BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2424 (leuB-1) DE:3-isopropylmalate dehydrogenase (leuB)
(354 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D75045 3-isopropylmalate dehydrogenase (leub) PAB2424 - Pyr... 712 0.0
pir||T29052 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Stre... 331 7e-90
sp|P94631|LEU3_CORGL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 321 8e-87
sp|O33117|LEU3_MYCLE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 303 3e-81
sp|P95313|LEU3_MYCTU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 300 2e-80
sp|P94929|LEU3_MYCBO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 298 6e-80
sp|Q58991|IDH_METJA ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCIN... 283 2e-75
pir||C64499 isocitrate dehydrogenase (EC 1.1.1.-) - Methanococcu... 282 4e-75
sp|P76251|TTUC_ECOLI PROBABLE TARTRATE DEHYDROGENASE (TDH) >gi|7... 282 5e-75
gi|10956836 3-isopropylmalate dehydrogenase [Sphingomonas aromat... 268 9e-71
sp|Q51945|TTUC_PSEPU TARTRATE DEHYDROGENASE (TDH) >gi|1084041|pi... 264 1e-69
sp|P70792|TTC4_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)... 262 4e-69
sp|P70787|TTC2_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC (TDH) ... 262 5e-69
gi|11498236 3-isopropylmalate dehydrogenase (leuB) [Archaeoglobu... 262 5e-69
sp|Q58130|LEU3_METJA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 260 2e-68
pir||H64389 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) - Met... 260 2e-68
pir||A69113 isocitrate dehydrogenase - Methanobacterium thermoau... 256 3e-67
sp|O34296|TTC3_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)... 254 1e-66
sp|Q44471|TTC1_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC (TDH) ... 254 1e-66
sp|O34295|TTC5_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)... 253 2e-66
sp|O66607|LEU3_AQUAE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 250 1e-65
sp|O27441|LEU3_METTH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 249 3e-65
dbj|BAB04789.1| (AP001510) tartrate dehydrogenase [Bacillus halo... 248 9e-65
sp|Q9WZ26|LEU3_THEMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 236 3e-61
pdb|1IDM| 3-Isopropylmalate Dehydrogenase, Loop-Deleted Chimera 234 1e-60
pdb|1XAC| Chimera Isopropylmalate Dehydrogenase Between Bacill... 233 2e-60
pdb|1GC8|A Chain A, The Crystal Structure Of Thermus Thermophilu... 233 3e-60
pdb|1DR0|A Chain A, Structure Of Modified 3-Isopropylmalate Dehy... 231 9e-60
pdb|1DR8|A Chain A, Structure Of Modified 3-Isopropylmalate Dehy... 231 9e-60
pdb|1DPZ|A Chain A, Stucture Of Modified 3-Isopropylmalate Dehyd... 231 9e-60
pdb|1G2U|A Chain A, The Structure Of The Mutant, A172v, Of 3-Iso... 231 9e-60
pir||S41223 3-isopropylmalate dehydrogenase - Thermus aquaticus ... 231 9e-60
pdb|1OSJ|A Chain A, Structure Of 3-Isopropylmalate Dehydrogenase... 231 9e-60
sp|P00351|LEU3_THETH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 231 1e-59
pdb|1GC9|A Chain A, The Crystal Structure Of Thermus Thermophilu... 230 2e-59
sp|P31958|LEU3_CLOPA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 229 3e-59
sp|P24098|LEU3_THEAQ 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 229 4e-59
pir||A26522 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Baci... 229 4e-59
sp|P05645|LEU3_BACSU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 229 4e-59
pdb|1WAL|A Chain A, 3-Isopropylmalate Dehydrogenase (Ipmdh) Muta... 228 6e-59
pir||G82070 3-isopropylmalate dehydrogenase VC2491 [imported] - ... 228 8e-59
sp|P12010|LEU3_BACCO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 226 2e-58
gb|AAA22554.1| (M33099) 3-isopropylmalate dehydrogenase [Bacillu... 226 2e-58
pir||B81270 3-isopropylmalate dehydrogenase (EC 1.1.1.85) Cj1718... 226 2e-58
sp|P42958|YCSA_BACSU HYPOTHETICAL 33.1 KD PROTEIN IN MTLD-SIPU I... 226 4e-58
pir||A64729 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Esch... 222 6e-57
sp|P30125|LEU3_ECOLI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 222 6e-57
sp|P37412|LEU3_SALTY 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 221 8e-57
sp|P43860|LEU3_HAEIN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 220 1e-56
gb|AAG31390.1| (AF197452) 3-isopropylmaltate dehydrogenase [Buch... 219 4e-56
dbj|BAB06776.1| (AP001517) 3-isopropylmalate dehydrogenase [Baci... 218 7e-56
gb|AAG31402.1| (AF197456) 3-isopropylmaltate dehydrogenase [Buch... 218 9e-56
sp|P48572|LEU3_BUCRP 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 217 1e-55
pir||D83255 3-isopropylmalate dehydrogenase PA3118 [imported] - ... 217 2e-55
sp|Q51375|LEU3_PSEAE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 217 2e-55
sp|P24015|LEU3_LEPIN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 216 3e-55
sp|Q02143|LEU3_LACLA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 216 3e-55
gb|AAG31387.1| (AF197451) 3-isopropylmaltate dehydrogenase [Buch... 216 3e-55
gb|AAG31384.1| (AF197450) 3-isopropylmaltate dehydrogenase [Buch... 216 4e-55
pir||S20606 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Salm... 215 6e-55
gi|10954451 isopropylmalate dehydrogenase [Buchnera aphidicola] ... 214 1e-54
dbj|BAB08299.1| (AB007650) 3-isopropylmalate dehydrogenase [Arab... 213 2e-54
pir||B75160 3-isopropylmalate dehydrogenase (leub) PAB0289 - Pyr... 213 2e-54
sp|P54354|LEU3_BACFR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 213 3e-54
emb|CAA72702.1| (Y11966) 3-isopropylmalate dehydrogenase [Buchne... 212 5e-54
gb|AAD21684.1| (AC004793) Strong similarity to gb|X59970 3-isopr... 212 5e-54
sp|P05644|LEU3_BACCA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 212 7e-54
pir||E82564 3-isopropylmalate dehydrogenase XF2372 [imported] - ... 212 7e-54
gi|10957108 3-isopropylmalate dehydrogenase [Buchnera sp. APS] >... 210 2e-53
gb|AAG31405.1| (AF197457) 3-isopropylmaltate dehydrogenase [Buch... 210 2e-53
sp|P56933|LEU3_BUCAI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 210 3e-53
emb|CAA71268.1| (Y10216) hypothetical 3-isopropylmalate dehydrog... 209 3e-53
gb|AAG31399.1| (AF197455) 3-isopropylmaltate dehydrogenase [Buch... 209 3e-53
sp|P29102|LEU3_BRANA 3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR (... 208 6e-53
gb|AAG31393.1| (AF197453) 3-isopropylmaltate dehydrogenase [Buch... 208 6e-53
gb|AAG31928.1|AF200469_2 (AF200469) leuB [Buchnera aphidicola] 208 6e-53
pir||E75368 probable isocitrate dehydrogenase - Deinococcus radi... 206 3e-52
pir||E81836 probable 3-isopropylmalate dehydrogenase (EC 1.1.1.8... 206 3e-52
pir||E81130 3-isopropylmalate dehydrogenase NMB1031 [imported] -... 205 7e-52
gb|AAG31396.1| (AF197454) 3-isopropylmaltate dehydrogenase [Buch... 205 7e-52
gb|AAK41021.1| 3-isopropylmalate dehydrogenase (leuB) [Sulfolobu... 205 9e-52
sp|P73960|LEU3_SYNY3 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 205 9e-52
emb|CAB57580.1| (Y18930) 3-isopropylmalate dehydrogenase [Sulfol... 205 9e-52
gi|10954459 3-isopropylmalate dehydrogenase [Buchnera aphidicola... 204 2e-51
sp|P50455|LEU3_SULS7 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 202 6e-51
pir||A82539 isocitrate dehydrogenase XF2596 [imported] - Xylella... 202 6e-51
sp|P41019|LEU3_BACME 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 201 1e-50
gi|6322097 Homo-isocitrate dehydrogenase; Lys12p [Saccharomyces ... 201 1e-50
sp|P33197|IDH_THETH ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCIN... 199 3e-50
pir||A43934 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) - The... 199 3e-50
gb|AAG31378.1| (AF197448) 3-isopropylmaltate dehydrogenase [Buch... 198 9e-50
sp|P50180|LEU3_NEILA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 197 2e-49
gb|AAG31381.1| (AF197449) 3-isopropylmaltate dehydrogenase [Buch... 197 2e-49
sp|Q56268|LEU3_THIFE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 195 6e-49
sp|Q01987|LEU3_CANBO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 195 8e-49
pir||E71180 probable 3-isopropylmalate dehydrogenase - Pyrococcu... 192 5e-48
gb|AAB91428.1| (U86815) beta-isopropylmalate dehydrogenase [Expr... 192 7e-48
pir||T38621 probable isopropyl malate dehydrogenase - fission ye... 191 1e-47
pir||DEBYI 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - yeast... 190 1e-47
gb|AAB49959.1| (U70024) LEU2 [Cloning vector pGAD-C1] >gi|159584... 190 1e-47
gi|10383772 beta-IPM (isopropylmalate) dehydrogenase; Leu2p [Sac... 190 2e-47
sp|P41926|LEU3_YAMOH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 190 3e-47
emb|CAA60637.1| (X87172) NAD+-isocitrate dehydrogenase, alpha su... 190 3e-47
sp|Q28480|IDHA_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPH... 190 3e-47
sp|P04173|LEU3_YEAST 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 189 6e-47
gb|AAD56647.1|AF178973_2 (AF178973) dLEU2 [Cloning vector pCS512... 189 6e-47
sp|P07139|LEU3_CANMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 188 7e-47
sp|Q00412|LEU3_SPIPL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 188 7e-47
prf||1401173A C-LEU2 gene [Candida maltosa] 188 7e-47
pir||S48226 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (allel... 188 7e-47
pir||S48225 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (allel... 188 7e-47
pir||A44851 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Spir... 188 7e-47
sp|P41563|IDHA_BOVIN ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPH... 188 7e-47
sp|P23390|LEU3_KLULA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 187 2e-46
emb|CAB72262.1| (AJ271154) 3-isopropylmalate dehydrogenase [Phae... 187 2e-46
sp|P08791|LEU3_PICJA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 187 2e-46
emb|CAB99456.1| (AJ292544) beta-isopropylmalate dehydrogenase [Z... 186 3e-46
emb|CAC08508.1| (AJ294714) beta-isopropylmalate dehydrogenase [P... 186 4e-46
gb|AAA62618.1| (L14756) 3-isopropylmalate dehydrogenase [Cloning... 185 5e-46
gb|AAF48965.1| (AE003512) CG12233 gene product [Drosophila melan... 185 5e-46
pir||T48350 3-isopropylmalate dehydrogenase-like protein - Arabi... 185 6e-46
sp|P34733|LEU3_PICAN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 185 9e-46
sp|P18120|LEU3_YARLI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 184 1e-45
emb|CAC10274.1| (AJ279020) putative beta-isopropylmalate dehydro... 184 1e-45
gb|AAC53643.1| (U38900) 3-isopropylmalate dehydrogenase [Cloning... 184 1e-45
sp|P87186|LEU3_CANAL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 183 2e-45
pir||T03406 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.4... 183 2e-45
prf||2004294A beta isopropylmalate dehydrogenase [Neurospora cra... 182 4e-45
sp|P29696|LEU3_SOLTU 3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR (... 182 7e-45
sp|P34738|LEU3_NEUCR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 182 7e-45
dbj|BAA88179.1| (AP000836) ESTs C19133(E10008),AU058199(E50136),... 181 1e-44
pir||S30897 3-isopropylmalate dehydrogenase (EC 1.1.1.85) precur... 181 1e-44
pir||T50386 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.4... 180 2e-44
sp|O60027|LEU3_ASHGO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 180 2e-44
sp|P24404|LEU3_AGRTU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 180 3e-44
pir||G75355 3-isopropylmalate dehydrogenase - Deinococcus radiod... 180 3e-44
gb|AAF23254.1|AC015985_12 (AC015985) putative isocitrate dehydro... 179 4e-44
sp|P41766|LEU3_KLUMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 177 1e-43
sp|O14429|LEU3_CANGA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 176 2e-43
sp|Q12545|LEU3_CEPAC 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 176 4e-43
sp|P87256|LE3A_ASPNG 3-ISOPROPYLMALATE DEHYDROGENASE A (BETA-IPM... 176 4e-43
sp|Q9Y897|LEU3_MYCGR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 175 7e-43
sp|Q48806|DLPA_LEGPN DLPA PROTEIN >gi|2120838|pir||S61390 dlpA p... 175 1e-42
sp|P87257|LE3B_ASPNG 3-ISOPROPYLMALATE DEHYDROGENASE B (BETA-IPM... 175 1e-42
sp|O59930|LEU3_PHACH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 175 1e-42
sp|O94114|LEU3_PICST 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 173 2e-42
sp|P18869|LEU3_SCHPO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 171 8e-42
gb|AAC69609.1| (AF045154) NAD-dependent isocitrate dehydrogenase... 171 8e-42
sp|P96197|LEU3_AZOVI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM D... 170 2e-41
gi|6324709 NAD+-dependent isocitrate dehydrogenase; Idh2p [Sacch... 169 3e-41
emb|CAA74777.1| (Y14432) NAD-dependent isocitrate dehydrogenase ... 165 8e-40
gb|AAB81675.1| (AC002354) putative NAD+ dependent isocitrate deh... 164 1e-39
gb|AAC49965.1| (U81994) NAD+ dependent isocitrate dehydrogenase ... 164 2e-39
sp|Q93353|IDHB_CAEEL PROBABLE ISOCITRATE DEHYDROGENASE [NAD] SUB... 163 2e-39
gb|AAB63461.1| (AF009036) NAD(+)-isocitrate dehydrogenase subuni... 161 9e-39
sp|Q59985|IDH_STRSL ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCIN... 161 2e-38
pir||T06131 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) chain ... 160 2e-38
emb|CAA74776.1| (Y14431) NAD-dependent isocitrate dehydrogenase ... 159 5e-38
emb|CAC11264.1| (AL445063) probable isocitrate dehydrogenase [Th... 159 6e-38
pir||T04670 isocitrate dehydrogenase (NAD+) homolog F8D20.160 - ... 158 8e-38
sp|Q28479|IDHB_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA... 158 1e-37
gb|AAD09339.1| (AF023265) NAD+-specific isocitrate dehydrogenase... 157 2e-37
emb|CAC01443.1| (AL049712) dJ686C3.1.1 (isocitrate dehydrogenase... 157 2e-37
gi|5901982 isocitrate dehydrogenase 3 (NAD+) beta [Homo sapiens]... 156 4e-37
pir||T13147 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) beta c... 156 4e-37
emb|CAC01442.1| (AL049712) dJ686C3.1.2 (isocitrate dehydrogenase... 156 4e-37
gi|11421208 isocitrate dehydrogenase 3 (NAD+) beta [Homo sapiens] 156 4e-37
pir||T37546 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.4... 155 5e-37
gi|6324291 alpha-4-beta-4 subunit of mitochondrial isocitrate de... 155 9e-37
pir||A72658 probable isocitrate dehydrogenase APE0689 - Aeropyru... 153 3e-36
gb|AAC69608.1| (AF045153) NAD-dependent isocitrate dehydrogenase... 150 3e-35
gb|AAD55084.1| (AF176568) isocitrate dehydrogenase gamma subunit... 148 1e-34
dbj|BAA13177.2| (D86855) isocitrate dehydrogenase [Caldococcus n... 148 1e-34
pir||T44658 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) [vali... 148 1e-34
sp|P39126|IDH_BACSU ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCIN... 146 3e-34
pir||T08743 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.4... 140 3e-32
gi|4758582 isocitrate dehydrogenase 3 (NAD+) gamma [Homo sapiens... 134 1e-30
gi|11419927 isocitrate dehydrogenase 3 (NAD+) gamma [Homo sapiens] 134 1e-30
sp|P41564|IDHG_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT GAMM... 134 1e-30
gi|6680345 isocitrate dehydrogenase 3 (NAD+), gamma [Mus musculu... 133 3e-30
emb|CAA52225.1| (X74125) NAD+-isocitrate dehydrogenase, gamma su... 132 4e-30
pir||S39064 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) gamma ... 132 4e-30
sp|P41565|IDHG_RAT ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT GAMMA,... 132 4e-30
gb|AAB39334.1| (U55996) isocitrate dehydrogenase [Thermus aquati... 132 8e-30
dbj|BAA91971.1| (AK001905) unnamed protein product [Homo sapiens] 120 3e-26
pir||T39871 3-isopropylmalate dehydrogenase - fission yeast (Sch... 118 1e-25
gb|AAB70116.1| (U69269) NAD (H)-specific isocitrate dehydrogenas... 106 5e-22
pir||T07167 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.4... 104 1e-21
gb|AAF20164.1| (AF046929) NAD+-specific isocitrate dehydrogenase... 86 5e-16
>pir||D75045 3-isopropylmalate dehydrogenase (leub) PAB2424 - Pyrococcus abyssi
(strain Orsay) >gi|5458770|emb|CAB50257.1| (AJ248287)
3-isopropylmalate dehydrogenase (leuB) [Pyrococcus
abyssi]
Length = 354
Score = 712 bits (1819), Expect = 0.0
Identities = 354/354 (100%), Positives = 354/354 (100%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI
Sbjct: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK
Sbjct: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV
Sbjct: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVVTPNMFG 240
TLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVVTPNMFG
Sbjct: 181 TLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVVTPNMFG 240
Query: 241 DILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLE 300
DILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLE
Sbjct: 241 DILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLE 300
Query: 301 YLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLEVVANEEEG 354
YLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLEVVANEEEG
Sbjct: 301 YLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLEVVANEEEG 354
>pir||T29052 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Streptomyces
coelicolor >gi|3355670|emb|CAA19970.1| (AL031124)
3-isopropylmalate dehydrogenase [Streptomyces coelicolor
A3(2)]
Length = 347
Score = 331 bits (840), Expect = 7e-90
Identities = 179/340 (52%), Positives = 228/340 (66%), Gaps = 8/340 (2%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
L +AVIPGDGIG+EVVAEGLKVL + V E KE+ FGA Y TGETL D ++
Sbjct: 5 LNLAVIPGDGIGQEVVAEGLKVLSAVLPQD-VKLETKEFDFGARRYHATGETLTDADLDA 63
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
K DAI GAIGDP V G+LERG LLK+RF D +VNLRP KL TPL G+ +ID
Sbjct: 64 LKAHDAILLGAIGDPSVPSGVLERGFLLKLRFAFDHHVNLRPSKLLPGVATPLAGQPEID 123
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
V VRE TEG Y G GG +RKGT E+A + +NT +GVERV+R AF A+ RKK+TL
Sbjct: 124 FVVVREGTEGPYTGNGGTIRKGTEHEVATEVSVNTAYGVERVVRDAFARAQARPRKKLTL 183
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
V K NVLT+A LW +F +V+ EY ++ TD+ +VDA + ++ P FDV+VT N+FGD
Sbjct: 184 VHKNNVLTFAGHLWTNIFNKVAAEYPEVTTDYLHVDAATIFLVTDPARFDVIVTDNLFGD 243
Query: 242 ILTDLGAEIVGGLGIAASGNINP--ETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
I+TDL A + GG+G+AASGNINP + MFEPVHGSAPDIAG+ A+P A +LS +++L
Sbjct: 244 IITDLAAAVSGGIGVAASGNINPSGDFPSMFEPVHGSAPDIAGQGKADPTATVLSVALLL 303
Query: 300 EYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGD 339
+L E E+ IE+AV + E P + T E+GD
Sbjct: 304 RHLGYEDEAARIEDAVSADLGERGDLPARS----TSEIGD 339
>sp|P94631|LEU3_CORGL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1694761|emb|CAA70767.1| (Y09578)
3-isopropylmalate dehydrogenase [Corynebacterium
glutamicum]
Length = 340
Score = 321 bits (814), Expect = 8e-87
Identities = 180/348 (51%), Positives = 230/348 (65%), Gaps = 13/348 (3%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AVI GDGIG EV AE LKVL + + E +Y GA YLK GE L D +
Sbjct: 1 MKLAVIGGDGIGPEVTAEALKVLNAVRD----DIETTDYDLGARRYLKNGELLTDEDLAS 56
Query: 63 FKKFDAIYFGAIGDP-RVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKI 121
++ DAI GAIG P V PGILERG+LLKMRF LD +VNLRP KLY +PL+ KI
Sbjct: 57 LREHDAILLGAIGAPGSVPPGILERGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKI 116
Query: 122 DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVT 181
D V VRE TEG Y G GG +R GTP EIA + +NTR+G ERVIR+AFE A +S RKK+T
Sbjct: 117 DFVVVREGTEGAYTGNGGAIRVGTPHEIANETSVNTRYGAERVIRYAFELA-QSRRKKLT 175
Query: 182 LVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFG 240
LV K NVL + LW+R EV++EY ++ D+ ++DA + ++ P FDV+VT N+FG
Sbjct: 176 LVHKTNVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLVTDPSRFDVIVTDNLFG 235
Query: 241 DILTDLGAEIVGGLGIAASGNINPETT--GMFEPVHGSAPDIAGKRIANPLAAILSASMM 298
DILTD + GG+G+AASGNI+ T MFEPVHGSAPDIAG+ IA+P AAILSA+M+
Sbjct: 236 DILTDEAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAML 295
Query: 299 LEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLE 346
L +L E + IE A+ +A D + + T EVGD +VK L+
Sbjct: 296 LRHLGDEDNAVRIETAIAADVAGR----DNSQPISTTEVGDRIVKALQ 339
>sp|O33117|LEU3_MYCLE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|11251545|pir||T45419 3-isopropylmalate
dehydrogenase [imported] - Mycobacterium leprae
>gi|2414552|emb|CAB16441.1| (Z99263) 3-isopropylmalate
dehydrogenase [Mycobacterium leprae]
Length = 336
Score = 303 bits (767), Expect = 3e-81
Identities = 163/346 (47%), Positives = 226/346 (65%), Gaps = 13/346 (3%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++A+I GDGIG EVVA+ +K+L ++ + Y GA Y TGE LP+ + E
Sbjct: 1 MKLAIIGGDGIGPEVVAQAVKIL----DVVLPGVQKTTYDLGARRYHTTGELLPESVLAE 56
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
++ DAI GA+GDP V G+LERG+LL++RFELD ++NLRP +LY + L GK ID
Sbjct: 57 LREHDAILLGAVGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVNSLLAGKPDID 116
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
V VRE TEG Y G GG +R GTP E+A + +NT FGV RV++ AFE A R RK +TL
Sbjct: 117 FVVVREGTEGPYTGTGGAIRVGTPNEVATEVSVNTAFGVRRVVQDAFERA-RQRRKHLTL 175
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
V K NVLTYA LW R+ EV ++Y D+E + ++DA + ++ P FDV+VT N+FGD
Sbjct: 176 VHKNNVLTYAGTLWCRIVQEVGEKYPDVEVVYQHIDAATIYLVTEPSRFDVIVTDNLFGD 235
Query: 242 ILTDLGAEIVGGLGIAASGNINPETT--GMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
I+TDL A + GG+ +AASGNI+ T MFEPVHGSAPDIAG+ IA+P AAI+S +++L
Sbjct: 236 IITDLAAAVCGGIALAASGNIDATRTNPSMFEPVHGSAPDIAGQGIADPTAAIMSLALLL 295
Query: 300 EYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
+L ++ + +++AV +A NG T EVG+ + L
Sbjct: 296 AHLGEDEPAARLDQAVASYLATRG-----NGRFSTGEVGERIAAAL 336
>sp|P95313|LEU3_MYCTU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431335|pir||F70854 probable leuB protein
- Mycobacterium tuberculosis (strain H37RV)
>gi|1813644|gb|AAC45174.1| (U78887) 3-isopropylmalate
dehydrogenase [Mycobacterium tuberculosis]
>gi|2791592|emb|CAA16080.1| (AL021287) leuB
[Mycobacterium tuberculosis]
Length = 336
Score = 300 bits (759), Expect = 2e-80
Identities = 165/346 (47%), Positives = 219/346 (62%), Gaps = 13/346 (3%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++A+I GDGIG EV AE +KVL + + Y GA + TGE LPD + E
Sbjct: 1 MKLAIIAGDGIGPEVTAEAVKVLDAVVP----GVQKTSYDLGARRFHATGEVLPDSVVAE 56
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
+ DAI GAIGDP V G+LERG+LL++RFELD ++NLRP +LY +PL G ID
Sbjct: 57 LRNHDAILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVASPLSGNPGID 116
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
V VRE TEG Y G GG +R GTP E+A + +NT FGV RV+ AFE A+R RK +TL
Sbjct: 117 FVVVREGTEGPYTGNGGAIRVGTPNEVATEVSVNTAFGVRRVVADAFERARRR-RKHLTL 175
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
V K NVLT+A LW R EV + Y D+E + +VDA + MI P FDV+VT N+FGD
Sbjct: 176 VHKTNVLTFAGGLWLRTVDEVGECYPDVEVAYQHVDAATIHMITDPGRFDVIVTDNLFGD 235
Query: 242 ILTDLGAEIVGGLGIAASGNINP--ETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
I+TDL A + GG+G+AASGNI+ MFEPVHGSAPDIAG+ IA+P AAI+S +++L
Sbjct: 236 IITDLAAAVCGGIGLAASGNIDATRANPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLL 295
Query: 300 EYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
+L + ++ AV+ +A + L T +VG+ + L
Sbjct: 296 SHLGEHDAAARVDRAVEAHLATRG-----SERLATSDVGERIAAAL 336
>sp|P94929|LEU3_MYCBO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1813642|gb|AAC45173.1| (U78886)
3-isopropylmalate dehydrogenase [Mycobacterium bovis]
Length = 336
Score = 298 bits (755), Expect = 6e-80
Identities = 165/346 (47%), Positives = 218/346 (62%), Gaps = 13/346 (3%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++A+I GDGIG EV AE +KVL + + Y GA + TGE LPD + E
Sbjct: 1 MKLAIIAGDGIGPEVTAEAVKVLDAVVP----GVQKTSYDLGARRFHATGEVLPDSVVAE 56
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
+ DAI GAIGDP V G+LERG+LL++RFELD ++NLRP +LY +PL G ID
Sbjct: 57 LRNHDAILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVASPLSGNPGID 116
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
V VRE TEG Y G GG +R GTP E+A + +NT FGV RV+ AFE A+R RK +TL
Sbjct: 117 FVVVREGTEGPYTGNGGAIRVGTPNEVATEVSVNTAFGVRRVVADAFERARRR-RKHLTL 175
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
V K NVLT A LW R EV + Y D+E + +VDA + MI P FDV+VT N+FGD
Sbjct: 176 VHKTNVLTLAGGLWLRTVDEVGECYPDVEVAYQHVDAATIHMITDPGRFDVIVTDNLFGD 235
Query: 242 ILTDLGAEIVGGLGIAASGNINP--ETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
I+TDL A + GG+G+AASGNI+ MFEPVHGSAPDIAG+ IA+P AAI+S +++L
Sbjct: 236 IITDLAAAVCGGIGLAASGNIDATRANPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLL 295
Query: 300 EYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
+L + ++ AV+ +A + L T +VG+ + L
Sbjct: 296 SHLGEHDAAARVDRAVEAHLATRG-----SERLATSDVGERIAAAL 336
>sp|Q58991|IDH_METJA ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCINATE DECARBOXYLASE)
(IDH) (NADP+-SPECIFIC ICDH) (IDP)
>gi|2826436|gb|AAB99614.1| (U67599) 3-isopropylmalate
dehydrogenase 2 (leuB2) [Methanococcus jannaschii]
Length = 347
Score = 283 bits (717), Expect = 2e-75
Identities = 159/354 (44%), Positives = 223/354 (62%), Gaps = 26/354 (7%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++ VI GDGIGKEV+ E +K+L +L E FE + G E K G LP+ IE+
Sbjct: 2 MKVCVIEGDGIGKEVIPEAIKILNELGE-----FEIIKGEAGLECLKKYGNALPEDTIEK 56
Query: 63 FKKFDAIYFGAIGDPRVKPGILE--RGILLKMRFELDLYVNLRPVKLYHPRLTPLKGK-- 118
K+ D I FGAI P KPG ++ + ++ +R LY N+RP+ + + L GK
Sbjct: 57 AKEADIILFGAITSP--KPGEVQNYKSPIITLRKMFHLYANVRPINNFG--IGQLIGKIA 112
Query: 119 -------NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEY 171
IDIV +RENTE LY G R+ + A+ E + TR G ER+IRFAFEY
Sbjct: 113 DYEFLNAKNIDIVIIRENTEDLYVG-----RERLENDTAIAERVITRKGSERIIRFAFEY 167
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFD 231
A ++ RKKV+ + KANVL L+ VF E+ + Y++E D Y VD+ AM +I+ PE FD
Sbjct: 168 AIKNNRKKVSCIHKANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFD 227
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
V+VT NMFGDIL+D + ++GGLG+A S NI + +FEPVHGSAPDIAGK IANP+A+
Sbjct: 228 VIVTTNMFGDILSDEASALIGGLGLAPSANIG-DDKALFEPVHGSAPDIAGKGIANPMAS 286
Query: 292 ILSASMMLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
ILS +M+ +Y+ +++ I EAVK + KVTPD+ G+LKT +VGD ++ ++
Sbjct: 287 ILSIAMLFDYIGEKEKGDLIREAVKYCLINKKVTPDLGGDLKTKDVGDEILNYI 340
>pir||C64499 isocitrate dehydrogenase (EC 1.1.1.-) - Methanococcus jannaschii
Length = 371
Score = 282 bits (714), Expect = 4e-75
Identities = 159/354 (44%), Positives = 222/354 (61%), Gaps = 26/354 (7%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++ VI GDGIGKEV+ E K+L +L E FE + G E K G LP+ IE+
Sbjct: 26 MKVCVIEGDGIGKEVIPEAXKILNELGE-----FEIIKGEAGLECLKKYGNALPEDTIEK 80
Query: 63 FKKFDAIYFGAIGDPRVKPGILE--RGILLKMRFELDLYVNLRPVKLYHPRLTPLKGK-- 118
K+ D I FGAI P KPG ++ + ++ +R LY N+RP+ + + L GK
Sbjct: 81 AKEADIILFGAITSP--KPGEVQNYKSPIITLRKMFHLYANVRPINNFG--IGQLIGKIA 136
Query: 119 -------NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEY 171
IDIV +RENTE LY G R+ + A+ E + TR G ER+IRFAFEY
Sbjct: 137 DYEFLNAKNIDIVIIRENTEDLYVG-----RERLENDTAIAERVITRKGSERIIRFAFEY 191
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFD 231
A ++ RKKV+ + KANVL L+ VF E+ + Y++E D Y VD+ AM +I+ PE FD
Sbjct: 192 AIKNNRKKVSCIHKANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFD 251
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
V+VT NMFGDIL+D + ++GGLG+A S NI + +FEPVHGSAPDIAGK IANP+A+
Sbjct: 252 VIVTTNMFGDILSDEASALIGGLGLAPSANIG-DDKALFEPVHGSAPDIAGKGIANPMAS 310
Query: 292 ILSASMMLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
ILS +M+ +Y+ +++ I EAVK + KVTPD+ G+LKT +VGD ++ ++
Sbjct: 311 ILSIAMLFDYIGEKEKGDLIREAVKYCLINKKVTPDLGGDLKTKDVGDEILNYI 364
>sp|P76251|TTUC_ECOLI PROBABLE TARTRATE DEHYDROGENASE (TDH) >gi|7431321|pir||H64940
tartrate dehydrogenase (EC 1.1.1.93) - Escherichia coli
(strain K-12) >gi|1736423|dbj|BAA15595.1| (D90823)
3-isopropylmalate dehydrogenase (EC 1.1.1.85) (b-IPM
dehydrogenase) (ImdH) (3-IPM-DH). [Escherichia coli]
>gi|1736433|dbj|BAA15604.1| (D90824) 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) (b-IPM dehydrogenase) (ImdH)
(3-IPM-DH). [Escherichia coli]
>gi|1788101|gb|AAC74870.1| (AE000274) putative tartrate
dehydrogenase [Escherichia coli K12]
Length = 361
Score = 282 bits (713), Expect = 5e-75
Identities = 159/355 (44%), Positives = 216/355 (60%), Gaps = 12/355 (3%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGA-EHYLKTGETLPDWAIE 61
+RIA IPGDGIGKEV+ EG++VL+ E + F++ + + E+Y G+ +PD E
Sbjct: 5 MRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPDDWHE 64
Query: 62 EFKKFDAIYFGAIGDPRVKPGILER-GILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
+ +FDAIYFGA+G P P + G LLK R E D YVNLRPV+L+ PL GK
Sbjct: 65 QLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAGKQP 124
Query: 121 IDIVF--VRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRK 178
DI F VRENTEG Y+ GG + +GT E+ +QE + TR GV+R++R+AFE A+ RK
Sbjct: 125 GDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQSRPRK 184
Query: 179 KVTLVDKANVLTYAHDLW-ERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVVTPN 237
+T K+N L + W ERV A ++ D ++D + + + PE FDVVV N
Sbjct: 185 TLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVVVASN 244
Query: 238 MFGDILTDLGAEIVGGLGIAASGNINPETT--GMFEPVHGSAPDIAGKRIANPLAAILSA 295
+FGDIL+DLG G +GIA S N+NPE T +FEPVHGSAPDI GK IANP+A I +
Sbjct: 245 LFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIATIWAG 304
Query: 296 SMMLEYL-----NLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
+MML++L ++ I A++ IA TPDM GN T +V D + K +
Sbjct: 305 AMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKII 359
>gi|10956836 3-isopropylmalate dehydrogenase [Sphingomonas aromaticivorans]
>gi|11251547|pir||T31128 3-isopropylmalate dehydrogenase
(EC 1.1.1.85) - Sphingomonas aromaticivorans plasmid
pNL1 >gi|3378269|gb|AAD03852.1| (AF079317)
3-isopropylmalate dehydrogenase [Sphingomonas
aromaticivorans]
Length = 355
Score = 268 bits (677), Expect = 9e-71
Identities = 149/335 (44%), Positives = 212/335 (62%), Gaps = 10/335 (2%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
IA IPGDGIG+EVV ++VL EF+ + + + YL+TG+ +PD ++ +
Sbjct: 6 IASIPGDGIGREVVPAAIEVLTAAAAKCGFVLEFRHFDWSCQTYLETGKMMPDDGLDRLR 65
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
DA++ GA+G P V I G+LL +R E + YVNLRPV+L +PL+ K DI
Sbjct: 66 DHDAVFLGAVGFPGVPDHISLWGLLLPIRREFEQYVNLRPVRLLPGIASPLRDKAPGDID 125
Query: 125 F--VRENTEGLYAGAGGFLRKGTPQ-EIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVT 181
F VREN+EG Y+ GG R GT + EI VQ+ I +R G +R++R+AF++A+RSGR+ VT
Sbjct: 126 FWVVRENSEGEYSQIGG--RTGTGENEIVVQQAIFSRRGTDRILRYAFDFARRSGRRHVT 183
Query: 182 LVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFG 240
K+N L ++ W+ FA + EY D+ D Y+VD +A + + SPE F+VVV N+FG
Sbjct: 184 SATKSNGLYHSMPFWDERFAAIGAEYPDVAADQYHVDILAARFVMSPERFNVVVGSNLFG 243
Query: 241 DILTDLGAEIVGGLGIAASGNINPET--TGMFEPVHGSAPDIAGKRIANPLAAILSASMM 298
DIL+DLG I G + +A S N+NPE MFEPVHGSAPDIAG+ IANP+ I S +MM
Sbjct: 244 DILSDLGPGITGTIAVAPSANLNPERRFPSMFEPVHGSAPDIAGQGIANPIGQIWSGAMM 303
Query: 299 LEYLNLEKESKWIEEAVKRAIAENKV--TPDMNGN 331
LE+L + + + A++ A+ + TPD+ GN
Sbjct: 304 LEHLGEAEPAALVMRALETALIAPDIARTPDLGGN 338
>sp|Q51945|TTUC_PSEPU TARTRATE DEHYDROGENASE (TDH) >gi|1084041|pir||S48640 tartrate
dehydrogenase (EC 1.1.1.93) - Pseudomonas putida
>gi|458335|gb|AAA60327.1| (U05986) tartrate
dehydrogenase [Pseudomonas putida]
Length = 365
Score = 264 bits (667), Expect = 1e-69
Identities = 150/348 (43%), Positives = 214/348 (61%), Gaps = 13/348 (3%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGA-EHYLKTGETLPDWAIEE 62
RIA IPGDGIG EV+ EG++VL+ ++ EF + + + ++YL+ G+ +PD E+
Sbjct: 7 RIAAIPGDGIGLEVLPEGIRVLEAAALKHGLALEFDTFEWASCDYYLQHGKMMPDDWAEQ 66
Query: 63 FKKFDAIYFGAIGDP-RVKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKGKN 119
K++DAIYFGA+ P +V I G LLK R E D YVN+RPV+L+ P +
Sbjct: 67 LKQYDAIYFGAVDWPDKVPDHISLWGSLLKFRREFDQYVNIRPVRLFPGVPCALANRKVG 126
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKK 179
ID V VRENTEG Y+ GG + + T EI +QE I TR GV+R++++AF+ A++ RK
Sbjct: 127 DIDFVVVRENTEGEYSSLGGIMFENTENEIVIQESIFTRRGVDRILKYAFDLAEKRERKH 186
Query: 180 VTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD-VVVTPN 237
VT K+N + + W++ ++ Y + D ++D + + + PE FD VVV N
Sbjct: 187 VTSATKSNGMAISMPYWDKRTEAMAAHYPHVSWDKQHIDILCARFVLQPERFDVVVVASN 246
Query: 238 MFGDILTDLGAEIVGGLGIAASGNINPETT--GMFEPVHGSAPDIAGKRIANPLAAILSA 295
+FGDIL+DLG G +GIA S N+NPE +FEPVHGSAPDI GK IANP+A I S
Sbjct: 247 LFGDILSDLGPACAGTIGIAPSANLNPERNFPSLFEPVHGSAPDIFGKNIANPIAMIWSG 306
Query: 296 SMMLEYL-----NLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVG 338
++MLE+L ++ + A++R IA+ VTPDM G L T +VG
Sbjct: 307 ALMLEFLGQGDERYQRAHDDMLNAIERVIADGSVTPDMGGTLSTQQVG 354
>sp|P70792|TTC4_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)
>gi|984373|gb|AAB61628.1| (U32375) tartrate
dehydrogenase [Agrobacterium vitis]
Length = 358
Score = 262 bits (663), Expect = 4e-69
Identities = 145/344 (42%), Positives = 209/344 (60%), Gaps = 6/344 (1%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSR-VSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+IA IP DGIG EV+A GL+VL+ LE+ S S + + +G+++Y K G +P +E+
Sbjct: 5 KIAAIPADGIGPEVIAAGLQVLEALEKRSGDFSIHTETFDWGSDYYKKNGVMMPADGLEQ 64
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLK--GKNK 120
KKFDAI+FGA+G P V I G+ L + D Y N+RP K+ PL+ G
Sbjct: 65 LKKFDAIFFGAVGAPDVPDHITLWGLRLPICQGFDQYANVRPTKVLPGITPPLRNCGPGD 124
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+D V VREN+EG Y+G GG KG P+E+ + I TR GV R++R+AF+ A+ RK +
Sbjct: 125 LDWVIVRENSEGEYSGHGGRAHKGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPRKLL 184
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T+V K+N + +W+ + AEVS+E+ D+ D VDAM ++M P+S D +V N+
Sbjct: 185 TVVTKSNAQRHGMVMWDEIAAEVSKEFPDVTWDKMLVDAMTVRMTLKPQSLDTIVATNLH 244
Query: 240 GDILTDLGAEIVGGLGIAASGNINPET--TGMFEPVHGSAPDIAGKRIANPLAAILSASM 297
DIL+DL + G LG+A + NI+PE MFEP+HGSA DI GK IANP+A +A+
Sbjct: 245 ADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPVATFWTAAQ 304
Query: 298 MLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
MLE+L + + + AV+R +TPD+ G T +V D V
Sbjct: 305 MLEHLGEKDAATRLMSAVERVTEAGILTPDVGGTANTQQVTDAV 348
>sp|P70787|TTC2_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC (TDH)
>gi|984368|gb|AAB61623.1| (U32375) tartrate
dehydrogenase [Agrobacterium vitis]
Length = 364
Score = 262 bits (662), Expect = 5e-69
Identities = 145/344 (42%), Positives = 209/344 (60%), Gaps = 6/344 (1%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSR-VSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+IA IP DGIG EV+A GL+VL+ LE+ S S + + +G+++Y K G +P +E+
Sbjct: 5 KIAAIPADGIGPEVIAAGLQVLEALEKRSGDFSIHTETFDWGSDYYKKNGVMMPADGLEQ 64
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLK--GKNK 120
KKFDAI+FGA+G P V I G+ L + D Y N+RP K+ PL+ G
Sbjct: 65 LKKFDAIFFGAVGAPDVPDHITLWGLRLPICQGFDQYANVRPTKVLPGITPPLRNCGPGD 124
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+D V VREN+EG Y+G GG KG P+E+ + I TR GV R++R+AF+ A+ RK +
Sbjct: 125 LDWVIVRENSEGEYSGHGGRAHKGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPRKLL 184
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T+V K+N + +W+ + AEVS+E+ D+ D VDAM ++M P+S D +V N+
Sbjct: 185 TVVTKSNAQRHGMVMWDEIAAEVSKEFPDVTWDKMLVDAMTVRMTLKPQSLDTIVATNLH 244
Query: 240 GDILTDLGAEIVGGLGIAASGNINPET--TGMFEPVHGSAPDIAGKRIANPLAAILSASM 297
DIL+DL + G LG+A + NI+PE MFEP+HGSA DI GK IANP+A +A+
Sbjct: 245 ADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPVATFWTAAQ 304
Query: 298 MLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
MLE+L + + + AV+R +TPD+ G T +V D V
Sbjct: 305 MLEHLGEKDAATRLMSAVERVTEAGILTPDVGGTADTQQVTDAV 348
>gi|11498236 3-isopropylmalate dehydrogenase (leuB) [Archaeoglobus fulgidus]
>gi|6685585|sp|O29627|LEU3_ARCFU 3-ISOPROPYLMALATE
DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH) (3-IPM-DH)
>gi|7431327|pir||D69328 3-isopropylmalate dehydrogenase
(leuB) homolog - Archaeoglobus fulgidus
>gi|2649991|gb|AAB90611.1| (AE001061) 3-isopropylmalate
dehydrogenase (leuB) [Archaeoglobus fulgidus]
Length = 326
Score = 262 bits (662), Expect = 5e-69
Identities = 154/345 (44%), Positives = 211/345 (60%), Gaps = 23/345 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
+I VIPGDGIGKEV+ + +L+KL+ + FE+ Y G E K G+ LPD +E
Sbjct: 3 KIVVIPGDGIGKEVMEAAMLILEKLD----LPFEYSYYDAGDEALEKYGKALPDETLEAC 58
Query: 64 KKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+K DA+ FGA G+ +++++R EL + N+RP K G +DI
Sbjct: 59 RKSDAVLFGAAGETAAD-------VIVRLRRELGTFANVRPAKAIEGIECLYPG---LDI 108
Query: 124 VFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
V VRENTE LY G GF ++ + TR ER+ R+AFE AKR GRKKVT
Sbjct: 109 VVVRENTECLYMGFEFGF------GDVTEAIRVITREASERIARYAFELAKREGRKKVTA 162
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
+ KANV+ L+ V EV+++Y +++ + YY+DA M ++ P FDV+VT NMFGD
Sbjct: 163 LHKANVMKKTCGLFRDVCREVAKDYPEIQYNDYYIDAACMYLVMDPFRFDVIVTTNMFGD 222
Query: 242 ILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEY 301
I++DL A +VGGLG+A S N+ E T +FEPVHG+A DIAGK IANP A IL+A MML +
Sbjct: 223 IVSDLAAGLVGGLGLAPSANVG-ERTAIFEPVHGAAFDIAGKGIANPTAMILTACMMLRH 281
Query: 302 LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLE 346
+E+K +EEAV++ I E K TPD+ GNLKT E + V L+
Sbjct: 282 FGYVEEAKKVEEAVEKTIKEGKKTPDLGGNLKTMEFANEVASLLD 326
>sp|Q58130|LEU3_METJA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2826313|gb|AAB98716.1| (U67519)
3-isopropylmalate dehydrogenase 1 (leuB1) [Methanococcus
jannaschii]
Length = 333
Score = 260 bits (657), Expect = 2e-68
Identities = 158/349 (45%), Positives = 212/349 (60%), Gaps = 32/349 (9%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
+I VI GDGIGKEVV ++VL E + + FEF G E Y +TG+ LP+ IE
