +
Sbjct: 289 FEPGDMVYELDRNNETDPSLSEMVAVAIRMLQKNPRGFFLLVEGGRIDHGHHEGKAKQAL 348
Query: 265 AETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
E E D VG R DTL +V ADH
Sbjct: 349 HEAVELDRAVGLAGRLTSPR-DTLSVVTADH 378
>pdb|1ANI|A Chain A, Alkaline Phosphatase (D153h, K328h) >gi|1310911|pdb|1ANI|B
Chain B, Alkaline Phosphatase (D153h, K328h)
Length = 446
Score = 144 bits (359), Expect = 3e-33
Identities = 108/336 (32%), Positives = 159/336 (47%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 35 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 94
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + HA
Sbjct: 95 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQHA 151
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 152 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 211
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 212 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 271
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG IDH HA +
Sbjct: 272 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDHQDHAAN 331
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 332 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 367
>sp|P21948|PPB_ESCFE ALKALINE PHOSPHATASE PRECURSOR (APASE) >gi|2147105|pir||I57445
alkaline phosphatase (EC 3.1.3.1) (phoA) - Escherichia
fergusonii >gi|147245|gb|AAA24371.1| (M33966) alkaline
phosphatase (phoA) (EC 3.1.3.1) [Escherichia fergusonii]
Length = 472
Score = 143 bits (356), Expect = 6e-33
Identities = 137/457 (29%), Positives = 191/457 (40%), Gaps = 120/457 (26%)
Query: 23 ANASPSGVRNVIILIGDGMGFSQLQLTKLVY----GHLN-MEDFPYTGIEL---TDSLSG 74
A+ + +N+I+LIGDGMG S++ + G+ ++ P TG D +G
Sbjct: 58 ASLNDKPAKNIILLIGDGMGDSEITAARNYAEGAGGYFKGIDALPLTGQYTHYALDKKTG 117
Query: 75 E---VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRI 131
+ VTDSAA+ TA TGVKTYN + TT+LE+A+ G ATG V+T +
Sbjct: 118 KPDYVTDSAASATAWTTGVKTYNGALGVDIHENPH---TTILEMAKAAGLATGNVSTAEL 174
Query: 132 THATPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKF 172
ATPA SHV R + I QL+ V +GGG + F
Sbjct: 175 QDATPAALVSHVTSRKCYGPSVTSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTF 234
Query: 173 SEEVL----------KLAEDYGYSIVYTREDLEKV----KDGKVLGLFAEGHLPYV---- 214
+E + A GY IV L V +D +LGLFAEG++P
Sbjct: 235 AETATAGEWQGKTLREQALARGYQIVSDAASLAAVTQAGQDKPLLGLFAEGNMPVRWHGP 294
Query: 215 -------LD-----------RSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACH 256
LD R+E +L +MT KAIE+L KN GFFL +EG ID H
Sbjct: 295 KASYHGNLDKPAVTCTPNPQRNETVPTLAQMTDKAIELLSKNERGFFLQVEGASIDKQDH 354
Query: 257 ANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIR 316
A + + ET + D+ V L++A++ G+TLVIV ADH HS I
Sbjct: 355 AANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH-----------AHSSQI---- 399
Query: 317 RIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIIS 376
++ + K+ G + L D V I + +
Sbjct: 400 --------VAPDTKAPGLTQ-------ALNTKDGAVMAISYGNSEED------------- 431
Query: 377 KKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVD 413
S +HTG + + AYGP A N VG D
Sbjct: 432 --------SQEHTGSQLRIAAYGPNAANVVGLTDQTD 460
>gb|AAF57106.1| (AE003776) Aph-4 gene product [Drosophila melanogaster]
Length = 596
Score = 142 bits (355), Expect = 7e-33
Identities = 112/333 (33%), Positives = 161/333 (47%), Gaps = 69/333 (20%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGH-----------LNMEDFPYTGIELTDSLSGEVTDS 79
RN+II IGDGMG S + ++ G L +DFP TG+ T ++ +V DS
Sbjct: 85 RNIIIFIGDGMGISTISAGRIYKGQYLKHGYGEEETLVFDDFPNTGMAKTYNVDKQVPDS 144
Query: 80 AAAGTAIATGVKTYNRMI----STTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHAT 135
A TAI +G KT+ I + + G+ + +++E AQ GK TG+VTTTRITHAT
Sbjct: 145 AGTATAIFSGSKTHYGAIGMDATRSKKNGQQGRVQSVMEWAQKEGKRTGVVTTTRITHAT 204
Query: 136 PAVFASHVPDRDME-------------EEIARQLIL----HNVTVLMGGG---------- 168
PA +H+ DRD E +IARQL+ + V++GGG
Sbjct: 205 PAATYAHIYDRDWECDTEVPAESVGFHVDIARQLVENAPGNRFNVILGGGMSPMGILNAS 264
Query: 169 --------------------REKFSEEVLKLAED-YGYSIVYTREDLEKVKDGKV---LG 204
R +E + A D ++V+ R+DL V KV +G
Sbjct: 265 EVKTTIFEGPTETICTRGDNRNLPAEWLAHHANDTVPPALVHNRKDLLNVNVKKVDHLMG 324
Query: 205 LFAEGHLPYVLDRSEEDVSLLEMTKKAIEILEK--NPNGFFLMIEGGRIDHACHANDVAS 262
LF H+ Y + R + SL EMT+ A+ ILE+ NGF L++EGGRID H N +
Sbjct: 325 LFRNNHITYSIAREAGEPSLQEMTETALGILERGDESNGFVLLVEGGRIDQGHHMNYARA 384
Query: 263 IVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ E EFD + ++ +TL++V ADH
Sbjct: 385 ALHELYEFDLAIQAAVNNT-DPDETLILVTADH 416
>gb|AAF58927.1| (AE003833) CG1809 gene product [Drosophila melanogaster]
Length = 515
Score = 141 bits (353), Expect = 1e-32
Identities = 111/344 (32%), Positives = 157/344 (45%), Gaps = 64/344 (18%)
Query: 15 IFSSMFPFANASPSGVRNVIILIGDGMGFSQLQLTKLVYG----HLNMEDFPYTGIELTD 70
+ S + +A+ + +NVI+ +GDGM + T+ + G + E FPYTG+ T
Sbjct: 57 LLSKLAEQESATTNKAKNVILFLGDGMSVHTVTATRNLLGDSAEQVYFEGFPYTGLSKTY 116
Query: 71 SLSGEVTDSAAAGTAIATGVKT------YNRMISTTNVTGKLVNLTTLLEIA---QMLGK 121
++ +V DSA TA GVK N +S + G +L IA Q GK
Sbjct: 117 CVNRQVADSACTATAYLGGVKANYGTIGVNANVSRYSCDGAANEEDRVLSIAHWAQAAGK 176
Query: 122 ATGLVTTTRITHATPAVFASHVPDRDMEEE---------------IARQLILHNV----T 162
GLVTT R+THA+PA +H+ DR+ E + IARQL+ V
Sbjct: 177 DAGLVTTARVTHASPAGVYAHIADRNWENDWEVANRECDPEQTIDIARQLVEQPVGQQLK 236
Query: 163 VLMGGGREKFSEEVLKLAEDY-----------------------GYSIVYTREDLEKV-- 197
V++GGGR+ F + + E Y V++R+ L V
Sbjct: 237 VILGGGRKNFIDATVNDEEGYPGKRTDGRHLIRSWLDQKKEANVSAKYVWSRKGLSLVDL 296
Query: 198 -KDGKVLGLFAEGHLPYVLDRSEE-----DVSLLEMTKKAIEILEKNPNGFFLMIEGGRI 251
+LGLFA HLPY DR + D SL E+T+ AI +L +N GFFL +EG RI
Sbjct: 297 ENTDYLLGLFANDHLPYNGDRDRKRSQLADPSLSELTEAAITVLSRNDKGFFLFVEGARI 356
Query: 252 DHACHANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
D A H + +T EF V + DTL++V ADH
Sbjct: 357 DMAHHDTFAKRSLEDTAEFARAVQKARELT-SEDDTLIVVTADH 399
>gb|AAD37431.1|AF149417_1 (AF149417) alkaline phosphatase [Canis familiaris]
Length = 383
Score = 141 bits (352), Expect = 2e-32
Identities = 110/340 (32%), Positives = 156/340 (45%), Gaps = 73/340 (21%)
Query: 24 NASPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLS 73
N + + +NVI+ +GDGMG S + T+++ G L+ M+ FPY + T + +
Sbjct: 27 NLNTNVAKNVIMFLGDGMGVSTVTATRILKGQLHHNPGEETRLEMDKFPYVALSKTYNTN 86
Query: 74 GEVTDSAAAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLV 126
+V DSA TA GVK + + T + +T++L A+ GK+ G+V
Sbjct: 87 AQVPDSAGTATAYLCGVKANEGTVGVSAATQRTQCNTTQGNEVTSILRWAKDAGKSVGIV 146
Query: 127 TTTRITHATPAVFASHVPDRDME--------------EEIARQLILHNV---TVLMGGGR 169
TTTR+ HATP+ +H DRD ++IA QL +HNV V+MGGGR
Sbjct: 147 TTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQL-MHNVKDIEVIMGGGR 205
Query: 170 EKFSEEVLKLAEDYGYSI------------------------------VYTREDLEKVKD 199
+ K D Y + V+ R +L +
Sbjct: 206 KYMFP---KNRTDVEYEMDEKSRGTRLDGLNLIDIWKNFKPRHKHSHYVWNRTELLALDP 262
Query: 200 GKV---LGLFAEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHAC 255
V LGLF G + Y L+R+ D SL EM + AI+IL KNP GFFL++EGGRIDH
Sbjct: 263 YTVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVEIAIKILSKNPRGFFLLVEGGRIDHGH 322
Query: 256 HANDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
H + E E D +G DTL +V ADH
Sbjct: 323 HEGKAKQALHEAVEMDRAIGKA-GVMTSLEDTLTVVTADH 361
>sp|P09487|PPBT_BOVIN ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC ISOZYME PRECURSOR
(AP-TNAP) (LIVER/BONE/KIDNEY ISOZYME)
>gi|89381|pir||A29600 alkaline phosphatase (EC 3.1.3.1)
precursor, hepatic - bovine >gi|162676|gb|AAA30380.1|
(M18443) alkaline phosphatase precursor (EC 3.1.3.2)
[Bos taurus]
Length = 524
Score = 141 bits (351), Expect = 2e-32
Identities = 106/332 (31%), Positives = 151/332 (44%), Gaps = 71/332 (21%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGMG S + +++ G L+ M+ FPY + T + + +V DSA
Sbjct: 52 KNVIMFLGDGMGVSTVTAARILKGQLHHSPGEETKLEMDKFPYVALSKTYNTNAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
TA GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATAYLCGVKANEGTVGVSAATQRSQCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGREKFSEEVL 177
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR+
Sbjct: 172 ATPSASYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMYNIKDIEVIMGGGRKYMFP--- 228
Query: 178 KLAEDYGYSI------------------------------VYTREDLEKVKDGKV---LG 204
K D Y + V+ R DL + V LG
Sbjct: 229 KNRTDVEYELDEKARGTRLDGLNLIDIWKSFKPKHKHSHYVWNRTDLLALDPHSVDYLLG 288
Query: 205 LFAEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASI 263
LF G + Y L+R+ D SL EM + AI IL KNP GFFL++EGGRIDH H
Sbjct: 289 LFEPGDMQYELNRNNATDPSLSEMVEMAIRILNKNPKGFFLLVEGGRIDHGHHEGKAKQA 348
Query: 264 VAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ E E D +G DTL +V ADH
Sbjct: 349 LHEAVEMDQAIGQA-GAMTSVEDTLTVVTADH 379
>pdb|1ANJ|A Chain A, Alkaline Phosphatase (K328h) >gi|1310909|pdb|1ANJ|B Chain
B, Alkaline Phosphatase (K328h)
Length = 446
Score = 140 bits (350), Expect = 3e-32
Identities = 107/336 (31%), Positives = 158/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 35 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 94
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 95 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 151
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 152 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 211
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 212 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 271
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG IDH HA +
Sbjct: 272 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDHQDHAAN 331
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 332 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 367
>pdb|2ANH|A Chain A, Alkaline Phosphatase (D153h) >gi|1310739|pdb|2ANH|B Chain
B, Alkaline Phosphatase (D153h)
Length = 446
Score = 140 bits (350), Expect = 3e-32
Identities = 107/336 (31%), Positives = 158/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 35 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 94
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + HA
Sbjct: 95 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQHA 151
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 152 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 211
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 212 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 271
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 272 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 331
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 332 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 367
>gi|7106246 tissue-nonspecific ALP alkaline phosphatase [Rattus norvegicus]
>gi|91938|pir||A28114 alkaline phosphatase (EC 3.1.3.1)
precursor - rat >gi|206123|gb|AAA41845.1| (J03572)
alkaline phosphatase [Rattus norvegicus]
Length = 524
Score = 140 bits (350), Expect = 3e-32
Identities = 102/332 (30%), Positives = 155/332 (45%), Gaps = 71/332 (21%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+N+I+ +GDGMG S + +++ G L+ M+ FP+ + T + + +V DSA
Sbjct: 52 KNIIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
TA GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGREKFSEEVL 177
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR+
Sbjct: 172 ATPSAAYAHSADRDWYSDNEMRPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYP--- 228
Query: 178 KLAEDYGYSI------------------------------VYTREDLEKVKDGKV---LG 204
K D Y + V+ R +L + +V LG
Sbjct: 229 KNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLG 288
Query: 205 LFAEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASI 263
LF G + Y L+R+ D SL EM + A+ IL KNP GFFL++EGGRIDH H
Sbjct: 289 LFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNPKGFFLLVEGGRIDHGHHEGKAKQA 348
Query: 264 VAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ E E D+ +G ++ DTL +V ADH
Sbjct: 349 LHEAVEMDEAIGKAGTMTSQK-DTLTVVTADH 379
>sp|Q29486|PPBT_FELCA ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC ISOZYME PRECURSOR
(AP-TNAP) (LIVER/BONE/KIDNEY ISOZYME)
>gi|2136460|pir||S66467 alkaline phosphatase (EC
3.1.3.1) - cat >gi|947119|gb|AAA82993.1| (U31569)
alkaline phosphatase [Felis catus]
Length = 524
Score = 140 bits (350), Expect = 3e-32
Identities = 111/331 (33%), Positives = 155/331 (46%), Gaps = 67/331 (20%)
Query: 30 VRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDS 79
V+NVI+ +GDGMG S + +++ G L+ M+ FPY + T + + +V DS
Sbjct: 51 VKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPYVALSKTYNTNAQVPDS 110
Query: 80 AAAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRIT 132
A TA GVK + + T + +T++L A+ GK+ G+VTTTR+
Sbjct: 111 AGTATAYLCGVKANEGTVGVSAATQRTQCNTTQGNEVTSILRWAKDSGKSVGIVTTTRVN 170
Query: 133 HATPAVFASHVPDRDME--------------EEIARQLILHNV---TVLMGGGR------ 169
HATP+ +H DRD ++IA QL +HNV V+MGGGR
Sbjct: 171 HATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQL-MHNVRDIEVIMGGGRKYMFPK 229
Query: 170 -----EKFSEEVLKLAEDYGYSI----------------VYTREDLEKVKDGKV---LGL 205
E +E + G ++ V+ R +L + V LGL
Sbjct: 230 NRTDVEYEMDEKARGTRLDGLNLVDIWKSFKPRHKHSHYVWNRTELLTLDPYGVDYLLGL 289
Query: 206 FAEGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIV 264
F G + Y L+R S D SL EM + AI+IL KNP GFFL++EGGRIDH H +
Sbjct: 290 FEPGDMQYELNRNSTTDPSLSEMVEIAIKILSKNPKGFFLLVEGGRIDHGHHEGKAKQAL 349
Query: 265 AETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
E E D +G DTL IV ADH
Sbjct: 350 HEAVEMDQAIGRA-GAMTSVEDTLTIVTADH 379
>sp|P08289|PPBT_RAT ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC ISOZYME PRECURSOR
(AP-TNAP) (LIVER/BONE/KIDNEY ISOZYME) (TNSALP)
>gi|3901092|emb|CAA34160.1| (X16028) alkaline
phosphatase [Rattus norvegicus]
Length = 524
Score = 140 bits (350), Expect = 3e-32
Identities = 102/332 (30%), Positives = 155/332 (45%), Gaps = 71/332 (21%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+N+I+ +GDGMG S + +++ G L+ M+ FP+ + T + + +V DSA
Sbjct: 52 KNIIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
TA GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGREKFSEEVL 177
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR+
Sbjct: 172 ATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYP--- 228
Query: 178 KLAEDYGYSI------------------------------VYTREDLEKVKDGKV---LG 204
K D Y + V+ R +L + +V LG
Sbjct: 229 KNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLG 288
Query: 205 LFAEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASI 263
