BLASTP 2.0.10 [Aug-26-1999]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PAB2444 (aIF-2BI) DE:translation initiation factor aIF-2BI
(356 letters)
Database: ./suso.pep; /banques/blast2/nr.pep
598,487 sequences; 189,106,746 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UZ16|E2B1_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 716 0.0
sp|O58433|E2B1_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 658 0.0
sp|Q9X013|E2B_THEMA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 328 5e-89
sp|O67879|E2B_AQUAE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 309 4e-83
sp|P74497|E2B_SYNY3 PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 286 3e-76
pir||A83249 probable initiation factor 2 subunit PA3169 [importe... 285 6e-76
sp|O31662|E2B_BACSU PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 281 7e-75
gi|11497982 translation initiation factor eIF-2B, subunit delta ... 278 6e-74
sp|Q57896|E2B1_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 276 2e-73
emb|CAC12337.1| (AL445066) translation initiation factor aIF-2B,... 272 5e-72
gb|AAK43082.1| Translation initiation factor aiF-2B, subunit 1 (... 266 2e-70
sp|O27900|E2B1_METTH PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 261 1e-68
sp|Q9PAG5|E2B_XYLFA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2... 259 4e-68
sp|Q9YE84|E2B1_AERPE PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 251 1e-65
gb|AAC95160.1| (AC005970) putative translation initiation factor... 244 1e-63
pir||T39944 hypothetical translation initiation factor - fission... 220 2e-56
gb|AAF57154.1| (AE003778) CG11334 gene product [alt 1] [Drosophi... 216 4e-55
emb|CAB90925.1| (AL355774) putative translation initiation facto... 213 2e-54
sp|Q93169|YQJQ_CAEEL HYPOTHETICAL 40.9 KD PROTEIN C01G10.9 IN CH... 207 2e-52
gi|6325375 Ypr118wp [Saccharomyces cerevisiae] >gi|3183489|sp|Q0... 180 2e-44
sp|Q57586|E2B2_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 154 1e-36
sp|O57947|E2B2_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 151 1e-35
sp|Q9V281|E2B2_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 147 2e-34
gi|11499619 translation initiation factor eIF-2B, subunit delta ... 144 1e-33
gb|AAG20059.1| (AE005085) translation initiation factor eIF-2B s... 131 1e-29
emb|CAA39062.1| (X55387) ORF3 [Rhodospirillum rubrum] 99 7e-20
sp|P23140|YPE3_RHORU HYPOTHETICAL PROTEIN IN PETC 3'REGION >gi|7... 99 7e-20
pir||T40858 translation initiation factor eif-2b alpha subunit -... 87 2e-16
sp|O58185|E2BL_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 87 4e-16
gb|AAF27156.1|AC016529_19 (AC016529) putative translation initia... 76 4e-13
gb|AAF02860.1|AC009324_9 (AC009324) Similar to initiation factor... 74 3e-12
sp|Q9UYB6|E2BL_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-... 74 3e-12
>sp|Q9UZ16|E2B1_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447875|pir||A75044
translation initiation factor aif-2bi PAB2444 -
Pyrococcus abyssi (strain Orsay)
>gi|5458759|emb|CAB50246.1| (AJ248287) translation
initiation factor aIF-2BI [Pyrococcus abyssi]
Length = 356
Score = 716 bits (1827), Expect = 0.0
Identities = 356/356 (100%), Positives = 356/356 (100%)
Query: 1 MEVRYKPEELTKLPRSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAP 60
MEVRYKPEELTKLPRSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAP
Sbjct: 1 MEVRYKPEELTKLPRSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAP 60
Query: 61 AIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEH 120
AIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEH
Sbjct: 61 AIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEH 120
Query: 121 LEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGNLLTHCNAGSLATVHLGTV 180
LEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGNLLTHCNAGSLATVHLGTV
Sbjct: 121 LEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGNLLTHCNAGSLATVHLGTV 180
Query: 181 GAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVD 240
GAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVD
Sbjct: 181 GAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVD 240
Query: 241 AIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERS 300
AIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERS
Sbjct: 241 AIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERS 300
Query: 301 PEEVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKLFGEQ 356
PEEVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKLFGEQ
Sbjct: 301 PEEVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKLFGEQ 356
>sp|O58433|E2B1_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447867|pir||G71116
probable translation initiation factor eIF-2 beta chain
- Pyrococcus horikoshii >gi|3257110|dbj|BAA29793.1|
(AP000003) 364aa long hypothetical translation
initiation factor eIF-2 beta chain [Pyrococcus
horikoshii]
Length = 364
Score = 658 bits (1680), Expect = 0.0
Identities = 323/353 (91%), Positives = 337/353 (94%)
Query: 1 MEVRYKPEELTKLPRSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAP 60
ME+RY P+ELTKLPR+VEYK ++VYMINQRLLP+EFKVE F VE VAEAIKNMTVRGAP
Sbjct: 1 MEIRYTPKELTKLPRTVEYKNKSVYMINQRLLPKEFKVEKFSKVEEVAEAIKNMTVRGAP 60
Query: 61 AIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEH 120
AIGAAA FGLALYAETSKAK+K+EF+DGF KAYE LKNTRPTAVNLFWALNRIKKLVEEH
Sbjct: 61 AIGAAAGFGLALYAETSKAKTKEEFLDGFEKAYEILKNTRPTAVNLFWALNRIKKLVEEH 120
Query: 121 LEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGNLLTHCNAGSLATVHLGTV 180
EDPLDEIK LIV EA KIADEDVEANLRMGHYGAEVLPEGN+LTHCNAGSLATVHLGTV
Sbjct: 121 SEDPLDEIKRLIVQEAYKIADEDVEANLRMGHYGAEVLPEGNILTHCNAGSLATVHLGTV 180
Query: 181 GAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVD 240
G+VVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQG VD
Sbjct: 181 GSVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGFVD 240
Query: 241 AIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERS 300
AIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDM LKSGK+IPIEERS
Sbjct: 241 AIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMELKSGKDIPIEERS 300
Query: 301 PEEVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKLF 353
PEEVLTCGGCRIAPDVPVYNPAFDVTPHKY+TGIITD+ VVWPPFKRNLKKLF
Sbjct: 301 PEEVLTCGGCRIAPDVPVYNPAFDVTPHKYLTGIITDRGVVWPPFKRNLKKLF 353
>sp|Q9X013|E2B_THEMA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447871|pir||F72319 translation
initiation factor IF-2B alpha subunit-related TM0911
[similarity] - Thermotoga maritima (strain MSB8)
>gi|4981447|gb|AAD35992.1|AE001755_15 (AE001755)
translation initiation factor, aIF-2B alpha
subunit-related [Thermotoga maritima]
Length = 343
Score = 328 bits (833), Expect = 5e-89
Identities = 174/340 (51%), Positives = 238/340 (69%), Gaps = 7/340 (2%)
Query: 15 RSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYA 74
+++E+ ++ +++QR LP + +T E VA AIK M VRGAPAIG AAAFG L
Sbjct: 6 KTMEWSGNSLKLLDQRKLPFIEEYVECKTHEEVAHAIKEMIVRGAPAIGVAAAFGYVLGL 65
Query: 75 ETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVN 134
K S ++M + ETL TRPTAVNLFWALNR++K+ E+ + + + ++ N
Sbjct: 66 RDYKTGSLTDWMK---QVKETLARTRPTAVNLFWALNRMEKVFFENADR--ENLFEILEN 120
Query: 135 EAQKIADEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDGSL 193
EA K+A ED+E N +G GA+++ +G+ +LTHCNAG+LATV GT V+R + G
Sbjct: 121 EALKMAYEDIEVNKAIGKNGAQLIKDGSTILTHCNAGALATVDYGTALGVIRAAVESGKR 180
Query: 194 KLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANG 253
++ DETRP LQGARL+AWE DG+ V +I DN A ++M++GL+DA++VGADRI NG
Sbjct: 181 IRVFADETRPYLQGARLTAWELMKDGIEVYVITDNMAGWLMKRGLIDAVVVGADRIALNG 240
Query: 254 DFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIA 313
D ANKIGTY LAVLA+ + IPF+ AP+S+ID +++SG+EIPIEER PEEV CGG RIA
Sbjct: 241 DTANKIGTYSLAVLAKRNNIPFYVAAPVSTIDPTIRSGEEIPIEERRPEEVTHCGGNRIA 300
Query: 314 PD-VPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKL 352
P+ V V NPAFDVT + +T IIT+K V+ PPF+ N+KK+
Sbjct: 301 PEGVKVLNPAFDVTENTLITAIITEKGVIRPPFEENIKKI 340
>sp|O67879|E2B_AQUAE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7519844|pir||C70481 translation
initiation factor eIF-2B alpha chain - Aquifex aeolicus
>gi|2984318|gb|AAC07840.1| (AE000773) initiation factor
eIF-2B alpha subunit [Aquifex aeolicus]
Length = 456
Score = 309 bits (782), Expect = 4e-83
Identities = 164/327 (50%), Positives = 214/327 (65%), Gaps = 19/327 (5%)
Query: 19 YKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSK 78
+K + +++QR LP + +T E VA+AIK M VRGAPAIG AA+G L + K
Sbjct: 138 WKGDKLLLLDQRKLPHQEVWLELKTYEEVAKAIKEMAVRGAPAIGCTAAYGFVLGVKVQK 197
Query: 79 AKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEAQK 138
+ K YETLKNTRPTA NLFWAL+R+ K ++E + I EA++
Sbjct: 198 EDPE--------KVYETLKNTRPTAYNLFWALDRMMKALKEGKD---------IEEEAKE 240
Query: 139 IADEDVEANLRMGHYGAEVLP-EGNLLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLW 197
I ED EAN RMG G+E++P +LTHCN G+LAT GT V+R H G +W
Sbjct: 241 IEKEDYEANKRMGEIGSEIVPLNAKVLTHCNTGALATAGWGTALGVIRSAHYGGKNIFVW 300
Query: 198 LDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFAN 257
+DETRP LQGARL+AWE + + K+I D+ A F+M++G+VD +IVGADRI A GD AN
Sbjct: 301 VDETRPYLQGARLTAWELVKESIPHKIITDSTAGFLMKKGMVDLVIVGADRITAKGDVAN 360
Query: 258 KIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIAP-DV 316
KIGTY LAVL +EH +PF+ AP S+ID ++KSG+EI IEERSPEEV CGGC IAP +
Sbjct: 361 KIGTYTLAVLCKEHNVPFYVAAPTSTIDSNIKSGEEIIIEERSPEEVKVCGGCAIAPKES 420
Query: 317 PVYNPAFDVTPHKYVTGIITDKRVVWP 343
+ AFD+TP + +TGIIT+K + P
Sbjct: 421 DALHLAFDITPAELITGIITEKGIFKP 447
>sp|P74497|E2B_SYNY3 PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447869|pir||S76472 hypothetical
protein - Synechocystis sp. (strain PCC 6803)
>gi|1653689|dbj|BAA18601.1| (D90915) hypothetical
protein [Synechocystis sp.]
