BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2402 (aIF-2BII) DE:translation initiation factor aIF-2BII
translation initiation factor
(324 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9V281|E2B2_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 649 0.0
sp|O57947|E2B2_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 605 e-172
sp|Q57586|E2B2_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 318 5e-86
gi|11499619 translation initiation factor eIF-2B, subunit delta ... 310 2e-83
gb|AAG20059.1| (AE005085) translation initiation factor eIF-2B s... 290 1e-77
sp|Q57896|E2B1_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 171 1e-41
gi|11497982 translation initiation factor eIF-2B, subunit delta ... 169 5e-41
sp|Q9YE84|E2B1_AERPE PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 165 5e-40
sp|P74497|E2B_SYNY3 PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 160 2e-38
sp|Q9X013|E2B_THEMA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 158 9e-38
sp|Q9PAG5|E2B_XYLFA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 156 3e-37
sp|O58185|E2BL_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 154 1e-36
sp|O67879|E2B_AQUAE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 153 2e-36
sp|O58433|E2B1_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 152 7e-36
sp|Q9UYB6|E2BL_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 147 1e-34
sp|Q9UZ16|E2B1_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 147 1e-34
gb|AAK43082.1| Translation initiation factor aiF-2B, subunit 1 (... 146 2e-34
sp|O27900|E2B1_METTH PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 145 5e-34
emb|CAC12337.1| (AL445066) translation initiation factor aIF-2B,... 145 7e-34
pir||A83249 probable initiation factor 2 subunit PA3169 [importe... 142 6e-33
sp|O31662|E2B_BACSU PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 139 4e-32
sp|Q63186|E2BD_RAT TRANSLATION INITIATION FACTOR EIF-2B DELTA SU... 138 1e-31
emb|CAA53204.1| (X75451) translation initiation factor eIF-2B-de... 137 2e-31
sp|P41111|E2BD_RABIT TRANSLATION INITIATION FACTOR EIF-2B DELTA ... 137 2e-31
pir||T08757 probable translation initiation factor eIF-2B delta ... 137 2e-31
emb|CAB57260.1| (AJ011305) guanine nucleotide exchange factor, d... 137 2e-31
emb|CAB57261.1| (AJ011306) guanine nucleotide exchange factor (l... 137 2e-31
emb|CAB57305.1| (AJ011307) guanine nucleotide exchange factor [H... 137 2e-31
sp|Q9UI10|E2BD_HUMAN TRANSLATION INITIATION FACTOR EIF-2B DELTA ... 137 2e-31
pir||A55146 guanine nucleotide exchange factor eIF-2B delta chai... 135 8e-31
gi|6753736 eukaryotic translation initiation factor 2B [Mus musc... 134 2e-30
gb|AAA68046.1| (M98035) guanine nucleotide exchange factor, eIF-... 134 2e-30
dbj|BAB10154.1| (AB005231) contains similarity to guanine nucleo... 133 2e-30
gb|AAF27156.1|AC016529_19 (AC016529) putative translation initia... 127 1e-28
gb|AAF02860.1|AC009324_9 (AC009324) Similar to initiation factor... 127 2e-28
sp|Q09924|E2BD_SCHPO PROBABLE TRANSLATION INITIATION FACTOR EIF-... 127 2e-28
pir||T00687 hypothetical protein F6E13.20 - Arabidopsis thaliana... 127 2e-28
gb|AAC95160.1| (AC005970) putative translation initiation factor... 122 6e-27
gb|AAF57154.1| (AE003778) CG11334 gene product [alt 1] [Drosophi... 120 2e-26
gb|AAF46995.1| (AE003460) CG10315 gene product [Drosophila melan... 118 6e-26
emb|CAB90925.1| (AL355774) putative translation initiation facto... 117 2e-25
pir||T40858 translation initiation factor eif-2b alpha subunit -... 114 1e-24
gb|AAF69705.1|AC016041_10 (AC016041) F27J15.24 [Arabidopsis thal... 113 4e-24
sp|P34604|E2BA_CAEEL PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 112 5e-24
gi|6322878 34 KD alpha subunit of eIF2B; Gcn3p [Saccharomyces ce... 111 1e-23
sp|Q93169|YQJQ_CAEEL HYPOTHETICAL 40.9 KD PROTEIN C01G10.9 IN CH... 108 7e-23
gb|AAF20216.1|AC012395_3 (AC012395) putative translation initiat... 108 1e-22
pir||T39944 hypothetical translation initiation factor - fission... 107 2e-22
gb|AAD52847.2|AF137288_1 (AF137288) putative translation initiat... 106 5e-22
gi|4503503 eukaryotic translation initiation factor 2B, subunit ... 104 2e-21
sp|Q64270|E2BA_RAT TRANSLATION INITIATION FACTOR EIF-2B ALPHA SU... 101 9e-21
gb|AAG19758.1| (AE005061) translation initiation factor eIF-2B s... 97 2e-19
gi|6325375 Ypr118wp [Saccharomyces cerevisiae] >gi|3183489|sp|Q0... 97 3e-19
gb|AAF56977.1| (AE003771) CG7883 gene product [Drosophila melano... 96 7e-19
gb|AAG38014.1| (AF289100) eukaryotic initiation factor eIF2B alp... 96 7e-19
gi|6321520 translation initiation factor eIF2B, 71 kDa (delta) s... 93 4e-18
gb|AAF02141.1|AC009853_1 (AC009853) putative translation initiat... 92 6e-18
sp|Q90511|E2BB_FUGRU TRANSLATION INITIATION FACTOR EIF-2B BETA S... 92 8e-18
gb|AAF61750.1|AF064872_1 (AF064872) translation initiation facto... 86 6e-16
gi|7657058 eukaryotic translation initiation factor 2B, subunit ... 85 8e-16
emb|CAA19648.1| (AL023874) /match=(desc:""TRANSLATION INITIATION... 85 8e-16
sp|Q62818|E2BB_RAT TRANSLATION INITIATION FACTOR EIF-2B BETA SUB... 82 1e-14
gb|AAB58527.1| (U83914) eukaryotic initiation factor eIF-2B beta... 82 1e-14
sp|Q28690|E2BB_RABIT TRANSLATION INITIATION FACTOR EIF-2B BETA S... 80 5e-14
pir||T38663 probable translation initiation factor eif-2b beta s... 79 6e-14
gi|6323322 translation initiation factor eIF2b, 43 kDa subunit; ... 77 3e-13
>sp|Q9V281|E2B2_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447876|pir||F75208
translation initiation factor aif-2bII translation
initiation factor PAB2402 - Pyrococcus abyssi (strain
Orsay) >gi|5457626|emb|CAB49117.1| (AJ248283)
translation initiation factor aIF-2BII translation
initiation factor [Pyrococcus abyssi]
Length = 324
Score = 649 bits (1655), Expect = 0.0
Identities = 324/324 (100%), Positives = 324/324 (100%)
Query: 1 MGAMIVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKI 60
MGAMIVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKI
Sbjct: 1 MGAMIVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKI 60
Query: 61 LYKTRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEI 120
LYKTRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEI
Sbjct: 61 LYKTRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEI 120
Query: 121 GAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIP 180
GAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIP
Sbjct: 121 GAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIP 180
Query: 181 VIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYK 240
VIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYK
Sbjct: 181 VIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYK 240
Query: 241 FHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIP 300
FHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIP
Sbjct: 241 FHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIP 300
Query: 301 PYAAIDILKEEFGWALKYREPWED 324
PYAAIDILKEEFGWALKYREPWED
Sbjct: 301 PYAAIDILKEEFGWALKYREPWED 324
>sp|O57947|E2B2_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447865|pir||F71243
probable translation initiation factor eIF-2B delta -
Pyrococcus horikoshii >gi|3256594|dbj|BAA29277.1|
(AP000001) 324aa long hypothetical translation
initiation factor eIF-2B delta [Pyrococcus horikoshii]
Length = 324
Score = 605 bits (1543), Expect = e-172
Identities = 297/324 (91%), Positives = 310/324 (95%)
Query: 1 MGAMIVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKI 60
MGAMIV+EVYE AEKIKSMEIRGAG+IAR+ AQALMIQAEKS AK PEELW ELK A+KI
Sbjct: 1 MGAMIVKEVYETAEKIKSMEIRGAGRIARAAAQALMIQAEKSKAKEPEELWNELKVASKI 60
Query: 61 LYKTRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEI 120
LY TRPTAVSLPNALRYVMHR K AY GADLETLRFT INSAKEFI+NSEKAIERIGEI
Sbjct: 61 LYNTRPTAVSLPNALRYVMHRVKAAYLGGADLETLRFTAINSAKEFIYNSEKAIERIGEI 120
Query: 121 GAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIP 180
GAKRIEDGD+IMTHCHSKAAISVMK AF+QGK+IKVIVTETRP+WQGKITAKELASYGIP
Sbjct: 121 GAKRIEDGDIIMTHCHSKAAISVMKKAFEQGKNIKVIVTETRPKWQGKITAKELASYGIP 180
Query: 181 VIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYK 240
VIY+VDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYK
Sbjct: 181 VIYIVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYK 240
Query: 241 FHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIP 300
FHP TMLGQLVEIEMRDPTEVIPEEEL+TWPKNIEVWNPAFDVTPPEYIDVIITE+GIIP
Sbjct: 241 FHPATMLGQLVEIEMRDPTEVIPEEELRTWPKNIEVWNPAFDVTPPEYIDVIITERGIIP 300
Query: 301 PYAAIDILKEEFGWALKYREPWED 324
PYAAIDILKEEFGWALKY+EPWED
Sbjct: 301 PYAAIDILKEEFGWALKYKEPWED 324
>sp|Q57586|E2B2_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|2129320|pir||B64315
translation initiation factor eIF-2B delta chain homolog
- Methanococcus jannaschii >gi|1590893|gb|AAB98103.1|
(U67469) translation initiation factor aIF-2B, subunit
delta, putative [Methanococcus jannaschii]
Length = 308
Score = 318 bits (807), Expect = 5e-86
Identities = 173/313 (55%), Positives = 222/313 (70%), Gaps = 14/313 (4%)
Query: 5 IVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKT 64
I++E YE KIK+MEIRGAG+I R+ A+AL A K + + EE +++EA IL
Sbjct: 10 IIKETYE---KIKNMEIRGAGRIGRAAAKALKEYALKISHLNEEEFKNKMREAGNILISA 66
Query: 65 RPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKR 124
RPTAVSLPN ++YV+ G + E + VI A EFI++S KAIE IG+ GA R
Sbjct: 67 RPTAVSLPNVVKYVL--------KGLNEENPKERVIERADEFINSSLKAIENIGKFGANR 118
Query: 125 IEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYV 184
I+DGD I+THC+S+AAISV+KTA+D+GKDIKV TETRPR QG +TAK L YGI V +
Sbjct: 119 IKDGDTILTHCNSEAAISVIKTAYDEGKDIKVFCTETRPRNQGYLTAKTLYDYGIDVTLI 178
Query: 185 VDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPE 244
VDSA R+++K D VV+GAD+ITANG ++NKIGTS IAL A E RV + AAETYKFHP+
Sbjct: 179 VDSAVRYFIKEIDIVVVGADAITANGCLVNKIGTSQIALIANESRVPFLTAAETYKFHPK 238
Query: 245 TMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPPYAA 304
T++G+L+EIE R P EV E+ K I++ NPAFDVTP +YID IITE G+IPP A
Sbjct: 239 TIVGELIEIEERSPEEVAVFEDKY---KGIKIRNPAFDVTPAKYIDAIITEVGLIPPQGA 295
Query: 305 IDILKEEFGWALK 317
I+++ FGW K
Sbjct: 296 WYIIEKYFGWLEK 308
>gi|11499619 translation initiation factor eIF-2B, subunit delta (eif2BD)
[Archaeoglobus fulgidus]
>gi|3182986|sp|O28242|E2B2_ARCFU PUTATIVE TRANSLATION
INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447866|pir||D69504 translation
initiation factor eIF-2B, subunit delta (eif2BD) homolog
- Archaeoglobus fulgidus >gi|2648498|gb|AAB89217.1|
(AE000962) translation initiation factor eIF-2B, subunit
delta (eif2BD) [Archaeoglobus fulgidus]
Length = 313
Score = 310 bits (785), Expect = 2e-83
Identities = 173/310 (55%), Positives = 217/310 (69%), Gaps = 12/310 (3%)
Query: 6 VREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTR 65
+ EV E AEKI+SME+RGA +IAR A+ LM AEK+ S E+ E++ AA+ L TR
Sbjct: 1 MEEVLEAAEKIRSMEVRGAARIARFAAETLMKFAEKA---SDEKFDEEMRFAAETLLNTR 57
Query: 66 PTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRI 125
PTAVSL NA+ YVM SG +E R +VI A+EFI+ E A +IGEIG KRI
Sbjct: 58 PTAVSLYNAINYVMRY------SGESVEEKRQSVIRRAREFINWVETAQRKIGEIGEKRI 111
Query: 126 EDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVV 185
+DG +MTHC+S AA+SV+K A + GK ++VI TE+RPRWQG +T K+L GI V +V
Sbjct: 112 KDGYTVMTHCNSSAALSVIKKAHENGKRVEVIATESRPRWQGHLTVKQLREAGIEVTLIV 171
Query: 186 DSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPET 245
DSA R+++ D VV+GAD+ITANGA+INKIGTS IAL AKE RV M+AAETYKF P+T
Sbjct: 172 DSAVRYFINEVDCVVVGADTITANGALINKIGTSQIALAAKEARVPFMVAAETYKFSPKT 231
Query: 246 MLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPPYAAI 305
+ G+LV IE RD EV PEE LK ++V NPAFDVTP +YIDVIITE G IPP A
Sbjct: 232 LFGELVVIEERDAREVAPEEILKL---GVKVRNPAFDVTPRDYIDVIITEIGAIPPEMAY 288
Query: 306 DILKEEFGWA 315
++ E G+A
Sbjct: 289 IVITERLGYA 298
>gb|AAG20059.1| (AE005085) translation initiation factor eIF-2B subunit alpha;
Eif2ba [Halobacterium sp. NRC-1]
Length = 314
Score = 290 bits (735), Expect = 1e-77
Identities = 154/321 (47%), Positives = 208/321 (63%), Gaps = 8/321 (2%)
Query: 4 MIVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYK 63
M+ +V A+ I MEIRGA IAR+ A AL QA S A SP EL+ AA+ L
