BLASTP 2.0.10 [Aug-26-1999]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= PAB2420 (PAB2420) DE:ARYLMALONATE DECARBOXYLASE
         (236 letters)

Database: ./suso.pep; /banques/blast2/nr.pep
           598,487 sequences; 189,106,746 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||F75088 arylmalonate decarboxylase PAB2420 - Pyrococcus abys...   466  e-131
pir||H71056 probable arylmalonate decarboxylase - Pyrococcus hor...   408  e-113
pir||D71090 probable arylmalonate decarboxylase - Pyrococcus hor...   293  1e-78
pir||H72484 probable arylmalonate decarboxylase APE2520 - Aeropy...   245  4e-64
gb|AAK43043.1| Maleate cis-trans isomerase, probable  (maiA) [Su...   180  1e-44
pir||JC7225 maleate isomerase (EC 5.2.1.1) - Bacillus stearother...   116  2e-25
pir||JC5709 maleate isomerase (EC 5.2.1.1) - Alcaligenes faecali...   112  4e-24
pir||JC7339 maleate isomerase (EC 5.2.1.1) - Serratia marcescens...   108  5e-23
sp|Q05115|AMDA_ALCBR ARYLMALONATE DECARBOXYLASE (AMDASE) >gi|280...    94  1e-18
emb|CAA36292.1| (X52071) unidentified product (195 AA) [Streptom...    88  8e-17

>pir||F75088 arylmalonate decarboxylase PAB2420 - Pyrococcus abyssi  (strain
           Orsay) >gi|5458523|emb|CAB50011.1| (AJ248286)
           ARYLMALONATE DECARBOXYLASE [Pyrococcus abyssi]
           Length = 236
           
 Score =  466 bits (1187), Expect = e-131
 Identities = 236/236 (100%), Positives = 236/236 (100%)

Query: 1   MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60
           MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK
Sbjct: 1   MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60

Query: 61  LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120
           LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV
Sbjct: 61  LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120

Query: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180
           QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD
Sbjct: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180

Query: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPLGKLLREY 236
           GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPLGKLLREY
Sbjct: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPLGKLLREY 236


>pir||H71056 probable arylmalonate decarboxylase - Pyrococcus horikoshii
           >gi|3257567|dbj|BAA30250.1| (AP000005) 236aa long
           hypothetical arylmalonate decarboxylase [Pyrococcus
           horikoshii]
           Length = 236
           
 Score =  408 bits (1037), Expect = e-113
 Identities = 195/236 (82%), Positives = 220/236 (92%)

Query: 1   MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60
           MFGWRGRIGLIVPSSNTTMEME HS LPEGVSLHTSRMPLKS+T+EEL  M+SYAIEAA 
Sbjct: 1   MFGWRGRIGLIVPSSNTTMEMELHSFLPEGVSLHTSRMPLKSITDEELFKMNSYAIEAAS 60

Query: 61  LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120
           LLADAGVEVIAFGC+SGSFIGG+DFEKELEMKIE+EVN+ETFTTSTAV+EAL++L+ Q++
Sbjct: 61  LLADAGVEVIAFGCSSGSFIGGQDFEKELEMKIEEEVNIETFTTSTAVLEALDVLDAQSL 120

Query: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180
            ++TPY DEINQ+EKEFLE NGFEV+DI+GL ++DN+EIGKLEPY+ YRLAKA F GD D
Sbjct: 121 LIVTPYSDEINQKEKEFLEGNGFEVLDIKGLNLTDNLEIGKLEPYVVYRLAKAMFAGDAD 180

Query: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPLGKLLREY 236
            IFISCTN RTFEIIEKLERDLGVPV+TSNQATLWMALRQIDV+D+LPLGKLLREY
Sbjct: 181 AIFISCTNLRTFEIIEKLERDLGVPVITSNQATLWMALRQIDVRDQLPLGKLLREY 236