Length = 351
Score = 286 bits (724), Expect = 3e-76
Identities = 156/332 (46%), Positives = 211/332 (62%), Gaps = 6/332 (1%)
Query: 17 VEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAET 76
V++++ V +I+Q LP E+K A E++ AIK+M VRGAPAIG AAA+G+ L A
Sbjct: 13 VQWRDDQVILIDQTRLPLEYKEVAIADYEAMGHAIKSMVVRGAPAIGVAAAYGMYLGARR 72
Query: 77 SKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEA 136
+ EF+ + L+ TRPTAVNLFWA++++ V H + +++IK+ ++++A
Sbjct: 73 IQTTELREFVVQLEFVADQLRQTRPTAVNLFWAIDQMLN-VAYHSGENVEQIKANLLSQA 131
Query: 137 QKIADEDVEANLRMGHYGAEVLPEG----NLLTHCNAGSLATVHLGTVGAVVRVMHKDGS 192
Q+I +D+ +G G EVLP +LTHCNAG+LAT GT V+R G
Sbjct: 132 QQIQLDDLRTCQAIGAAGLEVLPREPHQLTILTHCNAGALATAGYGTAIGVIRAAWSAGR 191
Query: 193 LKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVAN 252
L ++ DETRP LQGA+L+ WE G+ V LI DN AA MQQ +DA++VGADRI N
Sbjct: 192 LARVYADETRPRLQGAKLTVWECVQAGIPVTLITDNMAAHCMQQQRIDAVVVGADRIARN 251
Query: 253 GDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRI 312
GD ANKIGTY LAVLA+ H IPFF APLS++D +L G +IPIEER P E+ G RI
Sbjct: 252 GDIANKIGTYGLAVLAKMHAIPFFVAAPLSTVDFALDDGSQIPIEERDPVEIYQPEGNRI 311
Query: 313 APD-VPVYNPAFDVTPHKYVTGIITDKRVVWP 343
P+ YNPAFDVTP +T IIT++ + P
Sbjct: 312 TPEGAEFYNPAFDVTPASLITAIITEQGAIAP 343
>pir||A83249 probable initiation factor 2 subunit PA3169 [imported] -
Pseudomonas aeruginosa (strain PAO1)
>gi|9949286|gb|AAG06557.1|AE004741_2 (AE004741) probable
initiation factor 2 subunit [Pseudomonas aeruginosa]
Length = 358
Score = 285 bits (721), Expect = 6e-76
Identities = 155/347 (44%), Positives = 222/347 (63%), Gaps = 8/347 (2%)
Query: 15 RSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYA 74
+++E+++ T+ +++QRLLP+E + VA+AI++M VRGAPAIG +AA+G+ L A
Sbjct: 12 KAIEWRDGTLRLLDQRLLPQEEVWLEHESAAEVAKAIRDMVVRGAPAIGISAAYGIVLGA 71
Query: 75 ETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVN 134
A+ D + + + L ++RPTAVNLFWALNR++ +E E D+ +++
Sbjct: 72 RARLAQGGD-WRAALEEDFRLLADSRPTAVNLFWALNRMRDRLERMKEG--DQPLAVLEA 128
Query: 135 EAQKIADEDVEANLRMGHYGAEVLPEG-----NLLTHCNAGSLATVHLGTVGAVVRVMHK 189
EA I + D EANL M G E++ + N+LTHCN G+LAT GT V+R H
Sbjct: 129 EAISIHESDREANLTMAQLGMELIRKQQGSPQNILTHCNTGALATGGFGTALGVIRAAHL 188
Query: 190 DGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRI 249
+G + ++ DETRP LQG+RL+AWE + +G+ V L D+AAA +M+ + +IVGADRI
Sbjct: 189 EGLVNRIYADETRPWLQGSRLTAWELANEGIPVSLNVDSAAAHLMKTENITWVIVGADRI 248
Query: 250 VANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGG 309
ANGD ANKIGTY LAV A HG+ F VAP S+IDM+L+SG++IPIEER E+L GG
Sbjct: 249 TANGDVANKIGTYQLAVNAMHHGVRFMVVAPSSTIDMNLESGEDIPIEERDGRELLEIGG 308
Query: 310 CRIAPDVPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKLFGEQ 356
R+A +V YNP FDVTP + I+T++ VV P + L +
Sbjct: 309 RRVAAEVDAYNPVFDVTPADLIDAIVTERGVVERPDAERMAALMSRK 355
>sp|O31662|E2B_BACSU PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447872|pir||C69863 translation
initiation factor IF-2B alpha chain homolog ykrS
[similarity] - Bacillus subtilis
>gi|2633726|emb|CAB13228.1| (Z99111) similar to
initiation factor eIF-2B (alpha subunit) [Bacillus
subtilis]
Length = 353
Score = 281 bits (712), Expect = 7e-75
Identities = 156/343 (45%), Positives = 218/343 (63%), Gaps = 4/343 (1%)
Query: 13 LPRSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLAL 72
+PRSVE+KE + ++NQ+ LP E + T E V +AI + VRGAPAIG AAFGLAL
Sbjct: 7 VPRSVEWKETAITILNQQKLPDETEYLELTTKEDVFDAIVTLKVRGAPAIGITAAFGLAL 66
Query: 73 YAETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLI 132
A+ + + EF + L ++RPTA+NL WAL R+ VE + ++E K+ +
Sbjct: 67 AAKDIETDNVTEFRRRLEDIKQYLNSSRPTAINLSWALERLSHSVENAIS--VNEAKTNL 124
Query: 133 VNEAQKIADEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDG 191
V+EA +I ED E +G ++ +G+ ++T CNAGS+AT GT A + +
Sbjct: 125 VHEAIQIQVEDEETCRLIGQNALQLFKKGDRIMTICNAGSIATSRYGTALAPFYLAKQKD 184
Query: 192 SLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVA 251
++ ETRPVLQG+RL+AWE G++V LI D+ AA M++ + A+IVGADRI
Sbjct: 185 LGLHIYACETRPVLQGSRLTAWELMQGGIDVTLITDSMAAHTMKEKQISAVIVGADRIAK 244
Query: 252 NGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCR 311
NGD ANKIGTY LA+LA IPFF APLS+ D +K G +IPIEER PEEV G R
Sbjct: 245 NGDTANKIGTYGLAILANAFDIPFFVAAPLSTFDTKVKCGADIPIEERDPEEVRQISGVR 304
Query: 312 IAP-DVPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKLF 353
AP +VPV+NPAFD+TPH ++GIIT+K ++ ++ +++LF
Sbjct: 305 TAPSNVPVFNPAFDITPHDLISGIITEKGIMTGNYEEEIEQLF 347
>gi|11497982 translation initiation factor eIF-2B, subunit delta (eif2BD)
[Archaeoglobus fulgidus]
>gi|3182987|sp|O29877|E2B1_ARCFU PUTATIVE TRANSLATION
INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447868|pir||B69296 translation
initiation factor eIF-2B, subunit delta (eif2BD) homolog
- Archaeoglobus fulgidus >gi|2650263|gb|AAB90865.1|
(AE001079) translation initiation factor eIF-2B, subunit
delta (eif2BD) [Archaeoglobus fulgidus]
Length = 339
Score = 278 bits (704), Expect = 6e-74
Identities = 153/329 (46%), Positives = 219/329 (66%), Gaps = 6/329 (1%)
Query: 26 MINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEF 85
+I+Q LP + +V R VE +A+AIK + VRGAPA+ AA A+G+AL A + DE
Sbjct: 12 LIDQTKLPEKLEVIECRNVEELADAIKKLAVRGAPALEAAGAYGIALAAREREFADVDEL 71
Query: 86 MDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEAQKIADEDVE 145
+ KA + L +TRPTAVNLF + R + + ++E+K L + EA+K+A+EDVE
Sbjct: 72 KEHLKKAADFLASTRPTAVNLFVGIERALNAALKG--ESVEEVKELALREAEKLAEEDVE 129
Query: 146 ANLRMGHYGAEVLPEGNL-LTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPV 204
N +MG YGAE+L +G++ LT+CNAG LATV GT VVR + G + ETRP+
Sbjct: 130 RNRKMGEYGAELLEDGDVVLTYCNAGRLATVDWGTALGVVRSAVEQGKEIRVIACETRPL 189
Query: 205 LQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYML 264
QG+RL+ WE DG++V LI D+ VMQ+G+VD +IVGADRIV + F NKIGTY +
Sbjct: 190 NQGSRLTCWELMEDGIDVTLITDSMVGIVMQKGMVDKVIVGADRIVRDAVF-NKIGTYTV 248
Query: 265 AVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIAP-DVPVYNPAF 323
+V+A+ H IPF+ AP ++ D ++ K++ IEER EE++ CG +IAP +V VYNPAF
