From Genome to Life:

BELLI Gemma

University of Lleida, Basic Medical Sciences, Avgda. Alcalde Rovira Roure, 44, Lleida 25198, Spain

title: Genomic analysis of a family of Zn(2)-Cys(6)-cluster domain transactivators in the yeast Saccharomyces cerevisiae

The yeast Saccharomyces cerevisiae contains a family of three ORFs (YJL103c,YPL133c, YBR239c) coding for proteins that have a Zn(2)-Cys(6) binuclear cluster domain at the N-terminal region. This domain is characteristic of fungal species and the closest described homologues to the above three ORFs are in Candida albicans. We are carrying out a genomic and functional analysis of the three transactivators, using the tetracycline-regulatable tet promoter to study the effect of overexpression of each of the three ORFs on the yeast transcriptome. Microscopy analysis with HA-tagged proteins has confirmed that they are located at the nucleus, although the YPL133c product seems to be located at the cytoplasm during exponential phase, nuclear location being exclussive of post-diauxic phase. This poits to the existence of a post-translational regulation on this protein. Phenotypic studies of individual and mutants are also being carried out.