>pir||D71090 probable arylmalonate decarboxylase - Pyrococcus horikoshii
           >gi|3257399|dbj|BAA30082.1| (AP000004) 238aa long
           hypothetical arylmalonate decarboxylase [Pyrococcus
           horikoshii]
           Length = 238
           
 Score =  293 bits (742), Expect = 1e-78
 Identities = 146/236 (61%), Positives = 184/236 (77%), Gaps = 1/236 (0%)

Query: 1   MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60
           M+GWR RIGLIVPSSNTTME EF  + PEGVS+HT+RM L+ VTEE LL M  +A +AA 
Sbjct: 1   MYGWRRRIGLIVPSSNTTMEPEFWKMAPEGVSIHTARMRLREVTEESLLEMERHAKDAAL 60

Query: 61  LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120
            LADA V+VI +GCTSGS I GK ++KE+   +E+   ++T TTSTAV+ ALN L++Q V
Sbjct: 61  ELADAEVDVIVYGCTSGSLIKGKGYDKEIAKNLEEASGIKTITTSTAVLNALNTLDIQKV 120

Query: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180
            V TPYID +N+REKEFLEANG EV+ I+GLGI  N EIG+ EP++AY+LA   +T + D
Sbjct: 121 VVATPYIDSVNEREKEFLEANGIEVLRIKGLGIVKNTEIGRQEPWVAYKLALEVYTPEAD 180

Query: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLPL-GKLLRE 235
           G+FISCTNFRT EII+KLE +LGVPVVTSNQAT+W AL+ + VK+K  + G LL+E
Sbjct: 181 GLFISCTNFRTIEIIDKLEVELGVPVVTSNQATMWYALKTMKVKEKYDMYGTLLKE 236


>pir||H72484 probable arylmalonate decarboxylase APE2520 - Aeropyrum pernix
           (strain K1) >gi|5106225|dbj|BAA81536.1| (AP000064) 272aa
           long hypothetical arylmalonate decarboxylase [Aeropyrum
           pernix]
           Length = 272
           
 Score =  245 bits (618), Expect = 4e-64
 Identities = 115/225 (51%), Positives = 162/225 (71%)

Query: 1   MFGWRGRIGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAK 60
           M+GWR RIGLIVPSSNTTME E   + PEGVS+HT R+PL+ V+ EELL M  Y+   A+
Sbjct: 36  MYGWRARIGLIVPSSNTTMEPEMWMMAPEGVSIHTGRVPLEKVSVEELLKMEEYSAIEAE 95

Query: 61  LLADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTV 120
            LA AGV++I +GCT+GS IGG  ++ ++  K+     V+  TT+TAV+EAL  L    +
Sbjct: 96  KLATAGVDLIVYGCTTGSLIGGPGYDLKIADKLSSASGVQVVTTATAVVEALKALGASRI 155

Query: 121 QVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVD 180
            V TPYI+E+N+RE+EFL  +GF++VD + LG+  N++IG++ P+  YRLA++      D
Sbjct: 156 AVATPYIEEVNKREEEFLRHHGFDIVDFKSLGLLGNLDIGRVPPWRVYRLARSLSIEGAD 215

Query: 181 GIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKD 225
            IFISCTN RT E+I  LERD G+PVV+SN A++WMAL+ + +++
Sbjct: 216 AIFISCTNLRTVEVIGLLERDTGLPVVSSNTASMWMALKTLGIRE 260


>gb|AAK43043.1| Maleate cis-trans isomerase, probable  (maiA) [Sulfolobus
           solfataricus]
           Length = 242
           
 Score =  180 bits (453), Expect = 1e-44
 Identities = 94/228 (41%), Positives = 145/228 (63%), Gaps = 7/228 (3%)