Sbjct: 249 SVVAKHHNIPFYVAAPKATFDWE-RTAKDVVIEERPREELIFCGKRQIAPLNVKVYNPAF 307
Query: 324 DVTPHKYVTGIITDKRVVWPPFKRNLKKL 352
D TP + VT +IT+ V++PP++ N+ K+
Sbjct: 308 DPTPLENVTALITEYGVIYPPYEVNVPKV 336
>sp|Q57896|E2B1_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|2129325|pir||F64356
translation initiation factor aIF-2B alpha chain -
Methanococcus jannaschii >gi|1591158|gb|AAB98443.1|
(U67496) translation initiation factor aIF-2B, subunit
delta (aIF2BD) [Methanococcus jannaschii]
Length = 329
Score = 276 bits (699), Expect = 2e-73
Identities = 162/335 (48%), Positives = 220/335 (65%), Gaps = 22/335 (6%)
Query: 21 ERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAK 80
++ + +I+QR LP + + +T E VA AIK+M VRGAPAIG +AA+GLAL AE +
Sbjct: 14 KKELILIDQRKLPNKLEYFICKTYEDVAYAIKDMVVRGAPAIGVSAAYGLAL-AEING-- 70
Query: 81 SKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEAQKIA 140
D YKAYE LKNTRPTAVNLFWAL+R +E I++EA+KI
Sbjct: 71 ------DDIYKAYEVLKNTRPTAVNLFWALDRCLTAYKEGKS---------ILDEAKKIH 115
Query: 141 DEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLD 199
+ED+E ++G G +++ +G+ +LTHCNAG+LAT GT +V+R +G + D
Sbjct: 116 EEDIETCKKIGMIGEKLIEDGDTILTHCNAGALATSAYGTALSVIRFAFYNGKKIRVIAD 175
Query: 200 ETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKI 259
ETRP LQGA+L+A+E +Y+G+ VK+I DN A F+MQ+G +D IIVGADRI+A+G NKI
Sbjct: 176 ETRPRLQGAKLTAFELNYEGIPVKVITDNTAGFLMQKGEIDKIIVGADRILADGTVYNKI 235
Query: 260 GTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIAPD-VPV 318
GTY LAVLA+ H IPF+ APLS+ D+ S +++ IEER +EV G RI P+ V
Sbjct: 236 GTYSLAVLAKYHRIPFYVAAPLSTFDLR-SSEEDVIIEERDEKEVAYIDGVRIVPEGVGC 294
Query: 319 YNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKLF 353
YN AFD TP +T IIT+K +V P + + KLF
Sbjct: 295 YNYAFDKTPPDLITAIITEKGIV-KPNRDEILKLF 328
>emb|CAC12337.1| (AL445066) translation initiation factor aIF-2B, alpha chain
related protein [Thermoplasma acidophilum]
Length = 340
Score = 272 bits (688), Expect = 5e-72
Identities = 156/340 (45%), Positives = 216/340 (62%), Gaps = 24/340 (7%)
Query: 15 RSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYA 74
++V Y++ V +I+QR LP + ++ + + +A AIKNM VRGAPAIG AA+GLA+
Sbjct: 13 KAVWYEDDEVKLIDQRKLPEKIEIFEAKNSDDIAYAIKNMVVRGAPAIGVTAAYGLAM-- 70
Query: 75 ETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVN 134
AK K E MD +A + +TRPTA +LF A+ + +I +N
Sbjct: 71 ----AKKKGENMD---EAVRKIGSTRPTAYDLFKAIRYM-------------QINDFDMN 110
Query: 135 EAQKIADEDVEANLRMGHYGAEVLPEG-NLLTHCNAGSLATVHLGTVGAVVRVMHKDGSL 193
A++ A E + ++G G +++ + +LTHCNAG+LA V GT A +R+ H DG
Sbjct: 111 SARRYAMEISGRSRKIGEIGNQLIGQNARILTHCNAGALAVVDWGTALAPIRMAHGDGKN 170
Query: 194 KLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANG 253
+++DETRP LQGA+L+AWE + +G++ +IADNAA F M++ VD +IVGADRI +NG
Sbjct: 171 IFVFVDETRPRLQGAKLTAWELAQEGIDHAIIADNAAGFYMRRKEVDLVIVGADRIASNG 230
Query: 254 DFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIA 313
DFANKIGTY AVLA+ +GIPF+ AP S+ D S+KSG EIPIEER EVL G RI
Sbjct: 231 DFANKIGTYEKAVLAKTNGIPFYVAAPGSTFDFSIKSGDEIPIEERDENEVLEINGTRIG 290
Query: 314 P-DVPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKL 352
P + NPAFDVTP++YVTG IT+ + P LK+L
Sbjct: 291 PVESHSRNPAFDVTPNEYVTGFITEYGIFKPSELGKLKEL 330
>gb|AAK43082.1| Translation initiation factor aiF-2B, subunit 1 (eiF2B) [Sulfolobus
solfataricus]
Length = 366
Score = 266 bits (674), Expect = 2e-70
Identities = 158/356 (44%), Positives = 225/356 (62%), Gaps = 12/356 (3%)
Query: 5 YKPEELTKLPRSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGA 64
YKP+ LP + + T+ +++Q LLP + + V+S A AIKNM VRGAPAIG
Sbjct: 12 YKPK---LLPIIWKDENNTLTILDQSLLPFQKVYVDLKDVDSTALAIKNMQVRGAPAIGI 68
Query: 65 AAAFGLALYAETSK-AKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLED 123
A +G+ L ++K K+ DE + +A L + RPTAVNL WA +R+ L + +E+
Sbjct: 69 TAGYGMVLALTSNKNVKTLDEAIRELTRAKAILDSARPTAVNLVWATSRMLTLAKNAVEN 128
Query: 124 ----PLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLAT-VHL 177
++E+ L+ EA++I DE+ +A ++MG YG E L + + +LT CNAG LAT L
Sbjct: 129 GNAKTINELIDLMKVEAKRIFDEEYDAEIQMGLYGLEKLNDSDTVLTQCNAGGLATGTGL 188
Query: 178 GTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQG 237
GT + V++ G + ETRP LQG+RL+ +E +G+ V LI D A VM +G
Sbjct: 189 GTALSPVKLAKALGMNVSVIAPETRPWLQGSRLTVYELMEEGIKVTLITDTAVGLVMYRG 248
Query: 238 LVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIE 297
+V++++VGADRI+ +G NKIGTY AV+A E GIPF+A+AP SS D+ + EI IE
Sbjct: 249 MVNSVMVGADRILRDGHVFNKIGTYKEAVIAHELGIPFYALAPTSSFDLK-SNVNEIKIE 307
Query: 298 ERSPEEVLTCGGCRIAPD-VPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKKL 352
ER P EV T G IAP+ V VYNP FDVTP KY+ GIIT+K +++PPF N+K++
Sbjct: 308 ERDPNEVRTIRGIPIAPENVNVYNPVFDVTPPKYIAGIITEKGIIYPPFHENIKRI 363
>sp|O27900|E2B1_METTH PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447870|pir||E69117
translation initiation factor eIF-2B, alpha subunit -
Methanobacterium thermoautotrophicum (strain Delta H)
>gi|2623009|gb|AAB86338.1| (AE000939) translation
initiation factor eIF-2B, alpha subunit
[Methanobacterium thermoautotrophicum]
Length = 311
Score = 261 bits (659), Expect = 1e-68
Identities = 151/331 (45%), Positives = 207/331 (61%), Gaps = 26/331 (7%)
Query: 15 RSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYA 74
+++E+K+ + +I+QR LP + R V AIKNM VRGAPAIG AAFG+A+
Sbjct: 4 KTIEWKDGELVLIDQRKLPDSLEYFRCRDYRDVIYAIKNMVVRGAPAIGVTAAFGVAM-- 61
Query: 75 ETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVN 134
A+ E ++ A E +K +RPTAVNLFWA++R+ K E PLDE ++ +
Sbjct: 62 ----AELAGEDVE---VAAEEIKASRPTAVNLFWAVDRVMKS-----ESPLDEAITMYM- 108
Query: 135 EAQKIADEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDGSL 193
ED+E N +G +GA ++ +G+ +LTHCNAG+LA V GT V+R DG
Sbjct: 109 -------EDMETNRAIGRHGAGLIEDGDTVLTHCNAGALACVDYGTALGVIRAAWDDGKR 161
Query: 194 KLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANG 253
+ DETRPV QGARLS WE +G+ VKLI D AA ++MQ G++D +I+GADRI A G
