BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2420 (PAB2420) DE:ARYLMALONATE DECARBOXYLASE
(236 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||F75088 arylmalonate decarboxylase PAB2420 - Pyrococcus abys... 466 e-131
pir||H71056 probable arylmalonate decarboxylase - Pyrococcus hor... 408 e-113
pir||D71090 probable arylmalonate decarboxylase - Pyrococcus hor... 293 1e-78
pir||H72484 probable arylmalonate decarboxylase APE2520 - Aeropy... 245 4e-64
gb|AAK43043.1| Maleate cis-trans isomerase, probable (maiA) [Su... 180 1e-44
pir||JC7225 maleate isomerase (EC 5.2.1.1) - Bacillus stearother... 116 2e-25
pir||JC5709 maleate isomerase (EC 5.2.1.1) - Alcaligenes faecali... 112 4e-24
pir||JC7339 maleate isomerase (EC 5.2.1.1) - Serratia marcescens... 108 5e-23
sp|Q05115|AMDA_ALCBR ARYLMALONATE DECARBOXYLASE (AMDASE) >gi|280... 94 1e-18
emb|CAA36292.1| (X52071) unidentified product (195 AA) [Streptom... 88 8e-17
>pir||F75088 arylmalonate decarboxylase PAB2420 - Pyrococcus abyssi (strain
Orsay) >gi|5458523|emb|CAB50011.1| (AJ248286)
ARYLMALONATE DECARBOXYLASE [Pyrococcus abyssi]
Length = 236
Score = 466 bits (1187), Expect = e-131
Identities = 236/236 (100%), Positives = 236/236 (100%)
Query: 1 MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60
MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK
Sbjct: 1 MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60
Query: 61 LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120
LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV
Sbjct: 61 LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120
Query: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180
QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD
Sbjct: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180
Query: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPLGKLLREY 236
GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPLGKLLREY
Sbjct: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPLGKLLREY 236
>pir||H71056 probable arylmalonate decarboxylase - Pyrococcus horikoshii
>gi|3257567|dbj|BAA30250.1| (AP000005) 236aa long
hypothetical arylmalonate decarboxylase [Pyrococcus
horikoshii]
Length = 236
Score = 408 bits (1037), Expect = e-113
Identities = 195/236 (82%), Positives = 220/236 (92%)
Query: 1 MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60
MFGWRGRIGLIVPSSNTTMEME HS LPEGVSLHTSRMPLKS+T+EEL M+SYAIEAA
Sbjct: 1 MFGWRGRIGLIVPSSNTTMEMELHSFLPEGVSLHTSRMPLKSITDEELFKMNSYAIEAAS 60
Query: 61 LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120
LLADAGVEVIAFGC+SGSFIGG+DFEKELEMKIE+EVN+ETFTTSTAV+EAL++L+ Q++
Sbjct: 61 LLADAGVEVIAFGCSSGSFIGGQDFEKELEMKIEEEVNIETFTTSTAVLEALDVLDAQSL 120
Query: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180
++TPY DEINQ+EKEFLE NGFEV+DI+GL ++DN+EIGKLEPY+ YRLAKA F GD D
Sbjct: 121 LIVTPYSDEINQKEKEFLEGNGFEVLDIKGLNLTDNLEIGKLEPYVVYRLAKAMFAGDAD 180
Query: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPLGKLLREY 236
IFISCTN RTFEIIEKLERDLGVPV+TSNQATLWMALRQIDV+D+LPLGKLLREY
Sbjct: 181 AIFISCTNLRTFEIIEKLERDLGVPVITSNQATLWMALRQIDVRDQLPLGKLLREY 236