LF G + Y L+R+ D SL EM + A+ IL KNP GFFL++EGGRIDH H
Sbjct: 289 LFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNPKGFFLLVEGGRIDHGHHEGKAKQA 348
Query: 264 VAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ E E D+ +G ++ DTL +V ADH
Sbjct: 349 LHEAVEMDEAIGKAGTMTSQK-DTLTVVTADH 379
>gb|AAF50764.1| (AE003565) CG5150 gene product [Drosophila melanogaster]
Length = 517
Score = 140 bits (350), Expect = 3e-32
Identities = 127/451 (28%), Positives = 195/451 (43%), Gaps = 70/451 (15%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYG----HLNMEDFPYTGIELTDSLSGEVTDSAAAGTAI 86
+N+I+ +GDGMG + L + G L+ E+FP+TG+ T S+ V DSA T+
Sbjct: 76 KNIILFLGDGMGLATLAAARSYIGGEELKLSFEEFPFTGLSKTYSVDKIVPDSACTSTSY 135
Query: 87 ATGVKTYNRMISTTN---------VTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPA 137
GVK I + + ++ +L + A GKA GLVTTTR+THA+P+
Sbjct: 136 LCGVKANYGTIGVNAHVKRGDCAAMANETNHVFSLGKWAMDAGKAAGLVTTTRVTHASPS 195
Query: 138 VFASHVPDRDME----------------EEIARQLILHNV----TVLMGGGREKFSE--- 174
+HV DR+ E ++IA QLI V V++GGG+ F
Sbjct: 196 GVYAHVADREWENNAVLEEACGELSDGLQDIAVQLIHGEVGSKLKVMLGGGKRSFYSPEH 255
Query: 175 -------EVLKLAEDY-----GYSIVYTREDLEKV---KDGKVLGLFAEGHLPYVL---- 215
+ L E++ G + V T++ L V + G++LGLF++ H+ Y L
Sbjct: 256 YDKGRRTDGRNLVEEFEALDAGNTFVKTQKKLLNVNATETGRLLGLFSKSHMHYHLEQLA 315
Query: 216 DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETKEFDDVVG 275
D + +L EMT+KAIE+LE G+FL +EGG+ID + H + ET E V
Sbjct: 316 DPDNNEPTLEEMTQKAIEVLETEEQGYFLFVEGGKIDISHHETMARIALDETAELSKAVK 375
Query: 276 YVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMSKEIKSGGDI 335
+ +TL++V +DH H+ + + + I +K G
Sbjct: 376 KAREMTNPE-ETLIVVTSDH-----------SHTFSVSGYQPRGSDIFGAAKANGKDGKP 423
Query: 336 RDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSHKHTGEPVPL 395
+ G D E E + T+ +G+ + V S H GE V +
Sbjct: 424 YLALSYANGKSFQDYYNTETHERVDPTSLPTIGDFDQLFPAM---VPLESETHGGEDVGV 480
Query: 396 LAYGPGAENFVGFKHHVDTAKVIAKLMIFGD 426
A GP A F G ++A GD
Sbjct: 481 FASGPWAHLFTGVYEQNTIPHMMAFAACVGD 511
>gi|4502063 alkaline phosphatase, liver/bone/kidney; tissue-nonspecific ALP
[Homo sapiens] >gi|178462|gb|AAB59378.1| (M24439)
alkaline phosphatase [Homo sapiens]
Length = 524
Score = 139 bits (347), Expect = 6e-32
Identities = 105/329 (31%), Positives = 153/329 (45%), Gaps = 65/329 (19%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGMG S + +++ G L+ M+ FP+ + T + + +V DSA
Sbjct: 52 KNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
TA GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGR-------- 169
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR
Sbjct: 172 ATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNK 231
Query: 170 ---EKFSEEVLKLAEDYGYSIV----------------YTREDLEKVKDGKV---LGLFA 207
E S+E + G +V + R +L + V LGLF
Sbjct: 232 TDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFE 291
Query: 208 EGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y L+R+ D SL EM AI+IL KNP GFFL++EGGRIDH H + E
Sbjct: 292 PGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHE 351
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADH 295
E D +G DTL +V ADH
Sbjct: 352 AVEMDRAIGQAGSLTSSE-DTLTVVTADH 379
>emb|CAA07382.1| (AJ006985) StrK [Streptomyces glaucescens]
Length = 460
Score = 139 bits (347), Expect = 6e-32
Identities = 118/338 (34%), Positives = 152/338 (44%), Gaps = 69/338 (20%)
Query: 27 PSGVRNVIILIGDGMGFSQL----QLTKLVYGHLNMEDFPYTGIELTDSLSGE-----VT 77
P+ RNVI+L+GDGMG S++ T G L M+ P TG LT ++ VT
Sbjct: 48 PARPRNVILLVGDGMGDSEITAARNYTVGAGGRLAMDRLPMTGSSLTYAVDERGRPDYVT 107
Query: 78 DSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPA 137
DSAA TA ATG KT N ++ T T + L TLLE+A+ G ATG VTT R+T ATPA
Sbjct: 108 DSAAGATAWATGHKTVNGRVAKTPGTDR--PLPTLLELARRQGYATGSVTTARLTDATPA 165
Query: 138 VFASHVPDR-------------DMEE-----EIARQLILHNVTVLMGGGREKFSEE---- 175
+HV DR D +E IA Q + VL+GGG + F +
Sbjct: 166 ALTAHVTDRSCAGPADMAACPADAKERGGAGSIAEQTVALRPDVLLGGGADVFGQRITAG 225
Query: 176 ------VLKLAEDYGYSIVYTREDLEKVK-DGKVLGLFAEGHLPY--------------- 213
VL+ A GY +V R L + D VLGLFA+G LP
Sbjct: 226 PHRGRTVLERARAEGYQVVRDRTGLRAARPDRPVLGLFADGPLPVEWTGVPARPGGTAPQ 285
Query: 214 -----VLDRSEEDVSLLEMTKKAIEILEKN-------PNGFFLMIEGGRIDHACHANDVA 261
SL E T+ A+E+L GFFL +EG ID H D
Sbjct: 286 RCGTGAPAHPAGTPSLEESTRAALELLTARARRAGAAAGGFFLQVEGAAIDDRAHDADPC 345
Query: 262 SIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGG 299
+ ET FD VG + R ++V+ DH +GG
Sbjct: 346 GQIGETVAFDRAVGQ--RWPMRTHPGTLVVVTDHGSGG 381
>gi|11424592 alkaline phosphatase, liver/bone/kidney [Homo sapiens]
Length = 482
Score = 139 bits (347), Expect = 6e-32
Identities = 105/329 (31%), Positives = 153/329 (45%), Gaps = 65/329 (19%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGMG S + +++ G L+ M+ FP+ + T + + +V DSA
Sbjct: 52 KNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
TA GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGR-------- 169
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR
Sbjct: 172 ATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNK 231
Query: 170 ---EKFSEEVLKLAEDYGYSIV----------------YTREDLEKVKDGKV---LGLFA 207
E S+E + G +V + R +L + V LGLF
Sbjct: 232 TDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFE 291
Query: 208 EGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y L+R+ D SL EM AI+IL KNP GFFL++EGGRIDH H + E
Sbjct: 292 PGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHE 351
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADH 295
E D +G DTL +V ADH
Sbjct: 352 AVEMDRAIGQAGSLTSSE-DTLTVVTADH 379
>pir||A83235 alkaline phosphatase PA3296 [imported] - Pseudomonas aeruginosa
(strain PAO1) >gi|9949423|gb|AAG06684.1|AE004751_10
(AE004751) alkaline phosphatase [Pseudomonas aeruginosa]
Length = 476
Score = 139 bits (346), Expect = 8e-32
Identities = 111/336 (33%), Positives = 158/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVY----GHLN-MEDFPYTG------IELTDSLSG 74
S +NVI+LIGDGMG S++ + + G+ ++ P TG + L
Sbjct: 64 SKKKAKNVILLIGDGMGDSEITVARNYARGAGGYFKGIDALPLTGQYTHYSLHKDSGLPD 123
Query: 75 EVTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVK+YN I NL LE+A++ GKATG V+T + A
Sbjct: 124 YVTDSAASATAWSTGVKSYNGAIGVDIHEQPHRNL---LELAKLNGKATGNVSTAELQDA 180
Query: 135 TPAVFASHV---------------PDRDMEE----EIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV P +E I Q + V++GGG F+E
Sbjct: 181 TPAALLAHVTARKCYGPEATSKQCPSNALENGGAGSITEQWLKTRPDVVLGGGAATFAET 240
Query: 176 ----------VLKLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ AE GY IV ++L+ V+ ++GLFA G++P
Sbjct: 241 AKAGRYAGKTLRAQAEARGYRIVENLDELKAVRRANQKQPLIGLFAPGNMPVRWLGPTAT 300
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R+ + +L +MT KAIE+L+ NPNGFFL +EG ID HA +
Sbjct: 301 YHGNLNQPAVSCEANPKRTADIPTLAQMTSKAIELLKDNPNGFFLQVEGASIDKQDHAAN 360
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L +A+ G+TLVIV ADH
Sbjct: 361 PCGQIGETVDLDEAVQKALAFAKADGETLVIVTADH 396
>sp|P05186|PPBT_HUMAN ALKALINE PHOSPHATASE, TISSUE-NONSPECIFIC ISOZYME PRECURSOR
(AP-TNAP) (LIVER/BONE/KIDNEY ISOZYME) (TNSALP)
>gi|1070485|pir||PAHUH alkaline phosphatase (EC 3.1.3.1)
precursor, hepatic - human >gi|28738|emb|CAA32376.1|
(X14174) alkaline phosphatase precursor (AA -17 to 507)
[Homo sapiens]
Length = 524
Score = 139 bits (346), Expect = 8e-32
Identities = 105/329 (31%), Positives = 152/329 (45%), Gaps = 65/329 (19%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGMG S + +++ G L+ M+ FP+ + T + +V DSA
Sbjct: 52 KNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTKAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
TA GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGR-------- 169
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR
Sbjct: 172 ATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNK 231
Query: 170 ---EKFSEEVLKLAEDYGYSIV----------------YTREDLEKVKDGKV---LGLFA 207
E S+E + G +V + R +L + V LGLF
Sbjct: 232 TDVEYESDEKARGTRLDGLDLVDTWKSFKPRHKHSHFIWNRTELLTLDPHNVDYLLGLFE 291
Query: 208 EGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y L+R+ D SL EM AI+IL KNP GFFL++EGGRIDH H + E
Sbjct: 292 PGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHE 351
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADH 295
E D +G DTL +V ADH
Sbjct: 352 AVEMDRAIGQAGSLTSSE-DTLTVVTADH 379
>dbj|BAA32129.1| (AB011406) alkalin phosphatase [Homo sapiens]
Length = 524
Score = 139 bits (346), Expect = 8e-32
Identities = 104/329 (31%), Positives = 153/329 (45%), Gaps = 65/329 (19%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGMG S + +++ G L+ M+ FP+ + T + + +V DSA
Sbjct: 52 KNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
TA GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGR-------- 169
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR
Sbjct: 172 ATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNK 231
Query: 170 ---EKFSEEVLKLAEDYGYSIV----------------YTREDLEKVKDGKV---LGLFA 207
E S+E + G +V + R +L + V LG F
Sbjct: 232 TDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGFFE 291
Query: 208 EGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y L+R+ D SL EM AI+IL KNP GFFL++EGGRIDH H + E
Sbjct: 292 PGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHE 351
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADH 295
E D +G+ DTL +V ADH
Sbjct: 352 AVEMDRAIGHAGSLTSSE-DTLTVVTADH 379
>gb|AAF64516.1| (AF250846) tissue non-specific alkaline phosphatase [Canis
familiaris]
Length = 502
Score = 138 bits (345), Expect = 1e-31
Identities = 110/337 (32%), Positives = 157/337 (45%), Gaps = 67/337 (19%)
Query: 24 NASPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLS 73
N + + +NVI+ +GDGMG S + T+++ G L+ M+ FPY + T + +
Sbjct: 23 NLNTNVAKNVIMFLGDGMGVSTVTATRILKGQLHHNPGEETRLEMDKFPYVALSKTYNTN 82
Query: 74 GEVTDSAAAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLV 126
+V DSA TA GVK + + T + +T++L A+ GK+ G+V
Sbjct: 83 AQVPDSAGTATAYLCGVKANEGTVGVSAATQRTHCNTTQGNEVTSILRWAKDAGKSVGIV 142
Query: 127 TTTRITHATPAVFASHVPDRDME--------------EEIARQLILHNV---TVLMGGGR 169
TTTR+ HATP+ +H DRD ++IA QL +HNV V+MGGGR
Sbjct: 143 TTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQL-MHNVKDIEVIMGGGR 201
Query: 170 -----------EKFSEEVLKLAEDYGYSI----------------VYTREDLEKVKDGKV 202
E +E A G ++ V+ R +L + V
Sbjct: 202 KYMFPKNRTDVEYEMDEKSTGARLDGLNLIDIWKNFKPRHKHSHYVWNRTELLALDPYTV 261
Query: 203 ---LGLFAEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAN 258
LGLF G + Y L+R+ D SL EM + AI+IL K P GFFL++EGGRIDH H
Sbjct: 262 DYLLGLFDPGDMQYELNRNNVTDPSLSEMVEIAIKILSKKPRGFFLLVEGGRIDHGHHEG 321
Query: 259 DVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ E E D +G DTL +V ADH
Sbjct: 322 KAKQALHEAVEMDRAIGKA-GVMTSLEDTLTVVTADH 357
>gb|AAF51724.1| (AE003594) CG5656 gene product [Drosophila melanogaster]
Length = 535
Score = 138 bits (344), Expect = 1e-31
Identities = 112/362 (30%), Positives = 162/362 (43%), Gaps = 73/362 (20%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVI +GDGM + ++ G L E F Y G+ T ++ +V DSA
Sbjct: 65 KNVIFFLGDGMSVPTVTAGRIFDGQLRGVVGERNRLEFEKFNYVGLSKTYCVNKQVADSA 124
Query: 81 AAGTAIATGVKTYNRMISTT------NVTGKLVNLTTLLEIAQML---GKATGLVTTTRI 131
+A +G+K I T + G + L IA K+ GLVTTTR+
Sbjct: 125 CTASAYLSGIKANYLTIGVTADVELNDCRGSRLPQNRLSSIAAWALKGSKSAGLVTTTRV 184
Query: 132 THATPAVFASHVPDRDMEEE---------------IARQLILHNV----TVLMGGGREKF 172
THA+PA +H +RD E + IA+QLI +V V+MGGG KF
Sbjct: 185 THASPAGVYAHTSNRDFESDYDVTKLGQNPGNCPDIAQQLIDGDVGKRLRVVMGGGTIKF 244
Query: 173 SEEVLK--------------LAEDYGYS------IVYTREDL---EKVKDGKVLGLFAEG 209
L E++ + +V++R DL + +LGLF
Sbjct: 245 LPNTTNDVFGNKGQRLDNRNLIEEWQQTKPGNARVVFSRTDLLNNDAQNTDFLLGLFNAS 304
Query: 210 HLPYVLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETKE 269
HL Y +D S + +L EMT AI +L ++PNG+FL +EGGRIDHA H + ET +
Sbjct: 305 HLAYHMDDSIDTPTLPEMTAAAINVLSRDPNGYFLFVEGGRIDHAHHETKAKKALDETVQ 364
Query: 270 FDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMSKEI 329
F D V DTL++V ADH GH+V I ++ SI ++ E+
Sbjct: 365 FSDAVRKARQLT-NPWDTLIVVSADH-----------GHTVTISGYPDVNNSIVGLNSEL 412
Query: 330 KS 331
+
Sbjct: 413 SN 414
>gb|AAC33182.1| (U35316) alkaline phosphatase with N-terminal PelB-leader and
C-terminal hexa-histidine tag; The coding region for the
PelB-leader is followed by a polylinker and the E.coli
JM81 alkaline phosphatase gene (ecphoA1-gene)
respectively. Additionally the c>
Length = 491
Score = 138 bits (344), Expect = 1e-31
Identities = 120/429 (27%), Positives = 189/429 (43%), Gaps = 79/429 (18%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 73 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 132
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 133 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 189
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 190 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 249
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 250 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 309
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 310 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 369
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRID 319
+ ET + D+ V L++A++ G+TLVIV ADH + + ++ D
Sbjct: 370 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKD 429
Query: 320 ASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
++ MS G+ + + HTG +L + ++ N L + + K
Sbjct: 430 GAVMVMSY-----GNSEEDSQEHTGSQL-----RIAAYGPHAANVVGLTDQTDLFYTMKA 479
Query: 380 GVGFVSHKH 388
+G ++H H
Sbjct: 480 ALGDIAHHH 488
>pir||S00289 alkaline phosphatase (EC 3.1.3.1), hepatic precursor - rat
>gi|55671|emb|CAA68703.1| (Y00714) precursor polypeptide
(AA -17 to 507) [Rattus norvegicus]
Length = 524
Score = 138 bits (343), Expect = 2e-31
Identities = 101/330 (30%), Positives = 154/330 (46%), Gaps = 67/330 (20%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+N+I+ +GDGMG S + +++ G L+ M+ FP+ + T + + +V DSA
Sbjct: 52 KNIIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
T GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATCYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGREKFSEEVL 177
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR K+
Sbjct: 172 ATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGR-KYMYPKN 230
Query: 178 KLAEDYGYS----------------------------IVYTREDLEKVKDGKV---LGLF 206
+ E+Y V+ R +L + +V LGLF
Sbjct: 231 RTDEEYELDEKARGTRLDGLDLITIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLF 290