Sbjct: 162 LTVICDETRPVGQGARLSVWEMQQEGIPVKLITDVAAGYLMQTGMIDKVIIGADRI-AEG 220
Query: 254 DFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIA 313
ANKIG+ M+A+ A+ +PF+ AP+S+ D S +I IEER P EVL GGCRIA
Sbjct: 221 GVANKIGSLMVALAAKRFNVPFYVAAPMSTFDTE-NSIYDIEIEERDPAEVLYYGGCRIA 279
Query: 314 P-DVPVYNPAFDVTPHKYVTGIITDKRVVWP 343
P D NPAFD+ P ++GIIT+K ++ P
Sbjct: 280 PQDTEAINPAFDIVPSDLISGIITEKGILDP 310
>sp|Q9PAG5|E2B_XYLFA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|11279300|pir||A82544 translation
initiation factor IF-2B alpha subunit-related XF2553
[imported] - Xylella fastidiosa (strain 9a5c)
>gi|9107764|gb|AAF85350.1|AE004062_5 (AE004062)
initiation factor eIF-2B, alpha subunit-related [Xylella
fastidiosa]
Length = 354
Score = 259 bits (654), Expect = 4e-68
Identities = 144/331 (43%), Positives = 198/331 (59%), Gaps = 6/331 (1%)
Query: 26 MINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEF 85
+++QR LP + + E V +AI+ + VRGAPAIG A +G L A +A E
Sbjct: 27 LLDQRKLPFVVEYVECHSSEDVTQAIRALIVRGAPAIGIVAGWGAVLAAREIEAVDGIEA 86
Query: 86 MDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEAQKIADEDVE 145
+ A + L RPTAVNL W L R+++ + D + + EA+ IA ED+
Sbjct: 87 LRKLEPALQRLHAARPTAVNLAWVLARMRRTLSAAHAD----WRQAMAREAESIAREDLT 142
Query: 146 ANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPV 204
AN MG YGA ++P G+ +LTHCN GSLAT GT V+R G + ++ ETRP
Sbjct: 143 ANRCMGAYGAALIPIGSGVLTHCNTGSLATAGFGTALGVIRAGIAQGRIARVFAGETRPW 202
Query: 205 LQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYML 264
LQGARL+ WE DG++V LIAD+AAA +M+ G V +IVGADRI ANGD ANKIGTY L
Sbjct: 203 LQGARLTVWELQQDGIDVTLIADSAAAHLMKSGQVQWVIVGADRICANGDAANKIGTYQL 262
Query: 265 AVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCR-IAPDVPVYNPAF 323
A+ AR HG+ F VA +++DM +G+ I IE+R PEE+L G R +A + +NP F
Sbjct: 263 AITARHHGVKFMVVASAATVDMDTTAGEAIEIEQRDPEELLGVSGVRTVAEGIAAWNPVF 322
Query: 324 DVTPHKYVTGIITDKRVVWPPFKRNLKKLFG 354
DVTP + I+T++ V+ P ++ FG
Sbjct: 323 DVTPGALIDAIVTERGVIQSPDAAQMRATFG 353
>sp|Q9YE84|E2B1_AERPE PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 1 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447874|pir||F72657
probable translation initiation factor eIF-2B alpha
subunit APE0686 [imported] - Aeropyrum pernix (strain
K1) >gi|5104347|dbj|BAA79662.1| (AP000060) 370aa long
hypothetical translation initiation factor eFI-2B alpha
subunit [Aeropyrum pernix]
Length = 370
Score = 251 bits (634), Expect = 1e-65
Identities = 146/346 (42%), Positives = 201/346 (57%), Gaps = 10/346 (2%)
Query: 14 PRSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALY 73
P ++ E ++ RL+P VA AI +M +RGAPAIG AAA+ LAL
Sbjct: 23 PLYFDFDEGVFVWLDTRLIPFREVYRRTSDYRRVARAIVDMEIRGAPAIGVAAAYALALA 82
Query: 74 AETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLE-DPLDEIKSLI 132
A + ++ + F++ +A +++TRPTA NLFWA R+ V E +D
Sbjct: 83 AAEAASRGGEGFIEALSEARREIESTRPTAYNLFWATARVYGAVSEAFRRSGVDAAVRAG 142
Query: 133 VNEAQKIADEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDG 191
+ EA +I EDV N+ +G GA +L G+ +LTHCN G+LAT GT V+R +G
Sbjct: 143 LEEATRIYVEDVRGNVEIGRVGARLLESGDTVLTHCNTGALATAGFGTALGVIRYAWMEG 202
Query: 192 SLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVA 251
+ ETRPVLQGARL+ WE +G+ KL+ D+A +M +G+V IVGADRIV+
Sbjct: 203 KDIRVITTETRPVLQGARLNVWELRKEGIPFKLVVDSAVGLIMSRGMVSKAIVGADRIVS 262
Query: 252 NGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKE-IPIEERSPEEV----LT 306
G ANKIGTYM+A+ A HG+PF+ AP S+ +G E I IEERSP+EV
Sbjct: 263 TGHTANKIGTYMVAMAASRHGVPFYVAAPASTFQPD--AGPEAIVIEERSPDEVRGVISE 320
Query: 307 CGGCRIA-PDVPVYNPAFDVTPHKYVTGIITDKRVVWPPFKRNLKK 351
G RI DV YNP+FDVTP + +T ITD+ V+ PPF N+++
Sbjct: 321 AGYVRITLGDVEAYNPSFDVTPPELITAFITDRGVIEPPFDVNIRR 366
>gb|AAC95160.1| (AC005970) putative translation initiation factor eIF-2B alpha
subunit [Arabidopsis thaliana]
Length = 365
Score = 244 bits (617), Expect = 1e-63
Identities = 146/343 (42%), Positives = 213/343 (61%), Gaps = 23/343 (6%)
Query: 15 RSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLAL-- 72
+++ YK ++ +++QR LP E R AI+ M VRGAPAI AAA LA+
Sbjct: 10 KAICYKPGSLQLLDQRKLPLETIYLEIRDASDGWSAIQEMVVRGAPAIAIAAALSLAVEV 69
Query: 73 ---YAETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIK 129
+ A F++ + L ++RPTAVNL A ++K ++ + L E K
Sbjct: 70 FNFHGFDGSASDAVAFLEN---KLDYLVSSRPTAVNLADAALKLKHVIAKALATAT-EAK 125
Query: 130 SLI---VNEAQKIADEDVEANLRMGHYGAEVLPEG-------NLLTHCNAGSLATVHLGT 179
S+ + ++ + ++DV +N +G++G +L + ++LTHCN GSLAT GT
Sbjct: 126 SIFKAYIEASEDMLEDDVVSNKAIGNFGLSLLRQQAKNPDKLSVLTHCNTGSLATAGYGT 185
Query: 180 VGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLV 239
V+R +H G L+ + ETRP QG+RL+A+E ++ + LIAD+AAA +M+ G V
Sbjct: 186 ALGVIRALHTQGILERAYCTETRPFNQGSRLTAFELVHEKIPATLIADSAAAALMKDGRV 245
Query: 240 DAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEER 299
D +IVGADR+ +NGD ANKIGTY LA+ A+ HGIPF+ APL+S+D+SL SGKEI IEER
Sbjct: 246 DGVIVGADRVASNGDTANKIGTYSLALCAKHHGIPFYVAAPLTSVDLSLSSGKEIVIEER 305
Query: 300 SPEEVL-TCGGC--RI-APDVPVYNPAFDVTPHKYVTGIITDK 338
SP+E++ T GG RI AP + V+NPAFD+TP + + GIIT+K
Sbjct: 306 SPKELMHTHGGLGERIAAPGISVWNPAFDMTPAELIAGIITEK 348
>pir||T39944 hypothetical translation initiation factor - fission yeast
(Schizosaccharomyces pombe) >gi|3116128|emb|CAA18878.1|
(AL023287) translation initiation factor eif-2 family
[Schizosaccharomyces pombe]
Length = 359
Score = 220 bits (554), Expect = 2e-56
Identities = 136/352 (38%), Positives = 199/352 (55%), Gaps = 38/352 (10%)
Query: 15 RSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYA 74
+++ Y E + +++Q LLP E K + VE IK M VRGAPAI AA +A+
Sbjct: 4 QAIIYNEDKLLVLDQLLLPYERKYISVSNVEDAFAVIKKMQVRGAPAIAIVAALSVAVGL 63
Query: 75 E--TSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLI 132
+ + A +K+ ++ F E LK +RPTAVNLF ++ + + ++ ++ I
Sbjct: 64 KHLSEDADAKEYVINSF----EHLKQSRPTAVNLFETAKFMQGIALQEGKN----CRNKI 115
Query: 133 VNEAQKIADEDVEANLRMGHYGAEVLPEG-------NLLTHCNAGSLATVHLGTVGAVVR 185
+ EA+++ +D+E N +G G + L + +LTHCN GSLAT GT ++R