Sbjct: 3 KICVIEGDGIGKEVVPATIQVL----EATGLPFEFVYAEAGDEVYKRTGKALPEETIETA 58
Query: 64 KKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKG----KN 119
DA+ FGA G+ +++K+R LD Y N+RPVK Y KG +
Sbjct: 59 LDCDAVLFGAAGETAAD-------VIVKLRHILDTYANIRPVKAY-------KGVKCLRP 104
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA---KRSG 176
ID V VRENTEGLY G + +G I + + T ER+ RFAF A K+ G
Sbjct: 105 DIDYVIVRENTEGLYKGIEAEIDEG----ITIATRVITEKACERIFRFAFNLARERKKMG 160
Query: 177 RK-KVTLVDKANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVV 234
++ KVT KANVL L++++F +V++EYD ++ + YY+DAM M +I P+ FDVVV
Sbjct: 161 KEGKVTCAHKANVLKLTDGLFKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVV 220
Query: 235 TPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILS 294
T N+FGDIL+D A VGGLG+A S NI E G+FEPVHGSAPDIAGK+IANP A ILS
Sbjct: 221 TSNLFGDILSDGAAGTVGGLGLAPSANIGDEH-GLFEPVHGSAPDIAGKKIANPTATILS 279
Query: 295 ASMMLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
A +ML YL + + +E+A++ +A TPD+ GNL T+E+ + V K
Sbjct: 280 AVLMLRYLGEYEAADKVEKALEEVLALGLTTPDLGGNLNTFEMAEEVAK 328
>pir||H64389 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) - Methanococcus
jannaschii
Length = 337
Score = 260 bits (657), Expect = 2e-68
Identities = 158/349 (45%), Positives = 212/349 (60%), Gaps = 32/349 (9%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
+I VI GDGIGKEVV ++VL E + + FEF G E Y +TG+ LP+ IE
Sbjct: 7 KICVIEGDGIGKEVVPATIQVL----EATGLPFEFVYAEAGDEVYKRTGKALPEETIETA 62
Query: 64 KKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKG----KN 119
DA+ FGA G+ +++K+R LD Y N+RPVK Y KG +
Sbjct: 63 LDCDAVLFGAAGETAAD-------VIVKLRHILDTYANIRPVKAY-------KGVKCLRP 108
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA---KRSG 176
ID V VRENTEGLY G + +G I + + T ER+ RFAF A K+ G
Sbjct: 109 DIDYVIVRENTEGLYKGIEAEIDEG----ITIATRVITEKACERIFRFAFNLARERKKMG 164
Query: 177 RK-KVTLVDKANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVV 234
++ KVT KANVL L++++F +V++EYD ++ + YY+DAM M +I P+ FDVVV
Sbjct: 165 KEGKVTCAHKANVLKLTDGLFKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVV 224
Query: 235 TPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILS 294
T N+FGDIL+D A VGGLG+A S NI E G+FEPVHGSAPDIAGK+IANP A ILS
Sbjct: 225 TSNLFGDILSDGAAGTVGGLGLAPSANIGDEH-GLFEPVHGSAPDIAGKKIANPTATILS 283
Query: 295 ASMMLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
A +ML YL + + +E+A++ +A TPD+ GNL T+E+ + V K
Sbjct: 284 AVLMLRYLGEYEAADKVEKALEEVLALGLTTPDLGGNLNTFEMAEEVAK 332
>pir||A69113 isocitrate dehydrogenase - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2621228|gb|AAB84690.1| (AE000806)
isocitrate dehydrogenase [Methanobacterium
thermoautotrophicum]
Length = 331
Score = 256 bits (647), Expect = 3e-67
Identities = 152/351 (43%), Positives = 215/351 (60%), Gaps = 25/351 (7%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
RI VIPGDGIG EV+ L VL+ LE + FEF G E + + G+TLP+ ++
Sbjct: 3 RITVIPGDGIGVEVMEAALHVLQALE----IEFEFTHAEAGNECFRRCGDTLPEETLKLV 58
Query: 64 KKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKGKNKI 121
+K DA FGA+ PG ++ ++ +R ELDL+ NLRPVK P L P +
Sbjct: 59 RKADATLFGAV---TTVPG--QKSAIITLRRELDLFANLRPVKSLPGVPCLYP-----DL 108
Query: 122 DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVT 181
D V VRENTE LY G + +G AV + I TR R+ +FAF+YA++ G +KVT
Sbjct: 109 DFVIVRENTEDLYVGDEEYTPEG-----AVAKRIITRTASRRISQFAFQYAQKEGMQKVT 163
Query: 182 LVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFG 240
V KANVL ++ F +V+ EY +E + YYVDA AM +I P+ F +VT N+FG
Sbjct: 164 AVHKANVLKKTDGIFRDEFYKVASEYPQMEANDYYVDATAMYLITQPQEFQTIVTTNLFG 223
Query: 241 DILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLE 300
DIL+D A ++GGLG+A S NI E +FEPVHGSAP IAGK IANP A IL+ ++ML+
Sbjct: 224 DILSDEAAGLIGGLGLAPSANIG-EKNALFEPVHGSAPQIAGKNIANPTAMILTTTLMLK 282
Query: 301 YLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLEVVANE 351
+LN ++E++ IE+A+++ + +TPD+ G T E+ + + + E+V E
Sbjct: 283 HLNKKQEAQKIEKALQKTLMRGIMTPDLGGTASTMEMAEAIKE--EIVKGE 331
>sp|O34296|TTC3_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)
>gi|2305218|gb|AAB65748.1| (AF010263) tartrate
dehydrogenase [Agrobacterium vitis]
Length = 358
Score = 254 bits (642), Expect = 1e-66
Identities = 140/344 (40%), Positives = 206/344 (59%), Gaps = 6/344 (1%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSR-VSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+IA IP DGIG EV+A GL+VL+ LE+ S + + +G+++Y K G +P +++
Sbjct: 5 KIAAIPADGIGPEVIAAGLQVLEALEQRSGDFKIHTETFDWGSDYYKKHGVMMPADGLDK 64
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLK--GKNK 120
KKFDAI+FGA+G P V I G+ L + D Y N+RP K+ PL+ G
Sbjct: 65 LKKFDAIFFGAVGAPDVPDHITLWGLRLPICQGFDQYANVRPTKILPGITPPLRNCGPGD 124
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+D V VREN+EG Y+G GG +G P+E+ + I TR GV R++R+AF+ A+ RK +
Sbjct: 125 LDWVIVRENSEGEYSGHGGRAHRGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPRKLL 184
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T+V K+N + +W+ + AEV+ E+ D+ D VDAM ++M PE+ D +V N+
Sbjct: 185 TVVTKSNAQRHGMVMWDEIAAEVATEFPDVTWDKMLVDAMTVRMTLKPETLDTIVATNLH 244
Query: 240 GDILTDLGAEIVGGLGIAASGNINPET--TGMFEPVHGSAPDIAGKRIANPLAAILSASM 297
DIL+DL + G LG+A + NI+PE MFEP+HGSA DI GK IANP+A +A+
Sbjct: 245 ADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPIATFWTAAQ 304
Query: 298 MLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
MLE+L + + AV+R +TPD+ G T +V + V
Sbjct: 305 MLEHLGERDAAARLMSAVERVTEAGILTPDVGGTANTSQVTEAV 348
>sp|Q44471|TTC1_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC (TDH)
>gi|805292|gb|AAA68698.1| (U25634) putative tartrate
dehydrogenase; already transcribed in the absence of
tartrate and induced when trartrate is added; Method:
conceptual translation supplied by author [Agrobacterium
vitis] >gi|2305216|gb|AAB65747.1| (AF010262) tartrate
dehydrogenase [Agrobacterium vitis]
>gi|1585263|prf||2124372C ttuC gene [Agrobacterium
vitis]
Length = 364
Score = 254 bits (642), Expect = 1e-66
Identities = 140/344 (40%), Positives = 206/344 (59%), Gaps = 6/344 (1%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSR-VSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+IA IP DGIG EV+A GL+VL+ LE+ S + + +G+++Y K G +P +++
Sbjct: 5 KIAAIPADGIGPEVIAAGLQVLEALEQRSGDFKIHTETFDWGSDYYKKHGVMMPADGLDK 64
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLK--GKNK 120
KKFDAI+FGA+G P V I G+ L + D Y N+RP K+ PL+ G
Sbjct: 65 LKKFDAIFFGAVGAPDVPDHITLWGLRLPICQGFDQYANVRPTKILPGITPPLRNCGPGD 124
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+D V VREN+EG Y+G GG +G P+E+ + I TR GV R++R+AF+ A+ RK +
Sbjct: 125 LDWVIVRENSEGEYSGHGGRAHRGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPRKLL 184
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T+V K+N + +W+ + AEV+ E+ D+ D VDAM ++M PE+ D +V N+
Sbjct: 185 TVVTKSNAQRHGMVMWDEIAAEVATEFPDVTWDKMLVDAMTVRMTLKPETLDTIVATNLH 244
Query: 240 GDILTDLGAEIVGGLGIAASGNINPET--TGMFEPVHGSAPDIAGKRIANPLAAILSASM 297
DIL+DL + G LG+A + NI+PE MFEP+HGSA DI GK IANP+A +A+
Sbjct: 245 ADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPIATFWTAAQ 304
Query: 298 MLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
MLE+L + + AV+R +TPD+ G T +V + V
Sbjct: 305 MLEHLGERDAAARLMSAVERVTEAGILTPDVGGTANTRQVTEAV 348
>sp|O34295|TTC5_AGRVI PROBABLE TARTRATE DEHYDROGENASE TTUC' (TDH)
>gi|2305214|gb|AAB65746.1| (AF010261) tartrate
dehydrogenase [Agrobacterium vitis]
Length = 358
Score = 253 bits (640), Expect = 2e-66
Identities = 140/344 (40%), Positives = 206/344 (59%), Gaps = 6/344 (1%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSR-VSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+IA IP DGIG EV+A GL+VL+ LE+ S + + +G+++Y K G +P +++
Sbjct: 5 KIAAIPADGIGPEVIAAGLQVLEALEQRSGDFKIHTETFDWGSDYYKKHGVMMPADGLDK 64
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLK--GKNK 120
KKFDAI+FGA+G P V I G+ L + D Y N+RP K+ PL+ G
Sbjct: 65 LKKFDAIFFGAVGAPDVPDHITLWGLRLPICQGFDQYANVRPTKILPGITPPLRNCGPGD 124
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+D V VREN+EG Y+G GG +G P+E+ + I TR GV R++R+AF+ A+ RK +
Sbjct: 125 LDWVIVRENSEGEYSGHGGRAHRGLPEEVGTEVAIFTRVGVTRIMRYAFKLAQARPRKLL 184
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T+V K+N + +W+ + AEV+ E+ D+ D VDAM ++M PE+ D +V N+
Sbjct: 185 TVVTKSNAQRHGMVMWDEIAAEVATEFPDVTWDKMLVDAMTVRMTLKPETLDTIVATNLH 244
Query: 240 GDILTDLGAEIVGGLGIAASGNINPET--TGMFEPVHGSAPDIAGKRIANPLAAILSASM 297
DIL+DL + G LG+A + NI+PE MFEP+HGSA DI GK IANP+A +A+
Sbjct: 245 ADILSDLAGALAGSLGVAPTANIDPERRFPSMFEPIHGSAFDITGKGIANPIATFWTAAQ 304
Query: 298 MLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
MLE+L + + AV+R +TPD+ G T +V + V
Sbjct: 305 MLEHLGERDAAARLMGAVERVTEAGILTPDVGGTANTSQVTEAV 348
>sp|O66607|LEU3_AQUAE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431317|pir||C70322 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Aquifex aeolicus
>gi|2982949|gb|AAC06564.1| (AE000680) 3-isopropylmalate
dehydrogenase [Aquifex aeolicus]
Length = 364
Score = 250 bits (633), Expect = 1e-65
Identities = 151/362 (41%), Positives = 226/362 (61%), Gaps = 24/362 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K +IAV+ GDGIG E+V + L+VL K+ E+ V FE++E G E KTG+ LP+ ++
Sbjct: 3 KYKIAVLKGDGIGPEIVEQALRVLDKIGEIYGVEFEYREGLIGGEAIDKTGDPLPEETLK 62
Query: 62 EFKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTP 114
K+ DAI GA+G P+ +P E+G LL++R ELDLY NLRP K++ ++
Sbjct: 63 ICKESDAILLGAVGGPKWDNLPTDKRP---EKG-LLRIRKELDLYANLRPAKVWDALISS 118
Query: 115 LKGKNKI----DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFE 170
K ++ D++ +RE T G+Y G + + + A+ M T + R++R FE
Sbjct: 119 SPLKEEVVKGTDMIVIRELTSGIYYGEPRGIFEENGKRYAINTMKYTEDEIRRIVRKGFE 178
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPES 229
A++ RKK+T VDKANVL + LW ++ E + Y D+E +H Y+D AM+++R P S
Sbjct: 179 IARKR-RKKLTSVDKANVLEVSA-LWRQIVEEEKENYPDVELEHLYIDNCAMQLVRRPTS 236
Query: 230 FDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPL 289
FDV+VT N+FGDIL+D +VG LG+ S +I + ++EPVHGSAPDIAGK IANP+
Sbjct: 237 FDVIVTGNIFGDILSDEAGVVVGSLGMLPSASIG-DRYALYEPVHGSAPDIAGKGIANPI 295
Query: 290 AAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM--NGNLK--TYEVGDWVVKF 344
A ILSA+MML+Y N++K IE+A++ + + TPD+ G +K T E+ D +++
Sbjct: 296 ATILSAAMMLKYSFNMDKAHDLIEKAIEETLNQGYRTPDIYSEGCIKVGTKEITDKILEN 355
Query: 345 LE 346
LE
Sbjct: 356 LE 357
>sp|O27441|LEU3_METTH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431326|pir||F69051 3-isopropylmalate
dehydrogenase - Methanobacterium thermoautotrophicum
(strain Delta H) >gi|2622498|gb|AAB85865.1| (AE000901)
3-isopropylmalate dehydrogenase [Methanobacterium
thermoautotrophicum]
Length = 329
Score = 249 bits (630), Expect = 3e-65
Identities = 148/347 (42%), Positives = 207/347 (59%), Gaps = 25/347 (7%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
++IAVIPGDGIG EV+ L +L L+ + EF G +TG LP+ +E
Sbjct: 4 MKIAVIPGDGIGVEVMEAALHILNTLD----LDLEFIHADAGDACLKRTGTALPEETLEA 59
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKGKNK 120
+ A FGA G+ +++++R E DL+ NLRPVK P L P
Sbjct: 60 VGEARATLFGAAGESAAD-------VIVRLRREFDLFANLRPVKSLPGVPCLYP-----D 107
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+D V VRENTE LY G + +G AV + I TR R+ +FAF+YA++ G +KV
Sbjct: 108 LDFVIVRENTEDLYVGDEEYTPEG-----AVAKRIITRTASRRISQFAFQYAQKEGMQKV 162
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T V KANVL ++ F +V+ EY +E YYVDA AM +I P+ F +VT N+F
Sbjct: 163 TAVHKANVLKKTDGIFRDEFYKVASEYPQMEATDYYVDATAMYLITQPQEFQTIVTTNLF 222
Query: 240 GDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
GDIL+D A ++GGLG+A S NI E +FEPVHGSAP IAGK IANP A IL+ ++ML
Sbjct: 223 GDILSDEAAGLIGGLGLAPSANIG-EKNALFEPVHGSAPQIAGKNIANPTAMILTTTLML 281
Query: 300 EYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLE 346
++LN ++E++ IE+A+++ +AE VTPD+ G L T E+ + + LE
Sbjct: 282 KHLNKKQEAQKIEKALQKTLAEGLVTPDLGGKLGTMEMAAEIARHLE 328
>dbj|BAB04789.1| (AP001510) tartrate dehydrogenase [Bacillus halodurans]
Length = 353
Score = 248 bits (626), Expect = 9e-65
Identities = 132/347 (38%), Positives = 210/347 (60%), Gaps = 7/347 (2%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
K+ ++VIPGDG+GKE++ L+VL + E+ ++FE EYP+ E+YL+ GE +P+ +
Sbjct: 3 KITLSVIPGDGVGKEIMPHALRVLDAVAEVHGGLTFEKVEYPWSCEYYLEHGEMMPEDGL 62
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILER-GILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN 119
+ + DA++ GA+G+P++ P + G+L+ +R +N+R K P+K N
Sbjct: 63 VQLTESDAVFLGAVGNPQLVPDHVSLWGLLIAIRRGFQQSLNIRHAKQIRGIDPPIKHAN 122
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKK 179
+ D++ VREN+EG Y+ GG + +G E+ VQ I T+ +R I +AF+ A G K+
Sbjct: 123 QFDLLVVRENSEGEYSEVGGRIHQGD-DEVVVQNAIFTKKATKRAIDYAFQLAATRG-KR 180
Query: 180 VTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNM 238
VT K+N + ++ W+ VF EV ++Y D+E ++DA+ ++ P FDV+V N+
Sbjct: 181 VTSATKSNGIVFSMPFWDNVFQEVGKQYPDIEQQSVHIDALTAFLVTKPAEFDVIVASNL 240
Query: 239 FGDILTDLGAEIVGGLGIAASGNINPETT--GMFEPVHGSAPDIAGKRIANPLAAILSAS 296
FGDILTD+ A I+G +GI + N+N E T MFEPVHGSAPDI GK +ANP+ I +
Sbjct: 241 FGDILTDVSAAIMGSIGIGPAANLNVEGTYPSMFEPVHGSAPDIVGKGMANPIGQIWTGK 300
Query: 297 MMLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
MML++ E+ K + + V+ TPD+ G T EV D +++
Sbjct: 301 MMLDHFGEEELGKTLLDIVEDVTEAGIKTPDIGGRATTGEVADAIIE 347
>sp|Q9WZ26|LEU3_THEMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431334|pir||B72363 3-isopropylmalate
dehydrogenase - Thermotoga maritima (strain MSB8)
>gi|4981071|gb|AAD35641.1|AE001730_9 (AE001730)
3-isopropylmalate dehydrogenase [Thermotoga maritima]
Length = 354
Score = 236 bits (596), Expect = 3e-61
Identities = 155/361 (42%), Positives = 219/361 (59%), Gaps = 23/361 (6%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
++IAV+PGDGIG EVV E LKVL+ +E+ + +FE G + + GE LP+ +
Sbjct: 1 MKIAVLPGDGIGPEVVREALKVLEVVEKKTGKTFEKVFGHIGGDAIDRFGEPLPEETKKI 60
Query: 63 FKKFDAIYFGAIGDPRVKPGILER----GILLKMRFELDLYVNLRPVKLYHP--RLTPLK 116
+ DAI+ G++G P+ E+ G LL +R L+LY N+RP+K+Y ++PLK
Sbjct: 61 CLEADAIFLGSVGGPKWDDLPPEKRPEIGGLLALRKMLNLYANIRPIKVYRSLVHVSPLK 120
Query: 117 GK---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAK 173
K + +D+V VRE + G+Y G +G +E MI R VER+ R AFE AK
Sbjct: 121 EKVIGSGVDLVTVRELSYGVYYGQP----RGLDEEKGFDTMIYDRKTVERIARTAFEIAK 176
Query: 174 RSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDV 232
RKKVT VDKANVL Y+ LW +V EV++EY D+E H YVD AM++I P FDV
Sbjct: 177 NR-RKKVTSVDKANVL-YSSMLWRKVVNEVAREYPDVELTHIYVDNAAMQLILKPSQFDV 234
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAI 292
++T NMFGDIL+D A + G LG+ S + + ++EP GSAPDIAGK IANP+A I
Sbjct: 235 ILTTNMFGDILSDESAALPGSLGLLPSASFGDK--NLYEPAGGSAPDIAGKNIANPIAQI 292
Query: 293 LSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN----LKTYEVGDWVVKFLEV 347
LS +MMLE+ + +E++ IE AV+ I E T D+ + + T ++GD + K LE
Sbjct: 293 LSLAMMLEHSFGMVEEARKIERAVELVIEEGYRTRDIAEDPEKAVSTSQMGDLICKKLEE 352
Query: 348 V 348
+
Sbjct: 353 I 353
>pdb|1IDM| 3-Isopropylmalate Dehydrogenase, Loop-Deleted Chimera
Length = 343
Score = 234 bits (591), Expect = 1e-60
Identities = 150/344 (43%), Positives = 211/344 (60%), Gaps = 25/344 (7%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKGK 118
++ +A+ G++G P+ G + E+G LL +R DL+ NLRP K++ RL+PLK +
Sbjct: 61 VEEAEAVLLGSVGGPKWGTGSVRPEQG-LLSLRKSQDLFANLRPAKVFPGLERLSPLKEE 119
Query: 119 --NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRFAFEYA 172
+D++ VRE T G+Y G P+ ++ E NT R+ VERV R AFE A
Sbjct: 120 IARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARVAFE-A 170
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
R RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RSP FD
Sbjct: 171 ARKRRKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARFD 229
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
VVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IANP AA
Sbjct: 230 VVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIANPTAA 288
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
ILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 289 ILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 331
>pdb|1XAC| Chimera Isopropylmalate Dehydrogenase Between Bacillus Subtilis
(M) And Thermus Thermophilus (T) From N-Terminal: 20% T
Middle 20% M Residual 60% T, Mutated At S82r. Low
Temperature (100k) Structure. >gi|1431784|pdb|1XAD|
Chimera Isopropylmalate Dehydrogenase Between Bacillus
Subtilis (M) And Thermus Thermophilus (T) From
N-Terminal: 20% T Middle 20% M Residual 60% T, Mutated
At S82r. Low Temperature (150k) Structure
Length = 345
Score = 233 bits (588), Expect = 2e-60
Identities = 151/349 (43%), Positives = 213/349 (60%), Gaps = 33/349 (9%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHPR--LT 113
++ +A+ G++G P+ ++P E+G LL +R +LDL+ NLRPVK++ +
Sbjct: 61 VEEAEAVLLGSVGGPKWDQNPRELRP---EKG-LLSIRKQLDLFANLRPVKVFESLSDAS 116
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRF 167
PLK + + +D V VRE T G+Y G P+ ++ E NT R+ VERV R
Sbjct: 117 PLKKEYIDNVDFVIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARV 168
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AFE A R RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RS
Sbjct: 169 AFE-AARKRRKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRS 226
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P FDVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IA
Sbjct: 227 PARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIA 285
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
NP AAILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 286 NPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>pdb|1GC8|A Chain A, The Crystal Structure Of Thermus Thermophilus 3-
Isopropylmalate Dehydrogenase Mutated At 172th From Ala
To Phe >gi|10835549|pdb|1GC8|B Chain B, The Crystal
Structure Of Thermus Thermophilus 3- Isopropylmalate
Dehydrogenase Mutated At 172th From Ala To Phe
Length = 345
Score = 233 bits (587), Expect = 3e-60
Identities = 149/349 (42%), Positives = 213/349 (60%), Gaps = 33/349 (9%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLT 113
++ +A+ G++G P+ ++P E G LL +R DL+ NLRP K++ RL+
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKIRP---ETG-LLSLRKSQDLFANLRPAKVFPGLERLS 116
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRF 167
PLK + +D++ VRE T G+Y G P+ ++ E NT R+ VERV R
Sbjct: 117 PLKEEIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARV 168
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AFE+A++ RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RS
Sbjct: 169 AFEFARKR-RKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRS 226
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P FDVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IA
Sbjct: 227 PARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIA 285
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
NP AAILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 286 NPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>pdb|1DR0|A Chain A, Structure Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd708 >gi|6980527|pdb|1DR0|B Chain B,
Structure Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd708
Length = 346
Score = 231 bits (583), Expect = 9e-60
Identities = 149/349 (42%), Positives = 212/349 (60%), Gaps = 33/349 (9%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLT 113
++ +A+ G++G P+ ++P E G LL +R DL+ NLRP K++ RL+
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKIRP---ETG-LLSLRKSQDLFANLRPAKVFPGLERLS 116
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRF 167
PLK + +D++ VRE T G+Y G P+ ++ E NT R+ VERV R
Sbjct: 117 PLKEEIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARV 168
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AFE A++ RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RS
Sbjct: 169 AFELARKR-RKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRS 226
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P FDVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IA
Sbjct: 227 PARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIA 285
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
NP AAILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 286 NPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>pdb|1DR8|A Chain A, Structure Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd177 >gi|6980529|pdb|1DR8|B Chain B,
Structure Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd177
Length = 344
Score = 231 bits (583), Expect = 9e-60
Identities = 149/349 (42%), Positives = 212/349 (60%), Gaps = 33/349 (9%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLT 113
++ +A+ G++G P+ ++P E G LL +R DL+ NLRP K++ RL+
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKIRP---ETG-LLSLRKSQDLFANLRPAKVFPGLERLS 116
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRF 167
PLK + +D++ VRE T G+Y G P+ ++ E NT R+ VERV R
Sbjct: 117 PLKEEIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARV 168
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AFE A++ RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RS
Sbjct: 169 AFELARKR-RKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRS 226
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P FDVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IA
Sbjct: 227 PARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIA 285
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
NP AAILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 286 NPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>pdb|1DPZ|A Chain A, Stucture Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd711 >gi|6980659|pdb|1DPZ|B Chain B,
Stucture Of Modified 3-Isopropylmalate Dehydrogenase At
The C-Terminus, Hd711
Length = 349
Score = 231 bits (583), Expect = 9e-60
Identities = 149/349 (42%), Positives = 212/349 (60%), Gaps = 33/349 (9%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLT 113
++ +A+ G++G P+ ++P E G LL +R DL+ NLRP K++ RL+
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKIRP---ETG-LLSLRKSQDLFANLRPAKVFPGLERLS 116
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRF 167
PLK + +D++ VRE T G+Y G P+ ++ E NT R+ VERV R
Sbjct: 117 PLKEEIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARV 168
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AFE A++ RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RS
Sbjct: 169 AFELARKR-RKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRS 226
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P FDVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IA
Sbjct: 227 PARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIA 285
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
NP AAILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 286 NPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>pdb|1G2U|A Chain A, The Structure Of The Mutant, A172v, Of 3-Isopropylmalate
Dehydrogenase From Thermus Thermophilus Hb8 : Its
Thermostability And Structure
Length = 345
Score = 231 bits (583), Expect = 9e-60
Identities = 149/349 (42%), Positives = 212/349 (60%), Gaps = 33/349 (9%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLT 113
++ +A+ G++G P+ ++P E G LL +R DL+ NLRP K++ RL+
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKIRP---ETG-LLSLRKSQDLFANLRPAKVFPGLERLS 116
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRF 167
PLK + +D++ VRE T G+Y G P+ ++ E NT R+ VERV R
Sbjct: 117 PLKEEIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARV 168
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AFE A++ RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RS
Sbjct: 169 AFEVARKR-RKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRS 226
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P FDVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IA
Sbjct: 227 PARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIA 285
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
NP AAILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 286 NPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>pir||S41223 3-isopropylmalate dehydrogenase - Thermus aquaticus
>gi|7427675|pir||DETWIT 3-isopropylmalate dehydrogenase
(EC 1.1.1.85) - Thermus aquaticus >gi|1431780|pdb|1XAA|
3-Isopropylmalate Dehydrogenase, Low Temperature (100k)
Structure >gi|1431782|pdb|1XAB| 3-Isopropylmalate
Dehydrogenase, Low Temperature (150k) Structure
>gi|809121|pdb|1HEX| 3-Isopropylmalate Dehydrogenase
(E.C.1.1.1.85) Complexed With Beta-Nicotinamide Adenine
Dinucleotide, Oxidized (Nad+) >gi|1942477|pdb|1OSI|A
Chain A, Structure Of 3-Isopropylmalate Dehydrogenase
>gi|1942478|pdb|1OSI|B Chain B, Structure Of
3-Isopropylmalate Dehydrogenase >gi|1942479|pdb|1OSI|C
Chain C, Structure Of 3-Isopropylmalate Dehydrogenase
>gi|1942480|pdb|1OSI|D Chain D, Structure Of
3-Isopropylmalate Dehydrogenase
Length = 345
Score = 231 bits (583), Expect = 9e-60
Identities = 150/349 (42%), Positives = 211/349 (59%), Gaps = 33/349 (9%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLT 113
++ +A+ G++G P+ ++P E G LL +R DL+ NLRP K++ RL+
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKIRP---ETG-LLSLRKSQDLFANLRPAKVFPGLERLS 116
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRF 167
PLK + +D++ VRE T G+Y G P+ ++ E NT R+ VERV R
Sbjct: 117 PLKEEIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARV 168
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AFE A R RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RS
Sbjct: 169 AFE-AARKRRKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRS 226
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P FDVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IA
Sbjct: 227 PARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIA 285
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
NP AAILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 286 NPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>pdb|1OSJ|A Chain A, Structure Of 3-Isopropylmalate Dehydrogenase
>gi|1942482|pdb|1OSJ|B Chain B, Structure Of
3-Isopropylmalate Dehydrogenase
Length = 345
Score = 231 bits (583), Expect = 9e-60
Identities = 149/349 (42%), Positives = 212/349 (60%), Gaps = 33/349 (9%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLT 113
++ +A+ G++G P+ ++P E G LL +R DL+ NLRP K++ RL+
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKIRP---ETG-LLSLRKSQDLFANLRPAKVFPGLERLS 116
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRF 167
PLK + +D++ VRE T G+Y G P+ ++ E NT R+ VERV R
Sbjct: 117 PLKEEIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARV 168
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AFE A++ RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RS
Sbjct: 169 AFELARKR-RKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRS 226
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P FDVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IA
Sbjct: 227 PARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIA 285
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
NP AAILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 286 NPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>sp|P00351|LEU3_THETH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|443049|pdb|1IPD| 3-Isopropylmalate
Dehydrogenase (E.C.1.1.1.85) >gi|460072|gb|AAA16706.1|
(K01444) 3-isopropylmalate dehydrogenase [Thermus
thermophilus]
Length = 345
Score = 231 bits (582), Expect = 1e-59
Identities = 148/345 (42%), Positives = 208/345 (59%), Gaps = 25/345 (7%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR---VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKG 117
++ +A+ G++G P+ + I LL +R DL+ NLRP K++ RL+PLK
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKISPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKE 120
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRFAFEY 171
+ +D++ VRE T G+Y G P+ ++ E NT R+ VERV R AFE
Sbjct: 121 EIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARVAFE- 171
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A R RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RSP F
Sbjct: 172 AARKRRKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRSPARF 230
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
DVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IANP A
Sbjct: 231 DVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIANPTA 289
Query: 291 AILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
AILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 290 AILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>pdb|1GC9|A Chain A, The Crystal Structure Of Thermus Thermophilus 3-
Isopropylmalate Dehydrogenase Mutated At 172th From Ala
To Gly
Length = 345
Score = 230 bits (581), Expect = 2e-59
Identities = 149/349 (42%), Positives = 212/349 (60%), Gaps = 33/349 (9%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLT 113
++ +A+ G++G P+ ++P E G LL +R DL+ NLRP K++ RL+
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKIRP---ETG-LLSLRKSQDLFANLRPAKVFPGLERLS 116
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRF 167
PLK + +D++ VRE T G+Y G P+ ++ E NT R+ VERV R
Sbjct: 117 PLKEEIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARV 168
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AFE A++ RK V VDKANVL + W + EV + Y D+ +H YVDAMAM ++RS
Sbjct: 169 AFEGARKR-RKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAMAMHLVRS 226
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P FDVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IA
Sbjct: 227 PARFDVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIA 285
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
NP AAILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 286 NPTAAILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>sp|P31958|LEU3_CLOPA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|144842|gb|AAC41394.1| (L06666)
isopropylmalate dehydrogenase [Clostridium pasteurianum]
Length = 357
Score = 229 bits (579), Expect = 3e-59
Identities = 147/361 (40%), Positives = 215/361 (58%), Gaps = 23/361 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
+ +IAVIPGDGIG ++V E +K++ K+ E F F E G + GE LP I+
Sbjct: 3 EFKIAVIPGDGIGPDIVREAVKIMTKVGEKYDTKFNFVEVKAGGDAIDAYGEPLPKETID 62
Query: 62 EFKKFDAIYFGAIGDPR---VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPLK 116
K A+ GA+G P+ ++ LL +R L LY NLRP K+Y+ +PLK
Sbjct: 63 VCKSSAAVLLGAVGGPKWDNLEGSKRPERALLGLRGALGLYANLRPAKVYNVLKSASPLK 122
Query: 117 GK---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFGVERVIRFAFEYA 172
+ +D++ VRE G+Y G +GT + V+ +T ++ V+ V R A
Sbjct: 123 NEIIDEGVDLLVVRELIGGIYFGD-----RGTKEVNGVETAFDTEKYNVDEVKRIAHSAF 177
Query: 173 KRS--GRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPES 229
K + RKKVT VDKANVL A LW + EVS+EY ++E H YVD AM+++R P
Sbjct: 178 KAAMKRRKKVTSVDKANVLD-ASRLWRKTVNEVSKEYPEVELSHLYVDNTAMQLVRKPSQ 236
Query: 230 FDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPL 289
FDV++T N+FGDIL+D + I G +G+ AS +I ++ GM+EP+HGSAPDIAG IANPL
Sbjct: 237 FDVILTNNIFGDILSDEASMITGSIGMLASSSIREDSFGMYEPIHGSAPDIAGLDIANPL 296
Query: 290 AAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPD--MNGNLK--TYEVGDWVVKF 344
A ILSA+M++EY L++ + ++ +E AV++ + E T D + G K T E+G+ V++
Sbjct: 297 AQILSAAMLMEYSLDMSEAARDVEAAVEKVLNEGYRTADIYIEGTKKVGTSEMGNLVLER 356
Query: 345 L 345
L
Sbjct: 357 L 357
>sp|P24098|LEU3_THEAQ 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|77580|pir||JX0173 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Thermus aquaticus
>gi|217178|dbj|BAA01542.1| (D10700)
threo-D-3-isopropylmalate dehydrogenase, alpha-2 subunit
[Thermus aquaticus]
Length = 344
Score = 229 bits (578), Expect = 4e-59
Identities = 148/356 (41%), Positives = 212/356 (58%), Gaps = 25/356 (7%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+R+AV+PGDGIG EV L+VLK L+E + ++ +PFG GE P+ +
Sbjct: 1 MRVAVLPGDGIGPEVTEAALRVLKALDEREGLGLTYETFPFGGAAIDGYGEPFPEVTRKG 60
Query: 63 FKKFDAIYFGAIGDPR---VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKG 117
+ +A+ G++G P+ + I LL +R DL+ NLRP K++ RL+PLK
Sbjct: 61 VEAAEAVLLGSVGGPKWDALPRKIRPESGLLALRKSQDLFANLRPAKVFPGLERLSPLKE 120
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRFAFEY 171
+ +D++ VRE T G+Y G P+ ++ E NT R+ VERV + AFE
Sbjct: 121 EIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVAKVAFE- 171
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A R R+ +T VDKANVL + W + EV + Y D+ DH YVDAMAM ++++P F
Sbjct: 172 AARKRRRHLTSVDKANVLEVG-EFWRKTVEEVHKGYPDVALDHQYVDAMAMHLVKNPARF 230
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
DVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IANP A
Sbjct: 231 DVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIANPTA 289
Query: 291 AILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
AILSA+MMLE+ L + ++ +E AV +A+ E PD+ G+ T + V++ L
Sbjct: 290 AILSAAMMLEHAFGLVELARRVEAAVAKALRETP-PPDLGGSAGTQAFTEEVLRHL 344
>pir||A26522 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Bacillus subtilis
>gi|580891|emb|CAA68432.1| (Y00353) 3-isopropylmalate
dehydrogenase (AA 1 - 365) [Bacillus subtilis]
Length = 365
Score = 229 bits (578), Expect = 4e-59
Identities = 147/339 (43%), Positives = 203/339 (59%), Gaps = 14/339 (4%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
MK RIA++PGDGIG EV+ VLK + E FEF+ G + LP+ +
Sbjct: 1 MKKRIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETV 60
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILE----RGILLKMRFELDLYVNLRPVKLYHPRL--TP 114
K DAI GA+G P+ + E +G LL +R +LDL+ NLRPVK++ +P
Sbjct: 61 AACKNADAILLGAVGGPKWDQNLSELRPEKG-LLSIRKQLDLFANLRPVKVFESLSDRSP 119
Query: 115 LKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTP-QEIAVQEMINTRFGVERVIRFAFEY 171
LK + + +D V VRE T GLY G T ++ AV + R +ERVIR F+
Sbjct: 120 LKKEYIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVDTLFYKRTEIERVIREGFKM 179
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A + + KVT VDKANVL + LW V +V+QE+ D++ +H VD AM++I +P F
Sbjct: 180 AA-TRKGKVTSVDKANVLESSR-LWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQF 237
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
DVVVT NMFGDIL+D + + G LG+ S +++ +FEPVHGSAPDIAGK +ANP A
Sbjct: 238 DVVVTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFA 297
Query: 291 AILSASMMLE-YLNLEKESKWIEEAVKRAIAENKVTPDM 328
AILSA+M+L LE+E+K +E+AV + +A K T D+
Sbjct: 298 AILSAAMLLRTSFGLEEEAKAVEDAVNKVLASGKRTRDL 336
>sp|P05645|LEU3_BACSU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1770070|emb|CAA99532.1| (Z75208)
3-isopropylmalate dehydrogenase [Bacillus subtilis]
>gi|2635292|emb|CAB14787.1| (Z99118) 3-isopropylmalate
dehydrogenase [Bacillus subtilis]
Length = 365
Score = 229 bits (578), Expect = 4e-59
Identities = 147/339 (43%), Positives = 203/339 (59%), Gaps = 14/339 (4%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
MK RIA++PGDGIG EV+ VLK + E FEF+ G + LP+ +
Sbjct: 1 MKKRIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETV 60
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILE----RGILLKMRFELDLYVNLRPVKLYHPRL--TP 114
K DAI GA+G P+ + E +G LL +R +LDL+ NLRPVK++ +P
Sbjct: 61 AACKNADAILLGAVGGPKWDQNLSELRPEKG-LLSIRKQLDLFANLRPVKVFESLSDRSP 119
Query: 115 LKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTP-QEIAVQEMINTRFGVERVIRFAFEY 171
LK + + +D V VRE T GLY G T ++ AV + R +ERVIR F+
Sbjct: 120 LKKEYIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVDTLFYKRTEIERVIREGFKM 179
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A + + KVT VDKANVL + LW V +V+QE+ D++ +H VD AM++I +P F
Sbjct: 180 AA-ARKGKVTSVDKANVLESSR-LWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQF 237
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
DVVVT NMFGDIL+D + + G LG+ S +++ +FEPVHGSAPDIAGK +ANP A
Sbjct: 238 DVVVTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFA 297
Query: 291 AILSASMMLE-YLNLEKESKWIEEAVKRAIAENKVTPDM 328
AILSA+M+L LE+E+K +E+AV + +A K T D+
Sbjct: 298 AILSAAMLLRTSFGLEEEAKAVEDAVNKVLASGKRTRDL 336
>pdb|1WAL|A Chain A, 3-Isopropylmalate Dehydrogenase (Ipmdh) Mutant (M219a)from
Thermus Thermophilus
Length = 345
Score = 228 bits (576), Expect = 6e-59
Identities = 147/345 (42%), Positives = 207/345 (59%), Gaps = 25/345 (7%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+++AV+PGDGIG EV LKVL+ L+E + ++ +PFG GE P+ +
Sbjct: 1 MKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDAFGEPFPEPTRKG 60
Query: 63 FKKFDAIYFGAIGDPR---VKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKG 117
++ +A+ G++G P+ + I LL +R DL+ NLRP K++ RL+PLK
Sbjct: 61 VEEAEAVLLGSVGGPKWDGLPRKISPETGLLSLRKSQDLFANLRPAKVFPGLERLSPLKE 120
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFG---VERVIRFAFEY 171
+ +D++ VRE T G+Y G P+ ++ E NT R+ VERV R AFE
Sbjct: 121 EIARGVDVLIVRELTGGIYFGE--------PRGMSEAEAWNTERYSKPEVERVARVAFE- 171
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A R RK V VDKANVL + W + EV + Y D+ +H YVDA AM ++RSP F
Sbjct: 172 AARKRRKHVVSVDKANVLEVG-EFWRKTVEEVGRGYPDVALEHQYVDAAAMHLVRSPARF 230
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
DVVVT N+FGDIL+DL + + G LG+ S ++ T +FEPVHGSAPDIAGK IANP A
Sbjct: 231 DVVVTGNIFGDILSDLASVLPGSLGLLPSASLG-RGTPVFEPVHGSAPDIAGKGIANPTA 289
Query: 291 AILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKT 334
AILSA+MMLE+ L + ++ +E+AV +A+ E PD+ G+ T
Sbjct: 290 AILSAAMMLEHAFGLVELARKVEDAVAKALLETP-PPDLGGSAGT 333
>pir||G82070 3-isopropylmalate dehydrogenase VC2491 [imported] - Vibrio cholerae
(group O1 strain N16961) >gi|9657071|gb|AAF95633.1|
