BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2411 (top6A) DE:DNA topoisomerase VI, subunit A (top6A)
(382 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||F75179 DNA topoisomerase VI, chain A (top6a) PAB2411 - Pyro... 783 0.0
sp|O59209|TP6A_PYRHO TYPE II DNA TOPOISOMERASE VI SUBUNIT A 768 0.0
pir||C71034 probable typeII DNA topoisomerase subunit A - Pyroco... 608 e-173
sp|Q9YE67|TP6A_AERPE TYPE II DNA TOPOISOMERASE VI SUBUNIT A >gi|... 266 2e-70
sp|O05208|TP6A_SULSH TYPE II DNA TOPOISOMERASE VI SUBUNIT A >gi|... 253 2e-66
gb|AAK41243.1| DNA topoisomerase VI subunit A (top6A) [Sulfolobu... 252 6e-66
sp|O27089|TP6A_METTH TYPE II DNA TOPOISOMERASE VI SUBUNIT A >gi|... 234 2e-60
sp|Q57815|TP6A_METJA TYPE II DNA TOPOISOMERASE VI SUBUNIT A >gi|... 230 2e-59
gb|AAG19326.1| (AE005027) DNA topoisomerase VI subunit A; Top6A ... 226 3e-58
gi|11498545 DNA topoisomerase VI, subunit A (top6A) [Archaeoglob... 210 3e-53
pdb|1D3Y|B Chain B, Structure Of The Dna Topoisomerase Vi A Subu... 201 8e-51
pir||T48303 meiosis specific-like protein - Arabidopsis thaliana... 173 4e-42
emb|CAB81544.1| (AJ251989) putative topoisomerase VIA [Arabidops... 123 3e-27
emb|CAB99470.1| (AL135939) dJ481F12.2.1 (SPO11, meiotic protein ... 121 2e-26
gi|6912680 SPO11, meiotic protein covalently bound to DSB (S. ce... 120 2e-26
gi|6755624 sporulation protein, meiosis-specific, SPO11 homolog ... 120 4e-26
emb|CAB99469.1| (AL135939) dJ481F12.2.2 (SPO11, meiotic protein ... 114 2e-24
dbj|BAB01408.1| (AP000375) DNA topoisomerase VI, subunit A [Arab... 111 2e-23
sp|Q9WTK8|SP11_MOUSE SPO11 PROTEIN HOMOLOG >gi|5453472|gb|AAD435... 110 2e-23
gb|AAD43589.1|AF163054_1 (AF163054) meiotic DNA transesterase/to... 107 2e-22
gb|AAD49561.1|AF126400_1 (AF126400) SPO11 protein [Mus musculus]... 103 3e-21
emb|CAB81545.1| (AJ251990) putative topoisomerase VIA [Arabidops... 88 2e-16
gb|AAC61735.1| (AF085276) MEI-W68 [Drosophila melanogaster] >gi|... 75 1e-12
>pir||F75179 DNA topoisomerase VI, chain A (top6a) PAB2411 - Pyrococcus abyssi
(strain Orsay) >gi|5458027|emb|CAB49517.1| (AJ248284)
DNA topoisomerase VI, subunit A (top6A) [Pyrococcus
abyssi]
Length = 382
Score = 783 bits (2001), Expect = 0.0
Identities = 382/382 (100%), Positives = 382/382 (100%)
Query: 1 MKLKRQKPKEKFSYDPQKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARL 60
MKLKRQKPKEKFSYDPQKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARL
Sbjct: 1 MKLKRQKPKEKFSYDPQKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARL 60
Query: 61 IKLGDKLSRRYFLNVAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTREN 120
IKLGDKLSRRYFLNVAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTREN
Sbjct: 61 IKLGDKLSRRYFLNVAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTREN 120
Query: 121 TFEDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWA 180
TFEDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWA
Sbjct: 121 TFEDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWA 180
Query: 181 VPGTVEHIQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHR 240
VPGTVEHIQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHR
Sbjct: 181 VPGTVEHIQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHR 240
Query: 241 LHYEEGLPIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNL 300
LHYEEGLPIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNL
Sbjct: 241 LHYEEGLPIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNL 300
Query: 301 EHVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALAN 360
EHVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALAN
Sbjct: 301 EHVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALAN 360
Query: 361 KSLEFVAKEYLPEKIREGKLLP 382
KSLEFVAKEYLPEKIREGKLLP
Sbjct: 361 KSLEFVAKEYLPEKIREGKLLP 382
>sp|O59209|TP6A_PYRHO TYPE II DNA TOPOISOMERASE VI SUBUNIT A
Length = 382
Score = 768 bits (1961), Expect = 0.0
Identities = 371/382 (97%), Positives = 380/382 (99%)
Query: 1 MKLKRQKPKEKFSYDPQKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARL 60
MKLKRQKPKEKFSYDPQKVLKKLEDLAWKILEE KSGKNPYFDVPTRGLNNVYFDEE+RL
Sbjct: 1 MKLKRQKPKEKFSYDPQKVLKKLEDLAWKILEEAKSGKNPYFDVPTRGLNNVYFDEESRL 60
Query: 61 IKLGDKLSRRYFLNVAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTREN 120
I+LGD+LSRRYFLNVAHARKF QTLILMAYIKRLVSEGKHASLREAYYANKHT+PGTREN
Sbjct: 61 IRLGDRLSRRYFLNVAHARKFMQTLILMAYIKRLVSEGKHASLREAYYANKHTVPGTREN 120
Query: 121 TFEDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWA 180
TFEDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWA
Sbjct: 121 TFEDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWA 180
Query: 181 VPGTVEHIQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHR 240
VPGTVEHIQFPE+NVDYVLVVETAAMADRLIEEKYPK+ENCLIVATQGQASRGVRRLIHR
Sbjct: 181 VPGTVEHIQFPEINVDYVLVVETAAMADRLIEEKYPKRENCLIVATQGQASRGVRRLIHR 240
Query: 241 LHYEEGLPIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNL 300
LHYEEGLPIIVFTDGDPYGWYIYSTIK+GSINLAYLSEKLATPDAKFVGMTMDDIKEYNL
Sbjct: 241 LHYEEGLPIIVFTDGDPYGWYIYSTIKRGSINLAYLSEKLATPDAKFVGMTMDDIKEYNL 300
