11

Mining Archaeal Proteomes for Eukaryotic Proteins with Novel Functions

Orthologs in Archaea	Gene	Blast Expect	Paralogs
Pyrococcus abyssi	PAB0944	5e-85
Pyrococcus furiosus	Pf_1033428	1e-71
Pyrococcus horikoshii	PH0624	7e-78
Aeropyrum pernix
Archaeoglobus fulgidus	AF2206	1e-18
Methanobacterium thermoautotrophicum	MTH1896	1e-25
Methanococcus jannaschii	MJ1030	1e-25
Homologs in Eucarya
Human
Yeast	YGR277c S. cerevisiae, C1F12.08 S. pombe
Other	AAD15511.1 A. thaliana

Size of P. abyssi protein	157 aa.
Best Hits outside Archaea	Gene	Blast Expect
Best Eucaryotic Hit	YGR277c	7e-17	S. cerevisiae
Best Bacterial Hit	aq_1368	0.001	A. aeolicus

Module (Pfam, Prosite, Prints)	PF01467 : Cytidylyltransferase
PRODOM domain	Transferase cytidyltransferase nucleotidyltransderase
PDB hit	CTP : glycerol-3-phosphate cytidylyltranferase
COG group	COG1019 : Predicted nucleotidyltransferase
KOG group	KOG3351 (Hs21489977) = Predicted nucleotidyltransferase PPAT (Armangaud J et al. 2003)
Gene Environment in Archaea	Not conserved: NIFS-like/transcription regulation/(DNA topo)	See Environment
Phylogeny	See Unrooted Distance Tree

Function Prediction	Uncharacterized

Red= new annotations