BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2441 (aif1A) DE:translation initiation factor aIF-1A (aif1A)
(115 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||B75179 translation initiation factor aif-1a (aif1a) PAB2441... 241 2e-63
pir||H71034 probable translation initiation factor eIF-1A - Pyro... 239 1e-62
sp|Q57887|IF1A_METJA PUTATIVE TRANSLATION INITIATION FACTOR 1A (... 115 3e-25
sp|O29481|IF1A_ARCFU PROBABLE TRANSLATION INITIATION FACTOR 1A (... 109 2e-23
sp|O27085|IF1A_METTH PUTATIVE TRANSLATION INITIATION FACTOR 1A (... 106 8e-23
gi|11498383 translation initiation factor eIF-1A (eif1A) [Archae... 103 7e-22
sp|Q03590|IF1A_THEAC PROBABLE TRANSLATION INITIATION FACTOR 1A (... 90 8e-18
pir||S26726 hypothetical protein 125 (rpoA2 3' region) - Thermop... 87 9e-17
sp|P57676|IF1A_AERPE PROBABLE TRANSLATION INITIATION FACTOR 1A (... 83 1e-15
gb|AAG20357.1| (AE005109) translation initiation factor eIF-1A; ... 82 3e-15
gb|AAK42526.1| Translation initiation factor 1A homolog (EIF 1A)... 78 3e-14
gb|AAC69495.1| (AF099920) Contains similarity to Pfam domain: PF... 71 6e-12
gb|AAG19983.1| (AE005080) translation initiation factor eIF-1A; ... 71 6e-12
>pir||B75179 translation initiation factor aif-1a (aif1a) PAB2441 - Pyrococcus
abyssi (strain Orsay) >gi|5458023|emb|CAB49513.1|
(AJ248284) translation initiation factor aIF-1A (aif1A)
[Pyrococcus abyssi]
Length = 115
Score = 241 bits (609), Expect = 2e-63
Identities = 115/115 (100%), Positives = 115/115 (100%)
Query: 1 MLMPKKERKVEGDEVIRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRR 60
MLMPKKERKVEGDEVIRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRR
Sbjct: 1 MLMPKKERKVEGDEVIRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRR 60
Query: 61 VWIRVGDLVIVQPWPVQSDKRGDIVYRYTQTQVDWLLRKGKITQEFLTGGSLLVE 115
VWIRVGDLVIVQPWPVQSDKRGDIVYRYTQTQVDWLLRKGKITQEFLTGGSLLVE
Sbjct: 61 VWIRVGDLVIVQPWPVQSDKRGDIVYRYTQTQVDWLLRKGKITQEFLTGGSLLVE 115
>pir||H71034 probable translation initiation factor eIF-1A - Pyrococcus
horikoshii >gi|3257997|dbj|BAA30680.1| (AP000006) 116aa
long hypothetical translation initiation factor eIF-1A
[Pyrococcus horikoshii]
Length = 116
Score = 239 bits (603), Expect = 1e-62
Identities = 113/115 (98%), Positives = 115/115 (99%)
Query: 1 MLMPKKERKVEGDEVIRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRR 60
M+MPKKERKVEG+EVIRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRR
Sbjct: 2 MIMPKKERKVEGEEVIRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRR 61
Query: 61 VWIRVGDLVIVQPWPVQSDKRGDIVYRYTQTQVDWLLRKGKITQEFLTGGSLLVE 115
VWIRVGDLVIVQPWPVQSDKRGDIVYRYTQTQVDWLLRKGKITQEFLTGGSLLVE
Sbjct: 62 VWIRVGDLVIVQPWPVQSDKRGDIVYRYTQTQVDWLLRKGKITQEFLTGGSLLVE 116
>sp|Q57887|IF1A_METJA PUTATIVE TRANSLATION INITIATION FACTOR 1A (EIF-1A)
>gi|2129322|pir||E64355 translation initiation factor
aIF-1A - Methanococcus jannaschii
>gi|1592291|gb|AAB98435.1| (U67496) translation
initiation factor aIF-1A [Methanococcus jannaschii]
Length = 102
Score = 115 bits (284), Expect = 3e-25
Identities = 50/98 (51%), Positives = 73/98 (74%)
Query: 7 ERKVEGDEVIRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWIRVG 66
E++ E +R+P E N++ G++EQ LGA + VRC DGK R RIPG+L+ R+W+R G
Sbjct: 3 EQQQEQQIRVRIPRKEENEILGIIEQMLGASRVRVRCLDGKTRLGRIPGRLKNRIWVREG 62
Query: 67 DLVIVQPWPVQSDKRGDIVYRYTQTQVDWLLRKGKITQ 104
D+VIV+PW VQ D++ DI++RYT+TQV+WL RKG + +