Query: 301 EHVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALAN 360
E+VTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQ+KEWQRQLKLALKWGVRIEQQALAN
Sbjct: 301 ENVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQDKEWQRQLKLALKWGVRIEQQALAN 360
Query: 361 KSLEFVAKEYLPEKIREGKLLP 382
KSLEFVAKEYLPEKIREGKLLP
Sbjct: 361 KSLEFVAKEYLPEKIREGKLLP 382
>pir||C71034 probable typeII DNA topoisomerase subunit A - Pyrococcus horikoshii
>gi|3257992|dbj|BAA30675.1| (AP000006) 301aa long
hypothetical typeII DNA topoisomerase subunit A
[Pyrococcus horikoshii]
Length = 301
Score = 608 bits (1552), Expect = e-173
Identities = 294/300 (98%), Positives = 300/300 (100%)
Query: 83 QTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERMLGVL 142
QTLILMAYIKRLVSEGKHASLREAYYANKHT+PGTRENTFEDQSESDPIIEDLERMLGVL
Sbjct: 2 QTLILMAYIKRLVSEGKHASLREAYYANKHTVPGTRENTFEDQSESDPIIEDLERMLGVL 61
Query: 143 REEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYVLVVE 202
REEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPE+NVDYVLVVE
Sbjct: 62 REEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEINVDYVLVVE 121
Query: 203 TAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPYGWYI 262
TAAMADRLIEEKYPK+ENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPYGWYI
Sbjct: 122 TAAMADRLIEEKYPKRENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPYGWYI 181
Query: 263 YSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKKGGPTGDY 322
YSTIK+GSINLAYLSEKLATPDAKFVGMTMDDIKEYNLE+VTEKLKGIPPDKKGGPTGDY
Sbjct: 182 YSTIKRGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLENVTEKLKGIPPDKKGGPTGDY 241
Query: 323 KRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIREGKLLP 382
KRLIEELNYPWFQ+KEWQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIREGKLLP
Sbjct: 242 KRLIEELNYPWFQDKEWQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIREGKLLP 301
>sp|Q9YE67|TP6A_AERPE TYPE II DNA TOPOISOMERASE VI SUBUNIT A >gi|7446569|pir||G72659
probable type II DNA topoisomerase VI subunit a APE0703
- Aeropyrum pernix (strain K1)
>gi|5104364|dbj|BAA79679.1| (AP000060) 385aa long
hypothetical type II DNA topoisomerase VI subunit a
[Aeropyrum pernix]
Length = 385
Score = 266 bits (674), Expect = 2e-70
Identities = 145/364 (39%), Positives = 218/364 (59%), Gaps = 20/364 (5%)
Query: 29 KILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVAHARKFTQTLILM 88
+++ +V G+ P ++P R L+N FD E ++ +G++ R FLNV +R+F QTL++
Sbjct: 24 RLISDVIKGRPPKLEIPKRTLSNTIFDPERGILVIGEEKLEREFLNVGESRRFMQTLLMA 83
Query: 89 AYIKRLVSEGKHASLREAYYANKHTIP-------GTRENTFEDQSESDPIIEDLERMLGV 141
+ I + + E ++ ++R+ YY KHTI ENT+++Q ESD +I+D+E G+
Sbjct: 84 SIIYQSLIENEYPTIRDLYYKGKHTIVYRDYSGRKREENTWDEQKESDSVIQDIEVYTGL 143
Query: 142 LREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYVLVV 201
RE+M I + +G + G++ I+ G D + SKLG G +A+ T + I+F +V+ ++VLVV
Sbjct: 144 FREDMLILSKEKGKVVGNMRIRSGGDVIDLSKLGHGAYAIEPTPDLIEFLDVDAEFVLVV 203
Query: 202 ETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPYGWY 261
E A+ +L + KK L+V GQ R RR + RLH E LP+ + TD DPYGWY
Sbjct: 204 EKDAVFQQLHRAGFWKKYKALLVTGSGQPDRATRRFVRRLHEELKLPVYIITDSDPYGWY 263
Query: 262 IYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDI------KEYNLEHVTEKLKGIPPDKK 315
IYS K GSI L+Y SE+LATP AKF+G+ M DI K Y E +K DK
Sbjct: 264 IYSVYKVGSITLSYESERLATPKAKFLGVQMTDIFGYRGKKPYLSEAERKKFMIKATDK- 322
Query: 316 GGPTGDYKRLIEELNYPWF-QNKEWQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEK 374
D KR E LNY W +N+ W +++L LK V++E +A+A+K L+F A +Y+PEK
Sbjct: 323 -----DIKRAKELLNYSWIGKNRRWNVEIRLFLKHLVKLEIEAIASKGLKFFAYQYIPEK 377
Query: 375 IREG 378
I G
Sbjct: 378 IETG 381
>sp|O05208|TP6A_SULSH TYPE II DNA TOPOISOMERASE VI SUBUNIT A >gi|1926404|emb|CAA71605.1|
(Y10582) type II DNA topoisomerase subunit A [Sulfolobus
shibatae]
Length = 389
Score = 253 bits (640), Expect = 2e-66
Identities = 139/374 (37%), Positives = 223/374 (59%), Gaps = 21/374 (5%)
Query: 17 QKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVA 76
+K L D ++E++K G+ ++P R L+N +DE+ +L+ LG+K RR FL++
Sbjct: 15 RKAANILRDKFLNLVEQLKKGEPLVMEIPMRTLSNAIYDEKRKLLLLGEKKLRRNFLDLN 74
Query: 77 HARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHT-----IPGTR----ENTFEDQSE 127
A++F QT+++ + I + ++ ++R+ YY KH+ I G + ENT+++Q E
Sbjct: 75 EAKRFMQTVLMASIIYDALVSDEYPTIRDLYYRGKHSLLLKSIEGNKIVSEENTWDEQKE 134
Query: 128 SDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEH 187
SD +I D+E +LREEM I + +G + G++ I+ G D + SK G G +A+ T +
Sbjct: 135 SDSVIVDIEVFTSLLREEMLILSKEKGKVVGNLRIRSGNDVIDLSKTGHGAYAIEPTPDL 194
Query: 188 IQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGL 247
I F +V+ ++VLVVE A+ +L + K+ +++ + GQ R RR + RL+ E L
Sbjct: 195 IDFIDVDAEFVLVVEKDAVFQQLHRAGFWKQYKSILITSAGQPDRATRRFVRRLNEELKL 254
Query: 248 PIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDI------KEYNLE 301
P+ + TD DPYGWYI+S + GSI+L+Y SE+LATPDAKF+G++M DI K Y E
Sbjct: 255 PVYILTDADPYGWYIFSVFRIGSISLSYESERLATPDAKFLGVSMGDIFGNSRKKPYLSE 314
Query: 302 HVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANK 361
E+ I K D KR E NY WF+ K WQ ++ L+ ++E +A+A+K