(AE004318) 3-isopropylmalate dehydrogenase [Vibrio
cholerae]
Length = 363
Score = 228 bits (575), Expect = 8e-59
Identities = 142/356 (39%), Positives = 207/356 (57%), Gaps = 16/356 (4%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
+IAV+PGDGIG EV+A+ KVL +E+ + F +E+ G G LP+ +
Sbjct: 7 KIAVLPGDGIGPEVMAQAHKVLDAIEQKHGIRFSREEHDVGGIAIDNHGCPLPESTLRAC 66
Query: 64 KKFDAIYFGAIGDPR---VKPGIL-ERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKG 117
++ DA+ FG++G P+ + P ERG LL +R L+ NLRP +++ +PL+
Sbjct: 67 EEADAVLFGSVGGPKWEHLPPNEQPERGALLPLRKHFQLFCNLRPAQIHQGLEAFSPLRA 126
Query: 118 K---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKR 174
DIV VRE T G+Y G E A + RF +ER+ R AFE A R
Sbjct: 127 DISARGFDIVVVRELTGGIYFGQPKGREGEGAHEKAFDTEVYHRFEIERIARIAFESA-R 185
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVV 233
RKKV +DKANVL + LW V +E+++EY D+ H Y+D M++I+ P FDV+
Sbjct: 186 LRRKKVCSIDKANVLQ-SSILWREVVSEIAKEYPDVSLSHMYIDNATMQLIKDPAQFDVM 244
Query: 234 VTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAIL 293
+ N+FGDIL+D A I G +G+ S ++N G++EP GSAPDIAGK IANP+A IL
Sbjct: 245 LCSNIFGDILSDECAMITGSMGMLPSASMNESKFGLYEPAGGSAPDIAGKNIANPVAQIL 304
Query: 294 SASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVKFL 345
SA++ML Y L E ++ IE AV +A+A ++T D+ G+ L T +GD + ++
Sbjct: 305 SAALMLRYSLGEEAAARDIENAVSQALAAGELTADLAGSKPALSTSAMGDKIASYI 360
>sp|P12010|LEU3_BACCO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|65953|pir||DEBSIC 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Bacillus coagulans
>gi|3212662|pdb|2AYQ|B Chain B, 3-Isopropylmalate
Dehydrogenase From The Moderate Facultative Thermophile,
Bacillus Coagulans >gi|3212661|pdb|2AYQ|A Chain A,
3-Isopropylmalate Dehydrogenase From The Moderate
Facultative Thermophile, Bacillus Coagulans
>gi|225041|prf||1207195A dehydrogenase,isopropylmalate
[Bacillus coagulans]
Length = 366
Score = 226 bits (571), Expect = 2e-58
Identities = 147/359 (40%), Positives = 215/359 (58%), Gaps = 18/359 (5%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
MK+++AV+PGDGIG EV+ ++VLK + + F+ G + G LP+ +
Sbjct: 1 MKMKLAVLPGDGIGPEVMDAAIRVLKTVLDNDGHEAVFENALIGGAAIDEAGTPLPEETL 60
Query: 61 EEFKKFDAIYFGAIGDPRV--KPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL--TP 114
+ ++ DAI GA+G P+ P L E+G LL +R E+ L+ NLRPVK Y L +P
Sbjct: 61 DICRRSDAILLGAVGGPKWDHNPASLRPEKG-LLGLRKEMGLFANLRPVKAYATLLNASP 119
Query: 115 LKGKN--KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA 172
LK + +D+V VRE T GLY G R+G + V + TR +ER+I AF+ A
Sbjct: 120 LKRERVENVDLVIVRELTGGLYFGRPSE-RRGPGENEVVDTLAYTREEIERIIEKAFQLA 178
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
+ RKK+ VDKANVL + +W + E +++Y D+E H VD+ +M++I +P FD
Sbjct: 179 QIR-RKKLASVDKANVLESSR-MWREIAEETAKKYPDVELSHMLVDSTSMQLIANPGQFD 236
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
V+VT NMFGDIL+D + I G LG+ S ++ + GM+EPVHGSAPDIAG+ ANPL
Sbjct: 237 VIVTENMFGDILSDEASVITGSLGMLPSASLRSDRFGMYEPVHGSAPDIAGQGKANPLGT 296
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGN-LKTYEVGDWVVKFL 345
+LSA++ML Y LEKE+ IE+AV + + T D+ NG + T E+ D +++ L
Sbjct: 297 VLSAALMLRYSFGLEKEAAAIEKAVDDVLQDGYCTGDLQVANGKVVSTIELTDRLIEKL 355
>gb|AAA22554.1| (M33099) 3-isopropylmalate dehydrogenase [Bacillus coagulans]
Length = 366
Score = 226 bits (571), Expect = 2e-58
Identities = 147/359 (40%), Positives = 215/359 (58%), Gaps = 18/359 (5%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
MK+++AV+PGDGIG EV+ ++VLK + + F+ G + G LP+ +
Sbjct: 1 MKMKLAVLPGDGIGPEVMDAAIRVLKTVLDNDGHEAVFENALIGGAAIDEAGTPLPEETL 60
Query: 61 EEFKKFDAIYFGAIGDPRV--KPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL--TP 114
+ ++ DAI GA+G P+ P L E+G LL +R E+ L+ NLRPVK Y L +P
Sbjct: 61 DICRRSDAILLGAVGGPKWDHNPASLRPEKG-LLGLRKEMGLFANLRPVKAYATLLNASP 119
Query: 115 LKGKN--KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA 172
LK + +D+V VRE T GLY G R+G + V + TR +ER+I AF+ A
Sbjct: 120 LKRERVENVDLVIVRELTGGLYFGRPSE-RRGPGENEVVDTLAYTREEIERIIEKAFQLA 178
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
+ RKK+ VDKANVL + +W + E +++Y D+E H VD+ +M++I +P FD
Sbjct: 179 QIR-RKKLASVDKANVLESSR-MWREIAEETAKKYPDVELSHMLVDSTSMQLIANPGQFD 236
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
V+VT NMFGDIL+D + I G LG+ S ++ + GM+EPVHGSAPDIAG+ ANPL
Sbjct: 237 VIVTENMFGDILSDEASVITGSLGMLPSASLRSDRFGMYEPVHGSAPDIAGQGKANPLGT 296
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGN-LKTYEVGDWVVKFL 345
+LSA++ML Y LEKE+ IE+AV + + T D+ NG + T E+ D +++ L
Sbjct: 297 VLSAALMLRYSFGLEKEAAAIEKAVDDVLQDGYCTGDLQVANGKVVSTIELTDRLIEKL 355
>pir||B81270 3-isopropylmalate dehydrogenase (EC 1.1.1.85) Cj1718c [imported] -
Campylobacter jejuni (strain NCTC 11168)
>gi|6969133|emb|CAB73704.1| (AL139079) 3-isopropylmalate
dehydrogenase [Campylobacter jejuni]
Length = 358
Score = 226 bits (571), Expect = 2e-58
Identities = 143/359 (39%), Positives = 215/359 (59%), Gaps = 26/359 (7%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
++AV+ GDGIG V+ E LK+L + + SFEF E G G L D ++
Sbjct: 5 KVAVLAGDGIGPLVMKEALKILTFIAQKYNFSFEFNEAKIGGASIDAYGVALSDETLKLC 64
Query: 64 KKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHP--RLTP 114
++ DAI FG++G P+ +P ER LL +R +L+ NLRP K+Y +P
Sbjct: 65 EQSDAILFGSVGGPKWDNLPIDQRP---ERASLLPLRKHFNLFANLRPCKIYESLTHASP 121
Query: 115 LKGK---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEY 171
LK + +DI+ VRE T G+Y G ++ +E A I T+ +ER+ R AFE
Sbjct: 122 LKNEIIQKGVDILCVRELTGGIYFG-----KQDLGKESAYDTEIYTKKEIERIARIAFES 176
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A R +KKV L+DKANVL + LW V A V+++Y D+ ++ YVD AM+++++P F
Sbjct: 177 A-RIRKKKVHLIDKANVLA-SSILWREVVANVAKDYQDINLEYMYVDNAAMQIVKNPSIF 234
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
DV++ N+FGDIL+D A I G LG+ +S ++N + G++EP GSAPDIA IANP+A
Sbjct: 235 DVMLCSNLFGDILSDELAAINGSLGLLSSASLNDKGFGLYEPAGGSAPDIAHLNIANPIA 294
Query: 291 AILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN--LKTYEVGDWVVKFLE 346
ILSA++ML+Y E+ ++ IE A+ A+A+ K+T D+N L T E+GD +++ L+
Sbjct: 295 QILSAALMLKYSFKEEQAAQDIENAISLALAQGKMTKDLNAKSYLNTDEMGDCILEILK 353
>sp|P42958|YCSA_BACSU HYPOTHETICAL 33.1 KD PROTEIN IN MTLD-SIPU INTERGENIC REGION
>gi|7431322|pir||A69765 tartrate dehydrogenase (EC
1.1.1.93) - Bacillus subtilis
>gi|2632701|emb|CAB12208.1| (Z99106) similar to
3-isopropylmalate dehydrogenase [Bacillus subtilis]
Length = 301
Score = 226 bits (569), Expect = 4e-58
Identities = 123/289 (42%), Positives = 183/289 (62%), Gaps = 8/289 (2%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
+ IA IPGDG+GKEVVA KVL E+ +SF F +P+ ++YL+ G+ +P+ I
Sbjct: 5 QFEIAAIPGDGVGKEVVAAAEKVLHTAAEVHGGLSFSFTAFPWSCDYYLEHGKMMPEDGI 64
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILER-GILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN 119
+F+A++ GA+G+P++ P + G+LLK+R EL+L +N+RP K +PL N
Sbjct: 65 HTLTQFEAVFLGAVGNPKLVPDHISLWGLLLKIRRELELSINMRPAKQMAGITSPLLHPN 124
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKK 179
D V +REN+EG Y+ GG + +G EIA+Q + TR ERV+RFAFE AK+ R
Sbjct: 125 DFDFVVIRENSEGEYSEVGGRIHRG-DDEIAIQNAVFTRKATERVMRFAFELAKKR-RTL 182
Query: 180 VTLVDKANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNM 238
VT K+N + +A W+ VF + + +Y +ET ++DA+A + PE+FDV+V +
Sbjct: 183 VTSATKSNGIYHAMPFWDEVFQQTAADYSGIETSSQHIDALAAFFVTRPETFDVIVASKL 242
Query: 239 FGDILTDLGAEIVGGLGIAASGNINP--ETTGMFEPVHGSAPDIAGKRI 285
FGDILTD+ + ++ +GIA +INP + MFEPVHGSAPDIAG+ +
Sbjct: 243 FGDILTDISSSLMESIGIAPP-DINPSGKYPSMFEPVHGSAPDIAGQAL 290
>pir||A64729 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Escherichia coli
>gi|1786260|gb|AAC73184.1| (AE000117) 3-isopropylmalate
dehydrogenase [Escherichia coli K12]
Length = 364
Score = 222 bits (559), Expect = 6e-57
Identities = 139/355 (39%), Positives = 200/355 (56%), Gaps = 16/355 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
IAV+PGDGIG EV+ + LKVL + + Y G G+ LP +E +
Sbjct: 8 IAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYDVGGAAIDNHGQPLPPATVEGCE 67
Query: 65 KFDAIYFGAIGDPR---VKPGIL-ERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKGK 118
+ DA+ FG++G P+ + P ERG LL +R L+ NLRP KLY PL+
Sbjct: 68 QADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHFKLFSNLRPAKLYQGLEAFCPLRAD 127
Query: 119 ---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRS 175
N DI+ VRE T G+Y G E A + RF +ER+ R AFE A++
Sbjct: 128 IAANGFDILCVRELTGGIYFGQPKGREGSGQYEKAFDTEVYHRFEIERIARIAFESARKR 187
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVV 234
R KVT +DKANVL + LW + E++ EY D+E H Y+D M++I+ P FDV++
Sbjct: 188 -RHKVTSIDKANVLQ-SSILWREIVNEIATEYPDVELAHMYIDNATMQLIKDPSQFDVLL 245
Query: 235 TPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILS 294
N+FGDIL+D A I G +G+ S ++N + G++EP GSAPDIAGK IANP+A ILS
Sbjct: 246 CSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILS 305
Query: 295 ASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGNLKTYEVGDWVVKFL 345
+++L Y L+ + + IE A+ RA+ E T D+ + T E+GD + +++
Sbjct: 306 LALLLRYSLDADDAACAIERAINRALEEGIRTGDLARGAAAVSTDEMGDIIARYV 360
>sp|P30125|LEU3_ECOLI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|628651|pir||S41197 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Escherichia coli
>gi|5542195|pdb|1CM7|A Chain A, 3-Isopropylmalate
Dehydrogenase From Escherichia Coli
>gi|5542196|pdb|1CM7|B Chain B, 3-Isopropylmalate
Dehydrogenase From Escherichia Coli
>gi|216491|dbj|BAA01342.1| (D10483) 3-isopropylmalate
dehydrogenase homolog(PIR:A24537) [Escherichia coli]
>gi|457440|dbj|BAA04537.1| (D17631) 3-isopropylmalate
dehydrogenase [Escherichia coli]
Length = 363
Score = 222 bits (559), Expect = 6e-57
Identities = 139/355 (39%), Positives = 200/355 (56%), Gaps = 16/355 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
IAV+PGDGIG EV+ + LKVL + + Y G G+ LP +E +
Sbjct: 7 IAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYDVGGAAIDNHGQPLPPATVEGCE 66
Query: 65 KFDAIYFGAIGDPR---VKPGIL-ERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKGK 118
+ DA+ FG++G P+ + P ERG LL +R L+ NLRP KLY PL+
Sbjct: 67 QADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHFKLFSNLRPAKLYQGLEAFCPLRAD 126
Query: 119 ---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRS 175
N DI+ VRE T G+Y G E A + RF +ER+ R AFE A++
Sbjct: 127 IAANGFDILCVRELTGGIYFGQPKGREGSGQYEKAFDTEVYHRFEIERIARIAFESARKR 186
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVV 234
R KVT +DKANVL + LW + E++ EY D+E H Y+D M++I+ P FDV++
Sbjct: 187 -RHKVTSIDKANVLQ-SSILWREIVNEIATEYPDVELAHMYIDNATMQLIKDPSQFDVLL 244
Query: 235 TPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILS 294
N+FGDIL+D A I G +G+ S ++N + G++EP GSAPDIAGK IANP+A ILS
Sbjct: 245 CSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILS 304
Query: 295 ASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGNLKTYEVGDWVVKFL 345
+++L Y L+ + + IE A+ RA+ E T D+ + T E+GD + +++
Sbjct: 305 LALLLRYSLDADDAACAIERAINRALEEGIRTGDLARGAAAVSTDEMGDIIARYV 359
>sp|P37412|LEU3_SALTY 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|5107574|pdb|1CNZ|A Chain A,
3-Isopropylmalate Dehydrogenase (Ipmdh) From Salmonella
Typhimurium >gi|5107575|pdb|1CNZ|B Chain B,
3-Isopropylmalate Dehydrogenase (Ipmdh) From Salmonella
Typhimurium >gi|732459|gb|AAB60185.1| (U20795)
3-isopropylmalate dehydrogenase [Salmonella typhimurium]
Length = 363
Score = 221 bits (558), Expect = 8e-57
Identities = 138/355 (38%), Positives = 200/355 (55%), Gaps = 16/355 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
IAV+PGDGIG EV+A+ LKV+ + + Y G G LP +E +
Sbjct: 7 IAVLPGDGIGPEVMAQALKVMDAVRSRFDMRITTSHYDVGGIAIDNHGHPLPKATVEGCE 66
Query: 65 KFDAIYFGAIGDPRVK----PGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKGK 118
+ DAI FG++G P+ + ERG LL +R L+ NLRP KLY PL+
Sbjct: 67 QADAILFGSVGGPKWENLPPESQPERGALLPLRKHFKLFSNLRPAKLYQGLEAFCPLRAD 126
Query: 119 ---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRS 175
N DI+ VRE T G+Y G E A + RF +ER+ R AFE A++
Sbjct: 127 IAANGFDILCVRELTGGIYFGQPKGREGSGQYEKAFDTEVYHRFEIERIARIAFESARKR 186
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVV 234
R+KVT +DKANVL + LW + +V++ Y D+E H Y+D M++I+ P FDV++
Sbjct: 187 -RRKVTSIDKANVLQ-SSILWREIVNDVAKTYPDVELAHMYIDNATMQLIKDPSQFDVLL 244
Query: 235 TPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILS 294
N+FGDIL+D A I G +G+ S ++N + G++EP GSAPDIAGK IANP+A ILS
Sbjct: 245 CSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILS 304
Query: 295 ASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGNLKTYEVGDWVVKFL 345
+++L Y L+ + IE+A+ RA+ E T D+ + T E+GD + +++
Sbjct: 305 LALLLRYSLDANDAATAIEQAINRALEEGVRTGDLARGAAAVSTDEMGDIIARYV 359
>sp|P43860|LEU3_HAEIN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1073789|pir||F64106 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Haemophilus influenzae
(strain Rd KW20) >gi|1574016|gb|AAC22648.1| (U32779)
3-isopropylmalate dehydrogenase (beta-IPM dehydrogenase)
(leuB) [Haemophilus influenzae Rd]
Length = 358
Score = 220 bits (556), Expect = 1e-56
Identities = 140/353 (39%), Positives = 202/353 (56%), Gaps = 16/353 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
IAV+ GDGIG EV+AE +KVL +++E F E+ G G LP ++
Sbjct: 6 IAVLAGDGIGPEVMAEAIKVLNRVQEKFGFKLNFNEFFVGGAAIEHCGYPLPAETLKGCD 65
Query: 65 KFDAIYFGAIGDPR---VKPGIL-ERGILLKMRFELDLYVNLRPVKLYH--PRLTPLKGK 118
+ DAI FG++G P+ + P ERG LL +R L+ NLRP LY + PL+
Sbjct: 66 QADAILFGSVGGPKWTNLPPDQQPERGALLPLRKHFKLFCNLRPATLYKGLEKFCPLRAD 125
Query: 119 ---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRS 175
D+V VRE T G+Y G Q A + ++ +ER+ R AFE A +
Sbjct: 126 IAAKGFDMVVVRELTGGIYFGQPKGREGDGVQTKAFDTEVYYKYEIERIARAAFEAAMKR 185
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVV 234
KKVT VDKANVL + LW E++++Y ++ +H Y+D M++I+SPESFDV++
Sbjct: 186 N-KKVTSVDKANVLQ-SSILWRETVTEMAKDYPEVTLEHIYIDNATMQLIKSPESFDVLL 243
Query: 235 TPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILS 294
N+FGDI++D A I G +G+ S ++N E G++EP GSAPDIAGK IANP+A ILS
Sbjct: 244 CSNIFGDIISDEAAMITGSMGMLPSASLNEEGFGLYEPAGGSAPDIAGKGIANPIAQILS 303
Query: 295 ASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVK 343
A+MML Y NL + + IE AV++ +A T D+ + + T E+G + +
Sbjct: 304 AAMMLRYSFNLNEAADAIESAVQKVLASGHRTADLADDSTPVSTAEMGTLITQ 356
>gb|AAG31390.1| (AF197452) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 364
Score = 219 bits (552), Expect = 4e-56
Identities = 138/362 (38%), Positives = 207/362 (57%), Gaps = 23/362 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RIAV+PGDGIG EV+ E K+L L++ + E KE+ G + G LP +
Sbjct: 4 KYRIAVLPGDGIGPEVMQEAYKILNILKDHFLLPLEMKEFNIGGIAIDQEGIALPKNTLL 63
Query: 62 EFKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHPR--L 112
+ DAI FG++G + +P ER LL +R +L+ NLRP KLY L
Sbjct: 64 GCENSDAILFGSVGGKKWDYLSIDKRP---ERAALLPLRKHFNLFSNLRPAKLYADLKYL 120
Query: 113 TPLKG---KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAF 169
+PL+ K+ DI+ VRE T G+Y G K E A + + + R+ AF
Sbjct: 121 SPLRSNIIKDGFDILCVRELTGGIYFGKPNGHSKKNDIEYAFDTEVYYDYEISRIAHLAF 180
Query: 170 EYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPE 228
+ A R+ +KKV +DK+NVL + LW+ + ++S+ Y D+ H Y+D M++I+ P
Sbjct: 181 QLA-RNRKKKVCSLDKSNVLK-SSILWKEIVEKISKNYPDVHLSHLYIDNAIMQIIKDPS 238
Query: 229 SFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANP 288
FDV++ N+FGDI++D A I G +G+ S ++N + G++EP GSAPDIAGK IANP
Sbjct: 239 QFDVILCSNLFGDIVSDECAAITGSIGMLPSASLNEKNFGLYEPAGGSAPDIAGKNIANP 298
Query: 289 LAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN----LKTYEVGDWVVK 343
+A ILS SM++ + +NL+K + I+E+V + + T D++ N LKT E+GD +
Sbjct: 299 IAQILSVSMLVRHGMNLKKIADKIDESVVSVLKQGYRTADISNNSHNYLKTSEMGDIIAN 358
Query: 344 FL 345
FL
Sbjct: 359 FL 360
>dbj|BAB06776.1| (AP001517) 3-isopropylmalate dehydrogenase [Bacillus halodurans]
Length = 363
Score = 218 bits (550), Expect = 7e-56
Identities = 137/338 (40%), Positives = 200/338 (58%), Gaps = 13/338 (3%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M+ +IAV+PGDGIG EV ++VL+ + + +F +K+ G + G LP+ +
Sbjct: 1 MEKQIAVLPGDGIGPEVTDAAIEVLQAVADRFGHTFSYKKALLGGCAIDEVGTPLPEETL 60
Query: 61 EEFKKFDAIYFGAIGDPR--VKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRLTPLK 116
+ + D I GA+G P+ PG L E+G LL +R L+L+ NLRPV +Y
Sbjct: 61 DVCRHADGILLGAVGGPKWDTLPGHLRPEKG-LLGLRKGLNLFANLRPVTVYDSLADAST 119
Query: 117 GKNKI----DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA 172
KN + D++ VRE T GLY G R E V ++ TR + R+IR AFE A
Sbjct: 120 LKNDVIDGVDLLIVRELTGGLYFGEPRERRGEGETEEVVDTLLYTRGEMRRIIRKAFELA 179
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
K VT VDKANVL + +W V EV+QE+ ++ +H VD AM++IR P+ FD
Sbjct: 180 MVRN-KHVTSVDKANVLESSR-MWREVANEVAQEFPEVTLEHMLVDNAAMQLIRRPKQFD 237
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
V+VT N+FGDIL+D + + G LG+ S ++ + G++EP+HGSAPDIAGK +ANPLA
Sbjct: 238 VLVTENLFGDILSDEASMVTGSLGMLPSASLTSDGPGLYEPIHGSAPDIAGKGVANPLAT 297
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM 328
I S +MML+Y L +E+K IE+A++ + + T D+
Sbjct: 298 IASCAMMLKYSFGLHEEAKTIEDAIEAVLKQGYRTADI 335
>gb|AAG31402.1| (AF197456) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 365
Score = 218 bits (549), Expect = 9e-56
Identities = 138/358 (38%), Positives = 202/358 (55%), Gaps = 18/358 (5%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
RIAV+PGDGIG EV+ E K+LK L+ + E KE+ G K G LP +
Sbjct: 6 RIAVLPGDGIGPEVMKEAYKILKILQNYFSLHIEIKEFNIGGIALDKEGVALPKDTLLGC 65
Query: 64 KKFDAIYFGAIGDPRVKPGILE----RGILLKMRFELDLYVNLRPVKLYHPR--LTPLKG 117
+ DAI FG++G + +E R LL +R +L+ NLRP KLY L+PL+
Sbjct: 66 ENSDAILFGSVGGKKWNNLPIEKRPERASLLPLRKHFNLFYNLRPAKLYSELRFLSPLRS 125
Query: 118 K----NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAK 173
K N DI+ +RE T G+Y G K E A + + + R+ AF+ A
Sbjct: 126 KTXIKNGFDILCIRELTGGIYFGEPRGRLKKNNIEYAFDTEVYYDYEINRIAHVAFQLA- 184
Query: 174 RSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDV 232
R+ + KV +DKANVL + LW + +VS+ Y D+ H Y+D M++I++P FD+
Sbjct: 185 RTRKNKVCSIDKANVLNSSL-LWREIVTKVSKNYPDVHLSHLYIDNATMQIIQNPNQFDI 243
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAI 292
++ N+FGDIL+D A I G +G+ S ++N + G++EP GSAPDIAGK IANP+A I
Sbjct: 244 LLCSNLFGDILSDECAIITGSIGMLPSASLNEKNFGLYEPAGGSAPDIAGKNIANPIAQI 303
Query: 293 LSASMMLEY-LNLEKESKWIEEAV----KRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
LS SM++ Y +NL+K + I+++V K+ ++ D N LKT E+GD + L
Sbjct: 304 LSLSMLVRYGMNLKKIANKIDQSVISVLKKGYRTFDISDDNNXFLKTNEMGDVIADAL 361
>sp|P48572|LEU3_BUCRP 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|899389|emb|CAA50616.1| (X71612)
isopropylmalate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 217 bits (548), Expect = 1e-55
Identities = 138/361 (38%), Positives = 205/361 (56%), Gaps = 22/361 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RIAV+PGDGIG E++ E K+L LE+ + E +E+ G + G LP+ +
Sbjct: 4 KFRIAVLPGDGIGPEIMREAYKILNILEKNFSLPLEIREFSVGGIAIDREGIALPEETLR 63
Query: 62 EFKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHP--RL 112
+ DAI FG++G + +P ER LL +R +L+ NLRP KLY L
Sbjct: 64 GCENSDAILFGSVGGKKWDYLPIESRP---ERASLLPLRKHFNLFSNLRPAKLYSDLKNL 120
Query: 113 TPLKG---KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAF 169
+PL+ +N DI+ VRE T G+Y G K E + I F ++R+ AF
Sbjct: 121 SPLRSNIVRNGFDILCVRELTGGIYFGKPKGRSKNENGEYSFDTEIYYDFEIKRIAHLAF 180
Query: 170 EYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPE 228
E A S R KV +DK+NVL + LW + EVS+ Y ++ H Y+D M++I++P
Sbjct: 181 ELAS-SRRCKVCSIDKSNVLA-SSILWREIVEEVSKNYPNIILSHLYIDNACMQIIKNPS 238
Query: 229 SFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANP 288
FDV++ N+FGDI++D A I G +G+ S ++N + G++EP GSAPDI GK IANP
Sbjct: 239 QFDVLLCSNIFGDIISDECAMITGSIGMLPSASLNSKKFGLYEPAGGSAPDIEGKNIANP 298
Query: 289 LAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVKF 344
+A ILS SM++ + +NL + + I+ +V RA+ T D++ +KT E+GD + KF
Sbjct: 299 IAQILSLSMLIRHSMNLNEIADKIDSSVYRALKAGYRTLDISDGKNYIKTNEMGDIIAKF 358
Query: 345 L 345
L
Sbjct: 359 L 359
>pir||D83255 3-isopropylmalate dehydrogenase PA3118 [imported] - Pseudomonas
aeruginosa (strain PAO1) >gi|3237310|gb|AAC23938.1|
(U93274) LeuB [Pseudomonas aeruginosa]
>gi|9949230|gb|AAG06506.1|AE004736_3 (AE004736)
3-isopropylmalate dehydrogenase [Pseudomonas aeruginosa]
Length = 360
Score = 217 bits (547), Expect = 2e-55
Identities = 142/363 (39%), Positives = 204/363 (56%), Gaps = 22/363 (6%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M +I V+PGDGIG E++AE +KVL+ + ++ FE E G K G L D +
Sbjct: 1 MSKQILVLPGDGIGPEIMAEAVKVLELANDRFQLGFELAEDVIGGAAIDKHGVPLADQTL 60
Query: 61 EEFKKFDAIYFGAIGDP---RVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKG 117
+ ++ DA+ GA+G P R++ I LLK+R +L L+ NLRP LY P+L
Sbjct: 61 QRARQADAVLLGAVGGPKWDRIERDIRPERGLLKIRSQLGLFANLRPAILY-PQLAAASS 119
Query: 118 KNK-----IDIVFVRENTEGLYAGAGG---FLRKGTPQEIAVQEMINTRFGVERVIRFAF 169
+DI+ VRE T G+Y G L G Q A + + + R+ R F
Sbjct: 120 LKPEVVAGLDILIVRELTGGIYFGQPREQRVLENGERQ--AYDTLPYSESEIRRIARVGF 177
Query: 170 EYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPE 228
+ A R ++ VDKANVL + LW V EV+++Y D+E H YVD AM+++R+P+
Sbjct: 178 DMA-RVRNNRLCSVDKANVLA-SSQLWREVVEEVAKDYPDVELSHMYVDNAAMQLVRAPK 235
Query: 229 SFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANP 288
FDV+VT NMFGDIL+D + + G +G+ S +++ GM+EP HGSAPDIAG+ IANP
Sbjct: 236 QFDVMVTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGIANP 295
Query: 289 LAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNG----NLKTYEVGDWVVK 343
LA ILS SMML Y + + IE+AV + + + T D+ + T E+GD VV
Sbjct: 296 LATILSVSMMLRYSFSQATAADAIEQAVSKVLDQGLRTGDIWSEGCRKVGTREMGDAVVA 355
Query: 344 FLE 346
L+
Sbjct: 356 ALK 358
>sp|Q51375|LEU3_PSEAE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1314267|gb|AAC45419.1| (U29655)
3-isopropylmalate dehydrogenase [Pseudomonas aeruginosa]
Length = 360
Score = 217 bits (546), Expect = 2e-55
Identities = 142/363 (39%), Positives = 203/363 (55%), Gaps = 22/363 (6%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M +I V+PGDGIG E++AE +KVL+ + ++ FE E G K G L D +
Sbjct: 1 MSKQILVLPGDGIGPEIMAEAVKVLELANDRFQLGFELAEDVIGGAAIDKHGVPLADQTL 60
Query: 61 EEFKKFDAIYFGAIGDP---RVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKG 117
+ ++ DA+ GA+G P R++ I LLK R +L L+ NLRP LY P+L
Sbjct: 61 QRARQADAVLLGAVGGPKWDRIERDIRPERGLLKFRSQLGLFANLRPAILY-PQLAAASS 119
Query: 118 KNK-----IDIVFVRENTEGLYAGAGG---FLRKGTPQEIAVQEMINTRFGVERVIRFAF 169
+DI+ VRE T G+Y G L G Q A + + + R+ R F
Sbjct: 120 LKPEVVAGLDILIVRELTGGIYFGQPREQRVLENGERQ--AYDTLPYSESEIRRIARVGF 177
Query: 170 EYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPE 228
+ A R ++ VDKANVL + LW V EV+++Y D+E H YVD AM+++R+P+
Sbjct: 178 DMA-RVRNNRLCSVDKANVLA-SSQLWREVVEEVAKDYPDVELSHMYVDNAAMQLVRAPK 235
Query: 229 SFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANP 288
FDV+VT NMFGDIL+D + + G +G+ S +++ GM+EP HGSAPDIAG+ IANP
Sbjct: 236 QFDVMVTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGIANP 295
Query: 289 LAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNG----NLKTYEVGDWVVK 343
LA ILS SMML Y + + IE+AV + + + T D+ + T E+GD VV
Sbjct: 296 LATILSVSMMLRYSFSQATAADAIEQAVSKVLDQGLRTGDIWSEGCRKVGTREMGDAVVA 355
Query: 344 FLE 346
L+
Sbjct: 356 ALK 358
>sp|P24015|LEU3_LEPIN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|149619|gb|AAA72088.1| (M59431)
3-isopropylmalate dehydrogenase [Leptospira
borgpetersenii]
Length = 358
Score = 216 bits (545), Expect = 3e-55
Identities = 140/358 (39%), Positives = 208/358 (57%), Gaps = 20/358 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+AV+ GDGIG EV+ + VLKK F+FKE G KTG LP ++ +
Sbjct: 4 VAVLSGDGIGPEVMEIAISVLKKALGAKVSEFQFKEGFVGGIAIDKTGHPLPPETLKLCE 63
Query: 65 KFDAIYFGAIGDPRVKP----GILERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
+ AI FG++G P+ + ERG LL +R DL+ NLRP +Y P L +P++
Sbjct: 64 ESSAILFGSVGGPKWETLPPEKQPERGALLPLRKHFDLFANLRPAIIY-PELKNASPVRS 122
Query: 118 K---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKR 174
N +DI+ +RE T G+Y G +E A M +R +ER+ + AF+ A R
Sbjct: 123 DIIGNGLDILILRELTGGIYFGQPKGREGSGQEEFAYDTMKYSRREIERIAKVAFQ-AAR 181
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY--DLETDHYYVDAMAMKMIRSPESFDV 232
KVT +DKANVLT + W+ V E+ ++ D++ +H YVD AM++I +P+ FDV
Sbjct: 182 KRNNKVTSIDKANVLTTSV-FWKEVVIELHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV 240
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAI 292
V+ NMFGDIL+D + I G +G+ S +++ G++EP GSAPDIAGK +ANP+A +
Sbjct: 241 VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQV 300
Query: 293 LSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM----NGNLKTYEVGDWVVKFL 345
LSA++ML Y ++E+E+ IE AV++ IA K T D+ + + T E+G + FL
Sbjct: 301 LSAALMLRYSFSMEEEANKIETAVRKTIASGKRTRDIAEVGSTIVGTKEIGQLIESFL 358
>sp|Q02143|LEU3_LACLA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|486725|pir||S35133 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Lactococcus lactis subsp.
lactis >gi|2565152|gb|AAB81914.1| (U92974) LeuB
[Lactococcus lactis]
Length = 345
Score = 216 bits (545), Expect = 3e-55
Identities = 131/354 (37%), Positives = 201/354 (56%), Gaps = 23/354 (6%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M +I + GDGIG E+++ GL VLK + + +E + FG K G LP+ +
Sbjct: 1 MSKKIVTLAGDGIGPEIMSAGLSVLKAVSKKIDFEYELEAKDFGGIAIDKHGHPLPEETL 60
Query: 61 EEFKKFDAIYFGAIGDPR-----VKPGILERGILLKMRFELDLYVNLRPVKLYHP--RLT 113
+ K DAI AIG P+ V+P E+G LL +R EL LY N+RP+K+Y +L+
Sbjct: 61 QAVKNADAILLAAIGHPKYNNAKVRP---EQG-LLALRKELGLYANVRPLKIYPALKKLS 116
Query: 114 PLKGKNKIDIVFVRENTEGLYAGAGGFL--RKGTPQEIAVQEMINTRFGVERVIRFAFEY 171
P++ +D + +RE T G+Y G + + + E + R++ FAF+
Sbjct: 117 PIRNVENVDFLVIRELTGGIYFGQHELADDKARDVNDYSADE-------IRRILHFAFKS 169
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A+ RK +T VDK NVL + LW ++ E++ EY D+ +H VD+ AM +I +P+ F
Sbjct: 170 AQSRPRKLLTSVDKQNVLATSK-LWRKMADEIADEYPDVRLEHQLVDSCAMLLITNPQQF 228
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
DV+VT N+FGDIL+D + + G LG+ S + ++EP+HGSAPDIAGK IANP++
Sbjct: 229 DVIVTENLFGDILSDEASSLAGSLGVMPSSSHGFNGLALYEPIHGSAPDIAGKGIANPVS 288
Query: 291 AILSASMML-EYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
ILS +MML E E + IE+AV + + +T D+ G T E+ + ++K
Sbjct: 289 MILSIAMMLRESFGQEDGAAMIEKAVTQTFTDGILTKDLGGTATTKEMTEAILK 342
>gb|AAG31387.1| (AF197451) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 216 bits (545), Expect = 3e-55
Identities = 138/361 (38%), Positives = 202/361 (55%), Gaps = 22/361 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RIA++PGDGIG EV+ E K+L L++ +S E +E+ G + G LP +
Sbjct: 4 KYRIAILPGDGIGPEVMREAYKILSILKDHFSLSLEIQEFNIGGSAIDQEGIALPKKTLL 63
Query: 62 EFKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHP--RL 112
+ DAI FG++G R +P ER LL +R +L+ NLRP KLY L
Sbjct: 64 GCQNSDAILFGSVGGKRWDHLPINERP---ERASLLPLRKHFNLFANLRPAKLYSELQHL 120
Query: 113 TPLKG---KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAF 169
+P++ KN DI+ VRE T G+Y G K E A + + + R+ AF
Sbjct: 121 SPIRSNIIKNGFDILCVRELTGGIYFGKPSDRVKKNNVEYAFDTEVYYDYEINRIAHLAF 180
Query: 170 EYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPE 228
+ A R+ + KV +DK+NVL + LW V +VS+ Y D+ H Y+D M++I++P
Sbjct: 181 QLA-RTRKFKVCSIDKSNVLN-SSILWREVVEKVSKMYPDVHLSHLYIDNATMQIIKNPN 238
Query: 229 SFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANP 288
FDV++ N+FGDI++D A I G +G+ S + N + G++EP GSAPDIAGK ANP
Sbjct: 239 QFDVLLCSNLFGDIISDECAVITGSIGMLPSASFNEKNFGLYEPAGGSAPDIAGKNTANP 298
Query: 289 LAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGNLKTYEVGDWVVKF 344
+A ILS SM++ Y + L K + I++AV ++ T D+ N LKT E+GD + F
Sbjct: 299 IAQILSLSMLVRYGMKLNKIADKIDDAVALSLKAGYRTADISNDNNFLKTNEMGDVIANF 358
Query: 345 L 345
L
Sbjct: 359 L 359
>gb|AAG31384.1| (AF197450) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 364
Score = 216 bits (544), Expect = 4e-55
Identities = 138/358 (38%), Positives = 210/358 (58%), Gaps = 19/358 (5%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
RIAV+PGDGIG EV+ E K+LK L+ +SFE +E+ G + G LP +
Sbjct: 6 RIAVLPGDGIGPEVMREAYKILKILKNNFLLSFEIEEFNVGGIAIDQEGLALPKKTLLGC 65
Query: 64 KKFDAIYFGAIGDPRVKPGILE----RGILLKMRFELDLYVNLRPVKLYHP--RLTPLKG 117
+K DAI FG++G + +E R LL +R +L+ NLRP K+Y L+PL+
Sbjct: 66 EKSDAILFGSVGGKKWDNFPIEERPERAALLPLRKHFNLFANLRPAKIYSELKHLSPLRS 125
Query: 118 ---KNKIDIVFVRENTEGLYAG--AGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA 172
++ DI+ +RE T G+Y G +G L K E A I + + R+ AF+ A
Sbjct: 126 NIVRDGFDILCIRELTGGIYFGQPSGRRLEKNNI-EYAFDTEIYYDYEINRIAHLAFQLA 184
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
+ S KV +DK+NVL + LW+ + +VS+ Y D++ H Y+D M++I++P FD
Sbjct: 185 Q-SRSHKVCSIDKSNVLN-SSILWKEIVQKVSKNYPDVDLSHLYIDNAIMQIIKNPNQFD 242
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
V++ PN+FGDI++D A I G +G+ S ++N + G++EP GSAPDIAGK IANP+A
Sbjct: 243 VLLCPNLFGDIISDECAIITGSIGMLPSASLNEKKFGLYEPAGGSAPDIAGKNIANPIAQ 302
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVKFL 345
ILS SM++ Y +NL+ + I+++V + + T D++ N LKT E+GD + L
Sbjct: 303 ILSLSMLVRYGMNLKDIADKIDKSVLSVLKKGYRTADISNNNNYLKTNEMGDVIANAL 360
>pir||S20606 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Salmonella
typhimurium >gi|48753|emb|CAA37456.1| (X53376) leuB
[Salmonella typhimurium]
Length = 359
Score = 215 bits (542), Expect = 6e-55
Identities = 137/355 (38%), Positives = 199/355 (55%), Gaps = 20/355 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
IAV+PGDGIG EV+A+ LKV+ + L + Y G +G LP +E +
Sbjct: 7 IAVLPGDGIGPEVMAQALKVMDAVRVL--ICVYHSHYDVGGIAIDSSGIPLPKATVEGCE 64
Query: 65 KFDAIYFGAIGDPRV------KPGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPLK 116
+ DAI FG++G P++ KP R LL +R L+ NLRP KLY PL+
Sbjct: 65 QADAILFGSVGGPKMGKFAPGKPAGARR--LLPLRKHFKLFSNLRPAKLYQGLEAFCPLR 122
Query: 117 GK---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAK 173
N DI+ VRE T G+Y G E A + RF +ER+ R AFE A+
Sbjct: 123 ADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEKAFDTEVYHRFEIERIARIAFESAR 182
Query: 174 RSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDV 232
+ R KVT +DKANVL + LW + +V++ Y D+E H Y+D M++I+ P FDV
Sbjct: 183 KR-RCKVTSIDKANVLQ-SSILWREIVNDVAKTYPDVELAHMYIDNATMQLIKDPSQFDV 240
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAI 292
++ N+FGDIL+D A I G +G+ S ++N + G++EP GSAPDIAGK IANP+A I
Sbjct: 241 LLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQI 300
Query: 293 LSASMMLEYLNLEKESKWIEEAVKRAIAENKVTPDM--NGNLKTYEVGDWVVKFL 345
LS +++L + + IE+A+ RA+ E T D+ + T E+GD + +++
Sbjct: 301 LSLALLLRTADANDAATAIEQAINRALEEGVRTGDLARGAAVSTDEMGDIIARYV 355
>gi|10954451 isopropylmalate dehydrogenase [Buchnera aphidicola]
>gi|3282733|gb|AAD12594.1| (AF041836) isopropylmalate
dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 214 bits (540), Expect = 1e-54
Identities = 138/362 (38%), Positives = 204/362 (56%), Gaps = 22/362 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RIAV+PGDGIG EV+ E K+L LE + E +E+ G + G LP+ +
Sbjct: 4 KFRIAVLPGDGIGPEVMREAYKILNILERKFFLKLELREFDIGGIAIDREGIALPEKTLR 63
Query: 62 EFKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHP--RL 112
+ DAI FG++G + +P ER LL +R +L+ NLRP KL L
Sbjct: 64 GCENSDAILFGSVGGKKWDYLPIESRP---ERASLLPLRKHFNLFSNLRPAKLRVDLKNL 120
Query: 113 TPLKG---KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAF 169
+PL+ ++ DI+ +RE T G+Y G K E A I F +ER+ AF
Sbjct: 121 SPLRSDIVRDGFDILCIRELTGGIYFGKPKGRFKNKNGEYAFDTEIYYDFEIERIANLAF 180
Query: 170 EYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPE 228
E A S R KV +DKANVL + LW + +VS+ Y D+ H Y+D M++I++P
Sbjct: 181 ELAS-SRRCKVCSIDKANVLE-SSVLWREIVKKVSKNYPDVHLSHLYIDNACMQIIKNPT 238
Query: 229 SFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANP 288
FD+++ N+FGDI++D A I G +G+ S ++N + G++EP GSAPDI GK IANP
Sbjct: 239 QFDILLCSNIFGDIISDECAMITGSIGMLPSASLNNQKFGLYEPAGGSAPDIEGKNIANP 298
Query: 289 LAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVKF 344
+A ILS SM++ Y +NL + + I+ +V A+ E T D++ +KT E+GD + +F
Sbjct: 299 IAQILSLSMLIRYSMNLNEIADKIDLSVHYALKEGYRTLDISDGKNYIKTNEMGDIIAEF 358
Query: 345 LE 346
L+
Sbjct: 359 LD 360
>dbj|BAB08299.1| (AB007650) 3-isopropylmalate dehydrogenase [Arabidopsis thaliana]
Length = 409
Score = 213 bits (538), Expect = 2e-54
Identities = 141/360 (39%), Positives = 202/360 (55%), Gaps = 18/360 (5%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
+ IA++PGDGIG EV++ VL+K L + F+FKE P G G LP+
Sbjct: 46 RYNIALLPGDGIGPEVISVAKNVLQKAGSLEGLEFDFKEMPVGGAALDLVGVPLPEETFT 105
Query: 62 EFKKFDAIYFGAIGD---PRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGK 118
K DAI GAIG + + + L +R +L ++ NLRP + P+L
Sbjct: 106 AAKLSDAILLGAIGGYKWDKNEKHLRPEMALFYLRRDLKVFANLRPATVL-PQLVDASTL 164
Query: 119 NK-----IDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA 172
K +D++ VRE T G+Y G G +E+ V I ++R+ R AFE A
Sbjct: 165 KKEVAEGVDMMIVRELTGGIYFGEPRGITINENGEEVGVSTEIYAAHEIDRIARVAFETA 224
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
++ R K+ VDKANVL A LW + ++ EY D+E H YVD AM++IR P+ FD
Sbjct: 225 RKR-RGKLCSVDKANVLD-ASILWRKRVTALASEYPDVELSHMYVDNAAMQLIRDPKQFD 282
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
+VT N+FGDIL+D + I G +G+ S ++ G+FEP+HGSAPDIAG+ ANPLA
Sbjct: 283 TIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLAT 342
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM----NGNLKTYEVGDWVVKFLE 346
ILSA+M+L+Y L EK +K IE+AV A+ + T D+ N + E+G+ V+K +E
Sbjct: 343 ILSAAMLLKYGLGEEKAAKRIEDAVVDALNKGFRTGDIYSPGNKLVGCKEMGEEVLKSVE 402
>pir||B75160 3-isopropylmalate dehydrogenase (leub) PAB0289 - Pyrococcus abyssi
(strain Orsay) >gi|5457871|emb|CAB49361.1| (AJ248284)
3-isopropylmalate dehydrogenase (leuB) [Pyrococcus
abyssi]
Length = 346
Score = 213 bits (537), Expect = 2e-54
Identities = 136/340 (40%), Positives = 191/340 (56%), Gaps = 27/340 (7%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
R+AVI GDGIG EVV ++V+ + + R F E+ G E + + G + + ++E
Sbjct: 3 RVAVIKGDGIGPEVVDSAIRVVNSVTDRIR----FYEFEGGFEVFKRIGSPISEDDLKEI 58
Query: 64 KKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+K DAI FGA P PG R +++ +R ELDLY NLR + P L+ N +I
Sbjct: 59 RKMDAILFGATTTPFNVPGY--RSLIVTLRKELDLYANLRII----PDLS-----NGKEI 107
Query: 124 VFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
V VRENTEGLYA G GF + A+ I T G R+ +FA AK +T
Sbjct: 108 VIVRENTEGLYARDGIGF------SDRAIDFRIITLEGARRIAKFAINLAKERN-SFITF 160
Query: 183 VDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
V KANVL + + E+++ +E +D+ +K++++P V++T NMFGDI
Sbjct: 161 VHKANVLK-GDRFFREIVLEIAEREGVEVREAIIDSFMIKLVKNPWDHGVILTENMFGDI 219
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
++DL G +GI SGN E +FEP+HGSAPDIAGK IANP+ AILSA+MML+YL
Sbjct: 220 ISDLATIHAGSIGIVPSGNYGDEIA-LFEPIHGSAPDIAGKGIANPIGAILSAAMMLDYL 278
Query: 303 NLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVV 342
L + W EA +R + +TPDM G T EV ++
Sbjct: 279 GLNGKVVW--EATRRYVRYGNLTPDMGGTATTSEVTKGII 316
>sp|P54354|LEU3_BACFR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2117496|pir||I40235 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Bacteroides fragilis
>gi|984575|dbj|BAA08117.1| (D45169) beta-isopropylmalate
dehydrogenase [Bacteroides fragilis]
Length = 353
Score = 213 bits (536), Expect = 3e-54
Identities = 138/362 (38%), Positives = 209/362 (57%), Gaps = 29/362 (8%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M +IAV+ GDGIG E+ +G++V+ + E ++ GA+ K G+ P+
Sbjct: 1 MDFKIAVLAGDGIGPEISVQGVEVMSAVCEKFGHKVNYEYAICGADAIDKVGDPFPEETY 60
Query: 61 EEFKKFDAIYFGAIGDPR--------VKPGILERGILLKMRFELDLYVNLRPVKLYHPRL 112
K DA+ F A+GDP+ V+P E+G LL MR +L L+ N+RPV+ + +
Sbjct: 61 RVCKNADAVLFSAVGDPKFDNDPTAKVRP---EQG-LLAMRKKLGLFANIRPVQTFKCLV 116
Query: 113 --TPLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA 168
+PL+ + D + +RE T G+Y G K + A + TR +ER+++
Sbjct: 117 HKSPLRAELVEGADFLCIRELTGGMYFG-----EKYQDNDKAYDTNMYTRPEIERILKVG 171
Query: 169 FEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSP 227
FEYA + RK +T+VDKANVL + LW ++ E++ +Y ++ TD+ +VD AMKMI+ P
Sbjct: 172 FEYAMKR-RKHLTVVDKANVLASSR-LWRQIAQEMAPQYPEVTTDYMFVDNAAMKMIQEP 229
Query: 228 ESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIAN 287
+ FDV+VT N FGDILTD G+ I G +G+ S + E+T +FEP+HGS P G IAN
Sbjct: 230 KFFDVMVTENTFGDILTDEGSVISGSMGLLPSASTG-ESTPVFEPIHGSWPQAKGLNIAN 288
Query: 288 PLAAILSASMMLEYLNLEKESKWIEEAVKRAIAENKVTPD--MNGNLK--TYEVGDWVVK 343
PLA ILS +M+ EY + + E I +AV ++ N TP+ + G K T EVG W+V
Sbjct: 289 PLAQILSVAMLFEYFDCKAEGALIRKAVDASLDANVRTPEIQVEGGEKFGTKEVGAWIVD 348
Query: 344 FL 345
++
Sbjct: 349 YI 350
>emb|CAA72702.1| (Y11966) 3-isopropylmalate dehydrogenase [Buchnera aphidicola]
Length = 364
Score = 212 bits (534), Expect = 5e-54
Identities = 134/360 (37%), Positives = 208/360 (57%), Gaps = 24/360 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
+IAV+PGDGIG E++ E K++ L++ + + EY G K G L IE
Sbjct: 7 KIAVLPGDGIGPEIIQEAYKIIDVLQKKFSFNVKMHEYDIGGISIDKHGTALTQETIEGC 66
Query: 64 KKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHPRL--TP 114
+ DAI FG++G + KP E+ LLK+R +L+ N+RP KL L +P
Sbjct: 67 ENSDAILFGSVGGSKWDHLPEIEKP---EKAGLLKLRKHFNLFANIRPCKLSQHLLDISP 123
Query: 115 LKG---KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEY 171
LK K ID++ +RE T G+Y G + A I R ++R+ + AF+
Sbjct: 124 LKNSIIKKGIDLICIRELTGGIYFGKSNKITN-IDDPYAFDTEIYFRSEIKRIAKIAFQI 182
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A+ S +KK+ +DKANVL + LW +++ ++ +++ +H Y+D M++I++P SF
Sbjct: 183 AQ-SRKKKIVSIDKANVLK-SSILWRDTVNKMAYKFPNVKLEHMYIDNATMELIKNPSSF 240
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
DV++ N+FGDI++D A I+G +G+ S ++N G++EP GSAP+IAGK IANP+A
Sbjct: 241 DVILCSNLFGDIISDECAAIIGSIGLLPSASLNKSGFGLYEPAGGSAPNIAGKTIANPIA 300
Query: 291 AILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN----LKTYEVGDWVVKFL 345