Sbjct: 116 IQEAERMLVKDLEDNHNIGTAGRKFLLQHYKDKDKLTVLTHCNTGSLATSGYGTALGIIR 175
Query: 186 VMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVG 245
+H+ G+L+ + ETRP QG+RL+A+E +D + L+ D+ A +M + +DAI+VG
Sbjct: 176 SLHESGNLEHAYCTETRPYNQGSRLTAFELVHDKIPATLVTDSTVASIMHK--IDAIVVG 233
Query: 246 ADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVL 305
ADR+ NGD ANKIGTY LA+LA+ G PF AP SSID SL SG +I IE+R P E+
Sbjct: 234 ADRVTRNGDTANKIGTYNLAILAKHFGKPFIVAAPFSSIDTSLASGSQITIEQRPPIEMT 293
Query: 306 TCGGCRI-------------------APDVPVYNPAFDVTPHKYVTGIITDK 338
T G I AP + VY+PAFDVTP +T I T+K
Sbjct: 294 TITGPIITDSNSRNLEDPKRVRISIAAPGIDVYSPAFDVTPANLITAIATEK 345
>gb|AAF57154.1| (AE003778) CG11334 gene product [alt 1] [Drosophila melanogaster]
>gi|7302053|gb|AAF57155.1| (AE003778) CG11334 gene
product [alt 2] [Drosophila melanogaster]
Length = 364
Score = 216 bits (544), Expect = 4e-55
Identities = 127/348 (36%), Positives = 192/348 (54%), Gaps = 19/348 (5%)
Query: 15 RSVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYA 74
+S++Y ++ +++Q LLP + K R VE + I M VRGAPAI LA+
Sbjct: 4 QSIKYSRGSLEILDQLLLPGQSKYVVVRGVEDGWKVINKMQVRGAPAIAIVGCLSLAVEI 63
Query: 75 ETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDP---LDEIKSL 131
++K L + RPTAVN+ A + + L E +D + ++K
Sbjct: 64 NPEDFENKKSLRQEIEGKLNYLVSARPTAVNMKIAADELITLANELYKDEAIDVTQMKHR 123
Query: 132 IVNEAQKIADEDVEANLRMGHYGAEVLPEG---------------NLLTHCNAGSLATVH 176
++ + + +D+ N +G GA+ + +G +LTHCN GSLAT
Sbjct: 124 FLDATEAMLKKDIADNRAIGANGAQAILQGVAKAGKAPAGSTGSVRVLTHCNTGSLATAG 183
Query: 177 LGTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQ 236
GT VVR + + G L+ ++ ETRP QGARL+A+E ++ L+ D+ A +++
Sbjct: 184 YGTALGVVRQLAELGKLEHVYCTETRPYNQGARLTAYELVHEKFPATLVLDSMVAALLRA 243
Query: 237 GLVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPI 296
V A++VGADR+ +NGD ANKIGTY +AV+A+ H +PF+ APL+SID+++ G I I
Sbjct: 244 KNVAAVVVGADRVASNGDTANKIGTYQIAVVAKHHDVPFYVAAPLTSIDLAIPGGDHIII 303
Query: 297 EERSPEEVLTCGGCRI-APDVPVYNPAFDVTPHKYVTGIITDKRVVWP 343
EER E+ G RI AP + +NPAFDVTP +TGIIT++ V P
Sbjct: 304 EERPDREMTHVGEHRIAAPGINCWNPAFDVTPASLITGIITERGVFKP 351
>emb|CAB90925.1| (AL355774) putative translation initiation factor [Streptomyces
coelicolor A3(2)]
Length = 380
Score = 213 bits (537), Expect = 2e-54
Identities = 135/340 (39%), Positives = 188/340 (54%), Gaps = 33/340 (9%)
Query: 26 MINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEF 85
+++Q LP E ++ EAI+++ VRGAP +G A +G+AL A
Sbjct: 33 LLDQTRLPAEEVELVCTDPAALVEAIRSLAVRGAPLLGIAGGYGVALAA----------- 81
Query: 86 MDGFY--KAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLD--EIKSLIVNEAQKIAD 141
+ GF +A L RPTAVNL + R + E L D + + A+ +
Sbjct: 82 VRGFEVEEAAAALAGARPTAVNLAVGVRRAQAAHREALAGTGDTRQAARAALAAARALHR 141
Query: 142 EDVEANLRMGHYGA----EVLPEGN--LLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKL 195
ED EA+ RM +G E+LP G +LTHCN GSL + GT AV H+ G L+
Sbjct: 142 EDTEASARMAAHGLALLDELLPAGGHRVLTHCNTGSLVSGGEGTAFAVALAAHRSGRLRR 201
Query: 196 LWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDF 255
LW+DETRP+LQGARL+A+E + + + L+ DNAA + G VDA+++GADRI A+G
Sbjct: 202 LWVDETRPLLQGARLTAYEAARNDMAYTLLTDNAAGSLFAAGEVDAVLIGADRIAADGSV 261
Query: 256 ANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTC-------- 307
ANK+G+Y LAVLAR H +PF VAP++++D G I +E+R EV
Sbjct: 262 ANKVGSYPLAVLARYHHVPFVVVAPVTTVDPDTPDGASIEVEQRPGYEVTEVTAPQVPVA 321
Query: 308 ---GGCRIAP-DVPVYNPAFDVTPHKYVTGIITDKRVVWP 343
GG +AP YNPAFDVTP + VT I+T++ VV P
Sbjct: 322 GAGGGIPVAPLGTQAYNPAFDVTPPELVTAIVTEEGVVSP 361
>sp|Q93169|YQJQ_CAEEL HYPOTHETICAL 40.9 KD PROTEIN C01G10.9 IN CHROMOSOME V
>gi|7495123|pir||T18820 hypothetical protein C01G10.9 -
Caenorhabditis elegans >gi|3873873|emb|CAB02711.1|
(Z81030) Strong similarity with yeast hypothetical
protein (Swiss Prot accession number P23140), contains
similarity to Pfam domain: PF01008 (Initiation factor 2
subunit family), Score=490.1, E-value=5.7e-144, N=1~cDNA
EST yk77c8.3 comes from this gene>
Length = 366
Score = 207 bits (521), Expect = 2e-52
Identities = 130/339 (38%), Positives = 194/339 (56%), Gaps = 18/339 (5%)
Query: 16 SVEYKERTVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAE 75
S+++ + +++Q LLP EFK V IK+M VRGAP I + GL L E
Sbjct: 27 SLKFDGTNLEVLDQLLLPHEFKYIPVEGVSDAFAVIKSMQVRGAPLIAVVGSLGLLL--E 84
Query: 76 TSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVE-EHLED--PLDEIKSLI 132
KA D + + L ++RPTAV+L +LN +K ++E + D L++ +S +
Sbjct: 85 IQKASELDS--ESIIQKINFLISSRPTAVDLRNSLNGLKPILESQDYSDVVKLEKCRSYL 142
Query: 133 VNEAQKIADEDVEANLRMGHYGAEVLP------EGNLLTHCNAGSLATVHLGTVGAVVRV 186
+N DE ++ + + + E+L + ++T CN GSLAT+ GT V+R
Sbjct: 143 LNV---YTDEKLQNRILVWNAYQELLSAFPDKEKLTVMTICNTGSLATISWGTALGVIRA 199
Query: 187 MHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAIIVGA 246
+H + LKL+++ ETRP QG RL++ E + + KLI D+ AA+ M+ VD ++ GA
Sbjct: 200 LHSENRLKLVYVLETRPYNQGIRLTSIELLHGEVPFKLITDSMAAWAMKNHQVDCVLTGA 259
Query: 247 DRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPEEVLT 306
D + NGD ANKIGTYMLAVL + H I F+ V P ++I+ ++ +G+EI IEER E+L
Sbjct: 260 DNVARNGDTANKIGTYMLAVLCKHHNINFYPVVPFTTINKNIATGEEIKIEERPSAEMLR 319
Query: 307 CGGCRIA-PDVPVYNPAFDVTPHKYVTGIITDKRVVWPP 344
G I + PV+NPAFDVTP +T I+TD WPP
Sbjct: 320 VNGVLIGNSECPVWNPAFDVTPAHLITKILTDFG-NWPP 357
>gi|6325375 Ypr118wp [Saccharomyces cerevisiae]
>gi|3183489|sp|Q06489|YP18_YEAST HYPOTHETICAL 45.0 KD
PROTEIN IN PIS1-CLB2 INTERGENIC REGION
>gi|2133029|pir||S69011 translation initiation factor
eIF-2B alpha chain homolog YPR118w - yeast
(Saccharomyces cerevisiae) >gi|1066474|gb|AAB68059.1|
(U40828) P9642.7 gene product [Saccharomyces cerevisiae]
Length = 411
Score = 180 bits (452), Expect = 2e-44
Identities = 129/382 (33%), Positives = 187/382 (48%), Gaps = 66/382 (17%)
Query: 23 TVYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSK 82
+V +++Q LLP K T++ IK+M VRGAPAI + + + K
Sbjct: 17 SVKVLDQLLLPYTTKYVPIHTIDDGYSVIKSMQVRGAPAIAIVGSLSVLTEVQLIKHNPT 76
