5

Mining Archaeal Proteomes for Eukaryotic Proteins with Novel Functions

PACE 5

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Orthologs in Archaea	Gene	Blast Expect	Paralogs
Pyrococcus abyssi	PAB0406	1e-123
Pyrococcus furiosus	Pf_1476752	1e-115
Pyrococcus horikoshii	PH1566	1e-114
Aeropyrum pernix	APE0754	8e-27
Archaeoglobus fulgidus	AF1805	4e-33
Methanobacterium thermoautotrophicum	MTH1006	3e-47
Methanococcus jannaschii	MJ0443	7e-43
Homologs in Eucarya
Human	NP_008974.1
Yeast	T38522 S. pombe
Other	PFB0370c  P. falciparum, AAC47659.1 N. locustae, T31743 C. elegans
Size of P. abyssi protein	221 aa.
Best Hits outside Archaea	Gene	Blast Expect
Best Eucaryotic Hit	PFB0370c	4e-11	P. falciparum
Best Bacterial Hit	TM1857	0.002	T. maritima
Module (Pfam, Prosite, Prints)	PS50084 : KH : RNA metabolism
PRODOM domain	polyribonucleotide/REV interacting RIP-1
PDB hit
COG group	COG1094 : Pred. RNA-binding proteins (S1 and KH domain)
KOG group	KOG3273 (Hs10047140) = predicted RNA-binding protein. Yeast homologue is Pno1 interacting with Nob1 and involved in 26S proteasome assembly KOG2874 (Hs21359980, HsM20127513) = rRNA processing protein.Yeast homologue is KRR1, involved in 40S ribosome assembly in the nucleolus. Human homologue is HRB2/RIP1 (human immunodeficiency virus type 1 Rev-binding protein 2), belonging to a family of serine/threonine kinases involved in signal transdution
Gene Environment in Archaea	CONSERVED : informational aIF-1A//PACE 4-PACE 5//DNA topo		See Environment
Phylogeny	See Unrooted Distance Tree
Function Prediction	Informational

Red= new annotations