ILS SM+L+Y L+L S I+EAV A+ + +T D++ N LKT E+GD + L
Sbjct: 301 QILSLSMLLKYSLHLPFLSSCIDEAVHEALVKKYMTMDISKNPQNFLKTNEIGDKISDLL 360
>gb|AAD21684.1| (AC004793) Strong similarity to gb|X59970 3-isopropylmalate
dehydrogenase (IMDH) from Brassica napus. EST gb|F14478
comes from this gene. [Arabidopsis thaliana]
Length = 406
Score = 212 bits (534), Expect = 5e-54
Identities = 137/339 (40%), Positives = 194/339 (56%), Gaps = 16/339 (4%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
+ I ++PGDGIG EV++ VL+K L + F+F+E PFG G LP+
Sbjct: 43 RYNITLLPGDGIGPEVISVAKNVLQKAGFLQGLEFDFQEMPFGGAALDLVGVPLPEETST 102
Query: 62 EFKKFDAIYFGAIG----DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKG 117
K+ DAI GAIG D K E G LL +R +L+++ NLRP + P+L
Sbjct: 103 AAKQSDAILLGAIGGYKWDKNEKHLRPEMG-LLNIRRDLNVFANLRPATVL-PQLVDAST 160
Query: 118 KNK-----IDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFEY 171
K +D++ VRE T G+Y G G +E+ I ++R+ R AFE
Sbjct: 161 LKKEVAQGVDMMIVRELTGGIYFGEPRGITINENGEEVGFNTEIYAAHEIDRIARVAFET 220
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A++ R K+ VDKANVL A LW + ++ EY D+E H YVD AM+++R P+ F
Sbjct: 221 ARKR-RGKLCSVDKANVLD-ASILWRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQF 278
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
D +VT N+FGDIL+D + I G +G+ S ++ G+FEP+HGSAPDIAG+ ANPLA
Sbjct: 279 DTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLA 338
Query: 291 AILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM 328
ILSA+M+L+Y L EK +K IE+AV A+ + T D+
Sbjct: 339 TILSAAMLLKYGLGEEKAAKMIEDAVVDALNKGFRTGDI 377
>sp|P05644|LEU3_BACCA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|98095|pir||A26447 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Bacillus caldotenax
>gi|39406|emb|CAA28455.1| (X04762) 3-isopropylmalate
dehydrogenase (AA 1-366) [Bacillus caldotenax]
Length = 366
Score = 212 bits (533), Expect = 7e-54
Identities = 133/304 (43%), Positives = 185/304 (60%), Gaps = 12/304 (3%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
RIAV+PGDGIGKEV + ++VLK + F F+ G + G LP+ +
Sbjct: 5 RIAVLPGDGIGKEVTSGAVEVLKAVGIRFGHEFTFEYGLIGGAAIDEAGTPLPEETVRLC 64
Query: 64 KKFDAIYFGAIGDPRVK---PGILERGILLKMRFELDLYVNLRPVKLYHPRLT--PLKGK 118
++ DA+ GA+G P+ P + LL +R +LDLY NLRPV Y ++ PLK
Sbjct: 65 RESDAVLLGAVGGPKWDDNPPHLRPEKGLLAIRKQLDLYANLRPVVCYDSLVSRSPLKPD 124
Query: 119 --NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSG 176
+D V VRE T G+Y G + + +E AV ++ + +ER++R AFE A R
Sbjct: 125 LVQGVDFVIVRELTGGIYFGQPSAVVENG-EEKAVDTLLYKKEEIERIVRMAFELA-RGR 182
Query: 177 RKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVT 235
RKKVT VDKANVL+ + LW V EV+ E+ D+ +H VD M M++IR+P+ FDV+VT
Sbjct: 183 RKKVTSVDKANVLSSSR-LWREVAEEVANEFPDVTLEHMLVD-MRMQLIRAPKQFDVIVT 240
Query: 236 PNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSA 295
NMFGDIL+D + + G LG+ S +++ ++EPVHGSAPDIAG ANP+AAILSA
Sbjct: 241 ENMFGDILSDEASMLSGSLGMLPSASLSASGPSLYEPVHGSAPDIAGMNKANPIAAILSA 300
Query: 296 SMML 299
+MML
Sbjct: 301 AMML 304
>pir||E82564 3-isopropylmalate dehydrogenase XF2372 [imported] - Xylella
fastidiosa (strain 9a5c)
>gi|9107550|gb|AAF85171.1|AE004047_2 (AE004047)
3-isopropylmalate dehydrogenase [Xylella fastidiosa]
Length = 357
Score = 212 bits (533), Expect = 7e-54
Identities = 139/361 (38%), Positives = 199/361 (54%), Gaps = 20/361 (5%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M +I ++PGDGIG E+VAE +KVL+ + ++ G Y K G L D +
Sbjct: 1 MSKQILILPGDGIGHEIVAEAVKVLEHVNVRHTLNLRLSFDALGGAAYEKYGSPLADETL 60
Query: 61 EEFKKFDAIYFGAIGDPR---VKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKG 117
+ DA+ GA+G P+ + P + LLK+R +L L+ NLRP LY P+L
Sbjct: 61 ARARDADAVLLGAVGGPQWDTIDPALRPERGLLKIRAQLGLFANLRPALLY-PQLADAST 119
Query: 118 KNK-----IDIVFVRENTEGLYAGA--GGFLRKGTPQEIAVQEMINTRFGVERVIRFAFE 170
+DI+ VRE T GLY G G + K +E A + + R+++ FE
Sbjct: 120 LKPEVVAGLDILIVRELTGGLYFGQPRGSRISKNGERE-AFDTLPYCESEIRRILKVGFE 178
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPES 229
A+ G KK+ VDKANVL + LW V E++++Y D+ H YVD AM+++R P+
Sbjct: 179 MARLRG-KKLCSVDKANVLA-SSQLWRTVAGEMAKDYPDVTLSHMYVDNAAMQLVRHPKQ 236
Query: 230 FDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPL 289
FDV+VT NMFGDIL+D + + G +G+ S +++ GM+EP HGSAPDIAG+ +ANPL
Sbjct: 237 FDVIVTENMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPL 296
Query: 290 AAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM--NGNLK--TYEVGDWVVKF 344
A ILS +M+L Y + K + IE AV + T D+ G K T +GD VV
Sbjct: 297 ATILSVAMLLRYSMGHIKTADAIEHAVGVVLDSGLRTADIWSEGMTKVGTVAMGDAVVAA 356
Query: 345 L 345
L
Sbjct: 357 L 357
>gi|10957108 3-isopropylmalate dehydrogenase [Buchnera sp. APS]
>gi|7707822|dbj|BAA95424.1| (AP001071) 3-isopropylmalate
dehydrogenase [Buchnera sp. APS]
Length = 363
Score = 210 bits (530), Expect = 2e-53
Identities = 139/360 (38%), Positives = 204/360 (56%), Gaps = 24/360 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
RIAV+ GDGIG EV+ E K+L L++ +S E +++ G + G LP +
Sbjct: 6 RIAVLSGDGIGPEVMQEACKILNVLKKYFFLSLEIQKFNIGGIAIEREGVALPKTTLLGC 65
Query: 64 KKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHPR--LTP 114
+ D+I G++G + +P ER LL +R +L+ NLRP KLY L+P
Sbjct: 66 ENSDSILLGSVGGKKWDNLPVEQRP---ERAALLPLRKHFNLFSNLRPAKLYPELKCLSP 122
Query: 115 LKG---KNKIDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFE 170
L+ KN DI+ VRE T G+Y G GF+ K + A I + + R+ AF+
Sbjct: 123 LRSDIVKNGFDILCVRELTGGIYFGEPKGFVNKNNTK-YAFDTEIYHEYEIIRIAHLAFK 181
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPES 229
A RS +KKV +DK+NVL + LW V VS++Y D+ H Y+D AM++I+ P
Sbjct: 182 LA-RSRKKKVCSIDKSNVLQ-SSILWREVVESVSKKYPDVHLSHLYIDNAAMQIIKDPNQ 239
Query: 230 FDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPL 289
FDV++ N+FGDI++D A I G +G+ S + N + G++EP GSAPDI GK IANP+
Sbjct: 240 FDVLLCSNLFGDIISDECATITGSIGMLPSASFNEKNFGLYEPAGGSAPDIEGKNIANPI 299
Query: 290 AAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGNLKTYEVGDWVVKFL 345
A ILS SM++ Y +NL + + I++AV + + T D+ N LKT E+GD +V L
Sbjct: 300 AQILSLSMLVRYGMNLNQIADKIDKAVNNVLKKGYRTSDISHDNNFLKTDEMGDLIVDSL 359
>gb|AAG31405.1| (AF197457) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 210 bits (530), Expect = 2e-53
Identities = 139/360 (38%), Positives = 204/360 (56%), Gaps = 24/360 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
RIAV+ GDGIG EV+ E K+L L++ +S E +++ G + G LP +
Sbjct: 6 RIAVLSGDGIGPEVMQEACKILNVLKKYFFLSLEIQKFNIGGIAIEREGVALPKTTLLGC 65
Query: 64 KKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHPR--LTP 114
+ D+I G++G + +P ER LL +R +L+ NLRP KLY L+P
Sbjct: 66 ENSDSILLGSVGGKKWDNLPVEQRP---ERAALLPLRKHFNLFSNLRPAKLYPELKCLSP 122
Query: 115 LKG---KNKIDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFE 170
L+ KN DI+ VRE T G+Y G GF+ K + A I + + R+ AF+
Sbjct: 123 LRSDIVKNGFDILCVRELTGGIYFGEPKGFVNKNNTK-YAFDTEIYHEYEIIRIAHLAFK 181
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPES 229
A RS +KKV +DK+NVL + LW V VS++Y D+ H Y+D AM++I+ P
Sbjct: 182 LA-RSRKKKVCSIDKSNVLQ-SSILWREVVESVSKKYPDVHLSHLYIDNAAMQIIKDPNQ 239
Query: 230 FDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPL 289
FDV++ N+FGDI++D A I G +G+ S + N + G++EP GSAPDI GK IANP+
Sbjct: 240 FDVLLCSNLFGDIISDECATITGSIGMLPSASFNEKNFGLYEPAGGSAPDIEGKNIANPI 299
Query: 290 AAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGNLKTYEVGDWVVKFL 345
A ILS SM++ Y +NL + + I++AV + + T D+ N LKT E+GD +V L
Sbjct: 300 AQILSLSMLVRYGMNLNQIADKIDKAVNNVLKKGYRTSDISHDNNFLKTDEMGDLIVDSL 359
>sp|P56933|LEU3_BUCAI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|5912537|emb|CAB56191.1| (AJ006878)
3-isopropylmalate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 210 bits (528), Expect = 3e-53
Identities = 139/360 (38%), Positives = 205/360 (56%), Gaps = 24/360 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
RIAV+ GDGIG EV+ E K+L L++ +S E +++ G + G LP +
Sbjct: 6 RIAVLSGDGIGPEVMQEACKILNVLKKYFFLSLEIQKFNIGGIAIEREGVALPKTTLLGC 65
Query: 64 KKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHPR--LTP 114
+ D+I G++G + +P ER LL +R +L+ NLRP KLY L+P
Sbjct: 66 ENSDSILLGSVGGKKWDNLPVEQRP---ERAALLPLRKYFNLFSNLRPAKLYPELKCLSP 122
Query: 115 LKG---KNKIDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFE 170
L+ KN DI+ VRE T G+Y G GF+ K + A I + + R+ AF+
Sbjct: 123 LRSDIVKNGFDILCVRELTGGIYFGEPKGFVNKNNTK-YAFDTEIYHEYEIIRIAHLAFK 181
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPES 229
A RS +KKV +DK+NVL + LW +V VS++Y D+ H Y+D AM++I+ P
Sbjct: 182 LA-RSKKKKVCSIDKSNVLR-SSILWGKVVESVSKKYPDVHLSHLYIDNAAMQIIKDPNQ 239
Query: 230 FDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPL 289
FDV++ N+FGDI++D A I G +G+ S + N + G++EP GSAPDI GK IANP+
Sbjct: 240 FDVLLCSNLFGDIISDECATITGSIGMLPSASFNEKNFGLYEPAGGSAPDIEGKNIANPI 299
Query: 290 AAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGNLKTYEVGDWVVKFL 345
A ILS SM++ Y +NL + + I++AV + + T D+ N LKT E+GD +V L
Sbjct: 300 AQILSLSMLVRYGMNLNQIADKIDKAVNNVLKKGYRTSDISHDNNFLKTDEMGDLIVDSL 359
>emb|CAA71268.1| (Y10216) hypothetical 3-isopropylmalate dehydrogenase [Arabidopsis
thaliana] >gi|6730747|gb|AAF27137.1|AC018849_25
(AC018849) putative 3-isopropylmalate dehydrogenase;
30797-28504 [Arabidopsis thaliana]
Length = 405
Score = 209 bits (527), Expect = 3e-53
Identities = 135/336 (40%), Positives = 192/336 (56%), Gaps = 16/336 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
I ++PGDGIG EVV+ VL++ L V F F+E P G G LP+ I K
Sbjct: 45 ITLLPGDGIGPEVVSIAKNVLQQAGSLEGVEFNFREMPIGGAALDLVGVPLPEETISAAK 104
Query: 65 KFDAIYFGAIG----DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
+ DA+ GAIG D K E+G LL++R L ++ NLRP + P+L +
Sbjct: 105 ESDAVLLGAIGGYKWDNNEKHLRPEKG-LLQIRAALKVFANLRPATVL-PQLVDASTLKR 162
Query: 121 -----IDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKR 174
+D++ VRE T G+Y G G +E+ + ++R+ R AFE A++
Sbjct: 163 EVAEGVDLMVVRELTGGIYFGEPRGIKTNENGEEVGFNTEVYAAHEIDRIARVAFETARK 222
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVV 233
R K+ VDKANVL A LW + ++ EY D+E H YVD AM+++R P+ FD +
Sbjct: 223 R-RGKLCSVDKANVLE-ASILWRKRVTALASEYPDVELSHMYVDNAAMQLVRDPKQFDTI 280
Query: 234 VTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAIL 293
VT N+FGDIL+D + I G +G+ S +++ G+FEP+HGSAPDIAG+ ANPLA IL
Sbjct: 281 VTNNIFGDILSDEASMITGSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKANPLATIL 340
Query: 294 SASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM 328
SA+M+L+Y L EK +K IE+AV A+ T D+
Sbjct: 341 SAAMLLKYGLGEEKAAKRIEDAVLVALNNGFRTGDI 376
>gb|AAG31399.1| (AF197455) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 209 bits (527), Expect = 3e-53
Identities = 133/361 (36%), Positives = 202/361 (55%), Gaps = 22/361 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RIAV+PGDGIG EV+ E K+L L++ + E KE+ G + G LP +
Sbjct: 4 KYRIAVLPGDGIGPEVMREAYKILNILKDDFSLPLEIKEFNIGGAAIDQEGVALPKKTLL 63
Query: 62 EFKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHP--RL 112
+ DAI FG++G + +P ER LL +R +L+ NLRP +LY L
Sbjct: 64 GCENSDAILFGSVGGKKWDNLPINQRP---ERASLLPLRKHFNLFANLRPAQLYSELKNL 120
Query: 113 TPLKG---KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAF 169
+PL+ KN +I+ +RE T G+Y G K E A I + + R+ AF
Sbjct: 121 SPLRSNIIKNGFNILCIRELTGGIYFGKPTGRLKKNNIEYAFDTEIYYNYEINRIAHLAF 180
Query: 170 EYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPE 228
+ ++ + KV +DK+NVL + LW + +VS+ Y D+ H Y+D M++I+ P
Sbjct: 181 QLSQ-TRNYKVCSIDKSNVLN-SSILWREIVKKVSKHYPDVHLSHLYIDNATMQIIKDPN 238
Query: 229 SFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANP 288
FDV++ N+FGDI++D A I G +G+ S ++N + G++EP GSAPDI GK IANP
Sbjct: 239 QFDVLLCSNLFGDIISDECAMITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNIANP 298
Query: 289 LAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVKF 344
+A ILS SM++ Y +NL+K + I+++V + + T D++ N LKT E+GD +
Sbjct: 299 IAQILSLSMLVRYSMNLKKIADKIDQSVISVLKKGYRTADISNNNDYLKTNEMGDVIASA 358
Query: 345 L 345
L
Sbjct: 359 L 359
>sp|P29102|LEU3_BRANA 3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR (BETA-IPM DEHYDROGENASE)
(IMDH) (3-IPM-DH) >gi|81676|pir||S20510
3-isopropylmalate dehydrogenase (EC 1.1.1.85) precursor
- rape >gi|17827|emb|CAA42596.1| (X59970)
3-isopropylmalate dehydrogenase [Brassica napus]
Length = 406
Score = 208 bits (525), Expect = 6e-53
Identities = 133/342 (38%), Positives = 195/342 (56%), Gaps = 22/342 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
+ I ++PGDGIG EV++ VL++ L + F F+E P G G LP+ +
Sbjct: 43 RYNITLLPGDGIGPEVISIAKNVLQQAGSLEGLEFSFQEMPVGGAALDLVGVPLPEETVS 102
Query: 62 EFKKFDAIYFGAIG-------DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTP 114
K+ DA+ GAIG + +KP E G LL++R L ++ NLRP + P+L
Sbjct: 103 AAKESDAVLLGAIGGYKWDKNEKHLKP---ETG-LLQLRAGLKVFANLRPATVL-PQLVD 157
Query: 115 LKGKNK-----IDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFA 168
+ +D++ VRE T G+Y G G +E+ + ++R+ R A
Sbjct: 158 ASTLKREVAEGVDLMVVRELTGGIYFGVPRGIKTNENGEEVGYNTEVYAAHEIDRIARVA 217
Query: 169 FEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSP 227
FE A++ R K+ VDKANVL A LW R ++ EY D+E H YVD AM+++R P
Sbjct: 218 FETARKR-RGKLCSVDKANVLD-ASILWRRRVTALAAEYPDVELSHMYVDNAAMQLVRDP 275
Query: 228 ESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIAN 287
+ FD +VT N+FGDIL+D + I G +G+ S +++ G+FEP+HGSAPDIAG+ AN
Sbjct: 276 KQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLSDSGPGLFEPIHGSAPDIAGQDKAN 335
Query: 288 PLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM 328
PLA ILSA+M+L+Y L EK +K IE+AV A+ + T D+
Sbjct: 336 PLATILSAAMLLKYGLGEEKAAKRIEDAVLGALNKGFRTGDI 377
>gb|AAG31393.1| (AF197453) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 208 bits (525), Expect = 6e-53
Identities = 134/358 (37%), Positives = 199/358 (55%), Gaps = 16/358 (4%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RIA++PGDGIG EV+ E K+L L+ + EF+E+ G G LP +
Sbjct: 4 KYRIAILPGDGIGPEVMREAYKILNILKNDFLLPLEFQEFNIGGIAIDNEGLALPKKTLL 63
Query: 62 EFKKFDAIYFGAIGDPRVKPGIL----ERGILLKMRFELDLYVNLRPVKLYHPR--LTPL 115
+ DAI FG++G + + ER LL +R +L+ NLRP KLY L+PL
Sbjct: 64 GCENADAILFGSVGGKKWDTLPIDQRPERAALLPLRKHFNLFANLRPAKLYSELEYLSPL 123
Query: 116 KGK---NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA 172
K N DI+ VRE T G+Y G K E A + + + R+ AF+ A
Sbjct: 124 CSKITKNGFDILCVRELTGGVYFGHPHGRVKKDDIEYAFDTEVYYDYEINRIAHLAFQLA 183
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
R+ + KV +DK+NVL + LW+ + +VS Y D+ H Y+D M++I++P FD
Sbjct: 184 -RNRKNKVCSIDKSNVLNSSL-LWKEIVEKVSNSYSDVRLSHLYIDNATMQIIKNPNQFD 241
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
V++ N+FGDI++D A I G +G+ S ++N G++EP GSAPDI GK IANP+A
Sbjct: 242 VLLCSNLFGDIISDECAAISGSIGMLPSASLNENNFGLYEPAGGSAPDIEGKNIANPIAQ 301
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVKFL 345
ILS SM++ Y ++L K + I+++V + T D++ N LKT+E+GD + L
Sbjct: 302 ILSVSMLVRYGMHLTKIADQIDKSVISVLKRGYRTADISNNNNYLKTHEMGDVIADAL 359
>gb|AAG31928.1|AF200469_2 (AF200469) leuB [Buchnera aphidicola]
Length = 363
Score = 208 bits (525), Expect = 6e-53
Identities = 132/358 (36%), Positives = 199/358 (54%), Gaps = 16/358 (4%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RIAV+PGDGIG EV+ E K+L L+ + E +E+ G + G LP +
Sbjct: 4 KYRIAVLPGDGIGPEVMREAYKILNILKNHFSLPLETREFNIGGSAIDQEGTALPKKTLL 63
Query: 62 EFKKFDAIYFGAIGDPRVKPGIL----ERGILLKMRFELDLYVNLRPVKLYHPR--LTPL 115
+ DAI FG++G + + ER LL +R +L+ NLRP KLY L+PL
Sbjct: 64 GCENSDAILFGSVGGKKWDDLPINQRPERASLLPLRKHFNLFANLRPAKLYSELKYLSPL 123
Query: 116 KG---KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA 172
+ KN DI+ +RE T GLY G K E A I + + R+ AF+ A
Sbjct: 124 RSDIIKNGFDILCIRELTGGLYFGKPTGRLKKNNIEYAFDTEIYYNYEITRIAHLAFQLA 183
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
+ + K+ +DK+NVL + LW + +VS+ Y D+ H Y+D M++I+ P FD
Sbjct: 184 Q-TRNFKICSIDKSNVLN-SSVLWREIVKKVSKNYPDVHLSHLYIDNATMQIIKDPNQFD 241
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
+++ N+FGDI++D A I G +G+ S ++N + G++EP GSAPDI GK IANP+A
Sbjct: 242 ILLCSNLFGDIISDECAIITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNIANPIAQ 301
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVKFL 345
ILS SM++ Y +NL+ + I+++V + + T D++ N LKT E+GD + L
Sbjct: 302 ILSVSMLVRYGMNLKTIADKIDQSVISVLKKGYRTADISNNNNYLKTNEMGDVIANTL 359
>pir||E75368 probable isocitrate dehydrogenase - Deinococcus radiodurans
(strain R1) >gi|6459438|gb|AAF11228.1|AE002009_8
(AE002009) isocitrate dehydrogenase, putative
[Deinococcus radiodurans]
Length = 333
Score = 206 bits (519), Expect = 3e-52
Identities = 132/346 (38%), Positives = 201/346 (57%), Gaps = 18/346 (5%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RI +I GDGIG EV+ +VL E + E+ G E++L G ++P+ +
Sbjct: 3 KYRICLIEGDGIGHEVIPAAKRVL----EAAGFDAEYVHAEAGYEYFLDHGTSVPEATYD 58
Query: 62 EFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKI 121
+ DA FGA P + G + +R + +LY N+RP K R P +N +
Sbjct: 59 AVENTDATLFGAATSPSGEKPAGFFGAIRHLRQKYNLYANVRPTKT---RPVPHSYEN-V 114
Query: 122 DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA-KRSGRKKV 180
D+V VRENT+GLY ++ + A+ + + TR +R+ +FA + A KRS K++
Sbjct: 115 DLVIVRENTQGLYVE-----QERRYGDTAIADTVITREASDRIGKFAADLAMKRS--KRL 167
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T+V K+NVL L+ + ++ + L T VD AM+++R+P+ FDV+V NMF
Sbjct: 168 TVVHKSNVLPVTQGLFMNTILDHTKTVEGLSTSTMIVDNAAMQLVRNPQQFDVMVMTNMF 227
Query: 240 GDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
GDIL+DL A +VGGLGIAASGN+ + G+FE VHGSAPDIAG+ I+NP A IL+A +ML
Sbjct: 228 GDILSDLAAGLVGGLGIAASGNVG-DQFGIFESVHGSAPDIAGQGISNPTATILAAVIML 286
Query: 300 EYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
++L + ++ ++ A+ + +AE T D+ G T E + V+K L
Sbjct: 287 DHLGDHETARRLDNAINKVLAEXPRTRDLGGTAGTQEFTEAVIKAL 332
>pir||E81836 probable 3-isopropylmalate dehydrogenase (EC 1.1.1.85) NMA1456
[imported] - Neisseria meningitidis (group A strain
Z2491) >gi|7380107|emb|CAB84691.1| (AL162756) putative
3-isopropylmalate dehydrogenase [Neisseria meningitidis
Z2491]
Length = 356
Score = 206 bits (519), Expect = 3e-52
Identities = 139/362 (38%), Positives = 206/362 (56%), Gaps = 29/362 (8%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKL-EELSRVSFEFKEYPFGAEHYLKTGETLPDWA 59
M IA++ GDGIG E+VAE ++VL KL + V +E+ P G E Y + G P++
Sbjct: 1 MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDVGYEYA--PLGGEAYDEYGHPYPEFT 58
Query: 60 IEEFKKFDAIYFGAIGDPRV----KPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPL 115
+K DA+ GA+G P+ +P ERG LL +R +L+L+ NLRP LY
Sbjct: 59 QNLCRKADAVLLGAVGSPQYDNLDRPLRPERG-LLAIRKDLNLFANLRPAILYPELANAS 117
Query: 116 KGKNKI----DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT----RFGVERVIRF 167
K++I DI+ VRE T +Y G +R E +E NT + R+
Sbjct: 118 TLKSEIVAGLDILIVRELTGDIYFGEPRGIRV---LENGEREGYNTMKYSESEIRRIAHV 174
Query: 168 AFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRS 226
AF+ A++ KKV V KANVL +LW +F E+ +EY D+E H YVD AM+++R+
Sbjct: 175 AFQSAQKRS-KKVCSVGKANVLETT-ELWREIFEEIGKEYPDVELSHMYVDNAAMQLVRA 232
Query: 227 PESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIA 286
P+ FDV+ T N+FGDIL+D + + G +G+ S +++ G++EP HGSAPDIAG+ A
Sbjct: 233 PKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLYEPSHGSAPDIAGQNKA 292
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
NPLA +LS +M+L Y LN E ++ +E AV++ + + T D+ YE G +V
Sbjct: 293 NPLATVLSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGDI------YEEGTKLVSCS 346
Query: 346 EV 347
E+
Sbjct: 347 EM 348
>pir||E81130 3-isopropylmalate dehydrogenase NMB1031 [imported] - Neisseria
meningitidis (group B strain MD58)
>gi|7226270|gb|AAF41430.1| (AE002453) 3-isopropylmalate
dehydrogenase [Neisseria meningitidis MC58]
Length = 356
Score = 205 bits (516), Expect = 7e-52
Identities = 138/361 (38%), Positives = 203/361 (56%), Gaps = 27/361 (7%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M IA++ GDGIG E+VAE ++VL KL + ++ P G E Y + G P++
Sbjct: 1 MTKHIAILRGDGIGPEIVAETVRVLDKLIAQG-LDAGYEYAPLGGEAYDEYGHPYPEFTQ 59
Query: 61 EEFKKFDAIYFGAIGDPRV----KPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLK 116
+K DA+ GA+G P+ +P ERG LL +R +L+L+ NLRP LY
Sbjct: 60 NLCRKADAVLLGAVGSPQYDNLDRPLRPERG-LLAIRKDLNLFANLRPAVLYPELANAST 118
Query: 117 GKNKI----DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT----RFGVERVIRFA 168
K +I DI+ VRE T +Y G +R E +E NT + R+ A
Sbjct: 119 LKPEIVAGLDILIVRELTGDIYFGEPRGIRV---LENGEREGYNTMKYSESEIRRIAHVA 175
Query: 169 FEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSP 227
F+ A++ KKV V KANVL +LW +F E+ +EY D+E H YVD AM+++R+P
Sbjct: 176 FQSAQKRS-KKVCSVGKANVLETT-ELWREIFEEIGKEYPDVELSHMYVDNAAMQLVRAP 233
Query: 228 ESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIAN 287
+ FDV+ T N+FGDIL+D + + G +G+ S +++ G++EP HGSAPDIAG+ AN
Sbjct: 234 KQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLYEPSHGSAPDIAGQNKAN 293
Query: 288 PLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLE 346
PLA ILS +M+L Y LN E ++ +E AV++ + + T D+ YE G +V E
Sbjct: 294 PLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSDI------YEEGTKLVSCSE 347
Query: 347 V 347
+
Sbjct: 348 M 348
>gb|AAG31396.1| (AF197454) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 365
Score = 205 bits (516), Expect = 7e-52
Identities = 134/364 (36%), Positives = 203/364 (54%), Gaps = 26/364 (7%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RI+V+PGDGIG EV+ E K+L L+ + E KE+ G + G LP +
Sbjct: 4 KYRISVLPGDGIGPEVMREAYKILNILKNHFSLPLEIKEFNIGGAAIDQEGVALPKKTLL 63
Query: 62 EFKKFDAIYFGAIGDPR-------VKPGILERGILLKMRFELDLYVNLRPVKLYHPR--L 112
+ DAI FG++G + +P ER LL +R +L+ NLRP +LY L
Sbjct: 64 GCENSDAILFGSVGGKKWDHLPIDKRP---ERASLLPLRKHFNLFANLRPAQLYSELKYL 120
Query: 113 TPLKG----KNKIDIVFVRENTEGLYAG--AGGFLRKGTPQEIAVQEMINTRFGVERVIR 166
+PL+ KN +I+ +RE T G+Y G G L+K E A I + + R+
Sbjct: 121 SPLRSDIXIKNGFNILCIRELTGGIYFGKPTGRXLKKNNI-EYAFDTEIYYDYEINRIAH 179
Query: 167 FAFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIR 225
AF+ A+ + KV +DK+NVL + LW +VS+ Y D+ H Y+D M++I+
Sbjct: 180 LAFQLAQ-TRNYKVCSIDKSNVLN-SSVLWRETVQKVSKNYPDVHLSHLYIDNATMQIIK 237
Query: 226 SPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRI 285
P FD+++ N+FGDI++D A I G +G+ S ++N + G++EP GSAPDI GK I
Sbjct: 238 DPNQFDILLCSNLFGDIVSDECAIITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNI 297
Query: 286 ANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWV 341
ANP+A ILS SM++ Y +NL+K + I+++V + + T D++ N LKT E+GD +
Sbjct: 298 ANPIAQILSVSMLVRYGMNLKKIADKIDQSVISVLKKGYRTADISNNNNYLKTNEMGDVI 357
Query: 342 VKFL 345
L
Sbjct: 358 ANTL 361
>gb|AAK41021.1| 3-isopropylmalate dehydrogenase (leuB) [Sulfolobus solfataricus]
Length = 336
Score = 205 bits (515), Expect = 9e-52
Identities = 133/354 (37%), Positives = 196/354 (54%), Gaps = 27/354 (7%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M R+AVIPGDGIG E+ +++ KL E + + E G K GE LP +
Sbjct: 1 MTFRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTL 60
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN- 119
+ +K D I G +G+ + +++K+R D+Y N+RP K L ++ K
Sbjct: 61 DVIEKADMILKGPVGETAMD-------VVVKLRQMYDMYANIRPAK----SLPNVQSKYP 109
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKK 179
+D+V VRENTE LY G G + + + T F +R++ A YA R+K
Sbjct: 110 NVDLVIVRENTEDLYKGFEFVTSTG----VTIAIKVTTEFASKRIVNVALNYALMR-RRK 164
Query: 180 VTLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
VT V KANV+ L+ V E+ + + + YVDA A ++R P FDV+VT N++
Sbjct: 165 VTCVHKANVMRVTDGLFASVCREILKG-KVNFEEMYVDAAAANLVRDPTRFDVIVTSNVY 223
Query: 240 GDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
GDIL+D ++I G LG+A S NI + MFEPVHG+A DIAGK IANP A +LS SMML
Sbjct: 224 GDILSDEASQIAGSLGLAPSANIG-DRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMML 282
Query: 300 EYL-NLEKESKWI--EEAVKRAIAE-----NKVTPDMNGNLKTYEVGDWVVKFL 345
+ L KE+K+I ++++ AI E NK+T D+ G+ K ++ D + K++
Sbjct: 283 SRMYELSKENKYILASKSLENAIIETYKSGNKLTEDVGGSAKLKDMVDEIYKYM 336
>sp|P73960|LEU3_SYNY3 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7431318|pir||S75467 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Synechocystis sp. (strain
PCC 6803) >gi|1653112|dbj|BAA18028.1| (D90911)
3-isopropylmalate dehydrogenase [Synechocystis sp.]
Length = 362
Score = 205 bits (515), Expect = 9e-52
Identities = 128/335 (38%), Positives = 192/335 (57%), Gaps = 14/335 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDGIG E++A + VL K+ + +F F+E G TG+ LP+ + + K
Sbjct: 7 VTLLPGDGIGPEIMAVAVAVLGKVADQFGFAFNFQEALIGGAAIDATGQPLPEATLAQAK 66
Query: 65 KFDAIYFGAIG----DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
DA+ AIG D + E G LL +R L L+ NLRP ++ P+L +
Sbjct: 67 DSDAVLLAAIGGYAWDNLPRSQRPETG-LLAIREGLGLFANLRPATIF-PQLIDASSLKR 124
Query: 121 -----IDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKR 174
+DI+ VRE T G+Y G G T ++ V M T ++R+ + AFE A++
Sbjct: 125 EVVEGVDIMVVRELTGGIYFGKPKGIFETETGEKRGVNTMAYTVGEIDRIAKVAFETARK 184
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVV 234
R ++ VDKANVL + +RV A D+E H YVD AM+++RSP FD +V
Sbjct: 185 R-RGQLCSVDKANVLDVSQLWRDRVMAIAVDYPDVELSHLYVDNAAMQLVRSPRQFDTIV 243
Query: 235 TPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILS 294
T N+FGDIL+D+ A + G +G+ S ++ + G+FEPVHGSAPDIAG+ ANPLA +LS
Sbjct: 244 TGNLFGDILSDIAAMLTGSIGMLPSASLGSDGPGLFEPVHGSAPDIAGQDKANPLAQVLS 303
Query: 295 ASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM 328
A+MML Y L+ + + +E+AVK+ + + T D+
Sbjct: 304 AAMMLRYGLDQPQAADRLEDAVKKVLEQGYRTGDI 338
>emb|CAB57580.1| (Y18930) 3-isopropylmalate dehydrogenase [Sulfolobus solfataricus]
Length = 336
Score = 205 bits (515), Expect = 9e-52
Identities = 133/354 (37%), Positives = 196/354 (54%), Gaps = 27/354 (7%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M R+AVIPGDGIG E+ +++ KL E + + E G K GE LP +
Sbjct: 1 MTFRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTL 60
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN- 119
+ +K D I G +G+ + +++K+R D+Y N+RP K L ++ K
Sbjct: 61 DVIEKADMILKGPVGETAMD-------VVVKLRQMYDMYANIRPAK----SLPNVQSKYP 109
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKK 179
+D+V VRENTE LY G G + + + T F +R++ A YA R+K
Sbjct: 110 NVDLVIVRENTEDLYKGFEFVTSTG----VTIAIKVTTEFASKRIVNVALNYALMR-RRK 164
Query: 180 VTLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
VT V KANV+ L+ V E+ + + + YVDA A ++R P FDV+VT N++
Sbjct: 165 VTCVHKANVMRVTDGLFASVCREILKG-KVNFEEMYVDAAAANLVRDPTRFDVIVTSNVY 223
Query: 240 GDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
GDIL+D ++I G LG+A S NI + MFEPVHG+A DIAGK IANP A +LS SMML
Sbjct: 224 GDILSDEASQIAGSLGLAPSANIG-DRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMML 282
Query: 300 EYL-NLEKESKWI--EEAVKRAIAE-----NKVTPDMNGNLKTYEVGDWVVKFL 345
+ L KE+K+I ++++ AI E NK+T D+ G+ K ++ D + K++
Sbjct: 283 SRMYELSKENKYILASKSLENAIIETYKSGNKLTEDVGGSAKLKDMVDEIYKYM 336
>gi|10954459 3-isopropylmalate dehydrogenase [Buchnera aphidicola]
>gi|3282741|gb|AAD12601.1| (AF041837) 3-isopropylmalate
dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 204 bits (513), Expect = 2e-51
Identities = 136/356 (38%), Positives = 197/356 (55%), Gaps = 16/356 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
IAV+PGDGIG EV+ E K+L+ L E + + KE+ G G LP + +
Sbjct: 7 IAVLPGDGIGPEVMQEAYKILQVLREHFSLFIKTKEFDIGGIAIDNHGIALPKKTLIGCE 66
Query: 65 KFDAIYFGAIGDPRVKPGIL----ERGILLKMRFELDLYVNLRPVKLYHPR--LTPLKG- 117
DAI G+IG + + ER LL +R + + NLRP LY L+PL+
Sbjct: 67 NSDAILLGSIGGKKWDTLPINERPERASLLPLRKHFNFFCNLRPSNLYKELNFLSPLRND 126
Query: 118 --KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRS 175
K+ DI+ VRE T G+Y G A I +F + R+ AF+ A RS
Sbjct: 127 IVKHGFDILCVRELTGGIYFGKPRGRVTKKKLMYAFDTEIYYKFEIVRIAHLAFKLA-RS 185
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVV 234
+ K+ +DKANVL + LW V EVS+EY D+ H Y+D + M++I+ P FDV++
Sbjct: 186 RKHKLCSIDKANVLE-SSILWREVVEEVSKEYPDVILSHLYIDNVCMQIIKDPNQFDVLL 244
Query: 235 TPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILS 294
N+FGDI++D A I G +G+ S ++N + G++EP GSAPDI GK IANP+A ILS
Sbjct: 245 CSNLFGDIISDECAMITGSIGMLPSASLNEKNFGLYEPAGGSAPDIQGKNIANPIAQILS 304
Query: 295 ASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVKFLE 346
SM++ Y +NL K + I+ AV + + T D++ + LKT E+GD + FL+
Sbjct: 305 LSMLIRYSMNLNKIANKIDNAVINVLKKGYKTMDISKDQNYLKTNEMGDVIADFLK 360
>sp|P50455|LEU3_SULS7 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1890070|dbj|BAA13178.1| (D86857)
3-isopropylmalate dehydrogenase [Sulfolobus sp. 7]
Length = 337
Score = 202 bits (508), Expect = 6e-51
Identities = 132/342 (38%), Positives = 186/342 (53%), Gaps = 25/342 (7%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M +A+I GDGIG E+V++ ++L K+ EL + E+ E G + GE LP ++
Sbjct: 1 MGFTVALIQGDGIGPEIVSKSKRILAKINELYSLPIEYIEVEAGDRALARYGEALPKDSL 60
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
+ K D I G +G+ +++K+R D+Y N+RP K P + G
Sbjct: 61 KIIDKADIILKGPVGESAAD-------VVVKLRQIYDMYANIRPAKSI-PGIDTKYGN-- 110
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+DI+ VRENTE LY G + G +AV I TRF ER+ + +A R RKKV
Sbjct: 111 VDILIVRENTEDLYKGFEHIVSDG----VAVGMKIITRFASERIAKVGLNFALRR-RKKV 165
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDVVVTPNMFG 240
T V KANV+ L+ V + +E YVDA A ++R+P+ FDV+VT N++G
Sbjct: 166 TCVHKANVMRITDGLFAEACRSVLKG-KVEYSEMYVDAAAANLVRNPQMFDVIVTENVYG 224
Query: 241 DILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLE 300
DIL+D ++I G LGIA S NI + +FEPVHG+A DIAGK I NP A +LS SMM E
Sbjct: 225 DILSDEASQIAGSLGIAPSANIG-DKKALFEPVHGAAFDIAGKNIGNPTAFLLSVSMMYE 283
Query: 301 YL-------NLEKESKWIEEAVKRAIAENK-VTPDMNGNLKT 334
+ K S+ +E A+ E K +TPD+ GN T
Sbjct: 284 RMYELSNDDRYIKASRALENAIYLVYKERKALTPDVGGNATT 325
>pir||A82539 isocitrate dehydrogenase XF2596 [imported] - Xylella fastidiosa
(strain 9a5c) >gi|9107816|gb|AAF85393.1|AE004066_7
(AE004066) isocitrate dehydrogenase [Xylella fastidiosa]
Length = 335
Score = 202 bits (508), Expect = 6e-51
Identities = 125/338 (36%), Positives = 190/338 (55%), Gaps = 14/338 (4%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M I VI GDGIG E++ L VL L+ + +++EF + A K G+ LP+ +
Sbjct: 1 MTQTITVIRGDGIGPEIMDATLLVLNTLQ--ANLNYEFADAGLNALE--KHGDLLPEATL 56
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
K + P V G + L+ +F DLY N+RP K + T + +
Sbjct: 57 NSISKNKVALKSPLTTP-VGEGFSSINVALRRKF--DLYANVRPAKSFPN--TKSRFSDN 111
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+D++ VRENTEG Y G + E+A TR G ER++R+AF+ A+ +GRKKV
Sbjct: 112 VDLITVRENTEGAYLSEGQTI--SPDGEVATSSARVTRKGSERIVRYAFDLARTTGRKKV 169
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T V KAN++ L+ + +V+ +Y +++ VD M+++ PE FD++VT N+F
Sbjct: 170 TAVHKANIIKSTSGLFLKTARDVASQYPEIDFQEMIVDNACMQLVMRPEQFDIIVTTNLF 229
Query: 240 GDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
GDIL+DL A +VGGLG+A NI E +FE VHGSAPDIAG+ ANP A +L A ML
Sbjct: 230 GDILSDLCAGLVGGLGLAPGANIGNE-QAIFEAVHGSAPDIAGQGKANPCALLLGAVQML 288
Query: 300 EYLNLEKESKWIEEAVKRAI-AENKVTPDMNGNLKTYE 336
+++ + ++ + A+ + A++ +TPD+ GN T E
Sbjct: 289 DHIGQPQNAERLRNAIVATLNAKDSLTPDLGGNGNTME 326
>sp|P41019|LEU3_BACME 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2117493|pir||I40226 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Bacillus megaterium
>gi|414097|emb|CAA46295.1| (X65184)
3-isopropylmalat-dehydrogenase [Bacillus megaterium]
>gi|1095455|prf||2109196A 3-isopropylmalate
dehydrogenase [Bacillus megaterium]
Length = 367
Score = 201 bits (506), Expect = 1e-50
Identities = 144/362 (39%), Positives = 200/362 (54%), Gaps = 17/362 (4%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M ++AV+PGDG G+ + LK + E R FEF G + G + I
Sbjct: 1 MTKKVAVLPGDG-GQSNQRRCCR-LKAIGERLRHQFEFTYGLIGGAAIDEAGSSSSRSTI 58
Query: 61 EEFKKFDAIYFGAIGDPRVK--PGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL--TP 114
E + DA+ G++G P+ P L E+G LL +R ELDLY NLRPV Y +P
Sbjct: 59 ETCNEADAVLLGSVGGPKWDRFPSHLRPEKG-LLAIRKELDLYANLRPVTFYDSLADASP 117
Query: 115 LKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA 172
LK + +D + VRE T GLY G R +E V + R +ER+I
Sbjct: 118 LKKEYIEGVDFIIVRELTGGLYFGKPSDRRTEGNKETVVDTLFYKRTEIERIIASIDTAV 177
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
R RKKVT VDKANVL + +W V EV+++Y D+E +H VD+ AM++IR+P+ FD
Sbjct: 178 NR--RKKVTSVDKANVLESSR-VWREVAEEVAKDYPDVELEHMLVDSAAMQLIRNPKYFD 234
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPL-A 290
VVVT NMFGDIL+D + + G LG+ S ++ + ++EPVHGSAPDIAG+ ANP+ A
Sbjct: 235 VVVTENMFGDILSDEASMLTGSLGMLPSASLTADGPSLYEPVHGSAPDIAGQNKANPIAA 294
Query: 291 AILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLEVVA 349
AILSA+M+L + LEKE+ IE+AV+ + T D+ V V+A
Sbjct: 295 AILSAAMLLRHSFGLEKEAAVIEQAVESVLHAGHRTADLADGNHYLGTDKMVEVITAVIA 354
Query: 350 NE 351
N+
Sbjct: 355 ND 356
>gi|6322097 Homo-isocitrate dehydrogenase; Lys12p [Saccharomyces cerevisiae]
>gi|731845|sp|P40495|YIJ4_YEAST HYPOTHETICAL 40.1 KD
PROTEIN IN SGA1-KTR7 INTERGENIC REGION
>gi|1076985|pir||S49786 3-isopropylmalate dehydrogenase
homolog YIL094c - yeast (Saccharomyces cerevisiae)
>gi|577120|emb|CAA86700.1| (Z46728) YI9910.02c, orf,
len: 371, CAI: 0.31, similar to isocitrate and
isopropylmalate dehyrogenases [Saccharomyces cerevisiae]
Length = 371
Score = 201 bits (505), Expect = 1e-50
Identities = 133/358 (37%), Positives = 194/358 (54%), Gaps = 25/358 (6%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
L I +IPGDGIGKEV+ G +VL+ L +SF F + G + + +TG+ LPD ++
Sbjct: 24 LTIGLIPGDGIGKEVIPAGKQVLENLNSKHGLSFNFIDLYAGFQTFQETGKALPDETVKV 83
Query: 63 FKK-FDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKI 121
K+ FGA+ P K I+ +R E+ L+ N+RPVK KGK I
Sbjct: 84 LKEQCQGALFGAVQSPTTKVEGYSSPIVA-LRREMGLFANVRPVKSVEGE----KGK-PI 137
Query: 122 DIVFVRENTEGLYAGAGG-FLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRS----G 176
D+V VRENTE LY ++ K T +A + R+ A + A + G
Sbjct: 138 DMVIVRENTEDLYIKIEKTYIDKATGTRVADATKRISEIATRRIATIALDIALKRLQTRG 197
Query: 177 RKKVTLVDKANVLTYAHDLWERVFAEVSQEYDLETDHY--------YVDAMAMKMIRSPE 228
+ +T+ K+NVL+ + L+ + EV Y+ D Y VD+M ++ R P+
Sbjct: 198 QATLTVTHKSNVLSQSDGLFREICKEV---YESNKDKYGQIKYNEQIVDSMVYRLFREPQ 254
Query: 229 SFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANP 288
FDV+V PN++GDIL+D A +VG LG+ S N+ PE + EP HGSAPDIAGK IANP
Sbjct: 255 CFDVIVAPNLYGDILSDGAAALVGSLGVVPSANVGPEIV-IGEPCHGSAPDIAGKGIANP 313
Query: 289 LAAILSASMMLEYLNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFL 345
+A I S ++MLE+L + ++ I +AV + E + TPD+ G T +V D V+ L
Sbjct: 314 IATIRSTALMLEFLGHNEAAQDIYKAVDANLREGSIKTPDLGGKASTQQVVDDVLSRL 371
>sp|P33197|IDH_THETH ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCINATE DECARBOXYLASE)
(IDH) (NADP+-SPECIFIC ICDH) (IDP)
>gi|155102|gb|AAA27492.1| (M94317) isocitrate
dehydrogenase [Thermus thermophilus]
Length = 495
Score = 199 bits (502), Expect = 3e-50
Identities = 133/346 (38%), Positives = 194/346 (55%), Gaps = 19/346 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLK-TGETLPDWAIEEF 63
I VIPGDGIG E V LKVL+ + + +++E +E GA + + +P IE
Sbjct: 22 ITVIPGDGIGPECVEATLKVLEAAK--APLAYEVREA--GASVFRRGIASGVPQETIESI 77
Query: 64 KKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+K + G + P G E+ + +R + Y N+RPV+ + TP G+ ID+
Sbjct: 78 RKTRVVLKGPLETP---VGYGEKSANVTLRKLFETYANVRPVREFPNVPTPYAGRG-IDL 133
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
V VREN E LYAG TP ++I+ + G E+++RFAFE A+ GRKKV
Sbjct: 134 VVVRENVEDLYAGIE---HMQTPSVAQTLKLISWK-GSEKIVRFAFELARAEGRKKVHCA 189
Query: 184 DKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
K+N++ A +R F +V+QEY D+E H VD A ++++ PE F+V+VT NM GDI
Sbjct: 190 TKSNIMKLAEGP-KRAFEQVAQEYPDIEAVHIIVDNAAHQLVKRPEQFEVIVTTNMNGDI 248
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
L+DL + ++GGLG A S NI E +FE VHGSAP AGK + NP A +LSA MML YL
Sbjct: 249 LSDLTSGLIGGLGFAPSANIGNE-VAIFEAVHGSAPKYAGKNVINPTAVLLSAVMMLRYL 307
Query: 303 NLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLEVV 348
+ IE A+ + E +V + G++ Y+ G ++ E +
Sbjct: 308 EEFATADLIENALLYTLEEGRV---LTGDVVGYDRGAKTTEYTEAI 350
>pir||A43934 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) - Thermus aquaticus
Length = 494
Score = 199 bits (502), Expect = 3e-50
Identities = 133/346 (38%), Positives = 194/346 (55%), Gaps = 19/346 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLK-TGETLPDWAIEEF 63
I VIPGDGIG E V LKVL+ + + +++E +E GA + + +P IE
Sbjct: 21 ITVIPGDGIGPECVEATLKVLEAAK--APLAYEVREA--GASVFRRGIASGVPQETIESI 76
Query: 64 KKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+K + G + P G E+ + +R + Y N+RPV+ + TP G+ ID+
Sbjct: 77 RKTRVVLKGPLETP---VGYGEKSANVTLRKLFETYANVRPVREFPNVPTPYAGRG-IDL 132
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
V VREN E LYAG TP ++I+ + G E+++RFAFE A+ GRKKV
Sbjct: 133 VVVRENVEDLYAGIE---HMQTPSVAQTLKLISWK-GSEKIVRFAFELARAEGRKKVHCA 188
Query: 184 DKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
K+N++ A +R F +V+QEY D+E H VD A ++++ PE F+V+VT NM GDI
Sbjct: 189 TKSNIMKLAEGP-KRAFEQVAQEYPDIEAVHIIVDNAAHQLVKRPEQFEVIVTTNMNGDI 247
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
L+DL + ++GGLG A S NI E +FE VHGSAP AGK + NP A +LSA MML YL
Sbjct: 248 LSDLTSGLIGGLGFAPSANIGNE-VAIFEAVHGSAPKYAGKNVINPTAVLLSAVMMLRYL 306
Query: 303 NLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLEVV 348
+ IE A+ + E +V + G++ Y+ G ++ E +
Sbjct: 307 EEFATADLIENALLYTLEEGRV---LTGDVVGYDRGAKTTEYTEAI 349
>gb|AAG31378.1| (AF197448) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 364
Score = 198 bits (498), Expect = 9e-50
Identities = 131/359 (36%), Positives = 195/359 (53%), Gaps = 17/359 (4%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RIAV+PGDGIG EV+ E K+L L+ + E +E+ G K G LP +
Sbjct: 4 KYRIAVLPGDGIGPEVMREAYKILNILKYHFSLPLEIEEFDVGGSAIDKEGVALPKKTLL 63
Query: 62 EFKKFDAIYFGAIGDPRVKPGIL----ERGILLKMRFELDLYVNLRPVKLYHPR--LTPL 115