Sbjct: 63 DVVIVKPWEVQGDQKCDIIWRYTKTQVEWLKRKGYLDE 100
>sp|O29481|IF1A_ARCFU PROBABLE TRANSLATION INITIATION FACTOR 1A (EIF-1A)
Length = 97
Score = 109 bits (269), Expect = 2e-23
Identities = 55/90 (61%), Positives = 66/90 (73%), Gaps = 3/90 (3%)
Query: 13 DEVIRVPLPEGN--QLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWIRVGDLVI 70
D+VIRV LP+ +LFGVV LGAG + VRCEDG R RIPGK+R+++WIR GD+VI
Sbjct: 5 DDVIRVRLPDRKKGELFGVVTSMLGAGHIKVRCEDGVERLARIPGKMRKKIWIREGDVVI 64
Query: 71 VQPWPVQSDKRGDIVYRYTQTQVDWLLRKG 100
V PW Q D R DIV+RYT QV+WL RKG
Sbjct: 65 VVPWSFQKD-RADIVWRYTNPQVEWLERKG 93
>sp|O27085|IF1A_METTH PUTATIVE TRANSLATION INITIATION FACTOR 1A (EIF-1A)
>gi|7447625|pir||H69000 translation initiation factor
aIF-1A - Methanobacterium thermoautotrophicum (strain
Delta H) >gi|2622105|gb|AAB85500.1| (AE000873)
translation initiation factor, eIF-1A [Methanobacterium
thermoautotrophicum]
Length = 99
Score = 106 bits (263), Expect = 8e-23
Identities = 48/87 (55%), Positives = 66/87 (75%)
Query: 14 EVIRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWIRVGDLVIVQP 73
EV RV P ++ GVVEQ +G G + VRC DG IR RIPGK+++R+WIR GD+V+V+P
Sbjct: 9 EVRRVRTPRRGEIPGVVEQIMGHGKLKVRCADGHIRLGRIPGKMKKRIWIREGDVVLVKP 68
Query: 74 WPVQSDKRGDIVYRYTQTQVDWLLRKG 100
W QS+++ DIV+RYT+T+ +WL RKG
Sbjct: 69 WEFQSEEKADIVWRYTRTESNWLERKG 95
>gi|11498383 translation initiation factor eIF-1A (eif1A) [Archaeoglobus
fulgidus] >gi|7447624|pir||A69347 translation initiation
factor eIF-1A (eif1A) homolog - Archaeoglobus fulgidus
>gi|2649837|gb|AAB90469.1| (AE001051) translation
initiation factor eIF-1A (eif1A) [Archaeoglobus
fulgidus]
Length = 88
Score = 103 bits (255), Expect = 7e-22
Identities = 50/85 (58%), Positives = 62/85 (72%), Gaps = 1/85 (1%)
Query: 16 IRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWIRVGDLVIVQPWP 75
+R+P + +LFGVV LGAG + VRCEDG R RIPGK+R+++WIR GD+VIV PW
Sbjct: 1 MRLPDRKKGELFGVVTSMLGAGHIKVRCEDGVERLARIPGKMRKKIWIREGDVVIVVPWS 60
Query: 76 VQSDKRGDIVYRYTQTQVDWLLRKG 100
Q D R DIV+RYT QV+WL RKG
Sbjct: 61 FQKD-RADIVWRYTNPQVEWLERKG 84
>sp|Q03590|IF1A_THEAC PROBABLE TRANSLATION INITIATION FACTOR 1A (EIF-1A)
Length = 119
Score = 90.1 bits (220), Expect = 8e-18
Identities = 47/91 (51%), Positives = 63/91 (68%), Gaps = 4/91 (4%)
Query: 11 EGDEVI-RVPLP--EGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWIRVGD 67
E +E I RV LP + ++FG+VE+ GA + V CEDG R RIPG++R+R+WIR D
Sbjct: 18 ENEESIGRVILPNKKKGEMFGIVEKMEGASRLSVMCEDGYTRNARIPGRMRKRMWIREKD 77
Query: 68 LVIVQPWPVQSDKRGDIVYRYTQTQVDWLLR 98
LVIV+PW Q +K D+VYRYT+TQ +L R
Sbjct: 78 LVIVKPWEFQPEK-ADVVYRYTKTQASYLSR 107
>pir||S26726 hypothetical protein 125 (rpoA2 3' region) - Thermoplasma
acidophilum >gi|48095|emb|CAA48284.1| (X68198) ORF125
[Thermoplasma acidophilum]
Length = 125
Score = 86.6 bits (211), Expect = 9e-17
Identities = 46/91 (50%), Positives = 62/91 (67%), Gaps = 4/91 (4%)
Query: 11 EGDEVI-RVPLP--EGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWIRVGD 67
E +E I RV LP + ++FG+VE+ GA + V CEDG R RIPG++R+R+WIR D
Sbjct: 18 ENEESIGRVILPNKKKGEMFGIVEKMEGASRLSVMCEDGYTRNARIPGRMRKRMWIREKD 77
Query: 68 LVIVQPWPVQSDKRGDIVYRYTQTQVDWLLR 98
LVIV+PW Q +K D+VYR T+TQ +L R
Sbjct: 78 LVIVKPWEFQPEK-ADVVYRNTKTQASYLSR 107
>sp|P57676|IF1A_AERPE PROBABLE TRANSLATION INITIATION FACTOR 1A (EIF-1A)
Length = 111
Score = 83.1 bits (202), Expect = 1e-15