Query: 207 AEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVA 265
G + Y L+R+ D SL EM + A+ IL KNP GFFL+ EGGRIDH H +
Sbjct: 291 EPGDMQYELNRNNLTDPSLSEMVEVALRILTKNPKGFFLLEEGGRIDHGHHEGKAKQALH 350
Query: 266 ETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
E E D+ +G ++ DTL +V ADH
Sbjct: 351 EAVEMDEAIGKAGTMTSQK-DTLTLVTADH 379
>pdb|1ALK|A Chain A, Alkaline Phosphatase (E.C.3.1.3.1) >gi|493812|pdb|1ALK|B
Chain B, Alkaline Phosphatase (E.C.3.1.3.1)
Length = 449
Score = 138 bits (343), Expect = 2e-31
Identities = 107/336 (31%), Positives = 157/336 (45%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSQKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ AE GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREEAEARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>pdb|1AJB|A Chain A, Mol_id: 1; Molecule: Alkaline Phosphatase; Chain: A, B;
Ec: 3.1.3.1; Engineered: Yes; Mutation: D153g;
Heterogen: Zinc Ion; Heterogen: Magnesium Ion;
Other_details: Mutant With 4-Fold Increased Activity And
Weaker Mg Binding >gi|1311185|pdb|1AJB|B Chain B,
Mol_id: 1; Molecule: Alkaline Phosphatase; Chain: A, B;
Ec: 3.1.3.1; Engineered: Yes; Mutation: D153g;
Heterogen: Zinc Ion; Heterogen: Magnesium Ion;
Other_details: Mutant With 4-Fold Increased Activity And
Weaker Mg Binding >gi|1311183|pdb|1AJC|B Chain B,
Mol_id: 1; Molecule: Alkaline Phosphatase; Chain: A, B;
Ec: 3.1.3.1; Engineered: Yes; Mutation: D153g;
Heterogen: Zn; Heterogen: Mg; Other_details: Mutant With
4-Fold Increased Activity And Weaker Mg Binding
>gi|1311180|pdb|1AJD|A Chain A, Mol_id: 1; Molecule:
Alkaline Phosphatase Intermediate Ii Of Holo Enzyme;
Chain: A, B; Ec: 3.1.3.1; Engineered: Yes; Mutation:
D153g; Heterogen: Zn; Heterogen: Mg; Other_details:
Mutant With 4-Fold Increased Activity And Weaker Mg
Binding, Str> >gi|1311181|pdb|1AJD|B Chain B, Mol_id: 1;
Molecule: Alkaline Phosphatase Intermediate Ii Of Holo
Enzyme; Chain: A, B; Ec: 3.1.3.1; Engineered: Yes;
Mutation: D153g; Heterogen: Zn; Heterogen: Mg;
Other_details: Mutant With 4-Fold Increased Activity And
Weaker Mg Binding, Str> >gi|1311182|pdb|1AJC|A Chain A,
Mol_id: 1; Molecule: Alkaline Phosphatase; Chain: A, B;
Ec: 3.1.3.1; Engineered: Yes; Mutation: D153g;
Heterogen: Zn; Heterogen: Mg; Other_details: Mutant With
4-Fold Increased Activity And Weaker Mg Binding
>gi|1311186|pdb|1AJA|A Chain A, Mol_id: 1; Molecule:
Alkaline Phosphatase; Chain: A, B; Ec: 3.1.3.1;
Engineered: Yes; Mutation: D153g; Other_details: Apo
Enzyme >gi|1311187|pdb|1AJA|B Chain B, Mol_id: 1;
Molecule: Alkaline Phosphatase; Chain: A, B; Ec:
3.1.3.1; Engineered: Yes; Mutation: D153g;
Other_details: Apo Enzyme
Length = 449
Score = 138 bits (343), Expect = 2e-31
Identities = 107/336 (31%), Positives = 157/336 (45%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQGA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ AE GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREQAEARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>gb|AAF53795.1| (AE003662) CG16771 gene product [Drosophila melanogaster]
Length = 596
Score = 137 bits (341), Expect = 3e-31
Identities = 108/332 (32%), Positives = 154/332 (45%), Gaps = 67/332 (20%)
Query: 31 RNVIILIGDGMGFSQLQLTKLV----YGHLNMEDFPYTGIELTDSLSGEVTDSAAAGTAI 86
+NVI+ +GDGMG + + +++ G L E FP G+ T +V DS + TA+
Sbjct: 134 KNVILFVGDGMGPNTVTAARILGVKEEGLLRWEQFPDMGLLKTYCADKQVPDSFSTATAL 193
Query: 87 ATGVKT------YNRMISTTNVTGKLV---NLTTLLEIAQMLGKATGLVTTTRITHATPA 137
GVK + + N + L ++ T+L+ AQ+ G TG VTTTR+THATPA
Sbjct: 194 FGGVKVNYETGGVDANVPLGNCSASLKEDHHVQTILKWAQVDGMRTGFVTTTRVTHATPA 253
Query: 138 VFASHVPDRDMEEE--------------IARQLILH----NVTVLMGGGREKFSEEVL-- 177
+HVPDR E E IARQLI ++ V+MGGGR+ +V
Sbjct: 254 ALYAHVPDRRWECESGMPAEAQGQGCMDIARQLIEQPTGQSINVIMGGGRQMLVSDVTGS 313
Query: 178 -------------------------KLAEDYGYSIVYTREDLEKVKDGKV---LGLFAEG 209
K E +++V +L + V LG+FA G
Sbjct: 314 AADPLDTWAGQSKDGRDLIRDWRLKKADEGVSHAVVQNNRELWNLNGQDVDYVLGIFANG 373
Query: 210 HLPYVLDRSEEDV---SLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
HL Y +R D SL MT KA+E+L + GF L++E G ID A H + ++E
Sbjct: 374 HLMYDHERDRSDAGMPSLSNMTLKALEVLGNSDKGFLLVVEAGLIDQAHHRGNARKALSE 433
Query: 267 TKEFDDVVGYVLDYAR---RRGDTLVIVLADH 295
E + V L + + R +TLVIV ADH
Sbjct: 434 VLELNAAVESTLSFLKSSDRLDETLVIVTADH 465
>dbj|BAA92180.1| (AB031538) endoderm-specific alkaline phosphatase [Ciona savignyi]
Length = 570
Score = 137 bits (341), Expect = 3e-31
Identities = 102/332 (30%), Positives = 162/332 (48%), Gaps = 69/332 (20%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVII +GDGMG + + +++ G ++ M+ FP++GI T S++ +V+DSA
Sbjct: 52 KNVIIFVGDGMGITTITSGRILKGQVSGTSGEETKLAMDKFPFSGISKTYSVNKQVSDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV---------NLTTLLEIAQMLGKATGLVTTTRI 131
+ TA TGVKT + ++ T + + +T++L A+ GK+ G VTTTRI
Sbjct: 112 STATAFLTGVKTNDFILGLTGSAQRGICKGSIDENNIVTSVLIEAKNAGKSAGFVTTTRI 171
Query: 132 THATPAVFASHVPDR------DMEEE--------IARQLI--LHNVTVLMGGGREKFSEE 175
HATP +H P+R D+ EE +A+Q I H TV +GGGR+ F
Sbjct: 172 NHATPGATYAHTPERMWYGDADLTEEAKANGCKDVAQQFIDNSHLFTVALGGGRQYFRPN 231
Query: 176 VLKLAEDY---------GYSIVYTREDLEKVKDGK--------------------VLGLF 206
+ E+Y G ++ + ++ + + +LGLF
Sbjct: 232 TTQ-DEEYPNKTNARLDGQDLIEQWKQIQLQQGNRAAYVWNATEFAGINPDNTDSLLGLF 290
Query: 207 AEGHLPYVLDRSEE---DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASI 263
+ Y RS + + SL EMT KAI +L+KN G+ L++EGGRIDH H +
Sbjct: 291 QPKDMHYEAHRSGDVAGEPSLSEMTAKAISLLKKNEEGYILLVEGGRIDHGHHEGNAYLA 350
Query: 264 VAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ + FDD + + +T++IV ADH
Sbjct: 351 LHDLVAFDDAIDTAVQMT-SDDETMLIVTADH 381
>pir||PAECA alkaline phosphatase (EC 3.1.3.1) precursor - Escherichia coli
Length = 471
Score = 137 bits (341), Expect = 3e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 60 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 119
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 120 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 176
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 177 TPAALVAHVTSRKCYGPSATSQKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 236
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 237 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 296
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 297 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 356
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 357 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 392
>sp|P00634|PPB_ECOLI ALKALINE PHOSPHATASE PRECURSOR (APASE) >gi|147225|gb|AAA83893.1|
(M13345) alkaline phosphatase [Escherichia coli]
>gi|147231|gb|AAA24364.1| (M29664) alkaline phosphatase
precursor (EC 3.1.3.1) [Escherichia coli]
>gi|147233|gb|AAA24365.1| (M29665) alkaline phosphatase
precursor (EC 3.1.3.1) [Escherichia coli]
>gi|581187|emb|CAA28257.1| (X04586) alkaline phosphatase
[Escherichia coli] >gi|1657579|gb|AAB18107.1| (U73857)
alkaline phosphatase precursor [Escherichia coli]
Length = 471
Score = 137 bits (341), Expect = 3e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 60 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 119
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 120 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 176
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 177 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 236
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 237 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 296
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 297 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 356
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 357 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 392
>pir||I79530 alkaline phosphatase (EC 3.1.3.1) precursor - Escherichia coli
(strain RM202I) >gi|147243|gb|AAA24370.1| (M29670)
alkaline phosphatase precursor (EC 3.1.3.1) [Escherichia
coli]
Length = 471
Score = 137 bits (341), Expect = 3e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 60 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 119
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 120 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 176
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 177 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 236
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVLD----- 216
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 237 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWQGPKAT 296
Query: 217 -----------------RSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 297 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 356
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 357 PCGAIGETVDLDEAVQRALEFAKKDGNTLVIVTADH 392
>gb|AAB60529.1| (U25548) alkaline phosphatase [synthetic construct]
Length = 461
Score = 137 bits (341), Expect = 3e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 50 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 109
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 110 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 166
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 167 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 226
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 227 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 286
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 287 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 346
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 347 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 382
>gb|AAC53686.1| (U50975) phoA gene product [Cloning vector pFW_phoA1]
>gi|1277130|gb|AAC53688.1| (U50976) phoA gene product
[Cloning vector pFW_phoA3] >gi|1277139|gb|AAC53693.1|
(U50980) phoA gene product [Cloning vector pFW_phoA2]
Length = 443
Score = 137 bits (341), Expect = 3e-31
Identities = 114/388 (29%), Positives = 175/388 (44%), Gaps = 74/388 (19%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 32 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 91
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 92 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 148
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 149 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 208
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 209 ATAGEWQGKTLREQAQARGYQLVNDAASLNAVTEANQQKPLLGLFADGNMPVRWLGPKAT 268
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 269 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 328
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRID 319
+ ET + D+ V L++A++ G+TLVIV ADH + + ++ D
Sbjct: 329 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQVVAPDTKAPGLTQALNTKD 388
Query: 320 ASIEEMSKEIKSGGDIRDVIRRHTGLEL 347
++ MS G+ + + HTG +L
Sbjct: 389 GAVMVMSY-----GNSEESSQEHTGSQL 411
>pdb|1ALI|A Chain A, Alkaline Phosphatase Mutant (H412n) >gi|1311178|pdb|1ALI|B
Chain B, Alkaline Phosphatase Mutant (H412n)
>gi|1311175|pdb|1ALJ|A Chain A, Alkaline Phosphatase
Mutant (H412n) >gi|1311176|pdb|1ALJ|B Chain B, Alkaline
Phosphatase Mutant (H412n)
Length = 449
Score = 137 bits (341), Expect = 3e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>pdb|1HQA|A Chain A, Alkaline Phosphatase (H412q) >gi|1421391|pdb|1HQA|B Chain
B, Alkaline Phosphatase (H412q)
Length = 449
Score = 137 bits (341), Expect = 3e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>gb|AAC73486.1| (AE000145) alkaline phosphatase [Escherichia coli K12]
Length = 494
Score = 137 bits (341), Expect = 3e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 83 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 142
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 143 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 199
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 200 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 259
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 260 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 319
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 320 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 379
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 380 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 415
>gb|AAF80181.1|AF247169_1 (AF247169) alkaline phosphatase [Expression vector pSB2991]
Length = 477
Score = 137 bits (341), Expect = 3e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 66 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 125
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 126 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 182
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 183 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 242
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 243 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 302
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 303 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 362
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 363 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 398
>pdb|1ED8|A Chain A, Structure Of E. Coli Alkaline Phosphatase Inhibited By The
Inorganic Phosphate At 1.75a Resolution
>gi|10835667|pdb|1ED8|B Chain B, Structure Of E. Coli
Alkaline Phosphatase Inhibited By The Inorganic
Phosphate At 1.75a Resolution >gi|10835668|pdb|1ED9|A
Chain A, Structure Of E. Coli Alkaline Phosphatase
Without The Inorganic Phosphate At 1.75a Resolution
>gi|10835669|pdb|1ED9|B Chain B, Structure Of E. Coli
Alkaline Phosphatase Without The Inorganic Phosphate At
1.75a Resolution
Length = 449
Score = 137 bits (341), Expect = 3e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>pir||I79526 alkaline phosphatase (EC 3.1.3.1) precursor - Escherichia coli
(strain RM217T) >gi|147235|gb|AAA24366.1| (M29666)
alkaline phosphatase precursor (EC 3.1.3.1) [Escherichia
coli]
Length = 471
Score = 136 bits (340), Expect = 4e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 60 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 119
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 120 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 176
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 177 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 236