+ DAI FG++G + + ER LL +R +L+ NLRP +LY L+PL
Sbjct: 64 GCENADAILFGSVGGRKWDSLPIDQRPERASLLPLRKHFNLFANLRPAQLYSELKYLSPL 123
Query: 116 KG----KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEY 171
KN +I+ VRE T G+Y G K E A + + + R+ AF+
Sbjct: 124 AFLIVIKNGFNILCVRELTGGIYFGQPSGRSKTNNFEYAFDTEVYYDYEINRIAHLAFQL 183
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESF 230
A+ + KV +DK+NVL + LW + +VS Y ++ H Y+D M++I+ P F
Sbjct: 184 AQ-TRNYKVCSIDKSNVLN-SSILWREIVKKVSLSYPNVHLSHLYIDNATMQIIKDPNQF 241
Query: 231 DVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLA 290
DV++ N+FGDI++D A I G +G+ S ++N + G++EP GSAPDI GK IANP+A
Sbjct: 242 DVLLCSNLFGDIISDECAIITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNIANPIA 301
Query: 291 AILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM---NGNLKTYEVGDWVVKFL 345
ILS SM++ Y +NL+ + I+ +V + + T D+ N LKT E+GD + L
Sbjct: 302 QILSVSMLVRYSMNLKNIADKIDYSVISILKKGYKTADIAHDNNYLKTNEMGDVIADAL 360
>sp|P50180|LEU3_NEILA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|628597|pir||S43888 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Neisseria lactamica
(fragment) >gi|476229|gb|AAA53239.1| (U06074)
isopropylmalate dehydrogenase [Neisseria lactamica]
Length = 313
Score = 197 bits (496), Expect = 2e-49
Identities = 127/314 (40%), Positives = 181/314 (57%), Gaps = 20/314 (6%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M IA++ GDGIG E+VAE ++VL KL E + ++ P G E Y + G P++
Sbjct: 1 MTKHIAILRGDGIGPEIVAETVRVLDKLIEQG-LDAGYEYAPLGGEAYDEYGHPYPEFTQ 59
Query: 61 EEFKKFDAIYFGAIGDPRV----KPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLK 116
+K DA+ GA+G P+ +P ERG LL +R +L+L+ NLRP LY
Sbjct: 60 NLCRKADAVLLGAVGSPQYDNLDRPLRPERG-LLAIRKDLNLFANLRPAVLYPELANAST 118
Query: 117 GKNKI----DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT----RFGVERVIRFA 168
K +I DI+ VRE T +Y G +R E +E NT + R+ A
Sbjct: 119 LKPEIVAGLDILIVRELTGDIYFGEPRGIRV---LENGEREGYNTMKYSESEIRRIAHVA 175
Query: 169 FEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSP 227
F+ A++ KKV V KANVL +LW +F E+ +EY D+E H YVD AM+++R+P
Sbjct: 176 FQSAQKRS-KKVCSVGKANVLETT-ELWREIFEEIGKEYPDVELSHMYVDNAAMQLVRAP 233
Query: 228 ESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIAN 287
+ FDV+ T N+FGDIL+D + + G LG+ S +++ G++EP HGSAPDIAG+ AN
Sbjct: 234 KQFDVIATGNIFGDILSDEASMLTGSLGMLPSASLDENGKGLYEPSHGSAPDIAGQNKAN 293
Query: 288 PLAAILSASMMLEY 301
PLA ILS +M+L Y
Sbjct: 294 PLATILSLAMLLRY 307
>gb|AAG31381.1| (AF197449) 3-isopropylmaltate dehydrogenase [Buchnera aphidicola]
Length = 363
Score = 197 bits (495), Expect = 2e-49
Identities = 127/358 (35%), Positives = 193/358 (53%), Gaps = 16/358 (4%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K RIAV+PGDGIG EV+ E K+L L+ + E +E+ G + G LP +
Sbjct: 4 KYRIAVLPGDGIGPEVMREAYKILNILKNYFSLPIETREFNVGGAAIDQDGVALPKTTLL 63
Query: 62 EFKKFDAIYFGAIGDPRVKPGILERGI----LLKMRFELDLYVNLRPVKLYHP--RLTPL 115
+ DAI FG+ LL +R +L+ NLRP +LY L+PL
Sbjct: 64 GCENSDAILFGSXXXXXXXXXXXXXXXXXXXLLPLRKHFNLFGNLRPAQLYSELIHLSPL 123
Query: 116 KG---KNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYA 172
+ N +I+ +RE T G+Y G K E A I + + R+ AF+ A
Sbjct: 124 RSDIINNGFNILCIRELTGGIYFGKPAGRLKKNNIEYAFDTEIYYNYEINRIAHLAFQLA 183
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
K + KV +DK+NVL + LW + +VS+ Y D+ H Y+D M++I++P FD
Sbjct: 184 K-TRNYKVCSIDKSNVLN-SSILWREIVQKVSKNYPDVHLSHLYIDNATMQIIKNPNQFD 241
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
+++ N+FGDI++D A I G +G+ S ++N + G++EP GSAPDI GK IANP+A
Sbjct: 242 ILLCSNLFGDIISDECAMITGSIGMLPSASLNEKKFGLYEPAGGSAPDIEGKNIANPIAQ 301
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN---LKTYEVGDWVVKFL 345
ILS SM++ Y +NL+ + I+++V + + T D++ N LKT E+GD + L
Sbjct: 302 ILSVSMLVRYSMNLKTIADKIDQSVISVLKKGYRTADISNNSHYLKTNEMGDVIASAL 359
>sp|Q56268|LEU3_THIFE 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|478430|pir||JX0286 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - Thiobacillus ferrooxidans
>gi|3318965|pdb|1A05|A Chain A, Crystal Structure Of The
Complex Of 3-Isopropylmalate Dehydrogenase From
Thiobacillus Ferrooxidans With 3-Isopropylmalate
>gi|3318966|pdb|1A05|B Chain B, Crystal Structure Of The
Complex Of 3-Isopropylmalate Dehydrogenase From
Thiobacillus Ferrooxidans With 3-Isopropylmalate
>gi|414355|dbj|BAA03437.1| (D14585) 3-isopropylmalate
dehydrogenase [Thiobacillus ferrooxidans]
Length = 358
Score = 195 bits (491), Expect = 6e-49
Identities = 125/334 (37%), Positives = 188/334 (55%), Gaps = 12/334 (3%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
+IA+ GDGIG E+VA +VL +++ + + E G + + LP +++
Sbjct: 3 KIAIFAGDGIGPEIVAAARQVLDAVDQAAHLGLRCTEGLVGGAALDASDDPLPAASLQLA 62
Query: 64 KKFDAIYFGAIGDPR---VKPGILERGILLKMRFELDLYVNLRPVKLYHPRL--TPLKGK 118
DA+ GA+G PR P LL++R LDLY NLRP +++ L +PL+ +
Sbjct: 63 MAADAVILGAVGGPRWDAYPPAKRPEQGLLRLRKGLDLYANLRPAQIFPQLLDASPLRPE 122
Query: 119 --NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSG 176
+DI+ VRE T +Y G L + M+ + R+ AF A +
Sbjct: 123 LVRDVDILVVRELTGDIYFGQPRGLEVIDGKRRGFNTMVYDEDEIRRIAHVAFR-AAQGR 181
Query: 177 RKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVT 235
RK++ VDKANVL LW V EV+++Y D+ H YVD AM++IR+P FDV++T
Sbjct: 182 RKQLCSVDKANVLETTR-LWREVVTEVARDYPDVRLSHMYVDNAAMQLIRAPAQFDVLLT 240
Query: 236 PNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSA 295
NMFGDIL+D +++ G +G+ S ++ E M+EP+HGSAPDIAG+ ANPLA ILS
Sbjct: 241 GNMFGDILSDEASQLTGSIGMLPSASLG-EGRAMYEPIHGSAPDIAGQDKANPLATILSV 299
Query: 296 SMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDM 328
+MML + LN E ++ +E AV+R + + T D+
Sbjct: 300 AMMLRHSLNAEPWAQRVEAAVQRVLDQGLRTADI 333
>sp|Q01987|LEU3_CANBO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|323016|pir||A43324 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Candida boidinii)
>gi|170880|gb|AAA34349.1| (M98832) 3-isopropylmalate
dehydrogenase [Candida boidinii]
Length = 365
Score = 195 bits (490), Expect = 8e-49
Identities = 138/369 (37%), Positives = 205/369 (55%), Gaps = 36/369 (9%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELS-RVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I ++PGD +G EVV E +K+LK + E+ + F+F+ + G TG LPD A+E
Sbjct: 7 KIVLLPGDHVGVEVVEEAVKILKSISEVKPEIQFKFENHLIGGAAIDATGVPLPDEALEA 66
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
KK DA+ GA+G P+ G + E+G LLK+R EL+LY NLRP +L +PLK
Sbjct: 67 AKKSDAVLLGAVGGPKWGTGEVRPEQG-LLKIRKELNLYANLRPCNFASDKLLDLSPLKS 125
Query: 118 K--NKIDIVFVRENTEGLYAG------AGGFLRKGTPQEIAVQEMINTRFGVERVIRFA- 168
D VRE G+Y G GF + +V E VER+ R A
Sbjct: 126 DIVKGTDFTVVRELVGGIYFGDRVEDDGSGFASDS--ESYSVPE-------VERITRMAA 176
Query: 169 FEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY--DLETDHYYVDAMAMKMIRS 226
F + + +DKANVL + LW + V +E L H +D+ AM +++S
Sbjct: 177 FLSLQNDPPLPIWSLDKANVLASSR-LWRKTVDRVIKEEFPKLTVQHQLIDSAAMILVKS 235
Query: 227 PESFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDI 280
P + +V+T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+
Sbjct: 236 PTKLNGIVITNNMFGDIISDEASVIPGSLGLLPSASLASLPDTNQAFGLYEPCHGSAPDL 295
Query: 281 AGKRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGD 339
++ NP+A ILSA+MML+ LNL KE +EEAV++ + + +T D+ GN T EVGD
Sbjct: 296 PKNKV-NPIATILSAAMMLKLSLNLVKEGNAVEEAVRKVLDQGIMTGDLGGNNSTTEVGD 354
Query: 340 WVVKFLEVV 348
+ K ++++
Sbjct: 355 AIAKEVKLL 363
>pir||E71180 probable 3-isopropylmalate dehydrogenase - Pyrococcus horikoshii
>gi|3258153|dbj|BAA30836.1| (AP000007) 323aa long
hypothetical 3-isopropylmalate dehydrogenase [Pyrococcus
horikoshii]
Length = 323
Score = 192 bits (483), Expect = 5e-48
Identities = 115/306 (37%), Positives = 175/306 (56%), Gaps = 21/306 (6%)
Query: 37 EFKEYPFGAEHYLKTGETLPDWAIEEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFEL 96
+F E+ G + K G + + +EE +K DAI FGA P P + +++ +R EL
Sbjct: 10 KFYEFEGGLSVFKKYGVPIREEDLEEIRKMDAILFGATTTPFDVPRY--KSLIITLRKEL 67
Query: 97 DLYVNLRPVKLYHPRLTPLKGKNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMIN 156
DLY NLR + + K + +I+ VREN+EGLY+G G + V I
Sbjct: 68 DLYANLRIIPNF---------KLRKEIIIVRENSEGLYSGEGAY-----DSNKVVDFRII 113
Query: 157 TRFGVERVIRFAFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYV 216
TR G ER+ +FA + AK +T V KAN+L + + ++ +++++ D++ V
Sbjct: 114 TRKGAERIAKFAVKLAKDRSTF-LTFVHKANILE-SDRFFRKIVLDIARKEDVKVREEIV 171
Query: 217 DAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGS 276
D+ +K+++ P + ++++ NMFGDIL+DL G +GI SGN E +FEP+HGS
Sbjct: 172 DSFTIKLVKDPWNLGIILSENMFGDILSDLATIHAGSIGIVPSGNYG-EDIALFEPIHGS 230
Query: 277 APDIAGKRIANPLAAILSASMMLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYE 336
APDIAGK IANP+ AILSA+MML+YL L+ W +AV R + +TPDM G T E
Sbjct: 231 APDIAGKGIANPIGAILSAAMMLDYLGLDGSIIW--KAVGRYVRRGNLTPDMEGRATTLE 288
Query: 337 VGDWVV 342
V + ++
Sbjct: 289 VTNGII 294
>gb|AAB91428.1| (U86815) beta-isopropylmalate dehydrogenase [Expression vector
pESP-2]
Length = 364
Score = 192 bits (482), Expect = 7e-48
Identities = 136/358 (37%), Positives = 202/358 (55%), Gaps = 24/358 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I V+PGD +G+E+ AE +KVLK + ++ S V F+F+ + G TG LPD A+E
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPDEALEA 65
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P+K
Sbjct: 66 SKKVDAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKR 174
+ D V VRE G+Y G ++ +A T V+R+ R A F +
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKR---KEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQH 181
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEYD-LETDHYYVDAMAMKMIRSPESFD- 231
+ +DKANVL + LW + E + E+ L+ H +D+ AM ++++P +
Sbjct: 182 EPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNG 240
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRIA 286
+++T NMFGDI++D + I G LG+ S ++ P+ G++EP HGSAPD+ K A
Sbjct: 241 IIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLP-KNKA 299
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
NP+A ILSA+MML+ LNL +E K IE+AVK+ + T D+ G+ T EVGD V +
Sbjct: 300 NPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAE 357
>pir||T38621 probable isopropyl malate dehydrogenase - fission yeast
(Schizosaccharomyces pombe) >gi|2388955|emb|CAB11688.1|
(Z98979) putative homoisocitrate dehydrogenase; lysine
biosynthesis [Schizosaccharomyces pombe]
Length = 362
Score = 191 bits (480), Expect = 1e-47
Identities = 131/365 (35%), Positives = 198/365 (53%), Gaps = 37/365 (10%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
++ + +IP DGIGKEVV ++++ L ++ F+F + G + +TG+ LP+ +E
Sbjct: 6 RIVLGLIPADGIGKEVVPAARRLMENLPAKHKLKFDFIDLDAGWGTFERTGKALPERTVE 65
Query: 62 EFK-KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
K + +A FGA+ P K I+ +R ++ LY N+RPVK L KGK
Sbjct: 66 RLKTECNAALFGAVQSPTHKVAGYSSPIVA-LRKKMGLYANVRPVK----SLDGAKGK-P 119
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIA-----VQEMINTRFGVERVIRFAFEYAKR- 174
+D+V VRENTE LY ++ + +A + E +T+ G + AFE AK
Sbjct: 120 VDLVIVRENTECLYVKEERMVQNTPGKRVAEAIRRISEEASTKIG-----KMAFEIAKSR 174
Query: 175 ---------SGRKK--VTLVDKANVLTYAHDLWERVFAEVSQEYD-----LETDHYYVDA 218
S KK VT++ K+NV++ L+ R +Q D + D VD+
Sbjct: 175 QKIRESGTYSIHKKPLVTIIHKSNVMSVTDGLF-RESCRHAQSLDPSYASINVDEQIVDS 233
Query: 219 MAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAP 278
M ++ R PE FDVVV PN++GDIL+D A ++G LG+ S N+ + M EPVHGSAP
Sbjct: 234 MVYRLFREPECFDVVVAPNLYGDILSDGAASLIGSLGLVPSANVG-DNFVMSEPVHGSAP 292
Query: 279 DIAGKRIANPLAAILSASMMLEYLNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEV 337
DIAG+ IANP+A S ++MLE++ + + I AV + + E KV TPD+ G T E+
Sbjct: 293 DIAGRGIANPVATFRSVALMLEFMGHQDAAADIYTAVDKVLTEGKVLTPDLGGKSGTNEI 352
Query: 338 GDWVV 342
D V+
Sbjct: 353 TDAVL 357
>pir||DEBYI 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - yeast
(Saccharomyces cerevisiae) >gi|4698|emb|CAA27459.1|
(X03840) beta-isopropylmalate dehydrogenase
[Saccharomyces cerevisiae] >gi|1907128|emb|CAA42366.1|
(X59720) YCL018w, len:364 [Saccharomyces cerevisiae]
>gi|2689263|gb|AAB88909.1| (U67875) 3-isopropylmalate
dehydrogenase [Expression vector pESP-I]
Length = 364
Score = 190 bits (479), Expect = 1e-47
Identities = 134/358 (37%), Positives = 202/358 (55%), Gaps = 24/358 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I V+PGD +G+E+ AE +KVLK + ++ S V F+F+ + G TG LPD A+E
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPDEALEA 65
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P+K
Sbjct: 66 SKKVDAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKR 174
+ D V VRE G+Y G ++ +A T V+R+ R A F +
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKR---KEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQH 181
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEYD-LETDHYYVDAMAMKMIRSPESFD- 231
+ +DKANVL + LW + E + E+ L+ H +D+ AM ++++P +
Sbjct: 182 EPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNG 240
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRIA 286
+++T NMFGDI++D + I G LG+ S ++ P+ G++EP HGSAPD+ ++
Sbjct: 241 IIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKNKV- 299
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
NP+A ILSA+MML+ LNL +E K IE+AVK+ + T D+ G+ T EVGD V +
Sbjct: 300 NPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAE 357
>gb|AAB49959.1| (U70024) LEU2 [Cloning vector pGAD-C1] >gi|1595845|gb|AAB49962.1|
(U70025) LEU2 [Cloning vector pGAD-C2]
>gi|1595849|gb|AAB49965.1| (U70026) LEU2 [Cloning vector
pGAD-C3]
Length = 364
Score = 190 bits (479), Expect = 1e-47
Identities = 133/358 (37%), Positives = 203/358 (56%), Gaps = 24/358 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I V+PGD +G+E+ AE +KVLK + ++ S V F+F+ + G + TG LPD A+E
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAFEATGVPLPDEALEA 65
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P+K
Sbjct: 66 SKKVDAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKR 174
+ D V VRE G+Y G ++ +A T V+R+ R A F +
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKR---KEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQH 181
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEYD-LETDHYYVDAMAMKMIRSPESFD- 231
+ +DKANVL + LW + E + E+ L+ H +D+ AM ++++P +
Sbjct: 182 EPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNG 240
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRIA 286
+++T NMFGDI++D + I G LG+ S ++ P+ G++EP HGSAPD+ ++
Sbjct: 241 IIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKNKV- 299
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
+P+A ILSA+MML+ LNL +E K IE+AVK+ + T D+ G+ T EVGD V +
Sbjct: 300 DPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAE 357
>gi|10383772 beta-IPM (isopropylmalate) dehydrogenase; Leu2p [Saccharomyces
cerevisiae]
Length = 364
Score = 190 bits (478), Expect = 2e-47
Identities = 134/358 (37%), Positives = 202/358 (55%), Gaps = 24/358 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I V+PGD +G+E+ AE +KVLK + ++ S V F+F+ + G TG LPD A+E
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPDEALEA 65
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P+K
Sbjct: 66 SKKADAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKR 174
+ D V VRE G+Y G ++ +A T V+R+ R A F +
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKR---KEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQH 181
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEYD-LETDHYYVDAMAMKMIRSPESFD- 231
+ +DKANVL + LW + E + E+ L+ H +D+ AM ++++P +
Sbjct: 182 EPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNG 240
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRIA 286
+++T NMFGDI++D + I G LG+ S ++ P+ G++EP HGSAPD+ ++
Sbjct: 241 IIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKNKV- 299
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
NP+A ILSA+MML+ LNL +E K IE+AVK+ + T D+ G+ T EVGD V +
Sbjct: 300 NPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAE 357
>sp|P41926|LEU3_YAMOH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1085606|pir||S50698 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Pichia ohmeri)
>gi|510934|emb|CAA84484.1| (Z35101) 3-isopropylmalate
dehydrogenase [Kodamaea ohmeri]
Length = 368
Score = 190 bits (477), Expect = 3e-47
Identities = 136/368 (36%), Positives = 202/368 (53%), Gaps = 28/368 (7%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
I V+PGD +G E+ E +KVLK +E++S V FEFK + G TG LPD +E K
Sbjct: 6 ITVVPGDHVGTEICDEAIKVLKAIEKVSPVKFEFKHHLIGGAAIDATGVPLPDETLEAAK 65
Query: 65 KFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHP---RLTPLKGK- 118
DA+ GA+G P+ G + E+G LLK+R EL+LY NLRP L+PLK +
Sbjct: 66 SSDAVLLGAVGGPKWGTGAVRPEQG-LLKIRKELNLYANLRPCNFVSDSLLELSPLKSEI 124
Query: 119 -NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINT-RFGVERVIRF----AFEYA 172
+ VRE G+Y G R+ + Q +T ++ VE V R AF
Sbjct: 125 VKGTNFTVVRELVGGIYFGE----RQEQEESSDGQSAWDTEKYSVEEVTRITRMAAFMAL 180
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEV-SQEY-DLETDHYYVDAMAMKMIRSPESF 230
+ + + +DKANVL + LW + V S E+ L+ H +D+ AM +++SP
Sbjct: 181 QHNPPLPIWSLDKANVLALSR-LWRKTVDHVMSTEFPQLKVQHQLIDSAAMILVKSPSQL 239
Query: 231 D-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKR 284
+ V++T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+ +
Sbjct: 240 NGVIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNSAFGLYEPCHGSAPDLPANK 299
Query: 285 IANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
+ NP+A ILS +MML L+ KE++ +E+AV + + T D+ G+ T EVGD V
Sbjct: 300 V-NPVATILSVAMMLRLSLDCLKEAEALEKAVGQVLDAGIRTADLRGSSSTKEVGDAVAA 358
Query: 344 FLEVVANE 351
+E + E
Sbjct: 359 AVEKILAE 366
>emb|CAA60637.1| (X87172) NAD+-isocitrate dehydrogenase, alpha subunit [Macaca
fascicularis]
Length = 340
Score = 190 bits (477), Expect = 3e-47
Identities = 121/331 (36%), Positives = 180/331 (53%), Gaps = 16/331 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E+ A +K+ + ++ +++E A +P A E
Sbjct: 8 VTLIPGDGIGPEISAAVMKIF----DAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMD 63
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
K G + P + G +LL+ F DLY N+RP TP ++IV
Sbjct: 64 KNKMGLKGPLKTP-IAAGHPSMNLLLRKTF--DLYANVRPCVSIEGYKTPY---TDVNIV 117
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RENTEG Y+G + G Q I + T G +R+ FAFEYA+ + R VT V
Sbjct: 118 TIRENTEGEYSGIEHVIVDGVVQSIK----LITEGGSKRIAEFAFEYARNNHRSNVTAVH 173
Query: 185 KANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ + L+ + EV++ D++ + Y+D + + M++ P FDV+V PN++GDIL
Sbjct: 174 KANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDIL 233
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+DL A ++GGLG+ SGNI +FE VHG+APDIAGK +ANP A +LSA MML ++
Sbjct: 234 SDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMG 293
Query: 304 LEKESKWIEEAVKRAIAENK-VTPDMNGNLK 333
L + IE A I + K +T D+ GN K
Sbjct: 294 LFDHAARIEAACFATIKDGKSLTKDLGGNAK 324
>sp|Q28480|IDHA_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|2117495|pir||S58664 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) alpha chain - crab-eating macaque
(fragment)
Length = 347
Score = 190 bits (477), Expect = 3e-47
Identities = 121/331 (36%), Positives = 180/331 (53%), Gaps = 16/331 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E+ A +K+ + ++ +++E A +P A E
Sbjct: 15 VTLIPGDGIGPEISAAVMKIF----DAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMD 70
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
K G + P + G +LL+ F DLY N+RP TP ++IV
Sbjct: 71 KNKMGLKGPLKTP-IAAGHPSMNLLLRKTF--DLYANVRPCVSIEGYKTPY---TDVNIV 124
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RENTEG Y+G + G Q I + T G +R+ FAFEYA+ + R VT V
Sbjct: 125 TIRENTEGEYSGIEHVIVDGVVQSIK----LITEGGSKRIAEFAFEYARNNHRSNVTAVH 180
Query: 185 KANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ + L+ + EV++ D++ + Y+D + + M++ P FDV+V PN++GDIL
Sbjct: 181 KANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDIL 240
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+DL A ++GGLG+ SGNI +FE VHG+APDIAGK +ANP A +LSA MML ++
Sbjct: 241 SDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMG 300
Query: 304 LEKESKWIEEAVKRAIAENK-VTPDMNGNLK 333
L + IE A I + K +T D+ GN K
Sbjct: 301 LFDHAARIEAACFATIKDGKSLTKDLGGNAK 331
>sp|P04173|LEU3_YEAST 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|171837|gb|AAA93044.1| (J01333) beta-IPM
dehydrogenase [Saccharomyces cerevisiae]
>gi|682656|gb|AAA74942.1| (L25833) 3-isopropylmalate
dehydrogenase [Cloning vector pYEULCBX]
>gi|915410|gb|AAB39920.1| (U29899) LEU2 [Cloning vector
pACT2] >gi|1000354|gb|AAA76716.1| (U35112) Leu2p
[synthetic construct] >gi|1049257|gb|AAA80348.1|
(U36450) 3-isopropylmalate dehydrogenase [Shuttle vector
pAD-GAL4] >gi|2981189|gb|AAC78773.1| (AF049063)
beta-isopropylmalate dehydrogenase [Expression vector
pYZ1N] >gi|2981194|gb|AAC78777.1| (AF049064)
beta-isopropylmalate dehydrogenase [Expression vector
pYZ3N-GFP] >gi|3136320|gb|AAC78830.1| (AF064066)
beta-isopropylmalate dehydrogenase [Expression vector
pYZ41N] >gi|3136324|gb|AAC78833.1| (AF064067)
beta-isopropylmalate dehydrogenase [Expression vector
pYZ81N] >gi|3243015|gb|AAC23871.1| (AF069719)
isopropylmalate dehydrogenase [Expression vector
pBEVY-GL] >gi|3243025|gb|AAC23878.1| (AF069722)
isopropylmalate dehydrogenase [Expression vector
pBEVY-L] >gi|6642850|gb|AAF20325.1| (AF134167)
isopropylmalate dehydrogenase [Yeast 2-hybrid vector
pC-ACT.1] >gi|6642854|gb|AAF20328.1| (AF134168)
isopropylmalate dehydrogenase [Yeast 2-hybrid vector
pC-ACT.2]
Length = 364
Score = 189 bits (474), Expect = 6e-47
Identities = 133/358 (37%), Positives = 202/358 (56%), Gaps = 24/358 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I V+PGD +G+E+ AE +KVLK + ++ S V F+F+ + G TG LPD A+E
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPDEALEA 65
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P+K
Sbjct: 66 SKKVDAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKR 174
+ D V VRE G+Y G ++ +A T V+R+ R A F +
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKR---KEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQH 181
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEYD-LETDHYYVDAMAMKMIRSPESFD- 231
+ +DKANVL + LW + E + E+ L+ H +D+ AM ++++P +
Sbjct: 182 EPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNG 240
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRIA 286
+++T NMFGDI++D + I G LG+ S ++ P+ G++EP HGSAPD+ ++
Sbjct: 241 IIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKNKV- 299
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
+P+A ILSA+MML+ LNL +E K IE+AVK+ + T D+ G+ T EVGD V +
Sbjct: 300 DPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAE 357
>gb|AAD56647.1|AF178973_2 (AF178973) dLEU2 [Cloning vector pCS512]
>gi|5929926|gb|AAD56650.1|AF178973_5 (AF178973) LEU2
[Cloning vector pCS512]
>gi|6318857|gb|AAF07046.1|AF178582_1 (AF178582) LEU2p
[Expression vector pCS316]
>gi|6318865|gb|AAF07053.1|AF178583_3 (AF178583) dLEU2
[Expression vector pSB229]
>gi|6457291|gb|AAF09455.1|AF179625_1 (AF179625) dLEU2
[Shuttle vector pCS513]
>gi|6457295|gb|AAF09459.1|AF179625_5 (AF179625) LEU2
[Shuttle vector pCS513]
>gi|6457299|gb|AAF09462.1|AF179626_3 (AF179626) dLEU2
[Expression vector pGP100]
>gi|6457310|gb|AAF09471.1|AF179628_4 (AF179628) dLEU2
[Shuttle vector pHIGEXhOR]
Length = 368
Score = 189 bits (474), Expect = 6e-47
Identities = 133/358 (37%), Positives = 202/358 (56%), Gaps = 24/358 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I V+PGD +G+E+ AE +KVLK + ++ S V F+F+ + G TG LPD A+E
Sbjct: 10 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPDEALEA 69
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P+K
Sbjct: 70 SKKVDAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELQLYANLRPCNFASDSLLDLSPIKP 128
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKR 174
+ D V VRE G+Y G ++ +A T V+R+ R A F +
Sbjct: 129 QFAKGTDFVVVRELVGGIYFGKR---KEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQH 185
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEYD-LETDHYYVDAMAMKMIRSPESFD- 231
+ +DKANVL + LW + E + E+ L+ H +D+ AM ++++P +
Sbjct: 186 EPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNG 244
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRIA 286
+++T NMFGDI++D + I G LG+ S ++ P+ G++EP HGSAPD+ ++
Sbjct: 245 IIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKNKV- 303
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
+P+A ILSA+MML+ LNL +E K IE+AVK+ + T D+ G+ T EVGD V +
Sbjct: 304 DPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAE 361
>sp|P07139|LEU3_CANMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1076952|pir||S48228 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) (allele LS) - yeast (Candida
maltosa) >gi|2606|emb|CAA29024.1| (X05459) C-Leu 2 (AA
1-373) [Candida maltosa] >gi|296354|emb|CAA51445.1|
(X72940) 3-isopropylmalate dehydrogenase [Candida
maltosa]
Length = 373
Score = 188 bits (473), Expect = 7e-47
Identities = 133/371 (35%), Positives = 204/371 (54%), Gaps = 29/371 (7%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELS---RVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
I ++PGD +G E+V E +KVL+ +E + ++ F+FK + G TG LPD A+E
Sbjct: 8 ITILPGDHVGTEIVNEAIKVLEAIEAATPYQKIHFDFKHHLIGGAAIDATGVPLPDDALE 67
Query: 62 EFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRLTPL---- 115
K DA+ GA+G P+ G L E+G LLK+R EL+LY N+RP L L
Sbjct: 68 SAKNSDAVLLGAVGGPKWGTGALRPEQG-LLKIRKELNLYANIRPCNFASDSLLELSPLR 126
Query: 116 ----KGKNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FE 170
KG N ++ VRE G+Y G + ++ A T V R+ R A F
Sbjct: 127 PEVVKGTN---LIIVRELVGGIYFGDREEQEESADKQTAWDTEKYTVDEVTRITRMAAFM 183
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEV-SQEYD-LETDHYYVDAMAMKMIRSPE 228
+ + + +DKANVL + LW R +V S+E+ L H +D+ AM +I++P
Sbjct: 184 ALQHTPPLPIWSLDKANVLASSR-LWRRTVDKVISEEFPTLSVQHQLIDSAAMILIQNPT 242
Query: 229 SFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAG 282
+ +++T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+
Sbjct: 243 KLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNTAFGLYEPCHGSAPDLPA 302
Query: 283 KRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
++ NP+A ILSA+ ML L+ KE++ +EEAVK+ + + T D+ G T EVGD +
Sbjct: 303 NKV-NPIATILSAASMLRLSLDCVKEAEALEEAVKQVLDKGIRTADLRGTSSTTEVGDAI 361
Query: 342 VKFLEVVANEE 352
V+ + + E+
Sbjct: 362 VEAVTKILKEK 372
>sp|Q00412|LEU3_SPIPL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|152903|gb|AAA26593.1| (M75903)
3-isopropylmalate dehydrogenase [Spirulina platensis]
Length = 355
Score = 188 bits (473), Expect = 7e-47
Identities = 131/350 (37%), Positives = 189/350 (53%), Gaps = 17/350 (4%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
RI ++ GDGIG E++A + VLK + + ++FEFKE G TGE LP+ +++
Sbjct: 6 RITLLSGDGIGPEIMAVAVDVLKAVGKQLDLNFEFKEALMGGVAIDATGEPLPEESLQAC 65
Query: 64 KKFDAIYFGAIG----DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN 119
+ DA+ AIG D +P E G LL +R L L+ N R L+ P +
Sbjct: 66 RDSDAVLLAAIGGYKWDNLPRPERPETG-LLALRAGLGLFAN-RARLLFCPHVLDASSLK 123
Query: 120 K-----IDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAK 173
+ +DI+ VRE T G+Y G G T + M ++R+ R FE AK
Sbjct: 124 REVVEGVDIMVVRELTGGIYFGQPKGIFETETGKNEGSNTMAYGESEIDRIGRVGFETAK 183
Query: 174 -RSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDV 232
R GR + VDKANVL + +R+ A + ++E H YVD AM+++R P+ FD
Sbjct: 184 KRQGR--LCSVDKANVLDVSQLWRDRIMALAADYPEVELSHLYVDNAAMQLVRWPKQFDT 241
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAI 292
+VT N+FGDIL+D A + G +G+ S ++ G+FEPVHGSAPDIAG+ ANPLA +
Sbjct: 242 IVTGNLFGDILSDAAAMLTGSIGMLPSASLGASGPGVFEPVHGSAPDIAGQDKANPLAQV 301
Query: 293 LSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPD-MNGNLKTYEVGDW 340
LSA+MML Y L+ S +E+AV + + T D M+ +K W
Sbjct: 302 LSAAMMLRYGLDEPAASDPVEKAVLKVLDWGYPTGDIMSEGMKAVGCRKW 351
>prf||1401173A C-LEU2 gene [Candida maltosa]
Length = 373
Score = 188 bits (473), Expect = 7e-47
Identities = 133/371 (35%), Positives = 204/371 (54%), Gaps = 29/371 (7%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELS---RVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
I ++PGD +G E+V E +KVL+ +E + ++ F+FK + G TG LPD A+E
Sbjct: 8 ITILPGDHVGTEIVNEAIKVLEAIEAATPYQKIHFDFKHHLIGGAAIDATGVPLPDDALE 67
Query: 62 EFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRLTPL---- 115
K DA+ GA+G P+ G L E+G LLK+R EL+LY N+RP L L
Sbjct: 68 SAKNSDAVLLGAVGGPKWGTGALRPEQG-LLKIRKELNLYANIRPCNFASDSLLELSPLR 126
Query: 116 ----KGKNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FE 170
KG N ++ VRE G+Y G + ++ A T V R+ R A F
Sbjct: 127 PEVVKGTN---LIIVRELVGGIYFGDREEQEESADKQTAWDTEKYTVDEVTRITRMAAFM 183
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEV-SQEYD-LETDHYYVDAMAMKMIRSPE 228
+ + + +DKANVL + LW R +V S+E+ L H +D+ AM +I++P
Sbjct: 184 ALQHTPPLPIWSLDKANVLASSR-LWRRTVDKVISEEFPTLSVQHQLIDSAAMILIQNPT 242
Query: 229 SFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAG 282
+ +++T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+
Sbjct: 243 KLNGIIITSNMFGDIISDEASVIQGSLGLLPSASLASLPDTNTAFGLYEPCHGSAPDLPA 302
Query: 283 KRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
++ NP+A ILSA+ ML L+ KE++ +EEAVK+ + + T D+ G T EVGD +
Sbjct: 303 NKV-NPIATILSAASMLRLSLDCVKEAEALEEAVKQVLDKGIRTADLRGTSSTTEVGDAI 361
Query: 342 VKFLEVVANEE 352
V+ + + E+
Sbjct: 362 VEAVTKILKEK 372
>pir||S48226 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (allele LA) - yeast
(Candida maltosa) >gi|296348|emb|CAA51444.1| (X72939)
3-isopropylmalate dehydrogenase [Candida maltosa]
Length = 373
Score = 188 bits (473), Expect = 7e-47
Identities = 134/371 (36%), Positives = 205/371 (55%), Gaps = 29/371 (7%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELS---RVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
I ++PGD +G E+V E +KVL+ +E + ++ F+FK + G TG LPD A+E
Sbjct: 8 ITILPGDHVGTEIVNEAIKVLEAIEAATPYQKIHFDFKHHLIGGAAIDATGVPLPDDALE 67
Query: 62 EFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRLTPL---- 115
KK DA+ GA+G P+ G L E+G LLK+R EL+LY N+RP L L
Sbjct: 68 SAKKSDAVLLGAVGGPKWWTGALRPEQG-LLKIRKELNLYANIRPCNFASDSLLELSPLR 126
Query: 116 ----KGKNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FE 170
KG N ++ VRE G+Y G + ++ A T V R+ R A F
Sbjct: 127 PEVVKGTN---LIIVRELVGGIYFGDREEQEESEDKQTAWDTEKYTVDEVTRITRMAAFM 183
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEV-SQEYD-LETDHYYVDAMAMKMIRSPE 228
+ + + +DKANVL + LW R +V S+E+ L H +D+ AM +I++P
Sbjct: 184 PLQDNPPLPIWSLDKANVLASSR-LWRRTVDKVISEEFPTLSVQHQLIDSAAMILIQNPT 242
Query: 229 SFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAG 282
+ +++T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+
Sbjct: 243 KLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNTAFGLYEPCHGSAPDLPA 302
Query: 283 KRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
++ NP+A ILSA+ ML L+ KE++ +EEAVK+ + + T D+ G T EVGD +
Sbjct: 303 NKV-NPIATILSAASMLRLSLDCVKEAEALEEAVKQVLDKGIRTADLRGTRSTTEVGDAI 361
Query: 342 VKFLEVVANEE 352
V+ + + E+
Sbjct: 362 VEAVTKILKEK 372
>pir||S48225 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (allele LEa) - yeast
(Candida maltosa) >gi|296350|emb|CAA51442.1| (X72937)
3-isopropylmalate dehydrogenase [Candida maltosa]
Length = 373
Score = 188 bits (473), Expect = 7e-47
Identities = 133/371 (35%), Positives = 204/371 (54%), Gaps = 29/371 (7%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELS---RVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
I ++PGD +G E+V E +KVL+ +E + ++ F+FK + G TG LPD A+E
Sbjct: 8 ITILPGDHVGTEIVNEAIKVLEAIEAATPYQKIHFDFKHHLIGGSAIDATGVPLPDDALE 67
Query: 62 EFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRLTPL---- 115
K DA+ GA+G P+ G L E+G LLK+R EL+LY N+RP L L
Sbjct: 68 SAKNSDAVLLGAVGGPKWGTGALRPEQG-LLKIRKELNLYANIRPCNFASDSLLELSPLR 126
Query: 116 ----KGKNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FE 170
KG N ++ VRE G+Y G + ++ A T V R+ R A F
Sbjct: 127 PEVVKGTN---LIIVRELVGGIYFGDREEQEESEDKQTAWDTEKYTVDEVTRITRIAAFM 183
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEV-SQEYD-LETDHYYVDAMAMKMIRSPE 228
+ + + +DKANVL + LW R +V S+E+ L H +D+ AM +I++P
Sbjct: 184 ALQHNPPLPIWSLDKANVLASSR-LWRRTVDKVISEEFPTLSVQHQLIDSAAMILIQNPT 242
Query: 229 SFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAG 282
+ +++T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+
Sbjct: 243 KLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNTVFGLYEPCHGSAPDLPA 302
Query: 283 KRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
++ NP+A ILSA+ ML L+ KE++ +EEAVK+ + + T D+ G T EVGD +
Sbjct: 303 NKV-NPIATILSAASMLRLSLDCVKEAEALEEAVKQVLDKGIRTADLRGTSSTTEVGDAI 361
Query: 342 VKFLEVVANEE 352
V+ + + E+
Sbjct: 362 VEAVTKILKEK 372
>pir||A44851 3-isopropylmalate dehydrogenase (EC 1.1.1.85) - Spirulina platensis
Length = 355
Score = 188 bits (473), Expect = 7e-47
Identities = 131/350 (37%), Positives = 189/350 (53%), Gaps = 17/350 (4%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
RI ++ GDGIG E++A + VLK + + ++FEFKE G TGE LP+ +++
Sbjct: 6 RITLLSGDGIGPEIMAVAVDVLKAVGKQLDLNFEFKEALMGGVAIDATGEPLPEESLQAC 65
Query: 64 KKFDAIYFGAIG----DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN 119
+ DA+ AIG D +P E G LL +R L L+ N R L+ P +
Sbjct: 66 RDSDAVLLAAIGGYKWDNLPRPERPETG-LLALRAGLGLFAN-RARLLFCPHVLDASSLK 123
Query: 120 K-----IDIVFVRENTEGLYAGAG-GFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAK 173
+ +DI+ VRE T G+Y G G T + M ++R+ R FE AK
Sbjct: 124 REVVEGVDIMVVRELTGGIYFGQPKGIFETETGKNEGSNTMAYGESEIDRIGRVGFETAK 183
Query: 174 -RSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYDLETDHYYVDAMAMKMIRSPESFDV 232
R GR + VDKANVL + +R+ A + ++E H YVD AM+++R P+ FD
Sbjct: 184 KRQGR--LCSVDKANVLDVSQLWRDRIMALAADYPEVELSHLYVDNAAMQLVRWPKQFDT 241
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAI 292
+VT N+FGDIL+D A + G +G+ S ++ G+FEPVHGSAPDIAG+ ANPLA +
Sbjct: 242 IVTGNLFGDILSDAAAMLTGSIGMLPSASLGASPGGVFEPVHGSAPDIAGQDKANPLAQV 301
Query: 293 LSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPD-MNGNLKTYEVGDW 340
LSA+MML Y L+ S +E+AV + + T D M+ +K W
Sbjct: 302 LSAAMMLRYGLDEPAASDPVEKAVLKVLDWGYPTGDIMSEGMKAVGCRKW 351
>sp|P41563|IDHA_BOVIN ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
(SUBUNITS 3/4) >gi|2117494|pir||S58435 isocitrate
dehydrogenase (NAD+) (EC 1.1.1.41) chain 3/4 precursor -
bovine >gi|902743|gb|AAC18425.1| (U07980) NAD+-dependent
isocitrate dehydrogenase [Bos taurus]
Length = 366
Score = 188 bits (473), Expect = 7e-47
Identities = 120/331 (36%), Positives = 180/331 (54%), Gaps = 16/331 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E+ A +K+ + ++ +++E A +P A E
Sbjct: 34 VTLIPGDGIGPEISAAVMKIF----DAAKAPIQWEERNVAAIQGPGGKWMIPPEAKESMD 89
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
K G + P + G +LL+ F DLY N+RP TP + ++IV
Sbjct: 90 KNKMGLKGPLKTP-IAAGHPSMNLLLRKTF--DLYANVRPCVSIEGYKTPY---HDVNIV 143
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RENTEG Y+G + G Q I + T +R+ FAFEYA+ + R VT V
Sbjct: 144 TIRENTEGEYSGIEHVIVDGVVQSIK----LITEAASKRIAEFAFEYARNNHRSNVTAVH 199
Query: 185 KANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ + L+ + EV++ D++ + Y+D + + M++ P FDV+V PN++GDIL
Sbjct: 200 KANIMRMSDGLFLQKCREVAENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDIL 259
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+DL A ++GGLG+ SGNI +FE VHG+APDIAGK +ANP A +LSA MML ++
Sbjct: 260 SDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMG 319
Query: 304 LEKESKWIEEAVKRAIAENK-VTPDMNGNLK 333
L + IE A I + K +T D+ GN K
Sbjct: 320 LFDHAAKIETACFATIKDGKSLTKDLGGNSK 350
>sp|P23390|LEU3_KLULA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|283086|pir||S25369 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Kluyveromyces
marxianus var. lactis) >gi|2855|emb|CAA46514.1| (X65545)
3-isopropylmalate dehydrogenase [Kluyveromyces lactis]
>gi|11344907|gb|AAG34541.1| (AF298792)
beta-isopropylmalate dehydrogenase [PCR template vector
pJJH727]
Length = 362
Score = 187 bits (470), Expect = 2e-46
Identities = 135/366 (36%), Positives = 202/366 (54%), Gaps = 24/366 (6%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELS-RVSFEFKEYPFGAEHYLKTGETLPDWA 59
M I V+PGD +GKEV E +KVL + E+ + F F+ + G TG LPD A
Sbjct: 1 MSKNIVVLPGDHVGKEVTDEAIKVLNAIAEVRPEIKFNFQHHLIGGAAIDATGTPLPDEA 60
Query: 60 IEEFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TP 114
+E KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P
Sbjct: 61 LEASKKADAVLLGAVGGPKWGTGAVRPEQG-LLKIRKELGLYANLRPCNFASDSLLDLSP 119
Query: 115 LKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEY 171
LK + D V VRE G+Y G ++ +A + V+R+ R A F
Sbjct: 120 LKPEYAKGTDFVVVRELVGGIYFGER---KEDEGDGVAWDSEKYSVPEVQRITRMAAFLA 176
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEY-DLETDHYYVDAMAMKMIRSPES 229
+++ + +DKANVL + LW + E + E+ L H +D+ AM +++SP
Sbjct: 177 LQQNPPLPIWSLDKANVLASSR-LWRKTVEETIKTEFPQLTVQHQLIDSAAMILVKSPTK 235
Query: 230 FD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGK 283
+ VV+T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+
Sbjct: 236 LNGVVITNNMFGDIISDEASVIPGSLGLLPSASLASLPDTNKAFGLYEPCHGSAPDLPAN 295
Query: 284 RIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVV 342
++ NP+A ILSA+MML+ L+L +E + +EEAV+ + T D+ G+ T EVGD +
Sbjct: 296 KV-NPIATILSAAMMLKLSLDLVEEGRALEEAVRNVLDAGVRTGDLGGSNSTTEVGDAIA 354
Query: 343 KFLEVV 348
K ++ +
Sbjct: 355 KAVKEI 360
>emb|CAB72262.1| (AJ271154) 3-isopropylmalate dehydrogenase [Phaeosphaeria nodorum]
Length = 365
Score = 187 bits (470), Expect = 2e-46
Identities = 140/366 (38%), Positives = 201/366 (54%), Gaps = 31/366 (8%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
I V+ GDG+G EVVAE K+LK +E+ + +F F+E+ FG L D A+ K
