BLAST
K. lactis Query Page

GAPPED-BLAST search in LBMGE Genomes DataBase.

Similarity searches are performed with GAPPED-BLAST (BLAST 2.2.3 [May-13-2002] NCBI, Altschul et al., 1997, Nucleic Acids Res. 25:3389-3402)

Kluyveromyces lactis Homepage

Select at least one Genome DataBase and the appropriate tool and type your query sequence below

   Display full alignements in graphics

Pairwise Master-slave with identities Master-slave no identities Flat master-slave Flat master-slave, no identities Master-slave no identities & blunt ends Flat master-slave, no identities & blunt ends
Output Alignment: (options not available with blastx and tblastx)

Genome (n) orfs (a) orfs (n) orfs (a) -full
(n= Nucleic ) (a= Amino )

blastn: n<->n 
blastp: a<->a 
blastx: n<->a  

tblastn: a<->n 
tblastx: n<->n

Select Genomes within groups:

Bacteriophages complete genomes

Genome (n) orfs (a) orfs (n) --

You can select genomes by groups or individually .
Check one or several checkboxes to toggle selected groups. (Javascript must be activated-)

Blast Advanced Options:

Expect value (E)

  [Real] 

Matrix

  BLOSUM30 BLOSUM35 BLOSUM40 BLOSUM45 BLOSUM50 BLOSUM55 BLOSUM60 BLOSUM62 BLOSUM65 BLOSUM70 BLOSUM75 BLOSUM80 BLOSUM85 BLOSUM90 PAM40 PAM120 PAM250

Filter query sequence (DUST with blastn, SEG with others)

  True False

Cost to open a gap

  [Integer]

Cost to extend a gap

  [Integer]

X dropoff value for gapped alignment (in bits)

  [Integer]

Reward for a nucleotide match

  [Integer] (blastn only)

Number of one-line descriptions (V)

  [Integer]

Number of alignments to show (B)

  [Integer]

Threshold for extending hits

  [Integer]

Penalty for a nucleotide mismatch (blastn only)

  [Integer]

Perform gapped alignment

  True False (not available with tblastx)