Sbjct: 1 MVTEDVRATADDIADMEIRGAATIARAAADALESQATDSDADSPAAFRAELRAAARHLRD 60
Query: 64 TRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAK 123
TRPTAVSLPNALRYV+ + G + LR +V ++A F ++A + +G IGA
Sbjct: 61 TRPTAVSLPNALRYVLGN-----TDGETVAALRASVTDAADAFRQQLDRAQDDLGAIGAN 115
Query: 124 RIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIY 183
R+ DGD +MTHCHS A++ +K A D GK I+ +V ETRPR QG ITA +L +G+PV
Sbjct: 116 RLRDGDTVMTHCHSTDALACVKAALDDGKHIEAVVKETRPRKQGHITADQLREWGVPVTL 175
Query: 184 VVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHP 243
+VD+A R Y+ D V++GADSI A+G+VINKIGTS++A+ A+E V VM+AA+T K HP
Sbjct: 176 IVDNAGRRYLDAVDHVLVGADSIAADGSVINKIGTSMLAVVARERGVPVMVAAQTLKLHP 235
Query: 244 ETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPPYA 303
+T+ G VEIEMRD TEV+ + +V NPAFDVTPP Y+D I+TE+G PP +
Sbjct: 236 DTLTGNTVEIEMRDETEVLAAADRHQLGAP-DVENPAFDVTPPRYVDAIVTERGQFPPES 294
Query: 304 AIDILKEEFGWALKYREPWED 324
+ +++E +G EPW +
Sbjct: 295 IVTLMRELYGDGT--TEPWAE 313
>sp|Q57896|E2B1_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|2129325|pir||F64356
translation initiation factor aIF-2B alpha chain -
Methanococcus jannaschii >gi|1591158|gb|AAB98443.1|
(U67496) translation initiation factor aIF-2B, subunit
delta (aIF2BD) [Methanococcus jannaschii]
Length = 329
Score = 171 bits (429), Expect = 1e-41
Identities = 117/308 (37%), Positives = 168/308 (53%), Gaps = 32/308 (10%)
Query: 5 IVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKT 64
I + ++A IK M +RGA I S A L + E ++ +A ++L T
Sbjct: 33 ICKTYEDVAYAIKDMVVRGAPAIGVSAAYGLALA---------EINGDDIYKAYEVLKNT 83
Query: 65 RPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKR 124
RPTAV+L + + R AY G ++++ AK+ + ++IG IG K
Sbjct: 84 RPTAVNL----FWALDRCLTAYKEGK-------SILDEAKKIHEEDIETCKKIGMIGEKL 132
Query: 125 IEDGDVIMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELA 175
IEDGD I+THC++ A A+SV++ AF GK I+VI ETRPR QG K+TA EL
Sbjct: 133 IEDGDTILTHCNAGALATSAYGTALSVIRFAFYNGKKIRVIADETRPRLQGAKLTAFELN 192
Query: 176 SYGIPVIYVVDSAARHYMKM--TDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVM 233
GIPV + D+ A M+ DK+++GAD I A+G V NKIGT +A+ AK HR+
Sbjct: 193 YEGIPVKVITDNTAGFLMQKGEIDKIIVGADRILADGTVYNKIGTYSLAVLAKYHRIPFY 252
Query: 234 IAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVII 293
+AA F + ++ IE RD EV + ++ P+ + +N AFD TPP+ I II
Sbjct: 253 VAAPLSTFDLRSSEEDVI-IEERDEKEVAYIDGVRIVPEGVGCYNYAFDKTPPDLITAII 311
Query: 294 TEKGIIPP 301
TEKGI+ P
Sbjct: 312 TEKGIVKP 319
>gi|11497982 translation initiation factor eIF-2B, subunit delta (eif2BD)
[Archaeoglobus fulgidus]
>gi|3182987|sp|O29877|E2B1_ARCFU PUTATIVE TRANSLATION
INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447868|pir||B69296 translation
initiation factor eIF-2B, subunit delta (eif2BD) homolog
- Archaeoglobus fulgidus >gi|2650263|gb|AAB90865.1|
(AE001079) translation initiation factor eIF-2B, subunit
delta (eif2BD) [Archaeoglobus fulgidus]
Length = 339
Score = 169 bits (423), Expect = 5e-41
Identities = 113/308 (36%), Positives = 171/308 (54%), Gaps = 18/308 (5%)
Query: 7 REVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRP 66
R V E+A+ IK + +RGA + + A + + A + +EL LK+AA L TRP
Sbjct: 28 RNVEELADAIKKLAVRGAPALEAAGAYGIALAAREREFADVDELKEHLKKAADFLASTRP 87
Query: 67 TAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIE 126
TAV+L + RA A G +E ++ + A++ + ++GE GA+ +E
Sbjct: 88 TAVNLFVGIE----RALNAALKGESVEEVKELALREAEKLAEEDVERNRKMGEYGAELLE 143
Query: 127 DGDVIMTHCHSK--------AAISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASY 177
DGDV++T+C++ A+ V+++A +QGK+I+VI ETRP QG ++T EL
Sbjct: 144 DGDVVLTYCNAGRLATVDWGTALGVVRSAVEQGKEIRVIACETRPLNQGSRLTCWELMED 203
Query: 178 GIPVIYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIA 235
GI V + DS M+ M DKV++GAD I + AV NKIGT +++ AK H + +A
Sbjct: 204 GIDVTLITDSMVGIVMQKGMVDKVIVGADRIVRD-AVFNKIGTYTVSVVAKHHNIPFYVA 262
Query: 236 AETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITE 295
A F E +V IE R E+I + + P N++V+NPAFD TP E + +ITE
Sbjct: 263 APKATFDWERTAKDVV-IEERPREELIFCGKRQIAPLNVKVYNPAFDPTPLENVTALITE 321
Query: 296 KGII-PPY 302
G+I PPY
Sbjct: 322 YGVIYPPY 329
>sp|Q9YE84|E2B1_AERPE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447874|pir||F72657
probable translation initiation factor eIF-2B alpha
subunit APE0686 [imported] - Aeropyrum pernix (strain
K1) >gi|5104347|dbj|BAA79662.1| (AP000060) 370aa long
hypothetical translation initiation factor eFI-2B alpha
subunit [Aeropyrum pernix]
Length = 370
Score = 165 bits (414), Expect = 5e-40
Identities = 115/323 (35%), Positives = 170/323 (52%), Gaps = 24/323 (7%)
Query: 7 REVYE-------IAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAK 59
REVY +A I MEIRGA I + A AL + A ++ ++ E L EA +
Sbjct: 44 REVYRRTSDYRRVARAIVDMEIRGAPAIGVAAAYALALAAAEAASRGGEGFIEALSEARR 103
Query: 60 ILYKTRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGE 119
+ TRPTA +L A V A+ +R + + + ++ + +E IG
Sbjct: 104 EIESTRPTAYNLFWATARVYGAVSEAFRRSGVDAAVRAGLEEATRIYVEDVRGNVE-IGR 162
Query: 120 IGAKRIEDGDVIMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KIT 170
+GA+ +E GD ++THC++ A A+ V++ A+ +GKDI+VI TETRP QG ++
Sbjct: 163 VGARLLESGDTVLTHCNTGALATAGFGTALGVIRYAWMEGKDIRVITTETRPVLQGARLN 222
Query: 171 AKELASYGIPVIYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEH 228
EL GIP VVDSA M M K ++GAD I + G NKIGT ++A+ A H
Sbjct: 223 VWELRKEGIPFKLVVDSAVGLIMSRGMVSKAIVGADRIVSTGHTANKIGTYMVAMAASRH 282
Query: 229 RVWVMIAAETYKFHPETMLGQLVEIEMRDPTE---VIPEE-ELKTWPKNIEVWNPAFDVT 284
V +AA F P+ + + IE R P E VI E ++ ++E +NP+FDVT
Sbjct: 283 GVPFYVAAPASTFQPDAG-PEAIVIEERSPDEVRGVISEAGYVRITLGDVEAYNPSFDVT 341
Query: 285 PPEYIDVIITEKGIIPPYAAIDI 307
PPE I IT++G+I P ++I
Sbjct: 342 PPELITAFITDRGVIEPPFDVNI 364
>sp|P74497|E2B_SYNY3 PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447869|pir||S76472 hypothetical
protein - Synechocystis sp. (strain PCC 6803)
>gi|1653689|dbj|BAA18601.1| (D90915) hypothetical
protein [Synechocystis sp.]
Length = 351
Score = 160 bits (401), Expect = 2e-38
Identities = 108/306 (35%), Positives = 160/306 (51%), Gaps = 17/306 (5%)
Query: 16 IKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNAL 75
IKSM +RGA I + A + + A + E +L+ A L +TRPTAV+L A+
Sbjct: 47 IKSMVVRGAPAIGVAAAYGMYLGARRIQTTELREFVVQLEFVADQLRQTRPTAVNLFWAI 106
Query: 76 RYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAK---RIEDGDVIM 132
+++ +AY SG ++E ++ +++ A++ + + + IG G + R I+
Sbjct: 107 DQMLN---VAYHSGENVEQIKANLLSQAQQIQLDDLRTCQAIGAAGLEVLPREPHQLTIL 163
Query: 133 THCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASYGIPVIY 183
THC++ A AI V++ A+ G+ +V ETRPR QG K+T E GIPV
Sbjct: 164 THCNAGALATAGYGTAIGVIRAAWSAGRLARVYADETRPRLQGAKLTVWECVQAGIPVTL 223
Query: 184 VVDSAARHYMKMT--DKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKF 241
+ D+ A H M+ D VV+GAD I NG + NKIGT +A+ AK H + +AA
Sbjct: 224 ITDNMAAHCMQQQRIDAVVVGADRIARNGDIANKIGTYGLAVLAKMHAIPFFVAAPLSTV 283
Query: 242 HPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPP 301
G + IE RDP E+ E + P+ E +NPAFDVTP I IITE+G I P
Sbjct: 284 DFALDDGSQIPIEERDPVEIYQPEGNRITPEGAEFYNPAFDVTPASLITAIITEQGAIAP 343
Query: 302 YAAIDI 307
D+
Sbjct: 344 EKLADL 349
>sp|Q9X013|E2B_THEMA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447871|pir||F72319 translation
initiation factor IF-2B alpha subunit-related TM0911
[similarity] - Thermotoga maritima (strain MSB8)
>gi|4981447|gb|AAD35992.1|AE001755_15 (AE001755)
translation initiation factor, aIF-2B alpha
subunit-related [Thermotoga maritima]
Length = 343
Score = 158 bits (395), Expect = 9e-38
Identities = 109/302 (36%), Positives = 158/302 (52%), Gaps = 18/302 (5%)
Query: 11 EIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVS 70
E+A IK M +RGA I + A ++ S + ++KE L +TRPTAV+
Sbjct: 37 EVAHAIKEMIVRGAPAIGVAAAFGYVLGLRDYKTGSLTDWMKQVKET---LARTRPTAVN 93
Query: 71 LPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDV 130
L + ++R + + AD E L + N A + + + + IG+ GA+ I+DG
Sbjct: 94 L----FWALNRMEKVFFENADRENLFEILENEALKMAYEDIEVNKAIGKNGAQLIKDGST 149
Query: 131 IMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASYGIPV 181
I+THC++ A A+ V++ A + GK I+V ETRP QG ++TA EL GI V
Sbjct: 150 ILTHCNAGALATVDYGTALGVIRAAVESGKRIRVFADETRPYLQGARLTAWELMKDGIEV 209
Query: 182 IYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETY 239
+ D+ A MK + D VV+GAD I NG NKIGT +A+ AK + + +AA
Sbjct: 210 YVITDNMAGWLMKRGLIDAVVVGADRIALNGDTANKIGTYSLAVLAKRNNIPFYVAAPVS 269
Query: 240 KFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGII 299
P G+ + IE R P EV + P+ ++V NPAFDVT I IITEKG+I
Sbjct: 270 TIDPTIRSGEEIPIEERRPEEVTHCGGNRIAPEGVKVLNPAFDVTENTLITAIITEKGVI 329
Query: 300 PP 301
P
Sbjct: 330 RP 331
>sp|Q9PAG5|E2B_XYLFA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|11279300|pir||A82544 translation
initiation factor IF-2B alpha subunit-related XF2553
[imported] - Xylella fastidiosa (strain 9a5c)
>gi|9107764|gb|AAF85350.1|AE004062_5 (AE004062)
initiation factor eIF-2B, alpha subunit-related [Xylella
fastidiosa]
Length = 354
Score = 156 bits (391), Expect = 3e-37
Identities = 100/300 (33%), Positives = 151/300 (50%), Gaps = 17/300 (5%)
Query: 11 EIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVS 70
++ + I+++ +RGA I ++ A + A E +L+ A + L+ RPTAV+
Sbjct: 47 DVTQAIRALIVRGAPAIGIVAGWGAVLAAREIEAVDGIEALRKLEPALQRLHAARPTAVN 106
Query: 71 LPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDV 130
L L + A++ R + A+ A +G GA I G
Sbjct: 107 LAWVLARMRRTLSAAHADW------RQAMAREAESIAREDLTANRCMGAYGAALIPIGSG 160
Query: 131 IMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASYGIPV 181
++THC++ + A+ V++ QG+ +V ETRP QG ++T EL GI V
Sbjct: 161 VLTHCNTGSLATAGFGTALGVIRAGIAQGRIARVFAGETRPWLQGARLTVWELQQDGIDV 220
Query: 182 IYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETY 239
+ DSAA H MK V++GAD I ANG NKIGT +A+TA+ H V M+ A
Sbjct: 221 TLIADSAAAHLMKSGQVQWVIVGADRICANGDAANKIGTYQLAITARHHGVKFMVVASAA 280
Query: 240 KFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGII 299
+T G+ +EIE RDP E++ ++T + I WNP FDVTP ID I+TE+G+I
Sbjct: 281 TVDMDTTAGEAIEIEQRDPEELLGVSGVRTVAEGIAAWNPVFDVTPGALIDAIVTERGVI 340
>sp|O58185|E2BL_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2-LIKE
(EIF-2B GDP-GTP EXCHANGE FACTOR) >gi|7521497|pir||A71155
probable translation initiation factor eIF-2B -
Pyrococcus horikoshii >gi|3256843|dbj|BAA29526.1|
(AP000002) 276aa long hypothetical translation
initiation factor eIF-2B [Pyrococcus horikoshii]
Length = 276
Score = 154 bits (386), Expect = 1e-36
Identities = 100/298 (33%), Positives = 155/298 (51%), Gaps = 27/298 (9%)
Query: 4 MIVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYK 63
M+ V EI ++K I+GA +A+ A+A + AE+ E+ EL+E + K
Sbjct: 1 MLPERVLEILREMKRERIKGASWLAKKGAEAFLTLAEELDESLLEDAIMELREE---VVK 57
Query: 64 TRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAK 123
P+ SL N R++ + R + + A EF+ E+A + IGA+
Sbjct: 58 VNPSMASLYNLARFI------------PVTNRRDILKSRALEFLRRMEEAKRELASIGAQ 105
Query: 124 RIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIY 183
I+DGDVI+TH S + +++TA ++ K KVI+TE+ P ++G A+EL GI
Sbjct: 106 LIDDGDVIITHSFSSTVLEIIRTAKERKKRFKVILTESSPDYEGLHLARELEFSGIEFEV 165
Query: 184 VVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHP 243
+ D+ + + ++GAD IT +G V+NK GT L+AL E+ + +AAETYKFHP
Sbjct: 166 ITDAQMGLFCREASIAIVGADMITKDGYVVNKAGTYLLALACHENAIPFYVAAETYKFHP 225
Query: 244 ETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPP 301
G ++ +E RD N+ + N FDVTP +Y+ IITE GI+ P
Sbjct: 226 TLKSGDVMLME-RDLIR-----------GNVRIRNVLFDVTPWKYVRGIITELGIVIP 271
>sp|O67879|E2B_AQUAE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7519844|pir||C70481 translation
initiation factor eIF-2B alpha chain - Aquifex aeolicus
>gi|2984318|gb|AAC07840.1| (AE000773) initiation factor
eIF-2B alpha subunit [Aquifex aeolicus]
Length = 456
Score = 153 bits (384), Expect = 2e-36
Identities = 116/311 (37%), Positives = 159/311 (50%), Gaps = 30/311 (9%)
Query: 11 EIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVS 70
E+A+ IK M +RGA I + A ++ K + PE+++ LK TRPTA +
Sbjct: 165 EVAKAIKEMAVRGAPAIGCTAAYGFVLGV-KVQKEDPEKVYETLKN-------TRPTAYN 216
Query: 71 LPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDV 130