Query: 83 DEFMDGFY------------KAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKS 130
+ + K + L ++RPTAVNL +L IK +++ + L
Sbjct: 77 SDVATLYSLVNWESTKTVLNKRLDFLLSSRPTAVNLSNSLVEIKNILKSSSD--LKAFDG 134
Query: 131 LIVNEAQKIADEDVEANLRMGHYGAEVL----------PEGNLLTHCNAGSLATVHLGTV 180
+ N ++ DED+ N++MG GA+ L E +LT CN GSLAT GT
Sbjct: 135 SLYNYVCELIDEDLANNMKMGDNGAKYLIDVLQKDGFKDEFAVLTICNTGSLATSGYGTA 194
Query: 181 GAVVRVMHKDGSLKLLWLD-----------------ETRPVLQGARLSAWEYSYDGLNVK 223
V+R + KD K D ETRP QG+RL+A+E YD +
Sbjct: 195 LGVIRSLWKDSLAKTDKADSGLDNEKCPRMGHVFPLETRPYNQGSRLTAYELVYDKIPST 254
Query: 224 LIADNAAAFVMQQGLVD--AIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPL 281
LI D++ A+ ++ + A VGADRIV NGD ANKIGT LAV+ ++ GI FF VAP
Sbjct: 255 LITDSSIAYRIRTSPIPIKAAFVGADRIVRNGDTANKIGTLQLAVICKQFGIKFFVVAPK 314
Query: 282 SSIDMSLKSGKEIPIEERSPEEVLTCGGCRIAP-----------------------DVPV 318
++ID ++G +I +EER+PEE G I P ++ V
Sbjct: 315 TTIDNVTETGDDIIVEERNPEEFKVVTGTVINPENGSLILNESGEPITGKVGIAPLEINV 374
Query: 319 YNPAFDVTPHKYVTGIITDKRV 340
+NPAFD+TPH+ + GIIT++ V
Sbjct: 375 WNPAFDITPHELIDGIITEEGV 396
>sp|Q57586|E2B2_METJA PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|2129320|pir||B64315
translation initiation factor eIF-2B delta chain homolog
- Methanococcus jannaschii >gi|1590893|gb|AAB98103.1|
(U67469) translation initiation factor aIF-2B, subunit
delta, putative [Methanococcus jannaschii]
Length = 308
Score = 154 bits (386), Expect = 1e-36
Identities = 105/309 (33%), Positives = 163/309 (51%), Gaps = 20/309 (6%)
Query: 36 FKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYET 95
FK+ ++ E IKNM +RGA IG AAA L YA +++EF + +A
Sbjct: 3 FKMSEMDIIKETYEKIKNMEIRGAGRIGRAAAKALKEYALKISHLNEEEFKNKMREAGNI 62
Query: 96 LKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGA 155
L + RPTAV+L + + K + E E+P K ++ A + + ++A +G +GA
Sbjct: 63 LISARPTAVSLPNVVKYVLKGLNE--ENP----KERVIERADEFINSSLKAIENIGKFGA 116
Query: 156 EVLPEGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWE 214
+ +G+ +LTHCN+ + +V ++ + +G ++ ETRP QG +
Sbjct: 117 NRIKDGDTILTHCNSEAAISV--------IKTAYDEGKDIKVFCTETRPRNQGYLTAKTL 168
Query: 215 YSYDGLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIP 274
Y Y G++V LI D+A + +++ +D ++VGAD I ANG NKIGT +A++A E +P
Sbjct: 169 YDY-GIDVTLIVDSAVRYFIKE--IDIVVVGADAITANGCLVNKIGTSQIALIANESRVP 225
Query: 275 FFAVAPLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGI 334
F A G+ I IEERSPEEV + + NPAFDVTP KY+ I
Sbjct: 226 FLTAAETYKFHPKTIVGELIEIEERSPEEVAVFED--KYKGIKIRNPAFDVTPAKYIDAI 283
Query: 335 ITDKRVVWP 343
IT+ ++ P
Sbjct: 284 ITEVGLIPP 292
>sp|O57947|E2B2_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447865|pir||F71243
probable translation initiation factor eIF-2B delta -
Pyrococcus horikoshii >gi|3256594|dbj|BAA29277.1|
(AP000001) 324aa long hypothetical translation
initiation factor eIF-2B delta [Pyrococcus horikoshii]
Length = 324
Score = 151 bits (377), Expect = 1e-35
Identities = 108/319 (33%), Positives = 175/319 (54%), Gaps = 18/319 (5%)
Query: 42 RTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRP 101
+ V AE IK+M +RGA I AAA L + AE SKAK +E + A + L NTRP
Sbjct: 7 KEVYETAEKIKSMEIRGAGRIARAAAQALMIQAEKSKAKEPEELWNELKVASKILYNTRP 66
Query: 102 TAVNLFWALNRIKKLVEEHL--EDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLP 159
TAV+L AL + V+ L+ ++ +N A++ +A R+G GA+ +
Sbjct: 67 TAVSLPNALRYVMHRVKAAYLGGADLETLRFTAINSAKEFIYNSEKAIERIGEIGAKRIE 126
Query: 160 EGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYD 218
+G+ ++THC++ + + + + + ++K++ + ETRP QG +++A E +
Sbjct: 127 DGDIIMTHCHSKA-------AISVMKKAFEQGKNIKVI-VTETRPKWQG-KITAKELASY 177
Query: 219 GLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAV 278
G+ V I D+AA M+ + D +++GAD I ANG NKIGT ++A+ A+EH +
Sbjct: 178 GIPVIYIVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIA 235
Query: 279 APLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIAP-DVPVYNPAFDVTPHKYVTGIITD 337
A + G+ + IE R P EV+ R P ++ V+NPAFDVTP +Y+ IIT+
Sbjct: 236 AETYKFHPATMLGQLVEIEMRDPTEVIPEEELRTWPKNIEVWNPAFDVTPPEYIDVIITE 295
Query: 338 KRVVWPPFKR--NLKKLFG 354
+ ++ PP+ LK+ FG
Sbjct: 296 RGII-PPYAAIDILKEEFG 313
>sp|Q9V281|E2B2_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B
GDP-GTP EXCHANGE FACTOR) >gi|7447876|pir||F75208
translation initiation factor aif-2bII translation
initiation factor PAB2402 - Pyrococcus abyssi (strain
Orsay) >gi|5457626|emb|CAB49117.1| (AJ248283)
translation initiation factor aIF-2BII translation
initiation factor [Pyrococcus abyssi]
Length = 324
Score = 147 bits (368), Expect = 2e-34
Identities = 106/319 (33%), Positives = 169/319 (52%), Gaps = 18/319 (5%)
Query: 42 RTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRP 101
R V +AE IK+M +RGA I + A L + AE S AKS +E +A + L TRP
Sbjct: 7 REVYEIAEKIKSMEIRGAGKIARSVAQALMIQAEKSTAKSPEELWTELKEAAKILYKTRP 66
Query: 102 TAVNLFWALNRI--KKLVEEHLEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLP 159
TAV+L AL + + + L+ ++ ++N A++ +A R+G GA+ +
Sbjct: 67 TAVSLPNALRYVMHRAKIAYSSGADLETLRFTVINSAKEFIHNSEKAIERIGEIGAKRIE 126
Query: 160 EGN-LLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYD 218
+G+ ++THC H +V++ G + + ETRP QG +++A E +
Sbjct: 127 DGDVIMTHC--------HSKAAISVMKTAFDQGKDIKVIVTETRPRWQG-KITAKELASY 177
Query: 219 GLNVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAV 278
G+ V + D+AA M+ + D +++GAD I ANG NKIGT ++A+ A+EH +
Sbjct: 178 GIPVIYVVDSAARHYMK--MTDKVVMGADSITANGAVINKIGTSLIALTAKEHRVWVMIA 235
Query: 279 APLSSIDMSLKSGKEIPIEERSPEEVLTCGGCRIAP-DVPVYNPAFDVTPHKYVTGIITD 337
A G+ + IE R P EV+ + P ++ V+NPAFDVTP +Y+ IIT+
Sbjct: 236 AETYKFHPETMLGQLVEIEMRDPTEVIPEEELKTWPKNIEVWNPAFDVTPPEYIDVIITE 295
Query: 338 KRVVWPPFKR--NLKKLFG 354
K ++ PP+ LK+ FG
Sbjct: 296 KGII-PPYAAIDILKEEFG 313
>gi|11499619 translation initiation factor eIF-2B, subunit delta (eif2BD)
[Archaeoglobus fulgidus]
>gi|3182986|sp|O28242|E2B2_ARCFU PUTATIVE TRANSLATION
INITIATION FACTOR EIF-2B SUBUNIT 2 (EIF-2B GDP-GTP
EXCHANGE FACTOR) >gi|7447866|pir||D69504 translation