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 237 ATAGEWQGKTLREQAQARGYQLVNDAASLNAVTEANQQKPLLGLFADGNMPVRWLGPKAT 296
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 297 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 356
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 357 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 392
>pir||I79529 alkaline phosphatase (EC 3.1.3.1) precursor - Escherichia coli
(strain RM224H) >gi|147241|gb|AAA24369.1| (M29669)
alkaline phosphatase precursor (EC 3.1.3.1) [Escherichia
coli]
Length = 471
Score = 136 bits (339), Expect = 6e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 60 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 119
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 120 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 176
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 177 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 236
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 237 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 296
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 297 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 356
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 357 PCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH 392
>pdb|1ALH|A Chain A, Alkaline Phosphatase (E.C.3.1.3.1) Mutant With Asp 369
Replaced By Asn (D369n) >gi|999954|pdb|1ALH|B Chain B,
Alkaline Phosphatase (E.C.3.1.3.1) Mutant With Asp 369
Replaced By Asn (D369n)
Length = 446
Score = 136 bits (339), Expect = 6e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 35 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 94
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 95 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 151
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 152 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 211
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ AE GY +V L V + +LGLFA+G++P
Sbjct: 212 ATAGEWQGKTLREQAEARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 271
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 272 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 331
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV A+H
Sbjct: 332 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTANH 367
>dbj|BAA13488.1| (D87872) HrES-AP [Halocynthia roretzi]
Length = 604
Score = 136 bits (339), Expect = 6e-31
Identities = 104/329 (31%), Positives = 162/329 (48%), Gaps = 65/329 (19%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGMG S + +++ G + ME FP+ + T S++ +V DSA
Sbjct: 59 KNVILFLGDGMGVSTVTAGRILKGQIRGESGEETKLAMEQFPHAALSKTYSVNKQVADSA 118
Query: 81 AAGTAIATGVKT------YNRMISTTNVTGKLVN-LTTLLEIAQMLGKATGLVTTTRITH 133
+ TA GVKT N + N N + ++L + GK+TG+VTTT++ H
Sbjct: 119 STATAYLCGVKTNYYTIGLNAKVVYNNCQSSKGNEVDSILVDSFKAGKSTGIVTTTQLGH 178
Query: 134 ATPAVFASH-----------VPDRDMEEE---IARQLI--LHNVTVLMGGGRE------- 170
ATP +H +PD E E I RQ H +TV + GGR
Sbjct: 179 ATPGGAYAHSASRKWINDADLPDEAKENECRDITRQFYDNSHMITVALAGGRADMIPVTS 238
Query: 171 ---KFSEEVLKLAE-------------DYGY--SIVYTREDLEKV---KDGKVLGLFAEG 209
++S++ K + D G+ + V+ + + V K ++LGLF
Sbjct: 239 RDPEYSDQSGKREDGMNLINMWKKKMHDNGFEPAYVWNKTEFRNVNVEKTDRLLGLFEPM 298
Query: 210 HLPYVLDRSEE---DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
++ Y +R ++ + SL EMT+KAI+IL+KN NG+FL++EGGRIDH H+N + +
Sbjct: 299 NMQYDANRLKDGSGEPSLAEMTEKAIQILQKNTNGYFLLVEGGRIDHGHHSNAAYLALHD 358
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADH 295
FDD + ++ DTL+I ADH
Sbjct: 359 VVAFDDAIEKAVEMT-SEDDTLIIATADH 386
>gb|AAF50765.1| (AE003565) CG10592 gene product [Drosophila melanogaster]
Length = 524
Score = 136 bits (338), Expect = 7e-31
Identities = 108/330 (32%), Positives = 157/330 (46%), Gaps = 70/330 (21%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----MEDFPYTGIELTDSLSGEVTDSAAAGTAI 86
+NVI+ +GDGM + T+ G N E FPY G+ T +++ DSA TA
Sbjct: 76 KNVILFLGDGMSVHTIAATRAFMGDSNKQVFFEKFPYLGLSKTYAVNERTPDSANTATAY 135
Query: 87 ATGVKTYNRMISTTN-------VTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPAVF 139
TGVK I VT ++ ++ + AQ GK GLVTT R+THA+PA
Sbjct: 136 LTGVKANYGTIGVNAQVQRGDCVTNSSSHVQSIGQWAQEAGKWAGLVTTARVTHASPAGV 195
Query: 140 ASHVPDR---------------DMEEEIARQLIL----HNVTVLMGGGREKFSEE----- 175
+HV +R D+ +IARQL+ + V+MGGGR F ++
Sbjct: 196 YAHVSERNWEHDGEIISSKCSPDVNTDIARQLVEWPVGQELRVIMGGGRSYFRDQSQHDE 255
Query: 176 --VLKLAEDYGYSIVYTREDLEKVK--DGK------------------VLGLFAEGHLPY 213
V L D G ++ +++K + +GK +LGLF HLPY
Sbjct: 256 TGVSGLRTD-GRDLISDWREIKKQQGAEGKYVWSLKGLKETDISKTDYLLGLFDTSHLPY 314
Query: 214 VLDR-----SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETK 268
DR + + SL +MT+ AI++L KN G+FL +E GRID A HA + +T+
Sbjct: 315 HGDRRRTHYEDAEPSLSDMTEAAIKMLSKNEEGYFLFVESGRIDMAHHATKAMKALEDTE 374
Query: 269 EFDDVVGYVLDYARR---RGDTLVIVLADH 295
EF V + AR+ DTL++V +DH
Sbjct: 375 EFAAAV----ELARKMTDEEDTLIVVTSDH 400
>pir||I79527 alkaline phosphatase (EC 3.1.3.1) precursor - Escherichia coli
(strain RM45E) >gi|147237|gb|AAA24367.1| (M29667)
alkaline phosphatase precursor (EC 3.1.3.1) [Escherichia
coli]
Length = 471
Score = 136 bits (338), Expect = 7e-31
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 60 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 119
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 120 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 176
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 177 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 236
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 237 ATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKPLLGLFADGNMPVRWLGPKAT 296
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 297 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 356
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 357 PCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH 392
>gi|6671533 alkaline phosphatase 2, liver; Tissue Non-Specific Alkaline
Phosphatase [Mus musculus]
>gi|130750|sp|P09242|PPBT_MOUSE ALKALINE PHOSPHATASE,
TISSUE-NONSPECIFIC ISOZYME PRECURSOR (AP-TNAP) (TNSALP)
>gi|200346|gb|AAA39928.1| (J02980) alkaline phosphatase
[Mus musculus]
Length = 524
Score = 136 bits (338), Expect = 7e-31
Identities = 102/332 (30%), Positives = 153/332 (45%), Gaps = 71/332 (21%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGMG S + +++ G L+ M+ FP+ + T + + +V DSA
Sbjct: 52 KNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
TA GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGREKFSEEVL 177
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR+
Sbjct: 172 ATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYP--- 228
Query: 178 KLAEDYGYSI------------------------------VYTREDLEKVKDGKV---LG 204
K D Y + V+ R +L + +V LG
Sbjct: 229 KNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLG 288
Query: 205 LFAEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASI 263
LF G + Y L+R+ D SL EM + A+ IL KN GFFL++EGGRIDH H
Sbjct: 289 LFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQA 348
Query: 264 VAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ E E D +G ++ DTL +V ADH
Sbjct: 349 LHEAVEMDQAIGKAGAMTSQK-DTLTVVTADH 379
>gb|AAC33184.1| (U94950) modified E.coli alkaline phosphatase with N-terminal
PelB-leader and C-terminal hexahistidine tag [synthetic
construct]
Length = 491
Score = 136 bits (338), Expect = 7e-31
Identities = 119/429 (27%), Positives = 188/429 (43%), Gaps = 79/429 (18%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 73 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 132
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VT SAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 133 YVTSSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 189
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 190 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 249
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 250 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 309
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 310 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 369
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRID 319
+ ET + D+ V L++A++ G+TLVIV ADH + + ++ D
Sbjct: 370 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKD 429
Query: 320 ASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
++ MS G+ + + HTG +L + ++ N L + + K
Sbjct: 430 GAVMVMSY-----GNSEEDSQEHTGSQL-----RIAAYGPHAANVVGLTDQTDLFYTMKA 479
Query: 380 GVGFVSHKH 388
+G ++H H
Sbjct: 480 ALGDIAHHH 488
>pdb|1ELX|A Chain A, E. Coli Alkaline Phosphatase Mutant (S102a)
>gi|3212517|pdb|1ELX|B Chain B, E. Coli Alkaline
Phosphatase Mutant (S102a)
Length = 449
Score = 136 bits (338), Expect = 7e-31
Identities = 105/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTD+AA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDAAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>pir||A40172 alkaline phosphatase (EC 3.1.3.1) precursor - mouse
Length = 524
Score = 136 bits (338), Expect = 7e-31
Identities = 102/332 (30%), Positives = 153/332 (45%), Gaps = 71/332 (21%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGMG S + +++ G L+ M+ FP+ + T + + +V DSA
Sbjct: 52 KNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSA 111
Query: 81 AAGTAIATGVKTYNRMISTTNVTGKLV-------NLTTLLEIAQMLGKATGLVTTTRITH 133
TA GVK + + T + +T++L A+ GK+ G+VTTTR+ H
Sbjct: 112 GTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNH 171
Query: 134 ATPAVFASHVPDRDME--------------EEIARQLI--LHNVTVLMGGGREKFSEEVL 177
ATP+ +H DRD ++IA QL+ + ++ V+MGGGR+
Sbjct: 172 ATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYP--- 228
Query: 178 KLAEDYGYSI------------------------------VYTREDLEKVKDGKV---LG 204
K D Y + V+ R +L + +V LG
Sbjct: 229 KNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLG 288
Query: 205 LFAEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASI 263
LF G + Y L+R+ D SL EM + A+ IL KN GFFL++EGGRIDH H
Sbjct: 289 LFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQA 348
Query: 264 VAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ E E D +G ++ DTL +V ADH
Sbjct: 349 LHEAVEMDQAIGKAGAMTSQK-DTLTVVTADH 379
>gb|AAA24363.1| (M29663) alkaline phosphatase precursor (EC 3.1.3.1) [Escherichia
coli]
Length = 471
Score = 135 bits (337), Expect = 1e-30
Identities = 106/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 60 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 119
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 120 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKASGLATGNVSTAELQDA 176
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 177 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 236
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 237 ATAGEWQGKTLREQAQARGYQLVNDAASLNAVTEANQQKPLLGLFADGNMPVRWLGPKAT 296
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 297 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 356
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 357 PCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH 392
>pdb|1ELZ|A Chain A, E. Coli Alkaline Phosphatase Mutant (S102g)
>gi|3212521|pdb|1ELZ|B Chain B, E. Coli Alkaline
Phosphatase Mutant (S102g)
Length = 449
Score = 135 bits (337), Expect = 1e-30
Identities = 105/336 (31%), Positives = 156/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTD AA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDGAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>pdb|1B8J|A Chain A, Alkaline Phosphatase Complexed With Vanadate
>gi|4558083|pdb|1B8J|B Chain B, Alkaline Phosphatase
Complexed With Vanadate
Length = 449
Score = 135 bits (337), Expect = 1e-30
Identities = 105/336 (31%), Positives = 156/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTD AA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDXAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>pdb|1URB|A Chain A, Alkaline Phosphatase (N51mg) >gi|1633108|pdb|1URB|B Chain
B, Alkaline Phosphatase (N51mg) >gi|1633109|pdb|1URA|A
Chain A, Alkaline Phosphatase (D51zn)
>gi|1633110|pdb|1URA|B Chain B, Alkaline Phosphatase
(D51zn)
Length = 446
Score = 135 bits (336), Expect = 1e-30
Identities = 105/336 (31%), Positives = 157/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIG+GMG S++ + ++ P TG +L+ +
Sbjct: 35 SDKPAKNIILLIGNGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 94
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 95 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 151
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 152 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 211
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 212 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 271
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 272 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 331
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 332 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 367
>pdb|1ELY|A Chain A, E. Coli Alkaline Phosphatase Mutant (S102c)
>gi|3212519|pdb|1ELY|B Chain B, E. Coli Alkaline
Phosphatase Mutant (S102c)
Length = 449
Score = 135 bits (336), Expect = 1e-30
Identities = 105/336 (31%), Positives = 156/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTD AA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDCAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>gi|6671537 alkaline phosphatase 5 [Mus musculus]
>gi|130742|sp|P24823|PPBE_MOUSE ALKALINE PHOSPHATASE,
EMBRYONIC PRECURSOR (EAP) >gi|192977|gb|AAA37531.1|
(M61704) embryonic alkaline phosphatase [Mus musculus]
Length = 529
Score = 134 bits (335), Expect = 2e-30
Identities = 125/477 (26%), Positives = 208/477 (43%), Gaps = 89/477 (18%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N++IL+GDGMG S + T+++ G L M+ FP+ + T + ++
Sbjct: 49 TSAKNLVILMGDGMGVSTVTATRILKGQQQGLSGPETQLAMDRFPHMALSKTYNTDKQIP 108
Query: 78 DSAAAGTAIATGVKTYNRMISTTNVTG-KLVNLT------TLLEIAQMLGKATGLVTTTR 130
DSA GTA GVKT ++I + N T +++ A+ GK+ G+VTTT
Sbjct: 109 DSAGTGTAFLCGVKTNMKVIGLSAAARFNQCNTTWGNEVVSVMHRAKKAGKSVGVVTTTS 168
Query: 131 ITHATPAVFASHVPDR--------------DMEEEIARQLILH-NVTVLMGGGR------ 169
+ HA+PA +H +R D ++I+ QLI + ++ V++GGGR
Sbjct: 169 VQHASPAGTYAHTVNRGWYSDAQMPASALQDGCKDISTQLISNMDIDVILGGGRKFMFPK 228