Sbjct: 315 --AERKNYIIKAK----DADIKRAEEIKNYEWFKTKAWQEEINTFLQRKAKLEIEAMASK 368
Query: 362 SLEFVAKEYLPEKI 375
L+F+A +Y+PEKI
Sbjct: 369 GLKFLAFQYIPEKI 382
>gb|AAK41243.1| DNA topoisomerase VI subunit A (top6A) [Sulfolobus solfataricus]
Length = 389
Score = 252 bits (636), Expect = 6e-66
Identities = 137/374 (36%), Positives = 223/374 (58%), Gaps = 21/374 (5%)
Query: 17 QKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVA 76
+K L D ++E++K G+ ++P R L+N +DE+ +L+ LG+K +R FL++
Sbjct: 15 RKAASILRDKFLNLVEQLKKGEPLVMEIPMRTLSNAIYDEKRKLLLLGEKKLKRNFLDMN 74
Query: 77 HARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHT-----IPGTR----ENTFEDQSE 127
A++F QT+++ + I + ++ ++R+ YY KH+ I G + ENT+++Q E
Sbjct: 75 EAKRFMQTVLMASIIYDALVSDEYPTIRDLYYRGKHSLLLKSIDGNKIVSEENTWDEQKE 134
Query: 128 SDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEH 187
SD +I D+E +LREEM I + +G + G++ I+ G D + SK G G +A+ T +
Sbjct: 135 SDSVIVDIEVFTSLLREEMLILSKEKGKVVGNLRIRSGNDTIDLSKTGHGAYAIEPTPDL 194
Query: 188 IQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGL 247
I F +V+ ++VLVVE A+ +L + K+ +++ + GQ R RR + RL+ E L
Sbjct: 195 IDFIDVDAEFVLVVEKDAVFQQLHRAGFWKQYKSILITSAGQPDRATRRFVRRLNEELKL 254
Query: 248 PIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDI------KEYNLE 301
P+ + TD DPYGWYI+S + GSI+L+Y SE+LATPDAKF+G++M DI K Y E
Sbjct: 255 PVYILTDADPYGWYIFSVFRIGSISLSYESERLATPDAKFLGVSMSDIFGNSRKKPYLSE 314
Query: 302 HVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANK 361
E+ I K+ D KR E NY WF+ K W+ ++ L ++E +A+A+K
Sbjct: 315 --AERKNYIIKAKE----ADIKRAEEIKNYEWFKTKAWEEEINTFLHRKAKLEIEAMASK 368
Query: 362 SLEFVAKEYLPEKI 375
L+F+A +Y+PEKI
Sbjct: 369 GLKFLAFQYIPEKI 382
>sp|O27089|TP6A_METTH TYPE II DNA TOPOISOMERASE VI SUBUNIT A >gi|7446566|pir||D69001
conserved hypothetical protein MTH1008 -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2622109|gb|AAB85504.1| (AE000873) conserved protein
[Methanobacterium thermoautotrophicum]
Length = 353
Score = 234 bits (590), Expect = 2e-60
Identities = 135/357 (37%), Positives = 207/357 (57%), Gaps = 18/357 (5%)
Query: 20 LKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVAHAR 79
+ KL+ L IL++V G+ P VP+RG +N+ +DE+ R LGD+ R NV +
Sbjct: 8 INKLKSLGDVILDDVTQGRIPRIKVPSRGTSNIIYDEDKRHYVLGDRYGTRSMGNVKQIK 67
Query: 80 KFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERML 139
K Q L + K LV+ K A+LRE YY ++ E F DQ ES+ + EDLE L
Sbjct: 68 KIGQMLYTANFCKDLVAREKTATLRELYYISEGW-----EVDFADQQESNIVGEDLEVTL 122
Query: 140 GVLREEMHITADRRGY-IYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYV 198
G+ REE+ + + G +YG + +++G+ E +A + G G+ + T++ ++F + +V+ V
Sbjct: 123 GMTREELGLMPEEDGASVYGALTVREGDIEIDALRSGKSGYNISPTIDEVEFVDHDVERV 182
Query: 199 LVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPY 258
+ VET M RL++EK KK + LIV +GQA+R RR I R++ E LP+ + DGDP+
Sbjct: 183 IAVETMGMFHRLVQEKAYKKFDALIVGLKGQAARATRRFIKRVNEELNLPVYICNDGDPW 242
Query: 259 GWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKKGGP 318
G++I I GS LA+++ +LATPDAKF+G+T DI Y+L T+ LK +
Sbjct: 243 GFHIAMVIISGSAKLAHVNHQLATPDAKFLGVTASDIINYDLP--TDPLKDV-------- 292
Query: 319 TGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKI 375
D RL E L P ++ W+ ++K L G + EQQ+ + LE+V YLPEK+
Sbjct: 293 --DVVRLKELLQDPRYRGDFWKTEIKKMLTIGKKAEQQSFSKYGLEYVVDTYLPEKL 347
>sp|Q57815|TP6A_METJA TYPE II DNA TOPOISOMERASE VI SUBUNIT A >gi|2128280|pir||A64346
hypothetical protein MJ0369 - Methanococcus jannaschii
>gi|1591076|gb|AAB98358.1| (U67490) DNA topoisomerase
VI, subunit A (top6A) [Methanococcus jannaschii]
Length = 369
Score = 230 bits (580), Expect = 2e-59
Identities = 143/372 (38%), Positives = 212/372 (56%), Gaps = 23/372 (6%)
Query: 13 SYDPQKVLK-KLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRY 71
S P+++ K K+ +LA K+ E++ GK P +P R L+N FD+E L K R
Sbjct: 8 SKKPREIAKQKIIELAKKMYEDLMKGKRPKITMPIRSLSNAMFDKEKGSFTLVGKEKART 67
Query: 72 FLNVAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPI 131
L V A+ F QT ++ + K+L+ ++LREAYY +K+ E F+DQ S+ +
Sbjct: 68 -LTVNQAKIFAQTTKMLEFAKQLLETDDFSTLREAYYVSKNW----GEARFDDQQASNNV 122
Query: 132 IEDLERMLGVLREEMHITADRRGY-IYGDIVI----KDGEDEFNASKLGTGGWAVPGTVE 186
IEDLE LGVLRE + + G + G + I +GE + +KLGTG + +P V
Sbjct: 123 IEDLEAALGVLREHLGFIPEEDGSSVVGPLKIIEETPEGELVVDCTKLGTGAYNIPNDVT 182
Query: 187 HIQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEG 246
+ E + D++L +ET+ M RL E++ K NC++V+ +G +R RR I RLH E
Sbjct: 183 KLNL-ETDADFILAIETSGMFARLNAERFWDKHNCILVSLKGVPARATRRFIKRLHEEHD 241
Query: 247 LPIIVFTDGDPYGWY-IYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTE 305
LP++VFTDGDPYG+ IY T+K GS +L++KL+ P A+ +G+T DI +Y+L
Sbjct: 242 LPVLVFTDGDPYGYLNIYRTLKVGSGKAIHLADKLSIPAARLIGVTPQDIIDYDLPTHPL 301
Query: 306 KLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSLEF 365
K + I K G D+ R ++P EWQ+ LK L GVR EQQ+LA L++
Sbjct: 302 KEQDIKRIKDGLKNDDFVR-----SFP-----EWQKALKQMLDMGVRAEQQSLAKYGLKY 351
Query: 366 VAKEYLPEKIRE 377
V YLPEKI++
Sbjct: 352 VVNTYLPEKIKD 363
>gb|AAG19326.1| (AE005027) DNA topoisomerase VI subunit A; Top6A [Halobacterium sp.