Identities = 37/92 (40%), Positives = 64/92 (69%), Gaps = 3/92 (3%)
Query: 18 VPLPEGNQ--LFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWIRVGDLVIVQPWP 75
+PLP ++ + +V++ +GAG+++V C DG++ RIPGK+RRRVW+R GD+V+ PW
Sbjct: 14 MPLPSEDEGTMLCIVQRVVGAGFLEVLCTDGEVYMARIPGKMRRRVWMREGDVVLFLPWG 73
Query: 76 VQSDKRGDIVYRYTQTQVDWLLRKGKITQEFL 107
+DK+G++VYRY + +V L+ + +E +
Sbjct: 74 T-ADKKGEVVYRYLRDEVRKLIDMNLLPEELV 104
>gb|AAG20357.1| (AE005109) translation initiation factor eIF-1A; Eif1a1
[Halobacterium sp. NRC-1]
Length = 95
Score = 81.5 bits (198), Expect = 3e-15
Identities = 36/83 (43%), Positives = 55/83 (65%), Gaps = 1/83 (1%)
Query: 20 LPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWIRVGDLVIVQPWPVQSD 79
+PE +++F V LGA + VRC DG+ R RIPG++++R+WIR D+V+V+PW Q D
Sbjct: 13 MPEDDEVFAEVTDMLGANRVQVRCADGEERTARIPGRMQKRIWIREDDIVLVEPWDWQDD 72
Query: 80 KRGDIVYRYTQTQVDWLLRKGKI 102
K D+ +RY ++ D L +G I
Sbjct: 73 K-ADVTWRYEKSDADQLREEGHI 94
>gb|AAK42526.1| Translation initiation factor 1A homolog (EIF 1A) (eif1A) (eiF1A)
[Sulfolobus solfataricus]
Length = 108
Score = 78.4 bits (190), Expect = 3e-14
Identities = 43/105 (40%), Positives = 64/105 (60%), Gaps = 2/105 (1%)
Query: 3 MPKKERKVEGDEVIRVPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVW 62
MPKK+R E VP PE Q VV++ LG + V C DGK R RIPGK+R+++W
Sbjct: 1 MPKKDRAQEAPSR-DVPRPEEGQTICVVKKMLGGDHLIVLCMDGKERLARIPGKIRKKMW 59
Query: 63 IRVGDLVIVQPWPVQSDKRGDIVYRYTQTQVDWLLRKGKITQEFL 107
+R GD+V+V W Q + R DI+Y+Y ++ L+ + I++E +
Sbjct: 60 MREGDVVLVGIWDFQPN-RCDILYKYGNDEIKRLVNENIISREVI 103
>gb|AAC69495.1| (AF099920) Contains similarity to Pfam domain: PF01176 (eIF-1a),
Score=207.8, E-value=5.4e-59, N=1 [Caenorhabditis
elegans]
Length = 216
Score = 70.6 bits (170), Expect = 6e-12
Identities = 41/101 (40%), Positives = 59/101 (57%), Gaps = 2/101 (1%)
Query: 5 KKERKVEGDEVIR-VPLPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWI 63
++ K E D + R + L E Q +G V + LG G + V C DGK R C I GKLR++VWI
Sbjct: 12 RRRGKNENDFMKRELDLKEEGQEYGQVSKMLGNGRVQVFCFDGKQRVCHIRGKLRKKVWI 71
Query: 64 RVGDLVIVQPWPVQSDKRGDIVYRYTQTQVDWLLRKGKITQ 104
VGD+++V Q DK GD++ +YT + L +G I +
Sbjct: 72 NVGDIILVGLRDYQDDK-GDVILKYTPDEARRLKNEGLIPE 111
>gb|AAG19983.1| (AE005080) translation initiation factor eIF-1A; Eif1a2
[Halobacterium sp. NRC-1]
Length = 83
Score = 70.6 bits (170), Expect = 6e-12
Identities = 31/83 (37%), Positives = 53/83 (63%), Gaps = 1/83 (1%)
Query: 20 LPEGNQLFGVVEQALGAGWMDVRCEDGKIRRCRIPGKLRRRVWIRVGDLVIVQPWPVQSD 79
+P +++F VV++ G + ++CEDGK R RIPG+++ R WI GD+V+ +PW Q D
Sbjct: 1 MPNDDEVFAVVKRHDGGNHVTLQCEDGKERMGRIPGRMKYRTWINEGDVVLAEPWDWQ-D 59
Query: 80 KRGDIVYRYTQTQVDWLLRKGKI 102
++ ++ +RY+ D L +G I
Sbjct: 60 EKANVEWRYSDQDADQLREEGHI 82
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.323 0.144 0.453
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46239959
Number of Sequences: 2977
Number of extensions: 1825077
Number of successful extensions: 2931
Number of sequences better than 1.0e-10: 13
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2915
Number of HSP's gapped (non-prelim): 13
length of query: 115
length of database: 189,106,746
effective HSP length: 46
effective length of query: 69
effective length of database: 161,576,344
effective search space: 11148767736
effective search space used: 11148767736
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.9 bits)
S2: 160 (66.7 bits)