Query: 170 ----EKFSEEVLK--------------LAEDYGYSIVYTREDLEKVKDGK----VLGLFA 207
+++ + + LA+ G V+ R +L + + ++GLF
Sbjct: 229 GTPDQEYPTDTKQAGTRLDGRNLVQEWLAKHQGARYVWNRSELIQASLNRSVTHLMGLFE 288
Query: 208 EGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
+ Y + R +D SL EMT+ A+ +L +NP GF+L +EGGRIDH H + E
Sbjct: 289 PNDMKYEIHRDPAQDPSLAEMTEVAVRMLSRNPKGFYLFVEGGRIDHGHHETVAYRALTE 348
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
FD V D DT+++V ADH H + ASI ++
Sbjct: 349 AVMFDSAVDKA-DIRTSEQDTMILVTADH-----------SHVFSFGGYTQRGASIFGLA 396
Query: 327 K-EIKSGGDIRDVIRRH-TGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFV 384
+ + G ++ + G +L + ++ E ++S Y ++ V
Sbjct: 397 PFKAEDGKSFTSILYGNGPGYKLHNGARADVTEEESSNPTY----------QQQACVPLS 446
Query: 385 SHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIFGDRSISFTIKGVSKIKG 441
S H+GE V + A GP A H V IA +M F +T G++ G
Sbjct: 447 SETHSGEDVAIFARGPQAH----LVHGVQEQNYIAHVMAFAACLEPYTDCGLASPAG 499
>sp|P09401|STRK_STRGR STREPTOMYCIN-6-PHOSPHATE PHOSPHATASE PRECURSOR
>gi|80804|pir||S17780 strK protein - Streptomyces
griseus >gi|1621273|emb|CAA68522.1| (Y00459)
streptomycin-6-phosphate phosphatase [Streptomyces
griseus]
Length = 449
Score = 134 bits (335), Expect = 2e-30
Identities = 111/336 (33%), Positives = 152/336 (45%), Gaps = 67/336 (19%)
Query: 25 ASPSGVRNVIILIGDGMGFSQLQLTKL----VYGHLNMEDFPYTGIELTDSLSGE----- 75
+ P+ R+VI+LIGDGMG +++ + G L M+ +G T ++
Sbjct: 36 SGPAPARSVILLIGDGMGDAEITAARNYSVGAAGRLAMDTLDASGRRTTYAVDERGRPVY 95
Query: 76 VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHAT 135
VTDSAA TA ATG +T N +S ++ T + + TLLE+A+ G ATG VTT + AT
Sbjct: 96 VTDSAAGATAWATGRRTVNGRVSKSHDTDR--PMPTLLELARDRGYATGSVTTASVADAT 153
Query: 136 PAVFASHVPDR------DM------------EEEIARQLILHNVTVLMGGGREKFSE--- 174
PA +HV DR DM E IA Q + VL+GGG + F++
Sbjct: 154 PAALTAHVTDRSCKGPADMAACPADTRAGGGEGSIAEQTVAARPDVLLGGGADHFAQTVT 213
Query: 175 -------EVLKLAEDYGYSIVYTREDLEKVKDGK-VLGLFAEGHLPYVL----------- 215
V + A GY +V R L + G+ VLGLFA ++P
Sbjct: 214 DGPFRGRTVTQQARAAGYQVVRDRTQLAAARPGRPVLGLFAPEYVPVEWTGPPAAPGGTA 273
Query: 216 ---------DRSEEDVSLLEMTKKAIEILEKNPN-------GFFLMIEGGRIDHACHAND 259
R L E T+ A+++L + GFFL +EG ID H D
Sbjct: 274 PQRCATRNPGRPAGTPDLAESTRAALDLLTARAHHRGAPGRGFFLQVEGASIDDRAHEAD 333
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET FD V LD+A R TLVIV ADH
Sbjct: 334 PCGQLGETLAFDRAVAAALDHAERHPRTLVIVTADH 369
>pir||I79528 alkaline phosphatase (EC 3.1.3.1) precursor - Escherichia coli
(strain RM70B) >gi|147239|gb|AAA24368.1| (M29668)
alkaline phosphatase precursor (EC 3.1.3.1) [Escherichia
coli]
Length = 471
Score = 134 bits (333), Expect = 3e-30
Identities = 105/336 (31%), Positives = 156/336 (46%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 60 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 119
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTDSAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 120 YVTDSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 176
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 177 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 236
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVLD----- 216
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 237 ATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKPLLGLFADGNMPVRWQGPKAT 296
Query: 217 -----------------RSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 297 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 356
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+ LVIV ADH
Sbjct: 357 PCGQIGETVDLDEAVQRALEFAKKDGNALVIVTADH 392
>gb|AAF36717.1|AF227507_1 (AF227507) intestinal alkaline phosphatase-I [Rattus norvegicus]
Length = 540
Score = 133 bits (332), Expect = 4e-30
Identities = 121/458 (26%), Positives = 193/458 (41%), Gaps = 85/458 (18%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVT 77
+ +N+I+ +GDGMG + T+++ G L M+ FP+T + T ++ +V
Sbjct: 51 TSAKNLILFLGDGMGVPTVTATRILKGQLGGHLGPETPLAMDHFPFTALSKTYNVDRQVP 110
Query: 78 DSAAAGTAIATGVKTYNRMISTTNVTG-KLVNLT------TLLEIAQMLGKATGLVTTTR 130
DSA TA GVK + I + N T +++ A+ GK+ G+VTTTR
Sbjct: 111 DSAGTATAYLCGVKANYKTIGVSAAARFNQCNSTFGNEVFSVMHRAKKAGKSVGVVTTTR 170
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGREKF--- 172
+ HA+PA +H +RD ++IA QLI + ++ V++GGGR+
Sbjct: 171 VQHASPAGTYAHTVNRDWYSDADMPSSALQEGCKDIATQLISNMDIDVILGGGRKSMFPK 230
Query: 173 -------------------SEEVLK--LAEDYGYSIVYTREDLEKVKDG----KVLGLFA 207
S +++ LA+ G V+ RE L + +++GLF
Sbjct: 231 GTPDPEYPGDSDQSGVRLDSRNLVEEWLAKYQGTRYVWNREQLMRASQDPAVTRLMGLFE 290
Query: 208 EGHLPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
+ Y ++R+ D SL EMT+ A+ +L +NP GF+L +EGGRID HA + E
Sbjct: 291 PTEMKYDVNRNASADPSLAEMTEVAVRLLSRNPQGFYLFVEGGRIDQGHHAGTAYLALTE 350
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
FD + + DTL ++ ADH + G I + ++A +
Sbjct: 351 AAMFDSAIEKASQLTNEK-DTLTLITADH-SHVFAFGGYTLRGTSIFGLAPLNAQDGKSY 408
Query: 327 KEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSH 386
I G V+ +TD E ++ ++ V S
Sbjct: 409 TSILYGNGPGYVLNSGNRPNVTDAESGDVN------------------YKQQAAVPLSSE 450
Query: 387 KHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIF 424
H GE V + A GP A H V IA +M F
Sbjct: 451 THGGEDVAIFARGPQAH----LVHGVQEQNYIAHVMAF 484
>emb|CAA37374.1| (X53279) protein precursor (AA -19 to 513) [Homo sapiens]
Length = 532
Score = 132 bits (329), Expect = 8e-30
Identities = 126/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T S+ V
Sbjct: 50 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 110 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 169
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 170 VQHASPAGAYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPM 229
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 230 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELMQASLDPSVTHLMGLFE 289
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 290 PGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 349
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 350 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHVFSFGGYPLRGSSIFGLA 397
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 398 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 442
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 443 AVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 482
>pdb|1HJK|A Chain A, Alkaline Phosphatase Mutant H331q >gi|2624849|pdb|1HJK|B
Chain B, Alkaline Phosphatase Mutant H331q
Length = 449
Score = 132 bits (329), Expect = 8e-30
Identities = 104/336 (30%), Positives = 155/336 (45%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 38 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 97
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VTD AA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 98 YVTDXAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 154
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 155 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 214
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVL------ 215
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 215 ATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKAT 274
Query: 216 ----------------DRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID A +
Sbjct: 275 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDQAAN 334
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+TLVIV ADH
Sbjct: 335 PCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH 370
>pir||S12076 alkaline phosphatase (EC 3.1.3.1) precursor, placental-like - human
Length = 532
Score = 132 bits (328), Expect = 1e-29
Identities = 126/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T S+ V
Sbjct: 50 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 110 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 169
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 170 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPM 229
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 230 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFE 289
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 290 PGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 349
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 350 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHVFSFGGYPLRGSSIFGLA 397
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 398 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 442
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 443 AVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 482
>gb|AAC32393.1| (AF083070) alkaline phosphatase [Expression vector pSKAP/S]
Length = 495
Score = 131 bits (327), Expect = 1e-29
Identities = 104/336 (30%), Positives = 155/336 (45%), Gaps = 69/336 (20%)
Query: 26 SPSGVRNVIILIGDGMGFSQLQLTKLVYGHLN-----MEDFPYTGIELTDSLSGE----- 75
S +N+I+LIGDGMG S++ + ++ P TG +L+ +
Sbjct: 73 SDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPD 132
Query: 76 -VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHA 134
VT SAA+ TA +TGVKTYN + ++ K + T+LE+A+ G ATG V+T + A
Sbjct: 133 YVTSSAASATAWSTGVKTYNGALGV-DIHEK--DHPTILEMAKAAGLATGNVSTAELQDA 189
Query: 135 TPAVFASHVPDRDM-------------------EEEIARQLILHNVTVLMGGGREKFSEE 175
TPA +HV R + I QL+ V +GGG + F+E
Sbjct: 190 TPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAET 249
Query: 176 VL----------KLAEDYGYSIVYTREDLEKVKDGK----VLGLFAEGHLPYVLD----- 216
+ A+ GY +V L V + +LGLFA+G++P
Sbjct: 250 ATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKPLLGLFADGNMPVRWQGPKAT 309
Query: 217 -----------------RSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHAND 259
R++ +L +MT KAIE+L KN GFFL +EG ID HA +
Sbjct: 310 YHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAAN 369
Query: 260 VASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ ET + D+ V L++A++ G+ LVIV ADH
Sbjct: 370 PCGQIGETVDLDEAVQRALEFAKKDGNALVIVTADH 405
>gb|AAF64515.1| (AF250845) alkaline phosphatase [Canis familiaris]
Length = 446
Score = 131 bits (327), Expect = 1e-29
Identities = 100/329 (30%), Positives = 157/329 (47%), Gaps = 62/329 (18%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVT 77
+ V+N+I+ +GDGMG + T+++ G +N M+ FPY + T ++ +V
Sbjct: 27 TAVKNLILFLGDGMGVPTVTATRILKGQINDNLGPETPLAMDQFPYLALSKTYNVDRQVP 86
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DSA TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 87 DSAGTATAYLCGVKANYQTIGVSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 146
Query: 131 ITHATPAVFASHVPDR--------------DMEEEIARQLILH-NVTVLMGGGREKF--- 172
+ HA+PA +HV +R D ++IA+QLI + + V++GGGR+
Sbjct: 147 VQHASPAGTYAHVVNRNWYSDANMPAKALEDGCQDIAQQLISNMEIDVILGGGRKYMFPK 206
Query: 173 -----------SEEVLKL----------AEDYGYSIVYTREDL-EKVKDGKV---LGLFA 207
+ ++L A+ G V+ R L + +D V +GLF
Sbjct: 207 GTPDPEYPTDAKQNGIRLDGRNLVQEWQAKYQGARYVWNRTALIQASQDASVTHLMGLFE 266
Query: 208 EGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G Y + R +D SL+EMT+ A+ +L +NP GF+L +EGGRIDH H + E
Sbjct: 267 PGDTKYDVHRDGIQDPSLMEMTEAALRLLSRNPKGFYLFVEGGRIDHGHHDGTAYLALTE 326
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADH 295
FD + R DTL +V ADH
Sbjct: 327 AVMFDSAIDKAGQLTSER-DTLTLVTADH 354
>sp|P15693|PPBI_RAT ALKALINE PHOSPHATASE, INTESTINAL 1 PRECURSOR (IAP-I)
>gi|91939|pir||S08214 alkaline phosphatase (EC 3.1.3.1),
intestinal type I precursor - rat
>gi|57823|emb|CAA35613.1| (X17611) precursor polypeptide
(AA -20 to 520) [Rattus sp.]
Length = 540
Score = 131 bits (327), Expect = 1e-29
Identities = 121/458 (26%), Positives = 193/458 (41%), Gaps = 85/458 (18%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVT 77
+ +N+I+ +GDGMG + T+++ G L M+ FP+T + T ++ +V
Sbjct: 51 TSAKNLILFLGDGMGVPTVTATRILKGQLGGHLGPETPLAMDHFPFTALSKTYNVDRQVP 110
Query: 78 DSAAAGTAIATGVKTYNRMISTTNVTG-KLVNLT------TLLEIAQMLGKATGLVTTTR 130
DSA TA GVK + I + N T +++ A+ GK+ G+VTTTR
Sbjct: 111 DSAGTATAYLCGVKANYKTIGVSAAARFNQCNSTFGNEVFSVMHRAKKAGKSVGVVTTTR 170
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGREKF--- 172
+ HA+PA +H +RD ++IA QLI + ++ V++GGGR+
Sbjct: 171 VQHASPAGTYAHTVNRDWYSDADMPSSALQEGCKDIATQLISNMDIDVILGGGRKFMFPK 230
Query: 173 -------------------SEEVLK--LAEDYGYSIVYTREDLEKVKDG----KVLGLFA 207
S +++ LA+ G V+ RE L + +++GLF
Sbjct: 231 GTPDPEYPGDSDQSGVRLDSRNLVEEWLAKYQGTRYVWNREQLMQASQDPAVTRLMGLFE 290
Query: 208 EGHLPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
+ Y ++R+ D SL EMT+ A+ +L +NP GF+L +EGGRID HA + E
Sbjct: 291 PTEMKYDVNRNASADPSLAEMTEVAVRLLSRNPQGFYLFVEGGRIDQGHHAGTAYLALTE 350
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
FD + + DTL ++ ADH + G I + ++A +
Sbjct: 351 AVMFDSAIEKASQLTNEK-DTLTLITADH-SHVFAFGGYTLRGTSIFGLAPLNAQDGKSY 408
Query: 327 KEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSH 386
I G V+ +TD E ++ ++ V S
Sbjct: 409 TSILYGNGPGYVLNSGNRPNVTDAESGDVN------------------YKQQAAVPLSSE 450
Query: 387 KHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIF 424
H GE V + A GP A H V IA +M F
Sbjct: 451 THGGEDVAIFARGPQAH----LVHGVQEQNYIAHVMAF 484
>pir||I37991 alkaline phosphatase - human >gi|296798|emb|CAA39425.1| (X55958)
alkaline phosphatase [Homo sapiens]
Length = 532
Score = 131 bits (327), Expect = 1e-29
Identities = 126/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T S+ V
Sbjct: 50 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 110 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVVNRAKKAGKSVGVVTTTR 169
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 170 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPM 229
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 230 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFE 289
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 290 PGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 349
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 350 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHVFSFGGYPLRGSSIFGLA 397
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 398 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 442
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 443 AVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 482
>gb|AAA51704.1| (M21499) intestinal alkaline phosphatase precursor [Homo sapiens]
Length = 528
Score = 131 bits (326), Expect = 2e-29
Identities = 124/455 (27%), Positives = 199/455 (43%), Gaps = 85/455 (18%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+N+I+ +GDG+G + T+++ G N M+ FPY + T ++ +V DSA