Query: 7   RIGLIVPSSNTTMEMEFHSVLPE----GVSLHTSRMPLKSVTEEELLNMSSYAIEAAKLL 62
           ++G+I+PSSNTT+E EF +V  +     ++ H SR+ L++VT E+L  M      AA  L
Sbjct: 5   KVGVIIPSSNTTVEYEFSNVFNQIKEYRITPHFSRIKLRNVTIEDLAEMERETERAASEL 64

Query: 63  ADAGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTVQV 122
           +    ++I++ CT+GS   G    +E+  +IEK   V    TS +VI AL  L+++ + +
Sbjct: 65  STILPQIISYACTTGSLFKGPKHHEEIIQRIEKIAKVPATATSGSVINALRRLDIKRLVL 124

Query: 123 ITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLA---KASFTGDV 179
           +TPYI+E+N++E EF   NGFE+V   G+GI +NI+IG++ P   Y  A     +  GD 
Sbjct: 125 LTPYIEEVNKKEIEFFSKNGFEIVKSAGMGIRENIKIGQVTPEEVYNFAVNVLRNVKGDY 184

Query: 180 DGIFISCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKL 227
           DG+FISCTN RTFEI+ +LE++   PV++SN ATLW  L +I++  K+
Sbjct: 185 DGVFISCTNLRTFEILSRLEKETKKPVISSNSATLWETLYKINLNIKV 232


>pir||JC7225 maleate isomerase (EC 5.2.1.1) - Bacillus stearothermophilus
           >gi|4760466|dbj|BAA77296.1| (AB015134) maleate cis-trans
           isomerase [Bacillus stearothermophilus]
           Length = 251
           
 Score =  116 bits (289), Expect = 2e-25
 Identities = 71/242 (29%), Positives = 128/242 (52%), Gaps = 15/242 (6%)

Query: 7   RIGLIVPSSNTTMEMEFHSVLP--------EGVSLHTSRMPLKSVTEEELLNMSSYAIEA 58
           RIG+IVPSSNTTME E  ++L         E  + H+SRM +  VT+EEL  M   +   
Sbjct: 6   RIGMIVPSSNTTMETEIPAMLQSRMKEIPEETFTFHSSRMRMMHVTKEELKKMDEESDRC 65

Query: 59  AKLLADAGVEVIAFGCTSGSFIGGKDFEKELEMKI-----EKEVNVETFTTSTAVIEALN 113
           A  L+DA  +V+A+ C       G  + ++ E ++     +        +++ A+I+ + 
Sbjct: 66  AIELSDARCDVLAYACLVAIMCQGPGYHEKSEARLASLTAQNGGAAPVISSAGALIDGIR 125

Query: 114 ILEMQTVQVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKA 173
            L  + + +I PY+  +  +  E++ A+G EV D   L I DN+E+G+ +P     + K 
Sbjct: 126 TLGAKKIALIAPYMKPLTNQVIEYITASGIEVTDSISLEIPDNLEVGRQDPMRLIEIVKN 185

Query: 174 SFTGDVDGIFIS-CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP-LGK 231
               + D + +S C    +   I+K++  LG+PV+++  +T++  L+ +++K  +P  G 
Sbjct: 186 LDVSNADAVVLSACVQMPSLPAIQKVQDQLGLPVLSAATSTVYKILKSLNLKTYVPNAGS 245

Query: 232 LL 233
           LL
Sbjct: 246 LL 247


>pir||JC5709 maleate isomerase (EC 5.2.1.1) - Alcaligenes faecalis
           >gi|2575787|dbj|BAA23002.1| (AB005051) maleate cis-trans
           isomerase [Alcaligenes faecalis]
           Length = 253
           
 Score =  112 bits (277), Expect = 4e-24
 Identities = 69/236 (29%), Positives = 120/236 (50%), Gaps = 14/236 (5%)

Query: 7   RIGLIVPSSNTTMEMEFHSVLP--------EGVSLHTSRMPLKSVTEEELLNMSSYAIEA 58
           RIG IVPSSNTTME E  ++L         E  + H+SRM +  V  EEL  M   +   
Sbjct: 5   RIGQIVPSSNTTMETEIPAMLQARYAEFPEERFTFHSSRMRMMHVNPEELKAMDIASDRC 64

