BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2438 (PAB2438) DE:LACTOYLGLUTATHIONE LYASE (EC 4.4.1.5)
(METHYLGLYOXALASE) (ALDOKETOMUTASE) (GLYOXALASE I).
(133 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||E75215 glyoxalase I related protein PAB2438 [imported] - Py... 264 3e-70
pir||A71452 glyoxalase I related protein PH0272 [similarity] - P... 249 1e-65
gi|11499800 conserved hypothetical protein [Archaeoglobus fulgid... 192 2e-48
dbj|BAB05187.1| (AP001512) unknown conserved protein [Bacillus h... 156 1e-37
gb|AAK42570.1| Lactoylglutathione lyase (glyoxalase I), putative... 120 8e-27
sp|P54540|YQJC_BACSU HYPOTHETICAL 15.7 KD PROTEIN IN GLNQ-ANSR I... 110 7e-24
emb|CAB70628.1| (AL137242) conserved hypothetical protein [Strep... 107 6e-23
pir||T20356 hypothetical protein D2030.5 - Caenorhabditis elegan... 102 2e-21
pir||B75324 conserved hypothetical protein - Deinococcus radiodu... 101 4e-21
pir||S72805 hypothetical protein B1549_F2_87 - Mycobacterium lep... 98 5e-20
gb|AAG19015.1| (AE005001) Vng0478c [Halobacterium sp. NRC-1] 91 5e-18
>pir||E75215 glyoxalase I related protein PAB2438 [imported] - Pyrococcus abyssi
(strain Orsay) >gi|5457681|emb|CAB49172.1| (AJ248283)
LACTOYLGLUTATHIONE LYASE (EC 4.4.1.5) METHYLGLYOXALASE)
(ALDOKETOMUTASE) (GLYOXALASE I). [Pyrococcus abyssi]
Length = 133
Score = 264 bits (668), Expect = 3e-70
Identities = 133/133 (100%), Positives = 133/133 (100%)
Query: 1 MFKKIDHVGIAVKNLEEAIKVWEGLGFKVEEVEEVPDQKVKVAVIKVGESRIELLEATSE 60
MFKKIDHVGIAVKNLEEAIKVWEGLGFKVEEVEEVPDQKVKVAVIKVGESRIELLEATSE
Sbjct: 1 MFKKIDHVGIAVKNLEEAIKVWEGLGFKVEEVEEVPDQKVKVAVIKVGESRIELLEATSE 60
Query: 61 DSPIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKS 120
DSPIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKS
Sbjct: 61 DSPIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKS 120
Query: 121 VTGVLLELCERKE 133
VTGVLLELCERKE
Sbjct: 121 VTGVLLELCERKE 133
>pir||A71452 glyoxalase I related protein PH0272 [similarity] - Pyrococcus
horikoshii >gi|3256661|dbj|BAA29344.1| (AP000001) 136aa
long hypothetical protein [Pyrococcus horikoshii]
Length = 136
Score = 249 bits (628), Expect = 1e-65
Identities = 120/133 (90%), Positives = 131/133 (98%)
Query: 1 MFKKIDHVGIAVKNLEEAIKVWEGLGFKVEEVEEVPDQKVKVAVIKVGESRIELLEATSE 60
MFK+IDHVGIAVKNLEEAIK+WEGLGFKVEE+EEVPDQKVKVAVIKVGE+RIELLEAT+E
Sbjct: 4 MFKRIDHVGIAVKNLEEAIKIWEGLGFKVEEIEEVPDQKVKVAVIKVGENRIELLEATTE 63
Query: 61 DSPIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKS 120
DSPIAKFIEKRGEGIHHLAI VENIE KL+ELK+KGY+LIDE+PR+GAGGAKIAF+HPKS