NRC-1]
Length = 366
Score = 226 bits (570), Expect = 3e-58
Identities = 134/374 (35%), Positives = 207/374 (54%), Gaps = 20/374 (5%)
Query: 10 EKFSYDPQKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSR 69
E + D + +L +A + ++ G P +PTR +N+ +DE+A + GD S
Sbjct: 3 ETHTSDETEARDQLLAIAEQFYDQFADGDIPRMSLPTRSKSNIEYDEDADVWVYGDSQST 62
Query: 70 RYFLNVAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESD 129
R +V ARK +++ + ++ + + EG+ ++LRE YY ++ E F DQSESD
Sbjct: 63 RSANSVRGARKLLKSVYTVDFLAQQLDEGRSSTLRELYYLSESW--DEAEAQFNDQSESD 120
Query: 130 PIIEDLERMLGVLREEMHITADRRGY-IYGDIVIKD----GEDEFNASK-LGTGGWAVPG 183
++EDLE + GV RE+ H+ + G + G + +++ G+ E + + +G GG+ +P
Sbjct: 121 KLVEDLEIVSGVKREDFHMRPEESGAKVMGPLRLREQTRRGDREIHCQEDVGQGGYQIPN 180
Query: 184 TVEHIQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHY 243
+ I F + + D+VL VET M DRL+E + N ++V GQ +R RRL RLH
Sbjct: 181 NPDTIDFLDTDADFVLCVETGGMRDRLVENGFDDDYNAIVVHLGGQPARATRRLTKRLHD 240
Query: 244 EEGLPIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHV 303
E LP+ VFTDGDP+ + IY ++ GSI A+LSE LATP A+F+G+ DI +Y+L
Sbjct: 241 ELDLPVTVFTDGDPWSYRIYGSVAYGSIKSAHLSEYLATPQAQFIGIRPQDIVDYDLP-- 298
Query: 304 TEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSL 363
T+ L D L EL P FQ+ W Q+ L L + EQQALA++ L
Sbjct: 299 TDPLS----------DSDVNALESELEDPRFQSDFWTEQIGLQLDIDKKAEQQALASRGL 348
Query: 364 EFVAKEYLPEKIRE 377
+FV YLPE++ E
Sbjct: 349 DFVTDTYLPERLAE 362
>gi|11498545 DNA topoisomerase VI, subunit A (top6A) [Archaeoglobus fulgidus]
>gi|3122956|sp|O29322|TP6A_ARCFU TYPE II DNA
TOPOISOMERASE VI SUBUNIT A >gi|7446567|pir||D69367 DNA
topoisomerase VI, subunit A (top6A) homolog -
Archaeoglobus fulgidus >gi|2649657|gb|AAB90299.1|
(AE001039) DNA topoisomerase VI, subunit A (top6A)
[Archaeoglobus fulgidus]
Length = 360
Score = 210 bits (528), Expect = 3e-53
Identities = 125/367 (34%), Positives = 207/367 (56%), Gaps = 22/367 (5%)
Query: 17 QKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVA 76
++ L+ L + I +++K+G+ P + TR N+ F+EE+ + GD+ S R V
Sbjct: 6 RRCLRALIGIVQNIYDQMKAGQVPELHIATRTKYNIEFNEESEVWVYGDRKSVRSAKTVK 65
Query: 77 HARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLE 136
A + + ++ ++K ++ K ++LRE YY ++ G FE+Q ESD ++EDLE
Sbjct: 66 GAYRILKMTYVIGFLKEQLNLNKSSTLRELYYISE----GWGAAKFEEQPESDRLVEDLE 121
Query: 137 RMLGVLREEMHITADRRGY-IYGDIVIKD----GEDEFNASK-LGTGGWAVPGTVEHIQF 190
+ RE HI + G + G + +++ G E + +G GG+ +P V+ I+F
Sbjct: 122 ILTNFQREHFHIRPEEDGATVIGPLRVREETRRGVREIHCQDDVGEGGYQIPVNVDKIEF 181
Query: 191 PEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPII 250
+ + +V+ +ET M DRL+E + +K + +IV +GQ +R RRL+ RL+ E LP++
Sbjct: 182 VDHDAKFVIAIETGGMRDRLVENGFDEKYDAIIVHLKGQPARSTRRLLRRLNTELNLPVV 241
Query: 251 VFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGI 310
VFTDGDP+ + I++++ GSI A+LSE LATP A+FVG+ DI +Y+L
Sbjct: 242 VFTDGDPWSYRIFASVAYGSIKSAHLSEYLATPAAQFVGIRPTDIVKYDL---------- 291
Query: 311 PPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSLEFVAKEY 370
P DK D K L L P F ++ W++++ L L+ + EQQALA L++V Y
Sbjct: 292 PADKL--TEEDIKALNAILTDPRFDSEFWKKEVNLQLEINKKSEQQALAKYGLDYVTDVY 349
Query: 371 LPEKIRE 377
LPE++ E
Sbjct: 350 LPERLSE 356
>pdb|1D3Y|B Chain B, Structure Of The Dna Topoisomerase Vi A Subunit
>gi|6573667|pdb|1D3Y|A Chain A, Structure Of The Dna
Topoisomerase Vi A Subunit
Length = 301
Score = 201 bits (507), Expect = 8e-51
Identities = 121/309 (39%), Positives = 179/309 (57%), Gaps = 21/309 (6%)
Query: 75 VAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIED 134
V A+ F QT ++ + K+L+ ++LREAYY +K+ E F+DQ S+ +IED
Sbjct: 2 VNQAKIFAQTTKMLEFAKQLLETDDFSTLREAYYVSKNW----GEARFDDQQASNNVIED 57
Query: 135 LERMLGVLREEMHITADRRGY-IYGDIVI----KDGEDEFNASKLGTGGWAVPGTVEHIQ 189
LE LGVLRE + + G + G + I +GE + +KLGTG + +P V +
Sbjct: 58 LEAALGVLREHLGFIPEEDGSSVVGPLKIIEETPEGELVVDCTKLGTGAYNIPNDVTKLN 117
Query: 190 FPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPI 249
E + D++L +ET+ M RL E++ K NC++V+ +G +R RR I RLH E LP+
Sbjct: 118 L-ETDADFILAIETSGMFARLNAERFWDKHNCILVSLKGVPARATRRFIKRLHEEHDLPV 176
Query: 250 IVFTDGDPYGWY-IYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLK 308
+VFTDGDPYG+ IY T+K GS +L++KL+ P A+ +G+T DI +Y+L K +
Sbjct: 177 LVFTDGDPYGYLNIYRTLKVGSGKAIHLADKLSIPAARLIGVTPQDIIDYDLPTHPLKEQ 236
Query: 309 GIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSLEFVAK 368
I K G D+ R ++P EWQ+ LK L GVR EQQ+LA L++V
Sbjct: 237 DIKRIKDGLKNDDFVR-----SFP-----EWQKALKQMLDMGVRAEQQSLAKYGLKYVVN 286
Query: 369 EYLPEKIRE 377
YLPEKI++
Sbjct: 287 TYLPEKIKD 295
>pir||T48303 meiosis specific-like protein - Arabidopsis thaliana
>gi|7413557|emb|CAB86036.1| (AL162973) meiosis
specific-like protein [Arabidopsis thaliana]