initiation factor eIF-2B, subunit delta (eif2BD) homolog
- Archaeoglobus fulgidus >gi|2648498|gb|AAB89217.1|
(AE000962) translation initiation factor eIF-2B, subunit
delta (eif2BD) [Archaeoglobus fulgidus]
Length = 313
Score = 144 bits (360), Expect = 1e-33
Identities = 105/306 (34%), Positives = 158/306 (51%), Gaps = 31/306 (10%)
Query: 44 VESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRPTA 103
V AE I++M VRGA I AA L +AE + + DE M A ETL NTRPTA
Sbjct: 4 VLEAAEKIRSMEVRGAARIARFAAETLMKFAEKASDEKFDEEMRF---AAETLLNTRPTA 60
Query: 104 VNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEG-N 162
V+L+ A+N + + E +E E + ++ A++ + A ++G G + + +G
Sbjct: 61 VSLYNAINYVMRYSGESVE----EKRQSVIRRAREFINWVETAQRKIGEIGEKRIKDGYT 116
Query: 163 LLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNV 222
++THCN+ + +V++ H++G + E+RP QG L+ + G+ V
Sbjct: 117 VMTHCNSSAAL--------SVIKKAHENGKRVEVIATESRPRWQG-HLTVKQLREAGIEV 167
Query: 223 KLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLS 282
LI D+A + + + VD ++VGAD I ANG NKIGT +A+ A+E +PF A
Sbjct: 168 TLIVDSAVRYFINE--VDCVVVGADTITANGALINKIGTSQIALAAKEARVPFMVAAETY 225
Query: 283 SIDMSLKSGKEIPIEER-----SPEEVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGIITD 337
G+ + IEER +PEE+L G V V NPAFDVTP Y+ IIT+
Sbjct: 226 KFSPKTLFGELVVIEERDAREVAPEEILKLG-------VKVRNPAFDVTPRDYIDVIITE 278
Query: 338 KRVVWP 343
+ P
Sbjct: 279 IGAIPP 284
>gb|AAG20059.1| (AE005085) translation initiation factor eIF-2B subunit alpha;
Eif2ba [Halobacterium sp. NRC-1]
Length = 314
Score = 131 bits (326), Expect = 1e-29
Identities = 105/315 (33%), Positives = 153/315 (48%), Gaps = 18/315 (5%)
Query: 44 VESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRPTA 103
V + A+ I +M +RGA I AAA L A S A S F A L++TRPTA
Sbjct: 6 VRATADDIADMEIRGAATIARAAADALESQATDSDADSPAAFRAELRAAARHLRDTRPTA 65
Query: 104 VNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGN- 162
V+L N ++ ++ + + +++ + + A + A +G GA L +G+
Sbjct: 66 VSL---PNALRYVLGNTDGETVAALRASVTDAADAFRQQLDRAQDDLGAIGANRLRDGDT 122
Query: 163 LLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNV 222
++THC H A V+ DG + ETRP QG ++A + G+ V
Sbjct: 123 VMTHC--------HSTDALACVKAALDDGKHIEAVVKETRPRKQG-HITADQLREWGVPV 173
Query: 223 KLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLS 282
LI DNA + VD ++VGAD I A+G NKIGT MLAV+ARE G+P A
Sbjct: 174 TLIVDNAGRRYLDA--VDHVLVGADSIAADGSVINKIGTSMLAVVARERGVPVMVAAQTL 231
Query: 283 SIDMSLKSGKEIPIEERSPEEVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGIITDKRVVW 342
+ +G + IE R EVL V NPAFDVTP +YV I+T+ R +
Sbjct: 232 KLHPDTLTGNTVEIEMRDETEVLAAADRHQLGAPDVENPAFDVTPPRYVDAIVTE-RGQF 290
Query: 343 PP--FKRNLKKLFGE 355
PP +++L+G+
Sbjct: 291 PPESIVTLMRELYGD 305
>emb|CAA39062.1| (X55387) ORF3 [Rhodospirillum rubrum]
Length = 185
Score = 99.1 bits (243), Expect = 7e-20
Identities = 67/166 (40%), Positives = 90/166 (53%), Gaps = 23/166 (13%)
Query: 24 VYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKD 83
V++I+Q LP EF + + +VA AI+ M VRGAP IGA AA+G+AL ++D
Sbjct: 20 VWIIDQTRLPHEFVTQRLNDLGAVAHAIRAMLVRGAPLIGATAAYGVAL------GMAED 73
Query: 84 EFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEAQKIADED 143
+G +A +TL TRPTAVNL WA I+ + E P D+ +A I DED
Sbjct: 74 PSDEGLTRACQTLLATRPTAVNLRWA---IEAMAESLAAVPPDQRAQAAWAKAGAICDED 130
Query: 144 VEANLRMGHYGAEVLP------------EG--NLLTHCNAGSLATV 175
V N +G +G ++ EG N+LTHCNAG LATV
Sbjct: 131 VALNEAIGDHGLGIIKDLARTKGVEKGGEGPINILTHCNAGWLATV 176
>sp|P23140|YPE3_RHORU HYPOTHETICAL PROTEIN IN PETC 3'REGION >gi|79578|pir||S12260
hypothetical protein 3 (petC 3' region) - Rhodospirillum
rubrum (fragment)
Length = 184
Score = 99.1 bits (243), Expect = 7e-20
Identities = 67/166 (40%), Positives = 90/166 (53%), Gaps = 23/166 (13%)
Query: 24 VYMINQRLLPREFKVEAFRTVESVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKD 83
V++I+Q LP EF + + +VA AI+ M VRGAP IGA AA+G+AL ++D
Sbjct: 20 VWIIDQTRLPHEFVTQRLNDLGAVAHAIRAMLVRGAPLIGATAAYGVAL------GMAED 73
Query: 84 EFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLEDPLDEIKSLIVNEAQKIADED 143
+G +A +TL TRPTAVNL WA I+ + E P D+ +A I DED
Sbjct: 74 PSDEGLTRACQTLLATRPTAVNLRWA---IEAMAESLAAVPPDQRAQAAWAKAGAICDED 130
Query: 144 VEANLRMGHYGAEVLP------------EG--NLLTHCNAGSLATV 175
V N +G +G ++ EG N+LTHCNAG LATV
Sbjct: 131 VALNEAIGDHGLGIIKDLARTKGVEKGGEGPINILTHCNAGWLATV 176
>pir||T40858 translation initiation factor eif-2b alpha subunit - fission yeast
(Schizosaccharomyces pombe) >gi|6016995|emb|CAB57849.1|
(AL121783) translation initiation factor eif-2b alpha
subunit [Schizosaccharomyces pombe]
Length = 341
Score = 87.4 bits (213), Expect = 2e-16
Identities = 81/301 (26%), Positives = 132/301 (42%), Gaps = 36/301 (11%)
Query: 65 AAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRPTAVNLFWALNRIKKLVEEHLED- 123
AA L S+AK+ EFMD TLK ++L + ++ V L D
Sbjct: 38 AAIEALVQLLSRSQAKTISEFMDILQNGSNTLKEGVQNNISLSAGCDIFQRFVTRSLHDV 97
Query: 124 -PLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVG 181
++ K +V + R+ H G ++ +G+ +LTH + +A V L
Sbjct: 98 GDFEQCKRHLVENGKLFIQRARACRQRIAHLGYPLIRDGSVILTHGFSRGVAAVLLAAAK 157
Query: 182 AVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDA 241
VR +++ E+RP G ++ + ++ D+A +F M + VD
Sbjct: 158 RHVRFK--------VFVTESRPSGSGCLMTR-TLKNACIPTCMVLDSAVSFTMNR--VDL 206
Query: 242 IIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVA---------PLSSIDMSLKSGK 292
++VGA+ +V NG N+IGT+ LAV A+ PF+AVA PLS D+ S
Sbjct: 207 VLVGAEGVVENGGLINQIGTFQLAVFAKHAHKPFYAVAESHKFVRMFPLSQYDIPF-SRP 265
Query: 293 EIPIEERSPEEVLTCGGCRIAPDVPVY------------NPAFDVTPHKYVTGIITDKRV 340
+ ++ SPE V P ++ NP DVTP ++V+G+ITD +
Sbjct: 266 ILEFDDPSPETVHPEPEPIPTPSDAIHNELIMNEEQIRNNPTLDVTPPEFVSGLITDLGI 325
Query: 341 V 341
+
Sbjct: 326 I 326
>sp|O58185|E2BL_PYRHO PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2-LIKE
(EIF-2B GDP-GTP EXCHANGE FACTOR) >gi|7521497|pir||A71155
probable translation initiation factor eIF-2B -
Pyrococcus horikoshii >gi|3256843|dbj|BAA29526.1|