Sbjct: 64 DSPIAKFIEKRGEGIHHLAIRVENIESKLEELKQKGYKLIDEKPRVGAGGAKIAFIHPKS 123
Query: 121 VTGVLLELCERKE 133
VTGVLLELCERKE
Sbjct: 124 VTGVLLELCERKE 136
>gi|11499800 conserved hypothetical protein [Archaeoglobus fulgidus]
>gi|7448628|pir||B69527 conserved hypothetical protein
AF2218 - Archaeoglobus fulgidus
>gi|2648311|gb|AAB89040.1| (AE000952) conserved
hypothetical protein [Archaeoglobus fulgidus]
Length = 131
Score = 192 bits (482), Expect = 2e-48
Identities = 92/130 (70%), Positives = 109/130 (83%)
Query: 1 MFKKIDHVGIAVKNLEEAIKVWEGLGFKVEEVEEVPDQKVKVAVIKVGESRIELLEATSE 60
M +KIDHVG+AVK+LEEA+K +E LGF VEE+EEV +QKV+VA++ GESR+ELLEATSE
Sbjct: 1 MIRKIDHVGVAVKSLEEAVKTYEALGFGVEEIEEVAEQKVRVAMLPAGESRVELLEATSE 60
Query: 61 DSPIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKS 120
DS IAKFI RGEGIHH+A+ VENIEE L + KE G RLIDE+PR+GAGG K+AFVHPKS
Sbjct: 61 DSAIAKFISSRGEGIHHIAVNVENIEEALNKAKEAGLRLIDEKPRVGAGGKKVAFVHPKS 120
Query: 121 VTGVLLELCE 130
GVLLE E
Sbjct: 121 THGVLLEFVE 130
>dbj|BAB05187.1| (AP001512) unknown conserved protein [Bacillus halodurans]
Length = 138
Score = 156 bits (390), Expect = 1e-37
Identities = 72/131 (54%), Positives = 107/131 (80%), Gaps = 1/131 (0%)
Query: 4 KIDHVGIAVKNLEEAIKVWEG-LGFKVEEVEEVPDQKVKVAVIKVGESRIELLEATSEDS 62
K+DH+GIAV ++++ + + G L K+ +E++P Q VK+A +++GES+IELLE SE+S
Sbjct: 7 KLDHIGIAVTSIKDVLPFYVGSLKLKLLGMEDLPSQGVKIAFLEIGESKIELLEPLSEES 66
Query: 63 PIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKSVT 122
PIAKFI+KRGEGIHH+AIGV++IEE+++E+KE G ++I++EP GA GA++AF+HP+S
Sbjct: 67 PIAKFIQKRGEGIHHIAIGVKSIEERIQEVKENGVQMINDEPVPGARGAQVAFLHPRSAR 126
Query: 123 GVLLELCERKE 133
GVL E CE+KE
Sbjct: 127 GVLYEFCEKKE 137
>gb|AAK42570.1| Lactoylglutathione lyase (glyoxalase I), putative [Sulfolobus
solfataricus]
Length = 142
Score = 120 bits (297), Expect = 8e-27
Identities = 64/138 (46%), Positives = 92/138 (66%), Gaps = 7/138 (5%)
Query: 3 KKIDHVGIAVKNLEEAIKVWEG-LGFKVEEVEEVPDQKVKVAVI---KVGESRIELLEAT 58
+ +DH+GI V NL+EAIK ++ G K+ E +P++ +KVA + + E+ IELLE
Sbjct: 4 ENLDHIGIVVGNLDEAIKFYQNTFGMKLVHYELIPERGIKVAFLVSNEKNETSIELLEPI 63
Query: 59 SED---SPIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAF 115
+ + +AKF++ RG+G+HHLAI V +I L+EL KG +L+D PR GA G +AF
Sbjct: 64 DHNDMSNTVAKFLKNRGQGLHHLAIKVSDINRALEELTAKGLQLVDTSPRKGARGHLVAF 123
Query: 116 VHPKSVTGVLLELCERKE 133
+HPKSV GVLLEL + KE
Sbjct: 124 LHPKSVMGVLLELVQAKE 141
>sp|P54540|YQJC_BACSU HYPOTHETICAL 15.7 KD PROTEIN IN GLNQ-ANSR INTERGENIC REGION
>gi|7448630|pir||C69963 conserved hypothetical protein
yqjC - Bacillus subtilis >gi|1303954|dbj|BAA12609.1|
(D84432) YqjC [Bacillus subtilis]
