13

Mining Archaeal Proteomes for Eukaryotic Proteins with Novel Functions

PACE 13

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Orthologs in Archaea	Gene	Blast Expect	Paralogs
Pyrococcus abyssi	PAB1006	4e-41
Pyrococcus furiosus	Pf_389836	2e-27
Pyrococcus horikoshii	PH0527	2e-35
Aeropyrum pernix
Archaeoglobus fulgidus	AF2063	2e-03
Methanobacterium thermoautotrophicum	MTH1609	1e-07
Methanococcus jannaschii	MJ0952	4e-05
Homologs in Eucarya
Human	NP_002615.1 C-Myc Binding Protein
Yeast	YML094w S. cerevisiae
Other	AAD28373.1 BAA92269.1 M. musculus, AAF56007.1 D. melanogaster
Size of P. abyssi protein	148 aa.
Best Hits outside Archaea	Gene	Blast Expect
Best Eucaryotic Hit	YML094w	7e-10	S. cerevisiae
Best Bacterial Hit	aq_759	8e-09	A. aeolicus
Module (Pfam, Prosite, Prints)
PRODOM domain	c-myc binding/repressor of its transcriptional activity
PDB hit
COG group	COG1730 : GIM5 Predicted prefoldin, molecular chaperone implicated in de novo protein folding
KOG group	KOG3048 (Hs4505743) = Molecular chaperone Prefoldin, subunit 5. Human homologue is MM-1, a nuclear c-Myc –binding protein. MM-1 is a possible tumor suppressor controlling the transcriptional activity of c-Myc (one frequent A157R mutation in leukemia/linphoma cells and squamous carcinoma of tongue cancer. It maps to chromosome 12Q12-12q13, where many abnormalities have been reported in cancer cells. It is associated also to p73 alpha, it may act as a molecular partner for p73 to prevent the c-Myc-mediated inhibitory effect on its activity.
Gene Environment in Archaea	HIGHLY CONSERVED: ribosomal proteins/EIF-6/rplXA		See Environment
Phylogeny	See Unrooted Distance Tree
Function Prediction	Informational : translation