L AL +M K G D+E AKE +A +R+GEIG++ +
Sbjct: 217 LFWALDRMMKALK----EGKDIE-------EEAKEIEKEDYEANKRMGEIGSEIVPLNAK 265
Query: 131 IMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASYGIPV 181
++THC++ A A+ V+++A GK+I V V ETRP QG ++TA EL IP
Sbjct: 266 VLTHCNTGALATAGWGTALGVIRSAHYGGKNIFVWVDETRPYLQGARLTAWELVKESIPH 325
Query: 182 IYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETY 239
+ DS A MK M D V++GAD ITA G V NKIGT +A+ KEH V +AA T
Sbjct: 326 KIITDSTAGFLMKKGMVDLVIVGADRITAKGDVANKIGTYTLAVLCKEHNVPFYVAAPTS 385
Query: 240 KFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGII 299
G+ + IE R P EV PK + + AFD+TP E I IITEKGI
Sbjct: 386 TIDSNIKSGEEIIIEERSPEEVKVCGGCAIAPKESDALHLAFDITPAELITGIITEKGIF 445
Query: 300 PPYAAIDILKE 310
P + LK+
Sbjct: 446 KPERITEALKD 456
>sp|O58433|E2B1_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447867|pir||G71116
probable translation initiation factor eIF-2 beta chain
- Pyrococcus horikoshii >gi|3257110|dbj|BAA29793.1|
(AP000003) 364aa long hypothetical translation
initiation factor eIF-2 beta chain [Pyrococcus
horikoshii]
Length = 364
Score = 152 bits (379), Expect = 7e-36
Identities = 105/307 (34%), Positives = 166/307 (53%), Gaps = 16/307 (5%)
Query: 8 EVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPT 67
+V E+AE IK+M +RGA I + L + AE S AK+ EE ++A +IL TRPT
Sbjct: 43 KVEEVAEAIKNMTVRGAPAIGAAAGFGLALYAETSKAKTKEEFLDGFEKAYEILKNTRPT 102
Query: 68 AVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIED 127
AV+L AL + + + S L+ ++ ++ A + +A R+G GA+ + +
Sbjct: 103 AVNLFWALNRI--KKLVEEHSEDPLDEIKRLIVQEAYKIADEDVEANLRMGHYGAEVLPE 160
Query: 128 GDVIMTHC--------HSKAAISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASYG 178
G+ I+THC H SV++ G + + ETRP QG +++A E + G
Sbjct: 161 GN-ILTHCNAGSLATVHLGTVGSVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDG 219
Query: 179 IPVIYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAA 236
+ V + D+AA M+ D +++GAD I ANG NKIGT ++A+ A+EH + A
Sbjct: 220 LNVKLIADNAAAFVMQQGFVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVA 279
Query: 237 ETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEK 296
E G+ + IE R P EV+ + P ++ V+NPAFDVTP +Y+ IIT++
Sbjct: 280 PLSSIDMELKSGKDIPIEERSPEEVLTCGGCRIAP-DVPVYNPAFDVTPHKYLTGIITDR 338
Query: 297 GII-PPY 302
G++ PP+
Sbjct: 339 GVVWPPF 345
>sp|Q9UYB6|E2BL_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2-LIKE
(EIF-2B GDP-GTP EXCHANGE FACTOR) >gi|7521496|pir||B75007
probable translation initiation factor aif-2bII
translation initiation factor PAB1306 - Pyrococcus
abyssi (strain Orsay) >gi|5459010|emb|CAB50496.1|
(AJ248288) putative, translation initiation factor
aIF-2BII translation initiation factor [Pyrococcus
abyssi]
Length = 276
Score = 147 bits (368), Expect = 1e-34
Identities = 101/298 (33%), Positives = 153/298 (50%), Gaps = 27/298 (9%)
Query: 4 MIVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYK 63
M+ V EI ++K+ IRGA +AR A+A + AE+ E+ EL+ + +
Sbjct: 1 MLPDRVLEILNEMKAERIRGATWLARKGAEAFLALAEELDEALLEDAIRELRSR---IIE 57
Query: 64 TRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAK 123
P+ SL N R++ ++ +L +R A EF+ ++A + IGA+
Sbjct: 58 VNPSMASLYNLARFM------PITNNRELVKMR------ALEFLRRMDEAKRELASIGAQ 105
Query: 124 RIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIY 183
I+DGDVI+TH S + + + K A D+ K KVI+TE+ P ++G A EL + GI
Sbjct: 106 LIDDGDVIITHSFSSSVLEIFKVAKDRRKSFKVIITESSPDYEGLHLANELENLGIEFEV 165
Query: 184 VVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHP 243
+ DS + + ++GAD +T +G V+NK GT L+AL E V +AAETYKFHP
Sbjct: 166 ITDSQMGLFCRKATISMVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHP 225
Query: 244 ETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPP 301
T+ V + RD + V N FD+TP ++I IITE GI+ P
Sbjct: 226 -TVKSNEVVLHERDFSR-----------SGFRVRNVLFDLTPWKFIRGIITELGIVIP 271
>sp|Q9UZ16|E2B1_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447875|pir||A75044
translation initiation factor aif-2bi PAB2444 -
Pyrococcus abyssi (strain Orsay)
>gi|5458759|emb|CAB50246.1| (AJ248287) translation
initiation factor aIF-2BI [Pyrococcus abyssi]
Length = 356
Score = 147 bits (368), Expect = 1e-34
Identities = 106/319 (33%), Positives = 169/319 (52%), Gaps = 18/319 (5%)
Query: 7 REVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRP 66
R V +AE IK+M +RGA I + A L + AE S AKS +E +A + L TRP
Sbjct: 42 RTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRP 101
Query: 67 TAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIE 126
TAV+L AL + + + L+ ++ ++N A++ +A R+G GA+ +
Sbjct: 102 TAVNLFWALNRI--KKLVEEHLEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLP 159
Query: 127 DGDVIMTHC--------HSKAAISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASY 177
+G+ ++THC H +V++ G + + ETRP QG +++A E +
Sbjct: 160 EGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYD 218
Query: 178 GIPVIYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIA 235
G+ V + D+AA M+ + D +++GAD I ANG NKIGT ++A+ A+EH +
Sbjct: 219 GLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAV 278
Query: 236 AETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITE 295
A G+ + IE R P EV+ + P ++ V+NPAFDVTP +Y+ IIT+
Sbjct: 279 APLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIAP-DVPVYNPAFDVTPHKYVTGIITD 337
Query: 296 KGII-PPYAAIDILKEEFG 313
K ++ PP+ LK+ FG
Sbjct: 338 KRVVWPPFKR--NLKKLFG 354
>gb|AAK43082.1| Translation initiation factor aiF-2B, subunit 1 (eiF2B) [Sulfolobus
solfataricus]
Length = 366
Score = 146 bits (366), Expect = 2e-34
Identities = 108/313 (34%), Positives = 161/313 (50%), Gaps = 17/313 (5%)
Query: 6 VREVYEIAEKIKSMEIRGAGKIARSVAQALMIQ-AEKSTAKSPEELWTELKEAAKILYKT 64
+++V A IK+M++RGA I + +++ K+ +E EL A IL
Sbjct: 45 LKDVDSTALAIKNMQVRGAPAIGITAGYGMVLALTSNKNVKTLDEAIRELTRAKAILDSA 104
Query: 65 RPTAVSLPNALRYVMHRAKIAYSSG--ADLETLRFTVINSAKEFIHNSEKAIERIGEIGA 122
RPTAV+L A ++ AK A +G + L + AK A ++G G
Sbjct: 105 RPTAVNLVWATSRMLTLAKNAVENGNAKTINELIDLMKVEAKRIFDEEYDAEIQMGLYGL 164
Query: 123 KRIEDGDVIMTHCHSKA---------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAK 172
+++ D D ++T C++ A+S +K A G ++ VI ETRP QG ++T
Sbjct: 165 EKLNDSDTVLTQCNAGGLATGTGLGTALSPVKLAKALGMNVSVIAPETRPWLQGSRLTVY 224
Query: 173 ELASYGIPVIYVVDSAAR--HYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRV 230
EL GI V + D+A Y M + V++GAD I +G V NKIGT A+ A E +
Sbjct: 225 ELMEEGIKVTLITDTAVGLVMYRGMVNSVMVGADRILRDGHVFNKIGTYKEAVIAHELGI 284
Query: 231 WVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYID 290
A T F ++ + + ++IE RDP EV + P+N+ V+NP FDVTPP+YI
Sbjct: 285 PFYALAPTSSFDLKSNVNE-IKIEERDPNEVRTIRGIPIAPENVNVYNPVFDVTPPKYIA 343
Query: 291 VIITEKGII-PPY 302
IITEKGII PP+
Sbjct: 344 GIITEKGIIYPPF 356
>sp|O27900|E2B1_METTH PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447870|pir||E69117
translation initiation factor eIF-2B, alpha subunit -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2623009|gb|AAB86338.1| (AE000939) translation
initiation factor eIF-2B, alpha subunit
[Methanobacterium thermoautotrophicum]
Length = 311
Score = 145 bits (363), Expect = 5e-34
Identities = 107/306 (34%), Positives = 158/306 (50%), Gaps = 37/306 (12%)
Query: 7 REVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRP 66
R+ ++ IK+M +RGA I + A + + E +++ AA+ + +RP
Sbjct: 31 RDYRDVIYAIKNMVVRGAPAIGVTAAFGVAMA---------ELAGEDVEVAAEEIKASRP 81
Query: 67 TAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIE 126
TAV+L A+ VM + ++ A + IG GA IE
Sbjct: 82 TAVNLFWAVDRVMKSE---------------SPLDEAITMYMEDMETNRAIGRHGAGLIE 126
Query: 127 DGDVIMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASY 177
DGD ++THC++ A A+ V++ A+D GK + VI ETRP QG +++ E+
Sbjct: 127 DGDTVLTHCNAGALACVDYGTALGVIRAAWDDGKRLTVICDETRPVGQGARLSVWEMQQE 186
Query: 178 GIPVIYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIA 235
GIPV + D AA + M+ M DKV++GAD I A G V NKIG+ ++AL AK V +A
Sbjct: 187 GIPVKLITDVAAGYLMQTGMIDKVIIGADRI-AEGGVANKIGSLMVALAAKRFNVPFYVA 245
Query: 236 AETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITE 295
A F E + +EIE RDP EV+ + P++ E NPAFD+ P + I IITE
Sbjct: 246 APMSTFDTENSIYD-IEIEERDPAEVLYYGGCRIAPQDTEAINPAFDIVPSDLISGIITE 304
Query: 296 KGIIPP 301
KGI+ P
Sbjct: 305 KGILDP 310
>emb|CAC12337.1| (AL445066) translation initiation factor aIF-2B, alpha chain
related protein [Thermoplasma acidophilum]
Length = 340
Score = 145 bits (362), Expect = 7e-34
Identities = 109/302 (36%), Positives = 148/302 (48%), Gaps = 35/302 (11%)
Query: 11 EIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVS 70
+IA IK+M +RGA I + A L + +K + EA + + TRPTA
Sbjct: 44 DIAYAIKNMVVRGAPAIGVTAAYGLAMAKKKGE---------NMDEAVRKIGSTRPTAYD 94
Query: 71 LPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDV 130
L A+RY+ D + +NSA+ + +IGEIG + I
Sbjct: 95 LFKAIRYMQIN---------DFD------MNSARRYAMEISGRSRKIGEIGNQLIGQNAR 139
Query: 131 IMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASYGIPV 181
I+THC++ A A++ ++ A GK+I V V ETRPR QG K+TA ELA GI
Sbjct: 140 ILTHCNAGALAVVDWGTALAPIRMAHGDGKNIFVFVDETRPRLQGAKLTAWELAQEGIDH 199
Query: 182 IYVVDSAARHYM--KMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETY 239
+ D+AA YM K D V++GAD I +NG NKIGT A+ AK + + +AA
Sbjct: 200 AIIADNAAGFYMRRKEVDLVIVGADRIASNGDFANKIGTYEKAVLAKTNGIPFYVAAPGS 259
Query: 240 KFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGII 299
F G + IE RD EV+ + P NPAFDVTP EY+ ITE GI
Sbjct: 260 TFDFSIKSGDEIPIEERDENEVLEINGTRIGPVESHSRNPAFDVTPNEYVTGFITEYGIF 319
Query: 300 PP 301
P
Sbjct: 320 KP 321
>pir||A83249 probable initiation factor 2 subunit PA3169 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9949286|gb|AAG06557.1|AE004741_2 (AE004741) probable
initiation factor 2 subunit [Pseudomonas aeruginosa]
Length = 358
Score = 142 bits (354), Expect = 6e-33
Identities = 107/306 (34%), Positives = 163/306 (52%), Gaps = 25/306 (8%)
Query: 11 EIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELW-TELKEAAKILYKTRPTAV 69
E+A+ I+ M +RGA I S A +++ A A+ + W L+E ++L +RPTAV
Sbjct: 43 EVAKAIRDMVVRGAPAIGISAAYGIVLGARARLAQGGD--WRAALEEDFRLLADSRPTAV 100
Query: 70 SLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEK----AIERIG-EIGAKR 124
+L AL + R + L L I+ IH S++ + ++G E+ K+
Sbjct: 101 NLFWALNRMRDRLERMKEGDQPLAVLEAEAIS-----IHESDREANLTMAQLGMELIRKQ 155
Query: 125 IEDGDVIMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELA 175
I+THC++ A A+ V++ A +G ++ ETRP QG ++TA ELA
Sbjct: 156 QGSPQNILTHCNTGALATGGFGTALGVIRAAHLEGLVNRIYADETRPWLQGSRLTAWELA 215
Query: 176 SYGIPVIYVVDSAARHYMKMTDK--VVMGADSITANGAVINKIGTSLIALTAKEHRVWVM 233
+ GIPV VDSAA H MK + V++GAD ITANG V NKIGT +A+ A H V M
Sbjct: 216 NEGIPVSLNVDSAAAHLMKTENITWVIVGADRITANGDVANKIGTYQLAVNAMHHGVRFM 275
Query: 234 IAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVII 293
+ A + G+ + IE RD E++ E + ++ +NP FDVTP + ID I+
Sbjct: 276 VVAPSSTIDMNLESGEDIPIEERDGRELL-EIGGRRVAAEVDAYNPVFDVTPADLIDAIV 334
Query: 294 TEKGII 299
TE+G++
Sbjct: 335 TERGVV 340
>sp|O31662|E2B_BACSU PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447872|pir||C69863 translation
initiation factor IF-2B alpha chain homolog ykrS
[similarity] - Bacillus subtilis
>gi|2633726|emb|CAB13228.1| (Z99111) similar to
initiation factor eIF-2B (alpha subunit) [Bacillus
subtilis]
Length = 353
Score = 139 bits (347), Expect = 4e-32
Identities = 96/300 (32%), Positives = 151/300 (50%), Gaps = 15/300 (5%)
Query: 11 EIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVS 70
++ + I ++++RGA I + A L + A+ + E L++ + L +RPTA++
Sbjct: 40 DVFDAIVTLKVRGAPAIGITAAFGLALAAKDIETDNVTEFRRRLEDIKQYLNSSRPTAIN 99
Query: 71 LPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDV 130
L AL + H + A S + + +++ A + E+ IG+ + + GD
Sbjct: 100 LSWALERLSHSVENAIS----VNEAKTNLVHEAIQIQVEDEETCRLIGQNALQLFKKGDR 155
Query: 131 IMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASYGIPV 181
IMT C++ + A++ A + + + ETRP QG ++TA EL GI V
Sbjct: 156 IMTICNAGSIATSRYGTALAPFYLAKQKDLGLHIYACETRPVLQGSRLTAWELMQGGIDV 215
Query: 182 IYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETY 239
+ DS A H MK V++GAD I NG NKIGT +A+ A + +AA
Sbjct: 216 TLITDSMAAHTMKEKQISAVIVGADRIAKNGDTANKIGTYGLAILANAFDIPFFVAAPLS 275
Query: 240 KFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGII 299
F + G + IE RDP EV ++T P N+ V+NPAFD+TP + I IITEKGI+