Sbjct: 53 KNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSA 112
Query: 81 AAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTRITH 133
A TA GVK + I + T + + +++ A+ GK+ G+VTTTR+ H
Sbjct: 113 ATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQH 172
Query: 134 ATPAVFASHVPDR------DME--------EEIARQLILH-NVTVLMGGGREKF------ 172
A+PA +H +R DM ++IA QLI + ++ V++GGGR+
Sbjct: 173 ASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTP 232
Query: 173 --------SEEVLKL----------AEDYGYSIVYTREDLEKVKDGK----VLGLFAEGH 210
S+ ++L A+ G V+ R +L + + ++GLF G
Sbjct: 233 DPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGD 292
Query: 211 LPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETKE 269
Y + R D SL+EMT+ A+ +L +NP GF+L +EGGRIDH H + E
Sbjct: 293 TKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVM 352
Query: 270 FDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMSKEI 329
FDD + DTL +V ADH + G G+++ SI + S + SK
Sbjct: 353 FDDAIERAGQLTSEE-DTLTLVTADH-SHVFSFG---GYTLRGSSIFGLAPSKAQDSKAY 407
Query: 330 KSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSHKHT 389
S + G ++ E+++ + Y ++ V S H
Sbjct: 408 TS-----TLYGNGPGYVFNSGVRPDVNESESGSPDY----------QQQAAVPLSSETHG 452
Query: 390 GEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIF 424
GE V + A GP A H V +A +M F
Sbjct: 453 GEDVAVFARGPQAH----LVHGVQEQSFVAHVMAF 483
>sp|P19111|PPBI_BOVIN ALKALINE PHOSPHATASE, INTESTINAL PRECURSOR (IAP)
>gi|478841|pir||S30364 alkaline phosphatase (EC 3.1.3.1)
precursor, intestinal - bovine >gi|289416|gb|AAA30571.1|
(L07733) intestinal alkaline phosphatase [Bos taurus]
Length = 533
Score = 131 bits (326), Expect = 2e-29
Identities = 116/458 (25%), Positives = 198/458 (42%), Gaps = 85/458 (18%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVT 77
+ +NVI+ +GDGMG + T+++ G +N M+ FPY + T ++ +V
Sbjct: 50 TAAKNVILFLGDGMGVPTVTATRILKGQMNGKLGPETPLAMDQFPYVALSKTYNVDRQVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DSA TA GVK R I + T + +T+++ A+ GK+ G+VTTTR
Sbjct: 110 DSAGTATAYLCGVKGNYRTIGVSAAARYNQCKTTRGNEVTSVMNRAKKAGKSVGVVTTTR 169
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QL+ + ++ V++GGGR+
Sbjct: 170 VQHASPAGAYAHTVNRNWYSDADLPADAQMNGCQDIAAQLVNNMDIDVILGGGRKYMFPV 229
Query: 171 -----------------KFSEEVLKL--AEDYGYSIVYTREDLEKVKDGK----VLGLFA 207
K + +++ A+ G V+ R L + D ++GLF
Sbjct: 230 GTPDPEYPDDASVNGVRKRKQNLVQAWQAKHQGAQYVWNRTALLQAADDSSVTHLMGLFE 289
Query: 208 EGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
+ Y + + +D +L EMT+ A+ ++ +NP GF+L +EGGRIDH H + + E
Sbjct: 290 PADMKYNVQQDHTKDPTLQEMTEVALRVVSRNPRGFYLFVEGGRIDHGHHDDKAYMALTE 349
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
FD+ + + DTL++V ADH ++ S ++R S
Sbjct: 350 AGMFDNAIAKANELTSEL-DTLILVTADH---------SHVFSFGGYTLRGTSIFGLAPS 399
Query: 327 KEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSH 386
K + S + G L ++ ++ + Y ++ V S
Sbjct: 400 KALDSKSYTSILYGNGPGYALGGGSRPDVNDSTSEDPSY----------QQQAAVPQASE 449
Query: 387 KHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIF 424
H GE V + A GP A H V+ +A +M F
Sbjct: 450 THGGEDVAVFARGPQAH----LVHGVEEETFVAHIMAF 483
>gi|4502065 alkaline phosphatase, placental (Regan isozyme) [Homo sapiens]
>gi|130740|sp|P05188|PPB3_HUMAN ALKALINE PHOSPHATASE,
PLACENTAL TYPE 3 PRECURSOR >gi|178470|gb|AAA51709.1|
(M14170) preplacental alkaline phosphatase (EC 3.1.3.1)
[Homo sapiens]
Length = 535
Score = 131 bits (325), Expect = 2e-29
Identities = 122/463 (26%), Positives = 197/463 (42%), Gaps = 101/463 (21%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDG+G S + +++ G L M+ FPY + T ++ V
Sbjct: 53 TAAKNLIIFLGDGVGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVP 112
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 113 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 172
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 173 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRM 232
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK----DGKVLGLFA 207
++ ++ + LA+ G V+ R +L + ++GLF
Sbjct: 233 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELMRASLDPSVAHLMGLFE 292
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 293 PGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 352
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHE----------TGGLGIGLNYGHSVDIDSIR 316
T FDD + DTL +V ADH GG GL G + D +
Sbjct: 353 TIMFDDAIERAGQLTSEE-DTLSLVTADHSHVFSFGGCPLRGGSIFGLAPGKARDRKAYT 411
Query: 317 RIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIIS 376
+ + G L D ++ E+++ + +Y
Sbjct: 412 VL-------------------LYGNGPGYVLKDGARPDVTESESGSPEY----------R 442
Query: 377 KKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
++ V H GE V + A GP A G + A V+A
Sbjct: 443 QQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 485
>sp|P05187|PPB1_HUMAN ALKALINE PHOSPHATASE, PLACENTAL TYPE 1 PRECURSOR (PLAP-1)
>gi|1070483|pir||PAHUA alkaline phosphatase (EC 3.1.3.1)
precursor, placental - human >gi|178476|gb|AAA51710.1|
(M19159) placental alkaline phosphatase-1 [Homo sapiens]
Length = 535
Score = 131 bits (325), Expect = 2e-29
Identities = 125/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T ++ V
Sbjct: 53 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVP 112
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 113 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 172
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 173 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRM 232
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 233 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFE 292
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 293 PGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 352
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 353 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHVFSFGGYPLRGSSIFGLA 400
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 401 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 445
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 446 AVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 485
>gb|AAA51700.1| (J04948) alkaline phosphatase precursor (EC 3.1.31.) [Homo sapiens]
Length = 532
Score = 131 bits (325), Expect = 2e-29
Identities = 126/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T S+ V
Sbjct: 50 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 110 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 169
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 170 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPM 229
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 230 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFE 289
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 290 PGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 349
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 350 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHLFSFGGYPLRGSSIFGLA 397
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 398 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 442
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 443 AVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 482
>gb|AAA51708.1| (M14169) preplacental alkaline phosphatase type 1 (EC 3.1.3.1)
[Homo sapiens]
Length = 518
Score = 131 bits (325), Expect = 2e-29
Identities = 125/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T ++ V
Sbjct: 36 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVP 95
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 96 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 155
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 156 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRM 215
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 216 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFE 275
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 276 PGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 335
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 336 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHVFSFGGYPLRGSSIFGLA 383
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 384 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 428
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 429 AVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 468
>gb|AAC97139.1| (M13078) alkaline phosphatase precursor [Homo sapiens]
Length = 535
Score = 131 bits (325), Expect = 2e-29
Identities = 125/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T ++ V
Sbjct: 53 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVP 112
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 113 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 172
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 173 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPM 232
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 233 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFE 292
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 293 PGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 352
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 353 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHVFSFGGYPLRGSSIFGLA 400
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 401 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 445
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 446 AVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 485
>gb|AAB64400.1| (U89937) secreted alkaline phosphatase [unidentified cloning
vector] >gi|2190726|gb|AAB64402.1| (U89938) secreted
alkaline phosphatase [unidentified cloning vector]
>gi|2190729|gb|AAB64404.1| (U89939) secreted alkaline
phosphatase [unidentified cloning vector]
>gi|2190732|gb|AAB64406.1| (U89940) secreted alkaline
phosphatase [unidentified cloning vector]
Length = 519
Score = 131 bits (325), Expect = 2e-29
Identities = 125/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T ++ V
Sbjct: 48 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVP 107
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 108 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 167
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 168 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRM 227
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 228 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFE 287
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 288 PGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 347
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 348 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHVFSFGGYPLRGSSIFGLA 395
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 396 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 440
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 441 AVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 480
>sp|P09923|PPBI_HUMAN ALKALINE PHOSPHATASE, INTESTINAL PRECURSOR (IAP)
>gi|1070484|pir||PAHUI alkaline phosphatase (EC 3.1.3.1)
precursor, intestinal - human >gi|178442|gb|AAA98617.1|
(J03930) alkaline phosphatase [Homo sapiens]
Length = 528
Score = 130 bits (324), Expect = 3e-29
Identities = 124/455 (27%), Positives = 199/455 (43%), Gaps = 85/455 (18%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+N+I+ +GDG+G + T+++ G N M+ FPY + T ++ +V DSA
Sbjct: 53 KNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSA 112
Query: 81 AAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTRITH 133
A TA GVK + I + T + + +++ A+ GK+ G+VTTTR+ H
Sbjct: 113 ATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQH 172
Query: 134 ATPAVFASHVPDR------DME--------EEIARQLILH-NVTVLMGGGREKF------ 172
A+PA +H +R DM ++IA QLI + ++ V++GGGR+
Sbjct: 173 ASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTP 232
Query: 173 --------SEEVLKL----------AEDYGYSIVYTREDLEKVKDGK----VLGLFAEGH 210
S+ ++L A+ G V+ R +L + + ++GLF G
Sbjct: 233 DPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGD 292
Query: 211 LPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETKE 269
Y + R D SL+EMT+ A+ +L +NP GF+L +EGGRIDH H + E
Sbjct: 293 TKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVM 352
Query: 270 FDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMSKEI 329
FDD + DTL +V ADH + G G+++ SI + S + SK
Sbjct: 353 FDDAIERAGQLTSEE-DTLTLVTADH-SHVFSFG---GYTLRGSSIFGLAPSKAQDSKAY 407
Query: 330 KSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSHKHT 389
S + G ++ E+++ + Y ++ V S H
Sbjct: 408 TS-----ILYGNGPGYVFNSGVRPDVNESESGSPDY----------QQQAAVPLSSETHG 452
Query: 390 GEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIF 424
GE V + A GP A H V +A +M F
Sbjct: 453 GEDVAVFARGPQAH----LVHGVQEQSFVAHVMAF 483
>gi|4502061 alkaline phosphatase, intestinal [Homo sapiens]
>gi|178432|gb|AAA51703.1| (M15694) adult intestinal
phosphatase (EC 3.1.3.1) [Homo sapiens]
Length = 528
Score = 130 bits (324), Expect = 3e-29
Identities = 125/455 (27%), Positives = 199/455 (43%), Gaps = 85/455 (18%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+N+I+ +GDG+G + T+++ G N M+ FPY + T ++ +V DSA
Sbjct: 53 KNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSA 112
Query: 81 AAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTRITH 133
A TA GVK + I + T + + +++ A+ GK+ G+VTTTR+ H
Sbjct: 113 ATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQH 172
Query: 134 ATPAVFASHVPDR------DME--------EEIARQLILH-NVTVLMGGGREKF------ 172
A+PA +H +R DM ++IA QLI + ++ V++GGGR+
Sbjct: 173 ASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTP 232
Query: 173 --------SEEVLKL----------AEDYGYSIVYTREDLEKVKDGK----VLGLFAEGH 210
S+ ++L A+ G V+ R +L + + ++GLF G
Sbjct: 233 DPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGD 292
Query: 211 LPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETKE 269
Y + R D SL+EMT+ A+ +L +NP GF+L +EGGRIDH H V E
Sbjct: 293 TKYEILRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQAVTEAVM 352
Query: 270 FDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMSKEI 329
FDD + DTL +V ADH + G G+++ SI + S + SK
Sbjct: 353 FDDAIERAGQLTSEE-DTLTLVTADH-SHVFSFG---GYTLRGSSIFGLAPSKAQDSKAY 407
Query: 330 KSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSHKHT 389
S + G ++ E+++ + Y ++ V S H
Sbjct: 408 TS-----ILYGNGPGYVFNSGVRPDVNESESGSPDY----------QQQAAVPLSSETHG 452