Query: 59  AKLLADAGVEVIAFGCTSGSFIGGKDFEKELEMKI-----EKEVNVETFTTSTAVIEALN 113
           A  L+DA + V+A+ C       G  + +  + ++     E  V +   +++ A+++ L 
Sbjct: 65  AVELSDARMSVMAYACLVAIMAQGDGYHRVSQARLQNTVKENGVEIPVLSSAGALVDTLK 124

Query: 114 ILEMQTVQVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKA 173
               + V +ITPY+  + +R  +++EA G EV D   L +SDN+E+G L P       K 
Sbjct: 125 EFGYKKVSIITPYMKPLTKRVADYIEAEGIEVQDSISLEVSDNLEVGLLNPENLLEHVKR 184

Query: 174 SFTGDVDGIFIS-CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP 228
                VD + +S C    +   I++ +  +G PV+++   T++  L+ + ++ ++P
Sbjct: 185 LNHDGVDAVILSACVQMPSLPAIQRAQDQIGKPVLSAAVWTVYQMLKNLGLETRVP 240


>pir||JC7339 maleate isomerase (EC 5.2.1.1) - Serratia marcescens
           >gi|8570038|dbj|BAA96747.1| (AB035810) maleate cis-trans
           isomerase [Serratia marcescens]
           Length = 250
           
 Score =  108 bits (268), Expect = 5e-23
 Identities = 65/235 (27%), Positives = 120/235 (50%), Gaps = 13/235 (5%)

Query: 7   RIGLIVPSSNTTMEMEFHSVL-------PEGVSLHTSRMPLKSVTEEELLNMSSYAIEAA 59
           RIG IVPSSNTTME E  ++L       PE  + H+SRM +K V +EEL  M + +   A
Sbjct: 6   RIGQIVPSSNTTMETEIPAMLGARQLIRPERFTFHSSRMRMKHVNKEELAAMDAESDRCA 65

Query: 60  KLLADAGVEVIAFGCTSGSFIGGKDFEKELEMKI-----EKEVNVETFTTSTAVIEALNI 114
             L+DA V+V+ + C       G  + +E + ++     + +      +++ A++  L +
Sbjct: 66  LELSDARVDVLGYACLVAIMAMGLGYHRESQARLAQVTKDNQAAAPVISSAGALVNGLKV 125

Query: 115 LEMQTVQVITPYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKAS 174
           +  + + ++ PY+  + Q   ++++  G EV   R L I DN+++G+ +P     +    
Sbjct: 126 IGAKRIALVAPYMKPLTQLVVDYIQHEGIEVKVWRALEIPDNLDVGRHDPARLPGIVAEM 185

Query: 175 FTGDVDGIFIS-CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP 228
              +VD I +S C    +   +  +E   G PV+T+  AT +  L  ++++  +P
Sbjct: 186 DLREVDAIVLSACVQMPSLPAVPTVEAQTGKPVITAAIATTYAMLTALELEPIVP 240


>sp|Q05115|AMDA_ALCBR ARYLMALONATE DECARBOXYLASE (AMDASE) >gi|280047|pir||JS0754
           arylmalonate decarboxylase (EC 4.1.1.-) - Bordetella
           bronchiseptica >gi|477178|pir||A48374 arylmalonate
           decarboxylase - Alcaligenes bronchisepticus
           >gi|216205|dbj|BAA02419.1| (D13116) 'Arylmalonate
           Decarboxylase (AMDase)' [Alcaligenes bronchisepticus]
           >gi|298239|gb|AAC60426.1| (S54007) arylmalonate
           decarboxylase, AMDase [Alcaligenes bronchisepticus, KU
           1201, Peptide, 240 aa]
           Length = 240
           