Length = 427
Score = 173 bits (433), Expect = 4e-42
Identities = 104/359 (28%), Positives = 186/359 (50%), Gaps = 16/359 (4%)
Query: 19 VLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVAHA 78
V ++E + +I + +G F VP+R +N + E I L DK + R F +V+
Sbjct: 81 VQTEIETVIVQIARSILAGDGFSFSVPSRAASNQLYVPELDRIVLKDKSTLRPFASVSSV 140
Query: 79 RKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERM 138
RK T T ++A I +L H + R+ +Y T F+DQ++SD +++D+ M
Sbjct: 141 RKTTITTRILALIHQLCLRNIHVTKRDLFY--------TDVKLFQDQTQSDAVLDDVSCM 192
Query: 139 LGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYV 198
LG R +++ A +G + G ++ D D + +K+G GG A+P ++ + + + ++
Sbjct: 193 LGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDAMFI 252
Query: 199 LVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPY 258
L+VE A RL E+++ + C+IV +GQ R + ++ E LP++ D DPY
Sbjct: 253 LLVEKDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPY 312
Query: 259 GWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKKGGP 318
G I S GS N++Y S L TPD K++G+ D+ +Y + +P ++
Sbjct: 313 GLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCR----LPMTEQDIK 368
Query: 319 TGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIRE 377
TG K ++EE +N W +L L +K + E QAL++ +++++ YLP K+++
Sbjct: 369 TG--KDMLEEDFVK--KNPGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQQ 423
>emb|CAB81544.1| (AJ251989) putative topoisomerase VIA [Arabidopsis thaliana]
Length = 362
Score = 123 bits (306), Expect = 3e-27
Identities = 88/364 (24%), Positives = 168/364 (45%), Gaps = 26/364 (7%)
Query: 19 VLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVA-- 76
+L++++D ++ ++ G++P + N + EA + DK + +
Sbjct: 13 LLQRIKDFTQSVVVDLAEGRSPKISI--NQFRNYCMNPEADCLCSSDKPKGQEIFTLKKE 70
Query: 77 -HARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDL 135
+ L ++ +++L+ E +HAS R+ YY + + F+ QS D I D+
Sbjct: 71 PQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHP--------SAFKAQSIVDRAIGDI 122
Query: 136 ERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNV 195
+ R +++ + G + G + ++ +F+ + VP VE ++
Sbjct: 123 CILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVSLA 182
Query: 196 DYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDG 255
+Y+LVVE + RL + + K C+++ +G RR + L + LP+ D
Sbjct: 183 EYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLVDC 242
Query: 256 DPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKK 315
DPYG+ I +T + GS+ +AY E L PD K++G D + Y+ +P
Sbjct: 243 DPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYS----------VPKQCL 292
Query: 316 GGPTGDYKRLIEELNYPWFQNKE---WQRQLKLALKWGVRIEQQALANKSLEFVAKEYLP 372
T + K+ E + + +E W+ +L+ LK GV+ E +AL+ SL F+++ Y+P
Sbjct: 293 LPLTEEDKKRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIP 352
Query: 373 EKIR 376
KIR
Sbjct: 353 SKIR 356
>emb|CAB99470.1| (AL135939) dJ481F12.2.1 (SPO11, meiotic protein covalently bound to
DSB (S. cerevisiae)-like (a DNA
transesterase/topoisomerase homolog isoform 1)) [Homo
sapiens]
Length = 395
Score = 121 bits (300), Expect = 2e-26
Identities = 94/379 (24%), Positives = 182/379 (47%), Gaps = 23/379 (6%)
Query: 5 RQKPKEKFSYDPQKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGL-NNVYFDEEARLIKL 63
R+ P +VL +E++ I+ + + P F + R N+ F++ L +
Sbjct: 32 REPPTGGSRLASSEVLASIENIIQDIITSLARNEAPAFTIDNRSSWENIKFEDSVGLQMV 91
Query: 64 GDKLSRRYFLNVAH-ARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTF 122
+R+ + A+KF+ L +++ I +LV +A+ R+ YY T F
Sbjct: 92 SHCTTRKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYY--------TDSQLF 143
Query: 123 EDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDI-VIKDGEDEFNASKLGTGGWAV 181
+Q+ D II D+ ML V R +HI + +G I G++ I++ + N + G AV
Sbjct: 144 GNQTVVDNIINDISCMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCT-CGATAVAV 202
Query: 182 PGTVEHIQFPEVNVDYVLVVETAAMADRLIEEKYPKKEN-CLIVATQGQASRGVRRLIHR 240
P ++ I+ + +VL+VE A RL+++ + K + C+++ +G R L+ +
Sbjct: 203 PSNIQGIRNLVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKK 262
Query: 241 LHYEEGLPIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNL 300
L +P+ D DP+G I K GS+++++ + L P +++G+ D+K N+
Sbjct: 263 LWDTFHVPVFTLVDADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNV 322
Query: 301 EHVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKE-WQRQLKLALKWGVRIEQQALA 359
K IP K+ D +L L P+ + W++++++ ++ E QAL
Sbjct: 323 ----PKDSLIPLTKR-----DQMKLDSILRRPYVTCQPFWRKEMEIMADSKMKAEIQALT 373
Query: 360 NKSLEFVAKEYLPEKIREG 378
S +++++ YLP K++ G
Sbjct: 374 FLSSDYLSRVYLPNKLKFG 392
>gi|6912680 SPO11, meiotic protein covalently bound to DSB (S. cerevisiae)-like
[Homo sapiens] >gi|11422023|ref|XP_009661.1| SPO11,
meiotic protein covalently bound to DSB (S.