Sbjct: 276 TFDTKVKCGADIPIEERDPEEVRQISGVRTAPSNVPVFNPAFDITPHDLISGIITEKGIM 335
>sp|Q63186|E2BD_RAT TRANSLATION INITIATION FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|1083768|pir||S52373 translation
initiation factor eIF-2B delta chain - rat
>gi|7514109|pir||S71961 translation initiation factor
eIF-2B delta chain - rat >gi|667056|emb|CAA88256.1|
(Z48225) protein synthesis initiation factor eIF-2B
delta subunit [Rattus norvegicus]
Length = 524
Score = 138 bits (343), Expect = 1e-31
Identities = 91/292 (31%), Positives = 153/292 (52%), Gaps = 18/292 (6%)
Query: 24 AGKIARSVA--QALMIQAEKSTAKSPEELWTELKEAAK----ILYKTRPTAVSLPNALRY 77
+G AR +A AL + T EEL +L K L + RP + S+ NA+++
Sbjct: 221 SGSNARCIALLHALQQVIQDYTTPPNEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKF 280
Query: 78 VMHRAKIAYSSGADLET---LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTH 134
SS + E L+ + +E I + +AI R +K+I DGDVI+ +
Sbjct: 281 FNKEVTGMSSSKREEEAKSELKEAIDRYVQEKIVLASQAISRFA---SKKISDGDVILVY 337
Query: 135 CHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMK 194
S +++ A+ +G+ +V+V ++RPR +G+ L G+P Y++ AA + +
Sbjct: 338 GCSSLVSRILQEAWVEGRRFRVVVVDSRPRLEGRHMLHCLVRAGVPTSYLLIPAASYVLP 397
Query: 195 MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIE 254
KV++GA ++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E
Sbjct: 398 EVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNE 457
Query: 255 MRDPTEVI----PEEELKTWPKN--IEVWNPAFDVTPPEYIDVIITEKGIIP 300
+ DP ++ + L W N + + N +DVTPPE +D++ITE G+IP
Sbjct: 458 LDDPDDLQCKRGDQVTLANWQNNSSLRLLNLVYDVTPPELVDLVITELGMIP 509
>emb|CAA53204.1| (X75451) translation initiation factor eIF-2B-delta [Oryctolagus
cuniculus]
Length = 527
Score = 137 bits (342), Expect = 2e-31
Identities = 87/290 (30%), Positives = 155/290 (53%), Gaps = 14/290 (4%)
Query: 24 AGKIARSVA--QALMIQAEKSTAKSPEELWTELKEAAK----ILYKTRPTAVSLPNALRY 77
+G AR +A +AL + T EEL +L K L + RP + S+ NA+++
Sbjct: 224 SGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYICFLTQCRPLSASMYNAIKF 283
Query: 78 VMHRAKIAYSSGADLETLRFTVINSAKEFIHNS-EKAIERIGEIGAKRIEDGDVIMTHCH 136
+++ SS E + + +A ++ A + I +K+I +GDVI+ +
Sbjct: 284 -LNKEITGVSSTKREEEAKAELQAAADRYVQEKIVLAAQAILRFASKKISNGDVILVYGC 342
Query: 137 SKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMT 196
S +++ A+ +G+ +V+V ++RPR +G+ + L G+P Y++ AA + +
Sbjct: 343 SSLVSRILQEAWSEGRKFRVVVVDSRPRLEGRHMLRFLVRAGVPASYLLIPAASYVLPEV 402
Query: 197 DKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMR 256
KV++GA ++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E+
Sbjct: 403 SKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELD 462
Query: 257 DPTEVIPEE----ELKTWPK--NIEVWNPAFDVTPPEYIDVIITEKGIIP 300
DP ++ E L W ++ + N +DVTPPE +D++ITE G+IP
Sbjct: 463 DPDDLQCERGDHVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIP 512
>sp|P41111|E2BD_RABIT TRANSLATION INITIATION FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|631601|pir||S42727 translation
initiation factor eIF-2 delta chain - rabbit
Length = 523
Score = 137 bits (342), Expect = 2e-31
Identities = 87/290 (30%), Positives = 155/290 (53%), Gaps = 14/290 (4%)
Query: 24 AGKIARSVA--QALMIQAEKSTAKSPEELWTELKEAAK----ILYKTRPTAVSLPNALRY 77
+G AR +A +AL + T EEL +L K L + RP + S+ NA+++
Sbjct: 220 SGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYICFLTQCRPLSASMYNAIKF 279
Query: 78 VMHRAKIAYSSGADLETLRFTVINSAKEFIHNS-EKAIERIGEIGAKRIEDGDVIMTHCH 136
+++ SS E + + +A ++ A + I +K+I +GDVI+ +
Sbjct: 280 -LNKEITGVSSTKREEEAKAELQAAADRYVQEKIVLAAQAILRFASKKISNGDVILVYGC 338
Query: 137 SKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMT 196
S +++ A+ +G+ +V+V ++RPR +G+ + L G+P Y++ AA + +
Sbjct: 339 SSLVSRILQEAWSEGRKFRVVVVDSRPRLEGRHMLRFLVRAGVPASYLLIPAASYVLPEV 398
Query: 197 DKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMR 256
KV++GA ++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E+
Sbjct: 399 SKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELD 458
Query: 257 DPTEVIPEE----ELKTWPK--NIEVWNPAFDVTPPEYIDVIITEKGIIP 300
DP ++ E L W ++ + N +DVTPPE +D++ITE G+IP
Sbjct: 459 DPDDLQCERGDHVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIP 508
>pir||T08757 probable translation initiation factor eIF-2B delta chain - human
(fragment) >gi|4884138|emb|CAB43277.1| (AL050109)
hypothetical protein [Homo sapiens]
Length = 394
Score = 137 bits (342), Expect = 2e-31
Identities = 82/283 (28%), Positives = 150/283 (52%), Gaps = 12/283 (4%)
Query: 27 IARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAY 86
+ R++ Q + + +L +LK L + RP + S+ NA++++
Sbjct: 100 LLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVG 159
Query: 87 SSGADLET---LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISV 143
SS + E LR + +E I + +AI R ++I +GDVI+ + S +
Sbjct: 160 SSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAY---QKISNGDVILVYGCSSLVSRI 216
Query: 144 MKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGA 203
++ A+ +G+ +V+V ++RP +G+ T + L G+P Y++ AA + + KV++GA
Sbjct: 217 LQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGA 276
Query: 204 DSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIP 263
++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E+ DP ++
Sbjct: 277 HALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQC 336
Query: 264 EE----ELKTWPK--NIEVWNPAFDVTPPEYIDVIITEKGIIP 300
+ L W ++ + N +DVTPPE +D++ITE G+IP
Sbjct: 337 KRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIP 379
>emb|CAB57260.1| (AJ011305) guanine nucleotide exchange factor, delta subunit [Homo
sapiens] >gi|6012057|emb|CAB57304.1| (AJ011307) guanine
nucleotide exchange factor [Homo sapiens]
Length = 522
Score = 137 bits (342), Expect = 2e-31
Identities = 82/283 (28%), Positives = 150/283 (52%), Gaps = 12/283 (4%)
Query: 27 IARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAY 86
+ R++ Q + + +L +LK L + RP + S+ NA++++
Sbjct: 228 LLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVG 287
Query: 87 SSGADLET---LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISV 143
SS + E LR + +E I + +AI R ++I +GDVI+ + S +
Sbjct: 288 SSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAY---QKISNGDVILVYGCSSLVSRI 344
Query: 144 MKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGA 203
++ A+ +G+ +V+V ++RP +G+ T + L G+P Y++ AA + + KV++GA
Sbjct: 345 LQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGA 404
Query: 204 DSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIP 263
++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E+ DP ++
Sbjct: 405 HALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQC 464
Query: 264 EE----ELKTWPK--NIEVWNPAFDVTPPEYIDVIITEKGIIP 300
+ L W ++ + N +DVTPPE +D++ITE G+IP
Sbjct: 465 KRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIP 507
>emb|CAB57261.1| (AJ011306) guanine nucleotide exchange factor (long isoform) [Homo
sapiens]
Length = 543
Score = 137 bits (342), Expect = 2e-31
Identities = 82/283 (28%), Positives = 150/283 (52%), Gaps = 12/283 (4%)
Query: 27 IARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAY 86
+ R++ Q + + +L +LK L + RP + S+ NA++++
Sbjct: 249 LLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVG 308
Query: 87 SSGADLET---LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISV 143
SS + E LR + +E I + +AI R ++I +GDVI+ + S +
Sbjct: 309 SSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAY---QKISNGDVILVYGCSSLVSRI 365
Query: 144 MKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGA 203
++ A+ +G+ +V+V ++RP +G+ T + L G+P Y++ AA + + KV++GA
Sbjct: 366 LQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGA 425
Query: 204 DSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIP 263
++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E+ DP ++
Sbjct: 426 HALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQC 485
Query: 264 EE----ELKTWPK--NIEVWNPAFDVTPPEYIDVIITEKGIIP 300
+ L W ++ + N +DVTPPE +D++ITE G+IP
Sbjct: 486 KRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIP 528
>emb|CAB57305.1| (AJ011307) guanine nucleotide exchange factor [Homo sapiens]
Length = 542
Score = 137 bits (342), Expect = 2e-31
Identities = 82/283 (28%), Positives = 150/283 (52%), Gaps = 12/283 (4%)
Query: 27 IARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAY 86
+ R++ Q + + +L +LK L + RP + S+ NA++++
Sbjct: 248 LLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVG 307
Query: 87 SSGADLET---LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISV 143
SS + E LR + +E I + +AI R ++I +GDVI+ + S +
Sbjct: 308 SSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAY---QKISNGDVILVYGCSSLVSRI 364
Query: 144 MKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGA 203
++ A+ +G+ +V+V ++RP +G+ T + L G+P Y++ AA + + KV++GA
Sbjct: 365 LQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGA 424
Query: 204 DSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIP 263
++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E+ DP ++
Sbjct: 425 HALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQC 484
Query: 264 EE----ELKTWPK--NIEVWNPAFDVTPPEYIDVIITEKGIIP 300
+ L W ++ + N +DVTPPE +D++ITE G+IP
Sbjct: 485 KRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIP 527
>sp|Q9UI10|E2BD_HUMAN TRANSLATION INITIATION FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|6563202|gb|AAF17195.1|AF112207_1
(AF112207) translation initiation factor eIF-2b delta
subunit [Homo sapiens]
Length = 523
Score = 137 bits (342), Expect = 2e-31
Identities = 82/283 (28%), Positives = 150/283 (52%), Gaps = 12/283 (4%)
Query: 27 IARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAY 86
+ R++ Q + + +L +LK L + RP + S+ NA++++
Sbjct: 229 LLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVG 288
Query: 87 SSGADLET---LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISV 143
SS + E LR + +E I + +AI R ++I +GDVI+ + S +
Sbjct: 289 SSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFSY---QKISNGDVILVYGCSSLVSRI 345
Query: 144 MKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGA 203
++ A+ +G+ +V+V ++RP +G+ T + L G+P Y++ AA + + KV++GA
Sbjct: 346 LQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGA 405
Query: 204 DSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIP 263
++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E+ DP ++
Sbjct: 406 HALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQC 465
Query: 264 EE----ELKTWPKN--IEVWNPAFDVTPPEYIDVIITEKGIIP 300
+ L W + + + N +DVTPPE +D++ITE G+IP
Sbjct: 466 KRGEHVALANWQNHALLRLLNLVYDVTPPELVDLVITELGMIP 508
>pir||A55146 guanine nucleotide exchange factor eIF-2B delta chain, long form -
mouse
Length = 544
Score = 135 bits (336), Expect = 8e-31
Identities = 89/292 (30%), Positives = 154/292 (52%), Gaps = 18/292 (6%)
Query: 24 AGKIARSVAQALMIQA---EKSTAKSPE---ELWTELKEAAKILYKTRPTAVSLPNALRY 77
+G AR +A +Q + +T S E +L +LK L + RP + S+ NA+++
Sbjct: 241 SGSNARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKF 300
Query: 78 VMHRAKIAYSSGADLET---LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTH 134
+ SS + E LR + +E I + +AI R + +I DGDVI+ +
Sbjct: 301 LTKEVTGMSSSKREEEAKSELREALDRYVQEKIVLAAQAISRFA---STKISDGDVILVY 357
Query: 135 CHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMK 194
S +++ A +G+ +V+V ++RPR +G+ L G+P Y++ AA + +
Sbjct: 358 GCSSLVSRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLP 417
Query: 195 MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIE 254
KV++GA ++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E
Sbjct: 418 EVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNE 477
Query: 255 MRDPTEVI----PEEELKTWPK--NIEVWNPAFDVTPPEYIDVIITEKGIIP 300
+ DP ++ + L W ++ + N +DVTPPE +D++ITE G+IP
Sbjct: 478 LDDPDDLQCKRGDQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIP 529
>gi|6753736 eukaryotic translation initiation factor 2B [Mus musculus]
>gi|2494307|sp|Q61749|E2BD_MOUSE TRANSLATION INITIATION
FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP EXCHANGE
FACTOR) >gi|1083345|pir||B55146 guanine nucleotide
exchange factor eIF-2B delta chain, short form - mouse
>gi|529430|gb|AAA68047.1| (M98036) guanine nucleotide
exchange factor, eIF-2B, delta subunit [Mus musculus]
Length = 524
Score = 134 bits (333), Expect = 2e-30