>pir||D71090 probable arylmalonate decarboxylase - Pyrococcus horikoshii
>gi|3257399|dbj|BAA30082.1| (AP000004) 238aa long
hypothetical arylmalonate decarboxylase [Pyrococcus
horikoshii]
Length = 238
Score = 293 bits (742), Expect = 1e-78
Identities = 146/236 (61%), Positives = 184/236 (77%), Gaps = 1/236 (0%)
Query: 1 MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60
M+GWR RIGLIVPSSNTTME EF + PEGVS+HT+RM L+ VTEE LL M +A +AA
Sbjct: 1 MYGWRRRIGLIVPSSNTTMEPEFWKMAPEGVSIHTARMRLREVTEESLLEMERHAKDAAL 60
Query: 61 LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120
LADA V+VI +GCTSGS I GK ++KE+ +E+ ++T TTSTAV+ ALN L++Q V
Sbjct: 61 ELADAEVDVIVYGCTSGSLIKGKGYDKEIAKNLEEASGIKTITTSTAVLNALNTLDIQKV 120
Query: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180
V TPYID +N+REKEFLEANG EV+ I+GLGI N EIG+ EP++AY+LA +T + D
Sbjct: 121 VVATPYIDSVNEREKEFLEANGIEVLRIKGLGIVKNTEIGRQEPWVAYKLALEVYTPEAD 180
Query: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPL-GKLLRE 235
G+FISCTNFRT EII+KLE +LGVPVVTSNQAT+W AL+ + VK+K + G LL+E
Sbjct: 181 GLFISCTNFRTIEIIDKLEVELGVPVVTSNQATMWYALKTMKVKEKYDMYGTLLKE 236
>pir||H72484 probable arylmalonate decarboxylase APE2520 - Aeropyrum pernix
(strain K1) >gi|5106225|dbj|BAA81536.1| (AP000064) 272aa
long hypothetical arylmalonate decarboxylase [Aeropyrum
pernix]
Length = 272
Score = 245 bits (618), Expect = 4e-64
Identities = 115/225 (51%), Positives = 162/225 (71%)
Query: 1 MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60
M+GWR RIGLIVPSSNTTME E + PEGVS+HT R+PL+ V+ EELL M Y+ A+
Sbjct: 36 MYGWRARIGLIVPSSNTTMEPEMWMMAPEGVSIHTGRVPLEKVSVEELLKMEEYSAIEAE 95
Query: 61 LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120
LA AGV++I +GCT+GS IGG ++ ++ K+ V+ TT+TAV+EAL L +
Sbjct: 96 KLATAGVDLIVYGCTTGSLIGGPGYDLKIADKLSSASGVQVVTTATAVVEALKALGASRI 155
Query: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180
V TPYI+E+N+RE+EFL +GF++VD + LG+ N++IG++ P+ YRLA++ D
Sbjct: 156 AVATPYIEEVNKREEEFLRHHGFDIVDFKSLGLLGNLDIGRVPPWRVYRLARSLSIEGAD 215
Query: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKD 225
IFISCTN RT E+I LERD G+PVV+SN A++WMAL+ + +++
Sbjct: 216 AIFISCTNLRTVEVIGLLERDTGLPVVSSNTASMWMALKTLGIRE 260
>gb|AAK43043.1| Maleate cis-trans isomerase, probable (maiA) [Sulfolobus
solfataricus]
Length = 242
Score = 180 bits (453), Expect = 1e-44
Identities = 94/228 (41%), Positives = 145/228 (63%), Gaps = 7/228 (3%)
Query: 7 RIGLIVPSSNTTMEMEFHSVLPE----GVSLHTSRMPLKSVTEEELLNMSSYAIEAAKLL 62
++G+I+PSSNTT+E EF +V + ++ H SR+ L++VT E+L M AA L
Sbjct: 5 KVGVIIPSSNTTVEYEFSNVFNQIKEYRITPHFSRIKLRNVTIEDLAEMERETERAASEL 64
Query: 63 ADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTVQV 122
+ ++I++ CT+GS G +E+ +IEK V TS +VI AL L+++ + +
Sbjct: 65 STILPQIISYACTTGSLFKGPKHHEEIIQRIEKIAKVPATATSGSVINALRRLDIKRLVL 124
Query: 123 ITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLA---KASFTGDV 179
+TPYI+E+N++E EF NGFE+V G+GI +NI+IG++ P Y A + GD
Sbjct: 125 LTPYIEEVNKKEIEFFSKNGFEIVKSAGMGIRENIKIGQVTPEEVYNFAVNVLRNVKGDY 184
Query: 180 DGIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKL 227