Sbjct: 6 IVVLAGDGVGPEVVAEAFKILKVVEKHTDNTFNFQEHLFGGCSIDAHNNPLTDEALAAAK 65
Query: 65 KFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKGKN 119
DAI GA+G P+ G + E+GI LK+R EL + NLRP L +PLK +N
Sbjct: 66 GADAIILGAVGGPKWGTGKVRPEQGI-LKLRKELGTFGNLRPCFFASDSLVDSSPLKAEN 124
Query: 120 KIDIVF--VRENTEGLYAGA----GGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAK 173
+ F +RE T G+Y G G + + +V+E +ERV+R A A
Sbjct: 125 VRGVNFNIIRELTGGIYFGERKEDEGDGKAMDTEPYSVEE-------IERVVRLAGSLAS 177
Query: 174 RSGRK-KVTLVDKANVLTYAHDLWERVFAEV-SQEY-DLETDHYYVDAMAMKMIRSPESF 230
V +DKANVL + LW + F + EY L++ + +D+ AM MI++P +
Sbjct: 178 AENPPVPVWSLDKANVLATSR-LWRKTFERIMKDEYPHLKSGTHLIDSAAMLMIKNPRAL 236
Query: 231 D-VVVTPNMFGDILTDLGAEIVGGLGIAASGNI------NPETTGMFEPVHGSAPDIAGK 283
+ VVVT N+FGDI++D + I G LG+ S ++ + G++EP+HGSAPDIAG+
Sbjct: 237 NGVVVTSNLFGDIISDEASVIPGSLGLLPSASLGGIPDGKTKLNGIYEPIHGSAPDIAGQ 296
Query: 284 RIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVV 342
I NP+A ILS SMM +Y L L + + I+EA K I + T D+ G KT EVGD +
Sbjct: 297 GIVNPIATILSISMMFKYSLCLPELALKIDEATKITIDKGVRTGDIGGTSKTAEVGDAIA 356
Query: 343 KFLEVV 348
LE +
Sbjct: 357 AELETL 362
>sp|P08791|LEU3_PICJA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|482489|pir||A47620 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Pichia jadinii)
>gi|170874|gb|AAA34347.1| (M16014) 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) [Candida utilis]
Length = 363
Score = 187 bits (469), Expect = 2e-46
Identities = 132/362 (36%), Positives = 201/362 (55%), Gaps = 24/362 (6%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELS-RVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
I V+PGD +G E+ AE +KVLK +EE+ + F F+ + G TG LPD A+E
Sbjct: 6 IVVLPGDHVGTEITAEAIKVLKAIEEVKPEIKFNFQHHLIGGAAIDATGVPLPDDALEAS 65
Query: 64 KKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKGK 118
KK DA+ GA+G P+ G + E+G LLK+R EL+LY NLRP L +P+K +
Sbjct: 66 KKADAVLLGAVGGPKWGTGAVRPEQG-LLKIRKELNLYANLRPCNFASESLLDLSPIKAE 124
Query: 119 --NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKRS 175
D V VRE G+Y G ++ +A + V+R+ R A F + +
Sbjct: 125 VVKGTDFVVVRELVGGIYFGER---KEDDGSGVASDTETYSVPEVQRITRMAAFMALQHN 181
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAE-VSQEY-DLETDHYYVDAMAMKMIRSPESFD-V 232
+ +DKANVL + LW +V E + +E+ L H +D+ AM +I+ P + +
Sbjct: 182 PPLPIWSLDKANVLASSR-LWRKVVTETIEKEFPQLTVQHQLIDSAAMILIKYPTQLNGI 240
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNI-----NPETTGMFEPVHGSAPDIAGKRIAN 287
V+T NMFGDI++D + I G LG+ S ++ + + G++EP HGSAPD+ ++ N
Sbjct: 241 VITSNMFGDIISDEASVIPGSLGLLPSASLASLPDSNKAFGLYEPCHGSAPDLPANKV-N 299
Query: 288 PLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLE 346
P+A ILSA+MML+ L+L +E +E AVK+ + T D+ G T EVGD V + ++
Sbjct: 300 PIATILSAAMMLKLSLDLYEEGVAVETAVKQVLDAGIRTGDLKGTNSTTEVGDAVAEAVK 359
Query: 347 VV 348
+
Sbjct: 360 KI 361
>emb|CAB99456.1| (AJ292544) beta-isopropylmalate dehydrogenase [Zygosaccharomyces
bailii]
Length = 362
Score = 186 bits (468), Expect = 3e-46
Identities = 131/366 (35%), Positives = 203/366 (54%), Gaps = 24/366 (6%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELS-RVSFEFKEYPFGAEHYLKTGETLPDWA 59
M I V+PGD +G+E+ E +KVL+ + E+S + F F+ + G TG LPD A
Sbjct: 1 MSKNIVVLPGDHVGQEIAQEAIKVLEAISEVSPKAKFNFQHHLIGGSAIDATGSPLPDEA 60
Query: 60 IEEFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHP---RLTP 114
+ KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L+P
Sbjct: 61 LAAAKKADAVLLGAVGGPKWGTGAVRPEQG-LLKIRKELQLYANLRPCNFASESLLELSP 119
Query: 115 LKGKNK--IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEY 171
LK ++ D + VRE G+Y G ++ +A T+ V+R+ R A F
Sbjct: 120 LKPQHARGTDFIVVRELVGGIYFGQR---KEDDGDGVAWDSERYTKPEVQRITRMAAFLA 176
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEY-DLETDHYYVDAMAMKMIRSPES 229
+ + + +DKANVL + LW + E + E+ L H +D+ AM +I++P
Sbjct: 177 LQHNPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPQLTVKHQLIDSAAMILIKNPTQ 235
Query: 230 FD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGK 283
+ +++T NMF DI++D + I G LG+ S ++ P+T G++EP HGSAPD+
Sbjct: 236 LNGIIITSNMFEDIISDEASVIPGSLGLLPSASLASLPDTNEAFGLYEPCHGSAPDLPKG 295
Query: 284 RIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVV 342
++ NP+A ILSA+MML+ LN+ KE + +E AVK+ + T D+ G+ T EVGD +
Sbjct: 296 KV-NPVAMILSAAMMLKLSLNMAKEGEALETAVKQVLDSGVRTGDLGGSNSTSEVGDAIA 354
Query: 343 KFLEVV 348
K ++ +
Sbjct: 355 KAVKQI 360
>emb|CAC08508.1| (AJ294714) beta-isopropylmalate dehydrogenase [Pichia anomala]
Length = 364
Score = 186 bits (467), Expect = 4e-46
Identities = 133/357 (37%), Positives = 199/357 (55%), Gaps = 24/357 (6%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELS-RVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
I V+PGD +G E+ E +KVLK +EE+ V F F+ + G TG LPD A+E
Sbjct: 6 IVVLPGDHVGTEITNEAIKVLKAIEEVRPNVKFNFQHHLIGGAAIDATGVPLPDEALEAA 65
Query: 64 KKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHP---RLTPLKGK 118
KK DA+ GA+G P+ G + E+G LLK+R EL+LY NLRP L+P+K +
Sbjct: 66 KKADAVLLGAVGGPKWGTGAVRPEQG-LLKIRKELNLYANLRPCNFASESLLELSPIKAE 124
Query: 119 --NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKRS 175
D VRE G+Y G ++ +A + V+R+ R A F + +
Sbjct: 125 FAKGTDFTVVRELVGGIYFGER---KEDDGSGVASDTETYSVPEVQRITRMAAFMALQHN 181
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAE-VSQEY-DLETDHYYVDAMAMKMIRSPESFD-V 232
+ +DKANVL + LW + E + +E+ L H +D+ AM +I+SP + + +
Sbjct: 182 PPLPIWSLDKANVLASSR-LWRKTVTETIEKEFPQLTVQHQLIDSAAMILIKSPTTLNGI 240
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRIAN 287
V+T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+ ++ N
Sbjct: 241 VITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNKAFGLYEPCHGSAPDLPANKV-N 299
Query: 288 PLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
P+A ILSA+MML+ L+L +E +E+AVK + T D+ G+ T EVGD V+
Sbjct: 300 PIATILSAAMMLKLSLDLFEEGVALEKAVKEVLDAGVRTGDLRGSNSTKEVGDAAVE 356
>gb|AAA62618.1| (L14756) 3-isopropylmalate dehydrogenase [Cloning vector YIp351]
>gi|1546043|gb|AAB16841.1| (U64693) Leu2 [Cloning vector
pRSQ2-LEU2]
Length = 364
Score = 185 bits (466), Expect = 5e-46
Identities = 132/358 (36%), Positives = 199/358 (54%), Gaps = 24/358 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I V+PGD +G+E+ AE +KVLK + ++ S V F+F+ + G TG LPD A+E
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPDEALEA 65
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P+K
Sbjct: 66 SKKVDAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELQLYANLRPCNFASDSLLDLSPIKP 124
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKR 174
+ D V VRE G+Y G ++ +A T V+R+ R A F +
Sbjct: 125 QFAKGTDFVVVRELVGGIYFGKR---KEDDGDGVAWDSEQYTVPEVQRITRMAAFMALQH 181
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEYD-LETDHYYVDAMAMKMIRSPESFD- 231
+ +DKANVL + LW + E + E+ L+ H +D+ AM ++++P +
Sbjct: 182 EPPLPIWTLDKANVLASSR-LWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHLNG 240
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRIA 286
+++T NMFGDI++D G LG+ S ++ P+ G++EP HGSAP A ++
Sbjct: 241 IIITSNMFGDIISDEALRYPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPGFAKNKV- 299
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
+P+A ILSA+MML+ LNL +E K IE+AVK+ + T D+ G+ T EVGD V +
Sbjct: 300 DPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAE 357
>gb|AAF48965.1| (AE003512) CG12233 gene product [Drosophila melanogaster]
Length = 377
Score = 185 bits (466), Expect = 5e-46
Identities = 121/338 (35%), Positives = 189/338 (55%), Gaps = 23/338 (6%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
++ +IPGDGIG E+ A ++K+ + V E++ +P AI
Sbjct: 49 KVTLIPGDGIGPEISA----AVQKIFTAANVPIEWEAVDVTPVRGPDGKFGIPQAAI--- 101
Query: 64 KKFDAIYFGAIG--DPRVKP-GILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
D++ IG P + P G R + L +R E +LY N+RP + T +
Sbjct: 102 ---DSVNTNKIGLKGPLMTPVGKGHRSLNLALRKEFNLYANVRPCRSLEGYKTLY---DD 155
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+D+V +RENTEG Y+G + G Q I + T +RV +AF+YAK + RKKV
Sbjct: 156 VDVVTIRENTEGEYSGIEHEIVDGVVQSIK----LITEEASKRVAEYAFQYAKNNNRKKV 211
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T+V KAN++ + L+ R +++Q++ +++ + Y+D + + M+++P +DV+V PN++
Sbjct: 212 TVVHKANIMRMSDGLFLRCVRDMAQKFPEIQFEEKYLDTVCLNMVQNPGKYDVLVMPNLY 271
Query: 240 GDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
GDIL+D+ A +VGGLG+ SGN+ +FE VHG+APDIAGK +ANP A +LSA MML
Sbjct: 272 GDILSDMCAGLVGGLGLTPSGNMG-LNGALFESVHGTAPDIAGKDLANPTALLLSAVMML 330
Query: 300 EYLNLEKESKWIEEAVKRAIAENK-VTPDMNGNLKTYE 336
++ L + IE A I E K +T D+ G K E
Sbjct: 331 RHMELNTYADKIERAAFETIKEGKYLTGDLGGRAKCSE 368
>pir||T48350 3-isopropylmalate dehydrogenase-like protein - Arabidopsis thaliana
>gi|7378609|emb|CAB83285.1| (AL162751) 3-isopropylmalate
dehydrogenase-like protein [Arabidopsis thaliana]
>gi|9757780|dbj|BAB08389.1| (AB005240) 3-isopropylmalate
dehydrogenase [Arabidopsis thaliana]
Length = 372
Score = 185 bits (465), Expect = 6e-46
Identities = 117/332 (35%), Positives = 184/332 (55%), Gaps = 16/332 (4%)
Query: 7 VIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFKKF 66
+ PGDGIG E+ +KK+ + V E++E+ G E +T L ++E ++
Sbjct: 46 LFPGDGIGPEIAES----VKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRN 101
Query: 67 DAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIVFV 126
G + P K R + L +R EL+LY N+RP T + +D++ +
Sbjct: 102 KVGLKGPMATPIGKG---HRSLNLTLRKELNLYANVRPCYSLPGYKTRY---DDVDLITI 155
Query: 127 RENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVDKA 186
RENTEG Y+G + +G + + I TR RV +AF YAK GR++V+ + KA
Sbjct: 156 RENTEGEYSGLEHQVVRGVVESLK----IITRQASLRVAEYAFLYAKTHGRERVSAIHKA 211
Query: 187 NVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDILTD 245
N++ L+ + EV+++Y ++ + +D M ++++P FDV+V PN++GDI++D
Sbjct: 212 NIMQKTDGLFLKCCREVAEKYPEITYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISD 271
Query: 246 LGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLNLE 305
L A +VGGLG+ S NI + + E VHGSAPDIAGK +ANP A +LS MML +L
Sbjct: 272 LCAGLVGGLGLTPSCNIGEDGVALAEAVHGSAPDIAGKNLANPTALLLSGVMMLRHLKFN 331
Query: 306 KESKWIEEAVKRAIAENKV-TPDMNGNLKTYE 336
++++ I A+ IAE K T D+ G+ T E
Sbjct: 332 EQAEQIHSAIINTIAEGKYRTADLGGSSTTTE 363
>sp|P34733|LEU3_PICAN 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|629921|pir||S43454 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Pichia angusta)
>gi|392893|gb|AAA19109.1| (U00889) beta-isopropylmalate
dehydrogenase [Pichia angusta]
Length = 362
Score = 185 bits (464), Expect = 9e-46
Identities = 139/365 (38%), Positives = 200/365 (54%), Gaps = 33/365 (9%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M I ++PGD +G EVVAE +KVL+ + V F F ++ G G L D A+
Sbjct: 1 MSKNIVLLPGDHVGPEVVAEAVKVLEAVSSAIGVKFNFSKHLIGGASIDAYGVPLSDEAL 60
Query: 61 EEFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHP---RLTPL 115
E KK DA+ GA+G P+ G + E+G LLK+R EL+LY NLRP +L+PL
Sbjct: 61 EAAKKADAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELNLYANLRPCSFASDALLKLSPL 119
Query: 116 KGK--NKIDIVFVRENTEGLYAG-----AGGFLRKGTPQEIAVQEMINTRFGVERVIRFA 168
K + D V VRE G+Y G AG + T + +V E V+R+ R A
Sbjct: 120 KSEIVKGTDFVVVRELVGGIYFGDRKEDAGDGVASDT-ESYSVPE-------VQRITRMA 171
Query: 169 FEYAKRSGRK-KVTLVDKANVLTYAHDLWERVFAE-VSQEY-DLETDHYYVDAMAMKMIR 225
A +S + +DKANVL + LW + E + E+ L H +D+ AM +++
Sbjct: 172 AFLALQSDPPLPLWSLDKANVLASSR-LWRKTVEETIKNEFPQLTVQHQLIDSAAMILVK 230
Query: 226 SPESFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPD 279
SP + V+VT NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD
Sbjct: 231 SPTKLNGVIVTNNMFGDIISDEASVIPGSLGLLPSASLASLPDTNKAFGLYEPCHGSAPD 290
Query: 280 IAGKRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVG 338
+ ++ NPLA ILSA+MML+ L+L + IE+AVK + +T D+ G+ T EVG
Sbjct: 291 LGPGKV-NPLATILSAAMMLKLSLDLVDAGRAIEQAVKNVLDAGIMTADLGGSSSTQEVG 349
Query: 339 DWVVK 343
D V +
Sbjct: 350 DAVAQ 354
>sp|P18120|LEU3_YARLI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|173250|gb|AAA35244.1| (M37309)
beta-isopropylmalate dehydrogenase (EC 1.1.1.85)
[Yarrowia lipolytica]
>gi|7839536|gb|AAF70314.1|AF260230_1 (AF260230) Leu2
[Yarrowia lipolytica]
Length = 405
Score = 184 bits (463), Expect = 1e-45
Identities = 125/357 (35%), Positives = 194/357 (54%), Gaps = 26/357 (7%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
+I ++ GD G EV+AE +KVLK + E S F F++ G K GE + D ++
Sbjct: 14 KIVLLGGDFCGPEVIAEAVKVLKSVAEASGTEFVFEDRLIGGAAIEKEGEPITDATLDIC 73
Query: 64 KKFDAIYFGAIGD--------PRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRL--- 112
+K D+I GA+G P + + LLK+R +L+LY NLRP +L P+L
Sbjct: 74 RKADSIMLGAVGGAANTVWTTPDGRTDVRPEQGLLKLRKDLNLYANLRPCQLLSPKLADL 133
Query: 113 TPLKGKNKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEY 171
+P++ D + VRE G+Y G ++ +A + VER+ R A F
Sbjct: 134 SPIRNVEGTDFIIVRELVGGIYFGER---KEDDGSGVASDTETYSVPEVERIARMAAFLA 190
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY--DLETDHYYVDAMAMKMIRSPES 229
+ + V +DKANVL + LW + V ++ LE +H +D+ AM +I+ P
Sbjct: 191 LQHNPPLPVWSLDKANVLASSR-LWRKTVTRVLKDEFPQLELNHQLIDSAAMILIKQPSK 249
Query: 230 FD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGK 283
+ +++T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+ GK
Sbjct: 250 MNGIIITTNMFGDIISDEASVIPGSLGLLPSASLASLPDTNEAFGLYEPCHGSAPDL-GK 308
Query: 284 RIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGD 339
+ NP+A ILSA+MML++ LN++ +E AVK ++ T D+ G+ T EVGD
Sbjct: 309 QKVNPIATILSAAMMLKFSLNMKPAGDAVEAAVKESVEAGITTADIGGSSSTSEVGD 365
>emb|CAC10274.1| (AJ279020) putative beta-isopropylmalate dehydrogenase [Candida
rugosa]
Length = 359
Score = 184 bits (463), Expect = 1e-45
Identities = 142/365 (38%), Positives = 197/365 (53%), Gaps = 26/365 (7%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSR-VSFEFKEYPFGAEHYLKTGETLPDWA 59
M I ++PGD +G EVVAE +KVLK +E + SF F + G TG LPD A
Sbjct: 1 MSKSIVLLPGDHVGTEVVAEAVKVLKAIERTTPGTSFSFSTHLIGGAAIDATGVPLPDEA 60
Query: 60 IEEFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TP 114
+E K DA+ GA+G P+ G + E+G LLK+R EL LY NLRP +L +P
Sbjct: 61 LEAAKASDAVLLGAVGGPKWGTGDVRPEQG-LLKIRKELGLYANLRPCSFASSKLVDLSP 119
Query: 115 LKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEY 171
LK + D V VRE G+Y G ++ +A + VER+ R A F
Sbjct: 120 LKREIVEGTDFVVVRELVGGIYFGER---KEDDGSGVASDTETYSVPEVERITRMAAFLA 176
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY--DLETDHYYVDAMAMKMIRSPES 229
+ + + V +DKANVL + LW + V E + H +D+ AM +++ P
Sbjct: 177 LQHNPPQTVWSLDKANVLASSR-LWRKTVTRVMTEEFPTVPFQHQLIDSAAMILVQKPTK 235
Query: 230 FD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDI-AG 282
+ VV+T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+ AG
Sbjct: 236 LNGVVLTSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNTAFGLYEPCHGSAPDLPAG 295
Query: 283 KRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWV 341
K NPLA ILSA+MML L+ + IE+AV+ I T D+ G+ T EVGD +
Sbjct: 296 K--VNPLACILSAAMMLRLSLDNAAAADRIEQAVREVIDSGVATADLGGSSSTGEVGDAI 353
Query: 342 VKFLE 346
VK LE
Sbjct: 354 VKALE 358
>gb|AAC53643.1| (U38900) 3-isopropylmalate dehydrogenase [Cloning vector pClasper]
Length = 366
Score = 184 bits (462), Expect = 1e-45
Identities = 133/360 (36%), Positives = 202/360 (55%), Gaps = 26/360 (7%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPD--WAI 60
+I V+PGD +G+E+ AE +KVLK + ++ S V F+F+ + G TG LPD A+
Sbjct: 6 KIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHLIGGAAIDATGVPLPDEELAL 65
Query: 61 EEFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPL 115
E KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P+
Sbjct: 66 EASKKVDAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELQLYANLRPCNFASDSLLDLSPI 124
Query: 116 KGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYA 172
K + D V VRE G+Y G ++ +A T V+R+ R A F
Sbjct: 125 KPQFAKGTDFVVVRELVGGIYFGKR---KEDDGDGVAWDSEQYTVPEVQRITRMAAFMAL 181
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEYD-LETDHYYVDAMAMKMIRSPESF 230
+ + +DKANVL + LW + E + E+ L+ H +D+ AM ++++P
Sbjct: 182 QHEPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPTLKVQHQLIDSAAMILVKNPTHL 240
Query: 231 D-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKR 284
+ +++T NMFGDI++D + I G LG+ S ++ P+ G++EP HGSAPD+ +
Sbjct: 241 NGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKNK 300
Query: 285 IANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
+ +P+A ILSA+MML+ LNL +E K IE+AVK+ + T D+ G+ T EVGD V +
Sbjct: 301 V-DPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTGDLGGSNSTTEVGDAVAE 359
>sp|P87186|LEU3_CANAL 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2121286|gb|AAB58312.1| (AF000121)
isopropyl malate dehydrogenase [Candida albicans]
Length = 373
Score = 183 bits (461), Expect = 2e-45
Identities = 130/359 (36%), Positives = 196/359 (54%), Gaps = 23/359 (6%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEE---LSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
I V+PGD +G E+V E KVLK +E ++ F+FK + G TG LPD A+E
Sbjct: 8 ITVLPGDHVGTEIVNEPTKVLKAIEAPTPYQKIQFDFKHHLIGGAAIDATGVPLPDDALE 67
Query: 62 EFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHP---RLTPLK 116
K DA+ GA+G P+ G + E+G LLK+R EL+LY N+RP L+PLK
Sbjct: 68 SAKSSDAVLLGAVGGPKWGTGTVRPEQG-LLKIRKELNLYANIRPCNFASDSLLELSPLK 126
Query: 117 GK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAK 173
+ +++ VRE G+Y G + + A T V R+ R A F +
Sbjct: 127 AEVVKGTNLIIVRELVGGIYFGERQEQEESEDHQTAWYRPKYTVDEVTRITRMAAFMALQ 186
Query: 174 RSGRKKVTLVDKANVLTYAHDLWERVFAEV-SQEYD-LETDHYYVDAMAMKMIRSPESFD 231
+ + +DKANVL + LW + +V S+E+ L H +D+ AM +I++P
Sbjct: 187 HNPPLPIWSLDKANVLASSR-LWRKTVDKVISEEFPALSVQHQLIDSAAMILIQNPTKLS 245
Query: 232 -VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRI 285
+++T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+ ++
Sbjct: 246 GIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDTNTAFGLYEPCHGSAPDLPANKV 305
Query: 286 ANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
NP+A ILSA+ ML L+ KE++ +EEAVK+ + T D+ G T EVGD + +
Sbjct: 306 -NPIATILSAASMLRLALDCVKETEALEEAVKQVLDSGIRTADLRGTSSTTEVGDAIAE 363
>pir||T03406 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) precursor -
common tobacco >gi|3021506|emb|CAA65502.1| (X96727)
isocitrate dehydrogenase (NAD+) [Nicotiana tabacum]
Length = 364
Score = 183 bits (461), Expect = 2e-45
Identities = 116/334 (34%), Positives = 183/334 (54%), Gaps = 16/334 (4%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+R + PGDGIG E+ ++ K E V E++E+ G E +T L ++E
Sbjct: 34 IRATLFPGDGIGPEIADSVRQIFKTAE----VPIEWEEHYVGKEIDPRTNSFLTWESLES 89
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
++ G + P K R + L +R EL+LY N+RP T + ++
Sbjct: 90 VRRNKVGLKGPMATPIGKG---HRSLNLTLRKELNLYANVRPCYSLPGYKTRY---DDVN 143
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
++ +RENTEG Y+G + +G + + I TR RV +AF YAK GR++V+
Sbjct: 144 LITIRENTEGEYSGVEHQVVRGVVESLK----IITRQASLRVAEYAFHYAKAHGRERVSA 199
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
+ KAN++ L+ + EV+++Y +++ + +D M ++++P FD++V PN++GD
Sbjct: 200 IHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPALFDILVMPNLYGD 259
Query: 242 ILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEY 301
I++DL A ++GGLG+ S NI + E VHGSAPDIAGK +ANP A +LSA ML +
Sbjct: 260 IISDLCAGVIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSAVTMLRH 319
Query: 302 LNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKT 334
L L ++ I+ AV IAE K T D+ G T
Sbjct: 320 LELHDKADRIQGAVLNTIAEGKYRTGDLGGTSST 353
>prf||2004294A beta isopropylmalate dehydrogenase [Neurospora crassa]
Length = 368
Score = 182 bits (458), Expect = 4e-45
Identities = 136/368 (36%), Positives = 196/368 (52%), Gaps = 32/368 (8%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLE--ELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
I V G+ G EVV E +KVLK +E S F + + G K + L D A+
Sbjct: 6 IVVFGGNHCGPEVVLEAIKVLKAIETNSPSACKFNLQNHLLGGASIDKHNDPLTDEALNA 65
Query: 63 FKKFDAIYFGAIGDPR------VKPGILERGILLKMRFELDLYVNLRPVKLYHPRL---T 113
K DA+ GAIG P V+P E+G LLK+R EL Y NLRP L +
Sbjct: 66 AKAADAVLLGAIGGPEWGTSSTVRP---EQG-LLKLRKELGTYGNLRPCNFASESLVDSS 121
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FE 170
PLK + D + VRE T G+Y G + A +R + R+ R A F
Sbjct: 122 PLKAEVCRGTDFIVVRELTGGIYFGDR---TEDDGSGYACDTEPYSRAEIVRIARLAGFL 178
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEV-SQEY-DLETDHYYVDAMAMKMIRSPE 228
++ KV +DKANVL + LW + +V S+E+ L+ +H +D+ AM ++++P
Sbjct: 179 ALAKNPPAKVWSLDKANVLATSR-LWRKTVTDVISKEFPQLQLEHQLIDSAAMLLVKNPR 237
Query: 229 SFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNINP------ETTGMFEPVHGSAPDIA 281
+ + VV+T N+FGDI++D + I G +G+ S ++ + G++EP+HGSAPDI+
Sbjct: 238 ALNGVVITSNLFGDIISDEASVIPGSIGLLPSASLGGIPDGKGKCNGIYEPIHGSAPDIS 297
Query: 282 GKRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDW 340
GK I NP+ ILS +MML Y LNL KE+ +E AVK AI T D+ GN T ++G+
Sbjct: 298 GKGIVNPVGTILSVAMMLRYSLNLPKEADAVEAAVKAAIDNGTKTKDLGGNATTSDMGNA 357
Query: 341 VVKFLEVV 348
VV LE +
Sbjct: 358 VVAELEKI 365
>sp|P29696|LEU3_SOLTU 3-ISOPROPYLMALATE DEHYDROGENASE PRECURSOR (BETA-IPM DEHYDROGENASE)
(IMDH) (3-IPM-DH) >gi|82259|pir||S25670
3-isopropylmalate dehydrogenase (EC 1.1.1.85) precursor
- potato >gi|22643|emb|CAA47720.1| (X67310)
3-isopropylmalate dehydrogenase [Solanum tuberosum]
>gi|445064|prf||1908380A beta isopropylmalate
dehydrogenase [Solanum tuberosum]
Length = 357
Score = 182 bits (456), Expect = 7e-45
Identities = 113/323 (34%), Positives = 184/323 (55%), Gaps = 15/323 (4%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+R + PGDGIG E+ AE ++ + K+ E V E++E+ G E +T L ++E
Sbjct: 37 IRATLFPGDGIGPEI-AESVRQIFKVAE---VPIEWEEHYVGTEVDPRTNSFLTWESLES 92
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
++ G + P K R + L +R EL+LY N+RP T + ++
Sbjct: 93 VRRNKVGLKGPMATPIGKG---HRSLNLTLRKELNLYANVRPCYSLPGYKTRY---DDVN 146
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
++ +RENTEG Y+G + +G + + I TR RV +AF YAK GR++V+
Sbjct: 147 LITIRENTEGEYSGLEHQVVRGVVESLK----IITRQASLRVAEYAFHYAKTHGRERVSA 202
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
+ KAN++ L+ + EV+++Y +++ + +D M ++++P FDV+V PN++GD
Sbjct: 203 IHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGD 262
Query: 242 ILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEY 301
I++DL A ++GGLG+ S NI + E VHGSAPDIAGK +ANP A +LS+ ML +
Sbjct: 263 IISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVSMLRH 322
Query: 302 LNLEKESKWIEEAVKRAIAENKV 324
L L ++ I++A+ + IA KV
Sbjct: 323 LELHDKADRIQDAILKTIAGGKV 345
>sp|P34738|LEU3_NEUCR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|11251549|pir||T46607 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) beta [imported] - Neurospora
crassa >gi|4204106|gb|AAD10616.1| (U01061)
beta-isopropylmalate dehydrogenase [Neurospora crassa]
Length = 368
Score = 182 bits (456), Expect = 7e-45
Identities = 137/368 (37%), Positives = 193/368 (52%), Gaps = 32/368 (8%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLE--ELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
I V GD G EVV E +KVLK +E S F + + G K + L D A+
Sbjct: 6 IVVFGGDHCGPEVVLEAIKVLKAIETNSPSACKFNLQNHLLGGASIDKHNDPLTDEALNA 65
Query: 63 FKKFDAIYFGAIGDPR------VKPGILERGILLKMRFELDLYVNLRPVKLYHPRL---T 113
K DA+ GAIG P V+P E+G LLK+R EL Y NLRP L +
Sbjct: 66 AKAADAVLLGAIGGPEWGTSSTVRP---EQG-LLKLRKELGTYGNLRPCNFASESLVDSS 121
Query: 114 PLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FE 170
PLK + D + VRE T G+Y G + A +R + R+ R A F
Sbjct: 122 PLKAEVCRGTDFIVVRELTGGIYFGDR---TEDDGSGYACDTEPYSRAEIVRIARLAGFL 178
Query: 171 YAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY--DLETDHYYVDAMAMKMIRSPE 228
++ KV +DKANVL + LW + +V + L+ +H +D+ AM ++++P
Sbjct: 179 ALAKNSPAKVWSLDKANVLATSR-LWRKTVTDVISKECPQLQLEHQLIDSAAMLLVKNPR 237
Query: 229 SFD-VVVTPNMFGDILTDLGAEIVGGLGIAAS---GNI---NPETTGMFEPVHGSAPDIA 281
+ + VV+T N+FGDI++D + I G +G+ S G I + G++EP+HGSAPDI+
Sbjct: 238 ALNGVVITSNLFGDIISDEASVIPGSIGLLPSASWGGIPDGKVKCNGIYEPIHGSAPDIS 297
Query: 282 GKRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDW 340
GK I NP+ ILS +MML Y LNL KE+ +E AVK AI T D+ GN T ++G+
Sbjct: 298 GKGIVNPVCTILSVAMMLRYSLNLPKEADAVEAAVKAAIDNGTKTKDLGGNATTSDMGNA 357
Query: 341 VVKFLEVV 348
VV LE +
Sbjct: 358 VVAELEKI 365
>dbj|BAA88179.1| (AP000836) ESTs C19133(E10008),AU058199(E50136), AU081337(E50136)
correspond to a region of the predicted gene.~Similar to
isocitrate dehydrogenase (X96727) [Oryza sativa]
Length = 362
Score = 181 bits (455), Expect = 1e-44
Identities = 115/334 (34%), Positives = 184/334 (54%), Gaps = 16/334 (4%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+R + PGDGIG E+ +K++ ++ V E++E+ G E +T L ++E
Sbjct: 32 VRATLFPGDGIGPEIAES----VKQVFNVAGVPIEWEEHYVGTEVDPRTESFLTWESLES 87
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
++ G + P K R + L +R EL LY N+RP T + ++
Sbjct: 88 VRRNKVGLKGPMATPIGKG---HRSLNLTLRKELGLYANVRPCNSLPGYKTRY---DDVN 141
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
+V +RENTEG Y+G + +G + + I TR RV +AF YAK +GR++V+
Sbjct: 142 LVTIRENTEGEYSGLEHQVVRGVVESLK----IITRQASLRVAEYAFHYAKTNGRERVSA 197
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
+ KAN++ L+ + EV+++Y ++ + +D M ++++P FDV+V PN++GD
Sbjct: 198 IHKANIMRKTDGLFLKCCREVAEKYPEIVYEEVIIDNCCMTLVKNPGLFDVLVMPNLYGD 257
Query: 242 ILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEY 301
I++DL A ++GGLG+ S NI + E VHGSAPDIAGK +ANP A +LSA MML +
Sbjct: 258 IISDLCAGLIGGLGLTPSCNIGEGGICLAEAVHGSAPDIAGKNLANPTALMLSAVMMLRH 317
Query: 302 LNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKT 334
L ++ I A+ + I+E K T D+ G T
Sbjct: 318 LQFNNQADRIHNAILQTISEGKFRTADLGGKAST 351
>pir||S30897 3-isopropylmalate dehydrogenase (EC 1.1.1.85) precursor - potato
Length = 357
Score = 181 bits (455), Expect = 1e-44
Identities = 113/323 (34%), Positives = 184/323 (55%), Gaps = 15/323 (4%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+R + PGDGIG E+ AE ++ + K+ E V E++E+ G E +T L ++E
Sbjct: 37 IRATLFPGDGIGPEI-AESVRQIFKVAE---VPIEWEEHYVGTEVDPRTNSFLTWESLES 92
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
++ G + P K R + L +R EL+LY N+RP T + ++
Sbjct: 93 VRRNKVGLKGPMATPIGKG---HRSLNLTLRKELNLYANVRPCYSLPGYKTRY---DDVN 146
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
++ +RENTEG Y+G + +G + + I TR RV +AF YAK GR++V+
Sbjct: 147 LITIRENTEGEYSGLEHQVVRGVVESLK----IITRQASLRVAEYAFHYAKTHGRERVSA 202
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
+ KAN++ L+ + EV+++Y +++ + +D M ++++P FDV+V PN++GD
Sbjct: 203 IHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCMMMLVKNPALFDVLVMPNLYGD 262
Query: 242 ILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEY 301
I++DL A ++GGLG+ S NI + E VHGSAPDIAGK +ANP A +LS+ ML +
Sbjct: 263 IISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVSMLRH 322
Query: 302 LNLEKESKWIEEAVKRAIAENKV 324
L L ++ I++A+ + IA KV
Sbjct: 323 LELHDKADRIQDAILKTIAGGKV 345
>pir||T50386 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) chain 2
precursor, mitochondrial [imported] - fission yeast
(Schizosaccharomyces pombe) >gi|6522995|emb|CAB62099.1|
(AL133306) probable isocitrate dehydrogenase [nad]
subunit 2, mitochondrial precursor(ec 1.1.1.41)
[Schizosaccharomyces pombe]
Length = 378
Score = 180 bits (453), Expect = 2e-44
Identities = 123/351 (35%), Positives = 192/351 (54%), Gaps = 29/351 (8%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLE---ELSRVSFEFKEYPFGAEHYLKTGET-LPDWAI 60
+ +I GDGIG E+ ++ K + E RV K YP LK G T +PD A
Sbjct: 48 VTMIAGDGIGPEIAQSVERIFKAAKVPIEWERV----KVYPI-----LKNGTTTIPDDAK 98
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
E +K G + P + G + + L+ F L+ N+RP TP +
Sbjct: 99 ESVRKNKVALKGPLATP-IGKGHVSMNLTLRRTF--GLFANVRPCVSITGYKTPY---DN 152
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
++ V +RENTEG Y+G + G Q I + TR ERVIR+AF+YA+++G+ +
Sbjct: 153 VNTVLIRENTEGEYSGIEHEVIPGVVQSIK----LITRAASERVIRYAFQYARQTGKNNI 208
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD--VVVTPN 237
T+V KA ++ A L+ E++ EY D+E +D +K++ P ++ V+V PN
Sbjct: 209 TVVHKATIMRMADGLFLECAKELAPEYPDIELREEILDNACLKIVTDPVPYNNTVMVMPN 268
Query: 238 MFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASM 297
++GDI++D+ A ++GGLG+ SGNI + + +FE VHG+APDIAGK +ANP A +LS+ M
Sbjct: 269 LYGDIVSDMCAGLIGGLGLTPSGNIGNQAS-IFEAVHGTAPDIAGKGLANPTALLLSSVM 327
Query: 298 MLEYLNLEKESKWIEEAVKRAIAEN--KVTPDMNGNLKTYEVGDWVVKFLE 346
ML+++NL +K IE A+ +A N T D+ G + D ++ L+
Sbjct: 328 MLKHMNLNDYAKRIESAIFDTLANNPDARTKDLGGKSNNVQYTDAIISKLK 378
>sp|O60027|LEU3_ASHGO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|3171729|emb|CAA07006.1| (AJ006406)
beta-isopropylmalatedehydrogenase [Eremothecium
gossypii] >gi|3873558|emb|CAA04541.1| (AJ001115)
b-isopropylmalate dehydrogenase [Eremothecium gossypii]
Length = 372
Score = 180 bits (452), Expect = 2e-44
Identities = 135/355 (38%), Positives = 197/355 (55%), Gaps = 26/355 (7%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEE-LSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
RI V+PGD IG+EVV E +KVL +E+ LS V F+F+ + G TG LPD A+
Sbjct: 6 RIVVLPGDHIGREVVEEAVKVLGAVEQSLSDVHFDFQYHLVGGAAIDATGSALPDEALGA 65
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHP---RLTPLKG 117
K+ DA+ GA+G P+ + G + E+G LLK+R EL +Y NLRP L+PL+
Sbjct: 66 AKEADAVLLGAVGGPKWQGGAVRPEQG-LLKLRQELGVYANLRPCNFAADSLLELSPLRP 124
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKR 174
+ DI+ VRE G Y G + +A T V+R+ R A F +
Sbjct: 125 EIARDTDIMVVRELLGGSYFGER---HEDEGDGVAWDTDKYTVKEVQRIARMAGFLALQH 181
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY--DLETDHYYVDAMAMKMIRSPESFD- 231
V +DKANVL + LW + E Q +++ H +D+ AM ++++P +F+
Sbjct: 182 DPPLPVWSLDKANVLASSR-LWRKTVEETFQSEFPNVQLQHQLIDSAAMILVKNPRAFNG 240
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNI-----NPETTGMFEPVHGSAPDI-AGKRI 285
VVVT NMFGDI++D + I G LG+ S ++ + G++EP HGSAPD+ AGK
Sbjct: 241 VVVTSNMFGDIISDEASVIPGSLGLLPSASLASLPDSKSAFGLYEPCHGSAPDLPAGK-- 298
Query: 286 ANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGD 339
ANP+ ILSA+MML+ LN+ + +E+AV+ + T D+ G+ T EVGD
Sbjct: 299 ANPIGCILSAAMMLKLSLNMVAAGEAVEQAVQEVLDSGVRTGDLLGSSSTSEVGD 353
>sp|P24404|LEU3_AGRTU 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|142237|gb|AAA22089.1| (M38670)
beta-isopropylmalate dehydrogenase [Agrobacterium
tumefaciens]
Length = 370
Score = 180 bits (451), Expect = 3e-44
Identities = 123/360 (34%), Positives = 189/360 (52%), Gaps = 25/360 (6%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDGIG E + E K+++ + F E G Y G + D +E+
Sbjct: 6 VFLLPGDGIGPEAMTEVRKLIEYMNSAHNAGFTVSEGLVGGSAYDAHGVAISDADMEKAL 65
Query: 65 KFDAIYFGAIGDPRVKPGILER---GILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK- 120
DAI FGA+G P+ E LL++R +L+L+ NLRP Y P L
Sbjct: 66 AADAILFGAVGGPKWDGVPYEHRPEAGLLRLRKDLELFANLRPAICY-PALAAASSLKPE 124
Query: 121 ----IDIVFVRENTEGLYAGAGG-FLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRS 175
+DI+ VRE T G+Y G + G Q+ + I F +ER+ AFE A RS
Sbjct: 125 LVEGLDILIVRELTGGVYFGEPKQIIDLGNGQKRGIDTQIYDTFEIERIASVAFELA-RS 183
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAEV--SQEYDLETDHYYVDAMAMKMIRSPESFDVV 233
+V ++K NV+ + LW +V E ++ D++ +H DA M+++R P+ FDV+
Sbjct: 184 RDNRVCSMEKRNVMK-SGVLWNQVVTETHAAKYKDVQLEHMLADAGGMQLVRKPKQFDVI 242
Query: 234 VTPNMFGDILTDLGAEIVGGLGIAASGNINP------ETTGMFEPVHGSAPDIAGKRIAN 287
VT N+FGD+L+D+ A + G LG+ S ++ + M+EPVHGSAPDIAGK IAN
Sbjct: 243 VTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDAKTGKRKAMYEPVHGSAPDIAGKSIAN 302
Query: 288 PLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGN----LKTYEVGDWVV 342
P+A I S +M L Y N+ E+ +E A+ + + T D+ + + T ++GD V+
Sbjct: 303 PIAMIASFAMCLRYSFNMVDEATKLEAAIANVLDKGIRTADIMADGCRQVGTSDMGDAVL 362
>pir||G75355 3-isopropylmalate dehydrogenase - Deinococcus radiodurans (strain
R1) >gi|6459553|gb|AAF11333.1|AE002019_6 (AE002019)
3-isopropylmalate dehydrogenase [Deinococcus
radiodurans]
Length = 352
Score = 180 bits (451), Expect = 3e-44
Identities = 128/355 (36%), Positives = 193/355 (54%), Gaps = 25/355 (7%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
++ +PGDGIG EV A ++VL+++ V +E+ G Y GE P +
Sbjct: 3 KVITLPGDGIGPEVTAAAVQVLREVAPDVTV----EEHAIGGGAYDAHGEPFPTSTRDAL 58
Query: 64 KKFDAIYFGAIGDP------RVKPGILERGILLKMRFELDLYVNLRPVKLYH--PRLTPL 115
K+ DA+ G +G ++ + LL +R L Y NLRPV++ L+PL
Sbjct: 59 KEADAVLLGTVGGAHDSAWNQLPRHLRPESGLLALRKALGCYANLRPVRVQPGLEHLSPL 118
Query: 116 KGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAK 173
K + +DI+ VRE G+Y + T A M T VERV + AF +A
Sbjct: 119 KAELARGVDILIVRELLGGIYFDQDRKIEGDT----AYNTMRYTTSEVERVAKVAF-WAA 173
Query: 174 RSGRKKVTLVDKANVLTYAHDLWER-VFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
+ +VT VDKANVL + +LW R V A ++Y + +H YVD++AM ++ P +D
Sbjct: 174 EQRKGRVTSVDKANVLEVS-ELWRRDVQALRDRDYRSIHLNHEYVDSVAMLIVADPSRYD 232
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAA 291
V++T N+FGDIL+DL A I G LG+ S ++ + G+FEP+HGSAPDIAG+ +ANP AA
Sbjct: 233 VILTENLFGDILSDLAAVIPGSLGLMPSASLG-DGAGLFEPIHGSAPDIAGQGVANPAAA 291
Query: 292 ILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
I+S M+L + L + + ++ AV A+ E + T D+ G+ T V+K L
Sbjct: 292 IMSVGMLLRHGLERSEAANQVDRAVALALRE-QPTRDLGGSADTQTFTRAVLKAL 345
>gb|AAF23254.1|AC015985_12 (AC015985) putative isocitrate dehydrogenase (NAD+) [Arabidopsis
thaliana]
Length = 374
Score = 179 bits (450), Expect = 4e-44
Identities = 118/334 (35%), Positives = 180/334 (53%), Gaps = 16/334 (4%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
++ + PGDGIG E+ +K++ + V ++ E G E +T L ++
Sbjct: 44 IKATLFPGDGIGPEIAES----VKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQS 99
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
K G + P K R + L +R EL+LY N+RP T + +D
Sbjct: 100 VLKNKVGLKGPMATPIGKG---HRSLNLTLRKELNLYANVRPCYSLPGYKTRY---DDVD 153
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
++ +RENTEG Y+G + KG + + I TR RV +AF YAK GRKKV+
Sbjct: 154 LITIRENTEGEYSGLEHQVVKGVVESLK----IITRKASMRVAEYAFLYAKTHGRKKVSA 209
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
+ KAN++ L+ + EV+ +Y ++ + +D M ++++P FDV+V PN++GD
Sbjct: 210 IHKANIMQKTDGLFLQCCDEVAAKYPEIYYEKVVIDNCCMMLVKNPALFDVLVMPNLYGD 269
Query: 242 ILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEY 301
I++DL A +VGGLG+ S NI + + E VHGSAPDIAG +ANP A +LS MML +
Sbjct: 270 IISDLCAGLVGGLGLTPSMNIGEDGIALAEAVHGSAPDIAGMNLANPTALLLSGVMMLRH 329
Query: 302 LNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKT 334
L L K+++ I A+ IAE K T D+ G+ T
Sbjct: 330 LKLNKQAEQIHSAIINTIAEGKYRTADLGGSSTT 363
>sp|P41766|LEU3_KLUMA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|479255|pir||S32969 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) - yeast (Kluyveromyces
marxianus) >gi|2700|emb|CAA43710.1| (X61490)
3-isopropylmalate dehydrogenase [Kluyveromyces
marxianus]
Length = 359
Score = 177 bits (445), Expect = 1e-43
Identities = 132/366 (36%), Positives = 198/366 (54%), Gaps = 27/366 (7%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELS-RVSFEFKEYPFGAEHYLKTGETLPDWA 59
M I V+PGD +G E+ E +KVL + E + F F+ + G TG LPD
Sbjct: 1 MSKNIVVLPGDHVGTEITNEAIKVLNAISEARPSIKFNFEHHLIGGAAIDATGVPLPD-- 58
Query: 60 IEEFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TP 114
E KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +P
Sbjct: 59 -EASKKADAVLLGAVGGPKWGTGSVRPEQG-LLKIRKELGLYANLRPCNFASDSLLDLSP 116
Query: 115 LKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEY 171
LK + D V VRE G+Y G ++ +A + V+R+ R A F
Sbjct: 117 LKPEYAKGTDFVVVRELVGGIYFGER---KEDEGDGVAWDSEKYSVPEVQRITRMAAFLA 173
Query: 172 AKRSGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEY-DLETDHYYVDAMAMKMIRSPES 229
+ + + +DKANVL + LW + E + E+ L H +D+ AM +++SP
Sbjct: 174 LQHNPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPQLTVQHQLIDSAAMILVKSPTK 232
Query: 230 FD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGK 283
+ +V+T NMFGDI++D + I G LG+ S ++ P+T G++EP HGSAPD+
Sbjct: 233 LNGIVITNNMFGDIISDEASVIPGSLGLLPSASLASLPDTNKAFGLYEPCHGSAPDLPAN 292
Query: 284 RIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVV 342
++ NP+A ILSA+MML+ L+L +E + +EEAV++ + T D+ G+ T EVGD V
Sbjct: 293 KV-NPIATILSAAMMLKLSLDLVEEGRAVEEAVRKVLDSGIRTGDLGGSNSTTEVGDAVA 351
Query: 343 KFLEVV 348
K ++ +
Sbjct: 352 KAVKEI 357
>sp|O14429|LEU3_CANGA 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2231676|gb|AAB62089.1| (U90626) LEU2
[Candida glabrata]
Length = 365
Score = 176 bits (443), Expect = 2e-43
Identities = 131/358 (36%), Positives = 196/358 (54%), Gaps = 25/358 (6%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEEL--SRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
I V PGD +G+E+ E KVL ++E +V+F+F + G TG LPD A+E
Sbjct: 7 IVVPPGDHVGQEITEEANKVLNAIQECRPDKVNFKFDHHLIGGAPIDATGVPLPDEALEA 66
Query: 63 FKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
KK DA+ GA+G P+ G + E+G LLK+R EL LY NLRP L +PLK
Sbjct: 67 SKKADAVLLGAVGGPKWGTGAVRPEQG-LLKIRKELQLYANLRPCNFASDSLLDLSPLKP 125
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKR 174
+ D V VRE G+Y G ++ +A + V+R+ R A F +
Sbjct: 126 EIARGTDFVVVRELVGGIYFGER---KEDEGDGVAWDSEKYSVPEVQRITRMAAFMALQH 182
Query: 175 SGRKKVTLVDKANVLTYAHDLWERVFAE-VSQEY-DLETDHYYVDAMAMKMIRSPESFD- 231
+ + +DKANVL + LW + E + E+ L H +D+ AM ++++P +
Sbjct: 183 NPPLPIWSLDKANVLASSR-LWRKTVEETIKNEFPQLTVQHQLIDSAAMILVKNPTHLNG 241
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRIA 286
+++T NMFGDI++D + I G LG+ S ++ P+ G++EP HGSAPD+ ++
Sbjct: 242 IIITNNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGSAPDLPKGKV- 300
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVK 343
NP+A ILSA+MML+ L+L +E + IE+AVK+ + T D+ G T EVGD V K