>gi|2634827|emb|CAB14324.1| (Z99116) similar to
hypothetical proteins [Bacillus subtilis]
Length = 140
Score = 110 bits (272), Expect = 7e-24
Identities = 55/130 (42%), Positives = 83/130 (63%), Gaps = 1/130 (0%)
Query: 4 KIDHVGIAVKNLEEAIKVWEG-LGFKVEEVEEVPDQKVKVAVIKVGESRIELLEATSEDS 62
++DH+GIAV +++A +E LG E V +QKV VA + G ++ L+E + DS
Sbjct: 3 RLDHIGIAVFPIKDARSFYENVLGLAFLHQETVEEQKVNVAFFQAGSVKLGLIEPLTADS 62
Query: 63 PIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKSVT 122
P+ F+EK+G+G+HH+A + E+L+ L ++ +LID PR GA G KIAF+ P+
Sbjct: 63 PVHLFLEKKGQGLHHIAFLCNCLSEQLQALSDQHVQLIDRFPRQGANGKKIAFISPRETN 122
Query: 123 GVLLELCERK 132
GVL+ELCE K
Sbjct: 123 GVLVELCEPK 132
>emb|CAB70628.1| (AL137242) conserved hypothetical protein [Streptomyces coelicolor
A3(2)]
Length = 146
Score = 107 bits (264), Expect = 6e-23
Identities = 52/135 (38%), Positives = 86/135 (63%), Gaps = 7/135 (5%)
Query: 1 MFKKIDHVGIAVKNLEEAIKVWEGL-GFKVEEVEEVPDQKVKVAVIKV------GESRIE 53
M +IDH+GIA +L+ ++ + GF+V E +Q V+ A++K+ G S ++
Sbjct: 1 MLTRIDHIGIACHDLDATVEFYRATYGFEVFHTEVNEEQGVREAMLKINDTSDGGASYLQ 60
Query: 54 LLEATSEDSPIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKI 113
LLE T EDS + K++ K GEG+HH+A G +++ ++++KG R++ +EPR G+ G++I
Sbjct: 61 LLEPTREDSAVGKWLAKNGEGVHHIAFGTADVDADAADIRDKGVRVLYDEPRRGSMGSRI 120
Query: 114 AFVHPKSVTGVLLEL 128
F+HPK GVL EL
Sbjct: 121 TFLHPKDCHGVLTEL 135
>pir||T20356 hypothetical protein D2030.5 - Caenorhabditis elegans
>gi|3875398|emb|CAA98118.1| (Z73906) Similarity to
B.subtilis YQJC protein (TR:G1303954), contains
similarity to Pfam domain: PF01626
(4-hydroxyphenylpyruvate dioxygenase C terminal domain),
Score=157.2, E-value=8.9e-44, N=1~cDNA EST EMBL:T01187
comes from this gene [Caenorhabdit>
Length = 162
Score = 102 bits (252), Expect = 2e-21
Identities = 51/126 (40%), Positives = 79/126 (62%), Gaps = 1/126 (0%)
Query: 4 KIDHVGIAVKNLEEAIKVWEGLGFKVEEVEEVPDQKVKVAVIKVGESRIELLEATSEDSP 63
K++HV IA +L+++ + ++GLG KV E P+ V +++ S+IELL E SP
Sbjct: 34 KLNHVAIATPDLKKSSEFYKGLGAKVSEAVPQPEHGVYTVFVELPNSKIELLHPFGEKSP 93
Query: 64 IAKFIEKRGEG-IHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKSVT 122
I F+ K +G +HH+ I V +I E + +K KG R + E+P+IGA G ++ F+HPK
Sbjct: 94 IQAFLNKNKDGGMHHICIEVRDIHEAVSAVKTKGIRTLGEKPKIGAHGKEVMFLHPKDCG 153
Query: 123 GVLLEL 128
GVL+EL
Sbjct: 154 GVLIEL 159
>pir||B75324 conserved hypothetical protein - Deinococcus radiodurans (strain
R1) >gi|6459812|gb|AAF11572.1|AE002039_2 (AE002039)
conserved hypothetical protein [Deinococcus radiodurans]
Length = 154
Score = 101 bits (249), Expect = 4e-21