DG+FISCTN RTFEI+ +LE++ PV++SN ATLW L +I++ K+
Sbjct: 185 DGVFISCTNLRTFEILSRLEKETKKPVISSNSATLWETLYKINLNIKV 232
>pir||JC7225 maleate isomerase (EC 5.2.1.1) - Bacillus stearothermophilus
>gi|4760466|dbj|BAA77296.1| (AB015134) maleate cis-trans
isomerase [Bacillus stearothermophilus]
Length = 251
Score = 116 bits (289), Expect = 2e-25
Identities = 71/242 (29%), Positives = 128/242 (52%), Gaps = 15/242 (6%)
Query: 7 RIGLIVPSSNTTMEMEFHSVLP--------EGVSLHTSRMPLKSVTEEELLNMSSYAIEA 58
RIG+IVPSSNTTME E ++L E + H+SRM + VT+EEL M +
Sbjct: 6 RIGMIVPSSNTTMETEIPAMLQSRMKEIPEETFTFHSSRMRMMHVTKEELKKMDEESDRC 65
Query: 59 AKLLADAGVEVIAFGCTSGSFIGGKDFEKELEMKI-----EKEVNVETFTTSTAVIEALN 113
A L+DA +V+A+ C G + ++ E ++ + +++ A+I+ +
Sbjct: 66 AIELSDARCDVLAYACLVAIMCQGPGYHEKSEARLASLTAQNGGAAPVISSAGALIDGIR 125
Query: 114 ILEMQTVQVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKA 173
L + + +I PY+ + + E++ A+G EV D L I DN+E+G+ +P + K
Sbjct: 126 TLGAKKIALIAPYMKPLTNQVIEYITASGIEVTDSISLEIPDNLEVGRQDPMRLIEIVKN 185
Query: 174 SFTGDVDGIFIS-CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP-LGK 231
+ D + +S C + I+K++ LG+PV+++ +T++ L+ +++K +P G
Sbjct: 186 LDVSNADAVVLSACVQMPSLPAIQKVQDQLGLPVLSAATSTVYKILKSLNLKTYVPNAGS 245
Query: 232 LL 233
LL
Sbjct: 246 LL 247
>pir||JC5709 maleate isomerase (EC 5.2.1.1) - Alcaligenes faecalis
>gi|2575787|dbj|BAA23002.1| (AB005051) maleate cis-trans
isomerase [Alcaligenes faecalis]
Length = 253
Score = 112 bits (277), Expect = 4e-24
Identities = 69/236 (29%), Positives = 120/236 (50%), Gaps = 14/236 (5%)
Query: 7 RIGLIVPSSNTTMEMEFHSVLP--------EGVSLHTSRMPLKSVTEEELLNMSSYAIEA 58
RIG IVPSSNTTME E ++L E + H+SRM + V EEL M +
Sbjct: 5 RIGQIVPSSNTTMETEIPAMLQARYAEFPEERFTFHSSRMRMMHVNPEELKAMDIASDRC 64
Query: 59 AKLLADAGVEVIAFGCTSGSFIGGKDFEKELEMKI-----EKEVNVETFTTSTAVIEALN 113
A L+DA + V+A+ C G + + + ++ E V + +++ A+++ L
Sbjct: 65 AVELSDARMSVMAYACLVAIMAQGDGYHRVSQARLQNTVKENGVEIPVLSSAGALVDTLK 124
Query: 114 ILEMQTVQVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKA 173
+ V +ITPY+ + +R +++EA G EV D L +SDN+E+G L P K
Sbjct: 125 EFGYKKVSIITPYMKPLTKRVADYIEAEGIEVQDSISLEVSDNLEVGLLNPENLLEHVKR 184
Query: 174 SFTGDVDGIFIS-CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP 228
VD + +S C + I++ + +G PV+++ T++ L+ + ++ ++P
Sbjct: 185 LNHDGVDAVILSACVQMPSLPAIQRAQDQIGKPVLSAAVWTVYQMLKNLGLETRVP 240
>pir||JC7339 maleate isomerase (EC 5.2.1.1) - Serratia marcescens
>gi|8570038|dbj|BAA96747.1| (AB035810) maleate cis-trans
isomerase [Serratia marcescens]
Length = 250
Score = 108 bits (268), Expect = 5e-23
Identities = 65/235 (27%), Positives = 120/235 (50%), Gaps = 13/235 (5%)
Query: 7 RIGLIVPSSNTTMEMEFHSVL-------PEGVSLHTSRMPLKSVTEEELLNMSSYAIEAA 59
RIG IVPSSNTTME E ++L PE + H+SRM +K V +EEL M + + A
Sbjct: 6 RIGQIVPSSNTTMETEIPAMLGARQLIRPERFTFHSSRMRMKHVNKEELAAMDAESDRCA 65
Query: 60 KLLADAGVEVIAFGCTSGSFIGGKDFEKELEMKI-----EKEVNVETFTTSTAVIEALNI 114
L+DA V+V+ + C G + +E + ++ + + +++ A++ L +
Sbjct: 66 LELSDARVDVLGYACLVAIMAMGLGYHRESQARLAQVTKDNQAAAPVISSAGALVNGLKV 125
Query: 115 LEMQTVQVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKAS 174
+ + + ++ PY+ + Q ++++ G EV R L I DN+++G+ +P +