Sbjct: 301 NPVATILSAAMMLKLSLDLFEEGEIIEQAVKKVLDSGIRTADLKGTNSTTEVGDAVAK 358
>sp|Q12545|LEU3_CEPAC 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|840666|dbj|BAA09319.1| (D50665)
beta-isopropylmalate dehydrogenase [Acremonium
chrysogenum]
Length = 380
Score = 176 bits (441), Expect = 4e-43
Identities = 132/374 (35%), Positives = 193/374 (51%), Gaps = 40/374 (10%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLE-ELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I V+PGD IG E++AE +KVL +E + F G G + ++
Sbjct: 6 KILVLPGDHIGPEIMAEAIKVLTTIETHRPNLHFNLTTDLVGGTSIDTHGVPITQSVLDA 65
Query: 63 FKKFDAIYFGAIGDPR---VKPGILERGILLKMRFELDLYVNLRPVKLYHPRL---TPLK 116
K DA+ FG+IG P V P E G LL++R LD + NLRP + P L +P++
Sbjct: 66 AKASDAVLFGSIGGPEWAGVHP-TPESG-LLQLRQHLDAFANLRPCEFLVPSLVGASPIR 123
Query: 117 GK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKR 174
+ VREN G Y G K +++A + TR +ER+ R + A+
Sbjct: 124 EHVVKGTRFIVVRENCGGAYFG-----EKKEEEDVASDLWVYTRPEIERLARVSAAVARI 178
Query: 175 SGRKK-------VTLVDKANVLTYAHDLWERVFAEV-SQEY-DLETDHYYVDAMAMKMIR 225
GR + V DKANVL LW R+ +++ ++E+ D+ H D+MAM M+R
Sbjct: 179 MGRSEDDNQAATVWSADKANVLASGR-LWRRITSDIFAKEFPDITLQHQLADSMAMLMVR 237
Query: 226 SPESFDVVV-TPNMFGDILTDLGAEIVGGLGIAASGNI------NPETTGMFEPVHGSAP 278
P F+ V+ T N FGDIL+D+ I G LG+ S ++ + G++EPVHGSAP
Sbjct: 238 DPRRFNGVIHTDNTFGDILSDISGAITGTLGLMPSASLCGVPGEGHRSNGIYEPVHGSAP 297
Query: 279 DIAGKRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKV------TPDMNGN 331
DI+GK +ANP+A ILS +MML Y + LEKE+ +E AV + + T D+ G
Sbjct: 298 DISGKGLANPVAQILSVAMMLRYSMGLEKEATAVERAVVKVLDAKSEGGLEIRTGDLGGR 357
Query: 332 LKTYEVGDWVVKFL 345
+VGD V + L
Sbjct: 358 ATCSQVGDAVCEVL 371
>sp|P87256|LE3A_ASPNG 3-ISOPROPYLMALATE DEHYDROGENASE A (BETA-IPM DEHYDROGENASE A) (IMDH
A) (3-IPM-DH A) >gi|7431319|pir||S72209
3-isopropylmalate dehydrogenase (EC 1.1.1.85) A -
Aspergillus niger >gi|1911741|gb|AAB50827.1| (S83228)
beta-isopropylmalate dehydrogenase [Aspergillus niger,
strain A733, Peptide, 363 aa] >gi|4097255|gb|AAD10278.1|
(U51130) beta-isopropylmalate dehydrogenase [Aspergillus
niger]
Length = 363
Score = 176 bits (441), Expect = 4e-43
Identities = 126/360 (35%), Positives = 190/360 (52%), Gaps = 24/360 (6%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEEL-SRVSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
I V GD G EV AE +KVL+ +E+ ++ +++ G T L D A+
Sbjct: 6 IVVFAGDHWGPEVTAEAIKVLRVIEKSRDDITLNLQDHLLGGASIDATANPLTDEALAAA 65
Query: 64 KKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKGK 118
K DA+ GAIG P+ G + E+G LL +R E+ + NLRP P L +PLK
Sbjct: 66 KNADAVLLGAIGGPKWGTGAVRPEQG-LLNVRKEMGTFGNLRPCNFAAPSLVEHSPLKAS 124
Query: 119 --NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSG 176
+D +RE T G+Y G RK + +R +ER+ A + +
Sbjct: 125 VCEGVDFNIIRELTGGIYFGD----RKKMTAAATMDTEPYSRAEIERITPRAHLALQHNP 180
Query: 177 RKKVTLVDKANVLTYAHDLWERVFAEV-SQEY-DLETDHYYVDAMAMKMIRSPESFD-VV 233
V +DKANVL + LW + E+ ++E+ L+ +H +D+ AM M+++P + ++
Sbjct: 181 PLPVWSLDKANVLATSR-LWRKTVTEIMAKEFPQLKIEHQLIDSAAMIMVKNPRQLNGII 239
Query: 234 VTPNMFGDILTDLGAEIVGGLGIAASGNINP------ETTGMFEPVHGSAPDIAGKRIAN 287
VT N+FGDI++D + I G LG+ S +++ G++EP+HGSAPDIAGK I N
Sbjct: 240 VTSNLFGDIISDEASVIPGSLGLLPSASLSGIPDGKGRVNGIYEPIHGSAPDIAGKGIVN 299
Query: 288 PLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFLE 346
P+AAILS +MM++Y E++ IE AV+ + T D+ G T EVGD V LE
Sbjct: 300 PVAAILSVAMMMQYSFGRFDEARAIEAAVRNVLESGVRTGDIGGKATTSEVGDAVAAELE 359
>sp|Q9Y897|LEU3_MYCGR 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|5107397|gb|AAD40111.1|AF156181_1
(AF156181) 3-isopropylmalate dehydrogenase
[Mycosphaerella graminicola]
Length = 365
Score = 175 bits (439), Expect = 7e-43
Identities = 133/363 (36%), Positives = 194/363 (52%), Gaps = 24/363 (6%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSR-VSFEFKEYPFGAEHYLKTGETLPDWAIEEF 63
I V GD G EV AE LKVL ++ + V F + + G GE L D A+
Sbjct: 6 IVVFGGDHCGPEVTAEALKVLDVIDNSNADVHFNIQPHLLGGASIDAHGEPLTDEALAAA 65
Query: 64 KKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKGK 118
K DA+ GAIG P+ G + E+GIL ++R E+ Y NLRP L +PLK +
Sbjct: 66 KAADAVILGAIGGPKWGTGKVRPEQGIL-RLRKEMGTYGNLRPCFFASESLVKTSPLKEE 124
Query: 119 --NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAKRS 175
++ VRE T G+Y G + AV +R +ERV R A F
Sbjct: 125 VCRGVNFNIVRELTGGIYFGER---TEDDGSGYAVDTEPYSRAEIERVARLAGFLALAED 181
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAEV-SQEY-DLETDHYYVDAMAMKMIRSPESFD-V 232
V +DKANV+ + LW + +V + E+ L+ H+ +D+ AM M+++P + + V
Sbjct: 182 PPCPVWSLDKANVMATSR-LWRKTVTDVFANEFPQLKIGHHLIDSAAMLMVKNPRALNGV 240
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNINP------ETTGMFEPVHGSAPDIAGKRIA 286
+VT N+FGDI++D + I G LG+ S ++ + G++EP+HGSAPDI+GK I
Sbjct: 241 IVTSNLFGDIISDEASVIPGSLGLLPSASLTASPDGKSKCNGIYEPIHGSAPDISGKGIV 300
Query: 287 NPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVVKFL 345
NP+A ILS MM +Y L + +K I+EAV+ I + T D+ G+ KT EVGD + K L
Sbjct: 301 NPVAMILSLGMMCKYSLQQPELAKKIDEAVRNVIEKGINTADIGGSAKTAEVGDAIAKEL 360
Query: 346 EVV 348
E +
Sbjct: 361 EAL 363
>sp|Q48806|DLPA_LEGPN DLPA PROTEIN >gi|2120838|pir||S61390 dlpA protein - Legionella
pneumophila >gi|563806|gb|AAA79904.1| (U07940) DlpA
[Legionella pneumophila]
Length = 615
Score = 175 bits (438), Expect = 1e-42
Identities = 124/370 (33%), Positives = 185/370 (49%), Gaps = 41/370 (11%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
++IAV+PGDGIG EV L V + L+ V G E + K G +P +
Sbjct: 7 IKIAVLPGDGIGIEVTEATLPVFEVLD----VPVILNYGDIGWEFWKKEGAAIPSRTWQL 62
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLK------------MRFELDLYVNLRPVKLYHP 110
D + GAI + E LK +R LDL+ N+RP
Sbjct: 63 IASSDTVLLGAITSKPQREAKQELSNALKKSNPYYVSPVIQLRQGLDLFANVRPCFSIDD 122
Query: 111 RLTPLKGKNKIDIVFVRENTEGLYAGAGGF-LRKGTPQEIAVQE--------------MI 155
+ P + +REN+EGLY G F L K +A + +
Sbjct: 123 QSKPF------NFCIIRENSEGLYCGFDYFPLPKAIHSLLAESQHWQTIPADEASCALRL 176
Query: 156 NTRFGVERVIRFAFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYD-LETDHY 214
++ G+ R+ FAF++A ++G +VTL DK NVL + + ++F +Q Y ++ D
Sbjct: 177 QSKSGLTRLFDFAFKHAMQTGMPRVTLADKPNVLRESGEFTRKIFESTAQRYPKIQADIL 236
Query: 215 YVDAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVH 274
VDA+A+ +I+SPE F V+V NMFGDIL+D+GA ++GGLG+A S NI + FEPVH
Sbjct: 237 NVDAVALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGLGLAPSANIG-DKGSYFEPVH 295
Query: 275 GSAPDIAGKRIANPLAAILSASMMLEYLNLEKESKWIEEAVKRAIAENK-VTPDMNGNLK 333
GS P I K ANP A L+ SM+L + +K I AV + I E + +T D+ G+
Sbjct: 296 GSGPRIR-KNCANPSAMFLTISMLLNHFGYPDRAKKIVNAVMQVIKEKRFITYDLGGHAT 354
Query: 334 TYEVGDWVVK 343
T ++ + V++
Sbjct: 355 TTDMANAVIE 364
>sp|P87257|LE3B_ASPNG 3-ISOPROPYLMALATE DEHYDROGENASE B (BETA-IPM DEHYDROGENASE B) (IMDH
B) (3-IPM-DH B) >gi|7431320|pir||S72210
3-isopropylmalate dehydrogenase (EC 1.1.1.85) B -
Aspergillus niger >gi|1911743|gb|AAB50828.1| (S83229)
beta-isopropylmalate dehydrogenase=isozyme [Aspergillus
niger, strain A733, Peptide, 371 aa]
>gi|4097257|gb|AAD10279.1| (U51131) beta-isopropylmalate
dehydrogenase [Aspergillus niger]
Length = 371
Score = 175 bits (438), Expect = 1e-42
Identities = 127/369 (34%), Positives = 188/369 (50%), Gaps = 36/369 (9%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
I V+PGDGIG EV+AE +KVL+ S + F +E G G ++ +
Sbjct: 9 ILVLPGDGIGPEVMAEAIKVLRTFNS-SSMQFHLQEELIGGISIDTHGHSVTQPVKDAAV 67
Query: 65 KFDAIYFGAIGDPRVKPGILERGI------LLKMRFELDLYVNLRPVKLYHPR------L 112
DA+ F A+G +V + RG+ LL++R +D+Y NLRP + P
Sbjct: 68 AADAVLFAAVGGSKVDH--IRRGLDGPEGGLLQVRKAMDIYANLRPCSVDVPSREIARDF 125
Query: 113 TPLKGK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFE 170
+P + + +D V VREN G Y G +K + A+ E + ++R+ R A E
Sbjct: 126 SPFRQEVIEGVDFVVVRENCGGAYFG-----KKVEEENYAMDEWGYSTTEIQRIARLAAE 180
Query: 171 YAKRSGRK-KVTLVDKANVLTYAHDLWERVFAE-VSQEY-DLETDHYYVDAMAMKMIRSP 227
A R V +DKANVL + LW RV +S EY ++ H D+ ++ M P
Sbjct: 181 LALRHDPPWPVISLDKANVLASSR-LWRRVVENTISVEYPQVKLVHQLADSASLIMATDP 239
Query: 228 ESFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--------PETTGMFEPVHGSAP 278
+ V++ N FGD+L+D ++G LG+ S +++ + G++EP HGSAP
Sbjct: 240 RVLNGVILADNTFGDMLSDQAGSLIGTLGVLPSASLDGLPHPGKQEKVRGLYEPTHGSAP 299
Query: 279 DIAGKRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEV 337
IAGK IANP A IL S+M Y N+E E++ IE+AV+ + TPD+ GN T E
Sbjct: 300 TIAGKNIANPTAMILCVSLMFRYSFNMENEARQIEDAVRAVLDRGLRTPDLGGNSSTQEF 359
Query: 338 GDWVVKFLE 346
GD VV L+
Sbjct: 360 GDAVVAALQ 368
>sp|O59930|LEU3_PHACH 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|2935579|gb|AAC26195.1| (AF050668)
3-isopropylmalate dehydrogenase [Phanerochaete
chrysosporium]
Length = 380
Score = 175 bits (438), Expect = 1e-42
Identities = 132/372 (35%), Positives = 193/372 (51%), Gaps = 30/372 (8%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELSR-VSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I ++PGDGIG EVVAE +VL+ + S V + + + FG K GE L +E
Sbjct: 7 KIVILPGDGIGPEVVAEATRVLEVVSASSSDVEIKLETHDFGGCSIDKHGEPLTAATLEA 66
Query: 63 FKKFDAIYFGAIGDPR--VKPGILERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKG 117
K DAI GAIG P+ V + LL +R L LY N+RP L +PLK
Sbjct: 67 CKLADAILLGAIGGPKWGVNSKVRPEQALLALRKALGLYANIRPANFASDSLLAYSPLKP 126
Query: 118 K--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRS 175
+DI+ +RE G Y G L ++ A MI + V+R+ R + A
Sbjct: 127 SVARGVDIIVIRELIGGAYFGERKELGARAQEDAAWDTMIYSVPEVQRITRSRRQVASPD 186
Query: 176 GRKKVTLVDKANVLTYAHDLWERVFAEVSQEY--DLETDHYYVDAMAMKMIRSPESFD-V 232
V +DKANVL + LW +V E Q L+ DH+ VD+ +M ++ +P+ + V
Sbjct: 187 PPLPVHSIDKANVLASSR-LWRKVATETIQNEFPQLKLDHHLVDSASMLIVANPKKLNGV 245
Query: 233 VVTPNMFGDILTDLGAEIVGGLGIAASGNIN-----PETTG-------MFEPVHGSAPDI 280
++T N+FGDIL+D + I G LG+ S ++ P T ++EP+HGSAPDI
Sbjct: 246 ILTENLFGDILSDESSVIPGSLGLLPSASLAARPLWPNTAHRRSSPRRVYEPIHGSAPDI 305
Query: 281 AGKRIANPLAAILSASMMLEYLNL-EKESKWIEEAVKRA-----IAENKVTPDMNGNLKT 334
AG+ IANP+ ILSA+M+ ++ +K IE AV+ A E T D+ G+ KT
Sbjct: 306 AGQGIANPIGTILSAAMLCGTRSVWRSPAKAIEAAVEGARRQGDRREGLRTADLGGSSKT 365
Query: 335 YEVGDWVVKFLE 346
++G VV+ L+
Sbjct: 366 KDIGHKVVEILK 377
>sp|O94114|LEU3_PICST 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|4099103|gb|AAD00548.1| (U83626)
beta-isopropylmalate dehydrogenase [Pichia stipitis]
Length = 373
Score = 173 bits (435), Expect = 2e-42
Identities = 127/358 (35%), Positives = 193/358 (53%), Gaps = 23/358 (6%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELS---RVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
I V+PGD +G E+ E +KVL+ +E+ + ++ FEFK + G TG LPD ++
Sbjct: 8 ITVLPGDHVGTEICNEAIKVLEAIEQATPYQKIHFEFKHHLIGGAAIDSTGVPLPDDSLA 67
Query: 62 EFKKFDAIYFGAIGDPRVKPGIL--ERGILLKMRFELDLYVNLRPVKLYHP---RLTPLK 116
K DA+ GA+G P+ G + E+G LLK+R EL+LY NLRP L+PLK
Sbjct: 68 AAKLSDAVLLGAVGGPKWGTGAVRPEQG-LLKIRKELNLYANLRPCNFASDALLELSPLK 126
Query: 117 GK--NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFA-FEYAK 173
+ + VRE G+Y G + E A + V R+ R A F +
Sbjct: 127 SEIVKGTNFTVVRELVGGIYFGERQEQEESADGESAWDTEKYSVAEVTRITRMAAFMALQ 186
Query: 174 RSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY--DLETDHYYVDAMAMKMIRSPESFD 231
+ + +DKANVL + LW + +V +E L H +D+ AM +++SP +
Sbjct: 187 HNPPLPIWSLDKANVLASSR-LWRKTVDKVMKEEFPQLTIQHQLIDSAAMILVQSPTKLN 245
Query: 232 -VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN--PETT---GMFEPVHGSAPDIAGKRI 285
+V+T NMFG I++D + I G LG+ S ++ P+T G++EP HGSAPD+ ++
Sbjct: 246 GIVITSNMFGYIISDEASVIPGSLGLLPSASLASLPDTNSAFGLYEPCHGSAPDLPENKV 305
Query: 286 ANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNGNLKTYEVGDWVV 342
NP+A ILS +MML L+ KE++ +EEAV++ + T D+ G T EVG+ VV
Sbjct: 306 -NPIATILSVAMMLRLSLDSLKEAEALEEAVRQVLDSGVRTADLRGTNSTKEVGEAVV 362
>sp|P18869|LEU3_SCHPO 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|7489952|pir||T39850 3-isopropylmalate
dehydrogenase - fission yeast (Schizosaccharomyces
pombe) >gi|11374698|pir||T43407 3-isopropylmalate
dehydrogenase (EC 1.1.1.85) [validated] - fission yeast
(Schizosaccharomyces pombe) >gi|173412|gb|AAA35316.1|
(M36910) beta-isopropylmalate dehydrogenase (leu1)
(put.); putative [Schizosaccharomyces pombe]
>gi|3393014|emb|CAA20106.1| (AL031174) 3-isopropylmalate
dehydrogenase [Schizosaccharomyces pombe]
Length = 371
Score = 171 bits (430), Expect = 8e-42
Identities = 128/376 (34%), Positives = 194/376 (51%), Gaps = 41/376 (10%)
Query: 4 RIAVIPGDGIGKEVVAEGLKVLKKLEELS-RVSFEFKEYPFGAEHYLKTGETLPDWAIEE 62
+I V+PGD IG E+VA L+VLK +E+ + EF+E+ G G L D ++
Sbjct: 5 KIVVLPGDHIGPEIVASALEVLKVVEKKRPELKLEFEEHKIGGASIDAYGTPLTDETVKA 64
Query: 63 FKKFDAIYFGAIGDPR-VKPGILERGILLKMRFELDLYVNLRPVKLYHPRL---TPLKGK 118
+ D + GA+G P P LLK+R + ++ NLRP L +PLK +
Sbjct: 65 CLEADGVLLGAVGGPEWTNPNCRPEQGLLKLRKSMGVWANLRPCNFASKSLVKYSPLKPE 124
Query: 119 --NKIDIVFVRENTEGLYAG------AGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFE 170
+D VRE T G Y G G+ P + V R+ R A
Sbjct: 125 IVEGVDFCVVRELTGGCYFGERTEDNGSGYAMDTWPYSLEE---------VSRIARLAAW 175
Query: 171 YAKRSGRKK-VTLVDKANVLTYAHDLWERVFAEV-SQEYD-LETDHYYVDAMAMKMIRSP 227
A+ S VTL+DKANVL + LW + A++ +EY L + +D+ AM +++SP
Sbjct: 176 LAETSNPPAPVTLLDKANVLATSR-LWRKTVAKIFKEEYPHLTLKNQLIDSAAMLLVKSP 234
Query: 228 ESFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN-------PETTGMFEPVHGSAPD 279
+ + VV+T N+FGDI++D + I G LG+ S +++ + + EP+HGSAPD
Sbjct: 235 RTLNGVVLTDNLFGDIISDEASVIPGSLGLLPSASLSGVVGKSEEKVHCLVEPIHGSAPD 294
Query: 280 IAGKRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKV------TPDMNGNL 332
IAGK I NP+ ILSAS++L Y LN KE++ IE AV++ + + + T D+ G
Sbjct: 295 IAGKGIVNPVGTILSASLLLRYGLNAPKEAEAIEAAVRKVLDDTSIGGRGLYTRDLGGEA 354
Query: 333 KTYEVGDWVVKFLEVV 348
T ++ VV+ LE +
Sbjct: 355 STADITKAVVEELEKI 370
>gb|AAC69609.1| (AF045154) NAD-dependent isocitrate dehydrogenase subunit 2
[Kluyveromyces lactis]
Length = 368
Score = 171 bits (430), Expect = 8e-42
Identities = 123/350 (35%), Positives = 185/350 (52%), Gaps = 22/350 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K ++ I GDG+G E+ K +K + ++V E++ + ++ T+PD A+
Sbjct: 35 KYTVSFIEGDGVGPEIS----KSVKAIFSAAKVPIEWESCDV-SPIFVNGLTTIPDPAVA 89
Query: 62 EFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKI 121
K G + P K R + L +R L+ N+RP K T + N
Sbjct: 90 SINKNLIALKGPLATPIGKG---HRSLNLTLRKTFGLFANVRPAKSIEGYKTTYENVN-- 144
Query: 122 DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVT 181
+V +RENTEG Y+G + G Q I + T+ ERVIR+AFEYA+ R KV
Sbjct: 145 -LVLIRENTEGEYSGIEHVVAPGVVQSIK----LITQDASERVIRYAFEYARAVDRSKVL 199
Query: 182 LVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD--VVVTPNM 238
+V K+ + A L+ V ++S EY D+E +D +K ++ PE++D VVV PN+
Sbjct: 200 VVHKSTIQRLADGLFVDVAKKLSSEYPDIELQTELLDNTVLKTVQHPEAYDDVVVVCPNL 259
Query: 239 FGDILTDLGAEI-VGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASM 297
+GDIL+DL + + G LG+ S NI T +FE VHGSAPDIAG+ ANP A +LS+ M
Sbjct: 260 YGDILSDLNSGLSAGSLGLTPSANIG-HTVSIFEAVHGSAPDIAGQNKANPTALLLSSVM 318
Query: 298 MLEYLNLEKESKWIEEAVKRAIAEN--KVTPDMNGNLKTYEVGDWVVKFL 345
ML ++ L + + IE+AV IA + T D+ G+ T D V++ L
Sbjct: 319 MLNHMGLTEHADKIEKAVLTTIASDAKNRTGDLGGSASTSSFTDAVIERL 368
>sp|P96197|LEU3_AZOVI 3-ISOPROPYLMALATE DEHYDROGENASE (BETA-IPM DEHYDROGENASE) (IMDH)
(3-IPM-DH) >gi|1850799|emb|CAA72151.1| (Y11280) LeuB
protein [Azotobacter vinelandii]
Length = 360
Score = 170 bits (427), Expect = 2e-41
Identities = 129/360 (35%), Positives = 186/360 (50%), Gaps = 18/360 (5%)
Query: 1 MKLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
M +I V+PGDGIG E++AE +KVL+ E ++ F G K G L D +
Sbjct: 1 MSKQILVLPGDGIGPEIMAEAVKVLQLANEKYQLGFVLGYDELGGAAVDKYGVPLADETL 60
Query: 61 EEFKK--FDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGK 118
E ++ A R +PG + F P LY P+L
Sbjct: 61 ERARRRCHPARRRRRSEMGRHRPGHPPGARPAENPFATGPVRQPAPGVLY-PQLAEASTL 119
Query: 119 NK-----IDIVFVRENTEGLYAGAGGFLRK-GTPQEIAVQEMINTRFGVERVIRFAFEYA 172
+DI+ VRE T G+Y GA R + +A + + + R+ + F+ A
Sbjct: 120 RPEVVAGLDILIVRELTGGIYFGAPRESRLLANGERMAYDTLPYSESEIRRIAKVGFDMA 179
Query: 173 KRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD 231
+ G KK+ VDKANVL + LW V EV+++Y D+ H YVD AM+++R+P+ FD
Sbjct: 180 RVRG-KKLCSVDKANVLA-SSQLWRAVVEEVAKDYPDVVLSHMYVDNAAMQLVRAPKQFD 237
Query: 232 VVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGS-APDIAGKRIANPLA 290
V+VT NMFGDIL+D + + G +G+ S +++ + GM+EP HGS PDIAGK IANPLA
Sbjct: 238 VIVTDNMFGDILSDEASMLTGSIGMLPSASLDADNKGMYEPCHGSRRPDIAGKGIANPLA 297
Query: 291 AILSASMMLEY-LNLEKESKWIEEAVKRAIAENKVTPDMNG----NLKTYEVGDWVVKFL 345
ILS SMML Y + + IE+AV + + + T D+ + T E+GD VV L
Sbjct: 298 TILSVSMMLRYSFGQVEAANAIEQAVSKVLDQGLRTGDIWSEGCRKVGTAEMGDAVVAAL 357
>gi|6324709 NAD+-dependent isocitrate dehydrogenase; Idh2p [Saccharomyces
cerevisiae] >gi|124159|sp|P28241|IDH2_YEAST ISOCITRATE
DEHYDROGENASE [NAD] SUBUNIT 2, MITOCHONDRIAL PRECURSOR
(ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|101418|pir||A39309 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) chain IDH2 precursor - yeast
(Saccharomyces cerevisiae) >gi|171747|gb|AAA34702.1|
(M74131) isocitrate dehydrogenase kinase/phosphatase
[Saccharomyces cerevisiae] >gi|1164979|emb|CAA64054.1|
(X94335) YOR3326w [Saccharomyces cerevisiae]
>gi|1420347|emb|CAA99335.1| (Z75043) ORF YOR136w
[Saccharomyces cerevisiae]
Length = 369
Score = 169 bits (425), Expect = 3e-41
Identities = 124/351 (35%), Positives = 187/351 (52%), Gaps = 24/351 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
K ++ I GDGIG E+ K +KK+ + V E++ + ++ T+PD A++
Sbjct: 36 KYTVSFIEGDGIGPEIS----KSVKKIFSAANVPIEWESCDV-SPIFVNGLTTIPDPAVQ 90
Query: 62 EFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKI 121
K G + P K R + L +R L+ N+RP K T + +
Sbjct: 91 SITKNLVALKGPLATPIGKG---HRSLNLTLRKTFGLFANVRPAKSIEGFKTTYEN---V 144
Query: 122 DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVT 181
D+V +RENTEG Y+G + G Q I + TR ERVIR+AFEYA+ GR +V
Sbjct: 145 DLVLIRENTEGEYSGIEHIVCPGVVQSIK----LITRDASERVIRYAFEYARAIGRPRVI 200
Query: 182 LVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFD--VVVTPNM 238
+V K+ + A L+ V E+S+EY DL + +D +K++ +P ++ V V PN+
Sbjct: 201 VVHKSTIQRLADGLFVNVAKELSKEYPDLTLETELIDNSVLKVVTNPSAYTDAVSVCPNL 260
Query: 239 FGDILTDLGAEI-VGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASM 297
+GDIL+DL + + G LG+ S NI + + +FE VHGSAPDIAG+ ANP A +LS+ M
Sbjct: 261 YGDILSDLNSGLSAGSLGLTPSANIGHKIS-IFEAVHGSAPDIAGQDKANPTALLLSSVM 319
Query: 298 MLEYLNLEKESKWIEEAVKRAIA---ENKVTPDMNGNLKTYEVGDWVVKFL 345
ML ++ L + I+ AV IA EN+ T D+ G T + V+K L
Sbjct: 320 MLNHMGLTNHADQIQNAVLSTIASGPENR-TGDLAGTATTSSFTEAVIKRL 369
>emb|CAA74777.1| (Y14432) NAD-dependent isocitrate dehydrogenase [Nicotiana tabacum]
Length = 357
Score = 165 bits (413), Expect = 8e-40
Identities = 119/354 (33%), Positives = 190/354 (53%), Gaps = 38/354 (10%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGET--LPDWAIEE 62
+ +IPGDG+G V +V++ ++ P E Y G+ +P+ I+
Sbjct: 30 VTLIPGDGVGPLVTDSVEQVMQAMKA-----------PVYFERYEVRGDMKCIPEEVIDS 78
Query: 63 FKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKID 122
KK G + P V G+ +L MR ELDL ++ V + P + ++ +D
Sbjct: 79 IKKNKVCLKGGLKTP-VGGGVSSLNVL--MRKELDLSASI--VHCFTFPGLPTRHED-VD 132
Query: 123 IVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTL 182
IV +RENTEG YAG + G + + V T++ ER+ +FAFEYA + RKKVT
Sbjct: 133 IVVIRENTEGEYAGLEHEVVPGVVESLKVI----TKYCSERIAKFAFEYADVNKRKKVTA 188
Query: 183 VDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
V KAN++ A L+ + EV+ +Y +++ + VD M+++ PE FDV+VTPN++G+
Sbjct: 189 VHKANIMKLADGLFLKSCREVASKYPEIQYEEIIVDNCCMQLVSRPEQFDVMVTPNLYGN 248
Query: 242 ILTDLGAEIVGGLGIAASGNINPETTGMFEPV--HGSAPDIAGK------RIANPLAAIL 293
++ + A + GG G+ GN+ G+ +PV G++P G + ANP+A +L
Sbjct: 249 LIANTAAGLAGGTGVLPGGNV-----GLIKPVFEQGASPGNVGHEKILKLKKANPVALLL 303
Query: 294 SASMMLEYLNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFLE 346
S++MML +L + +E AVKR IAE KV T D+ G+ T EV D ++ L+
Sbjct: 304 SSAMMLRHLEFPSFADRLETAVKRVIAEGKVRTKDLGGDSTTQEVVDAIIAALD 357
>gb|AAB81675.1| (AC002354) putative NAD+ dependent isocitrate dehydrogenase subunit
2, IDH2 [Arabidopsis thaliana]
Length = 367
Score = 164 bits (411), Expect = 1e-39
Identities = 124/351 (35%), Positives = 191/351 (54%), Gaps = 32/351 (9%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDG+G V +V++ + + V FE PF +K+ LP+ +E K
Sbjct: 40 VTLIPGDGVGPLVTNAVQQVMEAMH--APVYFE----PFEVHGDMKS---LPEGLLESIK 90
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK-IDI 123
K G + P V G+ + +R ELDL+ +L V ++ L L +++ +DI
Sbjct: 91 KNKVCLKGGLKTP-VGGGVSSLNV--NLRKELDLFASL--VNCFN--LPGLASRHENVDI 143
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
V +RENTEG YAG + G + + V T+F ER+ ++AFEYA + RKKVT V
Sbjct: 144 VVIRENTEGEYAGLEHEVVPGVVESLKVI----TKFCSERIAKYAFEYAYLNNRKKVTAV 199
Query: 184 DKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ A L+ EV+++Y + + VD M+++ PE FDV+VTPN++G++
Sbjct: 200 HKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDNCCMQLVARPEQFDVMVTPNLYGNL 259
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGK------RIANPLAAILSAS 296
+ + A I GG G+ GN+ E +FE G++ GK + ANP+A +LS++
Sbjct: 260 VANTAAGIAGGTGVMPGGNVGAE-YAVFE--QGASAGNVGKDTTEEQKNANPVALLLSSA 316
Query: 297 MMLEYLNLEKESKWIEEAVKRAIAE-NKVTPDMNGNLKTYEVGDWVVKFLE 346
MML +L + +E AVKR IAE N T D+ GN T EV D V+ L+
Sbjct: 317 MMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTTQEVVDAVIANLD 367
>gb|AAC49965.1| (U81994) NAD+ dependent isocitrate dehydrogenase subunit 2
[Arabidopsis thaliana]
Length = 367
Score = 164 bits (410), Expect = 2e-39
Identities = 124/351 (35%), Positives = 191/351 (54%), Gaps = 32/351 (9%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDG+G V +V++ + + V FE PF +K+ LP+ +E K
Sbjct: 40 VTLIPGDGVGPLVTNAVQQVMEAMH--APVYFE----PFEVHGDMKS---LPEGLLESIK 90
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK-IDI 123
K G + P V G+ + +R ELDL+ +L V ++ L L +++ +DI
Sbjct: 91 KNKVCLKGGLKTP-VGGGVSSLNV--NLRKELDLFASL--VNCFN--LPGLASRHENVDI 143
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
V +RENTEG YAG + G + + V T+F ER+ ++AFEYA + RKKVT V
Sbjct: 144 VVIRENTEGEYAGLEHEVVPGVVESLKVI----TKFCSERIAKYAFEYAYLNNRKKVTAV 199
Query: 184 DKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ A L+ EV+++Y + + VD M+++ PE FDV+VTPN++G++
Sbjct: 200 HKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDNCCMQLVARPEQFDVMVTPNLYGNL 259
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGK------RIANPLAAILSAS 296
+ + A I GG G+ GN+ E +FE G++ GK + ANP+A +LS++
Sbjct: 260 VANTAAGIAGGSGVMPGGNVGAE-YAVFE--QGASAGNVGKDTTEEQKNANPVALLLSSA 316
Query: 297 MMLEYLNLEKESKWIEEAVKRAIAE-NKVTPDMNGNLKTYEVGDWVVKFLE 346
MML +L + +E AVKR IAE N T D+ GN T EV D V+ L+
Sbjct: 317 MMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTTQEVVDAVIANLD 367
>sp|Q93353|IDHB_CAEEL PROBABLE ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|7497159|pir||T19810 hypothetical protein C37E2.1 -
Caenorhabditis elegans >gi|3874853|emb|CAB02822.1|
(Z81046) predicted using Genefinder~Similarity to Crab
NAD+ isocitrate dehydrogenase (TR:E124071), contains
similarity to Pfam domain: PF00180 (Isocitrate and
isopropylmalate dehydrogenases), Score=267.6,
E-value=1.1e-79, N=2~cDNA EST yk634h4.5 comes f>
Length = 379
Score = 163 bits (409), Expect = 2e-39
Identities = 112/347 (32%), Positives = 189/347 (54%), Gaps = 20/347 (5%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
KL++ +IPGDG+G E++ ++K+ + + EF+E HY ++ + A+E
Sbjct: 42 KLKVTIIPGDGVGPELIYTVQDIVKQ----TGIPIEFEEIFLSEVHYTRSSSI--ENAVE 95
Query: 62 EFKKFDAIYF-GAIGDPRV--KPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGK 118
+ + + GAI + V G L+ G+ +++R LDL+ N+ +K T +
Sbjct: 96 SIGRNNNVALKGAIEESAVLHTEGELQ-GLNMRLRRSLDLFANVVHIKTLDGIKT--RHG 152
Query: 119 NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRK 178
++D V VRE TEG Y+ L G + + I+TR ER+ +FAF+YA ++GRK
Sbjct: 153 KQLDFVIVREQTEGEYSSLEHELVPGVIECLK----ISTRTKAERIAKFAFDYATKTGRK 208
Query: 179 KVTLVDKANVLTYAHDLWERVFAEVSQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPN 237
KVT V KAN++ L+ R V+++Y ++ + +D M+++ PE FDV+V PN
Sbjct: 209 KVTAVHKANIMKLGDGLFLRTCEGVAKQYPKIQFESMIIDNTCMQLVSKPEQFDVMVMPN 268
Query: 238 MFGDILTDLGAEIVGGLGIAASGNINPETTGMFEP-VHGSAPDIAGKRIANPLAAILSAS 296
++G+I+ +L A +VGG G+ ++ + +FEP S + G+ IANP A IL A+
Sbjct: 269 LYGNIIDNLAAGLVGGAGVVPGQSVGRDFV-IFEPGSRHSFQEAMGRSIANPTAMILCAA 327
Query: 297 MMLEYLNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVV 342
ML +L+L+ + +AV + E KV T D+ G T + D V+
Sbjct: 328 NMLNHLHLDAWGNSLRQAVADVVKEGKVRTRDLGGYATTVDFADAVI 374
>gb|AAB63461.1| (AF009036) NAD(+)-isocitrate dehydrogenase subunit I [Ajellomyces
capsulatus]
Length = 388
Score = 161 bits (404), Expect = 9e-39
Identities = 118/339 (34%), Positives = 178/339 (51%), Gaps = 18/339 (5%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPF-GAEHYLKTGETLPDWAI 60
K + +IPGDGIG EV AE +K + K + V E+++ G + K E L +I
Sbjct: 51 KYTVTLIPGDGIGTEV-AESVKTIFKADN---VPIEWEQVDVSGLDAGNKHSEDLFKESI 106
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
K+ G + P + G + + +R ELD+Y ++ +K T +
Sbjct: 107 ASLKRNKLGLKGILHTPVERSG--HQSFNVALRQELDIYASIVLIKNIPGYKTR---HDN 161
Query: 121 IDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKV 180
+D+ +RENTEG Y+G L + + I TR ER+ +FAF +A + RKKV
Sbjct: 162 VDLCIIRENTEGEYSG----LEHQSVSGVVESLKIITRAKSERIAKFAFSFALANNRKKV 217
Query: 181 TLVDKANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMF 239
T + KAN++ A L+ F +V++ Y LET+ VD +M+ + P+ FDV+V PN++
Sbjct: 218 TCIHKANIMKLADGLFRSTFHKVAESYPTLETNDMIVDNASMQAVARPQQFDVMVMPNLY 277
Query: 240 GDILTDLGAEIVGGLGIAASGNINPETTGMFEP-VHGSAPDIAGKRIANPLAAILSASMM 298
G IL+++GA +VGG GI N+ + +FEP DI GK ANP A ILS SM+
Sbjct: 278 GGILSNVGAALVGGPGIVPGCNMGRD-VAVFEPGCRHVGLDIKGKDQANPTALILSGSML 336
Query: 299 LEYLNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYE 336
L +L L++ + I +AV I E T DM G T+E
Sbjct: 337 LRHLGLDEHANRISKAVYDVIGEGVTRTRDMGGQASTHE 375
>sp|Q59985|IDH_STRSL ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCINATE DECARBOXYLASE)
(IDH) (NADP+-SPECIFIC ICDH) (IDP)
>gi|1280403|gb|AAA98355.1| (L14780) isocitrate
dehydrogenase [Streptococcus salivarius]
Length = 391
Score = 161 bits (402), Expect = 2e-38
Identities = 117/377 (31%), Positives = 182/377 (48%), Gaps = 38/377 (10%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELS---RVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
I I GDG+G ++ + K E++ + E++E G + Y KTGE LP +E
Sbjct: 20 IPFIEGDGVGHDIWKNAQAIFDKAVEVAYEGKRHIEWQELLAGKKAYDKTGEWLPKETLE 79
Query: 62 EFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKI 121
++ G + P V GI R + + +R ELDLY +RPV+ + +PLK K
Sbjct: 80 AIRESLVAIKGPLETP-VGGGI--RSLNVALRQELDLYACVRPVRYFDGVASPLKEPEKT 136
Query: 122 DIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGV-------------------- 161
+I RENTE +YAG GT V E + T V
Sbjct: 137 NITIFRENTEDIYAGIEW--EAGTADVKRVIEFLQTEMNVNKIRFPESSSIGIKPISIEG 194
Query: 162 -ERVIRFAFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY--------DLETD 212
+R+IR A +YA ++ KKVTLV K N+ + + + EV+QE LE +
Sbjct: 195 SKRLIRSAIDYALKNNLKKVTLVHKGNIQKFTEGGFRKWGYEVAQEDYKEELLAGRLEIN 254
Query: 213 HYYVDAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETT-GMFE 271
D +++ +PE FDVV N+ GD +D A VGG+GI+ NIN +T +FE
Sbjct: 255 DIIADNFLQQILLNPEKFDVVALTNLNGDYASDALAAQVGGIGISPGANINYQTGHAIFE 314
Query: 272 PVHGSAPDIAGKRIANPLAAILSASMMLEYLNLEKESKWIEEAVKRAIAENKVTPDMNGN 331
HG+APDIA + ANP + +LS M+L+Y+ + ++ I A+++ + T D+
Sbjct: 315 ATHGTAPDIADQDKANPCSVLLSGCMLLDYIGWTEAAQLITSAIEKTFKADIFTADLAFG 374
Query: 332 LKTYEVGDWVVKFLEVV 348
+ Y + + L ++
Sbjct: 375 KQAYSTSAFSNQILSIM 391
>pir||T06131 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) chain 1 - Arabidopsis
thaliana >gi|3080424|emb|CAA18743.1| (AL022604) NAD+
dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] >gi|7270478|emb|CAB80243.1|
(AL161587) NAD+ dependent isocitrate dehydrogenase
subunit 1 [Arabidopsis thaliana]
Length = 367
Score = 160 bits (401), Expect = 2e-38
Identities = 118/349 (33%), Positives = 185/349 (52%), Gaps = 28/349 (8%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG V ++++ E F++Y E +P +E +
Sbjct: 40 VTLIPGDGIGPLVT----NAVEQVMEAMHAPIFFEKYDVHGEM-----SRVPPEVMESIR 90
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
K G + P V G+ + ++R ELDL+ +L V ++ P + +N +DIV
Sbjct: 91 KNKVCLKGGLKTP-VGGGVSSLNV--QLRKELDLFASL--VNCFNLPGLPTRHEN-VDIV 144
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RENTEG YAG + G + + V T+F ER+ ++AFEYA + RKKVT V
Sbjct: 145 VIRENTEGEYAGLEHEVVPGVVESLKVI----TKFCSERIAKYAFEYAYLNNRKKVTAVH 200
Query: 185 KANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ A L+ EV+++Y T + VD M+++ PE FDV+VTPN++G+++
Sbjct: 201 KANIMKLADGLFLESCREVAKKYPSITYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLV 260
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRI-----ANPLAAILSASMM 298
+ A I GG G+ GN+ + +FE SA ++ +I ANP+A +LS++MM
Sbjct: 261 ANTAAGIAGGTGVMPGGNVGAD-HAVFEQ-GASAGNVGKDKIVLENKANPVALLLSSAMM 318
Query: 299 LEYLNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFLE 346
L +L + +E AVK+ IAE K T D+ G T EV D V+ L+
Sbjct: 319 LRHLQFPSFADRLETAVKKVIAEGKCRTKDLGGTSTTQEVVDAVIAKLD 367
>emb|CAA74776.1| (Y14431) NAD-dependent isocitrate dehydrogenase [Nicotiana tabacum]
Length = 371
Score = 159 bits (398), Expect = 5e-38
Identities = 112/349 (32%), Positives = 188/349 (53%), Gaps = 28/349 (8%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG V ++++ + F+ Y + +++P +E +
Sbjct: 44 VTLIPGDGIGPLVTG----AVEQVMDAMHAPVYFERYDVHGDM-----KSVPPEVMESIR 94
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
K G + P V G+ + ++R ELDLY +L V ++ + P + +N +DIV
Sbjct: 95 KNKVCLKGGLKTP-VGGGVSSLNV--QLRKELDLYASL--VHCFNLQGLPTRHEN-VDIV 148
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RENTEG Y+G + G + + V T+F ER+ ++AFEYA + RK +T V
Sbjct: 149 VIRENTEGEYSGLEHEVVPGVVESLKVM----TKFCSERIAKYAFEYAYLNNRKVLTAVH 204
Query: 185 KANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ A L+ EV+ +Y ++ + VD M+++ PE FDV+VTPN++G+++
Sbjct: 205 KANIMKLADGLFLESCPEVATKYPGIKYNEIIVDNCCMQLVSRPEQFDVMVTPNLYGNLV 264
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRI-----ANPLAAILSASMM 298
+ A I GG G+ GN+ + +FE SA ++ ++I ANP+A LS++MM
Sbjct: 265 ANTAAGIAGGTGVMPGGNVGAD-HAVFEQ-GASAGNVGNEKILEQKKANPVALFLSSAMM 322
Query: 299 LEYLNLEKESKWIEEAVKRAIAENK-VTPDMNGNLKTYEVGDWVVKFLE 346
L +L + ++ +VKR IAE K +T D+ GN T E+ D V+ L+
Sbjct: 323 LRHLQFPSFADRLKTSVKRVIAEGKYMTKDLGGNCTTQEITDAVIANLD 371
>emb|CAC11264.1| (AL445063) probable isocitrate dehydrogenase [Thermoplasma
acidophilum]
Length = 405
Score = 159 bits (397), Expect = 6e-38
Identities = 112/368 (30%), Positives = 184/368 (49%), Gaps = 47/368 (12%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELS---RVSFEFKEYPFGAEHYLKTGETLPDW 58
++ I I GDGIG ++ + VL +++ + S E+K+ G E Y KTG+ LP
Sbjct: 17 RVTIGYIEGDGIGPDISKTTINVLNAALDIAYGGKRSIEWKKILAGDEAYDKTGDRLPQS 76
Query: 59 AIEEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGK 118
+++E KK G + P + G R + + +R LDLY N+RPV+ + +P+
Sbjct: 77 SLDELKKCVVSLKGPLTTP-IGEGF--RSLNVTLRQYLDLYANIRPVRYFPGVPSPVVHP 133
Query: 119 NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVE---------------- 162
+++V RENTE LYAG R + V+E + + F +
Sbjct: 134 EYMNMVVFRENTEDLYAGIEW--RYDSDGAKKVREFLASTFNINLTSDTGIGIKPISKFK 191
Query: 163 --RVIRFAFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQ-----------EYDL 209
R++R A +YA + RK VTLV K N++ Y ++ EV++ EY
Sbjct: 192 STRLVRKAIQYAIANKRKSVTLVHKGNIMKYTEGAFKEWGYEVAKTEFGNYTVTETEYLQ 251
Query: 210 ETDHY---------YVDAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASG 260
+ + Y D M +++ + +DV+ TPN+ GD L+D A VGG+G+A
Sbjct: 252 DPEKYSNKIVIKDRITDNMFQQVLTRTQEYDVIATPNLNGDYLSDALAAQVGGIGLAPGA 311
Query: 261 NINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLNLEKESKWIEEAVKRAIA 320
NI + +FE VHG+AP AG +ANP + ILS MML+++ + + ++EA+ +
Sbjct: 312 NIG-DHYAVFEAVHGTAPKYAGMDVANPTSLILSGEMMLQHIGWNEAANILDEAIMQTYR 370
Query: 321 ENKVTPDM 328
+ K+T D+
Sbjct: 371 DQKLTQDI 378
>pir||T04670 isocitrate dehydrogenase (NAD+) homolog F8D20.160 - Arabidopsis
thaliana >gi|3367583|emb|CAA20035.1| (AL031135) NAD+
dependent isocitrate dehydrogenase -like protein
[Arabidopsis thaliana] >gi|7270516|emb|CAB80281.1|
(AL161587) NAD+ dependent isocitrate dehydrogenase-like
protein [Arabidopsis thaliana]
Length = 368
Score = 158 bits (396), Expect = 8e-38
Identities = 116/349 (33%), Positives = 183/349 (52%), Gaps = 28/349 (8%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG V ++++ E F+ Y L +P+ IE K
Sbjct: 41 VTLIPGDGIGPLVTG----AVEQVMEAMHAPVHFERY-----EVLGNMRKVPEEVIESVK 91
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
+ G + P V G+ + +++R ELD++ +L V + + +N +DIV
Sbjct: 92 RNKVCLKGGLATP-VGGGV--SSLNMQLRKELDIFASL--VNCINVPGLVTRHEN-VDIV 145
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RENTEG Y+G + G + + V T+F ER+ R+AFEYA + RKKVT V
Sbjct: 146 VIRENTEGEYSGLEHEVVPGVVESLKVI----TKFCSERIARYAFEYAYLNNRKKVTAVH 201
Query: 185 KANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ A L+ EV++ Y + + VD M+++ PE FDV+VTPN++G+++
Sbjct: 202 KANIMKLADGLFLESCREVAKHYSGITYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLI 261
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRI-----ANPLAAILSASMM 298
+ A I GG G+ GN+ E +FE SA ++ ++ ANP+A +LS++MM
Sbjct: 262 ANTAAGIAGGTGVMPGGNVGAE-HAIFEQ-GASAGNVGNDKMVEQKKANPVALLLSSAMM 319
Query: 299 LEYLNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFLE 346
L +L + +E AVK+ I E K T D+ G+ T EV D V+ LE
Sbjct: 320 LRHLRFPTFADRLETAVKQVIKEGKYRTKDLGGDCTTQEVVDAVIAALE 368
>sp|Q28479|IDHB_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|2134531|pir||S58663 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) beta chain - crab-eating macaque
(fragment) >gi|1182009|emb|CAA57954.1| (X82632)
NAD+-isocitrate dehydrogenase [Macaca fascicularis]
Length = 381
Score = 158 bits (395), Expect = 1e-37
Identities = 106/343 (30%), Positives = 178/343 (50%), Gaps = 15/343 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDG+G E+ + +K++ + + V EF+E+ + + E L + + K
Sbjct: 49 VTMLPGDGVGPEL----MHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKL-EQVLSSMK 103
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
+ G I P G L +++R +LDL+ N+ VK +T N +D+V
Sbjct: 104 ENKVAIIGKIHTPMEYKGELA-SYDMRLRRKLDLFANVVHVKSLPGYMTR---HNNLDLV 159
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RE TEG Y+ +G + + + TR +R+ +FAF+YA + GR KVT V
Sbjct: 160 IIREQTEGEYSSLEHESARGVIECLKIV----TRAKSQRIAKFAFDYATKKGRSKVTAVH 215
Query: 185 KANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ L+ + EV++ Y ++ + +D M+++++P FDV+V PN++G+I+
Sbjct: 216 KANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNII 275
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+L A +VGG G+ + + E G+ IANP A +LSAS ML +LN
Sbjct: 276 DNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLN 335
Query: 304 LEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFL 345
LE S I +AVK+ I KV T DM G ++ + V+ L
Sbjct: 336 LEYHSNMIADAVKKVIKVGKVRTSDMGGYATCHDFTEAVIAAL 378
>gb|AAD09339.1| (AF023265) NAD+-specific isocitrate dehydrogenase beta subunit
isoform A [Homo sapiens]
Length = 383
Score = 157 bits (393), Expect = 2e-37
Identities = 106/343 (30%), Positives = 178/343 (50%), Gaps = 15/343 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDG+G E+ + +K++ + + V EF+E+ + + E L + + K
Sbjct: 51 VTMLPGDGVGPEL----MHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKL-EQVLSSMK 105
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
+ G I P G L +++R +LDL+ N+ VK +T N +D+V
Sbjct: 106 ENKVAIIGKIHTPMEYKGELA-SYDMRLRRKLDLFANVVHVKSLPGYMTR---HNNLDLV 161
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RE TEG Y+ +G + + + TR +R+ +FAF+YA + GR KVT V
Sbjct: 162 IIREQTEGEYSSLEHESARGVIECLKIV----TRAKSQRIAKFAFDYATKKGRGKVTAVH 217
Query: 185 KANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ L+ + EV++ Y ++ + +D M+++++P FDV+V PN++G+I+
Sbjct: 218 KANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNII 277
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+L A +VGG G+ + + E G+ IANP A +LSAS ML +LN
Sbjct: 278 DNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLN 337
Query: 304 LEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFL 345
LE S I +AVK+ I KV T DM G ++ + V+ L
Sbjct: 338 LEYHSSMIADAVKKVIKVGKVRTSDMGGYATCHDFTEAVIAAL 380
>emb|CAC01443.1| (AL049712) dJ686C3.1.1 (isocitrate dehydrogenase 3 (NAD+) beta
(isoform A)) [Homo sapiens]
Length = 383
Score = 157 bits (393), Expect = 2e-37
Identities = 106/343 (30%), Positives = 178/343 (50%), Gaps = 15/343 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDG+G E+ + +K++ + + V EF+E+ + + E L + + K
Sbjct: 51 VTMLPGDGVGPEL----MHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKL-EQVLSSMK 105
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
+ G I P G L +++R +LDL+ N+ VK +T N +D+V
Sbjct: 106 ENKVAIIGKIHTPMEYKGELA-SYDMRLRRKLDLFANVVHVKSLPGYMTR---HNNLDLV 161
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RE TEG Y+ +G + + + TR +R+ +FAF+YA + GR KVT V
Sbjct: 162 IIREQTEGEYSSLEHESARGVIECLKIV----TRAKSQRIAKFAFDYATKKGRGKVTAVH 217
Query: 185 KANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ L+ + EV++ Y ++ + +D M+++++P FDV+V PN++G+I+
Sbjct: 218 KANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNII 277
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+L A +VGG G+ + + E G+ IANP A +LSAS ML +LN
Sbjct: 278 DNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLN 337
Query: 304 LEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFL 345
LE S I +AVK+ I KV T DM G ++ + V+ L
Sbjct: 338 LEYHSSMIADAVKKVIKVGKVRTSDMGGYATCHDFTEAVIAAL 380
>gi|5901982 isocitrate dehydrogenase 3 (NAD+) beta [Homo sapiens]
