19

Mining Archaeal Proteomes for Eukaryotic Proteins with Novel Functions

Orthologs in Archaea	Gene	Blast Expect	Paralogs
Pyrococcus abyssi	PAB1852	1e-132
Pyrococcus furiosus	Pf_1195239	1e-114
Pyrococcus horikoshii	PH1379	1e-122
Aeropyrum pernix	APE0502	2e-19
Archaeoglobus fulgidus	AF0734	9e-36
Methanobacterium thermoautotrophicum
Methanococcus jannaschii	MJ0557	1e-39
Homologs in Eucarya
Human	AAC51641.1 ( C2f )
Yeast	Q10107 S. pompe, Ylr186wp S. cerevisiae
Other	CAB72170.1 A.thaliana, AAF35325.1 C. albicans, Y39A1A.14 C. elegans, NP_038564.1 M. musculus, AAF45956.1 D. melanogaster

Size of P. abyssi protein	232 aa.
Best Hits outside Archaea	Gene	Blast Expect
Best Eucaryotic Hit	Q10107Mra1 Suppressor Protein	3e-16	S. pompe
Best Bacterial Hit	AAA96399.1	3.0	Synechocystis sp.

Module (Pfam, Prosite, Prints)
PRODOM module	Multicopy suppressor of RAS1/MRA1/pheromone reponse XII
PDB hit
COG group	COG1756: Uncharacterized conserved protein
KOG group	KOG3073 (Hs15625590) = Protein required for 18S rRNA maturation and 40S ribosome biogenesis. Human homologue is C2f protein, members of this family are essential for 40S ribosomal biogenesis as they interfere with a methylation reaction during the early stages of pre-rRNA processing necessary for the generation of ribosomal subunits.
Gene Environment in Archaea	Not conserved	See Environment
Phylogeny	See Unrooted Distance Tree

Function Prediction	uncharacterized

Red= new annotations