Sbjct: 126 IGAKRIALVAPYMKPLTQLVVDYIQHEGIEVKVWRALEIPDNLDVGRHDPARLPGIVAEM 185
Query: 175 FTGDVDGIFIS-CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP 228
+VD I +S C + + +E G PV+T+ AT + L ++++ +P
Sbjct: 186 DLREVDAIVLSACVQMPSLPAVPTVEAQTGKPVITAAIATTYAMLTALELEPIVP 240
>sp|Q05115|AMDA_ALCBR ARYLMALONATE DECARBOXYLASE (AMDASE) >gi|280047|pir||JS0754
arylmalonate decarboxylase (EC 4.1.1.-) - Bordetella
bronchiseptica >gi|477178|pir||A48374 arylmalonate
decarboxylase - Alcaligenes bronchisepticus
>gi|216205|dbj|BAA02419.1| (D13116) 'Arylmalonate
Decarboxylase (AMDase)' [Alcaligenes bronchisepticus]
>gi|298239|gb|AAC60426.1| (S54007) arylmalonate
decarboxylase, AMDase [Alcaligenes bronchisepticus, KU
1201, Peptide, 240 aa]
Length = 240
Score = 94.4 bits (231), Expect = 1e-18
Identities = 66/214 (30%), Positives = 104/214 (47%), Gaps = 3/214 (1%)
Query: 8 IGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAKLLADAGV 67
IG+IVP + + + + P+ + S + L SVT E + ++ A+ L G
Sbjct: 9 IGMIVPPAAGLVPADGARLYPD-LPFIASGLGLGSVTPEGYDAVIESVVDHARRLQKQGA 67
Query: 68 EVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTVQVITPYI 127
V++ TS SF G F L + + + + T STAV+ L L ++ V + T YI
Sbjct: 68 AVVSLMGTSLSFYRGAAFNAALTVAMREATGLPCTTMSTAVLNGLRALGVRRVALATAYI 127
Query: 128 DEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTG--DVDGIFIS 185
D++N+R FL R LGI+ + +++ L +F D DGI +S
Sbjct: 128 DDVNERLAAFLAEESLVPTGCRSLGITGVEAMARVDTATLVDLCVRAFEAAPDSDGILLS 187
Query: 186 CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALR 219
C T + I ++ER LGVPVV+S+ A W A+R
Sbjct: 188 CGGLLTLDAIPEVERRLGVPVVSSSPAGFWDAVR 221
>emb|CAA36292.1| (X52071) unidentified product (195 AA) [Streptomyces lividans]
Length = 195
Score = 88.2 bits (215), Expect = 8e-17
Identities = 56/169 (33%), Positives = 86/169 (50%), Gaps = 2/169 (1%)
Query: 65 AGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTVQVIT 124
AG + + + CTSGSF+ G D +++ + V +TS A + A L ++ V +
Sbjct: 19 AGADAVVWACTSGSFVHGWD-GAHAQVRALAQAAVPASSTSFAFVHAAKELGVRRVSIGA 77
Query: 125 PYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVDGIFI 184
Y D++ + +FL A G EV D+ GI E+G LA+A+ DV+ + +
Sbjct: 78 TYPDDVARLFGDFLGAAGIEVADVVSSGIITAAEVGTWTEAEVLALARAADRPDVEAVLL 137
Query: 185 SCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP-LGKL 232
T T I LE++LG PV+T+NQ T+W LR D + P LG L
Sbjct: 138 PDTALHTAAHIPALEKELGKPVLTANQVTIWEGLRLADRRVNAPELGAL 186
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.318 0.137 0.380
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81054964
Number of Sequences: 2977
Number of extensions: 3152506
Number of successful extensions: 8597
Number of sequences better than 1.0e-10: 10
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 8579
Number of HSP's gapped (non-prelim): 10
length of query: 236
length of database: 189,106,746
effective HSP length: 57
effective length of query: 179
effective length of database: 154,992,987
effective search space: 27743744673
effective search space used: 27743744673
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 164 (68.3 bits)