>gi|6166246|sp|O43837|IDHB_HUMAN ISOCITRATE
DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|2737886|gb|AAB94295.1| (U49283) NAD+-specific
isocitrate dehydrogenase beta precursor [Homo sapiens]
Length = 385
Score = 156 bits (390), Expect = 4e-37
Identities = 107/344 (31%), Positives = 178/344 (51%), Gaps = 15/344 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDG+G E+ + +K++ + + V EF+E+ + + E L + + K
Sbjct: 51 VTMLPGDGVGPEL----MHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKL-EQVLSSMK 105
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
+ G I P G L +++R +LDL+ N+ VK +T N +D+V
Sbjct: 106 ENKVAIIGKIHTPMEYKGELA-SYDMRLRRKLDLFANVVHVKSLPGYMTR---HNNLDLV 161
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RE TEG Y+ +G + + + TR +R+ +FAF+YA + GR KVT V
Sbjct: 162 IIREQTEGEYSSLEHESARGVIECLKIV----TRAKSQRIAKFAFDYATKKGRGKVTAVH 217
Query: 185 KANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ L+ + EV++ Y ++ + +D M+++++P FDV+V PN++G+I+
Sbjct: 218 KANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNII 277
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+L A +VGG G+ + + E G+ IANP A +LSAS ML +LN
Sbjct: 278 DNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLN 337
Query: 304 LEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFLE 346
LE S I +AVK+ I KV T DM G T + V+ L+
Sbjct: 338 LEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQ 381
>pir||T13147 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) beta chain isoform B
- human >gi|4103448|gb|AAD09340.1| (AF023266)
NAD+-specific isocitrate dehydrogenase beta subunit
isoform B [Homo sapiens]
Length = 387
Score = 156 bits (390), Expect = 4e-37
Identities = 107/344 (31%), Positives = 178/344 (51%), Gaps = 15/344 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDG+G E+ + +K++ + + V EF+E+ + + E L + + K
Sbjct: 51 VTMLPGDGVGPEL----MHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKL-EQVLSSMK 105
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
+ G I P G L +++R +LDL+ N+ VK +T N +D+V
Sbjct: 106 ENKVAIIGKIHTPMEYKGELA-SYDMRLRRKLDLFANVVHVKSLPGYMTR---HNNLDLV 161
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RE TEG Y+ +G + + + TR +R+ +FAF+YA + GR KVT V
Sbjct: 162 IIREQTEGEYSSLEHESARGVIECLKIV----TRAKSQRIAKFAFDYATKKGRGKVTAVH 217
Query: 185 KANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ L+ + EV++ Y ++ + +D M+++++P FDV+V PN++G+I+
Sbjct: 218 KANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNII 277
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+L A +VGG G+ + + E G+ IANP A +LSAS ML +LN
Sbjct: 278 DNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLN 337
Query: 304 LEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFLE 346
LE S I +AVK+ I KV T DM G T + V+ L+
Sbjct: 338 LEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQ 381
>emb|CAC01442.1| (AL049712) dJ686C3.1.2 (isocitrate dehydrogenase 3 (NAD+) beta
(isoform B)) [Homo sapiens]
Length = 387
Score = 156 bits (390), Expect = 4e-37
Identities = 107/344 (31%), Positives = 178/344 (51%), Gaps = 15/344 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDG+G E+ + +K++ + + V EF+E+ + + E L + + K
Sbjct: 51 VTMLPGDGVGPEL----MHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKL-EQVLSSMK 105
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
+ G I P G L +++R +LDL+ N+ VK +T N +D+V
Sbjct: 106 ENKVAIIGKIHTPMEYKGELA-SYDMRLRRKLDLFANVVHVKSLPGYMTR---HNNLDLV 161
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RE TEG Y+ +G + + + TR +R+ +FAF+YA + GR KVT V
Sbjct: 162 IIREQTEGEYSSLEHESARGVIECLKIV----TRAKSQRIAKFAFDYATKKGRGKVTAVH 217
Query: 185 KANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ L+ + EV++ Y ++ + +D M+++++P FDV+V PN++G+I+
Sbjct: 218 KANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNII 277
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+L A +VGG G+ + + E G+ IANP A +LSAS ML +LN
Sbjct: 278 DNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLN 337
Query: 304 LEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFLE 346
LE S I +AVK+ I KV T DM G T + V+ L+
Sbjct: 338 LEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQ 381
>gi|11421208 isocitrate dehydrogenase 3 (NAD+) beta [Homo sapiens]
Length = 385
Score = 156 bits (390), Expect = 4e-37
Identities = 107/344 (31%), Positives = 178/344 (51%), Gaps = 15/344 (4%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDG+G E+ + +K++ + + V EF+E+ + + E L + + K
Sbjct: 51 VTMLPGDGVGPEL----MHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKL-EQVLSSMK 105
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
+ G I P G L +++R +LDL+ N+ VK +T N +D+V
Sbjct: 106 ENKVAIIGKIHTPMEYKGELA-SYDMRLRRKLDLFANVVHVKSLPGYMTR---HNNLDLV 161
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RE TEG Y+ +G + + + TR +R+ +FAF+YA + GR KVT V
Sbjct: 162 IIREQTEGEYSSLEHESARGVIECLKIV----TRAKSQRIAKFAFDYATKKGRGKVTAVH 217
Query: 185 KANVLTYAHDLWERVFAEVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDIL 243
KAN++ L+ + EV++ Y ++ + +D M+++++P FDV+V PN++G+I+
Sbjct: 218 KANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNII 277
Query: 244 TDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLN 303
+L A +VGG G+ + + E G+ IANP A +LSAS ML +LN
Sbjct: 278 DNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLN 337
Query: 304 LEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFLE 346
LE S I +AVK+ I KV T DM G T + V+ L+
Sbjct: 338 LEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQ 381
>pir||T37546 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) - fission
yeast (Schizosaccharomyces pombe)
>gi|2408008|emb|CAB16208.1| (Z99161) putative isocitrate
dehydrogenase (EC 1.1.1.41) [Schizosaccharomyces pombe]
Length = 356
Score = 155 bits (389), Expect = 5e-37
Identities = 116/350 (33%), Positives = 177/350 (50%), Gaps = 23/350 (6%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPF-GAEHYLKTGETLPDWAI 60
K + +IPGDGIG+E ++ K + V EF+E G E K+ AI
Sbjct: 20 KYTVTLIPGDGIGRETSNAVTEIFKT----ANVPIEFEEIDVTGMEKNNKSSGDALHEAI 75
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN- 119
+ K+ G + P K G + L R ELD+Y +L +K + K ++
Sbjct: 76 QSLKRNKVGLKGILFTPFEKGGHTSFNVAL--RKELDIYASLVLIK----NIPGFKTRHD 129
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKK 179
+D +RENTEG Y+G L + + I T + +R+ +FAF++A ++GRK
Sbjct: 130 NVDFAIIRENTEGEYSG----LEHQSVPGVVESLKIITEYKSKRIAQFAFDFALQNGRKS 185
Query: 180 VTLVDKANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNM 238
VT + KAN++ A L+ R F +V+ YD T VD +M+ + P+ FDV+V PN+
Sbjct: 186 VTCIHKANIMKLADGLFRRTFYDVANGYDAITPKDLIVDNASMQAVSRPQQFDVLVMPNL 245
Query: 239 FGDILTDLGAEIVGGLGIAASGNINPETTGMFEP-VHGSAPDIAGKRIANPLAAILSASM 297
+G IL+++G+ +VGG G+ N + +FEP I G+ ANP AAILSA +
Sbjct: 246 YGSILSNIGSALVGGPGVIPGANFGRD-YALFEPGCRHVGLSITGRGEANPTAAILSACL 304
Query: 298 MLEYLNLEKESKWIEEAVKRAIAENK-VTPDMNGNLKTYEVGDWVVKFLE 346
ML +L L+ + I A I E K +T D+ G+ T GD+ LE
Sbjct: 305 MLRHLGLKDYADLINAATYSVIEEGKTLTKDLGGSAST---GDFTHAILE 351
>gi|6324291 alpha-4-beta-4 subunit of mitochondrial isocitrate dehydrogenase 1;
Idh1p [Saccharomyces cerevisiae]
>gi|400041|sp|P28834|IDH1_YEAST ISOCITRATE DEHYDROGENASE
[NAD] SUBUNIT 1, MITOCHONDRIAL PRECURSOR (ISOCITRIC
DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|320779|pir||S31264 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) chain IDH1 precursor - yeast
(Saccharomyces cerevisiae) >gi|171766|gb|AAA34711.1|
(M95203) isocitrate dehydrogenase-1 nuclear encoded
[Saccharomyces cerevisiae] >gi|1301886|emb|CAA95904.1|
(Z71313) ORF YNL037c [Saccharomyces cerevisiae]
Length = 360
Score = 155 bits (387), Expect = 9e-37
Identities = 114/356 (32%), Positives = 181/356 (50%), Gaps = 32/356 (8%)
Query: 2 KLRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAE--HYLKTGETLPDW- 58
+ + +IPGDG+GKE+ + R FE + P E + +T +
Sbjct: 28 RFTVTLIPGDGVGKEIT-----------DSVRTIFEAENIPIDWETINIKQTDHKEGVYE 76
Query: 59 AIEEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGK 118
A+E K+ G P + G + + +R +LD+Y N+ K T +
Sbjct: 77 AVESLKRNKIGLKGLWHTPADQTG--HGSLNVALRKQLDIYANVALFKSLKGVKTRIPD- 133
Query: 119 NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRK 178
ID++ +RENTEG ++G G + + V TR ER+ RFAF++AK+ RK
Sbjct: 134 --IDLIVIRENTEGEFSGLEHESVPGVVESLKVM----TRPKTERIARFAFDFAKKYNRK 187
Query: 179 KVTLVDKANVLTYAHDLWERVFAEVSQ-EY-DLETDHYYVDAMAMKMIRSPESFDVVVTP 236
VT V KAN++ L+ + E+ Q EY D++ VD +M+ + P FDV+VTP
Sbjct: 188 SVTAVHKANIMKLGDGLFRNIITEIGQKEYPDIDVSSIIVDNASMQAVAKPHQFDVLVTP 247
Query: 237 NMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGS---APDIAGKRIANPLAAIL 293
+M+G IL ++GA ++GG G+ A N + +FEP GS DI G+ +ANP A IL
Sbjct: 248 SMYGTILGNIGAALIGGPGLVAGANFGRD-YAVFEP--GSRHVGLDIKGQNVANPTAMIL 304
Query: 294 SASMMLEYLNLEKESKWIEEAVKRAIAENK-VTPDMNGNLKTYEVGDWVVKFLEVV 348
S+++ML +L L + + I +AV IAE K T D+ G+ T + + ++ L +
Sbjct: 305 SSTLMLNHLGLNEYATRISKAVHETIAEGKHTTRDIGGSSSTTDFTNEIINKLSTM 360
>pir||A72658 probable isocitrate dehydrogenase APE0689 - Aeropyrum pernix
(strain K1) >gi|5104350|dbj|BAA79665.1| (AP000060) 435aa
long hypothetical isocitrate dehydrogenase [Aeropyrum
pernix]
Length = 435
Score = 153 bits (383), Expect = 3e-36
Identities = 119/391 (30%), Positives = 191/391 (48%), Gaps = 55/391 (14%)
Query: 5 IAVIPGDGIGKEVVAEGLKVL----KKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAI 60
+A I GDG+G EVV LKV+ KK+ SR + E G K GE LP +
Sbjct: 38 VAFIRGDGVGPEVVESALKVVDAAVKKVYGGSR-RIVWWELLAGHLAREKCGELLPKATL 96
Query: 61 EEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK 120
E + G + P G R + + +R LDLY N+RPV+ Y+ + P K ++
Sbjct: 97 EGIRLARVALKGPLETP---VGTGYRSLNVAIRQALDLYANIRPVR-YYGQPAPHKYADR 152
Query: 121 IDIVFVRENTEGLYAGA--------GGFLRKGTPQEIAVQEMINTRFGVERVIRFA---- 168
+D+V RENTE +YAG +R+ +E + + GV+ + RFA
Sbjct: 153 VDMVIFRENTEDVYAGIEWPHDSPEAARIRRFLAEEFGISIREDAGIGVKPISRFATRRL 212
Query: 169 ----FEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEY-------DLETDHYY-- 215
E+A R+G VT++ K N++ Y + R EV+ E + E Y
Sbjct: 213 MERALEWALRNGNTVVTIMHKGNIMKYTEGAFMRWAYEVALEKFREHVVTEQEVQEKYGG 272
Query: 216 -------------VDAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNI 262
D M ++I P + V+V PN+ GD ++D + +VGG+G+AA N+
Sbjct: 273 VRPEGKILVNDRIADNMLQQIITRPWDYQVIVAPNLNGDYISDAASALVGGIGMAAGMNM 332
Query: 263 NPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML-EYLNLEKESKWIEEAVKRAIAE 321
+ + EPVHG+AP AGK + NP A ILSAS+++ E++ + +E A+++A+
Sbjct: 333 G-DGIAVAEPVHGTAPKYAGKDLINPSAEILSASLLIGEFMGWREVKSIVEYAIRKAVQS 391
Query: 322 NKVTPDMNGN------LKTYEVGDWVVKFLE 346
KVT D+ + L+T E + ++ +++
Sbjct: 392 KKVTQDLARHMPGVQPLRTSEYTETLIAYID 422
>gb|AAC69608.1| (AF045153) NAD-dependent isocitrate dehydrogenase subunit 1
[Kluyveromyces lactis]
Length = 361
Score = 150 bits (374), Expect = 3e-35
Identities = 111/347 (31%), Positives = 178/347 (50%), Gaps = 26/347 (7%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDG+GKEV +K+ E + +++ L+ E + A+E K
Sbjct: 32 VTLIPGDGVGKEVTDSVVKIF----ENENIPIDWETIDISG---LENTENVQR-AVESLK 83
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIV 124
+ G P + G + + +R +LD++ N+ K T L N ID+V
Sbjct: 84 RNKVGLKGIWHTPADQTG--HGSLNVALRKQLDIFANVALFKSIPGVKTRL---NNIDMV 138
Query: 125 FVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVD 184
+RENTEG Y+G G + + + TR ER+ RFAF++A ++ RK V V
Sbjct: 139 IIRENTEGEYSGLEHESVPGVVESLKIM----TRAKSERIARFAFDFALKNNRKSVCAVH 194
Query: 185 KANVLTYAHDLWERVFAEV-SQEY-DLETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ L+ E+ + EY +L+ + VD +M+ + P FDV+VTPN++G I
Sbjct: 195 KANIMKLGDGLFRNTVNEIGANEYPELDVKNIIVDNASMQAVAKPHQFDVLVTPNLYGSI 254
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGS---APDIAGKRIANPLAAILSASMML 299
L ++G+ ++GG G+ N E +FEP GS DI G+ +ANP A ILS+++ML
Sbjct: 255 LGNIGSALIGGPGLVPGANFGRE-YAVFEP--GSRHVGLDIKGQNVANPTAMILSSTLML 311
Query: 300 EYLNLEKESKWIEEAVKRAIAENK-VTPDMNGNLKTYEVGDWVVKFL 345
+L L + I +A I+E K T D+ G+ T E + V++ L
Sbjct: 312 RHLGLNAYADRISKATYDVISEGKSTTRDIGGSASTSEFTNAVIEKL 358
>gb|AAD55084.1| (AF176568) isocitrate dehydrogenase gamma subunit [Strongyloides
stercoralis]
Length = 388
Score = 148 bits (369), Expect = 1e-34
Identities = 110/352 (31%), Positives = 186/352 (52%), Gaps = 20/352 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ ++PGDGIG E+ L +++K+ + V +F+E ++ + E+ D AI +
Sbjct: 47 VTLLPGDGIGPEM----LDIVQKIFTFANVPVDFEECQLDSKP--TSPESDLDNAIMSIQ 100
Query: 65 KFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNK-IDI 123
+ G I P R + L+ R LDL+ N+ L+ + + ++K I+I
Sbjct: 101 RNGVAIKGNIETKYDDPIFKSRNVELRRR--LDLFANV----LHCVSIPTIPTRHKGINI 154
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
V +RENTEG Y+G G + + I TR +ER+ RFAF+YA RKK+T V
Sbjct: 155 VMIRENTEGEYSGHEHETVNGVVESLK----ICTREKLERISRFAFDYAIAHNRKKITAV 210
Query: 184 DKANVLTYAHDLWERVFAEVSQ-EYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNMFGD 241
KAN+ A L+ +V E+++ EY T D VD +M+++ P+ FD+++ PN++G+
Sbjct: 211 HKANIQKLADGLFLKVVGEIAEKEYPSITFDSMIVDNASMQLVSRPQQFDIMLMPNLYGN 270
Query: 242 ILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEY 301
I++++ +VGG G+ + NI + + IAG+ IANP A I + ML Y
Sbjct: 271 IISNIACGLVGGPGLVSGMNIGDKYAVFETGTRNTGSGIAGQDIANPTAFIRAGVDMLHY 330
Query: 302 LNLEKESKWIEEAVKRAIAENKV-TPDMNGNLKTYEVGDWVVKFLEVVANEE 352
L L + + I +A+ AI ++ T D+ G KT +V + V++ +E + E
Sbjct: 331 LGLTEYANLISDALFDAIVIKRLHTKDIGGTAKTSDVVNAVMRNIESFSKSE 382
>dbj|BAA13177.2| (D86855) isocitrate dehydrogenase [Caldococcus noboribetus]
Length = 429
Score = 148 bits (369), Expect = 1e-34
Identities = 120/370 (32%), Positives = 179/370 (47%), Gaps = 51/370 (13%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFE---FKEYPFGAEHYLKTGETLPDWA 59
L +A GDGIG E+V KVL + + + E G E + G LPD
Sbjct: 33 LIVAYFKGDGIGPEIVESAKKVLDAAVDKAYGGTRRIVWWEVTAGEEAQKECGSLLPDGT 92
Query: 60 IEEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN 119
++ FK G + P V G R + + +R LDLY N+RPVK Y + TP
Sbjct: 93 LQAFKLARVNLKGPLTTP-VGGGF--RSLNVTLRMVLDLYSNVRPVKWYG-QPTPHCHPE 148
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVE----------------- 162
ID V RENTE +YAG +P+ +++ + FG+E
Sbjct: 149 NIDWVIFRENTEDVYAGIEWPF--DSPEAQKIRDFLKKEFGIELTPDTGIGIKPISKWRT 206
Query: 163 -RVIRFAFEYAKRSGRKKVTLVDKANVLTY--------AHDLWERVFAE-VSQEYDLETD 212
R +R A E+A R+G K VT++ K N++ Y A+DL F + V E ++ T
Sbjct: 207 QRHVRRAMEWAIRNGYKHVTIMHKGNIMKYTEGAFRQWAYDLILSEFRDYVVTEEEVNTK 266
Query: 213 H-------------YYVDAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAAS 259
+ D M ++I P ++V+VTPN+ GD ++D +VGG+G+AA
Sbjct: 267 YGGKAPEGKIIVNDRIADNMLQQIITRPGEYNVIVTPNLNGDYISDEANALVGGIGMAAG 326
Query: 260 GNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML-EYLNLEKESKWIEEAVKRA 318
++ + + EPVHGSAP AGK + NP A ILS +L +++ + +E AVK+A
Sbjct: 327 LDMG-DGIAVAEPVHGSAPKYAGKNVINPTAEILSGMYLLSDFVGWPEVKLLVEYAVKQA 385
Query: 319 IAENKVTPDM 328
IA +VT D+
Sbjct: 386 IAHKQVTYDL 395
>pir||T44658 isocitrate dehydrogenase (NADP+) (EC 1.1.1.42) [validated] -
Caldococcus noboribetus
Length = 433
Score = 148 bits (369), Expect = 1e-34
Identities = 120/370 (32%), Positives = 179/370 (47%), Gaps = 51/370 (13%)
Query: 3 LRIAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFE---FKEYPFGAEHYLKTGETLPDWA 59
L +A GDGIG E+V KVL + + + E G E + G LPD
Sbjct: 33 LIVAYFKGDGIGPEIVESAKKVLDAAVDKAYGGTRRIVWWEVTAGEEAQKECGSLLPDGT 92
Query: 60 IEEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN 119
++ FK G + P V G R + + +R LDLY N+RPVK Y + TP
Sbjct: 93 LQAFKLARVNLKGPLTTP-VGGGF--RSLNVTLRMVLDLYSNVRPVKWYG-QPTPHCHPE 148
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVE----------------- 162
ID V RENTE +YAG +P+ +++ + FG+E
Sbjct: 149 NIDWVIFRENTEDVYAGIEWPF--DSPEAQKIRDFLKKEFGIELTPDTGIGIKPISKWRT 206
Query: 163 -RVIRFAFEYAKRSGRKKVTLVDKANVLTY--------AHDLWERVFAE-VSQEYDLETD 212
R +R A E+A R+G K VT++ K N++ Y A+DL F + V E ++ T
Sbjct: 207 QRHVRRAMEWAIRNGYKHVTIMHKGNIMKYTEGAFRQWAYDLILSEFRDYVVTEEEVNTK 266
Query: 213 H-------------YYVDAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAAS 259
+ D M ++I P ++V+VTPN+ GD ++D +VGG+G+AA
Sbjct: 267 YGGKAPEGKIIVNDRIADNMLQQIITRPGEYNVIVTPNLNGDYISDEANPLVGGIGMAAG 326
Query: 260 GNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMML-EYLNLEKESKWIEEAVKRA 318
++ + + EPVHGSAP AGK + NP A ILS +L +++ + +E AVK+A
Sbjct: 327 LDMG-DGIAVAEPVHGSAPKYAGKNVINPTAEILSGMYLLSDFVGWPEVKLLVEYAVKQA 385
Query: 319 IAENKVTPDM 328
IA +VT D+
Sbjct: 386 IAHKQVTYDL 395
>sp|P39126|IDH_BACSU ISOCITRATE DEHYDROGENASE [NADP] (OXALOSUCCINATE DECARBOXYLASE)
(IDH) (NADP+-SPECIFIC ICDH) (IDP)
>gi|2117469|pir||I40382 isocitrate dehydrogenase (NADP+)
(EC 1.1.1.42) - Bacillus subtilis
>gi|487434|gb|AAA96342.1| (U05257) isocitrate
dehydrogenase [Bacillus subtilis]
>gi|2293268|gb|AAC00346.1| (AF008220) isocitrate
dehydrogenase [Bacillus subtilis]
>gi|2635378|emb|CAB14873.1| (Z99118) isocitrate
dehydrogenase [Bacillus subtilis]
Length = 423
Score = 146 bits (365), Expect = 3e-34
Identities = 127/413 (30%), Positives = 193/413 (45%), Gaps = 82/413 (19%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELS---RVSFEFKEYPFGAEHYLKTGETLPDWAIE 61
I I GDG G ++ KVL+ E + +KE G + Y KTGE LP ++
Sbjct: 22 IPFIEGDGTGPDIWNAASKVLEAAVEKAYKGEKKITWKEVYAGEKAYNKTGEWLPAETLD 81
Query: 62 EFKKFDAIYFGAIGDPRVKP---GILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGK 118
++ YF AI P P GI R + + +R ELDL+V LRPV+ + +P+K
Sbjct: 82 VIRE----YFIAIKGPLTTPVGGGI--RSLNVALRQELDLFVCLRPVRYFTGVPSPVKRP 135
Query: 119 NKIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMIN----------TRF--------- 159
D+V RENTE +YAG KG+ + VQ++I+ RF
Sbjct: 136 EDTDMVIFRENTEDIYAGIE--YAKGSEE---VQKLISFLQNELNVNKIRFPETSGIGIK 190
Query: 160 -----GVERVIRFAFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQ--------- 205
G R++R A +YA GRK VTLV K N++ + ++ E+++
Sbjct: 191 PVSEEGTSRLVRAAIDYAIEHGRKSVTLVHKGNIMKFTEGAFKNWGYELAEKEYGDKVFT 250
Query: 206 --EYDLETDHYYVDA-----------------------MAMKMIRSPESFDVVVTPNMFG 240
+YD + DA +++ P FDVV T N+ G
Sbjct: 251 WAQYDRIAEEQGKDAANKAQSEAEAAGKIIIKDSIADIFLQQILTRPNEFDVVATMNLNG 310
Query: 241 DILTDLGAEIVGGLGIAASGNINPET-TGMFEPVHGSAPDIAGKRIANPLAAILSASMML 299
D ++D A VGG+GIA NIN ET +FE HG+AP AG NP + ILS ++L
Sbjct: 311 DYISDALAAQVGGIGIAPGANINYETGHAIFEATHGTAPKYAGLDKVNPSSVILSGVLLL 370
Query: 300 EYLNLEKESKWIEEAVKRAIAENKVTPD----MNG--NLKTYEVGDWVVKFLE 346
E+L + + + +++++ IA VT D M+G +K E G+ ++K ++
Sbjct: 371 EHLGWNEAADLVIKSMEKTIASKVVTYDFARLMDGATEVKCSEFGEELIKNMD 423
>pir||T08743 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) beta chain -
human (fragment) >gi|4884118|emb|CAB43266.1| (AL050094)
hypothetical protein [Homo sapiens]
Length = 259
Score = 140 bits (349), Expect = 3e-32
Identities = 89/260 (34%), Positives = 142/260 (54%), Gaps = 9/260 (3%)
Query: 89 LLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDIVFVRENTEGLYAGAGGFLRKGTPQE 148
L+++R +LDL+ N+ VK +T N +D+V +RE TEG Y+ +G +
Sbjct: 3 LVRLRRKLDLFANVVHVKSLPGYMTR---HNNLDLVIIREQTEGEYSSLEHESARGVIEC 59
Query: 149 IAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYD 208
+ + TR +R+ +FAF+YA + GR KVT V KAN++ L+ + EV++ Y
Sbjct: 60 LKIV----TRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYP 115
Query: 209 -LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETT 267
++ + +D M+++++P FDV+V PN++G+I+ +L A +VGG G+ + + E
Sbjct: 116 KIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYA 175
Query: 268 GMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLNLEKESKWIEEAVKRAIAENKV-TP 326
G+ IANP A +LSAS ML +LNLE S I +AVK+ I KV T
Sbjct: 176 VFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTR 235
Query: 327 DMNGNLKTYEVGDWVVKFLE 346
DM G T + V+ L+
Sbjct: 236 DMGGYSTTTDFIKSVIGHLQ 255
>gi|4758582 isocitrate dehydrogenase 3 (NAD+) gamma [Homo sapiens]
>gi|1708404|sp|P51553|IDHG_HUMAN ISOCITRATE
DEHYDROGENASE [NAD] SUBUNIT GAMMA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|1167849|emb|CAA93143.1| (Z68907) NAD (H)-specific
isocitrate dehydrogenase gamma subunit precursor [Homo
sapiens] >gi|1302655|gb|AAC51744.1| (U52111)
NAD-isocitrate dehydrogenase [Homo sapiens]
>gi|1673432|emb|CAA92214.1| (Z68129) NAD(H)-specific
isocitrate dehydrogenase gamma-subunit precursor [Homo
sapiens] >gi|4096803|gb|AAD09357.1| (U40272)
NAD+-specific isocitrate dehydrogenase gamma subunit
precursor [Homo sapiens]
Length = 393
Score = 134 bits (335), Expect = 1e-30
Identities = 109/347 (31%), Positives = 170/347 (48%), Gaps = 20/347 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E++ +K + + V +F+E + E AI +
Sbjct: 57 VTMIPGDGIGPELMLH----VKSVFRHACVPVDFEEVHVSSN----ADEEDIRNAIMAIR 108
Query: 65 KFDAIYFGAIG-DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+ G I + + P R +L R LDLY N+ K +T K IDI
Sbjct: 109 RNRVALKGNIETNHNLPPSHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRHKD---IDI 163
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
+ VRENTEG Y+ L + + I T+ R+ +AF+ A+ SGRKKVT V
Sbjct: 164 LIVRENTEGEYSS----LEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAV 219
Query: 184 DKANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ L+ + EV+ Y T ++ VD M+++ P+ FDV+V PN++G+I
Sbjct: 220 HKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNI 279
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
+ ++ A +VGG G+ A N + IA K IANP A +L++ MML++L
Sbjct: 280 VNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHL 339
Query: 303 NLEKESKWIEEAVKRAI-AENKVTPDMNGNLKTYEVGDWVVKFLEVV 348
L + I +AV ++ EN TPD+ G T E V++ + V+
Sbjct: 340 KLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIRVI 386
>gi|11419927 isocitrate dehydrogenase 3 (NAD+) gamma [Homo sapiens]
Length = 556
Score = 134 bits (335), Expect = 1e-30
Identities = 109/347 (31%), Positives = 170/347 (48%), Gaps = 20/347 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E++ +K + + V +F+E + E AI +
Sbjct: 220 VTMIPGDGIGPELMLH----VKSVFRHACVPVDFEEVHVSSN----ADEEDIRNAIMAIR 271
Query: 65 KFDAIYFGAIG-DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+ G I + + P R +L R LDLY N+ K +T K IDI
Sbjct: 272 RNRVALKGNIETNHNLPPSHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRHKD---IDI 326
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
+ VRENTEG Y+ L + + I T+ R+ +AF+ A+ SGRKKVT V
Sbjct: 327 LIVRENTEGEYSS----LEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAV 382
Query: 184 DKANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ L+ + EV+ Y T ++ VD M+++ P+ FDV+V PN++G+I
Sbjct: 383 HKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNI 442
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
+ ++ A +VGG G+ A N + IA K IANP A +L++ MML++L
Sbjct: 443 VNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHL 502
Query: 303 NLEKESKWIEEAVKRAI-AENKVTPDMNGNLKTYEVGDWVVKFLEVV 348
L + I +AV ++ EN TPD+ G T E V++ + V+
Sbjct: 503 KLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIRVI 549
>sp|P41564|IDHG_MACFA ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT GAMMA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|631043|pir||S39065 isocitrate dehydrogenase (NAD+)
(EC 1.1.1.41) gamma chain precursor - crab-eating
macaque (fragment) >gi|415979|emb|CAA52224.1| (X74124)
NAD+-isocitrate dehydrogenase, gamma subunit [Macaca
fascicularis]
Length = 355
Score = 134 bits (334), Expect = 1e-30
Identities = 108/347 (31%), Positives = 170/347 (48%), Gaps = 20/347 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E++ +K + + V +F+E + E AI +
Sbjct: 19 VTMIPGDGIGPELMLH----VKSVFRHACVPVDFEEVHVSSN----ADEEDIRNAIMAIR 70
Query: 65 KFDAIYFGAIG-DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+ G I + + P R +L R LDLY N+ K +T K IDI
Sbjct: 71 RNRVALKGNIETNHNLPPSHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRHKD---IDI 125
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
+ VRENTEG Y+ L + + I T+ R+ +AF+ A+ SGRKKVT V
Sbjct: 126 LIVRENTEGEYSS----LEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAV 181
Query: 184 DKANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ L+ + EV+ Y T ++ VD M+++ P+ FDV+V PN++G+I
Sbjct: 182 HKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNI 241
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
+ ++ A +VGG G+ A N + IA K IANP A +L++ MML++L
Sbjct: 242 VNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHL 301
Query: 303 NLEKESKWIEEAVKRAI-AENKVTPDMNGNLKTYEVGDWVVKFLEVV 348
L + I +AV ++ EN TPD+ G T E +++ + V+
Sbjct: 302 KLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDIIRHIRVI 348
>gi|6680345 isocitrate dehydrogenase 3 (NAD+), gamma [Mus musculus]
>gi|2829481|sp|P70404|IDHG_MOUSE ISOCITRATE
DEHYDROGENASE [NAD] SUBUNIT GAMMA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
>gi|1546829|gb|AAC53340.1| (U68564) NAD(H)-specific
isocitrate dehydrogenase gamma subunit precursor [Mus
musculus] >gi|6651053|gb|AAF22149.1|AF133093_4
(AF133093) NAD(H)-specific isocitrate dehydrogenase
gamma subunit [Mus musculus]
Length = 393
Score = 133 bits (332), Expect = 3e-30
Identities = 107/347 (30%), Positives = 169/347 (47%), Gaps = 20/347 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E++ +K + + V +F+E + E AI +
Sbjct: 57 VTMIPGDGIGPELMLH----VKSVFRHACVPVDFEEVHVSSN----ADEEDIRNAIMAIR 108
Query: 65 KFDAIYFGAIG-DPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+ G I + + P R +L R LDLY N+ K +T K IDI
Sbjct: 109 RNRVALKGNIETNHNLPPSHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRHKD---IDI 163
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
+ VRENTEG Y+ L + + I T+ R+ +AF+ A+ SGRKKVT V
Sbjct: 164 LIVRENTEGEYSS----LEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAV 219
Query: 184 DKANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ L+ + EV+ Y T D VD M+++ P+ FDV+V PN++G+I
Sbjct: 220 HKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNI 279
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
+ ++ A +VGG G+ A N + IA K IANP A +L++ MML++L
Sbjct: 280 VNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHL 339
Query: 303 NLEKESKWIEEAVKRAI-AENKVTPDMNGNLKTYEVGDWVVKFLEVV 348
L + I +AV ++ EN TPD+ G T + +++ + ++
Sbjct: 340 KLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIRII 386
>emb|CAA52225.1| (X74125) NAD+-isocitrate dehydrogenase, gamma subunit [Rattus
norvegicus]
Length = 388
Score = 132 bits (330), Expect = 4e-30
Identities = 107/347 (30%), Positives = 168/347 (47%), Gaps = 20/347 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E++ +K + + V +F+E + E AI +
Sbjct: 52 VTMIPGDGIGPELMLH----VKSVFRHACVPVDFEEVHVSSN----ADEEDIRNAIMAIR 103
Query: 65 KFDAIYFGAIGDPR-VKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+ G I + P R +L R LDLY N+ K +T K IDI
Sbjct: 104 RNRVALKGNIETNHDLPPSHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRHKD---IDI 158
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
+ VRENTEG Y+ L + + I T+ R+ +AF+ A+ SGRKKVT V
Sbjct: 159 LIVRENTEGEYSS----LEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAV 214
Query: 184 DKANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ L+ + EV+ Y T D VD M+++ P+ FDV+V PN++G+I
Sbjct: 215 HKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNI 274
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
+ ++ A +VGG G+ A N + IA K IANP A +L++ MML++L
Sbjct: 275 VNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHL 334
Query: 303 NLEKESKWIEEAVKRAI-AENKVTPDMNGNLKTYEVGDWVVKFLEVV 348
L + I +AV ++ EN TPD+ G T + +++ + ++
Sbjct: 335 KLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIRII 381
>pir||S39064 isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) gamma chain precursor
- rat (fragment)
Length = 388
Score = 132 bits (330), Expect = 4e-30
Identities = 107/347 (30%), Positives = 168/347 (47%), Gaps = 20/347 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E++ +K + + V +F+E + E AI +
Sbjct: 52 VTMIPGDGIGPELMLH----VKSVFRHACVPVDFEEVHVSSN----ADEEDIRNAIMAIR 103
Query: 65 KFDAIYFGAIGDPR-VKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+ G I + P R +L R LDLY N+ K +T K IDI
Sbjct: 104 RNRVALKGNIETNHDLPPSHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRHKD---IDI 158
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
+ VRENTEG Y+ L + + I T+ R+ +AF+ A+ SGRKKVT V
Sbjct: 159 LIVRENTEGEYSS----LEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAV 214
Query: 184 DKANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ L+ + EV+ Y T D VD M+++ P+ FDV+V PN++G+I
Sbjct: 215 HKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNI 274
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
+ ++ A +VGG G+ A N + IA K IANP A +L++ MML++L
Sbjct: 275 VNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHL 334
Query: 303 NLEKESKWIEEAVKRAI-AENKVTPDMNGNLKTYEVGDWVVKFLEVV 348
L + I +AV ++ EN TPD+ G T + +++ + ++
Sbjct: 335 KLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIRII 381
>sp|P41565|IDHG_RAT ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT GAMMA, MITOCHONDRIAL
PRECURSOR (ISOCITRIC DEHYDROGENASE) (NAD+-SPECIFIC ICDH)
Length = 393
Score = 132 bits (330), Expect = 4e-30
Identities = 107/347 (30%), Positives = 168/347 (47%), Gaps = 20/347 (5%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEELSRVSFEFKEYPFGAEHYLKTGETLPDWAIEEFK 64
+ +IPGDGIG E++ +K + + V +F+E + E AI +
Sbjct: 57 VTMIPGDGIGPELMLH----VKSVFRHACVPVDFEEVHVSSN----ADEEDIRNAIMAIR 108
Query: 65 KFDAIYFGAIGDPR-VKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKNKIDI 123
+ G I + P R +L R LDLY N+ K +T K IDI
Sbjct: 109 RNRVALKGNIETNHDLPPSHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRHKD---IDI 163
Query: 124 VFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKKVTLV 183
+ VRENTEG Y+ L + + I T+ R+ +AF+ A+ SGRKKVT V
Sbjct: 164 LIVRENTEGEYSS----LEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAV 219
Query: 184 DKANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAMKMIRSPESFDVVVTPNMFGDI 242
KAN++ L+ + EV+ Y T D VD M+++ P+ FDV+V PN++G+I
Sbjct: 220 HKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNI 279
Query: 243 LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYL 302
+ ++ A +VGG G+ A N + IA K IANP A +L++ MML++L
Sbjct: 280 VNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHL 339
Query: 303 NLEKESKWIEEAVKRAI-AENKVTPDMNGNLKTYEVGDWVVKFLEVV 348
L + I +AV ++ EN TPD+ G T + +++ + ++
Sbjct: 340 KLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIRII 386
>gb|AAB39334.1| (U55996) isocitrate dehydrogenase [Thermus aquaticus]
Length = 426
Score = 132 bits (328), Expect = 8e-30
Identities = 108/347 (31%), Positives = 164/347 (47%), Gaps = 58/347 (16%)
Query: 56 PDWAIEEFKKFDAIYFGAIGDPRVKP---GILERGILLKMRFELDLYVNLRPVKLYHPRL 112
P W EE +F Y AI P P GI R I + +R ELDLY +RPV+ +
Sbjct: 82 PIWLPEETLEFIREYLVAIKGPLTTPVGGGI--RSINVALRQELDLYACVRPVRWFQGVP 139
Query: 113 TPLKGKNKIDIVFVRENTEGLYAG----AGG--------FLRKGTPQEIAVQEMINT--- 157
+P+K +++V RENTE +YAG AG FL++ P+ A T
Sbjct: 140 SPVKHPELVNMVIFRENTEDIYAGIEWPAGSEEVKKVLDFLKREFPKAYAKIRFPETSGL 199
Query: 158 ------RFGVERVIRFAFEYAKRSGRKKVTLVDKANVLTYAHDLW-ERVFAEVSQEYD-- 208
+ G ER++ A EYA + VTLV K N++ + + E +A ++Y
Sbjct: 200 GLKPISKEGTERLVEAAIEYAIKEDLPSVTLVHKGNIMKFTEGAFREWGYALAREKYGAT 259
Query: 209 -LETDHYYV-----------------DAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEI 250
L+ ++V D +++ P+ + V+ T N+ GD ++D A
Sbjct: 260 PLDGGPWHVLKNPRTGREIVIKDMIADNFLQQILLRPDEYSVIATMNLNGDYISDALAAQ 319
Query: 251 VGGLGIAASGNINPETT-GMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLNLEKESK 309
VGG+GIA NIN +T +FE HG+AP AG+ NP + ILS MML YL + +
Sbjct: 320 VGGIGIAPGANINYKTGHAVFEATHGTAPKYAGQDKVNPSSVILSGEMMLRYLGWNEAAD 379
Query: 310 WIEEAVKRAIAENKVTPDMN----------GNLKTYEVGDWVVKFLE 346
I A++R I++ VT D + LKT E G +++ ++
Sbjct: 380 LIIRAMERTISKGLVTYDFHRLLVAEGKPATLLKTSEFGQALIQHMD 426
>dbj|BAA91971.1| (AK001905) unnamed protein product [Homo sapiens]
Length = 233
Score = 120 bits (297), Expect = 3e-26
Identities = 72/194 (37%), Positives = 109/194 (56%), Gaps = 2/194 (1%)
Query: 155 INTRFGVERVIRFAFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYD-LETDH 213
I TR +R+ +FAF+YA + GR KVT V KAN++ L+ + EV++ Y ++ +
Sbjct: 36 IVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFET 95
Query: 214 YYVDAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPV 273
VD M+++++P FDV+V PN++G+I+ +L A +VGG G+ + + E
Sbjct: 96 MIVDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGA 155
Query: 274 HGSAPDIAGKRIANPLAAILSASMMLEYLNLEKESKWIEEAVKRAIAENKV-TPDMNGNL 332
G+ IANP A +LSAS ML +LNLE S I +AVK+ I KV T DM G
Sbjct: 156 RHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYS 215
Query: 333 KTYEVGDWVVKFLE 346
T + V+ L+
Sbjct: 216 TTTDFIKSVIGHLQ 229
>pir||T39871 3-isopropylmalate dehydrogenase - fission yeast
(Schizosaccharomyces pombe) >gi|2842527|emb|CAA16840.1|
(AL021746) 3-ISOPROPYLMALATE DEHYDROGENASE
[Schizosaccharomyces pombe]
Length = 220
Score = 118 bits (292), Expect = 1e-25
Identities = 78/206 (37%), Positives = 121/206 (57%), Gaps = 19/206 (9%)
Query: 161 VERVIRFAFEYAKRSGRKK-VTLVDKANVLTYAHDLWERVFAEV-SQEYD-LETDHYYVD 217
V R+ R A A+ S VTL+DKANVL + LW + A++ +EY L + +D
Sbjct: 15 VSRIARLAAWLAETSNPPAPVTLLDKANVLATSR-LWRKTVAKIFKEEYPHLTLKNQLID 73
Query: 218 AMAMKMIRSPESFD-VVVTPNMFGDILTDLGAEIVGGLGIAASGNIN-------PETTGM 269
+ AM +++SP + + VV+T N+FGDI++D + I G LG+ S +++ + +
Sbjct: 74 SAAMLLVKSPRTLNGVVLTDNLFGDIISDEASVIPGSLGLLPSASLSGVVGKSEEKVHCL 133
Query: 270 FEPVHGSAPDIAGKRIANPLAAILSASMMLEY-LNLEKESKWIEEAVKRAIAENKV---- 324
EP+HGSAPDIAGK I NP+ ILSAS++L Y LN KE++ IE AV++ + + +
Sbjct: 134 VEPIHGSAPDIAGKGIVNPVGTILSASLLLRYGLNAPKEAEAIEAAVRKVLDDTSIGGRG 193
Query: 325 --TPDMNGNLKTYEVGDWVVKFLEVV 348
T D+ G T ++ VV+ LE +
Sbjct: 194 LYTRDLGGEASTADITKAVVEELEKI 219
>gb|AAB70116.1| (U69269) NAD (H)-specific isocitrate dehydrogenase gamma subunit
[Homo sapiens]
Length = 210
Score = 106 bits (261), Expect = 5e-22
Identities = 66/200 (33%), Positives = 106/200 (53%), Gaps = 14/200 (7%)
Query: 163 RVIRFAFEYAKRSGRKKVTLVDKANVLTYAHDLWERVFAEVSQEYDLET-DHYYVDAMAM 221
R+ +AF+ A+ SGRKKVT V KAN++ L+ + EV+ Y T ++ VD M
Sbjct: 4 RIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTM 63
Query: 222 KMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIA 281
+++ P+ FDV+V PN++G+I+ ++ A +VGG G+ A N + IA
Sbjct: 64 QLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIA 123
Query: 282 GKRIANPLAAILSASMMLEYLNLEKESKWIEEAVKRAI-AENKV------------TPDM 328
K IANP A +L++ MML++L L + I +AV ++ EN + TPD+
Sbjct: 124 NKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENGLVCAEPACVLQMHTPDI 183
Query: 329 NGNLKTYEVGDWVVKFLEVV 348
G T E V++ + V+
Sbjct: 184 GGQGTTSEAIQDVIRHIRVI 203
>pir||T07167 probable isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) chain 1 -
tomato >gi|3687404|emb|CAA76076.1| (Y16126)
NADP-dependent isocitrate dehydrogenase-like protein
[Lycopersicon esculentum]
Length = 393
Score = 104 bits (258), Expect = 1e-21
Identities = 108/357 (30%), Positives = 169/357 (47%), Gaps = 37/357 (10%)
Query: 5 IAVIPGDGIGKEVVAEGLKVLKKLEE---LSR--VSFEFKEYPFGAEHYLKTGETLPDWA 59
+ +IPGDGIG V +V++ + L R V + KEY G + W
Sbjct: 59 VTLIPGDGIGPLVTGAVEQVMEAMHAPVLLWRYDVHGDMKEYASGDVDGISNPGRNKVWF 118
Query: 60 IEEFKKFDAIYFGAIGDPRVKPGILERGILLKMRFELDLYVNLRPVKLYHPRLTPLKGKN 119
E K V G+ + ++R ELDLY +L V ++ + P + +N
Sbjct: 119 KREGWKTP-----------VGGGVSSLNV--QLRKELDLYASL--VHCFNLKGLPTRHEN 163
Query: 120 KIDIVFVRENTEGLYAGAGGFLRKGTPQEIAVQEMINTRFGVERVIRFAFEYAKRSGRKK 179
+DIV +RENTEG Y+G + G + + V T+F ER+ ++AFEYA + RK
Sbjct: 164 -VDIVVIRENTEGEYSGLEHEVVPGVVESLKVM----TKFCSERIAKYAFEYAYLNNRKV 218
Query: 180 VTLVDKANVLTYAHDL--WERVFAEVSQ-EYDLETDHYYVDAMAMKMIRSPESFDVVVTP 236
VT V KAN++ + WE + Q T +A + +S + ++ +P
Sbjct: 219 VTAVHKANIMENLLMVYFWESCRRDRKQITLGSSTMRELWTTVACNLYQSLSNL-MLWSP 277
Query: 237 NMFGDI-LTDLGAEIVGGLGIAASGNINPETTGMFEPVHGSAPDIAGKRI-----ANPLA 290
+ +I LT+ A I GG G G +FE SA ++ ++I ANP+A
Sbjct: 278 LISTEIWLTNTAAGIAGGTGCLCPGGNVGGDHAVFEQ-GASAGNVGNEKILEQKKANPIA 336
Query: 291 AILSASMMLEYLNLEKESKWIEEAVKRAIAENK-VTPDMNGNLKTYEVGDWVVKFLE 346
LS++MML +L + +E +VKR IAE K +T D+ G+ T E+ D V+ L+
Sbjct: 337 LFLSSAMMLRHLQFPSFADRLETSVKRVIAEGKYMTKDLGGDCTTQEITDAVIANLD 393
>gb|AAF20164.1| (AF046929) NAD+-specific isocitrate dehydrogenase b subunit [Homo
sapiens]
Length = 156
Score = 86.2 bits (210), Expect = 5e-16
Identities = 52/147 (35%), Positives = 81/147 (54%), Gaps = 2/147 (1%)
Query: 202 EVSQEYD-LETDHYYVDAMAMKMIRSPESFDVVVTPNMFGDILTDLGAEIVGGLGIAASG 260
EV++ Y ++ + +D M+++++P FDV+V PN++G+I+ +L A +VGG G+
Sbjct: 6 EVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGE 65
Query: 261 NINPETTGMFEPVHGSAPDIAGKRIANPLAAILSASMMLEYLNLEKESKWIEEAVKRAIA 320
+ + E G+ IANP A +LSAS ML +LNLE S I +AVK+ I
Sbjct: 66 SYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIK 125
Query: 321 ENKV-TPDMNGNLKTYEVGDWVVKFLE 346
KV T DM G T + V+ L+
Sbjct: 126 VGKVRTRDMGGYSTTTDFIKSVIGHLQ 152
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.318 0.139 0.400
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 132049839
Number of Sequences: 2977
Number of extensions: 5586698
Number of successful extensions: 14016
Number of sequences better than 1.0e-10: 267
Number of HSP's better than 0.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 170
Number of HSP's that attempted gapping in prelim test: 12802
Number of HSP's gapped (non-prelim): 285
length of query: 354
length of database: 189,106,746
effective HSP length: 56
effective length of query: 298
effective length of database: 155,591,474
effective search space: 46366259252
effective search space used: 46366259252
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 165 (68.7 bits)