32

Mining Archaeal Proteomes for Eukaryotic Proteins with Novel Functions

PACE 32

Orthologs in Archaea

Gene

Blast Expect

Paralogs

Pyrococcus abyssi

PAB1506

5e-94

Pyrococcus furiosus

Pf_1063642

1e-88

Pyrococcus horikoshii

PH0734

1e-87

Aeropyrum pernix

APE0525

3e-09

Archaeoglobus fulgidus

AF1405

2e-25

Methanobacterium thermoautotrophicum

MTH650

1e-24

Methanococcus jannaschii

MJ1432

2e-34

Homologs in Eucarya

Human

MCT-1 protein

Yeast

Yer007c-ap S. cerevisiae, SPBC31F10.12 S. pombe

Other

Size of P. abyssi protein

172 aa.

Best Hits outside Archaea

Gene

Blast Expect

Best Eucaryotic Hit

BAA86055.1

1e-11

H. sapiens

Best Bacterial Hit

Module (Pfam, Prosite, Prints)

PF01472 : PUA domain (PseudoUridine synthase and Archaeosine transglycosylase)

PRODOM module

PDB hit

COG group

COG2016 : Predicted RNA-binding protein

KOG group KOG2523 (Hs7662502) = Predicted RNA-binding protein. Human homologue is candidate oncogene MCT-1, with unknown function, possibly a DNA damage-response gene, since it is over-expressed after irradiation. Its expression is elevated in some samples of aggressive lymphoma, including large B-cell lymphoma (DLBCL) and interleukin-2 (IL-2)-independent T-cell lymphoid tumors. Expression levels are stongly associated with cell proliferation marker PCNA. High levels of MCT-1 protein may be associated with a high-risk subset of lymphoid neoplasms

Gene Environment in Archaea

Not conserved

See Environment

Phylogeny

See Unrooted Distance Tree

Function Prediction

uncharacterized

Red= new annotations

Homologs in Eucarya
Orthologs in Archaea	Gene	Blast Expect	Paralogs
Pyrococcus abyssi	PAB1506	5e-94
Pyrococcus furiosus	Pf_1063642	1e-88
Pyrococcus horikoshii	PH0734	1e-87
Aeropyrum pernix	APE0525	3e-09
Archaeoglobus fulgidus	AF1405	2e-25
Methanobacterium thermoautotrophicum	MTH650	1e-24
Methanococcus jannaschii	MJ1432	2e-34
Human	MCT-1 protein
Yeast	Yer007c-ap S. cerevisiae, SPBC31F10.12 S. pombe
Other

Size of P. abyssi protein	172 aa.
Best Hits outside Archaea	Gene	Blast Expect
Best Eucaryotic Hit	BAA86055.1	1e-11	H. sapiens
Best Bacterial Hit

Module (Pfam, Prosite, Prints)	PF01472 : PUA domain (PseudoUridine synthase and Archaeosine transglycosylase)
PRODOM module
PDB hit
COG group	COG2016 : Predicted RNA-binding protein
KOG group	KOG2523 (Hs7662502) = Predicted RNA-binding protein. Human homologue is candidate oncogene MCT-1, with unknown function, possibly a DNA damage-response gene, since it is over-expressed after irradiation. Its expression is elevated in some samples of aggressive lymphoma, including large B-cell lymphoma (DLBCL) and interleukin-2 (IL-2)-independent T-cell lymphoid tumors. Expression levels are stongly associated with cell proliferation marker PCNA. High levels of MCT-1 protein may be associated with a high-risk subset of lymphoid neoplasms
Gene Environment in Archaea	Not conserved		See Environment
Phylogeny	See Unrooted Distance Tree

Function Prediction	uncharacterized