Query: 390 GEPVPLLAYGPGAENFVGFKHHVDTAKVIAKLMIF 424
GE V + A GP A H V +A +M F
Sbjct: 453 GEDVAVFARGPQAH----LVHGVQEQSFVAHVMAF 483
>sp|P10696|PPBN_HUMAN ALKALINE PHOSPHATASE, PLACENTAL-LIKE PRECURSOR (NAGAO ISOZYME)
(GERM-CELL ALKALINE PHOSPHATASE) (PLAP-LIKE) (ALP-1)
Length = 532
Score = 130 bits (324), Expect = 3e-29
Identities = 126/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T S+ V
Sbjct: 50 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 110 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVVNRAKKAGKSVGVVTTTR 169
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 170 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPM 229
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 230 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFE 289
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 290 PGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 349
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 350 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHVFSFGGYPLRGSSIFGLA 397
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 398 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 442
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 443 AVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 482
>sp|P06861|PPB2_HUMAN ALKALINE PHOSPHATASE, PLACENTAL PRECURSOR (REGAN ISOZYME)
>gi|178464|gb|AAA51706.1| (M12551) placental alkaline
phosphatase precursor [Homo sapiens]
Length = 530
Score = 129 bits (322), Expect = 5e-29
Identities = 124/463 (26%), Positives = 197/463 (41%), Gaps = 101/463 (21%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T ++ V
Sbjct: 48 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVP 107
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 108 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 167
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 168 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRM 227
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + L E G V+ R +L + D V +GLF
Sbjct: 228 GTPDPEYPDDYSQGGTRLDGKNLVQEWLGERQGARYVWNRTELMQASLDPSVTHLMGLFE 287
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L ++P GFFL +EGGRIDH H + + E
Sbjct: 288 PGDMKYEIHRDSTLDPSLMEMTEAALRLLSRHPRGFFLFVEGGRIDHGHHESRAYRALTE 347
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHE----------TGGLGIGLNYGHSVDIDSIR 316
T FDD + DTL +V ADH G IGL G + D +
Sbjct: 348 TIMFDDAIERAGQLTSEE-DTLSLVTADHSHVFSFGGYPLRGSSFIGLAAGKARDRKAYT 406
Query: 317 RIDASIEEMSKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIIS 376
+ + G L D ++ E+++ + +Y
Sbjct: 407 VL-------------------LYGNGPGYVLKDGARPDVTESESGSPEY----------R 437
Query: 377 KKLGVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
++ V H GE V + A GP A G + A V+A
Sbjct: 438 QQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 480
>gb|AAA98616.1| (J03252) alkaline phosphatase [Homo sapiens]
Length = 532
Score = 129 bits (321), Expect = 7e-29
Identities = 125/460 (27%), Positives = 198/460 (42%), Gaps = 95/460 (20%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+I+ +GDGMG S + +++ G L M+ FPY + T S+ V
Sbjct: 50 TAAKNLIMFLGDGMGVSTVTAARILKGQKKDKLGPETFLAMDRFPYVALSKTYSVDKHVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 110 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVVNRAKKAGKSVGVVTTTR 169
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 170 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPM 229
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDLEKVK-DGKV---LGLFA 207
++ ++ + LA+ G V+ R +L + D V +GLF
Sbjct: 230 GTPDPEYPDDYSQGGTRLDGKNLVQEWLAKHQGARYVWNRTELLQASLDPSVTHLMGLFE 289
Query: 208 EGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H + + E
Sbjct: 290 PGDMKYEIHRDSTLDPSLMEMTEAALLLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTE 349
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMS 326
T FDD + DTL +V ADH H +SI ++
Sbjct: 350 TIMFDDAIERAGQLTSEE-DTLSLVTADH-----------SHVFSFGGYPLRGSSIFGLA 397
Query: 327 KEIKSGGDIRD-------VIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKL 379
G RD + G L D ++ E+++ + +Y ++
Sbjct: 398 P-----GKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEY----------RQQS 442
Query: 380 GVGFVSHKHTGEPVPLLAYGPGAENFVGFKHHVDTAKVIA 419
V H GE V + A GP A G + A V+A
Sbjct: 443 AVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMA 482
>gi|11428784 alkaline phosphatase, intestinal [Homo sapiens]
Length = 476
Score = 127 bits (317), Expect = 2e-28
Identities = 98/326 (30%), Positives = 155/326 (47%), Gaps = 62/326 (19%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVTDSA 80
+N+I+ +GDG+G + T+++ G N M+ FPY + T ++ +V DSA
Sbjct: 53 KNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSA 112
Query: 81 AAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTRITH 133
A TA GVK + I + T + + +++ A+ GK+ G+VTTTR+ H
Sbjct: 113 ATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQH 172
Query: 134 ATPAVFASHVPDR------DME--------EEIARQLILH-NVTVLMGGGREKF------ 172
A+PA +H +R DM ++IA QLI + ++ V++GGGR+
Sbjct: 173 ASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTP 232
Query: 173 --------SEEVLKL----------AEDYGYSIVYTREDLEKVKDGK----VLGLFAEGH 210
S+ ++L A+ G V+ R +L + + ++GLF G
Sbjct: 233 DPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGD 292
Query: 211 LPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETKE 269
Y + R D SL+EMT+ A+ +L +NP GF+L +EGGRIDH H + E
Sbjct: 293 TKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVM 352
Query: 270 FDDVVGYVLDYARRRGDTLVIVLADH 295
FDD + DTL +V ADH
Sbjct: 353 FDDAIERAGQLTSEE-DTLTLVTADH 377
>gb|AAF46819.1| (AE003456) CG3292 gene product [Drosophila melanogaster]
Length = 543
Score = 127 bits (316), Expect = 3e-28
Identities = 102/329 (31%), Positives = 151/329 (45%), Gaps = 65/329 (19%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGHL----------NMEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGM S + ++ G L + E FPYTG+ T + +V DSA
Sbjct: 94 KNVILFLGDGMSLSTVAAARIHKGQLKGNTGEEDSLSFEKFPYTGLSRTYCSNAQVPDSA 153
Query: 81 AAGTAIATGVKT------YNRMISTTNVTGK---LVNLTTLLEIAQMLGKATGLVTTTRI 131
TA GVKT +S N +G + ++ AQ GKATG+VTTT +
Sbjct: 154 CTATAYLCGVKTNIVALGITAAVSFNNCSGSEDPANQVDSIAAWAQAAGKATGIVTTTTL 213
Query: 132 THATPAVFASHVPDRDMEEE------------------IARQLILH----NVTVLMGGGR 169
THA+P+ + +R E + IA QLI N V++GGG
Sbjct: 214 THASPSGAYAKTTNRFFESDTDILTYGEGQNDPATCTDIATQLITQAPGKNFDVMLGGGI 273
Query: 170 EKF---------------SEEVLKLAEDYGYS----IVYTREDLEKVKDGKV---LGLFA 207
KF S+ V L+ G + Y R L V K+ +G F
Sbjct: 274 GKFLPNTITDPFNKKGERSDGVNLLSRWQGLHPGGVLAYNRNQLLSVNVSKITNLIGTFR 333
Query: 208 EGHLPY-VLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
G + + + +E+ +L EMT+KAIE++ K G+FL +EGG ID+ H N ++E
Sbjct: 334 SGVMSFNKMADPKEEPTLAEMTRKAIEVVSKRDGGYFLFVEGGLIDYGNHFNSPTYSLSE 393
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADH 295
T +F+ V LD +TL++V +DH
Sbjct: 394 TLQFEQAVQEALDITDPE-ETLIVVTSDH 421
>sp|P29523|PPB_BOMMO MEMBRANE-BOUND ALKALINE PHOSPHATASE PRECURSOR (M-ALP)
>gi|102886|pir||S19607 alkaline phosphatase (EC 3.1.3.1)
- silkworm >gi|217282|dbj|BAA14420.1| (D90454)
membrane-bound alkaline phosphatase (m-ALP) [Bombyx
mori]
Length = 547
Score = 126 bits (314), Expect = 5e-28
Identities = 105/338 (31%), Positives = 152/338 (44%), Gaps = 66/338 (19%)
Query: 23 ANASPSGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSL 72
A + +NV++ +GDGM L + + G L+ E FP G+ T +
Sbjct: 64 AKQAAGHAKNVVMFLGDGMSVPTLAAARTLLGQRRGQTGEEASLHFEQFPTLGLAKTYCV 123
Query: 73 SGEVTDSAAAGTAIATGVKTYNRMISTTNV---------TGKLVNLTTLLEIAQMLGKAT 123
+ +V DS+ TA GVK T T + ++ E A G+
Sbjct: 124 NAQVPDSSCTATAYLCGVKANQGTPGVTAAVPRHDCEASTDVTKRVQSIAEWALADGRDV 183
Query: 124 GLVTTTRITHATPAVFASHVPDRDMEEE---------------IARQLIL----HNVTVL 164
G+VTTTRITHA+PA + V +R+ E + IA QLI + V+
Sbjct: 184 GIVTTTRITHASPAGTFAKVANRNWENDNDVKQEGHDVNRCPDIAHQLIKMAPGNKFKVI 243
Query: 165 MGGGREKF------SEEVLK--------LAEDY---------GYSIVYTREDLEKVKDGK 201
GGGR +F EE + L E++ Y ++ R++L K+
Sbjct: 244 FGGGRREFLPTTQVDEEGTRGLRTDGRNLIEEWQNDKESQKVSYKYLWNRQELLKLGSSP 303
Query: 202 ---VLGLFAEGHLPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHA 257
+LGLF HL Y L+ E + +L E+T AI +L +N GFFL +EGGRIDHA H
Sbjct: 304 PDYLLGLFEGSHLQYHLEGDESTEPTLAELTDVAIRVLSRNERGFFLFVEGGRIDHAHHD 363
Query: 258 NDVASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
N + ET E D V D A + ++LV+V ADH
Sbjct: 364 NYAHLALDETIEMDRAVKVATD-ALKEDESLVVVTADH 400
>gb|AAC33854.1| (AF052227) intestinal alkaline phosphatase IV; IAP IV [Bos taurus]
Length = 530
Score = 125 bits (311), Expect = 1e-27
Identities = 96/329 (29%), Positives = 154/329 (46%), Gaps = 62/329 (18%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVT 77
+ +NVI+ +GDGMG + T+++ G +N M+ FPY + T ++ +V
Sbjct: 50 TAAKNVILFLGDGMGVPTVTATRILKGQMNGKLGPETPLAMDQFPYVALSKTYNVDRQVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DSA TA GVK + I + T +T+++ A+ GK+ G+VTT+R
Sbjct: 110 DSAGTATAYLCGVKGNYKTIGVSAAARYNQCNTTSGNEVTSVMNRAKKAGKSVGVVTTSR 169
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGREKFSEE 175
+ HA+PA +H +R+ ++IA QL+ + ++ V++GGGR E
Sbjct: 170 VQHASPAGAYAHTVNRNWYSDADLPADAQTYGCQDIATQLVNNMDIDVILGGGRMYMFPE 229
Query: 176 ---------------VLK---------LAEDYGYSIVYTR-EDLEKVKDGKV---LGLFA 207
V K A+ G V+ R E L+ D V +GLF
Sbjct: 230 GTPDPEYPYDVNQTGVRKDKRNLVQEWQAKHQGAQYVWNRTELLQAANDPSVTHLMGLFE 289
Query: 208 EGHLPY-VLDRSEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
+ Y V +D +L EMT+ A+++L +NP GF+L +EGGRIDH H + +
Sbjct: 290 PADMKYNVQQDPTKDPTLEEMTEAALQVLSRNPQGFYLFVEGGRIDHGHHEGKAYMALTD 349
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADH 295
T FD+ + + DTL++ ADH
Sbjct: 350 TVMFDNAIAKANELTSEL-DTLILATADH 377
>gi|6671535 alkaline phosphatase 3, intestine, not Mn requiring [Mus musculus]
>gi|130745|sp|P24822|PPBI_MOUSE ALKALINE PHOSPHATASE,
INTESTINAL PRECURSOR (IAP) >gi|109535|pir||B36307
alkaline phosphatase (EC 3.1.3.1), intestinal - mouse
>gi|194049|gb|AAA37873.1| (M61705) intestinal alkaline
phosphatase [Mus musculus]
Length = 559
Score = 121 bits (300), Expect = 2e-26
Identities = 100/329 (30%), Positives = 155/329 (46%), Gaps = 62/329 (18%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG + T+++ G L M+ FPY + T S+ +V
Sbjct: 50 TSAKNLIIFLGDGMGVPTVTATRILKGQLEGHLGPETPLAMDRFPYMALSKTYSVDRQVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTTNVTG-KLVNLT------TLLEIAQMLGKATGLVTTTR 130
DSA+ TA GVKT + I + N T +++ A+ GK+ G+VTTTR
Sbjct: 110 DSASTATAYLCGVKTNYKTIGLSAAARFDQCNTTFGNEVFSVMYRAKKAGKSVGVVTTTR 169
Query: 131 ITHATPAVFASHVPDR------DME--------EEIARQLILH-NVTVLMGGGRE----- 170
+ HA+P+ H +R DM ++IA QLI + ++ V++GGGR+
Sbjct: 170 VQHASPSGTYVHTVNRNWYGDADMPASALREGCKDIATQLISNMDINVILGGGRKYMFPA 229
Query: 171 -----KFSEEVLK--------------LAEDYGYSIVYTREDL-EKVKDGKV---LGLFA 207
++ + + L++ G V+ RE L +K +D V +GLF
Sbjct: 230 GTPDPEYPNDANETGTRLDGRNLVQEWLSKHQGSQYVWNREQLIQKAQDPSVTYLMGLFE 289
Query: 208 EGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAE 266
+ + R D SL +MT+ A+++L +NP GF+L +EGGRID H + E
Sbjct: 290 PVDTKFDIQRDPLMDPSLKDMTETAVKVLSRNPKGFYLFVEGGRIDRGHHLGTAYLALTE 349
Query: 267 TKEFDDVVGYVLDYARRRGDTLVIVLADH 295
FD + R DTL IV ADH
Sbjct: 350 AVMFDLAIERASQLTSER-DTLTIVTADH 377
>gb|AAF36718.1|AF227508_1 (AF227508) intestinal alkaline phosphatase-II [Rattus norvegicus]
Length = 562
Score = 120 bits (297), Expect = 5e-26
Identities = 100/330 (30%), Positives = 154/330 (46%), Gaps = 64/330 (19%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG + T+++ G L M+ FPY + T S+ +V
Sbjct: 50 TSAKNLIIFLGDGMGVPTVTATRILKGQLEGNLGPETPLAMDHFPYMALSKTYSVDRQVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTT--------NVTGKLVNLTTLLEIAQMLGKATGLVTTT 129
DSA+ TA GVKT + I + N T L+ + A+ GK+ G+VTTT
Sbjct: 110 DSASTATAYLCGVKTNYKTIGVSAAARFDQCNTTFGNEVLSVMYR-AKKAGKSVGVVTTT 168
Query: 130 RITHATPAVFASHVPDR------DME--------EEIARQLILH-NVTVLMGGGRE---- 170
R+ HA+PA H +R DM ++IA QLI + ++ V++GGGR+
Sbjct: 169 RVQHASPAGTYVHTVNRNWYGDADMPASALQEGCKDIATQLISNMDINVILGGGRKYMFP 228
Query: 171 ------KFSEEVLK--------------LAEDYGYSIVYTREDL-EKVKDGKV---LGLF 206
++ +V + L++ G V+ R++L +K D V +GLF
Sbjct: 229 AGTPDPEYPNDVNETGTRLDGKNLVQEWLSKHQGSQYVWNRQELIQKSLDPSVTYLMGLF 288
Query: 207 AEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVA 265
+ + R D SL +MT+ A+ +L +NP GF+L +EGGRID H +
Sbjct: 289 EPVDTKFEIQRDPLMDPSLKDMTEAALHVLSRNPKGFYLFVEGGRIDRGHHLGTAYLALT 348
Query: 266 ETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
E D + + DTL IV ADH
Sbjct: 349 EAVMLDSAIERASQLTSEQ-DTLTIVTADH 377
>gb|AAF46820.1| (AE003456) CG3290 gene product [Drosophila melanogaster]
Length = 538
Score = 118 bits (294), Expect = 1e-25
Identities = 112/438 (25%), Positives = 181/438 (40%), Gaps = 80/438 (18%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVTDSA 80
+NVI+ +GDGM S + +++ G L+ E FPY+G+ T + +V DSA
Sbjct: 89 KNVIMFLGDGMPISTVTAARILKGQRQGNTGEESSLSFEKFPYSGLSRTYCANAQVPDSA 148
Query: 81 AAGTAIATGVKT------YNRMISTTNVTGK---LVNLTTLLEIAQMLGKATGLVTTTRI 131
TA GVKT + ++ N T LT++ E AQ GK+TG VTTT +
Sbjct: 149 CTATAYLCGVKTNIINIGVSAAVNFNNCTASQDPANRLTSIAEWAQNAGKSTGFVTTTTL 208
Query: 132 THATPAVFASHVPDR-------------------DMEEEIARQLILHNVTVLMGGGREKF 172
THA+P+ + +R DM ++ Q N V+ GGG KF
Sbjct: 209 THASPSGAYAKTANRMWQSDTDVTSYGVDASTCVDMATQLVTQTPGKNFEVMFGGGMGKF 268
Query: 173 SEEVLKLAE--------------------DYGYSIVYTRE--DLEKVKDGKVLGLFAEGH 210
+ K + D G + ++ +L+ ++G F G
Sbjct: 269 LPKTTKDSHGKYGERSDGVNLLSRWQGLHDKGVLVTNRKQLLNLDVSAASSIIGTFQSGL 328
Query: 211 LPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAETKE 269
+ + +D +L E+T+ AI+ L N NG+F+ IEGG ID+ H + E E
Sbjct: 329 MDFHMDADPTYQPTLSELTEVAIKKLSHNENGYFVFIEGGLIDYGNHYTQAGYALDEALE 388
Query: 270 FDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEMSKEI 329
F+ + + DTL++V ADH + I G I + + D I + +
Sbjct: 389 FEKAIQLAREMT-NISDTLIVVTADH-GHAVSIAGYPGRGTPILGLNQHDTDINGVKYSV 446
Query: 330 KSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVSHKHT 389
+ + G + +++ ++A I I K++GV H+
Sbjct: 447 LNYAAGPNQYLDEHGQRIPLDDILGPDDA-----------ISPSYIDKEIGV------HS 489
Query: 390 GEPVPLLAYGPGAENFVG 407
GE V + A GP + F G
Sbjct: 490 GEDVGIWASGPQSHLFTG 507
>sp|P51740|PPBJ_RAT ALKALINE PHOSPHATASE, INTESTINAL 2 PRECURSOR (IAP-II)
>gi|111353|pir||S18408 alkaline phosphatase (EC 3.1.3.1)
- rat >gi|8178944|gb|AAB20378.2| (S66545) putative
alkaline phosphatase [Rattus sp.]
