9

Mining Archaeal Proteomes for Eukaryotic Proteins with Novel Functions

PACE 9

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Orthologs in Archaea	Gene	Blast Expect	Paralogs
Pyrococcus abyssi	PAB0746	0.0
Pyrococcus furiosus	Pf_1017484	1e-153
Pyrococcus horikoshii	PH1105	1e-162
Aeropyrum pernix	APE0447	1e-35
Archaeoglobus fulgidus	AF1803	2e-63
Methanobacterium thermoautotrophicum	MTH1319	2e-63
Methanococcus jannaschii	MJ0483	2e-42
Homologs in Eucarya
Human	DPH2L, OVCA1 candidate tumor suppressor genes
Yeast	YIL103w S. cerevisiae, SPBC3B8.05 S. pombe
Other	C14B1.5 C. elegans, AAF50025.1 AAF55111.1 D. melanogaster, CAB75460.1 A. thaliana
Size of P. abyssi protein	339 aa.
Best Hits outside Archaea	Gene	Blast Expect
Best Eucaryotic Hit	C14B1.5	2e-26	C. elegans
Best Bacterial Hit	AAD44403.1	0.55	T. aquaticus
Module (Pfam, Prosite, Prints)	PF01866 : Putative diphtamide synthesis protein
PRODOM domain	PD004399 : Diptheria toxin resistance diphtamide biosynthesis
PDB hit
COG group	COG1736 : Diphthamide synthase subunit DPH2
KOG group	KOG2648 (Hs21536476, DPH2; Hs4503361, DPH-2like or OVCA1) = Diphtamide biosynthesis protein (diphtamide is a post-translationally modified histidine residue present in elongation factor EF2 that is the target of diphteria toxin ADP-ribosylation). OVCA1 is a candidate tumor suppressor, since it is reduced in tumors and inhibits growth of ovarian cancer cells, it maps to a highly conserved region on chromosome 17p13.3 that shows frequent loss of heterozygosity in breast and ovarian carcinomas.
Gene Environment in Archaea	CONSERVED: informational helicase/ ribosomal proteins		See Environment
Phylogeny	See Unrooted Distance Tree
Function Prediction	Uncharacterized