Identities = 88/292 (30%), Positives = 154/292 (52%), Gaps = 18/292 (6%)
Query: 24 AGKIARSVAQALMIQA---EKSTAKSPE---ELWTELKEAAKILYKTRPTAVSLPNALRY 77
+G AR +A +Q + +T S E +L +LK L + RP + S+ NA+++
Sbjct: 221 SGSNARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKF 280
Query: 78 VMHRAKIAYSSGADLET---LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTH 134
+ SS + E L+ + +E I + +AI R + +I DGDVI+ +
Sbjct: 281 LTKEVTGMSSSKREEEAKSELKEALDRYVQEKIVLAAQAISRFA---STKISDGDVILVY 337
Query: 135 CHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMK 194
S +++ A +G+ +V+V ++RPR +G+ L G+P Y++ AA + +
Sbjct: 338 GCSSLVSRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLP 397
Query: 195 MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIE 254
KV++GA ++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E
Sbjct: 398 EVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNE 457
Query: 255 MRDPTEVI----PEEELKTWPK--NIEVWNPAFDVTPPEYIDVIITEKGIIP 300
+ DP ++ + L W ++ + N +DVTPPE +D++ITE G+IP
Sbjct: 458 LDDPDDLQCKRGDQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIP 509
>gb|AAA68046.1| (M98035) guanine nucleotide exchange factor, eIF-2B, delta subunit
[Mus musculus]
Length = 544
Score = 134 bits (333), Expect = 2e-30
Identities = 88/292 (30%), Positives = 154/292 (52%), Gaps = 18/292 (6%)
Query: 24 AGKIARSVAQALMIQA---EKSTAKSPE---ELWTELKEAAKILYKTRPTAVSLPNALRY 77
+G AR +A +Q + +T S E +L +LK L + RP + S+ NA+++
Sbjct: 241 SGSNARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKF 300
Query: 78 VMHRAKIAYSSGADLET---LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTH 134
+ SS + E L+ + +E I + +AI R + +I DGDVI+ +
Sbjct: 301 LTKEVTGMSSSKREEEAKSELKEALDRYVQEKIVLAAQAISRFA---STKISDGDVILVY 357
Query: 135 CHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMK 194
S +++ A +G+ +V+V ++RPR +G+ L G+P Y++ AA + +
Sbjct: 358 GCSSLVSRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLP 417
Query: 195 MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIE 254
KV++GA ++ ANG+V++++GT+ +AL A+ H V V++ ETYKF V E
Sbjct: 418 EVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNE 477
Query: 255 MRDPTEVI----PEEELKTWPK--NIEVWNPAFDVTPPEYIDVIITEKGIIP 300
+ DP ++ + L W ++ + N +DVTPPE +D++ITE G+IP
Sbjct: 478 LDDPDDLQCKRGDQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIP 529
>dbj|BAB10154.1| (AB005231) contains similarity to guanine nucleotide exchange
factor~gene_id:MBB18.19 [Arabidopsis thaliana]
Length = 642
Score = 133 bits (332), Expect = 2e-30
Identities = 90/318 (28%), Positives = 158/318 (49%), Gaps = 20/318 (6%)
Query: 9 VYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPE-----ELWTELKEAAKILYK 63
VY++ + S +I G ++ QA + K + PE ++ ++ L +
Sbjct: 321 VYKVGLQYLSGDISGGNARCIAMLQAFQ-EVVKDYSTPPEKSLNRDMTAKISSYVSFLIE 379
Query: 64 TRPTAVSLPNALRYVMHRAK---IAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEI 120
RP ++S+ NA+R+V +R I S TL+ + E I ++ I +
Sbjct: 380 CRPLSISMGNAIRFVKNRIAKLPITLSESEAKATLQSDIERFINEKIIVADTVIVKHA-- 437
Query: 121 GAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIP 180
+I DGDV++T+ A V+ A + K +V+V ++RP+ +G++ + L GI
Sbjct: 438 -VTKIRDGDVLLTYGSPTAVEMVILHAHELRKKFRVLVVDSRPKLEGQLLLRRLIKRGIN 496
Query: 181 VIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYK 240
Y +A + M KV +GA S+ +NG V +++GT+ +A+ A RV V++ E YK
Sbjct: 497 CTYTHINAISYIMHEVTKVFLGASSVLSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYK 556
Query: 241 FHPETMLGQLVEIEMRDP---TEVIPEEELK-----TWPKNIEVWNPAFDVTPPEYIDVI 292
FH L + E+ DP +EV E++ T N++ N +D TP +YI +I
Sbjct: 557 FHERVQLDSICSNELGDPNAISEVHGREDINYLDGLTNNANLQFLNLMYDATPSDYISMI 616
Query: 293 ITEKGIIPPYAAIDILKE 310
IT+ G++PP + I++E
Sbjct: 617 ITDYGMVPPTSVPVIVRE 634
>gb|AAF27156.1|AC016529_19 (AC016529) putative translation initiation factor EIF-2B alpha
subunit; 76271-74747 [Arabidopsis thaliana]
Length = 362
Score = 127 bits (317), Expect = 1e-28
Identities = 92/289 (31%), Positives = 143/289 (48%), Gaps = 16/289 (5%)
Query: 27 IARSVA--QALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNA----LRYVMH 80
+A +VA +AL S A + EL ELK+A+ L T++SL +RYV
Sbjct: 78 LAEAVAAIRALAAVIRASEASTMMELEIELKKASDTLKSWDKTSISLTAGCDLFIRYVTR 137
Query: 81 RAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAA 140
+ + Y D + + ++ A++F S KA I + I DG I+ H S+
Sbjct: 138 TSALEYE---DFNSAKSRLLERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGLSRVV 194
Query: 141 ISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVV 200
+ ++KTA K +V+ TE RP G + + EL+ IPV ++DSA + M D V
Sbjct: 195 LEILKTAAQNNKLFRVLCTEGRPDGTGVLLSSELSKLDIPVKLLLDSAVAYSMDEVDMVF 254
Query: 201 MGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTE 260
+GAD + +G +IN +GT IAL A V +AAE+YKF +L ++ +D
Sbjct: 255 VGADGVVESGGIINMMGTYQIALVAHSMNKPVYVAAESYKF------ARLYPLDQKDMAP 308
Query: 261 VI-PEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPPYAAIDIL 308
+ P E P +EV A D TPP+Y+ ++ T+ G++ P D L
Sbjct: 309 ALRPIEFGVKIPTKVEVERSARDYTPPQYLTLLFTDLGVLSPSVVSDEL 357
>gb|AAF02860.1|AC009324_9 (AC009324) Similar to initiation factor 2 subunit [Arabidopsis
thaliana] >gi|6056397|gb|AAF02861.1|AC009324_10
(AC009324) Similar to initiation factor 2 subunit
[Arabidopsis thaliana]
Length = 351
Score = 127 bits (316), Expect = 2e-28
Identities = 92/290 (31%), Positives = 144/290 (48%), Gaps = 16/290 (5%)
Query: 24 AGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNA----LRYVM 79
A +A A A +I+A ++T EL ELK+A+ L T++SL +RYV
Sbjct: 68 AEAVAAIRALAAVIRASEATTMM--ELEIELKKASDTLKSWDTTSISLTAGCDLFMRYVT 125
Query: 80 HRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKA 139
+ + + D + + V+ A++F S KA I + I DG I+ H S+
Sbjct: 126 RTSALEFE---DFNSAKSRVLERAEKFGEISCKARTIIAMLSQDFIFDGCTILVHGFSRV 182
Query: 140 AISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKV 199
++KT+ K +V+ TE RP G + A ELA IPV ++DSA + M D V
Sbjct: 183 VFEILKTSAQNKKLFRVLCTEGRPDKTGVLLANELAKLDIPVKLLIDSAVAYSMDEVDMV 242
Query: 200 VMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPT 259
+GAD + +G +IN +GT IAL A+ V +AAE+YKF +L ++ +D
Sbjct: 243 FVGADGVVESGGIINMMGTYQIALVAQSMNKPVYVAAESYKF------ARLYPLDQKDLE 296
Query: 260 EVI-PEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPPYAAIDIL 308
+ P + P +EV A D TPP+Y+ ++ T+ G++ P D L
Sbjct: 297 PALRPIDFSVPVPPKVEVERSARDYTPPQYLTLLFTDLGVLTPSVVSDEL 346
>sp|Q09924|E2BD_SCHPO PROBABLE TRANSLATION INITIATION FACTOR EIF-2B DELTA SUBUNIT (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|2130308|pir||S62591
translation initiation factor eif-2b delta subunit -
fission yeast (Schizosaccharomyces pombe)
>gi|7493512|pir||T38138 translation initiation factor
eif-2b delta subunit - fission yeast
(Schizosaccharomyces pombe) >gi|1067222|emb|CAA91965.1|
(Z67999) translation initiation factor eif-2b delta
subunit [Schizosaccharomyces pombe]
Length = 467
Score = 127 bits (315), Expect = 2e-28
Identities = 81/272 (29%), Positives = 137/272 (49%), Gaps = 11/272 (4%)
Query: 50 LWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHN 109
L T + L TRP ++S+ NA+R++ + D E +
Sbjct: 190 LTTHINSQIAYLVSTRPLSISMGNAIRFLKLEISVLDIDLTDDEGKELLLEKIDSYIRDR 249
Query: 110 SEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKI 169
A + I + ++I+DGDVI+T+ HS V+ A + GK +V+V ++RP ++G++
Sbjct: 250 IIIAGQVIVQAATEKIQDGDVILTYLHSSTVNDVLIHAKNVGKKFRVVVVDSRPEFEGRV 309
Query: 170 TAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHR 229
K L +GI YV+ SA + M+ K+ +G ++ +NGA+ ++ GTSLI+L E
Sbjct: 310 CLKLLTEHGIECTYVMISALSYIMQEVTKIFLGGHAMLSNGALYSRAGTSLISLLGHESN 369
Query: 230 VWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIP------EEE---LKTWP--KNIEVWN 278
V V+ E+YKF L LV E+ +++ EE+ L W KN+++ +
Sbjct: 370 VPVIACCESYKFTERIQLDSLVYNELAPGDQLVNMGVDDFEEKPGVLANWKSVKNLKLLS 429
Query: 279 PAFDVTPPEYIDVIITEKGIIPPYAAIDILKE 310
+DVTPP I V + E G++P + I+ E
Sbjct: 430 LKYDVTPPRLITVCVCEMGLLPSTSVPAIINE 461
>pir||T00687 hypothetical protein F6E13.20 - Arabidopsis thaliana
>gi|3212863|gb|AAC23414.1| (AC004005) putative
translation initiation factor eIF-2B delta subunit
[Arabidopsis thaliana]
Length = 307
Score = 127 bits (315), Expect = 2e-28
Identities = 80/274 (29%), Positives = 139/274 (50%), Gaps = 15/274 (5%)
Query: 49 ELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAK---IAYSSGADLETLRFTVINSAKE 105
++ ++ L + RP ++S+ NA+R+V +R I S +L + E
Sbjct: 23 DMTAQISSYVSFLIECRPLSISMGNAIRFVKNRIAKLPITLSESEAKASLHSDIERFINE 82
Query: 106 FIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRW 165
I ++ I E +I DGDV++T+ S ++ A + GK +V V ++RP+
Sbjct: 83 KIIGADTVIV---EHAVTKIRDGDVLLTYGSSTVIEMILVHAHELGKKFRVFVVDSRPKL 139
Query: 166 QGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTA 225
QGK+ + L GI Y +A + M KV +GA S+ +NG V +++GT+ +A+ A
Sbjct: 140 QGKLLLRRLIKRGINCTYTHITAISYIMHQVTKVFLGASSVFSNGTVYSRVGTACVAMVA 199
Query: 226 KEHRVWVMIAAETYKFHPETMLG-QLVEIEMRDP---TEVIPEEELK-----TWPKNIEV 276
RV V++ E YKFH + L + E+ DP ++V E++ T ++
Sbjct: 200 NAFRVPVLVCCEAYKFHEKVQLDYSICSNELGDPNAISKVHGREDINYLNGLTNNAKLKF 259
Query: 277 WNPAFDVTPPEYIDVIITEKGIIPPYAAIDILKE 310
N +D TP +YI +IIT+ G++PP + I++E
Sbjct: 260 LNLMYDATPSDYISMIITDYGMVPPTSVPVIVRE 293
>gb|AAC95160.1| (AC005970) putative translation initiation factor eIF-2B alpha
subunit [Arabidopsis thaliana]
Length = 365
Score = 122 bits (303), Expect = 6e-27
Identities = 95/313 (30%), Positives = 150/313 (47%), Gaps = 22/313 (7%)
Query: 6 VREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTA--KSPEELWTELKEAAKILYK 63
+R+ + I+ M +RGA IA + A +L ++ S + L+ L
Sbjct: 36 IRDASDGWSAIQEMVVRGAPAIAIAAALSLAVEVFNFHGFDGSASDAVAFLENKLDYLVS 95
Query: 64 TRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAK 123
+RPTAV+L +A + H A ++ + +++ I ++++ + + + + IG G
Sbjct: 96 SRPTAVNLADAALKLKHVIAKALATATEAKSIFKAYIEASEDMLEDDVVSNKAIGNFGLS 155
Query: 124 RIE------DGDVIMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-K 168
+ D ++THC++ + A+ V++ QG + TETRP QG +
Sbjct: 156 LLRQQAKNPDKLSVLTHCNTGSLATAGYGTALGVIRALHTQGILERAYCTETRPFNQGSR 215
Query: 169 ITAKELASYGIPVIYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAK 226
+TA EL IP + DSAA MK D V++GAD + +NG NKIGT +AL AK
Sbjct: 216 LTAFELVHEKIPATLIADSAAAALMKDGRVDGVIVGADRVASNGDTANKIGTYSLALCAK 275
Query: 227 EHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEE---ELKTWPKNIEVWNPAFDV 283
H + +AA G+ + IE R P E++ + I VWNPAFD+
Sbjct: 276 HHGIPFYVAAPLTSVDLSLSSGKEIVIEERSPKELMHTHGGLGERIAAPGISVWNPAFDM 335
Query: 284 TPPEYIDVIITEK 296
TP E I IITEK
Sbjct: 336 TPAELIAGIITEK 348
>gb|AAF57154.1| (AE003778) CG11334 gene product [alt 1] [Drosophila melanogaster]
>gi|7302053|gb|AAF57155.1| (AE003778) CG11334 gene
product [alt 2] [Drosophila melanogaster]
Length = 364
Score = 120 bits (299), Expect = 2e-26
Identities = 99/324 (30%), Positives = 148/324 (45%), Gaps = 26/324 (8%)
Query: 4 MIVREVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYK 63
++VR V + + I M++RGA IA +L ++ ++ + L E++ L
Sbjct: 28 VVVRGVEDGWKVINKMQVRGAPAIAIVGCLSLAVEINPEDFENKKSLRQEIEGKLNYLVS 87
Query: 64 TRPTAVSLPNALRYVMHRAKIAYSSGA-DLETLRFTVINSAKEFIHNSEKAIERIGEIGA 122
RPTAV++ A ++ A Y A D+ ++ +++ + + IG GA
Sbjct: 88 ARPTAVNMKIAADELITLANELYKDEAIDVTQMKHRFLDATEAMLKKDIADNRAIGANGA 147
Query: 123 KRIEDGDV--------------IMTHCHSKA--------AISVMKTAFDQGKDIKVIVTE 160
+ I G ++THC++ + A+ V++ + GK V TE
Sbjct: 148 QAILQGVAKAGKAPAGSTGSVRVLTHCNTGSLATAGYGTALGVVRQLAELGKLEHVYCTE 207
Query: 161 TRPRWQG-KITAKELASYGIPVIYVVDS--AARHYMKMTDKVVMGADSITANGAVINKIG 217
TRP QG ++TA EL P V+DS AA K VV+GAD + +NG NKIG
Sbjct: 208 TRPYNQGARLTAYELVHEKFPATLVLDSMVAALLRAKNVAAVVVGADRVASNGDTANKIG 267
Query: 218 TSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVW 277
T IA+ AK H V +AA G + IE R E+ E + I W
Sbjct: 268 TYQIAVVAKHHDVPFYVAAPLTSIDLAIPGGDHIIIEERPDREMTHVGEHRIAAPGINCW 327
Query: 278 NPAFDVTPPEYIDVIITEKGIIPP 301
NPAFDVTP I IITE+G+ P
Sbjct: 328 NPAFDVTPASLITGIITERGVFKP 351
>gb|AAF46995.1| (AE003460) CG10315 gene product [Drosophila melanogaster]
Length = 626
Score = 118 bits (294), Expect = 6e-26
Identities = 80/277 (28%), Positives = 140/277 (49%), Gaps = 9/277 (3%)
Query: 29 RSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAYSS 88
R V A+K +S L +K L+K RP AVS+ NA + ++ +
Sbjct: 338 RQVVHDFETPAKKEFGRS---LDAAVKHHVDHLHKCRPLAVSVSNAYKQFKNQLTQLPAD 394