Length = 551
Score = 115 bits (286), Expect = 9e-25
Identities = 99/330 (30%), Positives = 155/330 (46%), Gaps = 64/330 (19%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGHLN----------MEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG + + T+++ G L M+ FPY + T S+ +V
Sbjct: 50 TSAKNLIIFLGDGMGVATVTATRILKGQLEGNLGPETPLAMDHFPYMALSKTYSVDRQVP 109
Query: 78 DSAAAGTAIATGVKTYNRMISTT--------NVTGKLVNLTTLLEIAQMLGKATGLVTTT 129
DSA+ TA GVKT + I + N T L+ + A+ GK+ G+ T
Sbjct: 110 DSASTATAYLCGVKTNYKTIGVSAAARFDQCNTTFGNEVLSVMYR-AKKAGKSVGVGDHT 168
Query: 130 RITHATPA------VFASHVPDRDME--------EEIARQLILH-NVTVLMGGGRE---- 170
R+ HA+PA V ++ D DM ++IA QLI + ++ V++GGGR+
Sbjct: 169 RVQHASPAGTYVHTVTSNWYGDADMPALPLQEGCKDIATQLISNMDINVILGGGRKYMFP 228
Query: 171 ------KFSEEVLK--------------LAEDYGYSIVYTREDL-EKVKDGKV---LGLF 206
++ +V + L++ G V+ R++L +K D V +GLF
Sbjct: 229 AGTPDPEYPNDVNETGTRLDGKNLVQEWLSKHQGSQYVWNRQELIQKSLDPSVTYLMGLF 288
Query: 207 AEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVA 265
+ + R D SL +MT+ A+ +L +NP GF+L +EGGRID H +
Sbjct: 289 EPVDTKFEIQRDPLMDPSLKDMTEAALHVLSRNPKGFYLFVEGGRIDRGHHLGTAYLALT 348
Query: 266 ETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
E FD + A + DTL IV ADH
Sbjct: 349 EAVMFDSAIERASLQASEQ-DTLTIVTADH 377
>dbj|BAA34926.1| (AB013386) soluble alkaline phosphatase [Bombyx mori]
Length = 450
Score = 114 bits (283), Expect = 2e-24
Identities = 94/328 (28%), Positives = 146/328 (43%), Gaps = 71/328 (21%)
Query: 35 ILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVTDSAAAGT 84
+ +GDGM + L + + G L+ E FP G+ T L +V DSA + +
Sbjct: 1 MFLGDGMSVATLTAARTLLGQRRGQTGEESRLSFEHFPTVGLSKTYCLDAQVADSACSAS 60
Query: 85 AIATGVKTYNRMISTTNVTGKLV--NLTTLLEIAQMLG----------KATGLVTTTRIT 132
A G K + T V+G + + T + A L + G+VTTTR+T
Sbjct: 61 AYLCGAKAN---LGTIGVSGHVARHHCTAATDAAHQLASIASWALDADRDAGIVTTTRVT 117
Query: 133 HATPAVFASHVPDRDMEEE--------------IARQLIL----HNVTVLMGGGREKFSE 174
HA+PA +H DR+ E + IA+QL+ + V++GGGR +F
Sbjct: 118 HASPAGAYAHTADRNWESDGDVTAGCSGHAQLDIAQQLVHARPGKDFKVILGGGRREFLP 177
Query: 175 EVL-----------------------KLAEDYGYSIVYTREDLEKVKDGK---VLGLFAE 208
+ K + + V+ R+ L +V + +LGLF
Sbjct: 178 NTMTDEEGSRGRRYDGRNLISEWLTEKDSRGVTHEYVWNRQQLMEVSEELPEYLLGLFEP 237
Query: 209 GHLPYVLDRSEE-DVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDVASIVAET 267
HL Y ++ + + +L E+T+ AI+ L +N GFFL +EGGRIDHA H N + ET
Sbjct: 238 SHLQYHMEANHTTEPTLAELTEVAIKSLSRNKEGFFLFVEGGRIDHAHHENLAHLALDET 297
Query: 268 KEFDDVVGYVLDYARRRGDTLVIVLADH 295
E V + + D+L++V ADH
Sbjct: 298 IELSAAVEKARELLSEQ-DSLIVVTADH 324
>gb|AAF46821.1| (AE003456) CG3264 gene product [Drosophila melanogaster]
Length = 533
Score = 111 bits (276), Expect = 1e-23
Identities = 117/442 (26%), Positives = 182/442 (40%), Gaps = 87/442 (19%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVTDSA 80
+N+I+ +GDGM S + +++ G L+ E FPYTG+ T + +V DSA
Sbjct: 88 KNIIMFLGDGMPLSTVAAARILKGQRQGNTGEESSLSFERFPYTGLSRTYCSNAQVPDSA 147
Query: 81 AAGTAIATGVKT------------YNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTT 128
TA GVKT YN ++ + +L T++ E AQ GK+TG+VTT
Sbjct: 148 CTATAYLCGVKTNIVNIGVSAAVEYNNCTASQDPANRL---TSIAEWAQNAGKSTGIVTT 204
Query: 129 TRITHATPAVFASHVPDR-------------------DMEEEIARQLILHNVTVLMGGGR 169
T +THA+P+ + +R DM ++ Q N V+ GGG
Sbjct: 205 TTLTHASPSGAYAKTANRMWECDTDVTSYGVDANSCIDMATQLVTQTPGKNFEVMFGGGM 264
Query: 170 EKF---------------SEEVLKLAEDYGYS----IVYTREDLEKVK---DGKVLGLFA 207
KF S+ V L+ G +V R L K+ V+GLF
Sbjct: 265 GKFLPNSIVDSHGNPGERSDGVNLLSRWQGLHDGGVLVTNRNQLLKLNVSAVSSVIGLFQ 324
Query: 208 EGHLPYVLDRSEE-DVSLLEMTKKAIEILEKN-PNGFFLMIEGGRIDHACHANDVASIVA 265
+ + L+ + +L E+T+ I+ L +N +G+F IEGG ID+ H +
Sbjct: 325 SKLMNFHLEADDTYQPTLSELTEVTIKKLSQNKEHGYFAFIEGGLIDYGNHYTKAGYALD 384
Query: 266 ETKEFDDVVGYVLDYARRRGDTLVIVLADHETGGLGIGLNYGHSVDIDSIRRIDASIEEM 325
E EF+ + D DTL++V ADH + I G I I + D I +
Sbjct: 385 EALEFEKAIQLARDMTDIE-DTLIVVTADH-GHAVSIAGYPGRGTPILGINQHDTDINGV 442
Query: 326 SKEIKSGGDIRDVIRRHTGLELTDEEVKEIEEAKNSTNKYALGNIIGEIISKKLGVGFVS 385
+ + + G + +++ ++A I I K GV
Sbjct: 443 QYSVLNYAAGPNQYLDENGQRIPLDDILGSDDA-----------ITPSYIPKDQGV---- 487
Query: 386 HKHTGEPVPLLAYGPGAENFVG 407
H+GE V + A GP + F G
Sbjct: 488 --HSGEDVGIFASGPQSHLFTG 507
>emb|CAC18282.1| (AL451020) alkaline phosphatase [Neurospora crassa]
Length = 668
Score = 104 bits (258), Expect = 2e-21
Identities = 99/338 (29%), Positives = 146/338 (42%), Gaps = 70/338 (20%)
Query: 25 ASPSGVRNVIILIGDGMGFSQLQLTKLV---------YGHLNMEDFPYTGIELTDSLSGE 75
A+ +NVI+ IGDGM + + +L+ L ++ FP G ++T S+
Sbjct: 163 ATKKKAKNVILFIGDGMTTNMITAARLLAHKSINGKYQSTLQLDKFPTLGHQMTHSIDSF 222
Query: 76 VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVN----------LTTLLEIAQML-GKATG 124
+TDSA + +A+ TG KT T N G V+ + T++E+ + + G A G
Sbjct: 223 ITDSANSASALYTGHKT------TVNAMGVYVDSSPDKFDDPKVETIVELLKRIWGSAWG 276
Query: 125 LVTTTRITHATPAVFASHVPDRDMEEEIARQLI--LHNVT--------VLMGGGREKF-S 173
V+T I ATP +H R + Q + N + V GGG E+F
Sbjct: 277 AVSTAFIADATPTALTAHTRSRYEYGALIEQALNGFSNTSWTKQDGPDVYFGGGAEQFLP 336
Query: 174 EEVLKLAEDY-------GYSIVYTREDLEKVKDG-KVLGLFAEGHLPYVLDRS---EEDV 222
+DY GYS+ + L + D K LG+F + +LP LDR+ E +
Sbjct: 337 SSASYQGKDYYAEFAKKGYSVSLNKTSLLALPDDKKALGIFCKSNLPVWLDRNIFPENTM 396
Query: 223 SL-----------------LEMTKKAIEILEK--NPNGFFLMIEGGRIDHACHANDVASI 263
L EMT KAI+IL K +GFFLM E ID H D
Sbjct: 397 KLKNDPSGASGPAKDIPGQKEMTLKAIDILHKRGGKDGFFLMSEAASIDKQMHVLDYERA 456
Query: 264 VAETKEFDDVVGYVLDYARRRGD---TLVIVLADHETG 298
+ + E DD V + + G+ TL++V ADH G
Sbjct: 457 LGDLLELDDTVRATIQKLQDLGELNNTLIVVTADHGHG 494
>pir||T47236 alkaline phosphatase (EC 3.1.3.1) (pho-2+) [imported] - Neurospora
crassa >gi|450864|gb|AAA21821.1| (L27993) alkaline
phosphatase [Neurospora crassa]
Length = 555
Score = 103 bits (255), Expect = 4e-21
Identities = 98/338 (28%), Positives = 144/338 (41%), Gaps = 70/338 (20%)
Query: 25 ASPSGVRNVIILIGDGMGFSQLQLTKLV---------YGHLNMEDFPYTGIELTDSLSGE 75
A+ +NVI+ IGDGM + + +L+ L ++ FP G ++T S+
Sbjct: 163 ATKKKAKNVILFIGDGMTTNMITAARLLAHKSINGKYQSTLQLDKFPTLGHQMTHSIDSF 222
Query: 76 VTDSAAAGTAIATGVKTYNRMISTTNVTGKLVN----------LTTLLEIAQML-GKATG 124
+TDSA + +A+ TG KT T N G V+ + T++E+ + + G A G
Sbjct: 223 ITDSANSASALYTGHKT------TVNAMGVYVDSSPDKFDDPKVETIVELLKRIWGSAWG 276
Query: 125 LVTTTRITHATPAVFASHVPDRDMEEEIARQLI----------LHNVTVLMGGGREKF-S 173
V+T I ATP +H R + Q + V GGG E+F
Sbjct: 277 AVSTAFIADATPTALTAHTRSRYEYGALIEQALNGFSKPSWTKQDGPDVYFGGGAEQFLP 336
Query: 174 EEVLKLAEDY-------GYSIVYTREDLEKVKDG-KVLGLFAEGHLPYVLDRS---EEDV 222
+DY GYS+ + L + D K LG+F + +LP LDR+ E +
Sbjct: 337 SSASYQGKDYYAEFAKKGYSVSLNKTSLLALPDDKKALGIFCKSNLPVWLDRNIFPENTM 396
Query: 223 SL-----------------LEMTKKAIEILEK--NPNGFFLMIEGGRIDHACHANDVASI 263
L EMT KAI+IL K +GFFLM E ID H D
Sbjct: 397 KLKNDPSGASGPAKDIPGQKEMTLKAIDILHKRGGKDGFFLMSEAASIDKQMHVLDYERP 456
Query: 264 VAETKEFDDVVGYVLDYARRRGD---TLVIVLADHETG 298
+ + E DD V + + G+ TL++V ADH G
Sbjct: 457 LGDLLELDDTVRATIQKLQDLGELNNTLIVVTADHGHG 494
>gb|AAF25669.1| (AF209193) alkaline phosphatase [Schizophyllum commune]
>gi|6707495|gb|AAF25670.1| (AF209194) alkaline
phosphatase [Schizophyllum commune]
Length = 604
Score = 102 bits (253), Expect = 7e-21
Identities = 102/376 (27%), Positives = 158/376 (41%), Gaps = 77/376 (20%)
Query: 31 RNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVTDSA 80
+NV++ IGDGM S + +L+ GH + M+ G ++T SL +TDSA
Sbjct: 166 KNVLLFIGDGMTQSMITAARLI-GHKSINGRYQSLMQMDQMDALGHQMTHSLDSFITDSA 224
Query: 81 AAGTAIATGVKT-YNRMISTTNVTGKLVNLTTLLEIAQMLGKAT----GLVTTTRITHAT 135
+ TA+ TG K+ N + + + + IA+M + T G+V+T I AT
Sbjct: 225 NSATALYTGRKSSVNALNVYADSSATPFDDPKHETIAEMFRRETNGGLGIVSTAVIGDAT 284
Query: 136 PAVFASHVPDRD---------------MEEEIARQLILHNVTVLMGGGREKF--SEEVLK 178
PA +H DRD + +A + V+ GGG E F E+ +
Sbjct: 285 PAALCAHTRDRDQLAAVVYEYLYSPEELNSTLAWPTSCKSPDVIFGGGAELFLAEEDAFE 344
Query: 179 LAEDYG------YSIVYTREDLEKV-KDGKVLGLFAEGHLPYVLDR-------------- 217
+ YG Y++V+ + L D K LG+F+ ++ LDR
Sbjct: 345 GRDMYGEFGARGYNVVFNKTALASAPNDTKTLGIFSTSNMAKWLDRLVYPDNLKDKANSP 404
Query: 218 ------SEEDVSLLEMTKKAIEIL--EKNPNGFFLMIEGGRIDHACHANDVASIVAETKE 269
+ + L +MT KAI+IL + G+F+M E ID H D + E E
Sbjct: 405 TGDGSDATDQPGLKDMTLKAIDILNAQYKEEGWFMMSEAASIDKMMHVLDYDRALGELLE 464
Query: 270 FDDVVGYVLDYARRRGD---TLVIVLADHETGGLGIGLNYGHSVDI-DSIRRIDASIEEM 325
DD + L Y + G+ TLV+V ADH GH D+ + S +E
Sbjct: 465 LDDTIRATLAYLKAIGEDENTLVVVTADH-----------GHGFDVFGGADTMYVSAQED 513
Query: 326 SKEIKSGGDIRDVIRR 341
+ + GG R + R
Sbjct: 514 DRSKRRGGAFRSSLSR 529
>gb|AAD45540.1|U70376_2 (U70376) streptomycin-6-phosphate phosphatase [Streptomyces
flavopersicus]
Length = 256
Score = 96.3 bits (236), Expect = 7e-19
Identities = 62/129 (48%), Positives = 75/129 (58%), Gaps = 11/129 (8%)
Query: 27 PSGVRNVIILIGDGMGFSQLQLTK----LVYGHLNMEDFPYTGIELTDSLSGE-----VT 77
PS RNVI+LIGDGMG S++ + G L M+ P TG LT ++ VT
Sbjct: 56 PSPARNVILLIGDGMGDSEITAARNYAVAAEGRLTMDRLPMTGSSLTSAVDERGHPDYVT 115
Query: 78 DSAAAGTAIATGVKTYNRMISTTNVTGKLVNLTTLLEIAQMLGKATGLVTTTRITHATPA 137
DSAA TA ATG KT N IS T T + L TLLE+A+ G ATG VTT ++T ATPA
Sbjct: 116 DSAAGATAWATGRKTVNGRISRTPDTNE--PLPTLLELARREGYATGNVTTAQLTDATPA 173
Query: 138 VFASHVPDR 146
+HV DR
Sbjct: 174 ALTAHVTDR 182
>dbj|BAA88367.1| (AB012643) alkaline phosphatase [Homo sapiens]
Length = 341
Score = 77.3 bits (187), Expect = 4e-13
Identities = 43/95 (45%), Positives = 53/95 (55%), Gaps = 2/95 (2%)
Query: 202 VLGLFAEGHLPYVLDRSE-EDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDV 260
+LGLF G + Y L+R+ D SL EM AI+IL KNP GFFL++EGGRIDH H
Sbjct: 103 LLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKA 162
Query: 261 ASIVAETKEFDDVVGYVLDYARRRGDTLVIVLADH 295
+ E E D +G DTL +V ADH
Sbjct: 163 KQALHEAVEMDRAIGQAGSLTSSE-DTLTVVTADH 196
>gi|11428788 alkaline phosphatase, placental (Regan isozyme) [Homo sapiens]
Length = 471
Score = 73.7 bits (178), Expect = 4e-12
Identities = 52/175 (29%), Positives = 87/175 (49%), Gaps = 32/175 (18%)
Query: 28 SGVRNVIILIGDGMGFSQLQLTKLVYGH----------LNMEDFPYTGIELTDSLSGEVT 77
+ +N+II +GDGMG S + +++ G L M+ FPY + T ++ V
Sbjct: 53 TAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVP 112
Query: 78 DSAAAGTAIATGVKTYNRMISTTNV-------TGKLVNLTTLLEIAQMLGKATGLVTTTR 130
DS A TA GVK + I + T + + +++ A+ GK+ G+VTTTR
Sbjct: 113 DSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTR 172
Query: 131 ITHATPAVFASHVPDRDME--------------EEIARQLILH-NVTVLMGGGRE 170
+ HA+PA +H +R+ ++IA QLI + ++ V++GGGR+
Sbjct: 173 VQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK 227
Score = 70.6 bits (170), Expect = 4e-11
Identities = 36/84 (42%), Positives = 49/84 (57%), Gaps = 1/84 (1%)
Query: 202 VLGLFAEGHLPYVLDR-SEEDVSLLEMTKKAIEILEKNPNGFFLMIEGGRIDHACHANDV 260
++GLF G + Y + R S D SL+EMT+ A+ +L +NP GFFL +EGGRIDH H +
Sbjct: 386 LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA 445
Query: 261 ASIVAETKEFDDVVGYVLDYARRR 284
+ ET FDD + RR
Sbjct: 446 YRALTETIMFDDAIERAASSPARR 469
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.318 0.138 0.382
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 177034070
Number of Sequences: 2977
Number of extensions: 7683324
Number of successful extensions: 22720
Number of sequences better than 1.0e-10: 118
Number of HSP's better than 0.0 without gapping: 46
Number of HSP's successfully gapped in prelim test: 72
Number of HSP's that attempted gapping in prelim test: 22124
Number of HSP's gapped (non-prelim): 223
length of query: 495
length of database: 189,106,746
effective HSP length: 60
effective length of query: 435
effective length of database: 153,197,526
effective search space: 66640923810
effective search space used: 66640923810
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 167 (69.5 bits)