(AP000002) 276aa long hypothetical translation
initiation factor eIF-2B [Pyrococcus horikoshii]
Length = 276
Score = 86.6 bits (211), Expect = 4e-16
Identities = 65/222 (29%), Positives = 106/222 (47%), Gaps = 23/222 (10%)
Query: 124 PLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVGA 182
P+ + ++ + A + EA + GA+++ +G+ ++TH + TV
Sbjct: 73 PVTNRRDILKSRALEFLRRMEEAKRELASIGAQLIDDGDVIITHSFSS--------TVLE 124
Query: 183 VVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAI 242
++R + + L E+ P +G L A E + G+ ++I D ++ +
Sbjct: 125 IIRTAKERKKRFKVILTESSPDYEGLHL-ARELEFSGIEFEVITDAQMGLFCREASI--A 181
Query: 243 IVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPE 302
IVGAD I +G NK GTY+LA+ E+ IPF+ A +LKSG ++ + ER
Sbjct: 182 IVGADMITKDGYVVNKAGTYLLALACHENAIPFYVAAETYKFHPTLKSG-DVMLMERD-- 238
Query: 303 EVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGIITDKRVVWPP 344
I +V + N FDVTP KYV GIIT+ +V PP
Sbjct: 239 --------LIRGNVRIRNVLFDVTPWKYVRGIITELGIVIPP 272
>gb|AAF27156.1|AC016529_19 (AC016529) putative translation initiation factor EIF-2B alpha
subunit; 76271-74747 [Arabidopsis thaliana]
Length = 362
Score = 76.5 bits (185), Expect = 4e-13
Identities = 82/304 (26%), Positives = 136/304 (43%), Gaps = 23/304 (7%)
Query: 46 SVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRPTAVN 105
+V E N + A AA LA S+A + E KA +TLK+ T+++
Sbjct: 64 NVIEEFNNWRKQPDLAEAVAAIRALAAVIRASEASTMMELEIELKKASDTLKSWDKTSIS 123
Query: 106 LFWALNRIKKLVEEH--LE-DPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEG- 161
L + + V LE + + KS ++ A+K + +A + + + +G
Sbjct: 124 LTAGCDLFIRYVTRTSALEYEDFNSAKSRLLERAEKFGEISCKARRIIAMLSQDFIFDGC 183
Query: 162 NLLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLN 221
+L H L+ V L +++ ++ L + E RP G LS+ E S +
Sbjct: 184 TILVH----GLSRVVL----EILKTAAQNNKLFRVLCTEGRPDGTGVLLSS-ELSKLDIP 234
Query: 222 VKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPL 281
VKL+ D+A A+ M + VD + VGAD +V +G N +GTY +A++A P + A
Sbjct: 235 VKLLLDSAVAYSMDE--VDMVFVGADGVVESGGIINMMGTYQIALVAHSMNKPVYVAAE- 291
Query: 282 SSIDMSLKSGKEIPIEERSPEEVLTC--GGCRIAPDVPVYNPAFDVTPHKYVTGIITDKR 339
S K + P++++ L G +I V V A D TP +Y+T + TD
Sbjct: 292 -----SYKFARLYPLDQKDMAPALRPIEFGVKIPTKVEVERSARDYTPPQYLTLLFTDLG 346
Query: 340 VVWP 343
V+ P
Sbjct: 347 VLSP 350
>gb|AAF02860.1|AC009324_9 (AC009324) Similar to initiation factor 2 subunit [Arabidopsis
thaliana] >gi|6056397|gb|AAF02861.1|AC009324_10
(AC009324) Similar to initiation factor 2 subunit
[Arabidopsis thaliana]
Length = 351
Score = 73.7 bits (178), Expect = 3e-12
Identities = 79/305 (25%), Positives = 133/305 (42%), Gaps = 25/305 (8%)
Query: 46 SVAEAIKNMTVRGAPAIGAAAAFGLALYAETSKAKSKDEFMDGFYKAYETLKNTRPTAVN 105
SV E + + A AA LA S+A + E KA +TLK+ T+++
Sbjct: 53 SVIEDFNSWRQQPDLAEAVAAIRALAAVIRASEATTMMELEIELKKASDTLKSWDTTSIS 112
Query: 106 LFWALNRIKKLVEE----HLEDPLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEG 161
L + + V ED + KS ++ A+K + +A + + + +G
Sbjct: 113 LTAGCDLFMRYVTRTSALEFED-FNSAKSRVLERAEKFGEISCKARTIIAMLSQDFIFDG 171
Query: 162 -NLLTHCNAGSLATVHLGTVGAVVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGL 220
+L H + V +++ ++ L + E RP G L A E + +
Sbjct: 172 CTILVHGFSR--------VVFEILKTSAQNKKLFRVLCTEGRPDKTGVLL-ANELAKLDI 222
Query: 221 NVKLIADNAAAFVMQQGLVDAIIVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAP 280
VKL+ D+A A+ M + VD + VGAD +V +G N +GTY +A++A+ P + A
Sbjct: 223 PVKLLIDSAVAYSMDE--VDMVFVGADGVVESGGIINMMGTYQIALVAQSMNKPVYVAAE 280
Query: 281 LSSIDMSLKSGKEIPIEERSPEEVL--TCGGCRIAPDVPVYNPAFDVTPHKYVTGIITDK 338
S K + P++++ E L + P V V A D TP +Y+T + TD
Sbjct: 281 ------SYKFARLYPLDQKDLEPALRPIDFSVPVPPKVEVERSARDYTPPQYLTLLFTDL 334
Query: 339 RVVWP 343
V+ P
Sbjct: 335 GVLTP 339
>sp|Q9UYB6|E2BL_PYRAB PUTATIVE TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT 2-LIKE
(EIF-2B GDP-GTP EXCHANGE FACTOR) >gi|7521496|pir||B75007
probable translation initiation factor aif-2bII
translation initiation factor PAB1306 - Pyrococcus
abyssi (strain Orsay) >gi|5459010|emb|CAB50496.1|
(AJ248288) putative, translation initiation factor
aIF-2BII translation initiation factor [Pyrococcus
abyssi]
Length = 276
Score = 73.7 bits (178), Expect = 3e-12
Identities = 61/222 (27%), Positives = 106/222 (47%), Gaps = 23/222 (10%)
Query: 124 PLDEIKSLIVNEAQKIADEDVEANLRMGHYGAEVLPEGN-LLTHCNAGSLATVHLGTVGA 182
P+ + L+ A + EA + GA+++ +G+ ++TH + S+ +
Sbjct: 73 PITNNRELVKMRALEFLRRMDEAKRELASIGAQLIDDGDVIITHSFSSSVLEIF-----K 127
Query: 183 VVRVMHKDGSLKLLWLDETRPVLQGARLSAWEYSYDGLNVKLIADNAAAFVMQQGLVDAI 242
V + K S K++ + E+ P +G L A E G+ ++I D+ ++ +
Sbjct: 128 VAKDRRK--SFKVI-ITESSPDYEGLHL-ANELENLGIEFEVITDSQMGLFCRKATIS-- 181
Query: 243 IVGADRIVANGDFANKIGTYMLAVLAREHGIPFFAVAPLSSIDMSLKSGKEIPIEERSPE 302
+VGAD + +G NK GTY+LA+ E +PF+ A ++KS E+ + ER
Sbjct: 182 MVGADMVTRDGFVVNKAGTYLLALACHESDVPFYVAAETYKFHPTVKS-NEVVLHERD-- 238
Query: 303 EVLTCGGCRIAPDVPVYNPAFDVTPHKYVTGIITDKRVVWPP 344
+ G R V N FD+TP K++ GIIT+ +V PP
Sbjct: 239 --FSRSGFR------VRNVLFDLTPWKFIRGIITELGIVIPP 272
Database: ./suso.pep
Posted date: Jul 6, 2001 5:57 PM
Number of letters in database: 840,471
Number of sequences in database: 2977
Database: /banques/blast2/nr.pep
Posted date: Dec 14, 2000 12:46 PM
Number of letters in database: 188,266,275
Number of sequences in database: 595,510
Lambda K H
0.319 0.136 0.394
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 128096610
Number of Sequences: 2977
Number of extensions: 5180695
Number of successful extensions: 13981
Number of sequences better than 1.0e-10: 32
Number of HSP's better than 0.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 13871
Number of HSP's gapped (non-prelim): 33
length of query: 356
length of database: 189,106,746
effective HSP length: 57
effective length of query: 299
effective length of database: 154,992,987
effective search space: 46342903113
effective search space used: 46342903113
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 165 (68.7 bits)