Query: 89 GADLETLRFTVINSAKEFIHNS-EKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTA 147
+ E+ +++ +I N KA + I ++I DGDV++T S + + A
Sbjct: 395 VPETESKEL-LVHFIDTYIENQIGKAAQAISGFLQEKITDGDVLLTFACSSLIQFICEEA 453
Query: 148 FDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSIT 207
+ +VIV ++RP +G+ + L + GIP YV+ +A + M KV++GA ++
Sbjct: 454 KRRQVAFRVIVVDSRPGCEGQELLRRLHATGIPCTYVLINAVGYVMAEATKVLLGAHALL 513
Query: 208 ANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEE-- 265
ANG V+ + GT+ +AL A H V V++ ET+KF +V E+ DP +++ +
Sbjct: 514 ANGYVMARTGTAQVALVANAHNVPVLVCCETHKFSERFQTDAIVYNELSDPNQLVRGDKC 573
Query: 266 ELKTWPKNIEV--WNPAFDVTPPEYIDVIITEKGIIP 300
+L W ++ N ++D+TPPE + ++TE I+P
Sbjct: 574 QLSNWAAKGKLLPLNLSYDITPPELVSAVVTEVAILP 610
>emb|CAB90925.1| (AL355774) putative translation initiation factor [Streptomyces
coelicolor A3(2)]
Length = 380
Score = 117 bits (290), Expect = 2e-25
Identities = 89/317 (28%), Positives = 150/317 (47%), Gaps = 36/317 (11%)
Query: 12 IAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSL 71
+ E I+S+ +RGA + + + + A + E++EAA L RPTAV+L
Sbjct: 54 LVEAIRSLAVRGAPLLGIAGGYGVALAAVRGF---------EVEEAAAALAGARPTAVNL 104
Query: 72 PNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIED---- 127
+R + A + D + +A+ +A R+ G +++
Sbjct: 105 AVGVRRAQAAHREALAGTGDTRQAARAALAAARALHREDTEASARMAAHGLALLDELLPA 164
Query: 128 -GDVIMTHCHSKAAIS--------VMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASY 177
G ++THC++ + +S V A G+ ++ V ETRP QG ++TA E A
Sbjct: 165 GGHRVLTHCNTGSLVSGGEGTAFAVALAAHRSGRLRRLWVDETRPLLQGARLTAYEAARN 224
Query: 178 GIPVIYVVDSAARHYMKM--TDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIA 235
+ + D+AA D V++GAD I A+G+V NK+G+ +A+ A+ H V ++
Sbjct: 225 DMAYTLLTDNAAGSLFAAGEVDAVLIGADRIAADGSVANKVGSYPLAVLARYHHVPFVVV 284
Query: 236 AETYKFHPETMLGQLVEIEMR---DPTEVIPEE--------ELKTWPKNIEVWNPAFDVT 284
A P+T G +E+E R + TEV + + P + +NPAFDVT
Sbjct: 285 APVTTVDPDTPDGASIEVEQRPGYEVTEVTAPQVPVAGAGGGIPVAPLGTQAYNPAFDVT 344
Query: 285 PPEYIDVIITEKGIIPP 301
PPE + I+TE+G++ P
Sbjct: 345 PPELVTAIVTEEGVVSP 361
>pir||T40858 translation initiation factor eif-2b alpha subunit - fission yeast
(Schizosaccharomyces pombe) >gi|6016995|emb|CAB57849.1|
(AL121783) translation initiation factor eif-2b alpha
subunit [Schizosaccharomyces pombe]
Length = 341
Score = 114 bits (283), Expect = 1e-24
Identities = 85/286 (29%), Positives = 130/286 (44%), Gaps = 19/286 (6%)
Query: 33 QALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAYSSGADL 92
+AL+ +S AK+ E L+ + L + +SL + D
Sbjct: 41 EALVQLLSRSQAKTISEFMDILQNGSNTLKEGVQNNISLSAGCDIFQRFVTRSLHDVGDF 100
Query: 93 ETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGK 152
E + ++ + K FI + +RI +G I DG VI+TH S+ +V+ A +
Sbjct: 101 EQCKRHLVENGKLFIQRARACRQRIAHLGYPLIRDGSVILTHGFSRGVAAVLLAAAKRHV 160
Query: 153 DIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAV 212
KV VTE+RP G + + L + IP V+DSA M D V++GA+ + NG +
Sbjct: 161 RFKVFVTESRPSGSGCLMTRTLKNACIPTCMVLDSAVSFTMNRVDLVLVGAEGVVENGGL 220
Query: 213 INKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQ--------LVEIEMRDPTEVIPE 264
IN+IGT +A+ AK AE++KF L Q ++E + P V PE
Sbjct: 221 INQIGTFQLAVFAKHAHKPFYAVAESHKFVRMFPLSQYDIPFSRPILEFDDPSPETVHPE 280
Query: 265 EELKTWPKNI---EVW--------NPAFDVTPPEYIDVIITEKGII 299
E P + E+ NP DVTPPE++ +IT+ GII
Sbjct: 281 PEPIPTPSDAIHNELIMNEEQIRNNPTLDVTPPEFVSGLITDLGII 326
>gb|AAF69705.1|AC016041_10 (AC016041) F27J15.24 [Arabidopsis thaliana]
>gi|11094807|gb|AAG29736.1|AC084414_4 (AC084414) guanine
nucleotide exchange factor eIF-2B delta subunit,
putative [Arabidopsis thaliana]
Length = 756
Score = 113 bits (279), Expect = 4e-24
Identities = 84/308 (27%), Positives = 156/308 (50%), Gaps = 23/308 (7%)
Query: 9 VYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKI------LY 62
VY++ + + +I G ++ QA E + ++L +L AKI L
Sbjct: 429 VYKVGLQHLAGDISGDNARCIAMLQAFQEAIEDYSTPPMKDLTMDL--TAKINGYVSFLI 486
Query: 63 KTRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAI---ERIGE 119
+ RP ++S+ NA+R++ ++ + + ++ E + ++ + FI EK I + I +
Sbjct: 487 ECRPLSMSMGNAIRFLKNQIRKLPVNLSESEA-KSSLCSDIGRFI--DEKTIIADKAIVQ 543
Query: 120 IGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGI 179
+I DG+V++T+ S ++ A + GK +V++ ++RP +G+ + L + G+
Sbjct: 544 HAVTKIRDGEVLLTYGFSCVVEMILLYAHEIGKKFRVVIVDSRPNLEGQKLLRRLVTRGL 603
Query: 180 PVIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETY 239
Y +A + M+ +V +GA SI +NG + ++GTS IA+ A V V++ E Y
Sbjct: 604 DCTYTHINAISYIMREATRVFLGASSIYSNGTLYARVGTSCIAMVANAFSVPVIVCCEAY 663
Query: 240 KFHPETMLGQLVEIEMRDPTEV--IPE-----EELKTWP--KNIEVWNPAFDVTPPEYID 290
KFH +L + E+ DP V IP + KT KN++ N +D TP EYI
Sbjct: 664 KFHERVLLDSICSNELGDPDAVANIPSFRTNAKHSKTMDNNKNLQFLNLMYDSTPSEYIS 723
Query: 291 VIITEKGI 298
+I+++ G+
Sbjct: 724 MIVSDYGM 731
>sp|P34604|E2BA_CAEEL PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B ALPHA SUBUNIT (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|482164|pir||S40927
hypothetical protein ZK1098.4 - Caenorhabditis elegans
>gi|3881483|emb|CAA80132.1| (Z22176) similar to GCN3
(yeast transcription activator), contains similarity to
Pfam domain: PF01008 (Initiation factor 2 subunit
family), Score=413.9, E-value=5e-121, N=1
[Caenorhabditis elegans]
Length = 305
Score = 112 bits (278), Expect = 5e-24
Identities = 80/278 (28%), Positives = 130/278 (45%), Gaps = 11/278 (3%)
Query: 30 SVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAYSSG 89
+ + L+ ++S A + E EL + L KT ++ S+ +A +A ++
Sbjct: 25 ATVETLLEVLDRSRATTVAEFQNELNQVVAALEKTDYSSTSIRSAADLFTRFTSLAPAAL 84
Query: 90 ADLETLRFTVI---NSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKT 146
D E + A+ FI N + +I + I+TH +SK + +
Sbjct: 85 LDQEDFSQVLDLYRQRARSFIKNVRGSRAKISKCARLFFTHHMNILTHSYSKVVLETILD 144
Query: 147 AFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSI 206
A G + V VTE++P GK+ +EL G+P V+DS + M+ V++GA+ +
Sbjct: 145 AHKSGYHLHVWVTESQPDASGKLVFEELKKNGVPTTLVLDSCVGYVMERIQAVLVGAEGV 204
Query: 207 TANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQL-VEIEMRDPTEVIPEE 265
G +INKIGT + + AK V V + AET KF E L Q + E + T VI
Sbjct: 205 METGGIINKIGTVNVCIIAKSRHVPVYVCAETIKFVREFPLNQADIPQEFKYRTSVIER- 263
Query: 266 ELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPPYA 303
N+E+ +P D T PE++ +IIT+ G + P A
Sbjct: 264 ------NNLELEHPDVDYTAPEFLTLIITDVGAMKPEA 295
>gi|6322878 34 KD alpha subunit of eIF2B; Gcn3p [Saccharomyces cerevisiae]
>gi|121065|sp|P14741|E2BA_YEAST TRANSLATION INITIATION
FACTOR EIF-2B ALPHA SUBUNIT (EIF-2B GDP-GTP EXCHANGE
FACTOR) (GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT
GCN3) (GCD COMPLEX SUBUNIT GCN3) (TRANSCRIPTIONAL
ACTIVATOR GCN3) >gi|83571|pir||A31562 guanine nucleotide
exchange factor eIF-2B delta chain [validated] - yeast
(Saccharomyces cerevisiae) >gi|171580|gb|AAA34637.1|
(M23356) transcriptional activator protein GCN3
[Saccharomyces cerevisiae] >gi|486453|emb|CAA82098.1|
(Z28251) ORF YKR026c [Saccharomyces cerevisiae]
Length = 305
Score = 111 bits (274), Expect = 1e-23
Identities = 77/282 (27%), Positives = 136/282 (47%), Gaps = 16/282 (5%)
Query: 33 QALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNA----LRYVMHRAKIAYSS 88
+AL+ T ++ E+ +K + + L K+ P +VSL +R+V+ +
Sbjct: 28 EALVTLLRIKTPETAAEMINTIKSSTEELIKSIPNSVSLRAGCDIFMRFVLRNLHLY--- 84
Query: 89 GADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAF 148
D E + +I + + F+ ++K+ +I EIG I D D+I+ H +S+A S++ A
Sbjct: 85 -GDWENCKQHLIENGQLFVSRAKKSRNKIAEIGVDFIADDDIILVHGYSRAVFSLLNHAA 143
Query: 149 DQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITA 208
++ + +VTE+RP QG L GIPV +VDSA + DKV +GA+ +
Sbjct: 144 NKFIRFRCVVTESRPSKQGNQLYTLLEQKGIPVTLIVDSAVGAVIDKVDKVFVGAEGVAE 203
Query: 209 NGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELK 268
+G +IN +GT + + A R + E++KF ++ + D P +
Sbjct: 204 SGGIINLVGTYSVGVLAHNARKPFYVVTESHKF------VRMFPLSSDDLPMAGPPLDFT 257
Query: 269 TWPKNIE--VWNPAFDVTPPEYIDVIITEKGIIPPYAAIDIL 308
++E + P D T EYI +IT+ G++ P A + L
Sbjct: 258 RRTDDLEDALRGPTIDYTAQEYITALITDLGVLTPSAVSEEL 299
>sp|Q93169|YQJQ_CAEEL HYPOTHETICAL 40.9 KD PROTEIN C01G10.9 IN CHROMOSOME V
>gi|7495123|pir||T18820 hypothetical protein C01G10.9 -
Caenorhabditis elegans >gi|3873873|emb|CAB02711.1|
(Z81030) Strong similarity with yeast hypothetical
protein (Swiss Prot accession number P23140), contains
similarity to Pfam domain: PF01008 (Initiation factor 2
subunit family), Score=490.1, E-value=5.7e-144, N=1~cDNA
EST yk77c8.3 comes from this gene>
Length = 366
Score = 108 bits (268), Expect = 7e-23
Identities = 90/305 (29%), Positives = 143/305 (46%), Gaps = 28/305 (9%)
Query: 16 IKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNAL 75
IKSM++RGA IA + L+++ +K++ E + ++ L +RPTAV L N+L
Sbjct: 62 IKSMQVRGAPLIAVVGSLGLLLEIQKASELDSESIIQKIN----FLISSRPTAVDLRNSL 117
Query: 76 RYVMHRAKIA-YSSGADLETLRFTVINSAKEFIHNSEKAIERI-------GEIGAKRIED 127
+ + YS LE R ++N ++ EK RI + A ++
Sbjct: 118 NGLKPILESQDYSDVVKLEKCRSYLLN-----VYTDEKLQNRILVWNAYQELLSAFPDKE 172
Query: 128 GDVIMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASYG 178
+MT C++ + A+ V++ + + V V ETRP QG ++T+ EL
Sbjct: 173 KLTVMTICNTGSLATISWGTALGVIRALHSENRLKLVYVLETRPYNQGIRLTSIELLHGE 232
Query: 179 IPVIYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAA 236
+P + DS A MK D V+ GAD++ NG NKIGT ++A+ K H +
Sbjct: 233 VPFKLITDSMAAWAMKNHQVDCVLTGADNVARNGDTANKIGTYMLAVLCKHHNINFYPVV 292
Query: 237 ETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEK 296
+ G+ ++IE R E++ + VWNPAFDVTP I I+T+
Sbjct: 293 PFTTINKNIATGEEIKIEERPSAEMLRVNGVLIGNSECPVWNPAFDVTPAHLITKILTDF 352
Query: 297 GIIPP 301
G PP
Sbjct: 353 GNWPP 357
>gb|AAF20216.1|AC012395_3 (AC012395) putative translation initiation factor EIF-2B beta
subunit [Arabidopsis thaliana]
Length = 407
Score = 108 bits (266), Expect = 1e-22
Identities = 73/253 (28%), Positives = 120/253 (46%), Gaps = 30/253 (11%)
Query: 70 SLPNALRYVMHRAKIAY--SSGADLET-----------LRFTVINSAKEFIHNSEKAIER 116
SL L A + Y SSGAD E+ L+ VI + I E+
Sbjct: 138 SLQTLLEGTPESATVPYTSSSGADSESKTADKSSLTRKLKHDVIEGVNQLIQEIAGCHEQ 197
Query: 117 IGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELAS 176
I E + I +VI+T S+ + + A ++ + +V V E PR+QG + AKEL +
Sbjct: 198 IAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPRYQGHLLAKELVA 257
Query: 177 YGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAA 236
G+ + DSA + + V++GA ++ ANG VI +G ++ AL A++H V ++ A
Sbjct: 258 RGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGVIGPVGVNMAALAAQKHAVPFVVLA 317
Query: 237 ETYKF------HPETMLGQLVEIEMRDPTEVIPEEELK------TWPKNIEVWNPAFDVT 284
++K +PE +L E+R P+E++ E +++V NP FD
Sbjct: 318 GSHKLCPLYPHNPEVLLN-----ELRSPSELLDFGEFSDCLDFGAGSGSLQVVNPTFDYV 372
Query: 285 PPEYIDVIITEKG 297
PP + + IT+ G
Sbjct: 373 PPNLVSLFITDTG 385
>pir||T39944 hypothetical translation initiation factor - fission yeast
(Schizosaccharomyces pombe) >gi|3116128|emb|CAA18878.1|
(AL023287) translation initiation factor eif-2 family
[Schizosaccharomyces pombe]
Length = 359
Score = 107 bits (264), Expect = 2e-22
Identities = 99/329 (30%), Positives = 147/329 (44%), Gaps = 49/329 (14%)
Query: 6 VREVYEIAEKIKSMEIRGAGKIA----RSVAQALMIQAEKSTAKSPEELWTELKEAAKIL 61
V V + IK M++RGA IA SVA L +E + AK + + + L
Sbjct: 30 VSNVEDAFAVIKKMQVRGAPAIAIVAALSVAVGLKHLSEDADAKE------YVINSFEHL 83
Query: 62 YKTRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIG 121
++RPTAV+L +++ IA G + R +I A+ + + IG G
Sbjct: 84 KQSRPTAVNLFETAKFMQG---IALQEG---KNCRNKIIQEAERMLVKDLEDNHNIGTAG 137
Query: 122 AKRI------EDGDVIMTHCHSKA--------AISVMKTAFDQGKDIKVIVTETRPRWQG 167
K + +D ++THC++ + A+ ++++ + G TETRP QG
Sbjct: 138 RKFLLQHYKDKDKLTVLTHCNTGSLATSGYGTALGIIRSLHESGNLEHAYCTETRPYNQG 197
Query: 168 -KITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAK 226
++TA EL IP V DS M D +V+GAD +T NG NKIGT +A+ AK
Sbjct: 198 SRLTAFELVHDKIPATLVTDSTVASIMHKIDAIVVGADRVTRNGDTANKIGTYNLAILAK 257
Query: 227 EHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTE-------VIPEEELKTW--PK----- 272
++AA G + IE R P E +I + + PK
Sbjct: 258 HFGKPFIVAAPFSSIDTSLASGSQITIEQRPPIEMTTITGPIITDSNSRNLEDPKRVRIS 317
Query: 273 ----NIEVWNPAFDVTPPEYIDVIITEKG 297
I+V++PAFDVTP I I TEKG
Sbjct: 318 IAAPGIDVYSPAFDVTPANLITAIATEKG 346
