Biology of Extremophiles
	Jonathan Filée   Curiculum vitae   Born in Charleroi (Belgium) 20/12/1976 33 bis Villa de Chennevières 78700, Conflans-Sainte-Honorine 06 67 68 16 53 jonathan.filee@igmors.u-psud.fr     Education : 1998 : Masters degree in Biochemistery, Université de Cergy-Pontoise (France). Present : PhD degree, Université Paris-Sud Orsay (France), Institut de Génétique et Microbiologie, Laboratoire de Biologie moléculaire des Extrémophiles. PhD supervisor : Patrick Forterre. Subject : Phylogeny of viral protein involved in the replication and the metabolism of DNA.   Work experience : 1999 : 7-months internship in the "Unité de Physiologie Microbienne" (Institut Pasteur, France) : Study of the 16S-23S ribosomal intergenic region of toxic Cyanobacteria by PCR-RFLP. DNA sequencing of this region and phylogeny. Primers designing to identify specifically toxic Cyanobacteria. 2000 : 6-months internship in the "Laboratoire de Biologie Moléculaire du gène chez les Extrêmophiles" (IGM Orsay, France): Bioinformatic, sequence analysis of virus, molecular phylogeny and evolutionary consequences on the mecanisms of the DNA replication.   Skills : Molecular Biology : PCR, Cloning, Sequencing, RFLP. Computing : Phylogeny (Molphy, PAUP, Puzzle, Phylip...), Sequence analysis (BLAST, Clustal, COG, MUST), UNIX (Linux, C-programming language), DOS (Windows).   Presentations and abstracts : 2000 : Origins, Diversity, and Evolution of Viruses. Fondation Des Treilles (France). Lecture : "Early or late virus ? A view on viral origin from DNA polymerase phylogenies." Jonathan Filée, Jacqueline Laurent & Patrick Forterre. 2001 : School "Exobiology". CNRS. La Colle sur Loup (France). 2002 : The Molecular Evolution Meeting. Sorrento (Italy). Poster : "What viral gene tel us about the evolution of the DNA world". Jonathan Filée, Patrick Forterre & Jacqueline Laurent 2002: The Thirteen International Conference on Origin of Life. Oaxaca (Mexico). Poster : "Origin of DNA and DNA genome." Patrick Forterre, Hannu Myllykallio & Jonathan Filée.   Publications : Filee J, Forterre P, Sen-Lin T, Laurent J (2002) Evolution of DNA polymerase families: evidences for multiple gene exchange between cellular and viral proteins. J Mol Evol 54:763-73. Myllykallio H, Lipowsky G, Leduc D, Filee J, Forterre P, Liebl U (2002) An alternative flavin-dependent mechanism for thymidylate synthesis. Science 5578:105-7. Gadelle D, Filee J, Buhler C, Forterre P (2003) Phylogenomics of type II DNA topoisomerases. BioEssay 25 : 232-242. Forterre P, Filee J, Myllykalio H (2003) Origin and evolution of DNA and DNA replication machineries. In "The Genetic Code and the Origin of Life" L. Ribas, ed. Landes Bioscience. Myllykallio H, Leduc D, Filee J, Liebl U (2003) Life without dihydrofolate reductase FolA. Trends Microbiol. In press. Filee J, Forterre P, Laurent J (2003) The role played by viruses on the Evolution of their cellular host : a view on informational proteins phylogenies. Res. Microbiol. In press.   Thesis : Filee J (2002) Phylog�nie mol�culaire des g�nes viraux impliqu�s dans le m�tabolisme et la r�plication de l'ADN. Th�se de l'Universit� Paris-Sud.