Identities = 52/127 (40%), Positives = 78/127 (60%), Gaps = 1/127 (0%)
Query: 5 IDHVGIAVKNLEEAIKVWEGLGFKVEEVEE-VPDQKVKVAVIKVGESRIELLEATSEDSP 63
+DHV IA +L++ + LG + E +E V Q V+V VG++ IELL T DSP
Sbjct: 3 LDHVAIATPDLDQGAAPYVALGLRPEGPDEDVGTQGVRVRAFVVGDTLIELLMPTRPDSP 62
Query: 64 IAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKSVTG 123
IA F+EK+G G+HH A VE+++ +++ L+ +G R + E P G G ++ F+HPK G
Sbjct: 63 IAGFLEKKGPGLHHTAYRVEHLDAEIERLRGEGARFLSETPGPGRAGTRVVFLHPKWGQG 122
Query: 124 VLLELCE 130
L+EL E
Sbjct: 123 TLIELVE 129
>pir||S72805 hypothetical protein B1549_F2_87 - Mycobacterium leprae
>gi|466927|gb|AAA50902.1| (U00014) B1549_F2_87
[Mycobacterium leprae]
Length = 155
Score = 97.5 bits (239), Expect = 5e-20
Identities = 54/132 (40%), Positives = 80/132 (59%), Gaps = 6/132 (4%)
Query: 5 IDHVGIAVKNLEEAIKVW-EGLGFKVEEVEEVPDQKVKVAVIK-----VGESRIELLEAT 58
IDHVGIAV +L AI + + LG + E DQ ++ A++ +G ++I+L+
Sbjct: 21 IDHVGIAVADLNAAISWYHDHLGMILVHEEVNDDQGIREAMLSAHGAPIGTTQIQLMAPI 80
Query: 59 SEDSPIAKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHP 118
+E SPIAKF++K G GI LA V +++ K L+ +G RL+ + PR G A+I F+HP
Sbjct: 81 NESSPIAKFLDKHGPGIQQLACRVSDLDRLTKRLRAQGVRLVYDTPRQGTSNARINFIHP 140
Query: 119 KSVTGVLLELCE 130
K GVL+EL E
Sbjct: 141 KDAGGVLIELVE 152
>gb|AAG19015.1| (AE005001) Vng0478c [Halobacterium sp. NRC-1]
Length = 138
Score = 90.9 bits (222), Expect = 5e-18
Identities = 46/126 (36%), Positives = 73/126 (57%)
Query: 5 IDHVGIAVKNLEEAIKVWEGLGFKVEEVEEVPDQKVKVAVIKVGESRIELLEATSEDSPI 64
+DHVG+A ++ + +++ L EEV D V + G+ +ELLE ED+ +
Sbjct: 12 VDHVGVATEDAADLGELYTALLGAPVAHEEVFDGLHTVFLDLGGDGYVELLEPVDEDTTV 71
Query: 65 AKFIEKRGEGIHHLAIGVENIEEKLKELKEKGYRLIDEEPRIGAGGAKIAFVHPKSVTGV 124
+++++ G GIHH+A ++IE L+ ++ G IDEEPR GA G +AF+HPK G
Sbjct: 72 GRYLQRTGGGIHHVAFATDDIEAALETARDAGVDRIDEEPRAGAWGHDVAFLHPKDTGGA 131
Query: 125 LLELCE 130
L+E E
Sbjct: 132 LIEFVE 137
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.314 0.138 0.377
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49063043
Number of Sequences: 2977
Number of extensions: 1919761
Number of successful extensions: 6164
Number of sequences better than 1.0e-10: 11
Number of HSP's better than 0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 6141
Number of HSP's gapped (non-prelim): 11
length of query: 133
length of database: 189,106,746
effective HSP length: 56
effective length of query: 77
effective length of database: 155,591,474
effective search space: 11980543498
effective search space used: 11980543498
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 42 (21.9 bits)
S2: 160 (66.7 bits)