>gb|AAD52847.2|AF137288_1 (AF137288) putative translation initiation factor 2B beta subunit
[Nicotiana tabacum]
Length = 415
Score = 106 bits (261), Expect = 5e-22
Identities = 68/229 (29%), Positives = 112/229 (48%), Gaps = 23/229 (10%)
Query: 87 SSGADLET----LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAIS 142
S AD T L+ +I + E I + E+I E + I +VI+T +S+ +
Sbjct: 164 SKSADKNTASRKLKHNIIEAVNELIQDIATCHEQIAEQAVEHIHHNEVILTLGNSRTVME 223
Query: 143 VMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMG 202
+ A ++ + +V V E PR+QG AKEL + G+ + DSA + + VV+G
Sbjct: 224 FLCAAKEKKRSFRVFVAEGAPRYQGHALAKELVARGLQTTVITDSAIFAMISRVNMVVVG 283
Query: 203 ADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKF------HPETMLGQLVEIEMR 256
A ++ ANG VI +G +++AL A+ H V ++ A T+K +PE +L E++
Sbjct: 284 AHAVMANGGVIAPVGMNMVALAAQRHAVPFVVLAGTHKLCPLYPHNPEVLLN-----ELK 338
Query: 257 DPTEVIPEEEL--------KTWPKNIEVWNPAFDVTPPEYIDVIITEKG 297
P E++ E + + V NPAFD PP + + IT+ G
Sbjct: 339 SPAELLDFGEFSDCLDFGSSSGSPLLHVVNPAFDYVPPNLVSLFITDTG 387
>gi|4503503 eukaryotic translation initiation factor 2B, subunit 1 (alpha,
26kD); eukaryotic translation initiation factor 2B;
eIF-2-beta [Homo sapiens] >gi|11433185|ref|XP_006566.1|
eukaryotic translation initiation factor 2B, subunit 1
(alpha, 26kD) [Homo sapiens]
>gi|2494303|sp|Q14232|E2BA_HUMAN TRANSLATION INITIATION
FACTOR EIF-2B ALPHA SUBUNIT (EIF-2B GDP-GTP EXCHANGE
FACTOR) >gi|1200232|emb|CAA64950.1| (X95648) alfa
subunit [Homo sapiens]
Length = 305
Score = 104 bits (256), Expect = 2e-21
Identities = 66/228 (28%), Positives = 117/228 (50%), Gaps = 7/228 (3%)
Query: 82 AKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAI 141
A + YS D + +I + F+ + +I ++ I+DG I+TH +S+ +
Sbjct: 79 ASLEYS---DYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGATILTHAYSRVVL 135
Query: 142 SVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVM 201
V++ A K V VTE++P GK AK L +PV V+D+A + M+ D V++
Sbjct: 136 RVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKADLVIV 195
Query: 202 GADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEV 261
GA+ + NG +INKIGT+ +A+ AK + AE++KF L L + ++ D +
Sbjct: 196 GAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKF---VRLFPLNQQDVPDKFKY 252
Query: 262 IPEE-ELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPPYAAIDIL 308
+ ++ ++++ +P D T P I ++ T+ G++ P A D L
Sbjct: 253 KADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDEL 300
>sp|Q64270|E2BA_RAT TRANSLATION INITIATION FACTOR EIF-2B ALPHA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|2144003|pir||I59376 translation
initiation factor eIF-2B alpha chain [validated] - rat
>gi|623033|gb|AAC52196.1| (U05821) translation
initiation factor eIF-2B alpha-subunit [Rattus
norvegicus] >gi|1050328|gb|AAA91276.1| (L41679)
GTP-exchange protein [Rattus norvegicus]
>gi|1096885|prf||2112359A initiation factor eIF-2B
[Rattus norvegicus]
Length = 305
Score = 101 bits (250), Expect = 9e-21
Identities = 78/302 (25%), Positives = 150/302 (48%), Gaps = 10/302 (3%)
Query: 11 EIAEKIKSMEIRGAGKIARSVA--QALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTA 68
E+ + KS +++G +A +VA Q L+ ++ ++ + L L +A + L ++
Sbjct: 5 ELIKYFKS-QMKGDPNMASAVAAIQTLLEFLKRDKGETIQGLRAHLTKAIETLCAV-DSS 62
Query: 69 VSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDG 128
V++ + + + +D + +I + F+ + +I + I+DG
Sbjct: 63 VAVSSGGELFLRFISLTSLEYSDYSKCKKIMIERGELFLSRISLSRTKIASLCHAFIKDG 122
Query: 129 DVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSA 188
I+TH +S+ + V++ A K V +TE++P GK AK L +PV V+D+A
Sbjct: 123 ARILTHAYSRVVLRVLEEAVAAKKRFSVYITESQPDLSGKKMAKALCHLNVPVTVVLDAA 182
Query: 189 ARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLG 248
+ M+ D V++GA+ + NG +INKIGT+ +A+ AK + AE++KF L
Sbjct: 183 VGYIMEKVDLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKF---VRLF 239
Query: 249 QLVEIEMRDPTEVIPEEELKTWP--KNIEVWNPAFDVTPPEYIDVIITEKGIIPPYAAID 306
L + ++ D + + LK+ ++++ +P D T P I ++ T+ G++ P A D
Sbjct: 240 PLNQQDVPDKFK-YKADTLKSVQAGQDLKEEHPWVDYTSPSLITLLFTDLGVLTPSAVSD 298
Query: 307 IL 308
L
Sbjct: 299 EL 300
>gb|AAG19758.1| (AE005061) translation initiation factor eIF-2B subunit delta;
Eif2bd [Halobacterium sp. NRC-1]
Length = 288
Score = 97.1 bits (238), Expect = 2e-19
Identities = 78/290 (26%), Positives = 127/290 (42%), Gaps = 17/290 (5%)
Query: 11 EIAEKIKSMEIRGAGKIARSVAQAL--MIQAEKSTAKSPEELWTELKEAAKILYKTRPTA 68
E AE+I+ M + +A A+AL ++ ++ T + L+ + L + P+
Sbjct: 4 ETAEEIREMRTHSSSVVAVKAARALEALLDSDYPTVAAYVRA---LERNSTALRRANPSH 60
Query: 69 VSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDG 128
SL R V+ R + A A + + + + + A+ I DG
Sbjct: 61 ASLVTTQREVVSRVRDA--DPASIPEAQEVTAAAIDDVVARVNMGKHDAAAATAEHITDG 118
Query: 129 DVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELASYG-IPVIYVVDS 187
++TH +S + ++ A G + V VTE RPR G+ T + LA + +VD
Sbjct: 119 QTLLTHDYSSTVLEAIELAAQDGAHLDVFVTEARPRHLGRKTVRALAQLDRVDPTLIVDG 178
Query: 188 AARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETML 247
AA H + D+V++G D I A+ N++GT I A + V +A K +
Sbjct: 179 AAGHVLDDCDRVLLGMDCIVAD-TYYNRVGTYPIVAAAADAGTPVTVAGSAAKLVAD--- 234
Query: 248 GQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKG 297
G + RD +EVI E P V NPA+D TP +D ++TE G
Sbjct: 235 GFQFTNDFRDTSEVIREP-----PAGFTVENPAYDATPTRLLDAVVTEDG 279
>gi|6325375 Ypr118wp [Saccharomyces cerevisiae]
>gi|3183489|sp|Q06489|YP18_YEAST HYPOTHETICAL 45.0 KD
PROTEIN IN PIS1-CLB2 INTERGENIC REGION
>gi|2133029|pir||S69011 translation initiation factor
eIF-2B alpha chain homolog YPR118w - yeast
(Saccharomyces cerevisiae) >gi|1066474|gb|AAB68059.1|
(U40828) P9642.7 gene product [Saccharomyces cerevisiae]
Length = 411
Score = 96.7 bits (237), Expect = 3e-19
Identities = 100/356 (28%), Positives = 155/356 (43%), Gaps = 77/356 (21%)
Query: 16 IKSMEIRGAGKIA--RSVAQALMIQAEKSTAKSP----------EELWTELKEAAKILYK 63
IKSM++RGA IA S++ +Q K S E T L + L
Sbjct: 45 IKSMQVRGAPAIAIVGSLSVLTEVQLIKHNPTSDVATLYSLVNWESTKTVLNKRLDFLLS 104
Query: 64 TRPTAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAK 123
+RPTAV+L N+L + + K S +DL+ ++ N E I ++G+ GAK
Sbjct: 105 SRPTAVNLSNSLVEIKNILK----SSSDLKAFDGSLYNYVCELIDEDLANNMKMGDNGAK 160
Query: 124 RI---------EDGDVIMTHCHSKA--------AISVMKTAF----------DQGKDIK- 155
+ +D ++T C++ + A+ V+++ + D G D +
Sbjct: 161 YLIDVLQKDGFKDEFAVLTICNTGSLATSGYGTALGVIRSLWKDSLAKTDKADSGLDNEK 220
Query: 156 ------VIVTETRPRWQG-KITAKELASYGIPVIYVVDSAARHYMKMTDKVV----MGAD 204
V ETRP QG ++TA EL IP + DS+ + ++ + + +GAD
Sbjct: 221 CPRMGHVFPLETRPYNQGSRLTAYELVYDKIPSTLITDSSIAYRIRTSPIPIKAAFVGAD 280
Query: 205 SITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTE---- 260
I NG NKIGT +A+ K+ + + A T G + +E R+P E
Sbjct: 281 RIVRNGDTANKIGTLQLAVICKQFGIKFFVVAPKTTIDNVTETGDDIIVEERNPEEFKVV 340
Query: 261 ----VIPEE--------------ELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGI 298
+ PE ++ P I VWNPAFD+TP E ID IITE+G+
Sbjct: 341 TGTVINPENGSLILNESGEPITGKVGIAPLEINVWNPAFDITPHELIDGIITEEGV 396
>gb|AAF56977.1| (AE003771) CG7883 gene product [Drosophila melanogaster]
Length = 306
Score = 95.6 bits (234), Expect = 7e-19
Identities = 72/277 (25%), Positives = 130/277 (45%), Gaps = 11/277 (3%)
Query: 33 QALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAYSSGADL 92
+ L++ EK + L T ++EA + T + ++ +A ++ +
Sbjct: 35 RTLLMILEKKQFGTIHILHTTMREAVAAMRNTDLSIAAIVSAGELFCRFITLSLDD-KHM 93
Query: 93 ETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGK 152
E R ++N K F+ + + I + + I DG I+TH S+ + + TA K
Sbjct: 94 EECRQIMLNRGKIFLTKLLNSRQVIAQQAQRFITDGCRILTHSRSRVVLKALITASQNKK 153
Query: 153 DIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAV 212
V VT+ G+ K+L + GI ++DSA + M+ D V++GA+++ +G +
Sbjct: 154 SFHVYVTQGGTGNSGEEMVKDLHAAGIDCTLILDSATGYVMESVDFVLVGAEAVVESGGI 213
Query: 213 INKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRD-PTEVIPEEELKTWP 271
IN+IGT + L A+E + + AE++KF +L + RD P E + +
Sbjct: 214 INRIGTYTMGLCAREMKKPFYVLAESFKF------SRLYPLNQRDLPNEY---KYSRKHL 264
Query: 272 KNIEVWNPAFDVTPPEYIDVIITEKGIIPPYAAIDIL 308
++ +P D TPP YI ++ T+ G + P A D L
Sbjct: 265 NDVSKVHPLVDYTPPVYITLLFTDLGRLTPSAVSDEL 301
>gb|AAG38014.1| (AF289100) eukaryotic initiation factor eIF2B alpha subunit
[Drosophila melanogaster]
Length = 300
Score = 95.6 bits (234), Expect = 7e-19
Identities = 72/277 (25%), Positives = 130/277 (45%), Gaps = 11/277 (3%)
Query: 33 QALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMHRAKIAYSSGADL 92
+ L++ EK + L T ++EA + T + ++ +A ++ +
Sbjct: 29 RTLLMILEKKQFGTIHILHTTMREAVAAMRNTDLSIAAIVSAGELFCRFITLSLDD-KHM 87
Query: 93 ETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGK 152
E R ++N K F+ + + I + + I DG I+TH S+ + + TA K
Sbjct: 88 EECRQIMLNRGKIFLTKLLNSRQVIAQQAQRFITDGCRILTHSRSRVVLKALITASQNKK 147
Query: 153 DIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAV 212
V VT+ G+ K+L + GI ++DSA + M+ D V++GA+++ +G +
Sbjct: 148 SFHVYVTQGGTGNSGEEMVKDLHAAGIDCTLILDSATGYVMESVDFVLVGAEAVVESGGI 207
Query: 213 INKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRD-PTEVIPEEELKTWP 271
IN+IGT + L A+E + + AE++KF +L + RD P E + +
Sbjct: 208 INRIGTYTMGLCAREMKKPFYVLAESFKF------SRLYPLNQRDLPNEY---KYSRKHL 258
Query: 272 KNIEVWNPAFDVTPPEYIDVIITEKGIIPPYAAIDIL 308
++ +P D TPP YI ++ T+ G + P A D L
Sbjct: 259 NDVSKVHPLVDYTPPVYITLLFTDLGRLTPSAVSDEL 295
>gi|6321520 translation initiation factor eIF2B, 71 kDa (delta) subunit;
translational repressor of GCN4 protein; Gcd2p
[Saccharomyces cerevisiae]
>gi|121058|sp|P12754|E2BD_YEAST TRANSLATION INITIATION
FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP EXCHANGE
FACTOR) (GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT
GCD2) (GCD COMPLEX SUBUNIT GCD2) >gi|73255|pir||RGBYD2
translation regulator GCD2 - yeast (Saccharomyces
cerevisiae) >gi|3734|emb|CAA33693.1| (X15658) GCD2 gene
product (AA 1 - 651) [Saccharomyces cerevisiae]
>gi|1323119|emb|CAA97085.1| (Z72868) ORF YGR083c
[Saccharomyces cerevisiae]
Length = 651
Score = 93.2 bits (228), Expect = 4e-18
Identities = 69/249 (27%), Positives = 126/249 (49%), Gaps = 14/249 (5%)
Query: 25 GKIARSVAQALMIQAEKSTAKSPE------ELWTELKEAAKILYKTRPTAVSLPNALRYV 78
G I R +A + Q ++P+ L + L +L K RP +V++ NA+R++
Sbjct: 288 GSIPRCIAMLEVFQIVIKDYQTPKGTTLSRNLTSYLSHQIDLLKKARPLSVTMGNAIRWL 347
Query: 79 MHRAKIAYSSGADL---ETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHC 135
+ S D + L + AKE I E A + I + + +IE+ I+T+
Sbjct: 348 KQEISLIDPSTPDKAAKKDLCEKIGQFAKEKI---ELADQLIIDNASTQIEESTTIVTYG 404
Query: 136 HSKAAISVM-KTAFDQGKDIKVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMK 194
SK ++ A K+IKVIV ++RP ++G+ A+ L + G+ V+Y + ++
Sbjct: 405 SSKVLTELLLHNAISLKKNIKVIVVDSRPLFEGRKMAETLRNAGVNVMYALITSLDTIFN 464
Query: 195 M-TDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEI 253
M D V +GA SI +NG + ++ GT+++A++AK + V++ E+ KF L +
Sbjct: 465 MDVDYVFLGAHSILSNGFLYSRAGTAMLAMSAKRRNIPVLVCCESLKFSQRVQLDSVTFN 524
Query: 254 EMRDPTEVI 262
E+ DP +++
Sbjct: 525 ELADPNDLV 533
>gb|AAF02141.1|AC009853_1 (AC009853) putative translation initiation factor EIF-2B beta
subunit, 3' partial [Arabidopsis thaliana]
Length = 349
Score = 92.4 bits (226), Expect = 6e-18
Identities = 62/212 (29%), Positives = 103/212 (48%), Gaps = 24/212 (11%)
Query: 70 SLPNALRYVMHRAKIAY--SSGADLET-----------LRFTVINSAKEFIHNSEKAIER 116
SL L A + Y SSGAD E+ L+ VI + I E+
Sbjct: 138 SLQTLLEGTPESATVPYTSSSGADSESKTADKSSLTRKLKHDVIEGVNQLIQEIAGCHEQ 197
Query: 117 IGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAKELAS 176
I E + I +VI+T S+ + + A ++ + +V V E PR+QG + AKEL +
Sbjct: 198 IAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVAEGAPRYQGHLLAKELVA 257
Query: 177 YGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIAA 236
G+ + DSA + + V++GA ++ ANG VI +G ++ AL A++H V ++ A
Sbjct: 258 RGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGVIGPVGVNMAALAAQKHAVPFVVLA 317
Query: 237 ETYKF------HPETMLGQLVEIEMRDPTEVI 262
++K +PE +L E+R P+E++
Sbjct: 318 GSHKLCPLYPHNPEVLLN-----ELRSPSELL 344
>sp|Q90511|E2BB_FUGRU TRANSLATION INITIATION FACTOR EIF-2B BETA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) (S20I15) >gi|1117954|gb|AAC59777.1|
(U40756) S20i15 [Takifugu rubripes]
Length = 355
Score = 92.1 bits (225), Expect = 8e-18
Identities = 79/305 (25%), Positives = 129/305 (41%), Gaps = 30/305 (9%)