 Score = 94.4 bits (231), Expect = 1e-18
 Identities = 66/214 (30%), Positives = 104/214 (47%), Gaps = 3/214 (1%)

Query: 8   IGLIVPSSNTTMEMEFHSVLPEGVSLHTSRMPLKSVTEEELLNMSSYAIEAAKLLADAGV 67
           IG+IVP +   +  +   + P+ +    S + L SVT E    +    ++ A+ L   G 
Sbjct: 9   IGMIVPPAAGLVPADGARLYPD-LPFIASGLGLGSVTPEGYDAVIESVVDHARRLQKQGA 67

Query: 68  EVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTVQVITPYI 127
            V++   TS SF  G  F   L + + +   +   T STAV+  L  L ++ V + T YI
Sbjct: 68  AVVSLMGTSLSFYRGAAFNAALTVAMREATGLPCTTMSTAVLNGLRALGVRRVALATAYI 127

Query: 128 DEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTG--DVDGIFIS 185
           D++N+R   FL          R LGI+    + +++      L   +F    D DGI +S
Sbjct: 128 DDVNERLAAFLAEESLVPTGCRSLGITGVEAMARVDTATLVDLCVRAFEAAPDSDGILLS 187

Query: 186 CTNFRTFEIIEKLERDLGVPVVTSNQATLWMALR 219
           C    T + I ++ER LGVPVV+S+ A  W A+R
Sbjct: 188 CGGLLTLDAIPEVERRLGVPVVSSSPAGFWDAVR 221


>emb|CAA36292.1| (X52071) unidentified product (195 AA) [Streptomyces lividans]
           Length = 195
           
 Score = 88.2 bits (215), Expect = 8e-17
 Identities = 56/169 (33%), Positives = 86/169 (50%), Gaps = 2/169 (1%)

Query: 65  AGVEVIAFGCTSGSFIGGKDFEKELEMKIEKEVNVETFTTSTAVIEALNILEMQTVQVIT 124
           AG + + + CTSGSF+ G D     +++   +  V   +TS A + A   L ++ V +  
Sbjct: 19  AGADAVVWACTSGSFVHGWD-GAHAQVRALAQAAVPASSTSFAFVHAAKELGVRRVSIGA 77

Query: 125 PYIDEINQREKEFLEANGFEVVDIRGLGISDNIEIGKLEPYIAYRLAKASFTGDVDGIFI 184
            Y D++ +   +FL A G EV D+   GI    E+G         LA+A+   DV+ + +
Sbjct: 78  TYPDDVARLFGDFLGAAGIEVADVVSSGIITAAEVGTWTEAEVLALARAADRPDVEAVLL 137

Query: 185 SCTNFRTFEIIEKLERDLGVPVVTSNQATLWMALRQIDVKDKLP-LGKL 232
             T   T   I  LE++LG PV+T+NQ T+W  LR  D +   P LG L
Sbjct: 138 PDTALHTAAHIPALEKELGKPVLTANQVTIWEGLRLADRRVNAPELGAL 186


  Database: ./suso.pep
    Posted date:  Jul 6, 2001  5:57 PM
  Number of letters in database: 840,471
  Number of sequences in database:  2977
  
  Database: /banques/blast2/nr.pep
    Posted date:  Dec 14, 2000 12:46 PM
  Number of letters in database: 188,266,275
  Number of sequences in database:  595,510
  
Lambda     K      H
   0.318    0.137    0.380 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81054964
Number of Sequences: 2977
Number of extensions: 3152506
Number of successful extensions: 8597
Number of sequences better than 1.0e-10: 10
Number of HSP's better than  0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 8579
Number of HSP's gapped (non-prelim): 10
length of query: 236
length of database: 189,106,746
effective HSP length: 57
effective length of query: 179
effective length of database: 154,992,987
effective search space: 27743744673
effective search space used: 27743744673
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 164 (68.3 bits)