cerevisiae)-like [Homo sapiens]
>gi|7674367|sp|Q9Y5K1|SP11_HUMAN SPO11 PROTEIN HOMOLOG
>gi|5532654|gb|AAD44812.1|AF149310_1 (AF149310) Spo11
homolog [Homo sapiens]
>gi|5815131|gb|AAD52562.1|AF169385_1 (AF169385) SPO11
[Homo sapiens]
Length = 396
Score = 120 bits (299), Expect = 2e-26
Identities = 92/366 (25%), Positives = 179/366 (48%), Gaps = 23/366 (6%)
Query: 18 KVLKKLEDLAWKILEEVKSGKNPYFDVPTRGL-NNVYFDEEARLIKLGDKLSRRYFLNVA 76
+VL +E++ I+ + + P F + R N+ F++ L + +R+ +
Sbjct: 46 EVLASIENIIQDIITSLARNEAPAFTIDNRSSWENIKFEDSVGLQMVSHCTTRKIKSDSP 105
Query: 77 H-ARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDL 135
A+KF+ L +++ I +LV +A+ R+ YY T F +Q+ D II D+
Sbjct: 106 KSAQKFSLILKILSMIYKLVQSNTYATKRDIYY--------TDSQLFGNQTVVDNIINDI 157
Query: 136 ERMLGVLREEMHITADRRGYIYGDI-VIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVN 194
ML V R +HI + +G I G++ I++ + N + G AVP ++ I+ +
Sbjct: 158 SCMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCT-CGATAVAVPSNIQGIRNLVTD 216
Query: 195 VDYVLVVETAAMADRLIEEKYPKKEN-CLIVATQGQASRGVRRLIHRLHYEEGLPIIVFT 253
+VL+VE A RL+++ + K + C+++ +G R L+ +L +P+
Sbjct: 217 AKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFHVPVFTLV 276
Query: 254 DGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPD 313
D DP+G I K GS+++++ + L P +++G+ D+K N+ K IP
Sbjct: 277 DADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNV----PKDSLIPLT 332
Query: 314 KKGGPTGDYKRLIEELNYPWFQNKE-WQRQLKLALKWGVRIEQQALANKSLEFVAKEYLP 372
K+ D +L L P+ + W++++++ ++ E QAL S +++++ YLP
Sbjct: 333 KR-----DQMKLDSILRRPYVTCQPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLP 387
Query: 373 EKIREG 378
K++ G
Sbjct: 388 NKLKFG 393
>gi|6755624 sporulation protein, meiosis-specific, SPO11 homolog (S. cervisiae)
[Mus musculus] >gi|5815133|gb|AAD52563.1|AF169386_1
(AF169386) SPO11 [Mus musculus]
>gi|9367101|gb|AAF87096.1|AF173848_1 (AF173848) SPO11
protein [Mus musculus]
Length = 396
Score = 120 bits (297), Expect = 4e-26
Identities = 88/365 (24%), Positives = 173/365 (47%), Gaps = 21/365 (5%)
Query: 18 KVLKKLEDLAWKILEEVKSGKNPYFDVPTRGL-NNVYFDEEARLIKLGDKLSRRYFLNVA 76
+VL +E++ I++ + + P F + R N+ FD+ L + +R+ +
Sbjct: 46 EVLTAIENIIQDIIKSLARNEVPAFTIDNRSSWENIMFDDSVGLRMIPQCTTRKIRSDSP 105
Query: 77 HA-RKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDL 135
+ +KF L +++ I +L+ +A+ R+ YY T F +Q+ D I+D+
Sbjct: 106 KSVKKFALILKVLSMIYKLIQSDTYATKRDIYY--------TDSQLFGNQAAVDSAIDDI 157
Query: 136 ERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNV 195
ML V R +H+ + +G I G++ + + AVP ++ +Q +
Sbjct: 158 SCMLKVPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCTCSATATAVPTNIQGMQHLITDA 217
Query: 196 DYVLVVETAAMADRLIEEKYPKKEN-CLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTD 254
++L+VE A RL+++ + + + C++V +G R L+ +L +P+ D
Sbjct: 218 KFLLIVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTFHIPVFTLVD 277
Query: 255 GDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDK 314
DPYG I K GS+++++ + L P +++G+ DI+ N+ K IP K
Sbjct: 278 ADPYGIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNI----PKDSLIPLTK 333
Query: 315 KGGPTGDYKRLIEELNYPWFQNKE-WQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPE 373
D +L L P+ + W+++L++ ++ E QAL S +++++ YLP
Sbjct: 334 H-----DQMKLDSILKRPYITYQPLWKKELEMMADSKMKAEIQALTLLSSDYLSRVYLPN 388
Query: 374 KIREG 378
K+R G
Sbjct: 389 KLRFG 393
>emb|CAB99469.1| (AL135939) dJ481F12.2.2 (SPO11, meiotic protein covalently bound to
DSB (S.cerevisiae)-like (meiotic DNA
transesterase/topoisomerase homolog isoform 2 (high
similarity to mouse variant))) [Homo sapiens]
Length = 357
Score = 114 bits (283), Expect = 2e-24
Identities = 89/348 (25%), Positives = 169/348 (47%), Gaps = 26/348 (7%)
Query: 35 KSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVAH-ARKFTQTLILMAYIKR 93
+ G+ P PT G F++ L + +R+ + A+KF+ L +++ I +
Sbjct: 29 RGGREP----PTGGSRLASFEDSVGLQMVSHCTTRKIKSDSPKSAQKFSLILKILSMIYK 84
Query: 94 LVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERMLGVLREEMHITADRR 153
LV +A+ R+ YY T F +Q+ D II D+ ML V R +HI + +
Sbjct: 85 LVQSNTYATKRDIYY--------TDSQLFGNQTVVDNIINDISCMLKVSRRSLHILSTSK 136
Query: 154 GYIYGDI-VIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYVLVVETAAMADRLIE 212
G I G++ I++ + N + G AVP ++ I+ + +VL+VE A RL++
Sbjct: 137 GLIAGNLRYIEEDGTKVNCT-CGATAVAVPSNIQGIRNLVTDAKFVLIVEKDATFQRLLD 195
Query: 213 EKYPKKEN-CLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPYGWYIYSTIKQGSI 271
+ + K + C+++ +G R L+ +L +P+ D DP+G I K GS+
Sbjct: 196 DNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFHVPVFTLVDADPHGIEIMCIYKYGSM 255
Query: 272 NLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKKGGPTGDYKRLIEELNY 331
++++ + L P +++G+ D+K N+ K IP K+ D +L L
Sbjct: 256 SMSFEAHHLTVPAIRWLGLLPSDLKRLNV----PKDSLIPLTKR-----DQMKLDSILRR 306
Query: 332 PWFQNKE-WQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIREG 378