Query: 21 IRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMH 80
+RG+ + AR L ++ S +L +++ + L +P+ ++ N +R V+
Sbjct: 31 LRGSSETARETTALLRRITAQARWSSAGDLMEIIRKEGRRLIAAQPSETTVGNMIRRVLK 90
Query: 81 --RAKIAYSSGADLET--------------------------LRFTVINSAKEFIHNSEK 112
R + A S G+ E LR VI + E + E
Sbjct: 91 IIREEYARSRGSSEEADQQESLHKLLTSGGLSEENFRQHFAALRANVIEAINELLTELEG 150
Query: 113 AIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAK 172
+ I + I +VIMT S+ + +K A + K VIV E P QG A
Sbjct: 151 TTDNIAMQALEHIHSNEVIMTVGRSRTVEAFLKDAARKRK-FHVIVAECAPFCQGHKMAT 209
Query: 173 ELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWV 232
L++ GI + D+A M +KV++G ++ ANG + GT +AL AK H +
Sbjct: 210 SLSTAGIETTVIADAAIFAVMSRVNKVIIGTQTVLANGGLRAVNGTHTLALAAKHHSTPL 269
Query: 233 MIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVI 292
++ A +K P+ + + P EV+P E + K + V P FD PPE I +
Sbjct: 270 IVCAPMFKLSPQFPNEEDTFHKFVSPHEVLPFTEGEILSK-VNVHCPVFDYVPPELITLF 328
Query: 293 ITEKG 297
I+ G
Sbjct: 329 ISNIG 333
>gb|AAF61750.1|AF064872_1 (AF064872) translation initiation factor eIF-2B precursor
[Sciaenops ocellatus]
Length = 355
Score = 85.8 bits (209), Expect = 6e-16
Identities = 76/305 (24%), Positives = 127/305 (40%), Gaps = 30/305 (9%)
Query: 21 IRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVMH 80
+RG+ + AR L ++ S +L +++ + + +P+ ++ N +R V+
Sbjct: 31 LRGSAETARETTALLRRITAQARWNSAGDLMEIIRKEGRRMTAAQPSETTVGNMIRRVLK 90
Query: 81 --RAKIAYSSGADLET--------------------------LRFTVINSAKEFIHNSEK 112
R + A S G+ E L+ VI + E + E
Sbjct: 91 IIREEYARSRGSSEEADQQESLHKLLTSGGLSEENFRQHFAALKANVIEAINELLTELEG 150
Query: 113 AIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDIKVIVTETRPRWQGKITAK 172
+ I + I +VIMT S+ + +K A + K VIV E P QG A
Sbjct: 151 TTDNISMQALEHIHSNEVIMTIGRSRTVEAFLKDAARKRK-FHVIVAECAPFCQGHEMAT 209
Query: 173 ELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWV 232
L+ I + D+A M +KV++G ++ ANG + GT +AL AK H +
Sbjct: 210 NLSKDEIETTVIADAAIFAVMSRVNKVIIGTQTVLANGGLRAVNGTHTLALAAKHHSTPL 269
Query: 233 MIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVI 292
++ A +K P+ + + P EV+P E + K + V P FD PPE I +
Sbjct: 270 IVCAPMFKLSPQFPNEEDTFHKFVSPHEVLPFTEGEILSK-VNVHCPVFDYVPPELITLF 328
Query: 293 ITEKG 297
I+ G
Sbjct: 329 ISNIG 333
>gi|7657058 eukaryotic translation initiation factor 2B, subunit 2 (beta, 39kD)
[Homo sapiens] >gi|11434950|ref|XP_007427.1| eukaryotic
translation initiation factor 2B, subunit 2 (beta, 39kD)
[Homo sapiens] >gi|6226858|sp|P49770|E2BB_HUMAN
TRANSLATION INITIATION FACTOR EIF-2B BETA SUBUNIT
(EIF-2B GDP-GTP EXCHANGE FACTOR) (S20I15) (S20III15)
>gi|2661031|gb|AAB88176.1| (AF035280) similar to
translation initiation factor eIF-2B beta subunit [Homo
sapiens] >gi|4809338|gb|AAD30183.1|AC006530_5 (AC006530)
EIF-2B [Homo sapiens]
Length = 351
Score = 85.4 bits (208), Expect = 8e-16
Identities = 63/203 (31%), Positives = 94/203 (46%), Gaps = 2/203 (0%)
Query: 95 LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDI 154
L+ +I + E + E +E I + I +VIMT S+ + +K A + K
Sbjct: 129 LQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRK-F 187
Query: 155 KVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVIN 214
VIV E P QG A L+ GI + D+A M +KV++G +I ANGA+
Sbjct: 188 HVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRA 247
Query: 215 KIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNI 274
GT +AL AK H +++ A +K P+ + + P EV+P E K +
Sbjct: 248 VTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEK-V 306
Query: 275 EVWNPAFDVTPPEYIDVIITEKG 297
V P FD PPE I + I+ G
Sbjct: 307 SVHCPVFDYVPPELITLFISNIG 329
>emb|CAA19648.1| (AL023874) /match=(desc:""TRANSLATION INITIATION FACTOR EIF-2B BETA
SUBUNIT (EIF-2B GDP-GTP EXCHANGE FACTOR)"",
species:""Rattus norvegicus (Rat)"",
ranges:(query:11048..11176,
target:SWISS-PROT::Q62818:57..99, score:""96.00""),
(query:11240..11470, ta> >gi|7290350|gb|AAF45809.1|
(AE003425) EG:100G10.3 gene product [Drosophila
melanogaster] >gi|11559594|gb|AAG38015.1| (AF289101)
eukaryotic initiation factor eIF2B beta subunit
[Drosophila melanogaster]
Length = 352
Score = 85.4 bits (208), Expect = 8e-16
Identities = 58/217 (26%), Positives = 104/217 (47%), Gaps = 3/217 (1%)
Query: 95 LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDI 154
LR +++ +E E + E I + I ++I+T HS++ + +K A + + +
Sbjct: 129 LREALLDHLQEVETELETSSENICVQAEEHIHSSEIILTLGHSRSVENFLKRAIKKRQFL 188
Query: 155 KVIVTETRPRWQGKITAKELASY-GIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVI 213
+IV E P +G A LA+ + ++ + D+A M +KV++G S+ ANG +
Sbjct: 189 TIIVAECAPACRGHNLAASLANEKNVEIVVIPDAAIFAMMSRVNKVIIGTHSVLANGGLR 248
Query: 214 NKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKN 273
G +AL AK + V V++ A YK P + Q + +VIP + + +
Sbjct: 249 AACGAYTVALAAKHYSVPVIVLAPMYKLSPLHLCEQDAFNMVGCAEDVIPYDSIPA--RE 306
Query: 274 IEVWNPAFDVTPPEYIDVIITEKGIIPPYAAIDILKE 310
+V++P FD PPE + + I+ G P +L E
Sbjct: 307 AKVYSPMFDYVPPELVTLFISNTGGHAPSYVYRLLTE 343
>sp|Q62818|E2BB_RAT TRANSLATION INITIATION FACTOR EIF-2B BETA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7514108|pir||S71960 translation
initiation factor eIF-2B beta chain - rat
>gi|1143158|gb|AAB38753.1| (U31880) eIF-2B beta subunit
[Rattus norvegicus]
Length = 351
Score = 81.5 bits (198), Expect = 1e-14
Identities = 61/216 (28%), Positives = 96/216 (44%), Gaps = 2/216 (0%)
Query: 95 LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDI 154
L+ +I + E + E E I + I +VIMT S+ + ++ A + K
Sbjct: 129 LKSNIIEAINELLVELEGTTENIAAQALEHIHSNEVIMTIGFSRTVEAFLREAAQKRK-F 187
Query: 155 KVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVIN 214
VI E P QG A L+ GI + D+A M +KV++G +I ANG++
Sbjct: 188 HVIAAECAPFCQGHEMAVNLSEAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGSLRA 247
Query: 215 KIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNI 274
GT +AL AK H +++ A +K P+ + + P EV+P E K +
Sbjct: 248 VAGTHTLALAAKHHSTPLIVCAPMFKLSPQFPSEEDSFHKFVAPEEVLPFTEGDILEK-V 306
Query: 275 EVWNPAFDVTPPEYIDVIITEKGIIPPYAAIDILKE 310
V P FD PP+ I + I+ G P ++ E
Sbjct: 307 SVHCPVFDYVPPDLITLFISNIGGNAPSYVYRLMSE 342
>gb|AAB58527.1| (U83914) eukaryotic initiation factor eIF-2B beta subunit [Rattus
norvegicus]
Length = 348
Score = 81.5 bits (198), Expect = 1e-14
Identities = 61/216 (28%), Positives = 96/216 (44%), Gaps = 2/216 (0%)
Query: 95 LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDI 154
L+ +I + E + E E I + I +VIMT S+ + ++ A + K
Sbjct: 126 LKSNIIEAINELLVELEGTTENIAAQALEHIHSNEVIMTIGFSRTVEAFLREAAQKRK-F 184
Query: 155 KVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVIN 214
VI E P QG A L+ GI + D+A M +KV++G +I ANG++
Sbjct: 185 HVIAAECAPFCQGHEMAVNLSEAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGSLRA 244
Query: 215 KIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNI 274
GT +AL AK H +++ A +K P+ + + P EV+P E K +
Sbjct: 245 VAGTHTLALAAKHHSTPLIVCAPMFKLSPQFPSEEDSFHKFVAPEEVLPFTEGDILEK-V 303
Query: 275 EVWNPAFDVTPPEYIDVIITEKGIIPPYAAIDILKE 310
V P FD PP+ I + I+ G P ++ E
Sbjct: 304 SVHCPVFDYVPPDLITLFISNIGGNAPSYVYRLMSE 339
>sp|Q28690|E2BB_RABIT TRANSLATION INITIATION FACTOR EIF-2B BETA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|1363072|pir||S56172 translation
initiation factor eIF-2B beta chain - rabbit
>gi|1061200|emb|CAA88255.1| (Z48222) protein synthesis
initiation factor eIF-2B beta subunit [Oryctolagus
cuniculus]
Length = 351
Score = 79.6 bits (193), Expect = 5e-14
Identities = 60/203 (29%), Positives = 92/203 (44%), Gaps = 2/203 (0%)
Query: 95 LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISVMKTAFDQGKDI 154
L+ ++ + E + E E I + I +VIMT S+ + ++ A + K
Sbjct: 129 LQSNIVEAINELLVELEGTTENIAAQALEHIHSNEVIMTIGLSRTVEAFLREAARKRK-F 187
Query: 155 KVIVTETRPRWQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITANGAVIN 214
VIV E P QG A L+ GI + D+A M +KV++G +I ANGA+
Sbjct: 188 HVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRA 247
Query: 215 KIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNI 274
GT +AL AK H +++ A +K P+ + + P EV+P E K +
Sbjct: 248 VTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILDK-V 306
Query: 275 EVWNPAFDVTPPEYIDVIITEKG 297
P FD PPE I + I+ G
Sbjct: 307 GCHCPVFDYVPPELITLFISNIG 329
>pir||T38663 probable translation initiation factor eif-2b beta subunit -
fission yeast (Schizosaccharomyces pombe)
>gi|5706515|emb|CAB52277.1| (AL109739) putative
translation initiation factor eif-2b beta subunit
[Schizosaccharomyces pombe]
Length = 393
Score = 79.2 bits (192), Expect = 6e-14
Identities = 64/291 (21%), Positives = 123/291 (41%), Gaps = 16/291 (5%)
Query: 33 QALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNALRYVM---------HRAK 83
+ L++ + S++ S + ++ K++ P +V + +++ ++ H
Sbjct: 101 EKLIVSSSNSSSPSQKR---DIPSNEKLVQSHEPVSVQMYSSMLNLLGRPTLESPTHSKT 157
Query: 84 IAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHCHSKAAISV 143
+ S +R +I+ ++ I +K I + ++I+T SK +
Sbjct: 158 VGDSRVTGGMDMRAVIISGIQDVIDELDKINTDIEVQSMDHLHSNEIILTQGCSKTVEAF 217
Query: 144 MKTAFDQGKDIKVIVTETRPRWQ--GKITAKELASYGIPVIYVVDSAARHYMKMTDKVVM 201
++ A + K VIV E P Q AK LA GI + D+ M +KV++
Sbjct: 218 LRFAAKKRK-FSVIVAEGFPNNQKGSHAMAKRLAQAGIDTTVISDATIFAIMSRVNKVIL 276
Query: 202 GADSITANGAVINKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEV 261
G +I NG ++ G L+A A+ H V++ + YK P I++ P ++
Sbjct: 277 GTHAILGNGGLVTYSGAQLVAQAARHHATPVVVCSGIYKLSPVYPYDLESIIQLSSPDKI 336
Query: 262 IPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPPYAAIDILKEEF 312
+ E E+ NP +D PP+ +D+ IT G PP I+ + +
Sbjct: 337 MSFNE-GDLISRAEILNPYYDYIPPDLVDLFITNLGGYPPSYLYRIMNDTY 386
>gi|6323322 translation initiation factor eIF2b, 43 kDa subunit; negative
regulator of GCN4 expression; Gcd7p [Saccharomyces
cerevisiae] >gi|417036|sp|P32502|E2BB_YEAST TRANSLATION
INITIATION FACTOR EIF-2B DELTA SUBUNIT (EIF-2B GDP-GTP
EXCHANGE FACTOR) (GUANINE NUCLEOTIDE EXCHANGE FACTOR
SUBUNIT GCD7) (GCD COMPLEX SUBUNIT GCD7)
>gi|477773|pir||B48156 translation regulator GCD7 -
yeast (Saccharomyces cerevisiae)
>gi|171576|gb|AAA34634.1| (L07116) guanine nucleotide
exchange factor, eIF-2B, delta subunit [Saccharomyces
cerevisiae] >gi|596047|gb|AAB67337.1| (U17243) Subunit
of the guanine nucleotide exchange factor for EIF-2
[Saccharomyces cerevisiae]
Length = 381
Score = 76.9 bits (186), Expect = 3e-13
Identities = 79/334 (23%), Positives = 129/334 (37%), Gaps = 50/334 (14%)
Query: 15 KIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRPTAVSLPNA 74
K+K +++G+ IA Q LM + +L ++++ L K PTA S N
Sbjct: 28 KLKRRQVQGSYAIALETLQLLMRFISAARWNHVNDLIEQIRDLGNSLEKAHPTAFSCGNV 87
Query: 75 LRYVM---------------------------------------HRAKIAYSSGADLET- 94
+R ++ H+ + S + ++T
Sbjct: 88 IRRILAVLRDEVEEDTMSTTVTSTSVAEPLISSMFNLLQKPEQPHQNRKNSSGSSSMKTK 147
Query: 95 --LRFTVINSAKEFIHNSEKAIERIGEIGAKRIEDGDVIMTHC-HSKAAISVMKTAFDQG 151
R I K+ I + E I +I I D ++++T SK + + TA ++
Sbjct: 148 TDYRQVAIQGIKDLIDEIKNIDEGIQQIAIDLIHDHEILLTPTPDSKTVLKFLITARERS 207
Query: 152 -KDIKVIVTETRPR--WQGKITAKELASYGIPVIYVVDSAARHYMKMTDKVVMGADSITA 208
+ V+VTE P AK+LA + I + V DSA M KV++G ++
Sbjct: 208 NRTFTVLVTEGFPNNTKNAHEFAKKLAQHNIETLVVPDSAVFALMSRVGKVIIGTKAVFV 267
Query: 209 NGAVI-NKIGTSLIALTAKEHRVWVMIAAETYKFHPETMLGQLVEIEMRDPTEVIPEEEL 267
NG I + G S + A+E R V A YK P +E ++P +
Sbjct: 268 NGGTISSNSGVSSVCECAREFRTPVFAVAGLYKLSPLYPFDVEKFVEFGGSQRILPRMDP 327
Query: 268 KTWPKNIEVWNPAFDVTPPEYIDVIITEKGIIPP 301
+ K ++ N D PPE ID+ IT G P
Sbjct: 328 R---KRLDTVNQITDYVPPENIDIYITNVGGFNP 358
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.132 0.381
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 112903634
Number of Sequences: 2977
Number of extensions: 4494907
Number of successful extensions: 12520
Number of sequences better than 1.0e-10: 66
Number of HSP's better than 0.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 28
Number of HSP's that attempted gapping in prelim test: 12332
Number of HSP's gapped (non-prelim): 69
length of query: 324
length of database: 189,106,746
effective HSP length: 58
effective length of query: 266
effective length of database: 154,394,500
effective search space: 41068937000
effective search space used: 41068937000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 165 (68.7 bits)