P+ + W++++++ ++ E QAL S +++++ YLP K++ G
Sbjct: 307 PYVTCQPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLPNKLKFG 354
>dbj|BAB01408.1| (AP000375) DNA topoisomerase VI, subunit A [Arabidopsis thaliana]
Length = 357
Score = 111 bits (274), Expect = 2e-23
Identities = 90/365 (24%), Positives = 167/365 (45%), Gaps = 33/365 (9%)
Query: 19 VLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVAHA 78
+L++++D ++ ++ G++P + N + EA + DK + +
Sbjct: 13 LLQRIKDFTQSVVVDLAEGRSPKISI--NQFRNYCMNPEADCLCSSDKPKGQEIFTL--- 67
Query: 79 RKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERM 138
+K QT + +++L+ E +HAS R+ YY + + F+ QS D I D+ +
Sbjct: 68 KKEPQTYRI---VQQLLQENRHASKRDIYYMHP--------SAFKAQSIVDRAIGDICIL 116
Query: 139 LGVLREEMH----ITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVN 194
R ++ I + G + ++ +F+ + VP VE ++
Sbjct: 117 FQCSRYNLNVIHLIILLCCRLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVSL 176
Query: 195 VDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTD 254
+Y+LVVE + RL + + K C+++ +G RR + L + LP+ D
Sbjct: 177 AEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLVD 236
Query: 255 GDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDK 314
DPYG+ I +T + GS+ +AY E L PD K++G D + Y+ +P
Sbjct: 237 CDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYS----------VPKQC 286
Query: 315 KGGPTGDYKRLIEELNYPWFQNKE---WQRQLKLALKWGVRIEQQALANKSLEFVAKEYL 371
T + K+ E + + +E W+ +L+ LK GV+ E +AL+ SL F+++ Y+
Sbjct: 287 LLPLTEEDKKRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYI 346
Query: 372 PEKIR 376
P KIR
Sbjct: 347 PSKIR 351
>sp|Q9WTK8|SP11_MOUSE SPO11 PROTEIN HOMOLOG >gi|5453472|gb|AAD43588.1|AF163053_1
(AF163053) meiotic DNA transesterase/topoisomerase
homolog variant A [Mus musculus]
>gi|5453482|gb|AAD43591.1| (AF163052) meiotic DNA
transesterase/topoisomerase homolog variant A [Mus
musculus] >gi|5532652|gb|AAD44811.1|AF149309_1
(AF149309) Spo11 homolog [Mus musculus]
>gi|9367033|gb|AAF87090.1|AF165313_1 (AF165313) SPO11
[Mus musculus]
Length = 358
Score = 110 bits (273), Expect = 2e-23
Identities = 76/302 (25%), Positives = 145/302 (47%), Gaps = 19/302 (6%)
Query: 79 RKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERM 138
+KF L +++ I +L+ +A+ R+ YY T F +Q+ D I+D+ M
Sbjct: 71 KKFALILKVLSMIYKLIQSDTYATKRDIYY--------TDSQLFGNQAAVDSAIDDISCM 122
Query: 139 LGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYV 198
L V R +H+ + +G I G++ + + AVP ++ +Q + ++
Sbjct: 123 LKVPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCTCSATATAVPTNIQGMQHLITDAKFL 182
Query: 199 LVVETAAMADRLIEEKYPKKEN-CLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDP 257
L+VE A RL+++ + + + C++V +G R L+ +L +P+ D DP
Sbjct: 183 LIVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTFHIPVFTLVDADP 242
Query: 258 YGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKKGG 317
YG I K GS+++++ + L P +++G+ DI+ N+ K IP K
Sbjct: 243 YGIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNI----PKDSLIPLTKH-- 296
Query: 318 PTGDYKRLIEELNYPWFQNKE-WQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIR 376
D +L L P+ + W+++L++ ++ E QAL S +++++ YLP K+R
Sbjct: 297 ---DQMKLDSILKRPYITYQPLWKKELEMMADSKMKAEIQALTLLSSDYLSRVYLPNKLR 353
Query: 377 EG 378
G
Sbjct: 354 FG 355
>gb|AAD43589.1|AF163054_1 (AF163054) meiotic DNA transesterase/topoisomerase homolog variant
B [Mus musculus] >gi|5453483|gb|AAD43592.1| (AF163052)
meiotic DNA transesterase/topoisomerase homolog variant
B [Mus musculus]
Length = 354
Score = 107 bits (265), Expect = 2e-22
Identities = 76/302 (25%), Positives = 145/302 (47%), Gaps = 23/302 (7%)
Query: 79 RKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERM 138
+KF L +++ I +L+ +A+ R+ YY T F +Q+ D I+D+ M
Sbjct: 71 KKFALILKVLSMIYKLIQSDTYATKRDIYY--------TDSQLFGNQAAVDSAIDDISCM 122
Query: 139 LGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYV 198
L V R +H+ + +G I G++ + + AVP ++ +Q + ++
Sbjct: 123 LKVPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCTCSATATAVPTNIQGMQ----HAKFL 178
Query: 199 LVVETAAMADRLIEEKYPKKEN-CLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDP 257
L+VE A RL+++ + + + C++V +G R L+ +L +P+ D DP
Sbjct: 179 LIVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTFHIPVFTLVDADP 238
Query: 258 YGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKKGG 317
YG I K GS+++++ + L P +++G+ DI+ N+ K IP K
Sbjct: 239 YGIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNI----PKDSLIPLTKH-- 292
Query: 318 PTGDYKRLIEELNYPWFQNKE-WQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIR 376
D +L L P+ + W+++L++ ++ E QAL S +++++ YLP K+R
Sbjct: 293 ---DQMKLDSILKRPYITYQPLWKKELEMMADSKMKAEIQALTLLSSDYLSRVYLPNKLR 349
Query: 377 EG 378
G
Sbjct: 350 FG 351
>gb|AAD49561.1|AF126400_1 (AF126400) SPO11 protein [Mus musculus]
>gi|9367040|gb|AAF87094.1|AF167439_1 (AF167439) SPO11b
[Mus musculus]
Length = 371
Score = 103 bits (255), Expect = 3e-21
Identities = 76/311 (24%), Positives = 147/311 (46%), Gaps = 24/311 (7%)
Query: 79 RKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPG---------TRENTFEDQSESD 129
+KF L +++ I +L+ +A+ R +N H++ T F +Q+ D
Sbjct: 71 KKFALILKVLSMIYKLIQSDTYATKR----SNAHSVLTLHLHRDIYYTDSQLFGNQAAVD 126
Query: 130 PIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQ 189
I+D+ ML V R +H+ + +G I G++ + + AVP ++ +Q
Sbjct: 127 SAIDDISCMLKVPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCTCSATATAVPTNIQGMQ 186
Query: 190 FPEVNVDYVLVVETAAMADRLIEEKYPKKEN-CLIVATQGQASRGVRRLIHRLHYEEGLP 248
+ ++L+VE A RL+++ + + + C++V +G R L+ +L +P
Sbjct: 187 HLITDAKFLLIVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTFHIP 246
Query: 249 IIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLK 308
+ D DPYG I K GS+++++ + L P +++G+ DI+ N+ K
Sbjct: 247 VFTLVDADPYGIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNI----PKDS 302
Query: 309 GIPPDKKGGPTGDYKRLIEELNYPWFQNKE-WQRQLKLALKWGVRIEQQALANKSLEFVA 367
IP K D +L L P+ + W+++L++ ++ E QAL S ++++
Sbjct: 303 LIPLTKH-----DQMKLDSILKRPYITYQPLWKKELEMMADSKMKAEIQALTLLSSDYLS 357
Query: 368 KEYLPEKIREG 378
+ YLP K+R G
Sbjct: 358 RVYLPNKLRFG 368
>emb|CAB81545.1| (AJ251990) putative topoisomerase VIA [Arabidopsis thaliana]
Length = 383
Score = 87.8 bits (214), Expect = 2e-16
Identities = 77/325 (23%), Positives = 148/325 (44%), Gaps = 22/325 (6%)
Query: 61 IKLGDKLSRRYFLNVAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTREN 120
I L ++ N A+ F + +M +++ + K + RE +Y P +
Sbjct: 77 IFLSTSFTKSSLTNAKTAKAFVRVWKVMEICFQILLQEKRVTQRELFYKLLCDSP----D 132
Query: 121 TFEDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKD-GEDEFNASKLGTGGW 179
F Q E + ++D+ +L R + I A RG + G + +++ G++ + S G+ G+
Sbjct: 133 YFSSQIEVNRSVQDVVALLRCSRYSLGIMASSRGLVAGRLFLQEPGKEAVDCSACGSSGF 192
Query: 180 AVPGTVEHIQFPEVNVD--YVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRL 237
A+ G + + + D Y+++VE A+ RL+E++ C+ + +G R
Sbjct: 193 AITGDLNLLDNTIMRTDARYIIIVEKHAIFHRLVEDRVFNHIPCVFITAKGYPDIATRFF 252
Query: 238 IHRLHYE-EGLPIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIK 296
+HR+ LPI+V D +P G I T K GSI + + + A + K++G+ DD+
Sbjct: 253 LHRMSTTFPDLPILVLVDWNPAGLAILCTFKFGSIGMGLEAYRYAC-NVKWIGLRGDDL- 310
Query: 297 EYNLEHVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQ 356
NL + P D + + ++++E + +L L ++ G R E +
Sbjct: 311 --NLIPEESLVPLKPKDSQIAKSLLSSKILQE---------NYIEELSLMVQTGKRAEIE 359
Query: 357 ALANKSLEFVAKEYLPEKIREGKLL 381
AL ++ K Y+ KI +GK +
Sbjct: 360 ALYCHGYNYLGK-YIATKIVQGKYI 383
>gb|AAC61735.1| (AF085276) MEI-W68 [Drosophila melanogaster]
>gi|7302468|gb|AAF57553.1| (AE003795) mei-W68 gene
product [Drosophila melanogaster]
Length = 331
Score = 75.3 bits (182), Expect = 1e-12
Identities = 78/363 (21%), Positives = 146/363 (39%), Gaps = 39/363 (10%)
Query: 21 KKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVAHARK 80
+ +E +A ++L + G N VP NV E R + ++ SR
Sbjct: 6 ENIERIALELLSNLVHG-NATLSVPRNSSGNVI--SEYRRVSYNNRGSRH---------S 53
Query: 81 FTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERMLG 140
F + +++ + RL G ++R YY N + QS D+ RML
Sbjct: 54 FCVLIYMLSRVHRLQVRGGSFTVRGLYYDNPLLV--------RSQSRIAEARLDVCRMLR 105
Query: 141 VLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYVLV 200
+ I A +G + GD+ + + S L G +P E I E ++VL+
Sbjct: 106 TSPLSLGILAASKGLVAGDLRLLMTNGDVLDSSLYGGPLTLPTDPEKIDRIETLAEFVLI 165
Query: 201 VETAAMADRLIEEKY--PKKENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPY 258
VE ++ + L+ + +++ +G RR++HRL E L + D DP+
Sbjct: 166 VEKESVFESLLSRNVFGTFERRFILITGKGYPDCCTRRIVHRLTEENQLAAYILVDADPF 225
Query: 259 GWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKKGGP 318
G I + GS ++++ S+ L TP +++G+ +I V
Sbjct: 226 GVEIMLVYRHGSKSMSFSSQGLTTPALRWIGLHPSEIPALGTGAVAL------------V 273
Query: 319 TGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIREG 378
GD K++ + L + + ++ L+ ++ +A ++F+ +Y+P KI
Sbjct: 274 AGDNKKINDLL-----ARHDLEPGVRQELRMLQDVQLKAEIESVIDFLTDDYIPNKINRN 328
Query: 379 KLL 381
L
Sbjct: 329 LFL 331
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.317 0.138 0.403
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 153446881
Number of Sequences: 2977
Number of extensions: 6911377
Number of successful extensions: 15508
Number of sequences better than 1.0e-10: 23
Number of HSP's better than 0.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 15434
Number of HSP's gapped (non-prelim): 23
length of query: 382
length of database: 189,106,746
effective HSP length: 56
effective length of query: 326
effective length of database: 155,591,474
effective search space: 50722820524
effective search space used: 50722820524
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.6 bits)
S2: 166 (69.1 bits)